FastQCFastQC Report
Wed 3 Jan 2024
SRR9595494_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9595494_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25509737
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT694130.2721039421143385TruSeq Adapter, Index 14 (97% over 44bp)
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA657680.25781528049465974No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAG647460.25380896714066475No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA481900.18890825883465598No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGGTCCCGAAGGCCAACACAATA433460.1699194311568167No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCGGCGGTGTCCTAAG426730.16728122285227792No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC392660.15392553831503633No Hit
CTGACATGTGTGCGAGTCGACGGGCGAGTAAACCCGTAAGGCGCAAGGAA390250.1529808010172743No Hit
CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTT369560.14487017251491066No Hit
GTCTGACATGTGTGCGAGTCGACGGGCGAGTAAACCCGTAAGGCGCAAGG344800.13516407480014395No Hit
CTCGATTCCGTGGCGCGGCTCAACGGAGCAGCCGCGCCGTCCTACCTATT332380.1302953456556608No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA331700.13002878077496446No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGT324330.12713968787682917No Hit
CGACGGGCGAGTAAACCCGTAAGGCGCAAGGAAGCTGACTGGCGGGATCC297780.11673189731434706No Hit
CCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAAT296440.11620660769650429No Hit
ACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGAT291840.11440337468002905No Hit
CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC279050.10938960287987289No Hit
CTCCTACTCATCGGGGCATGGCAATCGCCCCGACGGCCGGGTATGGGTCG261320.10243931562289334No Hit
CTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAATT256770.10065568296529281No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)