##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654122_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33557947
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.37552684614467	32.0	32.0	32.0	32.0	32.0
2	31.387622848322636	32.0	32.0	32.0	32.0	32.0
3	35.314045105321846	37.0	37.0	37.0	32.0	37.0
4	35.937838628805274	37.0	37.0	37.0	32.0	37.0
5	36.108840657028274	37.0	37.0	37.0	37.0	37.0
6	39.344402236525376	41.0	41.0	41.0	37.0	41.0
7	39.41996633465092	41.0	41.0	41.0	37.0	41.0
8	39.572898961906105	41.0	41.0	41.0	37.0	41.0
9	39.67567738872703	41.0	41.0	41.0	37.0	41.0
10-11	39.73091269558296	41.0	41.0	41.0	37.0	41.0
12-13	39.66678532211759	41.0	41.0	41.0	37.0	41.0
14-15	39.56186755107516	41.0	41.0	41.0	37.0	41.0
16-17	39.62298916855671	41.0	41.0	41.0	37.0	41.0
18-19	39.583767535004455	41.0	41.0	41.0	37.0	41.0
20-21	39.57105658161984	41.0	41.0	41.0	37.0	41.0
22-23	39.43926666610446	41.0	41.0	41.0	37.0	41.0
24-25	39.46858970544295	41.0	41.0	41.0	37.0	41.0
26-27	39.42313221664007	41.0	41.0	41.0	37.0	41.0
28-29	39.48671186589573	41.0	41.0	41.0	37.0	41.0
30-31	39.43474901787049	41.0	41.0	41.0	37.0	41.0
32-33	39.43486015398975	41.0	41.0	41.0	37.0	41.0
34-35	39.3421592208844	41.0	41.0	41.0	37.0	41.0
36-37	39.27114839593733	41.0	41.0	41.0	37.0	41.0
38-39	39.222087170588836	41.0	41.0	41.0	37.0	41.0
40-41	39.151418768257784	41.0	41.0	41.0	37.0	41.0
42-43	39.12385505883301	41.0	41.0	41.0	37.0	41.0
44-45	39.12161208789084	41.0	41.0	41.0	37.0	41.0
46-47	39.065253589559575	41.0	41.0	41.0	37.0	41.0
48-49	39.01644319898354	41.0	41.0	41.0	37.0	41.0
50-51	39.064600495375956	41.0	41.0	41.0	37.0	41.0
52-53	39.05782205627776	41.0	41.0	41.0	37.0	41.0
54-55	39.0427174075935	41.0	41.0	41.0	37.0	41.0
56-57	38.99632534135655	41.0	41.0	41.0	37.0	41.0
58-59	38.901151238483095	41.0	41.0	41.0	32.0	41.0
60-61	38.94013333115998	41.0	41.0	41.0	37.0	41.0
62-63	38.927335751498745	41.0	41.0	41.0	37.0	41.0
64-65	38.896028130087934	41.0	41.0	41.0	34.5	41.0
66-67	38.85140652376619	41.0	41.0	41.0	32.0	41.0
68-69	38.82479163579345	41.0	41.0	41.0	32.0	41.0
70-71	38.79686236467326	41.0	41.0	41.0	32.0	41.0
72-73	38.7635298428715	41.0	41.0	41.0	32.0	41.0
74-75	38.74161944412154	41.0	41.0	41.0	32.0	41.0
76-77	37.88911324044942	41.0	39.0	41.0	29.5	41.0
78-79	38.33938485569454	41.0	39.0	41.0	32.0	41.0
80-81	38.672744983475894	41.0	41.0	41.0	32.0	41.0
82-83	38.834032263654265	41.0	41.0	41.0	32.0	41.0
84-85	38.7906481436424	41.0	41.0	41.0	32.0	41.0
86-87	38.7242331302329	41.0	41.0	41.0	32.0	41.0
88-89	38.667209826632124	41.0	41.0	41.0	32.0	41.0
90-91	38.613809316165856	41.0	41.0	41.0	32.0	41.0
92-93	38.54395066241686	41.0	41.0	41.0	32.0	41.0
94-95	38.53378588684224	41.0	41.0	41.0	32.0	41.0
96-97	38.43403540151011	41.0	37.0	41.0	32.0	41.0
98-99	38.225982849904376	41.0	37.0	41.0	32.0	41.0
100-101	37.24328629221567	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	38.0
13	155.0
14	464.0
15	1877.0
16	7610.0
17	20447.0
18	35525.0
19	49209.0
20	60107.0
21	72072.0
22	86005.0
23	100816.0
24	118400.0
25	137463.0
26	158652.0
27	183035.0
28	210182.0
29	245441.0
30	286038.0
31	334718.0
32	394106.0
33	469812.0
34	571197.0
35	712721.0
36	926517.0
37	1292526.0
38	2122448.0
39	5255542.0
40	1.9704824E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.442672601979524	18.842175760008573	12.63956315111571	38.07558848689619
2	28.773509297216542	20.27758730294198	34.322895259355406	16.626008140486068
3	21.59166771932582	23.404232050358615	29.968018531330546	25.03608169898502
4	24.75361201327364	30.006248594408945	23.396562370159295	21.84357702215812
5	27.488351104296715	32.106821756398844	22.087203881185076	18.317623258119358
6	21.231242185345845	35.47068299499966	22.60542636890153	20.692648450752962
7	21.317919120618434	18.947410579079822	37.943364056209994	21.79130624409175
8	22.200321134066993	23.220228579537363	27.655490963139073	26.923959323256575
9	22.934412525295425	22.52886030244937	29.714034651762216	24.822692520492986
10-11	25.829057238306742	28.410428896036212	22.778299496666595	22.982214368990455
12-13	26.575086677304625	22.791384084608158	25.263448406814703	25.370080831272514
14-15	24.52683562555242	26.471947166493827	26.328924412449904	22.67229279550385
16-17	25.898888207953142	25.954295958682	25.315857480126063	22.8309583532388
18-19	25.544011027018897	26.755439804828285	25.24753719585827	22.453011972294544
20-21	25.751564699887197	26.758684619090882	25.16063374079696	22.329116940224957
22-23	25.479208692387722	26.623521633793757	25.372381139634154	22.52488853418437
24-25	24.850142375192227	26.638055988099868	25.936662957100566	22.57513867960734
26-27	25.20026776369842	26.528522439111068	25.780687656488638	22.490522140701874
28-29	25.581701975610503	26.36939739587602	25.579672604652774	22.469228023860694
30-31	25.164237550050366	26.602041537284745	25.71758188902319	22.516139023641703
32-33	25.244102555712537	26.67963023913531	25.57282288547798	22.50344431967417
34-35	25.52795914481896	26.35966675792175	25.48524199051867	22.627132106740618
36-37	25.319473506528873	26.357661271710096	25.762030376888074	22.56083484487296
38-39	25.363674800068107	26.705469691164136	25.44119174741094	22.48966376135681
40-41	25.5814710595973	26.37552887249032	25.349874651151932	22.69312541676045
42-43	25.363227810378547	26.389333356060384	25.718872404061322	22.528566429499747
44-45	25.49676659526419	26.408500282543528	25.751712850308607	22.343020271883667
46-47	25.50279142028954	26.41367601424301	25.610699131021242	22.472833434446216
48-49	25.06218468393332	26.550939827921326	25.816399103276456	22.5704763848689
50-51	25.54417586987666	26.44832236012531	25.56096920946922	22.446532560528805
52-53	25.811668034518775	26.260611892316472	25.539559807256744	22.388160265908006
54-55	25.356736819871823	26.416594989324686	25.73967321807108	22.486994972732415
56-57	25.08475484925819	26.643329506387115	25.941629325481806	22.33028631887289
58-59	25.556104489943916	26.134909266052535	25.80207156295944	22.506914681044105
60-61	25.483580944461742	26.163636319750243	25.75874691194442	22.5940358238436
62-63	25.412281451594986	26.458080177663927	25.709308828085554	22.42032954265553
64-65	25.582253288617444	26.339349662838433	25.606249095035523	22.472147953508596
66-67	25.36051608575124	26.52820809069928	25.870696684742455	22.240579138807032
68-69	25.591400403232562	26.68132416046508	25.72216729544238	22.00510814085998
70-71	25.835345756603257	26.436301331469913	25.324101789514803	22.404251122412024
72-73	25.253614292912495	26.667103622280585	25.780657857287874	22.298624227519042
74-75	25.222107026506407	26.56542552044693	25.842912322385708	22.369555130660952
76-77	25.66508140223443	26.39103951552887	25.680961959464675	22.262917122772024
78-79	25.410590522715825	26.552104036638475	25.86374696878805	22.17355847185765
80-81	25.383109684187705	26.660589467734162	25.84631543776603	22.1099854103121
82-83	25.78829078165486	26.351190131544033	25.729877625898872	22.130641460902236
84-85	25.5977152316524	26.378791914208694	25.780972009948737	22.242520844190167
86-87	25.420445118409656	26.690223332196094	25.803542153517316	22.08578939587693
88-89	25.66727972682321	26.575355434659315	25.616051405568683	22.14131343294879
90-91	25.378236243362934	26.61145298924734	25.84813115255116	22.162179614838568
92-93	25.577226875203063	26.629543475396893	25.737550166773353	22.055679482626687
94-95	25.930874138397087	26.418456111156026	25.600579797089495	22.050089953357396
96-97	25.52096919456119	26.4985777617984	25.882325945959195	22.098127097681218
98-99	25.639357649467094	26.801012720119672	25.701794439759844	21.857835190653397
100-101	26.063829406815188	26.521985934885283	25.641599128138825	21.772585530160708
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	18.5
2	18.0
3	18.0
4	27.0
5	46.0
6	60.0
7	72.5
8	93.0
9	105.5
10	133.0
11	189.5
12	223.0
13	267.0
14	358.5
15	460.0
16	590.5
17	746.5
18	935.0
19	1197.0
20	1535.5
21	1993.5
22	2613.5
23	3436.0
24	4632.0
25	6405.5
26	8784.5
27	12056.0
28	17060.0
29	23090.5
30	31258.5
31	43448.0
32	61501.0
33	84689.0
34	118775.0
35	176526.0
36	262812.5
37	371516.0
38	519993.5
39	719386.0
40	953693.0
41	1207772.5
42	1476842.0
43	1716736.5
44	1909258.5
45	2049353.5
46	2123697.0
47	2155794.0
48	2109648.0
49	1984056.5
50	1818546.5
51	1659844.5
52	1518683.5
53	1362558.5
54	1188037.0
55	1018588.5
56	855388.5
57	703937.5
58	565155.0
59	462072.0
60	396889.0
61	331560.5
62	269181.0
63	216979.0
64	186774.0
65	163074.5
66	137425.0
67	117399.5
68	95076.0
69	71688.0
70	63060.5
71	56664.5
72	42585.0
73	31870.0
74	22266.5
75	14408.5
76	9721.0
77	4957.5
78	2644.0
79	1613.0
80	999.5
81	663.0
82	469.5
83	351.0
84	286.5
85	236.0
86	205.5
87	178.0
88	152.0
89	138.5
90	122.5
91	107.5
92	87.5
93	64.5
94	59.0
95	62.5
96	52.0
97	56.5
98	68.0
99	106.5
100	487.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04196323452087221
2	0.0
3	2.264739258334248E-4
4	0.0
5	0.008448073417602096
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.1157432544964685E-4
12-13	4.2612857097604933E-4
14-15	0.0
16-17	0.001095120628207679
18-19	3.128916080593369E-5
20-21	0.0014109921563437715
22-23	3.7249000959444867E-4
24-25	0.003017169077715034
26-27	0.0
28-29	0.00223643001760507
30-31	0.0
32-33	0.004799161283614877
34-35	0.0
36-37	0.0
38-39	0.002291558539025048
40-41	0.0
42-43	8.582169821056098E-4
44-45	8.522571419520987E-4
46-47	0.001691104643558797
48-49	0.0013335142343481262
50-51	0.0
52-53	0.0032138438027809035
54-55	4.618876118971164E-4
56-57	0.0034716068894202617
58-59	0.0
60-61	5.363856138160061E-5
62-63	0.003300261485006815
64-65	0.0
66-67	0.002415225222210405
68-69	4.201687308225381E-4
70-71	0.0029024421547599443
72-73	0.0
74-75	2.4584340633233614E-4
76-77	0.003546104891339151
78-79	0.0
80-81	0.004079510585078402
82-83	0.001305204993618948
84-85	0.002784735311728098
86-87	0.0
88-89	0.0010057230259050115
90-91	5.363856138160061E-5
92-93	3.8142976982471545E-4
94-95	0.0
96-97	0.0023973457017498717
98-99	2.3839360614044715E-5
100-101	9.267551438709883E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.3557947E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.85052416397721
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.313991661688874	18.601053138511183
2	15.291337611519037	9.129088857443653
3	6.995146684412489	6.264263854008603
4	3.739700038617265	4.465280254750848
5	2.4422978748363215	3.6451935864215903
6	1.6561934524994362	2.9662945604433157
7	1.2174476103388485	2.543901451755728
8	0.8960886393683892	2.139897246602525
9	0.7116562445533965	1.9118980732037791
>10	4.188480771382717	23.356609626912594
>50	0.33333349689539915	6.873714091140934
>100	0.19286057335990062	10.783074227069758
>500	0.01457544058341564	2.957716612792264
>1k	0.006543575800764994	3.4276855085391045
>5k	2.51872104623466E-4	0.522138892280534
>10k+	9.445203923379977E-5	0.41219001812353256
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3839360614044715E-5	2.9799200767555894E-6	8.939760230266768E-6	5.959840153511179E-6	0.0
2	2.9799200767555894E-5	8.939760230266768E-6	8.939760230266768E-6	5.959840153511179E-6	0.0
3	5.66184814583562E-5	1.4899600383777947E-5	1.1919680307022358E-5	5.959840153511179E-6	8.939760230266768E-6
4	1.0727712276320121E-4	1.4899600383777947E-5	7.151808184213415E-5	5.959840153511179E-6	8.939760230266768E-6
5	1.132369629167124E-4	1.4899600383777947E-5	8.34377621491565E-5	8.939760230266768E-6	8.939760230266768E-6
6	1.4601608376102387E-4	1.4899600383777947E-5	1.3111648337724594E-4	8.939760230266768E-6	8.939760230266768E-6
7	1.4899600383777947E-4	1.4899600383777947E-5	1.6091568414480182E-4	1.1919680307022358E-5	8.939760230266768E-6
8	1.698554443750686E-4	1.4899600383777947E-5	1.8177512468209095E-4	1.1919680307022358E-5	1.1919680307022358E-5
9	1.9071488491235772E-4	1.4899600383777947E-5	1.9071488491235772E-4	1.1919680307022358E-5	1.1919680307022358E-5
10-11	2.2200404571829141E-4	1.4899600383777947E-5	2.1455424552640242E-4	1.4899600383777947E-5	1.1919680307022358E-5
12-13	2.532932065242251E-4	1.6389560422155743E-5	2.3243376598693598E-4	1.7879520460533537E-5	1.4899600383777947E-5
14-15	3.3226108855824823E-4	1.7879520460533537E-5	2.696827669463808E-4	1.7879520460533537E-5	1.4899600383777947E-5
16-17	4.365582912446938E-4	1.7879520460533537E-5	4.708273721273831E-4	1.7879520460533537E-5	1.4899600383777947E-5
18-19	5.781044948905843E-4	1.7879520460533537E-5	5.065864130484501E-4	2.3839360614044715E-5	1.4899600383777947E-5
20-21	7.941487004553646E-4	1.7879520460533537E-5	5.646948545451841E-4	2.532932065242251E-5	1.4899600383777947E-5
22-23	0.0010683013475168788	2.3839360614044715E-5	6.421727765408296E-4	2.6819280690800303E-5	1.4899600383777947E-5
24-25	0.0014840001982242834	2.9799200767555894E-5	6.585623369629852E-4	2.9799200767555894E-5	1.4899600383777947E-5
26-27	0.0020129360118484006	2.9799200767555894E-5	6.809117375386522E-4	3.2779120844311485E-5	1.7879520460533537E-5
28-29	0.002643189108082208	2.9799200767555894E-5	7.539197794191641E-4	3.575904092106707E-5	1.7879520460533537E-5
30-31	0.003347940206234905	2.9799200767555894E-5	7.777591400332088E-4	3.575904092106707E-5	1.7879520460533537E-5
32-33	0.0042225467487626706	3.426908088268928E-5	7.98618580570498E-4	3.575904092106707E-5	1.7879520460533537E-5
34-35	0.005180591053439592	4.0228921036200455E-5	9.893334654828556E-4	3.575904092106707E-5	1.936948049891133E-5
36-37	0.0064157679252547836	4.469880115133384E-5	0.0010206226262887894	3.575904092106707E-5	2.0859440537289124E-5
38-39	0.00795191672482229	5.3638561381600606E-5	0.0010355222266725673	3.575904092106707E-5	2.0859440537289124E-5
40-41	0.009839696093446957	6.257832161186738E-5	0.0010861808679774123	3.575904092106707E-5	2.0859440537289124E-5
42-43	0.012344318917960029	7.896788203402312E-5	0.0011740885102417024	3.575904092106707E-5	2.234940057566692E-5
44-45	0.01525570083295024	8.19478021107787E-5	0.0011934579907406134	3.724900095944487E-5	2.3839360614044715E-5
46-47	0.018399516513927384	8.34377621491565E-5	0.0012888154331967923	4.171888107457825E-5	2.532932065242251E-5
48-49	0.02203352904753083	8.49277221875343E-5	0.0013558636349237931	4.320884111295604E-5	2.8309240729178097E-5
50-51	0.026954867054292682	8.939760230266769E-5	0.001372253195345949	4.469880115133384E-5	3.2779120844311485E-5
52-53	0.033245478336323735	9.237752237942327E-5	0.0014512210773799722	4.469880115133384E-5	3.2779120844311485E-5
54-55	0.04029447927788908	9.237752237942327E-5	0.0015912773209874847	4.469880115133384E-5	3.2779120844311485E-5
56-57	0.047699580668626715	9.535744245617886E-5	0.0017536829651706644	4.469880115133384E-5	3.2779120844311485E-5
58-59	0.05580943315751706	9.535744245617886E-5	0.0018013616863987538	4.469880115133384E-5	3.2779120844311485E-5
60-61	0.0658174947353007	9.535744245617886E-5	0.0018698998481641324	4.618876118971164E-5	3.2779120844311485E-5
62-63	0.07836295825844174	1.0131728260969004E-4	0.0019861167311576	4.767872122808943E-5	3.2779120844311485E-5
64-65	0.09362610889158386	1.0131728260969004E-4	0.00206061473307649	5.065864130484502E-5	3.873896099782266E-5
66-67	0.11113313934252295	1.0429720268644562E-4	0.0020665745732300015	5.065864130484502E-5	4.171888107457825E-5
68-69	0.1318033549549381	1.0429720268644562E-4	0.002084454093690535	5.065864130484502E-5	4.171888107457825E-5
70-71	0.15534621352134564	1.0727712276320121E-4	0.002094883813959179	5.065864130484502E-5	4.469880115133384E-5
72-73	0.183262104800392	1.0727712276320121E-4	0.0021574621355710463	5.065864130484502E-5	4.767872122808943E-5
74-75	0.21871272399351485	1.08767082801579E-4	0.002160442055647802	5.065864130484502E-5	4.767872122808943E-5
76-77	0.2625980069638944	1.117470028783346E-4	0.002170871775916447	5.065864130484502E-5	4.9168681266467225E-5
78-79	0.31650327119236465	1.1919680307022358E-4	0.0021813014961850914	5.065864130484502E-5	5.065864130484502E-5
80-81	0.3807443882070617	1.1919680307022358E-4	0.0022021609367223805	5.065864130484502E-5	5.065864130484502E-5
82-83	0.455674478537081	1.1919680307022358E-4	0.0022304701774515587	5.065864130484502E-5	5.065864130484502E-5
84-85	0.5490547440223325	1.2515664322373477E-4	0.0022528195780272256	5.214860134322281E-5	5.065864130484502E-5
86-87	0.6681785390506756	1.2515664322373477E-4	0.0023109280195239596	5.3638561381600606E-5	5.065864130484502E-5
88-89	0.8158916872954117	1.2515664322373477E-4	0.002334767380138004	5.3638561381600606E-5	5.3638561381600606E-5
90	0.9441489373590106	1.3111648337724594E-4	0.0023392372602531377	5.3638561381600606E-5	5.66184814583562E-5
>>END_MODULE
