##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654141_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24414141
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.65412397675593	32.0	32.0	32.0	32.0	32.0
2	31.68507001741327	32.0	32.0	32.0	32.0	32.0
3	35.80241352747164	37.0	37.0	37.0	32.0	37.0
4	36.385731613494	37.0	37.0	37.0	37.0	37.0
5	36.563735623547025	37.0	37.0	37.0	37.0	37.0
6	40.16640876285592	41.0	41.0	41.0	37.0	41.0
7	40.18625652239823	41.0	41.0	41.0	37.0	41.0
8	40.223136992614236	41.0	41.0	41.0	41.0	41.0
9	40.284115627905976	41.0	41.0	41.0	41.0	41.0
10-11	40.32682538369873	41.0	41.0	41.0	41.0	41.0
12-13	40.31938266842974	41.0	41.0	41.0	41.0	41.0
14-15	40.31469145279369	41.0	41.0	41.0	41.0	41.0
16-17	40.31252995958367	41.0	41.0	41.0	41.0	41.0
18-19	40.308823767340414	41.0	41.0	41.0	41.0	41.0
20-21	40.275471662099434	41.0	41.0	41.0	41.0	41.0
22-23	40.28105987837131	41.0	41.0	41.0	41.0	41.0
24-25	40.27011454140451	41.0	41.0	41.0	41.0	41.0
26-27	40.19033600240123	41.0	41.0	41.0	41.0	41.0
28-29	40.20085400506207	41.0	41.0	41.0	41.0	41.0
30-31	40.114006407188356	41.0	41.0	41.0	41.0	41.0
32-33	40.140761659400596	41.0	41.0	41.0	41.0	41.0
34-35	40.149923562741776	41.0	41.0	41.0	41.0	41.0
36-37	40.14001612835774	41.0	41.0	41.0	41.0	41.0
38-39	40.06659195996288	41.0	41.0	41.0	37.0	41.0
40-41	40.08770945494253	41.0	41.0	41.0	37.0	41.0
42-43	40.07310707757443	41.0	41.0	41.0	37.0	41.0
44-45	40.06782870222631	41.0	41.0	41.0	37.0	41.0
46-47	40.05665351895854	41.0	41.0	41.0	37.0	41.0
48-49	39.96583043818744	41.0	41.0	41.0	37.0	41.0
50-51	39.92492703306661	41.0	41.0	41.0	37.0	41.0
52-53	39.96852748167548	41.0	41.0	41.0	37.0	41.0
54-55	39.937616871304215	41.0	41.0	41.0	37.0	41.0
56-57	39.92836794872284	41.0	41.0	41.0	37.0	41.0
58-59	39.92793746050701	41.0	41.0	41.0	37.0	41.0
60-61	39.91057348689843	41.0	41.0	41.0	37.0	41.0
62-63	39.866399620613315	41.0	41.0	41.0	37.0	41.0
64-65	39.810539985822146	41.0	41.0	41.0	37.0	41.0
66-67	39.78605589686731	41.0	41.0	41.0	37.0	41.0
68-69	39.76610743748879	41.0	41.0	41.0	37.0	41.0
70-71	39.74362857984641	41.0	41.0	41.0	37.0	41.0
72-73	39.72130231819338	41.0	41.0	41.0	37.0	41.0
74-75	39.69880373837441	41.0	41.0	41.0	37.0	41.0
76-77	39.157028092858155	41.0	39.0	41.0	34.5	41.0
78-79	39.49233915295238	41.0	41.0	41.0	37.0	41.0
80-81	39.67659415090623	41.0	41.0	41.0	37.0	41.0
82-83	39.747657740651206	41.0	41.0	41.0	37.0	41.0
84-85	39.78604272826965	41.0	41.0	41.0	37.0	41.0
86-87	39.78764382904154	41.0	41.0	41.0	37.0	41.0
88-89	39.66853419090191	41.0	41.0	41.0	37.0	41.0
90-91	39.68910929530554	41.0	41.0	41.0	37.0	41.0
92-93	39.63652837509213	41.0	41.0	41.0	37.0	41.0
94-95	39.62339113221309	41.0	41.0	41.0	37.0	41.0
96-97	39.58719540859537	41.0	41.0	41.0	37.0	41.0
98-99	39.53646997041592	41.0	41.0	41.0	37.0	41.0
100-101	39.006685654023215	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	1.0
15	0.0
16	3.0
17	16.0
18	41.0
19	120.0
20	373.0
21	1099.0
22	2723.0
23	5844.0
24	11319.0
25	19865.0
26	32637.0
27	50196.0
28	73290.0
29	100437.0
30	132470.0
31	167182.0
32	206108.0
33	250996.0
34	302090.0
35	371163.0
36	472340.0
37	642950.0
38	1005787.0
39	2387571.0
40	1.8177519E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.309678940271866	6.597017542256226	5.996612805134326	51.096690712337576
2	19.367337151038818	7.576092888133972	37.59022281390118	35.46634714692603
3	18.73186961812688	9.323236175116623	22.914717829388145	49.03017637736835
4	23.694636645213116	15.933528031971306	21.81822821454173	38.553607108273845
5	26.16166589682594	21.840453039080916	25.714490630655405	26.283390433437738
6	22.342502240811996	27.639743704273684	25.83946738081016	24.17828667410416
7	16.298316619044677	23.995257502608837	41.46669342165264	18.239732456693847
8	18.328320459851525	23.48571674096582	34.66515164305801	23.52081115612464
9	18.803745747188074	21.25366606181229	36.8512248700456	23.091363320954034
10-11	20.96946381237274	30.701258894460988	27.303050968643994	21.026226324522277
12-13	21.461627505141383	25.036952969182902	29.715313350570067	23.786106175105648
14-15	21.11926444596187	25.733575881289454	28.96923754147238	24.177922131276297
16-17	21.931639880632318	25.841871345728677	27.692688529715586	24.533800243923416
18-19	21.711134339461612	25.85521511262571	27.256992224801422	25.17665832311125
20-21	21.89367833994241	25.727399133149927	27.55628182863366	24.822640698274004
22-23	21.843023270816698	26.033539332798973	27.4756748558141	24.647762540570238
24-25	21.82502395183374	25.76031110544863	27.09794788065414	25.3167170620635
26-27	21.643198505325252	25.89209466759449	27.464281868446648	25.000424958633605
28-29	21.80007925348128	26.043982548669327	27.175752080168365	24.980186117681036
30-31	21.723226585601786	25.78112507012457	27.210326941074385	25.28532140319925
32-33	21.764957857825102	25.594627720057815	27.391137783631216	25.249276638485867
34-35	21.911174347686448	25.781771310323798	26.997783784405932	25.30927055758382
36-37	21.80827701453842	25.645995490891938	26.917545450401057	25.628182044168586
38-39	21.890129400158038	25.89369620371848	27.287013917386666	24.92916047873682
40-41	22.05747890126464	25.98221456982656	27.050759230070803	24.909547298837996
42-43	22.02394096110119	25.61211553582819	26.98080387100247	25.38313963206815
44-45	21.856732538736466	25.592581774636265	27.38383668710687	25.166848999520404
46-47	21.90098844435779	25.80057480221589	27.160815798889264	25.13762095453705
48-49	21.980548076624938	25.580998323881225	27.070600190274973	25.36785340921886
50-51	21.81322961692944	25.620780221705363	27.273275026580308	25.29271513478489
52-53	22.33179201931415	25.896199302228716	26.887504829705	24.884503848752132
54-55	22.26424226844598	25.61111201905486	26.821168928286276	25.303476784212886
56-57	22.107708975644204	25.551041722496226	27.309523093208526	25.031726208651044
58-59	22.155867828552246	25.708893398849554	27.082969814010816	25.052268958587387
60-61	21.842976376350787	25.413153097472925	27.218299735172984	25.5255707910033
62-63	22.001107058387102	25.461546207931796	27.42321090637861	25.114135827302498
64-65	22.234014582257515	25.687209048416825	27.00844581109389	25.070330558231767
66-67	22.10872128351498	25.465287288243026	27.140505437579705	25.28548599066228
68-69	22.10680484993672	25.48017994801805	27.295722600393574	25.11729260165166
70-71	22.212039047085824	25.777533208631194	27.071635681119314	24.938792063163667
72-73	22.214328572936477	25.59828953228377	26.816034608794958	25.37134728598479
74-75	22.0718169031628	25.604900455027273	27.162575574541	25.160707067268923
76-77	22.120738341059774	25.709135660807235	27.03161419132102	25.13851180681197
78-79	22.081107505732334	25.464261694247675	27.05082177787135	25.40380902214864
80-81	22.143773768293293	25.49470644458558	27.23242575053826	25.129094036582867
82-83	22.113671369843644	25.872629603882253	26.843246612619755	25.17045241365435
84-85	22.321900816883332	25.46961217343969	26.8253800513514	25.383106958325573
86-87	22.187102396987125	25.572353265064102	27.152729016519395	25.087815321429378
88-89	22.392784575136186	25.761012849069726	26.997171434374856	24.84903114141923
90-91	22.270639866790752	25.5217185013587	26.888189320383677	25.319452311466872
92-93	22.243750534020737	25.619978105866647	27.056838975718943	25.079432384393673
94-95	22.51957379946319	25.84525910618768	26.740996130070684	24.894170964278448
96-97	22.49739944975978	25.84206996426322	26.556572064364214	25.10395852161279
98-99	22.386230913163896	26.113033756230607	26.81043097322407	24.690304357381425
100-101	22.453773819033813	26.177804084935858	26.70329666728803	24.6651254287423
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	27.5
2	14.5
3	12.0
4	23.5
5	39.0
6	54.0
7	64.0
8	68.5
9	71.5
10	76.5
11	94.5
12	113.0
13	137.0
14	158.5
15	192.5
16	263.0
17	332.0
18	424.5
19	579.5
20	827.5
21	1153.0
22	1600.0
23	2217.5
24	3633.0
25	5331.5
26	7011.5
27	9892.0
28	14161.0
29	20141.0
30	28203.0
31	38813.0
32	53723.5
33	73841.0
34	104324.0
35	150154.5
36	205030.0
37	272414.5
38	370156.5
39	510192.5
40	683005.5
41	874977.0
42	1072459.0
43	1266198.0
44	1438775.5
45	1556851.0
46	1630521.5
47	1657056.5
48	1603470.5
49	1498115.5
50	1379630.0
51	1247338.0
52	1114669.0
53	981361.0
54	833998.0
55	690123.0
56	562867.0
57	441897.5
58	341343.0
59	272852.5
60	215972.5
61	179050.0
62	154998.5
63	129180.0
64	116881.0
65	105217.0
66	96953.5
67	87237.5
68	61505.0
69	44361.0
70	43228.5
71	40949.0
72	32693.0
73	31438.5
74	26348.5
75	14836.0
76	7302.0
77	2144.5
78	495.5
79	151.5
80	48.5
81	21.5
82	9.5
83	6.0
84	3.5
85	1.5
86	2.0
87	1.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.5
93	1.0
94	2.5
95	2.0
96	0.0
97	0.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059801407716945684
2	0.0
3	4.505585512920565E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.75486149604854E-4
12-13	0.0
14-15	0.0
16-17	0.0013537236472911333
18-19	0.00199884157300476
20-21	0.0
22-23	0.0
24-25	1.6179147978214757E-4
26-27	0.0
28-29	2.9286305833983674E-4
30-31	0.001822714139317865
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.002807798971915498
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0021544890725420157
48-49	0.0
50-51	0.0015892428900119813
52-53	5.079023669110455E-4
54-55	0.0
56-57	7.270376623121821E-4
58-59	1.3516756538761696E-4
60-61	0.0011448283189648162
62-63	0.003254261536377627
64-65	0.0020479934149638932
66-67	0.0010076127601622354
68-69	3.7068680810846465E-4
70-71	9.502689445432464E-4
72-73	0.0
74-75	0.0
76-77	0.001013756740407127
78-79	4.095986829927786E-6
80-81	0.0018247621327328288
82-83	0.0011837401938491303
84-85	2.4575920979566723E-5
86-87	0.0010792925296859719
88-89	0.0
90-91	0.002131961144977413
92-93	0.001208316114828697
94-95	0.0
96-97	3.153909859044396E-4
98-99	0.0011980761477538775
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.4414141E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.524933287436575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.199325153121094	14.906833504854934
2	17.69434406331458	9.386825916887373
3	7.915723722682863	6.29892130997826
4	4.262782733643285	4.522801105148986
5	2.7216519296656774	3.609581793300256
6	1.9718148855153557	3.1381354976081512
7	1.3847506530022664	2.571129308343354
8	1.0621444881347353	2.2538649351513858
9	0.7720828251385534	1.8431500886258119
>10	5.323514664449472	26.550017658177154
>50	0.4400244059007044	8.026666308746343
>100	0.23213074657067462	11.356996078104618
>500	0.013872196677868284	2.4539243281943772
>1k	0.005447506440393396	2.405610034976434
>5k	3.737746696791581E-4	0.6285524321261708
>10k+	1.6251072594746004E-5	0.04698969977648124
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.8671907809494505E-5	0.0	4.095986829927786E-6	0.0	2.8671907809494505E-5
2	2.8671907809494505E-5	0.0	4.095986829927786E-6	0.0	2.8671907809494505E-5
3	3.6863881469350076E-5	0.0	4.095986829927786E-6	0.0	2.8671907809494505E-5
4	4.5055855129205654E-5	0.0	8.191973659855573E-6	0.0	2.8671907809494505E-5
5	6.553578927884458E-5	0.0	1.6383947319711145E-5	0.0	2.8671907809494505E-5
6	9.830368391826688E-5	0.0	2.8671907809494505E-5	0.0	2.8671907809494505E-5
7	1.0649565757812245E-4	0.0	2.8671907809494505E-5	0.0	2.8671907809494505E-5
8	1.3107157855768916E-4	0.0	2.8671907809494505E-5	0.0	2.8671907809494505E-5
9	1.3516756538761696E-4	4.095986829927786E-6	2.8671907809494505E-5	0.0	2.8671907809494505E-5
10-11	1.5769549295221978E-4	1.6383947319711145E-5	2.8671907809494505E-5	0.0	3.4815888054386183E-5
12-13	2.0275134808142543E-4	1.0035167733323078E-4	2.8671907809494505E-5	2.047993414963893E-6	6.758378269380847E-5
14-15	2.990070385847284E-4	1.0444766416315855E-4	2.8671907809494505E-5	4.095986829927786E-6	7.577575635366404E-5
16-17	3.420149002989702E-4	1.0649565757812245E-4	3.07199012244584E-5	4.095986829927786E-6	7.782374976862794E-5
18-19	4.1369466982270646E-4	1.0649565757812245E-4	3.276789463942229E-5	4.095986829927786E-6	7.782374976862794E-5
20-21	5.201903274008289E-4	1.0649565757812245E-4	3.276789463942229E-5	4.095986829927786E-6	7.782374976862794E-5
22-23	6.184940113190958E-4	1.1263963782301412E-4	3.276789463942229E-5	4.095986829927786E-6	8.191973659855574E-5
24-25	7.29085655727146E-4	1.249275983127975E-4	3.276789463942229E-5	4.095986829927786E-6	8.191973659855574E-5
26-27	8.847331552644019E-4	1.9455937442156986E-4	3.276789463942229E-5	4.095986829927786E-6	8.191973659855574E-5
28-29	0.0010506206218764773	1.9660736783653376E-4	3.276789463942229E-5	4.095986829927786E-6	8.191973659855574E-5
30-31	0.001304571805332	1.9660736783653376E-4	4.5055855129205654E-5	4.095986829927786E-6	8.191973659855574E-5
32-33	0.001632250751726223	2.0070335466646153E-4	5.734381561898901E-5	4.095986829927786E-6	8.191973659855574E-5
34-35	0.0018923459154266374	2.0479934149638933E-4	5.734381561898901E-5	4.095986829927786E-6	8.191973659855574E-5
36-37	0.002279416670854813	2.1503930857120877E-4	5.734381561898901E-5	4.095986829927786E-6	8.191973659855574E-5
38-39	0.002904054662418801	2.2118328881610047E-4	5.734381561898901E-5	4.095986829927786E-6	9.830368391826688E-5
40-41	0.0036945801205948633	2.2118328881610047E-4	5.734381561898901E-5	4.095986829927786E-6	1.0649565757812245E-4
42-43	0.004696048900512208	2.416632229657394E-4	5.734381561898901E-5	4.095986829927786E-6	1.1059164440805024E-4
44-45	0.005793773370932854	2.8876707150990894E-4	5.734381561898901E-5	4.095986829927786E-6	1.187836180679058E-4
46-47	0.007303144517761243	3.1948697273436735E-4	5.734381561898901E-5	4.095986829927786E-6	1.228796048978336E-4
48-49	0.009285602143446293	3.1948697273436735E-4	5.734381561898901E-5	4.095986829927786E-6	1.269755917277614E-4
50-51	0.011847641905566121	3.215349661493313E-4	5.734381561898901E-5	4.095986829927786E-6	1.3926355221754473E-4
52-53	0.015103951435358712	3.317749332241507E-4	5.734381561898901E-5	4.095986829927786E-6	1.3926355221754473E-4
54-55	0.018876355305722202	3.338229266391146E-4	5.734381561898901E-5	4.095986829927786E-6	1.3926355221754473E-4
56-57	0.02316690151007156	3.932147356730675E-4	7.167976952373627E-5	4.095986829927786E-6	1.3926355221754473E-4
58-59	0.028518308303372215	3.9526272908803144E-4	1.1059164440805024E-4	4.095986829927786E-6	1.3926355221754473E-4
60-61	0.03615732374118753	3.993587159179592E-4	1.1468763123797802E-4	4.095986829927786E-6	1.3926355221754473E-4
62-63	0.04612081170498687	4.0140670933292306E-4	1.1468763123797802E-4	4.095986829927786E-6	1.3926355221754473E-4
64-65	0.058572611667967345	4.0550269616285086E-4	1.1468763123797802E-4	4.095986829927786E-6	1.3926355221754473E-4
66-67	0.07422747333195134	4.0550269616285086E-4	1.1468763123797802E-4	4.095986829927786E-6	1.3926355221754473E-4
68-69	0.09326357212404074	4.0550269616285086E-4	1.1468763123797802E-4	4.095986829927786E-6	1.3926355221754473E-4
70-71	0.11820813191830096	4.0550269616285086E-4	1.1468763123797802E-4	4.095986829927786E-6	1.4131154563250863E-4
72-73	0.15066882754547867	4.0550269616285086E-4	1.1468763123797802E-4	4.095986829927786E-6	1.474555258774003E-4
74-75	0.19408423994929824	4.3212661055738147E-4	1.187836180679058E-4	4.095986829927786E-6	1.474555258774003E-4
76-77	0.24952751767920078	7.536615767067126E-4	1.187836180679058E-4	4.095986829927786E-6	1.474555258774003E-4
78-79	0.3181967368829401	8.601572342848351E-4	1.187836180679058E-4	4.095986829927786E-6	1.515515127073281E-4
80-81	0.4039585091279681	8.970211157541852E-4	1.228796048978336E-4	4.095986829927786E-6	1.515515127073281E-4
82-83	0.5129772945933261	9.011171025841131E-4	1.228796048978336E-4	4.095986829927786E-6	1.5564749953725588E-4
84-85	0.6547353027902968	9.175010499038242E-4	1.228796048978336E-4	4.095986829927786E-6	1.5974348636718367E-4
86-87	0.836062591757785	9.379809840534631E-4	1.269755917277614E-4	8.191973659855573E-6	1.5974348636718367E-4
88-89	1.0569100096538313	9.912288128425244E-4	1.269755917277614E-4	8.191973659855573E-6	1.6179147978214757E-4
90	1.2494193426670224	0.00103628466797173	1.3107157855768916E-4	8.191973659855573E-6	1.6383947319711147E-4
>>END_MODULE
