##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654110_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39490969
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66456242185397	32.0	32.0	32.0	32.0	32.0
2	31.69831431586295	32.0	32.0	32.0	32.0	32.0
3	35.81821296914745	37.0	37.0	37.0	32.0	37.0
4	36.38474287627635	37.0	37.0	37.0	37.0	37.0
5	36.56128873414071	37.0	37.0	37.0	37.0	37.0
6	40.18000355980123	41.0	41.0	41.0	37.0	41.0
7	40.21350519406095	41.0	41.0	41.0	37.0	41.0
8	40.24872030868627	41.0	41.0	41.0	41.0	41.0
9	40.294527212031696	41.0	41.0	41.0	41.0	41.0
10-11	40.323074941513845	41.0	41.0	41.0	41.0	41.0
12-13	40.30126438528262	41.0	41.0	41.0	41.0	41.0
14-15	40.296672753205925	41.0	41.0	41.0	41.0	41.0
16-17	40.29496731265318	41.0	41.0	41.0	41.0	41.0
18-19	40.29480703550222	41.0	41.0	41.0	41.0	41.0
20-21	40.26502964513229	41.0	41.0	41.0	41.0	41.0
22-23	40.271034790258	41.0	41.0	41.0	41.0	41.0
24-25	40.25742332379841	41.0	41.0	41.0	41.0	41.0
26-27	40.1788710223849	41.0	41.0	41.0	41.0	41.0
28-29	40.18899526116971	41.0	41.0	41.0	41.0	41.0
30-31	40.10262389357932	41.0	41.0	41.0	39.0	41.0
32-33	40.12889202592117	41.0	41.0	41.0	41.0	41.0
34-35	40.14085522945765	41.0	41.0	41.0	41.0	41.0
36-37	40.124088294718725	41.0	41.0	41.0	41.0	41.0
38-39	40.04837113518283	41.0	41.0	41.0	37.0	41.0
40-41	40.07429888590478	41.0	41.0	41.0	37.0	41.0
42-43	40.0529632610433	41.0	41.0	41.0	37.0	41.0
44-45	40.05542726743423	41.0	41.0	41.0	37.0	41.0
46-47	40.04389482061076	41.0	41.0	41.0	37.0	41.0
48-49	39.95675392265001	41.0	41.0	41.0	37.0	41.0
50-51	39.91520665142453	41.0	41.0	41.0	37.0	41.0
52-53	39.96085935495784	41.0	41.0	41.0	37.0	41.0
54-55	39.92823761807415	41.0	41.0	41.0	37.0	41.0
56-57	39.916154323789826	41.0	41.0	41.0	37.0	41.0
58-59	39.917016698172176	41.0	41.0	41.0	37.0	41.0
60-61	39.89465692776493	41.0	41.0	41.0	37.0	41.0
62-63	39.853570470757504	41.0	41.0	41.0	37.0	41.0
64-65	39.79952464574875	41.0	41.0	41.0	37.0	41.0
66-67	39.77450988857731	41.0	41.0	41.0	37.0	41.0
68-69	39.750286616162796	41.0	41.0	41.0	37.0	41.0
70-71	39.72531372932379	41.0	41.0	41.0	37.0	41.0
72-73	39.69381459087519	41.0	41.0	41.0	37.0	41.0
74-75	39.673358774761894	41.0	41.0	41.0	37.0	41.0
76-77	39.0940286879261	41.0	39.0	41.0	34.5	41.0
78-79	39.441704076696624	41.0	41.0	41.0	37.0	41.0
80-81	39.64219125643638	41.0	41.0	41.0	37.0	41.0
82-83	39.71739727379189	41.0	41.0	41.0	37.0	41.0
84-85	39.754387363855265	41.0	41.0	41.0	37.0	41.0
86-87	39.76088324143173	41.0	41.0	41.0	37.0	41.0
88-89	39.640797570705345	41.0	41.0	41.0	37.0	41.0
90-91	39.65646744449345	41.0	41.0	41.0	37.0	41.0
92-93	39.6080885100591	41.0	41.0	41.0	37.0	41.0
94-95	39.59680456308884	41.0	41.0	41.0	37.0	41.0
96-97	39.557947501870615	41.0	41.0	41.0	37.0	41.0
98-99	39.50953069801858	41.0	41.0	41.0	37.0	41.0
100-101	38.97208999860196	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	5.0
16	1.0
17	24.0
18	75.0
19	216.0
20	572.0
21	1743.0
22	4188.0
23	9161.0
24	17805.0
25	32192.0
26	53981.0
27	83891.0
28	122258.0
29	168380.0
30	219631.0
31	277660.0
32	341415.0
33	418307.0
34	497590.0
35	608074.0
36	767856.0
37	1041589.0
38	1627190.0
39	3914040.0
40	2.9283123E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.44035711417169	11.196257983469431	8.177274511249639	41.18611039110924
2	23.592044550742727	13.458641645384798	34.23699226017979	28.71232154369269
3	20.375252079926064	17.796739591988008	24.290121480247105	37.53788684783883
4	23.344524668412163	25.56822548466714	22.924362782792187	28.162887064128512
5	24.189125366865525	30.36775319440756	24.182615017625928	21.260506421100985
6	20.57444829981255	32.72716098711075	24.689791734408946	22.008598978667756
7	16.655025608513178	23.634737856141236	40.52090238656843	19.189334148777153
8	19.661041996715756	24.001999039324662	29.980798901136108	26.356160062823474
9	19.286308218975332	22.566561990413554	32.930030154489245	25.21709963612187
10-11	22.60217404824299	30.135502357615273	23.674183261944467	23.58814033219727
12-13	22.460654232110638	24.11955376430495	27.0250940157989	26.39469798778551
14-15	21.45193119976367	25.76719122794885	27.467367285923018	25.313510286364462
16-17	22.22705461853281	26.090416194542605	26.74696665946619	24.935562527458405
18-19	22.04189175863938	26.484489019718687	26.43198859138407	25.04163063025786
20-21	22.146231711863035	26.377568755023457	26.569260683372953	24.906938849740555
22-23	22.17243010674162	26.56789834658147	26.498100109926398	24.76157143675051
24-25	22.15910082218645	26.270358445172754	26.277501859224806	25.293038873415995
26-27	21.89216096470056	26.501521145252223	26.477546043501743	25.128771846545472
28-29	22.00876620620355	26.64854517276562	26.39233270637387	24.950355914656956
30-31	22.167621421007	26.430872339340965	26.29105053060801	25.11045570904402
32-33	22.189430196053177	26.320318956974692	26.458784792036884	25.03146605493524
34-35	22.30969313515705	26.486715228486794	26.217391626931207	24.986200009424937
36-37	22.126575319030536	26.35710205034473	26.16287283302671	25.353449797598028
38-39	22.132384225277544	26.458931590988506	26.317440797474823	25.091243386259126
40-41	22.302800420015018	26.76790103580391	26.214812556258117	24.71448598792296
42-43	22.29260948243635	26.328216965250967	26.276078462394782	25.103095089917897
44-45	22.28376442218979	26.309651961186365	26.4173360749897	24.98924754163414
46-47	22.31652166787427	26.36974397050765	26.321322972626632	24.992411388991453
48-49	22.16285829805797	26.307447659742156	26.210087425304756	25.31960661689512
50-51	22.182582718473164	26.273308939608597	26.443825291737433	25.100283050180806
52-53	22.53993315881408	26.449732692611487	26.104141824628574	24.90619232394586
54-55	22.339379669311228	26.402287064670404	26.11169530937567	25.146637956642692
56-57	22.287777202550714	26.208133307789755	26.45350002850672	25.050589461152807
58-59	22.400241473214507	26.276698144997752	26.3460469934578	24.977013388329937
60-61	22.414453606855204	26.128461753595957	26.286178100506742	25.170906539042104
62-63	22.300326220875743	26.119259257655465	26.43556487850344	25.144849642965355
64-65	22.52944502984994	26.33062773352657	26.27198659636939	24.8679406402541
66-67	22.389026207796075	26.18443608467651	26.24641033214072	25.180127375386697
68-69	22.42051640303958	26.1819816306174	26.470427140524627	24.927074825818384
70-71	22.656787172670047	26.462651800925173	26.195235126600576	24.6853258998042
72-73	22.572211383316525	26.260334356444886	26.088167145252882	25.07928711498571
74-75	22.44934151906984	26.24928499475412	26.29028297583683	25.011090510339212
76-77	22.47664225820015	26.425965591482964	26.211110666701558	24.88628148361532
78-79	22.470873593171987	26.23603199597437	26.159066295867955	25.134028114985686
80-81	22.531585663813782	26.270799572112267	26.29013724914055	24.907477514933397
82-83	22.694899123348787	26.51158610122646	26.053689967912796	24.739824807511962
84-85	22.713222100107068	26.402142485376018	25.958638430599734	24.92599698391718
86-87	22.695915579726563	26.289736346240606	26.178712348772248	24.83563572526058
88-89	22.88928514263603	26.34456880508554	26.08566783965215	24.680478212626284
90-91	22.78987724594815	26.2205435191833	26.003450101503176	24.98612913336537
92-93	22.77057391138056	26.267640878487246	25.948602300065826	25.013182910066366
94-95	22.941793350272057	26.436274582170928	25.842851564366526	24.77908050319049
96-97	22.945253557165053	26.475402231068262	25.59668240178524	24.982661809981437
98-99	22.9491228544862	26.5714031078073	25.799750656559617	24.679723381146886
100-101	23.08928529963395	26.714038088049954	25.595543122783337	24.60113348953276
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	100.0
1	68.0
2	34.5
3	38.5
4	48.0
5	58.5
6	70.0
7	81.5
8	100.0
9	130.5
10	164.5
11	194.5
12	221.0
13	246.0
14	286.5
15	344.0
16	407.5
17	499.0
18	636.5
19	831.5
20	1150.0
21	1593.5
22	2294.5
23	3378.5
24	5173.0
25	7657.0
26	11278.0
27	16572.5
28	23461.0
29	33770.0
30	48901.0
31	68066.0
32	95991.5
33	133957.5
34	181644.0
35	249971.0
36	345779.5
37	476653.0
38	651614.5
39	885431.0
40	1157562.5
41	1440364.5
42	1738662.0
43	2026068.0
44	2264082.0
45	2417452.0
46	2507631.0
47	2546258.0
48	2476692.0
49	2337158.5
50	2158832.0
51	1929053.5
52	1735061.5
53	1566830.5
54	1368757.5
55	1168653.5
56	987142.0
57	813177.5
58	649236.5
59	515602.0
60	418900.0
61	360763.0
62	316136.0
63	271677.0
64	232908.5
65	202829.0
66	174252.0
67	140028.0
68	99326.0
69	67092.0
70	47743.5
71	34294.0
72	26047.5
73	20265.5
74	13455.5
75	6939.0
76	3528.0
77	1350.0
78	180.0
79	57.5
80	22.5
81	13.0
82	6.0
83	6.0
84	4.5
85	3.0
86	3.5
87	2.0
88	1.0
89	0.0
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	1.0
97	1.0
98	0.0
99	0.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05945409949297521
2	0.0
3	5.570893942865773E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.494927207281239E-4
12-13	0.0
14-15	0.0
16-17	0.0014041184960541231
18-19	0.00206376298337982
20-21	0.0
22-23	0.0
24-25	1.2154677693525323E-4
26-27	0.0
28-29	2.823430339225153E-4
30-31	0.001787750510756016
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0028373575740823177
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.002203035331951465
48-49	0.0
50-51	0.0015737775388595808
52-53	5.001143425981773E-4
54-55	0.0
56-57	7.178856512738394E-4
58-59	1.1774844015602655E-4
60-61	0.00114583159506671
62-63	0.0033260769063428145
64-65	0.001957409553561474
66-67	9.432536335079548E-4
68-69	3.747692288836975E-4
70-71	8.862785818195548E-4
72-73	0.0
74-75	0.0
76-77	9.33124735430017E-4
78-79	2.532224519484442E-6
80-81	0.0017814199494573051
82-83	0.0011724199525212967
84-85	2.7854469714328866E-5
86-87	0.00103188149168991
88-89	0.0
90-91	0.0020928835653538915
92-93	0.0011319043602095456
94-95	0.0
96-97	2.8107692166277307E-4
98-99	0.0011584927176641324
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.9490969E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.001895353359945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.29920436358931	17.777931101970427
2	15.494353223629439	8.987312215193928
3	6.941062717481267	6.039119237204997
4	3.8736442386804812	4.4937209938542795
5	2.444593616120403	3.544892411810786
6	1.7240709373892698	3.0000794944759672
7	1.2522643624352923	2.542262799586325
8	0.9352841728708002	2.1700010965802212
9	0.7929217423115386	2.0696610054530775
>10	4.682235684525723	25.166888831627098
>50	0.35177536138407	7.021531040328948
>100	0.18606883955385917	9.956391416092705
>500	0.014388580463887494	2.866565718657936
>1k	0.007994250410479851	4.0994192883030705
>5k	1.287152102661254E-4	0.23419415048229422
>10k+	9.193943590437529E-6	0.03002919837792576
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.090228654556438E-5	0.0	0.0	0.0	2.532224519484442E-6
2	1.2407900145473767E-4	0.0	7.596673558453327E-6	0.0	1.266112259742221E-5
3	1.6965904280545762E-4	2.532224519484442E-6	7.596673558453327E-6	2.532224519484442E-6	1.5193347116906653E-5
4	2.557546764679287E-4	2.532224519484442E-6	1.0128898077937768E-5	2.532224519484442E-6	2.279002067535998E-5
5	2.886735952212264E-4	2.532224519484442E-6	1.0128898077937768E-5	2.532224519484442E-6	2.279002067535998E-5
6	3.5197920820833744E-4	2.532224519484442E-6	2.532224519484442E-5	2.532224519484442E-6	2.279002067535998E-5
7	3.9249480052008854E-4	7.596673558453327E-6	2.532224519484442E-5	2.532224519484442E-6	2.279002067535998E-5
8	4.532681889877151E-4	1.266112259742221E-5	2.532224519484442E-5	2.532224519484442E-6	2.279002067535998E-5
9	4.8365488322152844E-4	1.5193347116906653E-5	2.532224519484442E-5	2.532224519484442E-6	3.291891875329775E-5
10-11	5.494927207281239E-4	1.6459459376648875E-5	2.532224519484442E-5	2.532224519484442E-6	3.418503101303997E-5
12-13	6.254594563126572E-4	3.545114327278219E-5	2.532224519484442E-5	7.596673558453327E-6	3.545114327278219E-5
14-15	7.520706822868793E-4	3.798336779226663E-5	2.532224519484442E-5	7.596673558453327E-6	3.798336779226663E-5
16-17	8.381663159493503E-4	3.924948005200885E-5	2.532224519484442E-5	7.596673558453327E-6	3.924948005200885E-5
18-19	9.229958373520792E-4	4.051559231175107E-5	2.532224519484442E-5	7.596673558453327E-6	4.051559231175107E-5
20-21	0.0010230187058717145	4.051559231175107E-5	2.532224519484442E-5	7.596673558453327E-6	4.051559231175107E-5
22-23	0.0011470977073264523	4.1781704571493297E-5	2.532224519484442E-5	7.596673558453327E-6	4.051559231175107E-5
24-25	0.001318022862391652	4.9378378129946626E-5	2.7854469714328862E-5	7.596673558453327E-6	4.051559231175107E-5
26-27	0.0014724885580802032	6.837006202607993E-5	3.291891875329775E-5	7.596673558453327E-6	4.1781704571493297E-5
28-29	0.0017003887648338028	6.837006202607993E-5	4.431392909097774E-5	7.596673558453327E-6	4.3047816831235514E-5
30-31	0.002061230758860336	6.837006202607993E-5	4.81122658702044E-5	7.596673558453327E-6	4.3047816831235514E-5
32-33	0.002563877325977998	7.849896010401771E-5	4.81122658702044E-5	7.596673558453327E-6	4.3047816831235514E-5
34-35	0.0032273201500829215	8.356340914298659E-5	4.81122658702044E-5	7.596673558453327E-6	4.431392909097774E-5
36-37	0.004237677733357214	8.482952140272881E-5	4.81122658702044E-5	7.596673558453327E-6	4.9378378129946626E-5
38-39	0.005719029077255612	8.736174592221326E-5	4.81122658702044E-5	7.596673558453327E-6	5.444282716891551E-5
40-41	0.007894209939492748	8.862785818195547E-5	4.81122658702044E-5	7.596673558453327E-6	5.824116394814217E-5
42-43	0.010940476036432532	1.0635342981834658E-4	5.064449038968884E-5	7.596673558453327E-6	5.950727620788439E-5
44-45	0.014528638180541988	1.2914345049370654E-4	5.064449038968884E-5	7.596673558453327E-6	7.343451106504882E-5
46-47	0.018400409470833698	1.506673589093243E-4	5.064449038968884E-5	7.596673558453327E-6	8.103118462350215E-5
48-49	0.02365224312424443	1.5193347116906653E-4	5.191060264943106E-5	7.596673558453327E-6	8.482952140272881E-5
50-51	0.030875413566073803	1.5319958342880876E-4	5.3176714909173284E-5	7.596673558453327E-6	9.116008270143992E-5
52-53	0.040220588155231135	1.5953014472751986E-4	5.5708939428657724E-5	7.596673558453327E-6	9.116008270143992E-5
54-55	0.0511800558755598	1.607962569872621E-4	5.950727620788439E-5	7.596673558453327E-6	1.0255509303911992E-4
56-57	0.06314608284238353	1.7598960410416875E-4	6.58378375065955E-5	1.0128898077937768E-5	1.2028066467551101E-4
58-59	0.07706698713824925	1.7852182862365315E-4	7.596673558453326E-5	1.0128898077937768E-5	1.253451137144799E-4
60-61	0.09515466688092662	1.8105405314313761E-4	7.596673558453326E-5	1.0128898077937768E-5	1.266112259742221E-4
62-63	0.11901328630350903	1.8232016540287984E-4	7.596673558453326E-5	1.0128898077937768E-5	1.266112259742221E-4
64-65	0.14845925912833385	1.8232016540287984E-4	7.72328478442755E-5	1.0128898077937768E-5	1.266112259742221E-4
66-67	0.18315073504527074	1.873846144418487E-4	7.849896010401771E-5	1.0128898077937768E-5	1.278773382339643E-4
68-69	0.22351565999811246	1.873846144418487E-4	7.976507236375993E-5	1.0128898077937768E-5	1.2914345049370654E-4
70-71	0.2725825745121625	1.873846144418487E-4	8.103118462350215E-5	1.0128898077937768E-5	1.31675675013191E-4
72-73	0.33480439540493423	1.873846144418487E-4	8.103118462350215E-5	1.0128898077937768E-5	1.3294178727293323E-4
74-75	0.4142921891838106	1.9498128800030204E-4	8.103118462350215E-5	1.266112259742221E-5	1.3674012405215987E-4
76-77	0.512689369561937	2.279002067535998E-4	8.103118462350215E-5	1.266112259742221E-5	1.392723485716443E-4
78-79	0.6319672733277322	2.3043243127308423E-4	8.103118462350215E-5	1.266112259742221E-5	1.443367976106132E-4
80-81	0.7737591852962635	2.3043243127308423E-4	8.103118462350215E-5	1.266112259742221E-5	1.4686902213009763E-4
82-83	0.9451831885918018	2.3043243127308423E-4	8.103118462350215E-5	1.266112259742221E-5	1.5699792020803542E-4
84-85	1.1595739775339522	2.3802910483153756E-4	8.103118462350215E-5	1.266112259742221E-5	1.620623692470043E-4
86-87	1.427061716312912	2.456257783899909E-4	8.103118462350215E-5	1.266112259742221E-5	1.620623692470043E-4
88-89	1.745878912214081	2.557546764679286E-4	8.229729688324436E-5	1.266112259742221E-5	1.7219126732494205E-4
90	2.0191401228974657	2.63351350026382E-4	8.862785818195547E-5	1.266112259742221E-5	1.7978794088339538E-4
>>END_MODULE
