FastQCFastQC Report
Wed 3 Jan 2024
SRR9595489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9595489_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24061770
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT1777810.7388525449291553TruSeq Adapter, Index 22 (97% over 40bp)
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA458400.19050967572211022No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAG415760.1727886186261443No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGGTCCCGAAGGCCAACACAATA313150.13014420801129759No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC266830.11089375386764981No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCGGCGGTGTCCTAAG265910.11051140460572934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)