##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654121_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24804838
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.286856378582275	32.0	32.0	32.0	32.0	32.0
2	31.261602313226152	32.0	32.0	32.0	32.0	32.0
3	34.85210187625495	37.0	32.0	37.0	32.0	37.0
4	35.72692597307025	37.0	37.0	37.0	32.0	37.0
5	35.99031696961698	37.0	37.0	37.0	37.0	37.0
6	38.73588797475718	41.0	37.0	41.0	32.0	41.0
7	39.01697886517138	41.0	41.0	41.0	37.0	41.0
8	39.28950884500838	41.0	41.0	41.0	37.0	41.0
9	39.62343640381768	41.0	41.0	41.0	37.0	41.0
10-11	39.598574822379405	41.0	41.0	41.0	37.0	41.0
12-13	39.4831880175956	41.0	41.0	41.0	37.0	41.0
14-15	39.37639828568927	41.0	41.0	41.0	37.0	41.0
16-17	39.45686557195012	41.0	41.0	41.0	37.0	41.0
18-19	39.491604420073216	41.0	41.0	41.0	37.0	41.0
20-21	39.378565826553675	41.0	41.0	41.0	37.0	41.0
22-23	39.27871965944708	41.0	41.0	41.0	37.0	41.0
24-25	39.309613672945574	41.0	41.0	41.0	37.0	41.0
26-27	39.12604212533056	41.0	41.0	41.0	37.0	41.0
28-29	39.283505358914255	41.0	41.0	41.0	37.0	41.0
30-31	39.27806083635781	41.0	41.0	41.0	37.0	41.0
32-33	39.28943639946368	41.0	41.0	41.0	37.0	41.0
34-35	39.08453294474248	41.0	41.0	41.0	37.0	41.0
36-37	39.009044828271	41.0	41.0	41.0	37.0	41.0
38-39	38.97886277265749	41.0	41.0	41.0	37.0	41.0
40-41	38.92739545406424	41.0	41.0	41.0	37.0	41.0
42-43	38.918476448021956	41.0	41.0	41.0	37.0	41.0
44-45	38.9241880354147	41.0	41.0	41.0	37.0	41.0
46-47	38.881564435131565	41.0	41.0	41.0	34.5	41.0
48-49	38.840979731454	41.0	41.0	41.0	32.0	41.0
50-51	38.90386510486382	41.0	41.0	41.0	32.0	41.0
52-53	38.910703488569446	41.0	41.0	41.0	32.0	41.0
54-55	38.904201793214696	41.0	41.0	41.0	32.0	41.0
56-57	38.86031704782752	41.0	41.0	41.0	32.0	41.0
58-59	38.77327148840884	41.0	41.0	41.0	32.0	41.0
60-61	38.818990613847184	41.0	41.0	41.0	32.0	41.0
62-63	38.81305324388734	41.0	41.0	41.0	32.0	41.0
64-65	38.7865604484093	41.0	41.0	41.0	32.0	41.0
66-67	38.74413638581312	41.0	41.0	41.0	32.0	41.0
68-69	38.7247964288257	41.0	41.0	41.0	32.0	41.0
70-71	38.702202167174	41.0	41.0	41.0	32.0	41.0
72-73	38.6691906836884	41.0	41.0	41.0	32.0	41.0
74-75	38.64775252714813	41.0	41.0	41.0	32.0	41.0
76-77	37.75675307776652	41.0	39.0	41.0	29.5	41.0
78-79	38.22612498013493	41.0	37.0	41.0	32.0	41.0
80-81	38.582133654733	41.0	41.0	41.0	32.0	41.0
82-83	38.75325095854284	41.0	41.0	41.0	32.0	41.0
84-85	38.7106681164376	41.0	41.0	41.0	32.0	41.0
86-87	38.645904863398016	41.0	41.0	41.0	32.0	41.0
88-89	38.590604320818386	41.0	41.0	41.0	32.0	41.0
90-91	38.53606699628516	41.0	41.0	41.0	32.0	41.0
92-93	38.4665843413289	41.0	39.0	41.0	32.0	41.0
94-95	38.45964142156461	41.0	37.0	41.0	32.0	41.0
96-97	38.35837938147389	41.0	37.0	41.0	32.0	41.0
98-99	38.14724454156887	41.0	37.0	41.0	32.0	41.0
100-101	37.14558079355326	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	25.0
13	69.0
14	256.0
15	1122.0
16	5366.0
17	15178.0
18	26757.0
19	36645.0
20	45474.0
21	54276.0
22	65015.0
23	76613.0
24	90494.0
25	105189.0
26	121203.0
27	141489.0
28	163733.0
29	189615.0
30	222169.0
31	261965.0
32	309228.0
33	370519.0
34	452601.0
35	570748.0
36	748494.0
37	1065951.0
38	1815206.0
39	4700034.0
40	1.3149404E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.481720858402944	18.278923806496636	13.039032141974602	38.20032319312582
2	28.61852998193336	20.71452754498941	33.69756738584626	16.96937508723097
3	22.11142678513634	23.58520771728924	28.96549502652151	25.33787047105291
4	25.181385986072556	29.79416757327744	22.88276988545541	22.141676555194596
5	27.83718915266319	31.6545490685113	21.979238609235985	18.529023169589525
6	21.77520772359005	35.122837730284715	22.33319161366827	20.768762932456966
7	21.77022482468944	18.87916381473646	37.25706251336936	22.093548847204726
8	22.859298657786034	23.017384753732316	26.915833919173348	27.207482669308302
9	23.48261254518171	22.500658944033418	29.215893286624166	24.800835224160707
10-11	26.16493529145699	28.233387726702336	22.32092332288663	23.28075365895404
12-13	26.99095264103119	22.652721489561326	24.757195218940403	25.59913065046708
14-15	24.917340721999476	26.420970779974457	25.88264837690131	22.77904012112476
16-17	26.318472869554327	25.855409324089933	24.876997548880777	22.94912025747496
18-19	25.943564664005077	26.627828534130536	24.764227888425687	22.6643789134387
20-21	26.12843257540552	26.673944104616105	24.68019836870841	22.517424951269966
22-23	25.871388649922533	26.549506834316833	24.865756542487613	22.713347973273017
24-25	25.220593295528072	26.59809569623705	25.435761083506613	22.745549924728266
26-27	25.653041958992034	26.42969085305052	25.24766741068819	22.669599777269255
28-29	26.06298532630429	26.291058528239258	24.987966812389956	22.657989333066496
30-31	25.605025116471232	26.528844897112407	25.12211932204516	22.7440106643712
32-33	25.661528997687384	26.617015727919714	25.01881221843029	22.70264305596261
34-35	25.9091210351787	26.29291108452311	24.987157747210443	22.81081013308775
36-37	25.767092290624916	26.306537458539335	25.156269917989388	22.770100332846358
38-39	25.834917484544256	26.632154495033472	24.873273446521406	22.659654573900866
40-41	26.067322028065654	26.284692929661546	24.726236873629247	22.921748168643553
42-43	25.809914673256827	26.314703089944828	25.09833035338132	22.77705188341702
44-45	25.982743819845982	26.34735532438402	25.151713165698304	22.518187690071695
46-47	25.957809620948407	26.374269226285698	25.056999356949973	22.610921795815926
48-49	25.510197170452102	26.50691236696784	25.212566028267098	22.77032443431296
50-51	26.02698312321169	26.391537005805077	24.947937575726154	22.63354229525708
52-53	26.295457395975667	26.194891267270297	24.917704053248986	22.591947283505046
54-55	25.83564122705014	26.352508497978043	25.10928966595234	22.702560609019475
56-57	25.499384777144996	26.563811027406043	25.38803992256094	22.548764272888025
58-59	26.009424048647283	26.057402592187863	25.230166792462015	22.703006566702836
60-61	26.01007053989935	26.056537302195252	25.10911485091188	22.824277306993515
62-63	25.913144415459264	26.338304677035012	25.087094114053954	22.661456793451766
64-65	26.028214737786232	26.287073513642785	24.974061511709934	22.710650236861053
66-67	25.80624704347678	26.49025450727489	25.227923032499877	22.47557541674845
68-69	26.058682637168744	26.6154433727873	25.109307275078034	22.21656671496592
70-71	26.291888343227644	26.383949528458057	24.7180653919448	22.6060967363695
72-73	25.694040009452994	26.641897439523692	25.089252749806306	22.574809801217004
74-75	25.639574410992466	26.48670764049633	25.213583867880928	22.66013408063028
76-77	26.083906083834723	26.32362755772316	25.092739096776107	22.499727261666006
78-79	25.890298094266935	26.4690581732483	25.2648959045812	22.375747827903574
80-81	25.829606495245017	26.608281418043344	25.226559422103033	22.335552664608603
82-83	26.188554159928223	26.28157963214452	25.122838111227132	22.40702809670013
84-85	26.040327111781536	26.248515799644505	25.19509865705506	22.516058431518896
86-87	25.789321825040744	26.62067980448008	25.231089999458977	22.358908371020203
88-89	26.00849741151973	26.496043830557724	25.073393103802772	22.422065654119773
90-91	25.786179609872722	26.49891988769283	25.32940966169973	22.385490840734715
92-93	25.996945303861757	26.55993607033102	25.1762559994285	22.266862626378725
94-95	26.274285685719857	26.298440247825848	25.07170980112831	22.35556426532598
96-97	25.916602188526234	26.404391146815353	25.33720401066397	22.341802653994442
98-99	25.93395803681016	26.68554739428825	25.203209374301423	22.177285194600167
100-101	26.346863251439228	26.40261896224714	25.224012577649805	22.02650520866383
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15.0
1	13.5
2	11.5
3	13.0
4	21.0
5	30.5
6	38.0
7	48.5
8	66.0
9	75.0
10	80.5
11	110.0
12	139.5
13	171.0
14	214.0
15	277.5
16	361.5
17	476.5
18	589.0
19	717.5
20	916.5
21	1168.5
22	1570.0
23	2103.0
24	2829.0
25	3786.5
26	5008.0
27	6897.0
28	9978.5
29	13339.0
30	17933.5
31	24997.5
32	35521.0
33	49763.5
34	70557.0
35	106202.0
36	158533.5
37	223403.0
38	315440.0
39	447595.0
40	608047.5
41	789060.5
42	989685.5
43	1180287.0
44	1347913.5
45	1477053.5
46	1550697.5
47	1593255.0
48	1581795.5
49	1490918.5
50	1367078.5
51	1260789.0
52	1167331.0
53	1059407.5
54	934393.5
55	810093.5
56	683711.0
57	567330.0
58	463100.0
59	383759.5
60	330668.5
61	281722.0
62	233039.0
63	187961.0
64	161898.5
65	146073.5
66	128761.5
67	109867.5
68	88027.5
69	67808.5
70	64016.0
71	59229.0
72	45403.5
73	34711.5
74	23102.5
75	13716.5
76	8854.0
77	4819.0
78	2712.5
79	1564.5
80	914.0
81	561.5
82	366.0
83	274.0
84	215.0
85	173.0
86	141.5
87	129.5
88	114.0
89	84.5
90	77.0
91	71.5
92	51.0
93	46.0
94	44.5
95	40.0
96	39.5
97	35.0
98	38.5
99	79.0
100	365.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.041826517875262884
2	0.0
3	2.6607712576070847E-4
4	0.0
5	0.008337083273835533
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.511801850913116E-4
12-13	3.446908220081905E-4
14-15	0.0
16-17	0.0010118993722111791
18-19	2.6204565415827346E-5
20-21	0.0012941023843816275
22-23	3.6686391582158284E-4
24-25	0.0028502504229215284
26-27	0.0
28-29	0.0021003967048686227
30-31	0.0
32-33	0.004614019248986831
34-35	0.0
36-37	0.0
38-39	0.0021991677591282795
40-41	0.0
42-43	7.579166612577756E-4
44-45	8.768450735296073E-4
46-47	0.0015783211323532934
48-49	0.0012497561967548428
50-51	0.0
52-53	0.0030679498894530177
54-55	4.5958776267758733E-4
56-57	0.0032977437707918107
58-59	0.0
60-61	4.434618762678475E-5
62-63	0.0031425321140980643
64-65	0.0
66-67	0.002324143378803764
68-69	4.3338319726176E-4
70-71	0.0027494636328606542
72-73	0.0
74-75	2.4995123935096856E-4
76-77	0.0034025620324551202
78-79	0.0
80-81	0.003910527454361928
82-83	0.0013767475522315446
84-85	0.002646661106998562
86-87	0.0
88-89	9.090968463490872E-4
90-91	4.0314716024349764E-5
92-93	3.2050199239358067E-4
94-95	0.0
96-97	0.0022818129269781967
98-99	1.2094414807304929E-5
100-101	8.224202068967352E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.4804838E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.17159775944405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.48496044370842	18.85271092522343
2	15.520000653480015	9.365264338862158
3	6.9711809262864675	6.3099500044867165
4	3.843104915138857	4.638104625876479
5	2.4088219369283737	3.6339003277563906
6	1.6292362007150107	2.9493995581858896
7	1.147004426133141	2.422486932151376
8	0.8499948078351374	2.0516561151694166
9	0.646132675085385	1.7545369654718812
>10	3.9501468919557152	22.287168302726695
>50	0.32605671623465526	6.80849651267258
>100	0.2008972191253029	11.56445102586805
>500	0.015065558254693842	3.0470720508227918
>1k	0.007090398148554769	3.5546116916386086
>5k	2.3663302236115151E-4	0.4836698560202477
>10k+	6.959794775327985E-5	0.27652076706740836
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4188829614609858E-5	0.0	8.062943204869953E-6	4.031471602434976E-6	0.0
2	2.8220301217044835E-5	4.031471602434976E-6	1.2094414807304929E-5	4.031471602434976E-6	0.0
3	4.434618762678474E-5	4.031471602434976E-6	2.0157358012174882E-5	4.031471602434976E-6	2.0157358012174882E-5
4	7.256648884382957E-5	4.031471602434976E-6	5.240913083165469E-5	4.031471602434976E-6	2.0157358012174882E-5
5	8.062943204869953E-5	4.031471602434976E-6	8.062943204869953E-5	4.031471602434976E-6	2.0157358012174882E-5
6	1.0884973326574436E-4	4.031471602434976E-6	1.2497561967548428E-4	4.031471602434976E-6	2.0157358012174882E-5
7	1.2497561967548428E-4	4.031471602434976E-6	1.531959208925291E-4	4.031471602434976E-6	2.0157358012174882E-5
8	1.4513297768765915E-4	4.031471602434976E-6	1.69321807302269E-4	4.031471602434976E-6	2.4188829614609858E-5
9	1.69321807302269E-4	4.031471602434976E-6	1.73353278904704E-4	4.031471602434976E-6	2.8220301217044835E-5
10-11	1.874634295132264E-4	8.062943204869953E-6	1.8947916531444388E-4	4.031471602434976E-6	3.225177281947981E-5
12-13	2.3785682454366362E-4	1.2094414807304929E-5	2.056050517241838E-4	6.0472074036524646E-6	3.225177281947981E-5
14-15	3.0236037018262327E-4	1.6125886409739905E-5	2.60029918357056E-4	8.062943204869953E-6	3.42675086206973E-5
16-17	4.212887824544551E-4	1.6125886409739905E-5	4.636192342800223E-4	8.062943204869953E-6	3.628324442191479E-5
18-19	5.785161749494192E-4	1.6125886409739905E-5	4.8780806389463215E-4	1.2094414807304929E-5	3.628324442191479E-5
20-21	7.780740192699505E-4	1.6125886409739905E-5	5.603745527384618E-4	1.2094414807304929E-5	3.628324442191479E-5
22-23	0.0010965602758623136	1.6125886409739905E-5	6.490669279920312E-4	1.4110150608522417E-5	3.628324442191479E-5
24-25	0.001535990680527726	1.6125886409739905E-5	6.833344366127285E-4	1.6125886409739905E-5	4.0314716024349764E-5
26-27	0.0021165225912783627	1.6125886409739905E-5	7.075232662273384E-4	1.6125886409739905E-5	4.0314716024349764E-5
28-29	0.0028764549883373556	1.6125886409739905E-5	7.498537180529056E-4	1.6125886409739905E-5	4.233045182556725E-5
30-31	0.003737174175457223	1.6125886409739905E-5	7.921841698784729E-4	1.6125886409739905E-5	5.240913083165469E-5
32-33	0.004575720268763699	1.6125886409739905E-5	8.163729994930828E-4	1.6125886409739905E-5	5.240913083165469E-5
34-35	0.005539241981745657	1.8141622210957394E-5	0.0010723714462477039	1.6125886409739905E-5	5.240913083165469E-5
36-37	0.006662006823023798	2.0157358012174882E-5	0.001118733369675706	1.6125886409739905E-5	6.0472074036524646E-5
38-39	0.00795409347160421	2.0157358012174882E-5	0.0011409064634890984	1.6125886409739905E-5	6.0472074036524646E-5
40-41	0.009594902413795244	2.0157358012174882E-5	0.0012235516313390154	1.6125886409739905E-5	6.0472074036524646E-5
42-43	0.011604591007609081	2.0157358012174882E-5	0.0013102282707913673	1.6125886409739905E-5	6.0472074036524646E-5
44-45	0.014009363818461544	2.4188829614609858E-5	0.0013424800436108472	1.6125886409739905E-5	6.651928144017712E-5
46-47	0.01648468738235662	2.4188829614609858E-5	0.0014311724188644168	1.6125886409739905E-5	7.659796044626455E-5
48-49	0.019232135279416057	2.4188829614609858E-5	0.0014956759645033764	1.6125886409739905E-5	7.861369624748203E-5
50-51	0.022947136361059887	2.4188829614609858E-5	0.0015158333225155512	1.6125886409739905E-5	8.062943204869953E-5
52-53	0.02745633734838341	2.4188829614609858E-5	0.0016025099619679031	1.6125886409739905E-5	9.272384685600446E-5
54-55	0.03268717175254279	2.6204565415827346E-5	0.0017516744112579972	1.6125886409739905E-5	1.0481826166330939E-4
56-57	0.03833929493915662	2.8220301217044835E-5	0.0019250276901627015	1.6125886409739905E-5	1.0481826166330939E-4
58-59	0.044271605402139695	2.8220301217044835E-5	0.0019713896135907035	1.6125886409739905E-5	1.0683399746452688E-4
60-61	0.051268224368165596	2.8220301217044835E-5	0.002084270818458883	1.6125886409739905E-5	1.1086546906696184E-4
62-63	0.0603853973970723	3.0236037018262323E-5	0.0022294037961465424	1.6125886409739905E-5	1.2094414807304929E-4
64-65	0.07114337936817003	3.628324442191479E-5	0.0022898758701830665	1.6125886409739905E-5	1.3102282707913674E-4
66-67	0.08314910180022139	4.233045182556725E-5	0.002303986020791589	1.6125886409739905E-5	1.3303856288035423E-4
68-69	0.09712621384586345	4.434618762678474E-5	0.0023422850010147212	1.8141622210957394E-5	1.370700344827892E-4
70-71	0.11350205149495433	6.248780983774213E-5	0.0023543794158220263	2.4188829614609858E-5	1.5118018509131163E-4
72-73	0.13356265418867078	6.450354563895962E-5	0.002402757075051246	2.8220301217044835E-5	1.552116566937466E-4
74-75	0.15816874111413265	6.450354563895962E-5	0.002402757075051246	3.225177281947981E-5	1.73353278904704E-4
76-77	0.1886386034853362	6.450354563895962E-5	0.002424930168864638	3.225177281947981E-5	1.854476937120089E-4
78-79	0.22558704072165275	7.659796044626455E-5	0.002449118998479248	3.628324442191479E-5	1.854476937120089E-4
80-81	0.2701650379655775	8.869237525356948E-5	0.002475323563895076	3.628324442191479E-5	1.9351063691687887E-4
82-83	0.32333410119429123	8.869237525356948E-5	0.0025055596009133383	4.0314716024349764E-5	1.9754210851931386E-4
84-85	0.3892970395533323	8.869237525356948E-5	0.0025277326947267306	4.0314716024349764E-5	1.9754210851931386E-4
86-87	0.4728130052693753	8.869237525356948E-5	0.0025378113737328178	4.0314716024349764E-5	1.9754210851931386E-4
88-89	0.5750833768799457	9.272384685600446E-5	0.0025660316749498624	4.0314716024349764E-5	2.056050517241838E-4
90	0.6646525972070448	1.0078679006087441E-4	0.002572078882353515	4.0314716024349764E-5	2.056050517241838E-4
>>END_MODULE
