##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654124_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34485844
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.346576467723974	32.0	32.0	32.0	32.0	32.0
2	31.330910677436226	32.0	32.0	32.0	32.0	32.0
3	35.12170901196445	37.0	32.0	37.0	32.0	37.0
4	35.8654083687208	37.0	37.0	37.0	32.0	37.0
5	36.09568186876911	37.0	37.0	37.0	37.0	37.0
6	39.17349869703058	41.0	41.0	41.0	37.0	41.0
7	39.31153878675551	41.0	41.0	41.0	37.0	41.0
8	39.502667297340906	41.0	41.0	41.0	37.0	41.0
9	39.703259633141066	41.0	41.0	41.0	37.0	41.0
10-11	39.70831943391033	41.0	41.0	41.0	37.0	41.0
12-13	39.61962617182865	41.0	41.0	41.0	37.0	41.0
14-15	39.520392686343996	41.0	41.0	41.0	37.0	41.0
16-17	39.58857037107748	41.0	41.0	41.0	37.0	41.0
18-19	39.57275515715956	41.0	41.0	41.0	37.0	41.0
20-21	39.51279732344669	41.0	41.0	41.0	37.0	41.0
22-23	39.39323904904285	41.0	41.0	41.0	37.0	41.0
24-25	39.41876066597065	41.0	41.0	41.0	37.0	41.0
26-27	39.31547182084336	41.0	41.0	41.0	37.0	41.0
28-29	39.424778106054184	41.0	41.0	41.0	37.0	41.0
30-31	39.40507544196976	41.0	41.0	41.0	37.0	41.0
32-33	39.4130518597718	41.0	41.0	41.0	37.0	41.0
34-35	39.271566341829995	41.0	41.0	41.0	37.0	41.0
36-37	39.20518607577068	41.0	41.0	41.0	37.0	41.0
38-39	39.1701685769964	41.0	41.0	41.0	37.0	41.0
40-41	39.11192440295212	41.0	41.0	41.0	37.0	41.0
42-43	39.0911234766358	41.0	41.0	41.0	37.0	41.0
44-45	39.09700971795847	41.0	41.0	41.0	37.0	41.0
46-47	39.053446031362895	41.0	41.0	41.0	37.0	41.0
48-49	39.00576410425101	41.0	41.0	41.0	37.0	41.0
50-51	39.0567992768279	41.0	41.0	41.0	37.0	41.0
52-53	39.0564445515673	41.0	41.0	41.0	37.0	41.0
54-55	39.04059000266892	41.0	41.0	41.0	37.0	41.0
56-57	39.004391207592306	41.0	41.0	41.0	37.0	41.0
58-59	38.91057140721277	41.0	41.0	41.0	34.5	41.0
60-61	38.94843745161058	41.0	41.0	41.0	37.0	41.0
62-63	38.94642910580933	41.0	41.0	41.0	37.0	41.0
64-65	38.925190057694394	41.0	41.0	41.0	37.0	41.0
66-67	38.883425761596556	41.0	41.0	41.0	32.0	41.0
68-69	38.864183634305135	41.0	41.0	41.0	32.0	41.0
70-71	38.83748736148085	41.0	41.0	41.0	32.0	41.0
72-73	38.80896593396409	41.0	41.0	41.0	32.0	41.0
74-75	38.787127726959504	41.0	41.0	41.0	32.0	41.0
76-77	37.92371974425217	41.0	39.0	41.0	32.0	41.0
78-79	38.377256288116364	41.0	39.0	41.0	32.0	41.0
80-81	38.71161664188935	41.0	41.0	41.0	32.0	41.0
82-83	38.87443165085361	41.0	41.0	41.0	32.0	41.0
84-85	38.8290889154402	41.0	41.0	41.0	32.0	41.0
86-87	38.76703353990698	41.0	41.0	41.0	32.0	41.0
88-89	38.710709008600745	41.0	41.0	41.0	32.0	41.0
90-91	38.653460474970544	41.0	41.0	41.0	32.0	41.0
92-93	38.58671768334857	41.0	41.0	41.0	32.0	41.0
94-95	38.574242463081376	41.0	41.0	41.0	32.0	41.0
96-97	38.47320674535325	41.0	39.0	41.0	32.0	41.0
98-99	38.26993336744202	41.0	37.0	41.0	32.0	41.0
100-101	37.30139195665329	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	24.0
13	124.0
14	427.0
15	1617.0
16	7534.0
17	21130.0
18	36933.0
19	50881.0
20	61751.0
21	74280.0
22	87581.0
23	102949.0
24	119449.0
25	139256.0
26	160843.0
27	185689.0
28	214308.0
29	249345.0
30	288677.0
31	339885.0
32	401176.0
33	478647.0
34	583979.0
35	729441.0
36	953251.0
37	1344128.0
38	2241372.0
39	5693191.0
40	1.9917976E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.587435269309957	20.374816905572764	12.79208514542133	38.24566267969595
2	27.959617285283784	24.445526112105593	31.190754676034604	16.40410192657602
3	20.899314781080555	27.323779691488376	28.904153414943618	22.872752112487447
4	24.29718698489734	30.35615425274208	23.742507215424393	21.604151546936187
5	27.81905746629506	31.861736443131306	22.391603311157525	17.92760277941611
6	22.336730398710845	37.072927662724446	21.972383798987202	18.617958139577503
7	21.754851062946294	21.87820602563765	36.116300357909175	20.250642553506882
8	22.585444044808646	24.361233554266498	28.029416939889884	25.023905461034968
9	23.49117510361643	22.690669829626327	30.293348772325245	23.524806294432
10-11	24.66557085268189	29.94676936406832	23.900387348060562	21.48727243518923
12-13	25.343343435154342	24.88805757482671	26.437707296994855	23.330891693024093
14-15	24.646943540079807	26.616480663776127	26.298386375580655	22.438189420563408
16-17	25.58078386770634	26.089476487222356	25.78131329660017	22.548426348471132
18-19	25.133936519179677	26.786172936779966	25.74457856731229	22.335311976728068
20-21	25.475513035481423	26.647572766279925	25.576312335566993	22.300601862671662
22-23	25.11570338796791	26.441854459593927	25.95438431200247	22.488057840435694
24-25	24.72346897819908	26.514778426188514	26.36223018533744	22.39952241027496
26-27	25.061600638221293	26.601519452445473	26.063244965093503	22.273634944239728
28-29	25.113949212848446	26.340170102450045	26.04742193005095	22.49845875465056
30-31	24.785016135896225	26.35142407997902	26.230632487927508	22.632927296197245
32-33	25.08624123883883	26.563213573509803	25.973275753386094	22.377269434265273
34-35	25.089965900211116	26.528180664506863	25.80911025405091	22.572743181231118
36-37	24.787723043692942	26.54866298183104	26.248038180535758	22.415575793940263
38-39	25.227433403006057	26.639025212091965	26.00904914130881	22.124492243593163
40-41	25.23386842438886	26.324212914725244	25.889488162157203	22.552430498728697
42-43	24.622090612825833	26.546659102725307	26.370696989204045	22.460553295244818
44-45	24.971498179119607	26.548741506434183	26.19728091062926	22.282479403816954
46-47	25.18687868315732	26.303668434005605	26.069445266252565	22.440007616584513
48-49	24.823272945040888	26.41091376050293	26.345944203256906	22.419869091199278
50-51	25.17414826790958	26.388213088245717	26.055920220482353	22.38171842336235
52-53	25.329367505269246	26.27705979011981	25.911982679774038	22.481590024836905
54-55	24.673590094225965	26.41971419747472	26.420762459137187	22.485933249162127
56-57	24.920629995513952	26.530273289393065	26.169317456529264	22.37977925856373
58-59	25.210448669894813	26.23770930472225	26.028793147704317	22.52304887767862
60-61	24.926005836368493	26.44476159888056	26.19906066921171	22.43017189553923
62-63	25.09451861347839	26.379012781942805	26.254405499104504	22.2720631054743
64-65	25.150925695772447	26.27993097689591	26.124285953390036	22.44485737394161
66-67	24.832169234215094	26.437140735477264	26.438004878405813	22.29268515190183
68-69	25.096057239745367	26.644182073444494	26.075450488330116	22.18431019848002
70-71	25.318580256375594	26.353792100269818	25.95180571052688	22.37582193282771
72-73	24.832664672495763	26.492223881775956	26.36339855855058	22.311712887177706
74-75	24.938631531765406	26.647173589611185	26.216969778602017	22.19722510002139
76-77	25.301492008244132	26.33274613507884	26.007562434568737	22.3581994221083
78-79	24.81492405985482	26.623029727792076	26.22972486913761	22.332321343215494
80-81	25.213197648571967	26.593001958576075	26.08576234914179	22.10803804371017
82-83	25.328808784610292	26.22785855369415	26.100542188133808	22.342790473561756
84-85	24.98237780149314	26.524312921296538	26.363680361795303	22.129628915415015
86-87	25.14872189295991	26.768709792922568	25.964421807394363	22.118146506723164
88-89	25.27348882591364	26.389480925086666	26.085063161611327	22.251967087388373
90-91	24.8916743548481	26.62528339174186	26.37890540091943	22.104136852490612
92-93	25.23458588327182	26.703898505229013	26.049059295344513	22.01245631615465
94-95	25.47654626054679	26.475445982995225	25.992692827816537	22.055314928641444
96-97	25.16138322881058	26.536556652058184	26.33257228737971	21.969487831751525
98-99	25.381834287507647	26.792965761567565	25.943229970030885	21.881969980893896
100-101	25.586340326791174	26.458054976927357	25.980279357381086	21.97532533890038
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	29.0
1	23.0
2	22.0
3	37.5
4	54.5
5	67.0
6	80.5
7	97.5
8	106.0
9	138.0
10	190.5
11	241.5
12	283.0
13	343.0
14	436.5
15	553.0
16	687.5
17	853.0
18	1138.0
19	1422.0
20	1779.0
21	2272.5
22	2957.0
23	3830.5
24	4963.5
25	6642.5
26	9066.0
27	12312.5
28	16975.5
29	23332.0
30	32040.5
31	44817.0
32	63938.5
33	91349.5
34	132844.0
35	197330.5
36	288895.0
37	418443.5
38	603168.0
39	847020.5
40	1116817.5
41	1389152.0
42	1665941.5
43	1907267.0
44	2099819.5
45	2250905.0
46	2291010.5
47	2252714.0
48	2173075.0
49	2033822.5
50	1847347.0
51	1663268.0
52	1476092.0
53	1269637.0
54	1087087.5
55	923115.0
56	772348.5
57	630008.0
58	507061.5
59	397822.0
60	326972.0
61	288370.0
62	255676.0
63	214072.0
64	165058.5
65	133617.5
66	116710.0
67	101244.5
68	77606.5
69	57057.0
70	45654.5
71	38755.5
72	31309.5
73	22859.0
74	16135.5
75	10894.0
76	6899.5
77	3971.0
78	2290.0
79	1391.5
80	819.5
81	535.5
82	377.0
83	261.5
84	195.5
85	139.5
86	123.5
87	113.5
88	90.5
89	69.0
90	61.5
91	55.5
92	44.5
93	40.5
94	36.0
95	36.5
96	45.5
97	57.5
98	61.5
99	109.5
100	514.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.042220222303389184
2	0.0
3	2.2328002179676973E-4
4	0.0
5	0.008461442903934727
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.8558339474017223E-4
12-13	4.277117300652407E-4
14-15	0.0
16-17	0.001107700887355403
18-19	3.0447275699559508E-5
20-21	0.0014078240335367754
22-23	4.1321302735116474E-4
24-25	0.002943236650957419
26-27	0.0
28-29	0.0021574069638545025
30-31	0.0
32-33	0.0046874305874607565
34-35	0.0
36-37	0.0
38-39	0.0023313913964234136
40-41	0.0
42-43	8.56873330401889E-4
44-45	9.511148980433827E-4
46-47	0.0016543019796760663
48-49	0.0013396801307806183
50-51	0.0
52-53	0.0031157712132549227
54-55	5.205034274353268E-4
56-57	0.0033463005864087306
58-59	0.0
60-61	4.639584868504306E-5
62-63	0.003195514078182341
64-65	0.0
66-67	0.0023429903585946744
68-69	4.523595246791698E-4
70-71	0.002812748326530735
72-73	0.0
74-75	2.7982496238166597E-4
76-77	0.003427493321607556
78-79	0.0
80-81	0.003921899084157546
82-83	0.001419422995708036
84-85	0.0026967587048181275
86-87	0.0
88-89	9.85911784557165E-4
90-91	3.4796886513782295E-5
92-93	3.668171786661217E-4
94-95	0.0
96-97	0.002458979980307282
98-99	2.029818379970634E-5
100-101	8.829709952872257E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4485844E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.091383546841627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.55961444665338	17.57386123963474
2	14.708832063601795	8.2638288604944
3	6.730729528365891	5.672265141941359
4	3.8190001049583326	4.291239868552517
5	2.489500147916477	3.496675174752035
6	1.7209542510754314	2.9006391560116516
7	1.206786903918767	2.373021964410132
8	0.914594504332277	2.0553780008825195
9	0.7315126898743127	1.8494283186577014
>10	4.486582148599832	23.48210596295857
>50	0.36873709237547375	7.1561665806919725
>100	0.23390342853152946	12.676811799462278
>500	0.020746105320463004	3.978587902546482
>1k	0.008409440271478802	4.0073651342233285
>5k	8.63504041058793E-5	0.16817697405413387
>10k+	1.0793800513234913E-5	0.054447920726405516
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.319792434252153E-5	0.0	5.799481085630382E-6	2.899740542815191E-6	0.0
2	4.639584868504306E-5	1.4498702714075955E-5	1.1598962171260765E-5	2.899740542815191E-6	0.0
3	7.249351357037977E-5	1.4498702714075955E-5	1.1598962171260765E-5	2.899740542815191E-6	0.0
4	1.623854703976507E-4	1.4498702714075955E-5	1.7398443256891148E-5	2.899740542815191E-6	0.0
5	1.681849514832811E-4	1.4498702714075955E-5	2.319792434252153E-5	2.899740542815191E-6	2.899740542815191E-6
6	2.1168105962550895E-4	1.4498702714075955E-5	2.609766488533672E-5	2.899740542815191E-6	2.899740542815191E-6
7	2.3777872451084567E-4	1.4498702714075955E-5	2.609766488533672E-5	2.899740542815191E-6	2.899740542815191E-6
8	2.72575611024628E-4	1.4498702714075955E-5	3.769662705659748E-5	2.899740542815191E-6	5.799481085630382E-6
9	2.9577353536714947E-4	1.4498702714075955E-5	4.059636759941268E-5	2.899740542815191E-6	8.699221628445574E-6
10-11	3.7406653002315963E-4	1.4498702714075955E-5	4.7845718956450655E-5	2.899740542815191E-6	1.1598962171260765E-5
12-13	4.48009913864947E-4	1.4498702714075955E-5	6.37942919419342E-5	2.899740542815191E-6	1.4498702714075955E-5
14-15	5.350021301494028E-4	2.029818379970634E-5	7.829299465601016E-5	8.699221628445574E-6	1.7398443256891148E-5
16-17	6.393927896907497E-4	2.609766488533672E-5	1.2903845415527602E-4	8.699221628445574E-6	1.7398443256891148E-5
18-19	7.756805952030636E-4	2.899740542815191E-5	1.5078650822638993E-4	1.304883244266836E-5	2.319792434252153E-5
20-21	9.322665845150839E-4	4.349610814222787E-5	1.7978391365454184E-4	1.7398443256891148E-5	2.319792434252153E-5
22-23	0.0011192998495266637	4.9295589227858245E-5	2.3777872451084567E-4	1.7398443256891148E-5	2.319792434252153E-5
24-25	0.0014716183254787096	5.3645200042081037E-5	2.4647794613929125E-4	2.029818379970634E-5	2.319792434252153E-5
26-27	0.0019239778501578793	5.944468112771142E-5	2.5082755695351403E-4	2.029818379970634E-5	2.4647794613929123E-5
28-29	0.002493776866821064	6.959377302756459E-5	2.667761299389976E-4	2.029818379970634E-5	3.18971459709671E-5
30-31	0.0031070719916264774	7.249351357037977E-5	2.7692522183885075E-4	2.319792434252153E-5	3.18971459709671E-5
32-33	0.003808809202987753	7.684312438460256E-5	2.8417457319588875E-4	2.319792434252153E-5	3.18971459709671E-5
34-35	0.004587389538733632	7.829299465601016E-5	3.305704218809318E-4	2.4647794613929123E-5	3.18971459709671E-5
36-37	0.00560809820980458	7.974286492741776E-5	3.378197732379698E-4	2.609766488533672E-5	3.18971459709671E-5
38-39	0.006830338848601182	8.554234601304813E-5	3.5811795703767613E-4	2.609766488533672E-5	3.18971459709671E-5
40-41	0.008396198741721386	8.989195682727092E-5	3.8131588138019763E-4	2.609766488533672E-5	3.18971459709671E-5
42-43	0.010296978667536743	8.989195682727092E-5	4.0306393545131157E-4	2.899740542815191E-5	3.18971459709671E-5
44-45	0.012461634982748285	1.0584052981275448E-4	4.1031328680834957E-4	2.899740542815191E-5	3.18971459709671E-5
46-47	0.014820573914328443	1.0729040008416207E-4	4.596088760362078E-4	3.6246756785189886E-5	3.6246756785189886E-5
48-49	0.0179044479816124	1.1598962171260764E-4	4.784571895645065E-4	3.769662705659748E-5	4.059636759941268E-5
50-51	0.02186984317391217	1.2033923252683043E-4	4.8280680037872934E-4	3.769662705659748E-5	4.059636759941268E-5
52-53	0.026677612993899757	1.246888433410532E-4	5.016551139070281E-4	3.769662705659748E-5	4.2046237870820274E-5
54-55	0.0320305340359366	1.246888433410532E-4	5.190535571639192E-4	3.769662705659748E-5	5.3645200042081037E-5
56-57	0.03803009721902123	1.2613871361246082E-4	5.45151222049256E-4	3.769662705659748E-5	5.5095070313488634E-5
58-59	0.045188106748960524	1.304883244266836E-4	5.596499247633318E-4	3.769662705659748E-5	5.654494058489623E-5
60-61	0.054313590237199935	1.36287805512314E-4	6.176447356196357E-4	3.769662705659748E-5	5.799481085630382E-5
62-63	0.06533405417016908	1.4208728659794438E-4	7.031870816326839E-4	3.769662705659748E-5	5.799481085630382E-5
64-65	0.07836548816958054	1.594857298548355E-4	7.524826708605421E-4	3.769662705659748E-5	6.23444216705266E-5
66-67	0.09456633858228902	1.7398443256891146E-4	7.568322816747649E-4	3.769662705659748E-5	6.66940324847494E-5
68-69	0.11335230768891723	1.8848313528298743E-4	7.74230724931656E-4	3.769662705659748E-5	6.66940324847494E-5
70-71	0.13593548703636193	1.8993300555439504E-4	7.887294276457319E-4	3.769662705659748E-5	6.66940324847494E-5
72-73	0.1636613562364894	1.9138287582580262E-4	8.264260547023295E-4	4.059636759941268E-5	6.66940324847494E-5
74-75	0.19910343502104805	1.9138287582580262E-4	8.293257952451447E-4	4.059636759941268E-5	6.66940324847494E-5
76-77	0.24318094114211036	2.0588157853987856E-4	8.322255357879598E-4	4.059636759941268E-5	6.66940324847494E-5
78-79	0.29659416194076615	2.305293731538077E-4	8.365751466021826E-4	4.059636759941268E-5	6.66940324847494E-5
80-81	0.3609640523804492	2.479278164106988E-4	8.438244979592206E-4	4.059636759941268E-5	6.66940324847494E-5
82-83	0.4403966450697857	2.5082755695351403E-4	8.67022422301742E-4	4.059636759941268E-5	6.66940324847494E-5
84-85	0.539431483828553	2.6532625966759E-4	8.757216439301877E-4	4.059636759941268E-5	6.66940324847494E-5
86-87	0.6614844630161871	2.7112574075322036E-4	8.786213844730029E-4	4.2046237870820274E-5	6.66940324847494E-5
88-89	0.8101237133706225	2.856244434672963E-4	8.800712547444105E-4	4.3496108142227864E-5	6.66940324847494E-5
90	0.9359289568206595	3.1027223808122547E-4	8.844208655586333E-4	4.3496108142227864E-5	6.66940324847494E-5
>>END_MODULE
