##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654123_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	32139809
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66424831585029	32.0	32.0	32.0	32.0	32.0
2	31.621454190969214	32.0	32.0	32.0	32.0	32.0
3	35.727692034510845	37.0	37.0	37.0	32.0	37.0
4	36.34658774730117	37.0	37.0	37.0	37.0	37.0
5	36.51933345341287	37.0	37.0	37.0	37.0	37.0
6	40.10785004976228	41.0	41.0	41.0	37.0	41.0
7	40.153726644735194	41.0	41.0	41.0	37.0	41.0
8	40.23883355996297	41.0	41.0	41.0	37.0	41.0
9	40.24827375296474	41.0	41.0	41.0	41.0	41.0
10-11	40.3015579681883	41.0	41.0	41.0	41.0	41.0
12-13	40.26692415938128	41.0	41.0	41.0	41.0	41.0
14-15	40.213794565487305	41.0	41.0	41.0	41.0	41.0
16-17	40.24048927919889	41.0	41.0	41.0	41.0	41.0
18-19	40.237451286658235	41.0	41.0	41.0	41.0	41.0
20-21	40.216687613171565	41.0	41.0	41.0	41.0	41.0
22-23	40.20825644919047	41.0	41.0	41.0	41.0	41.0
24-25	40.1895038953094	41.0	41.0	41.0	41.0	41.0
26-27	40.13997335827354	41.0	41.0	41.0	41.0	41.0
28-29	40.121450566181025	41.0	41.0	41.0	39.0	41.0
30-31	40.098931732917265	41.0	41.0	41.0	37.0	41.0
32-33	40.086095704551326	41.0	41.0	41.0	37.0	41.0
34-35	40.081062398348415	41.0	41.0	41.0	37.0	41.0
36-37	39.9776803589592	41.0	41.0	41.0	37.0	41.0
38-39	40.01670131269293	41.0	41.0	41.0	37.0	41.0
40-41	40.02096126333545	41.0	41.0	41.0	37.0	41.0
42-43	40.00872316945007	41.0	41.0	41.0	37.0	41.0
44-45	39.98977157891635	41.0	41.0	41.0	37.0	41.0
46-47	39.9703911277133	41.0	41.0	41.0	37.0	41.0
48-49	39.94547417814462	41.0	41.0	41.0	37.0	41.0
50-51	39.8839343755901	41.0	41.0	41.0	37.0	41.0
52-53	39.87950264421298	41.0	41.0	41.0	37.0	41.0
54-55	39.838394139181105	41.0	41.0	41.0	37.0	41.0
56-57	39.82686879066394	41.0	41.0	41.0	37.0	41.0
58-59	39.78972885308684	41.0	41.0	41.0	37.0	41.0
60-61	39.79092419310893	41.0	41.0	41.0	37.0	41.0
62-63	39.73935815860014	41.0	41.0	41.0	37.0	41.0
64-65	39.744507660266436	41.0	41.0	41.0	37.0	41.0
66-67	39.68411588880319	41.0	41.0	41.0	37.0	41.0
68-69	39.64769663068004	41.0	41.0	41.0	37.0	41.0
70-71	39.647363539092595	41.0	41.0	41.0	37.0	41.0
72-73	39.62522166824327	41.0	41.0	41.0	37.0	41.0
74-75	39.578940217099614	41.0	41.0	41.0	37.0	41.0
76-77	38.98705300333303	41.0	39.0	41.0	34.5	41.0
78-79	39.36685994306936	41.0	41.0	41.0	37.0	41.0
80-81	39.66363563330448	41.0	41.0	41.0	37.0	41.0
82-83	39.688121715346846	41.0	41.0	41.0	37.0	41.0
84-85	39.70224006309434	41.0	41.0	41.0	37.0	41.0
86-87	39.68444691130554	41.0	41.0	41.0	37.0	41.0
88-89	39.638781129657616	41.0	41.0	41.0	37.0	41.0
90-91	39.600932833795	41.0	41.0	41.0	37.0	41.0
92-93	39.552467906701	41.0	41.0	41.0	37.0	41.0
94-95	39.52612293059987	41.0	41.0	41.0	37.0	41.0
96-97	39.50401240716769	41.0	41.0	41.0	37.0	41.0
98-99	39.45451701346452	41.0	41.0	41.0	37.0	41.0
100-101	38.9103047563226	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	1.0
15	3.0
16	12.0
17	19.0
18	60.0
19	182.0
20	571.0
21	1565.0
22	3935.0
23	8587.0
24	16274.0
25	28567.0
26	48158.0
27	75546.0
28	109693.0
29	149790.0
30	197334.0
31	248604.0
32	307909.0
33	372133.0
34	446575.0
35	541499.0
36	676721.0
37	896331.0
38	1348733.0
39	3043966.0
40	2.3617037E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.452646993065077	12.363282096122925	8.79704863609497	51.38702227471703
2	23.38070521825441	15.836951613495899	35.46123438381355	25.32110878443615
3	20.317661160764736	19.51135638945324	22.85615260769067	37.31482984209135
4	23.18301891588715	27.145024415048642	20.84341571538275	28.828540953681458
5	23.778470494333057	30.859729129068565	24.48570245081419	20.87609792578419
6	19.142444188140633	32.36847798317656	25.970639713509186	22.518438115173613
7	16.267206192793495	22.05017148670672	41.34332285546563	20.33929946503416
8	19.509510940333445	22.907032757247045	30.845083718440797	26.738372583978716
9	18.929720459757554	21.39260068409243	34.03427506367571	25.643403792474313
10-11	22.499036942005475	29.396378802375583	23.385213645793602	24.71937060982534
12-13	22.8609650418271	22.925142461176417	26.322315730003247	27.891576766993232
14-15	21.32127486867369	26.097208042508317	27.31766343400304	25.263853654814948
16-17	22.2468854746461	26.0546679042181	26.307726346475803	25.39072027466
18-19	21.97879582918492	26.423014834966818	26.108890379529015	25.48929895631925
20-21	22.16032615501853	26.209489919495166	26.438879895023643	25.191304030462657
22-23	21.92234247565068	26.51250043209653	26.44566431617562	25.119492776077166
24-25	21.87986616775627	26.134431690411958	26.45873371508622	25.52696842674555
26-27	21.823763793991432	26.275167036618047	26.573703347148083	25.32736582224244
28-29	21.840220529160526	26.392690986598794	26.429664061360803	25.337424422879877
30-31	22.045401701049315	26.157213628743097	26.239743677381533	25.557640992826062
32-33	22.15708977099505	26.274899126262657	26.344112359727106	25.223898743015184
34-35	22.2978368849311	26.48734239295669	26.043977551995646	25.170843170116562
36-37	22.003890875642725	26.182005313099406	26.17085247768585	25.64325133357202
38-39	22.08926941662908	26.354008513242878	26.380198774672248	25.176523295455798
40-41	22.268632648065832	26.620094413131078	25.983494488097303	25.12777845070579
42-43	22.20777976620832	26.167529495897128	26.212774008706774	25.411916729187784
44-45	22.231490236920823	26.337636293980466	26.398951219654105	25.031922249444605
46-47	22.247616655095868	26.25180037628724	26.217266879215117	25.283316089401776
48-49	22.154234021739207	26.074209090663857	26.08100129033125	25.690555597265686
50-51	22.295908790248255	26.129991936168633	26.331121009462127	25.242978264120985
52-53	22.645112794540875	26.515417375380174	25.77989184689928	25.059577983179675
54-55	22.363925684810386	26.22542031908155	25.951135863937463	25.459518132170604
56-57	22.31186096967782	26.07433510261371	26.42881294036315	25.18499098734532
58-59	22.334645797054986	26.136658123886175	26.274261617422805	25.254434461636034
60-61	22.37110836595202	25.936638266892004	26.275697531369897	25.41655583578608
62-63	22.431328076654093	26.031823960123724	26.195552375560165	25.341295587662017
64-65	22.418652207920715	26.3300320173029	25.967893897564853	25.28342187721153
66-67	22.433174696215524	26.081051072829958	26.006252868522022	25.47952136243249
68-69	22.42657851513679	26.266111288962545	26.32767046002047	24.979639735880195
70-71	22.757433001546463	26.38162691010392	26.035997911499724	24.8249421768499
72-73	22.640390706892	25.870384711365553	25.98938515369878	25.499839428043668
74-75	22.56338704837901	25.979653872487855	26.267964601147625	25.188994477985503
76-77	22.581232514480718	26.242828636598308	26.102143295251068	25.073795553669903
78-79	22.451430210720076	26.081717036950376	25.933720750781287	25.53313200154826
80-81	22.61285062397228	26.183761701881924	26.141530585947166	25.061857088198625
82-83	22.825221207755156	26.24112203031449	25.7447578484365	25.18889891349386
84-85	22.658392276071087	26.128887386978562	25.744165125561263	25.468555211389095
86-87	22.70349949645731	26.026806573550914	26.158679467658285	25.111014462333493
88-89	22.89140718965727	26.10805355672245	25.952928104531846	25.047611149088432
90-91	22.920434903642395	25.88207198119939	25.9831506777156	25.21434243744261
92-93	22.854244216470562	25.87928376608297	26.19430386327089	25.07216815417557
94-95	23.013011195039795	26.002381879668096	25.975848962344354	25.008757962947758
96-97	22.95772821798661	26.028675217080476	25.72436569240346	25.289230872529455
98-99	23.026457624561488	26.249689287201427	25.8895502459271	24.83430284230998
100-101	23.082158017802783	26.39892943981092	25.57679480920375	24.942117733182545
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	24.0
2	22.5
3	21.0
4	32.5
5	51.5
6	62.5
7	75.5
8	107.0
9	132.5
10	131.0
11	159.5
12	204.5
13	235.0
14	280.5
15	332.5
16	389.5
17	465.0
18	597.0
19	741.5
20	969.5
21	1361.0
22	2060.0
23	3141.0
24	4686.5
25	7084.0
26	10538.0
27	15598.0
28	22499.0
29	32605.0
30	45918.0
31	62632.0
32	87388.0
33	122109.5
34	161745.5
35	215845.5
36	295549.5
37	396539.0
38	528052.5
39	716232.0
40	932788.0
41	1147694.5
42	1366403.5
43	1572760.0
44	1765563.5
45	1914945.0
46	1994612.0
47	2002933.0
48	1939857.5
49	1841489.0
50	1719963.0
51	1560984.0
52	1414085.5
53	1262352.0
54	1098037.5
55	962603.0
56	821911.5
57	701217.5
58	590006.0
59	462050.5
60	377490.0
61	350498.0
62	315734.0
63	269764.5
64	224160.0
65	179296.0
66	150879.5
67	123307.5
68	92541.0
69	69721.5
70	52511.5
71	36522.5
72	27522.0
73	22028.0
74	17226.0
75	11720.0
76	6773.5
77	2415.5
78	507.0
79	176.0
80	69.0
81	28.0
82	9.5
83	5.5
84	2.5
85	0.5
86	2.0
87	2.0
88	1.5
89	3.5
90	3.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024745013263768927
2	0.0
3	0.0022433238480041996
4	0.0
5	0.0
6	0.0
7	0.0
8	9.178648199185005E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0024300082181571148
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.289390487665934E-4
26-27	0.0
28-29	6.362825616045198E-4
30-31	0.0
32-33	4.667109253822883E-6
34-35	7.934085731498902E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	3.88925771151907E-5
74-75	5.584974073741385E-4
76-77	0.0
78-79	0.0015214776167462602
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.001642822457345655
88-89	4.822679562283647E-5
90-91	0.0
92-93	3.531446002059315E-4
94-95	0.0015121433982386143
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.2139809E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.381818937133772
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.15751926785041	16.39828418905833
2	15.026378224512927	7.928463791999793
3	6.70520493157381	5.306865073234702
4	3.8815311135559174	4.096074041467338
5	2.4161230916528935	3.187086096690725
6	1.629328520428215	2.5790790009027145
7	1.2647591179524662	2.3356652234237685
8	0.9521309071501564	2.009515615750748
9	0.703723036925194	1.6708944367845333
>10	4.5667865762055015	22.741970472807797
>50	0.3973765878569918	7.265259168223512
>100	0.2608601077153286	13.422396338305367
>500	0.025101336013151142	4.52990934796242
>1k	0.012807135839551663	5.8661416790597425
>5k	3.453751163873934E-4	0.5826330205368357
>10k+	2.4669651170528103E-5	0.07976250379170578
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.289390487665935E-5	3.111406169215256E-6	0.0	0.0	3.111406169215256E-6
2	7.156234189195088E-5	3.111406169215256E-6	0.0	0.0	3.111406169215256E-6
3	1.3690187144547124E-4	3.111406169215256E-6	0.0	0.0	3.111406169215256E-6
4	1.9290718249134585E-4	3.111406169215256E-6	6.222812338430512E-6	3.111406169215256E-6	3.111406169215256E-6
5	2.2713265035271367E-4	3.111406169215256E-6	6.222812338430512E-6	3.111406169215256E-6	3.111406169215256E-6
6	3.235862415983866E-4	3.111406169215256E-6	9.334218507645767E-6	3.111406169215256E-6	3.111406169215256E-6
7	3.5781170945975443E-4	3.111406169215256E-6	9.334218507645767E-6	3.111406169215256E-6	3.111406169215256E-6
8	4.7293373772071886E-4	3.111406169215256E-6	1.2445624676861023E-5	3.111406169215256E-6	3.111406169215256E-6
9	5.102706117513019E-4	3.111406169215256E-6	1.2445624676861023E-5	3.111406169215256E-6	9.334218507645767E-6
10-11	5.802772505586451E-4	3.111406169215256E-6	1.2445624676861023E-5	3.111406169215256E-6	1.2445624676861023E-5
12-13	6.456167801121655E-4	6.222812338430512E-6	1.2445624676861023E-5	1.0889921592253395E-5	1.711273393068391E-5
14-15	7.451817775270537E-4	6.222812338430512E-6	1.2445624676861023E-5	1.2445624676861023E-5	2.177984318450679E-5
16-17	8.369682595189038E-4	9.334218507645767E-6	1.2445624676861023E-5	1.2445624676861023E-5	2.4891249353722047E-5
18-19	9.147534137492852E-4	1.2445624676861023E-5	1.2445624676861023E-5	1.2445624676861023E-5	2.80026555229373E-5
20-21	0.001028319738925642	1.2445624676861023E-5	1.555703084607628E-5	1.2445624676861023E-5	3.111406169215256E-5
22-23	0.0011434417671866066	1.2445624676861023E-5	1.555703084607628E-5	1.555703084607628E-5	3.422546786136781E-5
24-25	0.0013114577003242303	2.6446952438329672E-5	1.555703084607628E-5	1.555703084607628E-5	3.5781170945975435E-5
26-27	0.0014701394149542082	4.0448280199798324E-5	1.555703084607628E-5	1.555703084607628E-5	3.88925771151907E-5
28-29	0.0016739365190378075	4.0448280199798324E-5	1.555703084607628E-5	1.555703084607628E-5	4.0448280199798324E-5
30-31	0.002014635494566878	4.0448280199798324E-5	1.711273393068391E-5	1.555703084607628E-5	4.822679562283646E-5
32-33	0.0025015705600490656	4.0448280199798324E-5	1.8668437015291533E-5	1.555703084607628E-5	5.60053110458746E-5
34-35	0.0030538451550847735	4.355968636901358E-5	1.8668437015291533E-5	1.555703084607628E-5	5.911671721508986E-5
36-37	0.0038441423220654486	4.511538945362121E-5	1.8668437015291533E-5	1.555703084607628E-5	6.6895232638128E-5
38-39	0.005199159708758692	4.9782498707444094E-5	1.8668437015291533E-5	1.555703084607628E-5	7.934085731498903E-5
40-41	0.007061336301034023	5.4449607961266975E-5	1.8668437015291533E-5	1.555703084607628E-5	8.711937273802716E-5
42-43	0.009460230457498985	6.378382646891274E-5	1.8668437015291533E-5	1.555703084607628E-5	9.956499741488819E-5
44-45	0.0122776087437234	6.845093572273563E-5	1.8668437015291533E-5	1.555703084607628E-5	1.0423210666871107E-4
46-47	0.01524744593223936	7.467374806116613E-5	1.8668437015291533E-5	1.555703084607628E-5	1.1045491900714158E-4
48-49	0.01923626864117332	7.622945114577377E-5	1.8668437015291533E-5	1.555703084607628E-5	1.1823343443017971E-4
50-51	0.025077933723874964	8.400796656881191E-5	2.0224140099899162E-5	1.555703084607628E-5	1.2134484059939498E-4
52-53	0.03245352204799973	8.400796656881191E-5	2.177984318450679E-5	1.555703084607628E-5	1.2601194985321787E-4
54-55	0.04078431206607357	8.400796656881191E-5	2.333554626911442E-5	1.555703084607628E-5	1.3067905910704074E-4
56-57	0.049514917776891584	9.334218507645767E-5	3.733687403058307E-5	1.555703084607628E-5	1.353461683608636E-4
58-59	0.05921783791558936	9.334218507645767E-5	4.355968636901358E-5	1.555703084607628E-5	1.446803868685094E-4
60-61	0.07116252619920672	9.334218507645767E-5	4.355968636901358E-5	1.555703084607628E-5	1.4779179303772466E-4
62-63	0.08575190972665706	9.334218507645767E-5	4.355968636901358E-5	1.555703084607628E-5	1.4934749612233227E-4
64-65	0.10353981879606067	1.0267640358410344E-4	4.355968636901358E-5	1.555703084607628E-5	1.4934749612233227E-4
66-67	0.12441735419149505	1.0267640358410344E-4	4.355968636901358E-5	1.555703084607628E-5	1.5245890229154753E-4
68-69	0.14858986872012836	1.0267640358410344E-4	4.511538945362121E-5	1.555703084607628E-5	1.6023741771458566E-4
70-71	0.17674809455152643	1.0267640358410344E-4	4.667109253822884E-5	1.555703084607628E-5	1.6490452696840856E-4
72-73	0.21050840719059655	1.057878097533187E-4	4.667109253822884E-5	1.555703084607628E-5	1.6490452696840856E-4
74-75	0.25286709077829306	1.229005436840026E-4	4.667109253822884E-5	1.555703084607628E-5	1.6801593313762382E-4
76-77	0.3055649770662918	1.400132776146865E-4	4.667109253822884E-5	1.555703084607628E-5	1.6801593313762382E-4
78-79	0.37022155296566944	1.400132776146865E-4	4.667109253822884E-5	1.555703084607628E-5	1.6801593313762382E-4
80-81	0.44657701606129646	1.400132776146865E-4	4.667109253822884E-5	1.555703084607628E-5	1.7112733930683906E-4
82-83	0.5344011222966509	1.400132776146865E-4	4.822679562283646E-5	1.555703084607628E-5	1.7423874547605432E-4
84-85	0.6405498551655986	1.46236089953117E-4	4.9782498707444094E-5	1.555703084607628E-5	1.8046155781448482E-4
86-87	0.7744072156744927	1.509031992069399E-4	4.9782498707444094E-5	1.555703084607628E-5	1.8824007323752298E-4
88-89	0.9371897636354964	1.6490452696840856E-4	4.9782498707444094E-5	1.555703084607628E-5	1.897957763221306E-4
90	1.0789080918309129	1.6801593313762382E-4	5.289390487665935E-5	1.555703084607628E-5	1.897957763221306E-4
>>END_MODULE
