FastQCFastQC Report
Tue 19 Dec 2023
SRR12391891_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR12391891_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29952310
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGACTGTCCCTGTTAATCATTACTCCGGTCCCGAAGGCCAACACAATA1499740.500709294208026No Hit
CTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAG1190390.39742844541873396No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCGGCGGTGTCCTAAG1145190.3823377896395971No Hit
CCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAA1141310.3810423970638659No Hit
CCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGC1074470.3587269228984342No Hit
CGCGTGTTTAGCTTGGATTCTGACTTAGAGGCGTTCAGTCATAATCCGAC700610.23390850321728107No Hit
CTTACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTT651900.2176459845667997No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT637850.21295519444076266No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA605450.20213799870527513No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAGGGAT594740.1985623145593779No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGA589400.19677948044741791No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGT583210.19471286187943435No Hit
CACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCTT569530.19014560145778406No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGTA518030.17295160206341348No Hit
CTCCTACTCATCGGGGCATGGCAATCGCCCCGACGGCCGGGTATGGGTCG517690.17283808828100405No Hit
CTCGATTCCGTGGCGCGGCTCAACGGAGCAGCCGCGCCGTCCTACCTATT516090.17230390577554786No Hit
CCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCA514650.17182314152063732No Hit
GTCTGACATGTGTGCGAGTCGACGGGCGAGTAAACCCGTAAGGCGCAAGG513120.17131232949979483No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGTGGTGCGG510450.17042091244381485No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGA470880.15720991135575185No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC461910.1542151506845382No Hit
CCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGC438780.1464928748400374No Hit
CCCCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG427420.14270017905129853No Hit
CCCTCTTCGGCCTTCAAAGTTCTCATTTGAATATTTGCTACTACCACCAA424370.14168189365027273No Hit
CTGACATGTGTGCGAGTCGACGGGCGAGTAAACCCGTAAGGCGCAAGGAA423620.14143149560084015No Hit
GTTAATCATTACTCCGGTCCCGAAGGCCAACACAATAGGACCGAAATCCT414440.13836662347578532No Hit
CTGTGATGTTATCCCATGCTAATGTATCCAGAGCGTAGGCTTGCTTTGAG403440.13469411875077414No Hit
CCCCGACTGTCCCTGTTAATCATTACTCCGGTCCCGAAGGCCAACACAAT392520.13104832315103576No Hit
CTCCAATGGATCCTCGTTAAGGGATTTAGATTGTACTCATTCCAATTACC391210.1306109612246935No Hit
CGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC383190.12793337141609445No Hit
CCGAAGGCCAACACAATAGGACCGAAATCCTGTGATGTTATCCCATGCTA374710.12510220413717674No Hit
CTCCGTCACCCGTCACCACCACAGTAGGCCCCTATCCTACCGTCGAAAGT368000.12286197625491989No Hit
CCCCACCTGACAATGTCTTCCGCCCGGATCGGCCCGCCGAGGCGGGCCTT364650.12174353163412104No Hit
CCTCGCGGTACTTGTTCGCTATCGGTCTCTCGCCGGTATTTAGCCTTGGA362880.12115259223746015No Hit
GCGGTTATGAGTACGACCGGGCGTGGGAGGCACTCGGTCCTCCGGATTTT357990.1195199969551597No Hit
GGCGTGGGCACCCGCTGAATTTAAGCATATCAATAAGCGGAGGAGAAGAA354630.11839821369370176No Hit
CCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAAAATGGCC339980.11350710512811867No Hit
CCGACTGTCCCTGTTAATCATTACTCCGGTCCCGAAGGCCAACACAATAG334820.11178436654802251No Hit
ACCAGGTCCAGACATAGTAAGGATTGACAGACTGAGAGCTCTTTCTTGAT332850.1111266543381796No Hit
CTTTTGTTCCACACGAGATTTCTGTTCTCGTTGAGCTCATCTTAGGACAC329340.10995479146683511No Hit
CTGCAAAGGATTCTGCCCGCCGTCCGGTCGAAATTAATCTTCAAGGCGGC326450.1089899243163549No Hit
CAAAAGGGTAAAAGCTCGTTTGATTCTGATTTCCAGTACGAATACGAACC320580.10703014224946257No Hit
CCGGAATCGAACCCTAATTCTCCGTCACCCGTCACCACCACAGTAGGCCC317040.10584826345614079No Hit
CGGGCGGCGGGCGCGAGCGCCGCGTGTTTAGCTTGGATTCTGACTTAGAG316900.10580152248691335No Hit
GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTAATCGTCTTCGAG315630.10537751512320753No Hit
GCGGCGGGCGCGAGCGCCGCGTGTTTAGCTTGGATTCTGACTTAGAGGCG314030.10484333261775135No Hit
CCCGCATCGCCAGTTCTGCTTACCAAAAATGGCCCACTTGGAGCTCTCGA312420.10430581147163606No Hit
CCACGCTTTCACGGTTCGTATTCGTACTGGAAATCAGAATCAAACGAGCT308060.10285016414426801No Hit
CCCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCGGCGGTGTCCTAA307680.10272329579922217No Hit
CGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA305360.10194873116631072No Hit
CCAAGATCCGCACCGACGGCCGCTCCGCCCGGGCTCGCGCCCCGGGTTTT300110.10019594482028264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)