##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR9595473_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24689555
Sequences flagged as poor quality	0
Sequence length	35-151
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.900903033691776	32.0	32.0	32.0	32.0	32.0
2	30.634542299365055	32.0	32.0	32.0	32.0	32.0
3	30.72145577350422	32.0	32.0	32.0	32.0	32.0
4	30.7224407244278	32.0	32.0	32.0	32.0	32.0
5	30.727737093681924	32.0	32.0	32.0	32.0	32.0
6	34.03461868794314	36.0	36.0	36.0	32.0	36.0
7	34.23990189373603	36.0	36.0	36.0	32.0	36.0
8	34.08291664228051	36.0	36.0	36.0	32.0	36.0
9	34.08550141952741	36.0	36.0	36.0	32.0	36.0
10-14	33.964423149789454	36.0	36.0	36.0	32.0	36.0
15-19	33.90713656037948	36.0	36.0	36.0	32.0	36.0
20-24	33.80049873721904	36.0	36.0	36.0	32.0	36.0
25-29	33.656317134917984	36.0	36.0	36.0	29.0	36.0
30-34	33.531578758709905	36.0	36.0	36.0	25.8	36.0
35-39	33.40187768055736	36.0	36.0	36.0	21.0	36.0
40-44	33.274832415191916	36.0	36.0	36.0	19.6	36.0
45-49	33.101900170558395	36.0	36.0	36.0	14.0	36.0
50-54	32.88486543023006	36.0	36.0	36.0	14.0	36.0
55-59	32.66501185595819	36.0	34.4	36.0	14.0	36.0
60-64	32.44386133024587	36.0	32.0	36.0	14.0	36.0
65-69	32.247684908384635	36.0	32.0	36.0	14.0	36.0
70-74	32.04551651262494	36.0	32.0	36.0	14.0	36.0
75-79	31.83300819833745	36.0	32.0	36.0	14.0	36.0
80-84	31.62449343352595	36.0	32.0	36.0	14.0	36.0
85-89	31.44418157091842	36.0	32.0	36.0	14.0	36.0
90-94	31.25044905451591	36.0	32.0	36.0	14.0	36.0
95-99	31.068780575120012	36.0	32.0	36.0	14.0	36.0
100-104	30.805711163530567	36.0	27.0	36.0	14.0	36.0
105-109	30.544227992371145	36.0	27.0	36.0	14.0	36.0
110-114	30.187466273110296	36.0	27.0	36.0	14.0	36.0
115-119	29.758485870321795	32.0	27.0	36.0	14.0	36.0
120-124	29.259605880567584	32.0	27.0	36.0	14.0	36.0
125-129	28.724936554416047	32.0	27.0	36.0	14.0	36.0
130-134	28.19336885144441	32.0	24.6	36.0	14.0	36.0
135-139	27.67635317141575	32.0	21.0	36.0	14.0	36.0
140-144	27.332360564123576	32.0	21.0	32.8	14.0	36.0
145-149	27.024131250491696	32.0	21.0	32.0	14.0	36.0
150-151	24.593504306994024	26.5	17.5	29.5	14.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3911.0
3	101.0
4	17.0
5	16.0
6	30.0
7	112.0
8	235.0
9	371.0
10	401.0
11	409.0
12	463.0
13	402.0
14	3736.0
15	17792.0
16	32685.0
17	43816.0
18	51362.0
19	65454.0
20	94505.0
21	142990.0
22	215431.0
23	314413.0
24	440729.0
25	597077.0
26	784595.0
27	1006082.0
28	1263019.0
29	1577260.0
30	1995587.0
31	2595952.0
32	3480573.0
33	4653786.0
34	4531926.0
35	774317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.938536116853147	13.195346966384335	16.912370308821234	46.953746607941284
2	21.8984416948336	19.480127150843803	31.907308840059272	26.714122314263328
3	26.047757010280925	21.593861284046053	22.531438898170794	29.826942807502228
4	27.476011276343787	26.62919579103027	18.469317066292046	27.425475866333905
5	27.078230593248918	30.045493996011857	21.93194231067208	20.944333100067144
6	22.48639915361258	34.26423606839174	21.81141361501307	21.437951162982607
7	19.516681827453034	19.7316834508826	39.46271874523377	21.288915976430598
8	21.32496608507348	22.579777856421085	28.36563110282332	27.72962495568212
9	22.169502890209937	22.14428538262413	30.434951165739548	25.251260561426385
10-14	22.735405574582863	27.808699732685188	25.304477971385005	24.151416721346948
15-19	23.252207261378537	26.353434971959445	26.63099600632567	23.763361760336352
20-24	23.4302429903731	26.57896939171668	26.559549570345975	23.43123804756425
25-29	23.02243844681166	27.145141039277487	25.76094047259228	24.071480041318576
30-34	23.028575536070903	26.545607723560323	26.284890549405965	24.140926190962812
35-39	22.78485989619078	26.832651902528852	25.974284737517728	24.408203463762636
40-44	23.39625563248419	26.47936388253519	26.294080956242517	23.830299528738102
45-49	23.24514709841723	26.549786649839223	26.582004192567148	23.623062059176394
50-54	23.596000302010662	26.771809996784672	25.849697734475143	23.782491966729523
55-59	23.068635643125184	26.88735905090489	26.106648899816292	23.93735640615363
60-64	23.27823845740939	26.496170941919356	26.15275724681934	24.072833353851912
65-69	23.32108276095569	26.693464638391106	26.115947149658048	23.869505450995156
70-74	23.161810177605442	26.938238723337633	25.92677970988445	23.973171389172474
75-79	23.600286854789218	26.56181538227394	25.527218652795657	24.310679110141184
80-84	23.528049150639667	26.775934252427234	25.665623737223097	24.030392859710005
85-89	23.364752277858543	26.465127394367137	26.028009999895968	24.142110327878346
90-94	23.582827608270172	26.876633089522965	25.932630812391714	23.60790848981515
95-99	23.418671300760437	26.512485746492548	26.191254048800662	23.877588903946357
100-104	23.615923809044233	26.750023020268284	25.931328025163374	23.702725145524113
105-109	23.84791927296411	26.195753386291244	26.314121752472964	23.64220558827168
110-114	23.90127800349493	26.647340410109916	25.73526915937568	23.71611242701948
115-119	23.7681550013083	26.464429438732555	25.907843706937832	23.859571853021304
120-124	23.766677809758	26.58498472488305	25.666405071793296	23.98193239356565
125-129	23.76915554299122	27.038034902001385	24.93491086045466	24.257898694552733
130-134	23.722916500490456	26.625299196389317	25.601581980724657	24.050202322395567
135-139	24.118666983495014	26.300532996528315	25.55267264662428	24.02812737335239
140-144	24.248205992607204	26.439624667682576	25.46107939516643	23.85108994454379
145-149	24.464456674994185	26.19965422686351	25.364630347723143	23.971258750419164
150-151	25.819348931071296	12.879805507416394	30.13982231341683	31.161023248095475
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6292.0
1	3263.5
2	232.0
3	245.0
4	271.5
5	283.5
6	296.0
7	324.5
8	383.5
9	474.0
10	560.5
11	677.5
12	853.0
13	1077.5
14	1295.5
15	1472.5
16	1723.0
17	2121.0
18	2694.5
19	3429.0
20	4280.5
21	5421.5
22	7178.5
23	9464.0
24	13365.0
25	18948.5
26	25043.5
27	31472.0
28	42633.5
29	58823.5
30	77028.5
31	102259.0
32	136200.0
33	176047.0
34	235994.0
35	334160.0
36	434145.0
37	521732.5
38	616945.0
39	731970.5
40	792425.0
41	838612.0
42	940598.0
43	1039023.0
44	1164076.5
45	1252538.5
46	1249657.5
47	1264501.0
48	1324379.0
49	1299686.0
50	1188629.5
51	1062656.5
52	992389.0
53	950912.5
54	821295.0
55	698876.5
56	624765.0
57	573191.5
58	515038.5
59	422530.0
60	350311.0
61	307702.0
62	250238.5
63	188759.5
64	149620.0
65	125361.5
66	111474.5
67	89397.5
68	67398.0
69	60964.0
70	66586.5
71	60923.5
72	49153.5
73	37809.5
74	26895.0
75	20011.0
76	13979.0
77	11432.0
78	10700.0
79	11219.5
80	10102.5
81	8058.5
82	6472.0
83	4843.0
84	4099.5
85	3567.0
86	3098.5
87	2544.5
88	2084.0
89	1786.5
90	1552.5
91	1407.5
92	1261.0
93	1114.0
94	1084.0
95	1083.5
96	1078.5
97	1112.5
98	1233.0
99	1500.0
100	4106.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03841300501365861
2	0.02095217998056263
3	0.01673582209156868
4	0.015771851700040765
5	0.015715147559362652
6	0.019757342730559544
7	0.016849230372924906
8	0.017250209653434417
9	0.01751347887801137
10-14	0.025814965073287063
15-19	0.02945375078651681
20-24	0.030606464960587585
25-29	0.03463326900788613
30-34	0.029066542511600554
35-39	0.022031695665559996
40-44	0.0124020310843658
45-49	0.008539241143186837
50-54	0.010513513888436927
55-59	0.00839021205349338
60-64	0.011105543392277048
65-69	0.011104654062470982
70-74	0.013999730444604223
75-79	0.012280849532933784
80-84	0.012204998910094246
85-89	0.013298440931094844
90-94	0.015085340418078392
95-99	0.013580532708985094
100-104	0.013309102386283939
105-109	0.01841101719422068
110-114	0.01712611366998134
115-119	0.015601104342328203
120-124	0.015472152025773142
125-129	0.02187405989916431
130-134	0.013675423462310324
135-139	0.015048517693780692
140-144	0.009377483511353037
145-149	0.007442586390892188
150-151	0.0056406559611118815
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
35-39	4540.0
40-44	910.0
45-49	1081.0
50-54	1296.0
55-59	1469.0
60-64	1683.0
65-69	1649.0
70-74	1920.0
75-79	2119.0
80-84	2688.0
85-89	2709.0
90-94	2984.0
95-99	3223.0
100-104	3684.0
105-109	4383.0
110-114	5096.0
115-119	6188.0
120-124	7337.0
125-129	9355.0
130-134	11370.0
135-139	14015.0
140-144	18121.0
145-149	2097312.0
150-152	2.2484423E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.30812952574564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.841162534739226	20.302804187207894
2	16.925248999165486	10.936462738410707
3	8.034160522052765	7.78706096331339
4	4.012089133477806	5.184923815729504
5	2.2224006505835874	3.590080403857797
6	1.363426821525098	2.6429862209225137
7	0.9019559481111834	2.0398356718664985
8	0.6279279581712381	1.6229742244346683
9	0.46337835520874304	1.347379912755994
>10	2.2943937861980013	13.2783683087362
>50	0.16398520074935197	3.623117028498715
>100	0.11621843986615614	7.705112086342211
>500	0.017113779713339508	3.9001444414473854
>1k	0.014586445336872512	9.542324252353607
>5k	0.0013431887059020462	2.959580911937016
>10k+	6.082363951254549E-4	3.5368448321859627
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA	80890	0.32762842424660954	No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAG	72495	0.29362619131855555	No Hit
CCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTC	37409	0.15151751418767978	No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGGTCCCGAAGGCCAACACAATA	32230	0.1305410324325408	No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGT	31379	0.1270942307384641	No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCGGCGGTGTCCTAAG	31033	0.12569282840456217	No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA	25308	0.10250488516297682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	8.100591525444667E-6	0.0	0.0	4.050295762722333E-6
2	0.0	1.2150887288167E-5	0.0	0.0	2.0251478813611667E-5
3	4.050295762722333E-6	1.2150887288167E-5	0.0	0.0	3.6452661864501E-5
4	2.0251478813611667E-5	1.2150887288167E-5	1.6201183050889333E-5	0.0	3.6452661864501E-5
5	2.8352070339056335E-5	1.2150887288167E-5	1.6201183050889333E-5	0.0	3.6452661864501E-5
6	3.2402366101778666E-5	1.2150887288167E-5	2.8352070339056335E-5	0.0	3.6452661864501E-5
7	3.2402366101778666E-5	1.2150887288167E-5	2.8352070339056335E-5	0.0	3.6452661864501E-5
8	4.455325338994567E-5	1.2150887288167E-5	2.8352070339056335E-5	0.0	3.6452661864501E-5
9	4.455325338994567E-5	1.2150887288167E-5	2.8352070339056335E-5	0.0	3.6452661864501E-5
10-14	7.614556033917988E-5	2.1061537966156133E-5	2.8352070339056335E-5	0.0	6.318461389846839E-5
15-19	1.3771005593255935E-4	3.15923069492342E-5	3.15923069492342E-5	1.1340828135622533E-5	9.396686169515814E-5
20-24	2.405875683057066E-4	3.807278016958993E-5	3.564260271195654E-5	2.0251478813611667E-5	9.7207098305336E-5
25-29	6.148348967812501E-4	4.455325338994567E-5	3.9692898474678865E-5	2.3491715423789535E-5	1.0125739406805835E-4
30-34	0.0013811508550883157	4.455325338994567E-5	6.31846138984684E-5	2.91621294916008E-5	1.1583845881385873E-4
35-39	0.002580848460006671	4.698343084757907E-5	7.857573779681325E-5	5.184378576284587E-5	1.3446981932238147E-4
40-44	0.0038907141096710734	5.7514199830657134E-5	1.1097810389859193E-4	6.885502796627967E-5	1.5877159389871546E-4
45-49	0.00533018922374259	7.695561949172433E-5	1.3933017423764824E-4	7.04751462713686E-5	1.7902307271232715E-4
50-54	0.007011872024424904	8.667632932225792E-5	1.6444200796652674E-4	7.452544203409093E-5	2.0575502474629454E-4
55-59	0.008809393283921076	1.020674532206028E-4	1.7983313186487163E-4	8.343609271208006E-5	2.1061537966156132E-4
60-64	0.010593953596976536	1.1502839966131427E-4	2.017047289835722E-4	8.5056211017169E-5	2.1223549796665027E-4
65-69	0.012168708589522978	1.2312899118675894E-4	2.0656508389883902E-4	8.910650677989134E-5	2.235763261022728E-4
70-74	0.013817989024103514	1.3852011508510382E-4	2.0656508389883902E-4	8.910650677989134E-5	2.2924674017008408E-4
75-79	0.015570146971057195	1.620118305088933E-4	2.0737514305138347E-4	9.234674339006922E-5	2.6002898796677375E-4
80-84	0.01736847828970591	1.7821301355978267E-4	2.1466567542428367E-4	9.315680254261366E-5	2.7542011186511864E-4
85-89	0.019356363450050033	2.1547573457682815E-4	2.1466567542428367E-4	9.47769208477026E-5	2.8352070339056335E-4
90-94	0.021545143280225183	2.2681656271245068E-4	2.2600650355990623E-4	9.801715745788047E-5	2.932414132210969E-4
95-99	0.023788197073620808	2.2843668101753964E-4	2.4139762745825107E-4	1.0287751237314726E-4	3.0053194559399713E-4
100-104	0.026040971576846973	2.3491715423789536E-4	2.511183372887847E-4	1.1988875457658106E-4	3.0782247796689737E-4
105-109	0.02842902595854806	2.4625798237351787E-4	2.6731952033967404E-4	1.3771005593255935E-4	3.175431877974309E-4
110-114	0.030920767911774838	2.5678875135659595E-4	2.746100527125742E-4	1.4419052915291508E-4	3.2564377932287563E-4
115-119	0.033623125244663175	2.6488934288204055E-4	2.79470407627841E-4	1.506710023732708E-4	3.4670531728903174E-4
120-124	0.03644861156873828	2.746100527125742E-4	2.79470407627841E-4	1.5796153474617098E-4	3.5480590881447645E-4
125-129	0.03911289612145703	2.867609400007412E-4	2.79470407627841E-4	1.6444200796652674E-4	3.7262721017045466E-4
130-134	0.040810780105190236	2.9567159067873033E-4	2.8190058508547445E-4	1.774029544072382E-4	4.00979280509511E-4
135-139	0.0420582712001087	2.9567159067873033E-4	2.8352070339056335E-4	1.8145325016996052E-4	4.0745975372986676E-4
140	0.04218788066451582	2.9567159067873033E-4	2.8352070339056335E-4	1.82263309322505E-4	4.0907987203495566E-4
>>END_MODULE
