FastQCFastQC Report
Wed 3 Jan 2024
SRR9595476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9595476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26189965
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC16000596.10943542688965TruSeq Adapter, Index 9 (100% over 50bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC860170.3284349559077303TruSeq Adapter, Index 9 (98% over 50bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC650010.2481904805905621TruSeq Adapter, Index 9 (98% over 50bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC599600.22894265036245753TruSeq Adapter, Index 9 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATATCGTATGC270970.10346329214262026TruSeq Adapter, Index 9 (98% over 50bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)