##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654127_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32665390
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.613041356616286	32.0	32.0	32.0	32.0	32.0
2	31.672541028899396	32.0	32.0	32.0	32.0	32.0
3	35.68676051319149	37.0	37.0	37.0	32.0	37.0
4	36.33049306926995	37.0	37.0	37.0	37.0	37.0
5	36.41363060413484	37.0	37.0	37.0	37.0	37.0
6	40.05056314955983	41.0	41.0	41.0	37.0	41.0
7	40.065578093511206	41.0	41.0	41.0	37.0	41.0
8	40.205053789347076	41.0	41.0	41.0	37.0	41.0
9	40.18350468186665	41.0	41.0	41.0	37.0	41.0
10-11	40.25459823072677	41.0	41.0	41.0	41.0	41.0
12-13	40.21694865727916	41.0	41.0	41.0	41.0	41.0
14-15	40.24899003195737	41.0	41.0	41.0	41.0	41.0
16-17	40.24929841645852	41.0	41.0	41.0	41.0	41.0
18-19	40.24236359951618	41.0	41.0	41.0	41.0	41.0
20-21	40.173557364537814	41.0	41.0	41.0	39.0	41.0
22-23	40.13030155158104	41.0	41.0	41.0	39.0	41.0
24-25	40.16722731612879	41.0	41.0	41.0	39.0	41.0
26-27	40.137280161051194	41.0	41.0	41.0	41.0	41.0
28-29	40.05417129567411	41.0	41.0	41.0	37.0	41.0
30-31	40.068171097911275	41.0	41.0	41.0	37.0	41.0
32-33	40.077673295803294	41.0	41.0	41.0	37.0	41.0
34-35	40.06615717736724	41.0	41.0	41.0	37.0	41.0
36-37	40.04734657997348	41.0	41.0	41.0	37.0	41.0
38-39	40.020616943498915	41.0	41.0	41.0	37.0	41.0
40-41	40.01340732500056	41.0	41.0	41.0	37.0	41.0
42-43	39.956592849496054	41.0	41.0	41.0	37.0	41.0
44-45	39.97507732189942	41.0	41.0	41.0	37.0	41.0
46-47	39.960499951171556	41.0	41.0	41.0	37.0	41.0
48-49	39.93901396860714	41.0	41.0	41.0	37.0	41.0
50-51	39.86668115702889	41.0	41.0	41.0	37.0	41.0
52-53	39.888871815092365	41.0	41.0	41.0	37.0	41.0
54-55	39.83621942061613	41.0	41.0	41.0	37.0	41.0
56-57	39.83182085075366	41.0	41.0	41.0	37.0	41.0
58-59	39.82645388590187	41.0	41.0	41.0	37.0	41.0
60-61	39.74868120356132	41.0	41.0	41.0	37.0	41.0
62-63	39.71109619386145	41.0	41.0	41.0	37.0	41.0
64-65	39.70224166924075	41.0	41.0	41.0	37.0	41.0
66-67	39.66697954624145	41.0	41.0	41.0	37.0	41.0
68-69	39.584852959049314	41.0	41.0	41.0	37.0	41.0
70-71	39.60157025524569	41.0	41.0	41.0	37.0	41.0
72-73	39.51273047711966	41.0	41.0	41.0	37.0	41.0
74-75	39.55348422290381	41.0	41.0	41.0	37.0	41.0
76-77	39.00420898082037	41.0	39.0	41.0	34.5	41.0
78-79	39.32951190847561	41.0	41.0	41.0	37.0	41.0
80-81	39.54689022540371	41.0	41.0	41.0	37.0	41.0
82-83	39.68752868096784	41.0	41.0	41.0	37.0	41.0
84-85	39.64768392479012	41.0	41.0	41.0	37.0	41.0
86-87	39.66564290216648	41.0	41.0	41.0	37.0	41.0
88-89	39.60366426055222	41.0	41.0	41.0	37.0	41.0
90-91	39.61998575556575	41.0	41.0	41.0	37.0	41.0
92-93	39.57219088766428	41.0	41.0	41.0	37.0	41.0
94-95	39.43101623767541	41.0	41.0	41.0	37.0	41.0
96-97	39.392860057694094	41.0	41.0	41.0	37.0	41.0
98-99	39.44208464983886	41.0	41.0	41.0	37.0	41.0
100-101	38.892382656383404	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	2.0
16	9.0
17	23.0
18	67.0
19	211.0
20	729.0
21	2024.0
22	4895.0
23	9886.0
24	18247.0
25	30696.0
26	50205.0
27	76684.0
28	110362.0
29	151404.0
30	197703.0
31	250919.0
32	308606.0
33	374333.0
34	449610.0
35	548771.0
36	695692.0
37	943400.0
38	1481350.0
39	3357795.0
40	2.3601766E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.868328287061463	11.352522334614527	6.335138803355253	51.44401057496876
2	21.830689913697647	13.122537339979715	34.97348110645549	30.07329163986715
3	20.015882253357454	14.583367901010824	20.954903645724112	44.445846199907606
4	23.350419306713082	21.941239863330175	20.194148971675563	34.51419185828118
5	24.718057858791827	27.15850935806981	23.873252393435376	24.250180389702987
6	20.359025255782957	31.185309589140065	25.275387803421296	23.180277351655683
7	15.81846106842747	26.04620976513674	39.25669033799994	18.878638828435847
8	17.952034247869076	24.532023037226864	32.7478961677788	24.768046547125262
9	18.25403754915046	22.429335657242998	35.072219395163444	24.244407398443094
10-11	20.625448731819436	31.440586651912934	25.46086587071454	22.473098745553095
12-13	21.214565040349495	25.508972244963285	27.62688824554691	25.649574469140312
14-15	20.837208739892592	26.25509445930387	27.39485890111828	25.512837899685266
16-17	21.537395334280756	26.47878320500508	26.67034224835114	25.31347921236302
18-19	21.24039956137646	26.674164821555134	26.5201561549763	25.56527946209211
20-21	21.53428146892856	26.655743136566368	26.737375722152805	25.072599672352265
22-23	21.400125637563182	26.824995813611903	26.775810728113147	24.99906782071177
24-25	21.32891450750955	26.503806115644206	26.668126825795234	25.49915255105101
26-27	21.22447795663851	26.676335411883954	26.888731467770626	25.210455163706907
28-29	21.260750583822556	26.897837734264897	26.624662688890314	25.216748993022232
30-31	21.38705369811902	26.45609772300285	26.660121002688165	25.496727576189965
32-33	21.52333952176694	26.48885413033476	26.73950543738331	25.248300910514992
34-35	21.6173008364995	26.626775453685042	26.44836371900935	25.307559990806112
36-37	21.464392348950195	26.368685345894445	26.391541425848963	25.775380879306397
38-39	21.55822929877572	26.504052506022997	26.692619510674536	25.245098684526745
40-41	21.641759860167504	26.782534640356726	26.460041717576853	25.115663781898924
42-43	21.627485543567673	26.332780658672682	26.523943231658954	25.515790566100694
44-45	21.652014736512086	26.381294675396344	26.724392596304963	25.24229799178661
46-47	21.652312987549777	26.50304481188512	26.465994813428807	25.3786473871363
48-49	21.588243621014982	26.262057622864287	26.458829554166897	25.69086920195383
50-51	21.59598786237907	26.311471043655395	26.67896868182767	25.413572412137864
52-53	21.99451927560026	26.47826032384735	26.241466885899722	25.285753514652665
54-55	21.81285266527257	26.247228597316845	26.306513597045534	25.633405140365056
56-57	21.71533539988975	26.232729719138277	26.724406036976678	25.327528843995296
58-59	21.79727924639909	26.336393282367894	26.534149967391844	25.332177503841173
60-61	21.7547242007108	26.0798585241672	26.534630699235677	25.630786575886322
62-63	21.80706626557219	26.092540497423443	26.697425719926304	25.402967517078068
64-65	21.919704975156773	26.257005320034306	26.512094623179514	25.311195081629407
66-67	21.781187276030174	26.068729157447677	26.477355345314642	25.672728221207514
68-69	21.857610126882392	26.12681020006682	26.663742432955917	25.351837240094877
70-71	22.054991348063254	26.297766784174048	26.454644848092297	25.192597019670398
72-73	22.03486735686572	26.10782385125488	26.31181332432231	25.54549546755709
74-75	21.979652232539888	26.138111391198876	26.61334541069919	25.26889096556204
76-77	21.92939683254968	26.248563081597986	26.469589066593112	25.35245101925922
78-79	21.86871593021239	26.006159876991042	26.504492370381964	25.6206318224146
80-81	21.971418544225703	26.062796576032564	26.629601209971888	25.33618366976984
82-83	22.07335833528607	26.31201317724431	26.355615253709775	25.25901323375985
84-85	22.154013863894896	26.01328964728881	26.33524240509091	25.49745408372538
86-87	22.092579245503746	26.04785014526147	26.541274687568666	25.318295921666117
88-89	22.277050482756458	26.100545985572715	26.44027313115936	25.182130400511465
90-91	22.146192356146244	25.86719820561978	26.418633795820064	25.567975642413916
92-93	22.131429437532578	25.92679393640252	26.522267774999314	25.41950885106558
94-95	22.34800699222228	26.11126357017961	26.250119370704894	25.290610066893215
96-97	22.250504568230202	26.02997447700765	26.13810225403862	25.58141870072353
98-99	22.278939111622083	26.140055805455738	26.423794510774673	25.15721057214751
100-101	22.331138057609635	26.250329220936962	26.286551943975766	25.13198077747763
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	28.0
1	28.5
2	26.5
3	25.5
4	36.5
5	64.0
6	93.5
7	112.5
8	128.0
9	165.5
10	217.5
11	270.0
12	303.5
13	341.0
14	390.5
15	488.0
16	638.5
17	763.5
18	920.5
19	1199.5
20	1569.5
21	2202.0
22	3136.0
23	4584.5
24	7217.0
25	10594.0
26	15203.5
27	22456.0
28	32537.0
29	46427.5
30	65567.5
31	90090.0
32	123507.0
33	165617.0
34	217360.0
35	285488.0
36	368033.5
37	463937.5
38	591144.5
39	763725.0
40	962409.5
41	1175961.5
42	1407196.0
43	1636915.5
44	1837010.5
45	1974075.0
46	2049267.0
47	2060634.0
48	1988633.0
49	1862771.0
50	1714412.0
51	1543226.0
52	1390786.5
53	1249963.5
54	1085738.5
55	925516.0
56	778692.5
57	637379.0
58	511702.5
59	414707.0
60	339660.5
61	292356.0
62	256768.5
63	218263.5
64	194226.5
65	176702.5
66	156849.5
67	135124.0
68	99320.0
69	71597.0
70	60187.5
71	45412.0
72	32775.0
73	29745.0
74	26449.0
75	19626.5
76	11930.5
77	3935.0
78	553.0
79	161.0
80	58.5
81	29.0
82	11.5
83	5.5
84	4.0
85	1.5
86	1.0
87	2.0
88	1.5
89	1.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	0.5
97	0.0
98	1.5
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033429877922780044
2	0.0
3	0.0
4	0.019108910072709984
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0027429643423819523
10-11	0.003410337363184704
12-13	0.0016653712078747568
14-15	0.0
16-17	8.127868670785807E-4
18-19	6.275755470851565E-5
20-21	3.8419868858140065E-4
22-23	0.0
24-25	1.530672066061357E-6
26-27	0.0
28-29	2.173554333807127E-4
30-31	0.0
32-33	0.00210926610703255
34-35	6.673730208027519E-4
36-37	3.704226399868485E-4
38-39	0.004298127161500291
40-41	6.734957090669972E-5
42-43	0.0
44-45	0.0024750967308212147
46-47	2.188861054467741E-4
48-49	4.959377494038798E-4
50-51	3.061344132122715E-5
52-53	0.0
54-55	9.107498793065077E-4
56-57	3.030730690801487E-4
58-59	7.622746888985559E-4
60-61	7.041091503882244E-5
62-63	3.122571014765169E-4
64-65	0.0022990694432241587
66-67	0.002126103499759225
68-69	0.008271751844995574
70-71	0.008452371148790815
72-73	6.857410855954881E-4
74-75	0.0013179086488788285
76-77	0.0
78-79	0.001383727547719467
80-81	0.008611561043661196
82-83	0.005534910190877868
84-85	1.1786174908672452E-4
86-87	0.009958552461795191
88-89	1.7755795966311745E-4
90-91	0.0022684560019029312
92-93	6.949251179918562E-4
94-95	0.013864827574383773
96-97	0.0010194275959968639
98-99	3.8266801651533935E-4
100-101	0.015159776142271682
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.266539E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.961869056048947
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.571905236464694	18.75419398406826
2	16.737404526360493	10.364426545665891
3	7.063109840336075	6.56061246014929
4	3.9120376824307397	4.844959938629991
5	2.4489073140039634	3.7911373793295637
6	1.6239137174228906	3.0167642326301527
7	1.2018936279031318	2.604901118850547
8	0.9485254566178806	2.3494496787305486
9	0.7010604998004466	1.9535529055670693
>10	4.288403341836293	24.56547728376866
>50	0.31529300590880144	6.699254731648176
>100	0.17271707851157025	9.79759212339577
>500	0.010331849863611164	2.1587370945053967
>1k	0.0044135714840152175	2.3837040642663148
>5k	8.32510555973539E-5	0.15523645879447853
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4490753056981716E-5	0.0	3.0613441321227146E-6	0.0	0.0
2	2.4490753056981716E-5	3.0613441321227146E-6	6.122688264245429E-6	0.0	0.0
3	4.592016198184072E-5	3.0613441321227146E-6	6.122688264245429E-6	0.0	3.0613441321227146E-6
4	6.734957090669972E-5	3.0613441321227146E-6	6.122688264245429E-6	0.0	3.0613441321227146E-6
5	8.877897983155873E-5	3.0613441321227146E-6	6.122688264245429E-6	0.0	3.0613441321227146E-6
6	1.1939242115278587E-4	6.122688264245429E-6	1.8368064792736288E-5	0.0	3.0613441321227146E-6
7	1.28576453549154E-4	6.122688264245429E-6	2.1429408924859002E-5	0.0	3.0613441321227146E-6
8	1.5918989487038115E-4	9.184032396368144E-6	2.1429408924859002E-5	0.0	6.122688264245429E-6
9	1.6531258313462658E-4	1.5306720660613574E-5	2.755209718910443E-5	0.0	1.2245376528490858E-5
10-11	1.867419920594856E-4	3.367478545334986E-5	2.755209718910443E-5	0.0	2.1429408924859002E-5
12-13	2.1735543338071274E-4	7.653360330306787E-5	2.755209718910443E-5	6.122688264245429E-6	3.8266801651533935E-5
14-15	2.6939828362679887E-4	7.959494743519057E-5	2.755209718910443E-5	6.122688264245429E-6	5.204285024608615E-5
16-17	3.000117249480261E-4	8.724830776549736E-5	2.755209718910443E-5	6.122688264245429E-6	5.510419437820886E-5
18-19	3.5511591932623485E-4	9.949368429398823E-5	2.755209718910443E-5	6.122688264245429E-6	5.969621057639294E-5
20-21	4.270575064311187E-4	1.0255502842611094E-4	2.755209718910443E-5	6.122688264245429E-6	6.4288226774577E-5
22-23	5.234898465929841E-4	1.040857004921723E-4	2.755209718910443E-5	6.122688264245429E-6	6.4288226774577E-5
24-25	6.214528588209111E-4	1.1020838875641772E-4	2.755209718910443E-5	6.122688264245429E-6	6.888024297276108E-5
26-27	7.515599844361264E-4	1.3316846974733808E-4	2.755209718910443E-5	6.122688264245429E-6	7.653360330306787E-5
28-29	9.153418955046917E-4	1.3776048594552217E-4	3.6736129585472576E-5	6.122688264245429E-6	7.959494743519057E-5
30-31	0.001157188081942386	1.3929115801158352E-4	3.6736129585472576E-5	6.122688264245429E-6	7.959494743519057E-5
32-33	0.0014939359364758847	1.4388317420976757E-4	3.979747371759529E-5	6.122688264245429E-6	7.959494743519057E-5
34-35	0.0019271161311712488	1.515365345400744E-4	4.2858817849718005E-5	6.122688264245429E-6	8.112561950125193E-5
36-37	0.002543976973793976	1.5612855073825844E-4	4.2858817849718005E-5	9.184032396368144E-6	8.571763569943601E-5
38-39	0.0033215583833531453	1.5612855073825844E-4	4.2858817849718005E-5	9.184032396368144E-6	9.643234016186551E-5
40-41	0.004393028829596095	1.5918989487038115E-4	4.2858817849718005E-5	9.184032396368144E-6	1.0102435636004958E-4
42-43	0.005890026110204102	1.8521131999342424E-4	4.2858817849718005E-5	9.184032396368144E-6	1.0102435636004958E-4
44-45	0.007379370030481803	2.0970207305040595E-4	4.2858817849718005E-5	9.184032396368144E-6	1.0102435636004958E-4
46-47	0.009170256347773593	2.479688747019399E-4	4.2858817849718005E-5	9.184032396368144E-6	1.0255502842611094E-4
48-49	0.011594840900414781	2.479688747019399E-4	4.592016198184072E-5	9.184032396368144E-6	1.2092309321884721E-4
50-51	0.014770985437492098	2.479688747019399E-4	4.592016198184072E-5	9.184032396368144E-6	1.3163779768127673E-4
52-53	0.018766039529912237	2.57152907098308E-4	4.592016198184072E-5	9.184032396368144E-6	1.3163779768127673E-4
54-55	0.02350040823024002	2.6633693949467617E-4	4.592016198184072E-5	1.0714704462429501E-5	1.3776048594552217E-4
56-57	0.028513359246590965	2.9848105288196467E-4	5.510419437820886E-5	1.2245376528490858E-5	1.5000586247401304E-4
58-59	0.03464982355943094	3.061344132122715E-4	8.265629156731329E-5	1.2245376528490858E-5	1.5612855073825844E-4
60-61	0.04253737671584512	3.1991046180682365E-4	8.571763569943601E-5	1.2245376528490858E-5	1.5918989487038115E-4
62-63	0.05239490482128026	3.2450247800500776E-4	8.877897983155873E-5	1.2245376528490858E-5	1.5918989487038115E-4
64-65	0.06476426578712209	3.290944942031918E-4	8.877897983155873E-5	1.2245376528490858E-5	1.6837392726674931E-4
66-67	0.07972811590493792	3.3062516626925317E-4	8.877897983155873E-5	1.2245376528490858E-5	1.6837392726674931E-4
68-69	0.09792168408214322	3.3674785453349863E-4	8.877897983155873E-5	1.2245376528490858E-5	1.6837392726674931E-4
70-71	0.11987611352566126	3.3674785453349863E-4	8.877897983155873E-5	1.2245376528490858E-5	1.7143527139887202E-4
72-73	0.14800680475573688	3.3674785453349863E-4	9.184032396368144E-5	1.2245376528490858E-5	1.7755795966311743E-4
74-75	0.18348931391910522	3.474625589959281E-4	9.184032396368144E-5	1.5306720660613574E-5	1.8061930379524016E-4
76-77	0.22706448629573994	4.347108667614255E-4	9.184032396368144E-5	1.5306720660613574E-5	1.8061930379524016E-4
78-79	0.27977317889056275	4.4542557122385495E-4	9.184032396368144E-5	1.5306720660613574E-5	1.836806479273629E-4
80-81	0.34287054279774404	4.5001758742203906E-4	9.184032396368144E-5	1.5306720660613574E-5	1.8827266412554694E-4
82-83	0.4200852951702092	4.5001758742203906E-4	9.184032396368144E-5	1.5306720660613574E-5	2.0511005685222187E-4
84-85	0.517446753276174	4.66854980148714E-4	9.184032396368144E-5	1.8368064792736288E-5	2.112327451164673E-4
86-87	0.6393004951111865	4.928764052717571E-4	9.184032396368144E-5	2.1429408924859002E-5	2.112327451164673E-4
88-89	0.7868679969839638	5.14305814196616E-4	9.33709960297428E-5	2.1429408924859002E-5	2.1735543338071274E-4
90	0.9128162865956905	5.296125348572296E-4	9.796301222792687E-5	2.1429408924859002E-5	2.2041677751283544E-4
>>END_MODULE
