##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654119_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32083105
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6698154682971	32.0	32.0	32.0	32.0	32.0
2	31.70146031688641	32.0	32.0	32.0	32.0	32.0
3	35.833193670001705	37.0	37.0	37.0	32.0	37.0
4	36.39087550908804	37.0	37.0	37.0	37.0	37.0
5	36.56355845233808	37.0	37.0	37.0	37.0	37.0
6	40.18732105262256	41.0	41.0	41.0	37.0	41.0
7	40.225897867428976	41.0	41.0	41.0	37.0	41.0
8	40.25560100869289	41.0	41.0	41.0	41.0	41.0
9	40.299815120762155	41.0	41.0	41.0	41.0	41.0
10-11	40.32927010961065	41.0	41.0	41.0	41.0	41.0
12-13	40.307569279843705	41.0	41.0	41.0	41.0	41.0
14-15	40.304214242979285	41.0	41.0	41.0	41.0	41.0
16-17	40.301663632619096	41.0	41.0	41.0	41.0	41.0
18-19	40.300302028123525	41.0	41.0	41.0	41.0	41.0
20-21	40.26987102713406	41.0	41.0	41.0	41.0	41.0
22-23	40.276675776861374	41.0	41.0	41.0	41.0	41.0
24-25	40.265395712166885	41.0	41.0	41.0	41.0	41.0
26-27	40.18503701558811	41.0	41.0	41.0	41.0	41.0
28-29	40.19681235653469	41.0	41.0	41.0	41.0	41.0
30-31	40.111865840291955	41.0	41.0	41.0	41.0	41.0
32-33	40.12875889038795	41.0	41.0	41.0	41.0	41.0
34-35	40.146462787813086	41.0	41.0	41.0	41.0	41.0
36-37	40.13423795795326	41.0	41.0	41.0	41.0	41.0
38-39	40.06356680564428	41.0	41.0	41.0	37.0	41.0
40-41	40.08533400990957	41.0	41.0	41.0	37.0	41.0
42-43	40.074084553225134	41.0	41.0	41.0	37.0	41.0
44-45	40.0710764590896	41.0	41.0	41.0	37.0	41.0
46-47	40.062869881203824	41.0	41.0	41.0	37.0	41.0
48-49	39.97313288411455	41.0	41.0	41.0	37.0	41.0
50-51	39.934947973395964	41.0	41.0	41.0	37.0	41.0
52-53	39.97801070064759	41.0	41.0	41.0	37.0	41.0
54-55	39.94824255632365	41.0	41.0	41.0	37.0	41.0
56-57	39.94105539348514	41.0	41.0	41.0	37.0	41.0
58-59	39.94042696303865	41.0	41.0	41.0	37.0	41.0
60-61	39.92700834286457	41.0	41.0	41.0	37.0	41.0
62-63	39.88445005556663	41.0	41.0	41.0	37.0	41.0
64-65	39.832959902104236	41.0	41.0	41.0	37.0	41.0
66-67	39.81234927230391	41.0	41.0	41.0	37.0	41.0
68-69	39.790661549123755	41.0	41.0	41.0	37.0	41.0
70-71	39.77105411087861	41.0	41.0	41.0	37.0	41.0
72-73	39.74898651486507	41.0	41.0	41.0	37.0	41.0
74-75	39.72595535874723	41.0	41.0	41.0	37.0	41.0
76-77	39.19906961935261	41.0	39.0	41.0	34.5	41.0
78-79	39.52708370963471	41.0	41.0	41.0	37.0	41.0
80-81	39.707306618234114	41.0	41.0	41.0	37.0	41.0
82-83	39.780167864051805	41.0	41.0	41.0	37.0	41.0
84-85	39.820440711707924	41.0	41.0	41.0	37.0	41.0
86-87	39.83257585261776	41.0	41.0	41.0	37.0	41.0
88-89	39.715565186100285	41.0	41.0	41.0	37.0	41.0
90-91	39.738295405011456	41.0	41.0	41.0	37.0	41.0
92-93	39.693967494729705	41.0	41.0	41.0	37.0	41.0
94-95	39.69220466659945	41.0	41.0	41.0	37.0	41.0
96-97	39.66183832581042	41.0	41.0	41.0	37.0	41.0
98-99	39.62207997012758	41.0	41.0	41.0	37.0	41.0
100-101	39.114368637324844	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	2.0
16	6.0
17	27.0
18	65.0
19	179.0
20	522.0
21	1407.0
22	3481.0
23	6969.0
24	13997.0
25	24802.0
26	41882.0
27	65831.0
28	95908.0
29	132735.0
30	173539.0
31	219956.0
32	273568.0
33	330925.0
34	394260.0
35	479706.0
36	607061.0
37	817206.0
38	1267545.0
39	2985902.0
40	2.4145623E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.536820935832026	10.776850215922108	8.523667381494992	45.162661466750876
2	22.626709603076137	13.14392419312283	35.11508627360101	29.11427993020002
3	19.8019036949131	16.864670295702886	24.029066160165037	39.304359849218976
4	23.270447171494155	24.857668857175764	22.385732303653278	29.4861516676768
5	23.91663462747761	29.763204029036466	24.533972008008575	21.78618933547735
6	20.370250946721026	32.58334565809638	24.891290914641832	22.15511248054077
7	16.503162645884807	23.705289746737417	40.359958925421964	19.431588681955812
8	19.633782328736572	23.78024196847531	30.45213360739243	26.133842095395693
9	19.288158050787167	22.36794412510884	33.271891857100485	25.07200596700351
10-11	22.132036182905367	30.00529206479687	23.886362598216333	23.976309154081434
12-13	22.275415674386878	23.629918301236742	27.108771111773628	26.985894912602753
14-15	21.3479243982152	25.54674337162815	27.502595213275022	25.602737016881626
16-17	21.9561789530201	25.8884740909416	26.882196920622786	25.273150035415515
18-19	21.81362130104257	26.34950265387788	26.49770189255284	25.33917415252671
20-21	22.030705880867828	26.048974997899986	26.687233046801424	25.233086074430766
22-23	22.004029846861766	26.28805721890073	26.63018744600936	25.077725488228147
24-25	21.90120512503467	25.965622482218887	26.539130726959005	25.594041665787437
26-27	21.808810275688714	26.089951393420307	26.527778405487872	25.573459925403107
28-29	21.85603210871701	26.228017135928148	26.50746730332365	25.408483452031188
30-31	22.083123490662025	26.043213343196424	26.295518586534573	25.578144579606978
32-33	22.001975806269375	26.030842401319948	26.50259381066764	25.464587981743037
34-35	22.21985683742269	26.205195849965268	26.2069756028913	25.367971709720738
36-37	21.98114587071834	26.007919526615638	26.27301076801966	25.737923834646363
38-39	21.998221911077007	26.05907996171397	26.532878553596582	25.409819573612435
40-41	22.311676192189005	26.340868503843378	26.1237402053199	25.223715098647716
42-43	22.25011419561791	26.01551190260419	26.27973508175097	25.454638820026926
44-45	22.227144162012998	26.066677461548686	26.477898569979434	25.228279806458882
46-47	22.146720286719194	26.05243800257403	26.40275935711232	25.398082353594457
48-49	22.128467615587706	25.819550507969847	26.2193668599096	25.832615016532845
50-51	22.26622699779373	25.878404478635463	26.373292226145185	25.48207629742563
52-53	22.59594757504162	26.251747695498075	25.99137118351569	25.16093354594462
54-55	22.331234772943578	26.09085373750452	26.032227865725588	25.54568362382631
56-57	22.195102546457967	25.81788162992448	26.48410106973661	25.50291475388094
58-59	22.23449055261503	25.89858329589405	26.439685082388344	25.42724106910258
60-61	22.410433099029017	25.68701656695689	26.379981808308493	25.522568525705598
62-63	22.241311467070567	25.840488527293886	26.35242890916966	25.565771096465888
64-65	22.335802241173937	26.085636418540314	26.11182673205481	25.466734608230933
66-67	22.407571652433976	25.843459857415922	26.089770772991468	25.659197717158634
68-69	22.320533486885235	25.98412590507928	26.461330893907775	25.234009714127716
70-71	22.6368377427904	26.18690659968798	26.159692676856793	25.01656298066482
72-73	22.677744251998053	25.71448742258581	26.078322219747747	25.52944610566839
74-75	22.461847131067895	25.714258330046295	26.39443719677382	25.429457342111995
76-77	22.47645850571921	26.00914838562035	26.240575991779274	25.27381711688116
78-79	22.43108206688664	25.850919445778697	26.063352129779087	25.654646357555578
80-81	22.502497304768557	25.974996156793832	26.21228842261728	25.310218115820334
82-83	22.783660489054878	26.1314710163881	25.826980375550303	25.257888119006722
84-85	22.61952142587731	25.979170589422836	25.81457613022832	25.586731854471527
86-87	22.632760314958805	25.859141841084377	26.162835730136884	25.345262113819935
88-89	22.849652488435893	25.92813569634236	25.999693608209057	25.222518207012694
90-91	22.929868308267746	25.69965869243357	26.00998051897452	25.360492480324165
92-93	22.789386284454455	25.710927926252502	26.17564620860907	25.324039580683973
94-95	22.91757453027068	25.849097211756778	26.095756629540688	25.13757162843185
96-97	22.985844118281303	25.871476051143492	25.74218026109103	25.400499569484168
98-99	22.974119016458197	26.109132521918426	25.864498054710968	25.052250406912407
100-101	23.013374173104506	26.388748844602166	25.521580595145014	25.07629638714831
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	43.0
1	34.0
2	24.0
3	29.5
4	36.0
5	41.5
6	52.0
7	62.0
8	67.0
9	75.0
10	98.0
11	118.5
12	140.5
13	172.5
14	204.5
15	234.5
16	321.0
17	443.5
18	514.0
19	618.0
20	860.0
21	1166.5
22	1843.0
23	2811.5
24	4111.0
25	6346.0
26	10106.0
27	15425.0
28	22058.0
29	30733.5
30	42641.5
31	58418.0
32	82123.0
33	115850.0
34	152109.0
35	195472.0
36	260831.0
37	348490.5
38	461457.0
39	632800.5
40	843891.5
41	1059212.0
42	1286163.0
43	1511086.5
44	1737207.0
45	1928508.5
46	2034569.5
47	2059483.5
48	2011044.0
49	1919876.0
50	1812002.0
51	1673113.5
52	1511715.5
53	1333693.5
54	1164194.5
55	1015403.0
56	858391.5
57	722142.5
58	595726.0
59	462334.0
60	373675.5
61	338313.5
62	303460.5
63	256462.0
64	203977.0
65	158333.5
66	130031.5
67	101580.0
68	73622.5
69	52815.5
70	35864.5
71	22801.0
72	16841.5
73	12910.0
74	7949.0
75	3541.0
76	1497.0
77	557.0
78	84.5
79	23.0
80	10.5
81	7.0
82	3.5
83	1.5
84	0.5
85	1.0
86	2.5
87	3.0
88	1.5
89	1.5
90	1.5
91	1.0
92	1.0
93	0.5
94	1.0
95	1.0
96	0.5
97	0.5
98	1.0
99	0.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059208733069944446
2	0.0
3	7.168882188927786E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.127309217733134E-4
12-13	0.0
14-15	0.0
16-17	0.0012981910572558361
18-19	0.0019293643804114346
20-21	0.0
22-23	0.0
24-25	1.3714383317948807E-4
26-27	0.0
28-29	3.241581511515173E-4
30-31	0.0016753365984994283
32-33	0.0
34-35	0.0
36-37	1.55845264976691E-6
38-39	0.0028285915593269416
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0021958597835215763
48-49	0.0
50-51	0.0014836469225780985
52-53	4.426005525338025E-4
54-55	0.0
56-57	6.467578496532676E-4
58-59	1.4649454907808952E-4
60-61	0.0010192280329475592
62-63	0.0032696336592109776
64-65	0.001973001054604908
66-67	8.727334838694696E-4
68-69	4.426005525338025E-4
70-71	8.181876411276278E-4
72-73	0.0
74-75	0.0
76-77	8.914349156666726E-4
78-79	1.55845264976691E-6
80-81	0.0016114400398589848
82-83	0.0010768907809889348
84-85	2.493524239627056E-5
86-87	9.53773021657349E-4
88-89	0.0
90-91	0.001885727706217961
92-93	0.0010426048226940628
94-95	0.0
96-97	2.509108766124725E-4
98-99	0.0010613062544912657
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.2083105E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.678177195657298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.9682443638762	15.655533820837473
2	15.641583597188319	8.032947104585766
3	7.023984862886541	5.410893837864457
4	3.8775356595525974	3.9827219099388587
5	2.4974615031205136	3.206512950823058
6	1.6946149119909584	2.610877319310421
7	1.2421139006124107	2.2326654585980177
8	0.9144751762212192	1.8785644492830718
9	0.7282379630540465	1.6829841110315706
>10	4.685289264424933	22.844536995910868
>50	0.4180246533602815	7.435400576941636
>100	0.2686772792215062	13.314218658810853
>500	0.025238139718332876	4.441910214636924
>1k	0.014004202967925834	6.339144579799201
>5k	4.6306962376237864E-4	0.7430088410303101
>10k+	5.1452180418042066E-5	0.1880791705976791
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.740286359440584E-5	0.0	0.0	0.0	3.11690529953382E-6
2	4.987048479254112E-5	3.11690529953382E-6	0.0	0.0	6.23381059906764E-6
3	9.039025368648078E-5	6.23381059906764E-6	0.0	0.0	1.246762119813528E-5
4	1.1220859078321752E-4	6.23381059906764E-6	6.23381059906764E-6	0.0	1.246762119813528E-5
5	1.4337764377855573E-4	6.23381059906764E-6	6.23381059906764E-6	0.0	1.246762119813528E-5
6	1.714297914743601E-4	6.23381059906764E-6	2.181833709673674E-5	0.0	1.246762119813528E-5
7	1.9013122327156303E-4	6.23381059906764E-6	2.181833709673674E-5	0.0	1.246762119813528E-5
8	2.5558623456177325E-4	9.35071589860146E-6	2.181833709673674E-5	0.0	1.246762119813528E-5
9	2.836383822575776E-4	9.35071589860146E-6	2.181833709673674E-5	0.0	1.246762119813528E-5
10-11	3.1636588790268273E-4	1.246762119813528E-5	2.493524239627056E-5	0.0	1.55845264976691E-5
12-13	3.724701832942915E-4	1.870143179720292E-5	2.649369504603747E-5	3.11690529953382E-6	2.025988444696983E-5
14-15	4.3169138398543405E-4	1.870143179720292E-5	2.805214769580438E-5	3.11690529953382E-6	2.181833709673674E-5
16-17	5.01821753224945E-4	1.870143179720292E-5	2.805214769580438E-5	3.11690529953382E-6	2.337678974650365E-5
18-19	5.516922380174861E-4	1.870143179720292E-5	2.805214769580438E-5	3.11690529953382E-6	2.493524239627056E-5
20-21	6.124718913583956E-4	2.025988444696983E-5	2.805214769580438E-5	3.11690529953382E-6	2.493524239627056E-5
22-23	6.997452397453426E-4	2.337678974650365E-5	2.805214769580438E-5	3.11690529953382E-6	2.805214769580438E-5
24-25	7.823432301829888E-4	2.961060034557129E-5	2.805214769580438E-5	3.11690529953382E-6	2.805214769580438E-5
26-27	8.898764630169056E-4	3.11690529953382E-5	2.805214769580438E-5	3.11690529953382E-6	2.805214769580438E-5
28-29	0.0010675400650903334	3.11690529953382E-5	2.805214769580438E-5	3.11690529953382E-6	2.805214769580438E-5
30-31	0.0013776721423939486	3.11690529953382E-5	3.2727505645105105E-5	3.11690529953382E-6	3.2727505645105105E-5
32-33	0.0018093635263793824	3.740286359440584E-5	3.428595829487202E-5	3.11690529953382E-6	3.428595829487202E-5
34-35	0.0024545629233828836	5.1428937442308034E-5	3.428595829487202E-5	3.11690529953382E-6	3.7402863594405844E-5
36-37	0.0032665167539114434	5.766274804137567E-5	3.428595829487202E-5	3.11690529953382E-6	4.0519768893939664E-5
38-39	0.004620812106558889	5.922120069114258E-5	3.428595829487202E-5	3.11690529953382E-6	4.5195126843240396E-5
40-41	0.006567319466117759	5.922120069114258E-5	3.428595829487202E-5	3.11690529953382E-6	4.67535794930073E-5
42-43	0.00920422134952337	5.922120069114258E-5	3.428595829487202E-5	6.23381059906764E-6	4.67535794930073E-5
44-45	0.01228060688016325	6.23381059906764E-5	3.428595829487202E-5	6.23381059906764E-6	5.298739009207494E-5
46-47	0.01572011187819882	6.857191658974404E-5	3.428595829487202E-5	6.23381059906764E-6	5.298739009207494E-5
48-49	0.020090013108145236	6.857191658974404E-5	3.428595829487202E-5	6.23381059906764E-6	5.298739009207494E-5
50-51	0.026454733729793296	7.168882188927786E-5	3.428595829487202E-5	6.23381059906764E-6	5.298739009207494E-5
52-53	0.034981028176668066	7.79226324883455E-5	3.428595829487202E-5	6.23381059906764E-6	5.298739009207494E-5
54-55	0.04476499391190472	8.103953778787933E-5	3.428595829487202E-5	7.79226324883455E-6	5.610429539160876E-5
56-57	0.05550273266879874	9.039025368648078E-5	5.1428937442308034E-5	9.35071589860146E-6	5.610429539160876E-5
58-59	0.068084120910367	9.194870633624769E-5	6.23381059906764E-5	9.35071589860146E-6	5.610429539160876E-5
60-61	0.08446657516471676	9.662406428554842E-5	6.23381059906764E-5	9.35071589860146E-6	5.610429539160876E-5
62-63	0.10593893577320523	9.662406428554842E-5	6.23381059906764E-5	9.35071589860146E-6	5.610429539160876E-5
64-65	0.13233912366025669	9.662406428554842E-5	6.23381059906764E-5	9.35071589860146E-6	5.610429539160876E-5
66-67	0.16339440961216192	9.662406428554842E-5	6.23381059906764E-5	9.35071589860146E-6	5.610429539160876E-5
68-69	0.19942115951682357	9.974096958508224E-5	6.23381059906764E-5	9.35071589860146E-6	5.610429539160876E-5
70-71	0.24334458899785416	9.974096958508224E-5	6.23381059906764E-5	9.35071589860146E-6	5.610429539160876E-5
72-73	0.2983283569342805	1.0597478018414988E-4	6.545501129021022E-5	9.35071589860146E-6	6.545501129021022E-5
74-75	0.36829820555086545	1.2623466463111972E-4	6.857191658974404E-5	9.35071589860146E-6	6.545501129021022E-5
76-77	0.4559377903105077	1.4649454907808955E-4	7.013036923951095E-5	9.35071589860146E-6	6.701346393997714E-5
78-79	0.5622788068673528	1.5116990702739025E-4	7.168882188927786E-5	9.35071589860146E-6	6.857191658974404E-5
80-81	0.6904319267103356	1.5272835967715718E-4	7.480572718881168E-5	9.35071589860146E-6	6.857191658974404E-5
82-83	0.8437353554152567	1.542868123269241E-4	7.480572718881168E-5	9.35071589860146E-6	7.79226324883455E-5
84-85	1.035342433346149	1.6052062292599173E-4	8.415644308741314E-5	9.35071589860146E-6	8.103953778787933E-5
86-87	1.2764132399279933	1.6519598087529245E-4	8.415644308741314E-5	9.35071589860146E-6	8.103953778787933E-5
88-89	1.565412699300769	1.714297914743601E-4	8.727334838694697E-5	9.35071589860146E-6	8.103953778787933E-5
90	1.8137053754616332	1.7454669677389393E-4	9.039025368648078E-5	9.35071589860146E-6	8.415644308741314E-5
>>END_MODULE
