FastQCFastQC Report
Wed 3 Jan 2024
SRR9595495_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR9595495_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23226317
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT777800.33487874982503685TruSeq Adapter, Index 25 (97% over 44bp)
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA582130.25063379613737297No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAG538590.23188781932150498No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC436970.18813572552204466No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCGGCGGTGTCCTAAG378790.16308655392932078No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGGTCCCGAAGGCCAACACAATA306560.13198820975361697No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGT304980.1313079469293388No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA265640.11437026369699509No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA247570.1065902958269277No Hit
CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTT237380.10220303115642485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)