##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654117_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38275839
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.615696601712635	32.0	32.0	32.0	32.0	32.0
2	31.611458027085963	32.0	32.0	32.0	32.0	32.0
3	35.708644505480336	37.0	37.0	37.0	32.0	37.0
4	36.333410431577995	37.0	37.0	37.0	37.0	37.0
5	36.51993851787285	37.0	37.0	37.0	37.0	37.0
6	40.094427531686506	41.0	41.0	41.0	37.0	41.0
7	40.12736948757674	41.0	41.0	41.0	37.0	41.0
8	40.21925450151465	41.0	41.0	41.0	37.0	41.0
9	40.23814320569171	41.0	41.0	41.0	41.0	41.0
10-11	40.29985209468563	41.0	41.0	41.0	41.0	41.0
12-13	40.27541607905708	41.0	41.0	41.0	41.0	41.0
14-15	40.22463849845329	41.0	41.0	41.0	41.0	41.0
16-17	40.24975554422204	41.0	41.0	41.0	41.0	41.0
18-19	40.24497928053256	41.0	41.0	41.0	41.0	41.0
20-21	40.22617881739966	41.0	41.0	41.0	41.0	41.0
22-23	40.21651074715828	41.0	41.0	41.0	41.0	41.0
24-25	40.199854613245705	41.0	41.0	41.0	41.0	41.0
26-27	40.149927334055306	41.0	41.0	41.0	41.0	41.0
28-29	40.12783897434619	41.0	41.0	41.0	41.0	41.0
30-31	40.10483561183335	41.0	41.0	41.0	37.0	41.0
32-33	40.09917565752119	41.0	41.0	41.0	37.0	41.0
34-35	40.08807818686874	41.0	41.0	41.0	37.0	41.0
36-37	39.983657863123526	41.0	41.0	41.0	37.0	41.0
38-39	40.02384842563477	41.0	41.0	41.0	37.0	41.0
40-41	40.02963483047361	41.0	41.0	41.0	37.0	41.0
42-43	40.01396423994782	41.0	41.0	41.0	37.0	41.0
44-45	39.99735093984485	41.0	41.0	41.0	37.0	41.0
46-47	39.9759147670153	41.0	41.0	41.0	37.0	41.0
48-49	39.95489158317339	41.0	41.0	41.0	37.0	41.0
50-51	39.89133306783948	41.0	41.0	41.0	37.0	41.0
52-53	39.890030993180844	41.0	41.0	41.0	37.0	41.0
54-55	39.85004311727824	41.0	41.0	41.0	37.0	41.0
56-57	39.83521953627195	41.0	41.0	41.0	37.0	41.0
58-59	39.801880319331474	41.0	41.0	41.0	37.0	41.0
60-61	39.798639123233855	41.0	41.0	41.0	37.0	41.0
62-63	39.75124937169895	41.0	41.0	41.0	37.0	41.0
64-65	39.757719144967666	41.0	41.0	41.0	37.0	41.0
66-67	39.700060644000516	41.0	41.0	41.0	37.0	41.0
68-69	39.667337141845536	41.0	41.0	41.0	37.0	41.0
70-71	39.666606210774376	41.0	41.0	41.0	37.0	41.0
72-73	39.63919444064962	41.0	41.0	41.0	37.0	41.0
74-75	39.5955339737948	41.0	41.0	41.0	37.0	41.0
76-77	39.03588877568431	41.0	39.0	41.0	34.5	41.0
78-79	39.39460835855225	41.0	41.0	41.0	37.0	41.0
80-81	39.67473286477143	41.0	41.0	41.0	37.0	41.0
82-83	39.69760549207034	41.0	41.0	41.0	37.0	41.0
84-85	39.711729597880264	41.0	41.0	41.0	37.0	41.0
86-87	39.69157513438177	41.0	41.0	41.0	37.0	41.0
88-89	39.647882767507724	41.0	41.0	41.0	37.0	41.0
90-91	39.614967969741954	41.0	41.0	41.0	37.0	41.0
92-93	39.565817799578475	41.0	41.0	41.0	37.0	41.0
94-95	39.54204922849634	41.0	41.0	41.0	37.0	41.0
96-97	39.520066575157244	41.0	41.0	41.0	37.0	41.0
98-99	39.473905980219016	41.0	41.0	41.0	37.0	41.0
100-101	38.944150917240506	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	8.0
17	20.0
18	65.0
19	206.0
20	660.0
21	1938.0
22	5037.0
23	10667.0
24	20300.0
25	34351.0
26	56419.0
27	87969.0
28	127580.0
29	175680.0
30	232102.0
31	294088.0
32	363237.0
33	444351.0
34	531836.0
35	644444.0
36	803710.0
37	1064444.0
38	1596365.0
39	3566854.0
40	2.8213506E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.68797066900111	10.994020891795726	6.144420293004238	45.17358814619893
2	24.301408520398468	12.330770855212345	30.287900416761605	33.07992020762758
3	20.791088228025504	13.474955301701657	20.364916507918352	45.369039962354485
4	23.91572657623521	20.128820690253193	20.439497093714916	35.51595563979669
5	25.358553211596487	25.120073788585014	23.700057887692548	25.82131511212595
6	21.00054553996844	29.814439861135376	25.230433224468314	23.954581374427875
7	16.21560013354639	25.74041290120381	39.46307747819714	18.580909487052654
8	18.305083992969397	25.160378597027734	32.45284290679227	24.081694503210603
9	18.47729059577244	22.82598168520878	34.92807825845438	23.768649460564404
10-11	20.705254821455384	31.16895883066077	26.245030187319994	21.88075616056385
12-13	21.618484966456254	25.502921046355116	28.058755289466024	24.819838697722602
14-15	21.07434933136463	26.104442554084468	27.681165281777243	25.140042832773656
16-17	21.617865776893876	26.38119441353069	26.955990435637478	25.044949373937957
18-19	21.351656066899018	26.635062134105013	26.871614754153395	25.14166704484257
20-21	21.64636939767669	26.568486193078616	26.999372894216638	24.785771515028056
22-23	21.632680605642634	26.777662535365977	26.876531694053785	24.7131251649376
24-25	21.568086329593225	26.559552833022888	26.67142164983024	25.20093918755365
26-27	21.415786601046158	26.655520209498217	26.897584139174587	25.031109050281042
28-29	21.421941132209554	26.816680861959053	26.820145210003236	24.941232795828153
30-31	21.469111362914866	26.470327665449737	26.90748594694423	25.153075024691162
32-33	21.574359885625487	26.468787337117693	26.8349230417191	25.121929735537712
34-35	21.725726748490406	26.589975148925976	26.547517524155367	25.136780578428247
36-37	21.499688093055255	26.52809517774385	26.487738126393516	25.484478602807375
38-39	21.60622135546134	26.58547602313825	26.758154145229838	25.05014847617057
40-41	21.790681061230295	26.810906744591545	26.563741685714582	24.834670508463578
42-43	21.851507944737673	26.416006191268597	26.569174616916953	25.16331124707678
44-45	21.75646234691289	26.415760605534995	26.712343261763642	25.115433785788472
46-47	21.701677133713517	26.53988459926378	26.622456270651572	25.135981996371132
48-49	21.596801575009238	26.363486114569557	26.7094223591023	25.330289951318896
50-51	21.556180649625993	26.452535762834618	26.75571396357896	25.23556962396043
52-53	22.069295463386183	26.55902591710661	26.339828893104077	25.03184972640312
54-55	21.906439464331534	26.456799549188197	26.348647772293116	25.288113214187153
56-57	21.74311058210899	26.371291299453947	26.7716378470502	25.113960271386865
58-59	21.744501799163697	26.444010802741648	26.74290954144728	25.068577856647373
60-61	21.679932345833098	26.221704767856295	26.735880302976504	25.362482583334096
62-63	21.77303023978129	26.222514678254345	26.867434571453806	25.13702051051056
64-65	21.95316868168455	26.412142134885663	26.516492558138303	25.11819662529148
66-67	21.780841956201144	26.315803293038204	26.537774913307583	25.365579837453073
68-69	21.800713499709307	26.29413296466212	26.79369745494018	25.111456080688395
70-71	21.980010679844277	26.55277785027782	26.587504718054646	24.879706751823257
72-73	22.010695890196796	26.3946813109675	26.37209133295449	25.222531465881215
74-75	22.003456926186598	26.3234938415893	26.62329301924865	25.049756212975456
76-77	21.895314953122256	26.401980372004385	26.551284741269814	25.15141993360355
78-79	21.788702678536346	26.284808672093746	26.606476219399532	25.32001242997038
80-81	21.894593871606578	26.30863166709422	26.75159125839149	25.04518320290771
82-83	22.01173304130577	26.537206669721858	26.392427348228736	25.058632940743635
84-85	22.09720732705559	26.299948643842917	26.33226406872492	25.270579960376573
86-87	22.036154248302488	26.260243912698495	26.552744246465785	25.150857592533228
88-89	22.2234482644026	26.31939247608409	26.572724693482453	24.884434566030862
90-91	22.088259123464283	26.11722632650848	26.580960119515602	25.213554430511635
92-93	22.00360079599843	26.21959123494621	26.566982629071727	25.209825339983627
94-95	22.234233756424434	26.306797885500284	26.34220458012714	25.116763777948137
96-97	22.213634559388755	26.29603364148334	26.21428624987162	25.27604554925628
98-99	22.123076126430565	26.405597536346626	26.49872286274378	24.97260347447903
100-101	22.21492388449016	26.429444172340677	26.400002623064644	24.95562932010452
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	60.0
1	49.0
2	31.0
3	34.5
4	53.0
5	67.5
6	101.5
7	118.0
8	145.0
9	195.0
10	225.0
11	292.5
12	333.0
13	351.0
14	413.5
15	481.0
16	599.5
17	783.5
18	947.5
19	1149.5
20	1533.0
21	2113.5
22	2995.0
23	4303.0
24	6674.5
25	9948.0
26	14100.5
27	20528.5
28	29876.0
29	43091.5
30	61335.5
31	85087.0
32	119172.5
33	166348.5
34	226612.5
35	312664.5
36	429545.5
37	572893.0
38	755535.5
39	976034.5
40	1215202.5
41	1469644.5
42	1730101.0
43	1973984.5
44	2174904.5
45	2302254.5
46	2374813.5
47	2374755.5
48	2285878.0
49	2145969.0
50	1977120.0
51	1781181.0
52	1607916.0
53	1454426.0
54	1276294.5
55	1095832.5
56	944102.0
57	788608.0
58	611021.0
59	481988.0
60	393274.0
61	330193.0
62	283164.0
63	235238.5
64	209107.0
65	190929.0
66	170122.0
67	146243.0
68	104536.0
69	72080.0
70	58018.5
71	44576.5
72	33515.0
73	30753.0
74	26516.0
75	18473.0
76	11361.5
77	4029.0
78	599.0
79	184.0
80	72.0
81	32.0
82	12.5
83	4.5
84	3.0
85	1.0
86	2.0
87	2.0
88	0.5
89	0.5
90	1.0
91	0.5
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	1.0
98	1.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024636951785694364
2	0.0
3	0.002330451855020082
4	0.0
5	0.0
6	0.0
7	0.0
8	8.203608547940648E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0024610825643821945
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.329732941974179E-4
26-27	0.0
28-29	6.244147907508964E-4
30-31	0.0
32-33	9.144149655347855E-6
34-35	7.445950433640396E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	4.180182699587591E-5
74-75	5.66937278631567E-4
76-77	0.0
78-79	0.0015858568116560424
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0017478388912650614
88-89	3.2657677340528056E-5
90-91	0.0
92-93	3.540092223713241E-4
94-95	0.0015453612917537877
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.8275839E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.31227502503692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.75978152664382	17.202476450434972
2	16.035333346088017	9.07993535625179
3	6.9403357290957395	5.894900817847462
4	3.7987111036502506	4.302006140288297
5	2.3931529059251364	3.3877801624759387
6	1.7320641218717088	2.942320546765852
7	1.2872295461523722	2.551107785171359
8	0.9631515297206744	2.1815208800189403
9	0.7547915101410486	1.9232878339508683
>10	4.7102342889189135	25.075474153411164
>50	0.39137594861831476	7.6321278740970735
>100	0.2129656685041365	11.20538159658314
>500	0.014105319358648491	2.6958666054400315
>1k	0.006504137142821868	3.336369296356746
>5k	2.3406059392444616E-4	0.4777306625902437
>10k+	2.925757424055577E-5	0.11171383831589009
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.180182699587591E-5	0.0	0.0	0.0	0.0
2	6.531535468105611E-5	0.0	0.0	0.0	2.6126141872422443E-6
3	8.360365399175182E-5	0.0	0.0	0.0	5.225228374484489E-6
4	1.1756763842590099E-4	7.837842561726733E-6	0.0	0.0	1.0450456748968977E-5
5	1.4630639448556568E-4	7.837842561726733E-6	0.0	0.0	1.0450456748968977E-5
6	1.698199221707459E-4	7.837842561726733E-6	2.6126141872422444E-5	0.0	1.0450456748968977E-5
7	1.933334498559261E-4	7.837842561726733E-6	3.135137024690693E-5	0.0	1.3063070936211222E-5
8	2.299100484773175E-4	7.837842561726733E-6	3.135137024690693E-5	0.0	1.3063070936211222E-5
9	2.481983477880132E-4	7.837842561726733E-6	3.135137024690693E-5	0.0	1.828829931069571E-5
10-11	2.6779295419233006E-4	2.6126141872422444E-5	3.265767734052805E-5	0.0	2.2207220591559077E-5
12-13	3.0306324572010036E-4	4.180182699587591E-5	3.3963984434149175E-5	0.0	2.8738756059664685E-5
14-15	3.762164429628832E-4	4.572074827673928E-5	3.3963984434149175E-5	0.0	3.135137024690693E-5
16-17	4.624327111418773E-4	4.70270553703604E-5	3.3963984434149175E-5	0.0	3.3963984434149175E-5
18-19	5.264417587293122E-4	4.70270553703604E-5	3.52702915277703E-5	0.0	3.52702915277703E-5
20-21	6.296400191253809E-4	4.70270553703604E-5	3.657659862139142E-5	0.0	3.788290571501254E-5
22-23	6.975679879936792E-4	4.70270553703604E-5	3.657659862139142E-5	0.0	4.049551990225479E-5
24-25	8.177482406068225E-4	5.094597665122377E-5	3.657659862139142E-5	0.0	4.180182699587591E-5
26-27	9.692798634668726E-4	8.882888236623631E-5	3.657659862139142E-5	0.0	4.180182699587591E-5
28-29	0.001218784518348507	9.666672492796305E-5	4.180182699587591E-5	0.0	4.180182699587591E-5
30-31	0.0015009468505706694	9.666672492796305E-5	4.4414441183118154E-5	0.0	4.4414441183118154E-5
32-33	0.0019503164907763355	9.666672492796305E-5	4.70270553703604E-5	0.0	4.4414441183118154E-5
34-35	0.0025433799112803248	9.927933911520529E-5	4.70270553703604E-5	0.0	4.572074827673928E-5
36-37	0.003359821844793526	1.0450456748968978E-4	4.70270553703604E-5	0.0	4.70270553703604E-5
38-39	0.0043826602990988655	1.0711718167693201E-4	4.70270553703604E-5	0.0	5.747751211932937E-5
40-41	0.00590058914188661	1.0711718167693201E-4	4.70270553703604E-5	0.0	6.792796886829835E-5
42-43	0.007895320073846062	1.2148655970676436E-4	4.833336246398152E-5	0.0	6.792796886829835E-5
44-45	0.010257123299113052	1.4761270157918682E-4	5.094597665122377E-5	0.0	6.923427596191948E-5
46-47	0.012854061801231843	1.8288299310695712E-4	5.225228374484489E-5	0.0	7.315319724278284E-5
48-49	0.01634190174120024	1.8288299310695712E-4	5.486489793208713E-5	0.0	8.360365399175183E-5
50-51	0.020804246773009995	1.8549560729419934E-4	5.486489793208713E-5	1.3063070936211222E-6	8.882888236623631E-5
52-53	0.02662253856799847	1.946397569495472E-4	5.486489793208713E-5	2.6126141872422443E-6	8.882888236623631E-5
54-55	0.03306263253955061	1.9594606404316832E-4	5.486489793208713E-5	2.6126141872422443E-6	9.666672492796304E-5
56-57	0.04030871798786697	2.416668123199076E-4	7.05405830555406E-5	2.6126141872422443E-6	1.058108745833109E-4
58-59	0.048875479907834286	2.495046548816344E-4	9.144149655347856E-5	2.6126141872422443E-6	1.0972979586417427E-4
60-61	0.05971521617070236	2.5734249744336106E-4	9.144149655347856E-5	2.6126141872422443E-6	1.1103610295779538E-4
62-63	0.07377108049806563	2.5864880453698217E-4	9.144149655347856E-5	2.6126141872422443E-6	1.123424100514165E-4
64-65	0.09092158633021735	2.612614187242244E-4	9.144149655347856E-5	2.6126141872422443E-6	1.1756763842590099E-4
66-67	0.110616778380743	2.743244896604357E-4	9.144149655347856E-5	2.6126141872422443E-6	1.2018025261314324E-4
68-69	0.13433147735834086	2.743244896604357E-4	9.144149655347856E-5	2.6126141872422443E-6	1.3063070936211222E-4
70-71	0.16238964742222894	2.743244896604357E-4	9.144149655347856E-5	2.6126141872422443E-6	1.3193701645573336E-4
72-73	0.19780885795867204	2.743244896604357E-4	9.927933911520529E-5	2.6126141872422443E-6	1.3324332354935447E-4
74-75	0.24134937969615766	2.7824341094129907E-4	9.927933911520529E-5	2.6126141872422443E-6	1.3324332354935447E-4
76-77	0.29561207005808543	3.448650727159762E-4	9.927933911520529E-5	2.6126141872422443E-6	1.3324332354935447E-4
78-79	0.35949832477872007	3.6576598621391424E-4	9.927933911520529E-5	2.6126141872422443E-6	1.3324332354935447E-4
80-81	0.43560638866727386	3.6576598621391424E-4	9.927933911520529E-5	2.6126141872422443E-6	1.410811661110812E-4
82-83	0.5263281100121673	3.6576598621391424E-4	9.927933911520529E-5	2.6126141872422443E-6	1.5153162286005018E-4
84-85	0.6377156095781467	3.709912145883987E-4	9.927933911520529E-5	2.6126141872422443E-6	1.5153162286005018E-4
86-87	0.775907224398138	3.892795138990944E-4	9.927933911520529E-5	2.6126141872422443E-6	1.5153162286005018E-4
88-89	0.9411981798752994	4.1801826995875905E-4	9.927933911520529E-5	2.6126141872422443E-6	1.5153162286005018E-4
90	1.0830200221084638	4.5720748276739274E-4	1.0189195330244752E-4	2.6126141872422443E-6	1.5153162286005018E-4
>>END_MODULE
