##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654139_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31477783
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14047660218002	32.0	32.0	32.0	32.0	32.0
2	31.29441330096214	32.0	32.0	32.0	32.0	32.0
3	35.08825751800881	37.0	32.0	37.0	32.0	37.0
4	35.779349867174574	37.0	37.0	37.0	32.0	37.0
5	35.93173880765364	37.0	37.0	37.0	37.0	37.0
6	39.32469681870543	41.0	41.0	41.0	37.0	41.0
7	39.40668277051151	41.0	41.0	41.0	37.0	41.0
8	39.41524166425571	41.0	41.0	41.0	37.0	41.0
9	39.51187235771973	41.0	41.0	41.0	37.0	41.0
10-11	39.54340297726813	41.0	41.0	41.0	37.0	41.0
12-13	39.52618920144408	41.0	41.0	41.0	37.0	41.0
14-15	39.45158609168885	41.0	41.0	41.0	37.0	41.0
16-17	39.37875828167441	41.0	41.0	41.0	37.0	41.0
18-19	39.1833242830348	41.0	41.0	41.0	37.0	41.0
20-21	39.13006751460228	41.0	41.0	41.0	37.0	41.0
22-23	39.08638202061435	41.0	41.0	41.0	37.0	41.0
24-25	39.0336733053913	41.0	41.0	41.0	37.0	41.0
26-27	38.97974960625403	41.0	41.0	41.0	37.0	41.0
28-29	39.06165456442723	41.0	41.0	41.0	37.0	41.0
30-31	39.1685977376488	41.0	41.0	41.0	37.0	41.0
32-33	39.20414819239335	41.0	41.0	41.0	37.0	41.0
34-35	39.19506365807274	41.0	41.0	41.0	37.0	41.0
36-37	39.18241476853691	41.0	41.0	41.0	37.0	41.0
38-39	39.152156173133285	41.0	41.0	41.0	37.0	41.0
40-41	39.149943580842404	41.0	41.0	41.0	37.0	41.0
42-43	39.113036153149665	41.0	41.0	41.0	37.0	41.0
44-45	39.096596049982296	41.0	41.0	41.0	37.0	41.0
46-47	39.03569997289834	41.0	41.0	41.0	37.0	41.0
48-49	39.00575644097934	41.0	41.0	41.0	37.0	41.0
50-51	38.9989684152788	41.0	41.0	41.0	37.0	41.0
52-53	39.00179113630715	41.0	41.0	41.0	37.0	41.0
54-55	38.99483812122347	41.0	41.0	41.0	37.0	41.0
56-57	38.94380128359103	41.0	41.0	41.0	34.5	41.0
58-59	38.901190690589615	41.0	41.0	41.0	32.0	41.0
60-61	38.8465814444429	41.0	41.0	41.0	32.0	41.0
62-63	38.778799590174444	41.0	41.0	41.0	32.0	41.0
64-65	38.78780564056878	41.0	41.0	41.0	32.0	41.0
66-67	38.767999941419006	41.0	41.0	41.0	32.0	41.0
68-69	38.68813343684337	41.0	41.0	41.0	32.0	41.0
70-71	38.60748438033263	41.0	41.0	41.0	32.0	41.0
72-73	38.610099145165336	41.0	41.0	41.0	32.0	41.0
74-75	38.503772946779634	41.0	41.0	41.0	32.0	41.0
76-77	37.691258291602054	41.0	39.0	41.0	29.5	41.0
78-79	38.23310019959157	41.0	39.0	41.0	32.0	41.0
80-81	38.68960042389262	41.0	41.0	41.0	32.0	41.0
82-83	38.74902446274568	41.0	41.0	41.0	32.0	41.0
84-85	38.70634523403379	41.0	41.0	41.0	32.0	41.0
86-87	38.656930127512474	41.0	41.0	41.0	32.0	41.0
88-89	38.57447954641532	41.0	41.0	41.0	32.0	41.0
90-91	38.44808422499132	41.0	41.0	41.0	32.0	41.0
92-93	38.349414426676745	41.0	37.0	41.0	32.0	41.0
94-95	38.21257991707993	41.0	37.0	41.0	32.0	41.0
96-97	38.23510855259406	41.0	37.0	41.0	32.0	41.0
98-99	38.15604596422817	41.0	37.0	41.0	32.0	41.0
100-101	37.098938924637736	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	44.0
13	306.0
14	839.0
15	2064.0
16	7140.0
17	17031.0
18	28345.0
19	39007.0
20	49137.0
21	61320.0
22	75641.0
23	93948.0
24	112649.0
25	134429.0
26	158761.0
27	186119.0
28	218870.0
29	255920.0
30	303261.0
31	368091.0
32	420246.0
33	498713.0
34	605723.0
35	758929.0
36	987534.0
37	1379140.0
38	2231934.0
39	5351792.0
40	1.713085E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	32.82485669624229	21.15665619439159	11.834006581277738	34.18448052808837
2	29.083448019988044	25.437317862988422	29.166445596080116	16.312788520943418
3	21.049906665312477	27.955822142484337	28.61491984501095	22.379351347192237
4	24.9385358049963	31.303538435942457	22.960209794566357	20.797715964494888
5	27.706300359467225	32.969328504856264	21.440514578957746	17.883856556718765
6	22.634159393015192	36.82149751312266	21.776430456021707	18.767912637840446
7	22.26569132902403	22.041949396499746	35.372284636437065	20.320074638039152
8	23.117441106298543	24.80377345744128	26.986633770288403	25.092151665971773
9	23.518810076300483	23.838235367465366	29.195258128566426	23.44769642766773
10-11	25.23616736286669	30.171233787335023	23.095752963288422	21.49684588650986
12-13	25.707606322095018	25.256575452599893	25.784141052645857	23.251677172659228
14-15	24.84715013125289	26.992853975770785	25.828861581516083	22.331134311460247
16-17	25.70396449012372	26.496363744955115	25.35406151199081	22.445610252930354
18-19	25.20155882642688	27.001182071812362	25.475494891110976	22.32176421064978
20-21	25.48565382924809	27.050012091111704	25.309054025221943	22.155280054418263
22-23	25.3925578365083	26.959121516249144	25.37989017142313	22.26843047581942
24-25	24.885125712250446	27.017923865944866	25.756637312997256	22.340313108807436
26-27	25.162128706462205	27.109932042666184	25.55924463639413	22.16869461447748
28-29	25.421640154125363	26.73004327250585	25.531660034044005	22.316656539324782
30-31	24.928209935919913	26.87452945884469	25.867277329286832	22.329983275948564
32-33	25.093869045854756	27.125432477776517	25.576267195982265	22.204431280386462
34-35	25.275312432263735	26.843497841001064	25.53588033820552	22.34530938852968
36-37	24.949336489167614	26.79850737899807	25.877314803269343	22.374841328564976
38-39	25.12275912145241	27.028582449596218	25.54751174950436	22.30114667944701
40-41	25.38930870703315	26.677048698124644	25.5677091363137	22.365933458528513
42-43	25.077290286135046	26.75456184793841	25.860362232266915	22.307785633659627
44-45	25.108834805789048	26.888571886726503	25.74171238828513	22.260880919199316
46-47	25.355866072270715	26.674322966137737	25.612896562632763	22.356914398958782
48-49	24.970705886785638	26.713796164437664	25.933983757318046	22.38151419145865
50-51	25.178238967864058	26.861433681125675	25.709491481571987	22.25083586943828
52-53	25.369181834765055	26.66491843813396	25.6234787187248	22.342421008376185
54-55	24.96072519223776	26.784686626093634	26.00315043508618	22.251437746582432
56-57	24.95444537623845	26.95506964698203	25.86380155974279	22.226683417036732
58-59	25.299620579426275	26.60474856685797	25.73746523420899	22.35816561950676
60-61	24.995238705343382	26.766060684769315	25.89402500169723	22.344675608190066
62-63	25.078598540280485	26.898349795611786	25.770555119291917	22.252496544815813
64-65	25.272108602572718	26.73364745755109	25.72648942439748	22.26775451547871
66-67	24.95885879703758	26.83597145340592	26.03325416431574	22.171915585240757
68-69	25.17724766855628	27.011599892317438	25.763621402398257	22.047531036728028
70-71	25.467860453357517	26.796158507075567	25.51424081546839	22.221740224098525
72-73	24.981688243483006	26.850519090029003	25.99355604709533	22.17423661939266
74-75	25.062602057695027	27.001128215002545	25.81528969513701	22.120980032165413
76-77	25.424898245768308	26.71662449366708	25.668451746139453	22.190025514425162
78-79	25.003377787762975	26.785261300673685	26.064117512005243	22.147243399558096
80-81	25.168796900834877	26.925120828926246	25.812599934273166	22.093482335965707
82-83	25.440099176151517	26.655091858460107	25.740987638706564	22.163821326681813
84-85	25.120042920686238	26.843854702942604	26.011230749820136	22.02487162655102
86-87	25.20686574400745	27.020711719119483	25.846048306515108	21.926374230357965
88-89	25.41969521805268	26.865400590632447	25.663479858159004	22.051424333155865
90-91	25.098935652488613	26.939273328112083	25.975839848695827	21.985951170703476
92-93	25.311474445325455	27.058562224664932	25.714323654877475	21.91563967513214
94-95	25.657626491943976	26.814986279759918	25.581170937943483	21.94621629035263
96-97	25.257656226750854	26.88729865143103	25.93145701424197	21.92358810757614
98-99	25.429240072545138	27.122839440302354	25.68492995106612	21.76299053608639
100-101	25.788329530209957	26.871784463102628	25.571016514709044	21.768869491978368
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	47.0
1	43.0
2	33.0
3	28.5
4	36.0
5	53.5
6	61.0
7	66.0
8	95.5
9	126.0
10	152.0
11	173.5
12	207.5
13	261.0
14	332.0
15	420.0
16	522.0
17	677.0
18	852.5
19	1042.0
20	1354.0
21	1745.5
22	2286.5
23	3075.5
24	4060.5
25	5631.0
26	7580.0
27	10131.0
28	14172.5
29	19339.0
30	26241.0
31	36594.5
32	52009.5
33	74162.5
34	109831.0
35	167969.5
36	254004.0
37	372545.5
38	537534.0
39	752122.0
40	1002590.5
41	1264680.0
42	1527884.5
43	1762050.0
44	1941905.0
45	2056951.0
46	2100598.5
47	2093005.5
48	2017188.5
49	1872996.5
50	1687220.5
51	1507488.5
52	1341509.5
53	1162920.0
54	994432.0
55	842941.0
56	687440.5
57	552153.0
58	452508.0
59	379114.0
60	320605.0
61	268051.5
62	220721.5
63	179428.5
64	153709.5
65	131209.0
66	109397.5
67	90898.5
68	71126.5
69	53878.5
70	46860.0
71	42160.5
72	32568.5
73	22259.5
74	12890.5
75	6798.0
76	3634.5
77	1421.5
78	729.5
79	480.5
80	333.0
81	287.0
82	248.0
83	192.0
84	152.0
85	141.0
86	127.5
87	107.5
88	95.5
89	77.0
90	72.5
91	85.0
92	86.0
93	70.5
94	59.0
95	67.5
96	68.0
97	68.0
98	90.0
99	141.5
100	651.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017393219846518416
2	0.006877866843417785
3	6.480761367469876E-4
4	0.0016328977170978018
5	2.763854112597447E-4
6	0.001130956395499645
7	0.0
8	0.0243092088156272
9	0.0
10-11	0.0
12-13	0.003886868398578134
14-15	0.0
16-17	0.0010896574260010625
18-19	0.0
20-21	3.653370378720763E-4
22-23	3.176843807583272E-6
24-25	1.1118953326541453E-5
26-27	0.0054324029109673955
28-29	9.21284704199149E-4
30-31	2.017295817815378E-4
32-33	4.892339463678239E-4
34-35	0.0
36-37	0.0
38-39	9.054004851612327E-4
40-41	0.0
42-43	7.624425138199853E-5
44-45	0.0019235789254916714
46-47	0.0
48-49	0.0014899397457565546
50-51	0.0012040238030740602
52-53	6.512529805545708E-4
54-55	7.179667005138196E-4
56-57	0.008442462418652546
58-59	5.734203072687807E-4
60-61	0.0
62-63	0.004862159447506198
64-65	6.353687615166544E-6
66-67	0.0026145424536410332
68-69	2.4461697318391195E-4
70-71	6.671371995924871E-5
72-73	1.7313798751328834E-4
74-75	5.829508386915306E-4
76-77	3.160959588545356E-4
78-79	2.5414750460666177E-4
80-81	0.0016884924837305092
82-83	0.005297387049145107
84-85	5.956582139218636E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	5.5594766632707264E-5
96-97	0.004738262539010451
98-99	6.353687615166544E-6
100-101	4.6699603971474106E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.1477783E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.64189630826653
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.06304749879894	22.192654486449463
2	14.729674173834164	10.205276907690292
3	6.726819893331269	6.990893916874991
4	3.7762880242676182	5.2327111266731015
5	2.3125014597374314	4.005471789046954
6	1.6288858106993196	3.3856616011351495
7	1.121735608108609	2.7201334034972113
8	0.8516301104294126	2.360166558279463
9	0.6353519305122671	1.9808816126456625
>10	3.7646722585885577	23.6805538682521
>50	0.24924386272924798	5.935723922168517
>100	0.13010665268599986	8.168282521888107
>500	0.007759270167500933	1.8050384605706842
>1k	0.002225997052635727	1.1201315856256022
>5k	3.8299371324902034E-5	0.0683813572946206
>10k+	1.9149685662451017E-5	0.14803688190804287
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2237906653082906E-5	0.0	2.541475046066618E-5	3.1768438075832724E-6	0.0
2	3.4945281883415994E-5	3.1768438075832724E-6	5.400634472891563E-5	3.1768438075832724E-6	0.0
3	6.036003234408218E-5	3.1768438075832724E-6	6.036003234408218E-5	3.1768438075832724E-6	0.0
4	9.848215803508144E-5	1.270737523033309E-5	8.577478280474835E-5	6.353687615166545E-6	0.0
5	1.0801268945783126E-4	1.270737523033309E-5	8.895162661233163E-5	9.530531422749817E-6	3.1768438075832724E-6
6	1.493116589564138E-4	1.9061062845499634E-5	8.895162661233163E-5	9.530531422749817E-6	3.1768438075832724E-6
7	1.5248850276399707E-4	1.9061062845499634E-5	1.0483584565024798E-4	1.588421903791636E-5	3.1768438075832724E-6
8	1.8108009703224652E-4	2.2237906653082906E-5	1.2389690849574762E-4	1.9061062845499634E-5	3.1768438075832724E-6
9	1.937874722625796E-4	2.2237906653082906E-5	1.366042837260807E-4	1.9061062845499634E-5	3.1768438075832724E-6
10-11	2.3032117604978724E-4	2.7003172364457816E-5	1.540769246677887E-4	1.9061062845499634E-5	9.530531422749817E-6
12-13	2.6526645793320324E-4	2.8591594268249452E-5	1.7790325322466322E-4	1.9061062845499634E-5	9.530531422749817E-6
14-15	3.399222874114101E-4	3.176843807583272E-5	2.1602537891566252E-4	1.9061062845499634E-5	1.270737523033309E-5
16-17	4.3681602354269993E-4	3.4945281883415994E-5	3.208612245659105E-4	1.9061062845499634E-5	1.270737523033309E-5
18-19	5.114718530209068E-4	3.4945281883415994E-5	3.7169072548724286E-4	2.2237906653082906E-5	1.270737523033309E-5
20-21	6.131308548635716E-4	3.4945281883415994E-5	4.415812892540748E-4	2.541475046066618E-5	1.270737523033309E-5
22-23	7.751498890503184E-4	3.812212569099927E-5	5.448287130005313E-4	2.541475046066618E-5	1.270737523033309E-5
24-25	0.001021355284138022	3.971054759479091E-5	5.829508386915304E-4	2.8591594268249452E-5	1.270737523033309E-5
26-27	0.001361277571549432	4.129896949858254E-5	6.115424329597799E-4	3.018001617204109E-5	1.270737523033309E-5
28-29	0.001775855688439049	4.129896949858254E-5	6.655487776886955E-4	3.335685997962436E-5	1.9061062845499634E-5
30-31	0.0023540412614192047	4.447581330616581E-5	6.861982624379868E-4	3.4945281883415994E-5	1.9061062845499634E-5
32-33	0.0030180016172041088	4.7652657113749086E-5	7.004940595721116E-4	4.129896949858254E-5	1.9061062845499634E-5
34-35	0.003842392585271968	5.082950092133236E-5	8.132720147413177E-4	4.129896949858254E-5	1.9061062845499634E-5
36-37	0.004995586887424696	5.082950092133236E-5	8.545709842399002E-4	4.129896949858254E-5	1.9061062845499634E-5
38-39	0.00646646557033575	5.400634472891563E-5	8.81574156604358E-4	4.129896949858254E-5	1.9061062845499634E-5
40-41	0.00839798660534638	5.400634472891563E-5	9.403457670446486E-4	4.447581330616581E-5	1.9061062845499634E-5
42-43	0.010744085757246627	5.8771610440290544E-5	0.0010324742374645634	4.447581330616581E-5	2.064948474929127E-5
44-45	0.01363024835643603	6.353687615166544E-5	0.0010705963631555627	4.447581330616581E-5	2.2237906653082906E-5
46-47	0.016849979555421675	6.830214186304036E-5	0.0011595479897678943	4.447581330616581E-5	2.2237906653082906E-5
48-49	0.020741613219711186	7.306740757441527E-5	0.0012040238030740602	4.447581330616581E-5	2.541475046066618E-5
50-51	0.02567842849669559	7.306740757441527E-5	0.0012246732878233514	4.7652657113749086E-5	2.541475046066618E-5
52-53	0.03186374339006022	7.306740757441527E-5	0.0012802680544560588	4.924107901754072E-5	2.7003172364457816E-5
54-55	0.03909582831802354	7.306740757441527E-5	0.0013168017582432664	5.082950092133236E-5	3.176843807583272E-5
56-57	0.04686321142756464	7.306740757441527E-5	0.0013644544153570155	5.400634472891563E-5	3.176843807583272E-5
58-59	0.05604746687528788	7.306740757441527E-5	0.0014089302286631813	5.400634472891563E-5	3.176843807583272E-5
60-61	0.0678955058556697	7.624425138199853E-5	0.00148517448004518	5.400634472891563E-5	3.335685997962436E-5
62-63	0.08248992630770725	7.942109518958181E-5	0.0016201903418674689	5.7183188536498904E-5	3.4945281883415994E-5
64-65	0.09965600182198345	7.942109518958181E-5	0.0016837272180191343	6.036003234408218E-5	3.812212569099927E-5
66-67	0.11965264516881635	8.577478280474835E-5	0.0016996114370570507	6.036003234408218E-5	3.812212569099927E-5
68-69	0.14284519338607804	1.0007057993887308E-4	0.0017186724999025502	6.036003234408218E-5	3.971054759479091E-5
70-71	0.17147808662382608	1.0324742374645635E-4	0.0017250261875177168	6.036003234408218E-5	4.129896949858254E-5
72-73	0.2074796690732635	1.0483584565024798E-4	0.0017647367351125078	6.036003234408218E-5	4.7652657113749086E-5
74-75	0.25098654501811646	1.0483584565024798E-4	0.0017663251570162995	6.353687615166544E-5	4.7652657113749086E-5
76-77	0.3042923956874599	1.0801268945783126E-4	0.0017790325322466325	6.671371995924872E-5	4.7652657113749086E-5
78-79	0.36846622902254583	1.096011113616229E-4	0.0017933283293807573	6.671371995924872E-5	4.7652657113749086E-5
80-81	0.4439067389212258	1.1436637707299781E-4	0.0018108009703224652	6.671371995924872E-5	5.082950092133236E-5
82-83	0.5365403275065465	1.1754322088058109E-4	0.0018330388769755481	6.671371995924872E-5	5.082950092133236E-5
84-85	0.6507065634196665	1.2230848659195597E-4	0.0018457462522058813	6.671371995924872E-5	5.082950092133236E-5
86-87	0.7916408852554833	1.2389690849574762E-4	0.0018632188931475891	6.671371995924872E-5	5.082950092133236E-5
88-89	0.9599135364774577	1.286621742071225E-4	0.0018743378464741306	6.671371995924872E-5	5.082950092133236E-5
90	1.104258200140715	1.3342743991849744E-4	0.0018806915340892972	6.671371995924872E-5	5.082950092133236E-5
>>END_MODULE
