##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654118_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24512826
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.648206004481082	32.0	32.0	32.0	32.0	32.0
2	31.614521801770223	32.0	32.0	32.0	32.0	32.0
3	35.70615815573447	37.0	37.0	37.0	32.0	37.0
4	36.334757444939235	37.0	37.0	37.0	37.0	37.0
5	36.51209338327617	37.0	37.0	37.0	37.0	37.0
6	40.097676620394566	41.0	41.0	41.0	37.0	41.0
7	40.14285806948575	41.0	41.0	41.0	37.0	41.0
8	40.23075821612734	41.0	41.0	41.0	37.0	41.0
9	40.24121413010479	41.0	41.0	41.0	41.0	41.0
10-11	40.293909115170976	41.0	41.0	41.0	41.0	41.0
12-13	40.25733444605693	41.0	41.0	41.0	41.0	41.0
14-15	40.20630104011671	41.0	41.0	41.0	41.0	41.0
16-17	40.23169854018464	41.0	41.0	41.0	41.0	41.0
18-19	40.228075885660836	41.0	41.0	41.0	41.0	41.0
20-21	40.208441511394895	41.0	41.0	41.0	41.0	41.0
22-23	40.19805751894947	41.0	41.0	41.0	41.0	41.0
24-25	40.18000125730097	41.0	41.0	41.0	41.0	41.0
26-27	40.12914912380971	41.0	41.0	41.0	39.0	41.0
28-29	40.108249289575994	41.0	41.0	41.0	37.0	41.0
30-31	40.084025093638736	41.0	41.0	41.0	37.0	41.0
32-33	40.078032536925775	41.0	41.0	41.0	37.0	41.0
34-35	40.06521035151149	41.0	41.0	41.0	37.0	41.0
36-37	39.96017947910208	41.0	41.0	41.0	37.0	41.0
38-39	39.99576338117849	41.0	41.0	41.0	37.0	41.0
40-41	40.00280542928833	41.0	41.0	41.0	37.0	41.0
42-43	39.982384854361555	41.0	41.0	41.0	37.0	41.0
44-45	39.97140445985298	41.0	41.0	41.0	37.0	41.0
46-47	39.94907594497673	41.0	41.0	41.0	37.0	41.0
48-49	39.92935486100215	41.0	41.0	41.0	37.0	41.0
50-51	39.86440855493365	41.0	41.0	41.0	37.0	41.0
52-53	39.86163041748022	41.0	41.0	41.0	37.0	41.0
54-55	39.8193516732832	41.0	41.0	41.0	37.0	41.0
56-57	39.80473287739243	41.0	41.0	41.0	37.0	41.0
58-59	39.77136104176646	41.0	41.0	41.0	37.0	41.0
60-61	39.76539665398025	41.0	41.0	41.0	37.0	41.0
62-63	39.71602248145522	41.0	41.0	41.0	37.0	41.0
64-65	39.7191108646551	41.0	41.0	41.0	37.0	41.0
66-67	39.66002734650016	41.0	41.0	41.0	37.0	41.0
68-69	39.62266623195546	41.0	41.0	41.0	37.0	41.0
70-71	39.61574301959309	41.0	41.0	41.0	37.0	41.0
72-73	39.583917864060226	41.0	41.0	41.0	37.0	41.0
74-75	39.538117881634705	41.0	41.0	41.0	37.0	41.0
76-77	38.918825169321565	41.0	39.0	41.0	34.5	41.0
78-79	39.304429016058776	41.0	41.0	41.0	37.0	41.0
80-81	39.614242764175785	41.0	41.0	41.0	37.0	41.0
82-83	39.639818069120224	41.0	41.0	41.0	37.0	41.0
84-85	39.65158894776147	41.0	41.0	41.0	37.0	41.0
86-87	39.63195722516857	41.0	41.0	41.0	37.0	41.0
88-89	39.58224410763573	41.0	41.0	41.0	37.0	41.0
90-91	39.542190749446846	41.0	41.0	41.0	37.0	41.0
92-93	39.490689425201325	41.0	41.0	41.0	37.0	41.0
94-95	39.45976031078587	41.0	41.0	41.0	37.0	41.0
96-97	39.433760697359006	41.0	41.0	41.0	37.0	41.0
98-99	39.37965073876019	41.0	41.0	41.0	37.0	41.0
100-101	38.824597620853666	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	1.0
16	3.0
17	17.0
18	51.0
19	145.0
20	541.0
21	1397.0
22	3446.0
23	7173.0
24	13179.0
25	23025.0
26	38071.0
27	59304.0
28	85937.0
29	118167.0
30	154153.0
31	194991.0
32	240193.0
33	293349.0
34	349901.0
35	422634.0
36	527209.0
37	696314.0
38	1050401.0
39	2378242.0
40	1.785498E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.807243971636936	12.680308596629128	8.512595797852061	51.999851633881875
2	22.360392065769975	16.40700668295039	36.29133172976465	24.94126952151498
3	19.69273063845814	20.079458870940893	23.244918143965577	36.98289234663539
4	22.611929770969695	27.684111166945826	20.978433902317096	28.72552515976738
5	23.19044323979618	31.301596968052564	24.63748161880642	20.870478173344846
6	18.627248445364888	32.258349159741925	26.062360170141137	23.052042224752054
7	15.624036983740675	22.150571296838642	41.60049926516021	20.624892454260475
8	18.811812805477864	22.854579512699154	31.048073647291623	27.285534034531356
9	18.272479068712844	21.575492764481748	34.13778566371744	26.014242503087974
10-11	22.01482399458961	29.7518735701873	23.3510999506952	24.882202484527895
12-13	22.098308860838813	23.404441413650144	26.63633519856095	27.860914526950097
14-15	20.76607538884072	26.196786647901604	27.635717806406944	25.401420156850733
16-17	21.77232033548478	26.31484839814063	26.49014234425684	25.422688922117754
18-19	21.427812933523047	26.596539705377097	26.37597558111007	25.59967177998979
20-21	21.711919302980405	26.43325783816195	26.65599837407568	25.198824484781966
22-23	21.49129194650996	26.71928565070384	26.684897530786532	25.10452487199966
24-25	21.34886156023613	26.393949730575304	26.657951726112838	25.59923698307573
26-27	21.299327135924678	26.52506079878346	26.9479149405295	25.22769712476236
28-29	21.427266127162092	26.72404637037872	26.63746667055853	25.211220831900654
30-31	21.523772901582216	26.356400114780726	26.616492525178455	25.503334458458603
32-33	21.660742624380717	26.433962746763196	26.698252991913662	25.207041636942428
34-35	21.66643658286721	26.649429555357347	26.39394474921029	25.29018911256515
36-37	21.53756568092149	26.376514074713377	26.417107109559705	25.66881313480543
38-39	21.753515894087446	26.519441291673186	26.56427700339406	25.16276581084531
40-41	21.827550605548296	26.69369700580423	26.346097345120466	25.13265504352701
42-43	21.742958155865015	26.17479722656213	26.48126331904775	25.600981298525106
44-45	21.750774472106972	26.357148702479265	26.705794754142175	25.186282071271588
46-47	21.778205009899715	26.47292890668746	26.46700955654807	25.281856526864754
48-49	21.703205497399605	26.25071054638906	26.368455028400234	25.677628927811096
50-51	21.856831195228164	26.3085720920142	26.554033386440224	25.280563326317413
52-53	22.211219138911197	26.44194104751529	26.137934891717503	25.20890492185601
54-55	21.847064879422714	26.270026964659237	26.268456358316257	25.614451797601795
56-57	21.8434973593008	26.226904641676157	26.74611242294136	25.183485576081683
58-59	21.93052526868995	26.30696273044977	26.462891304331865	25.299620696528418
60-61	21.911084425761437	26.05717920895779	26.434995703881714	25.59674066139906
62-63	22.03414857185377	26.10125001499215	26.586192061249896	25.278409351904184
64-65	22.024186440192576	26.39932662190806	26.34384750252786	25.232639435371507
66-67	21.94694320434535	26.159439960125365	26.368049118449257	25.525567717080026
68-69	22.048159604282265	26.16868614006398	26.62405591260673	25.159098343047027
70-71	22.256587224989886	26.369988919270266	26.329587620782686	25.043836234957162
72-73	22.097361019500877	26.12111291894076	26.259917846656432	25.52160821490193
74-75	22.099155343551065	26.128113734405627	26.51674920351452	25.255981718528787
76-77	22.095895838366413	26.33837689705789	26.341410003073495	25.224317261502204
78-79	22.03418335593989	26.085420388715903	26.367303946836923	25.51309230850728
80-81	22.21583101026377	26.159101361874797	26.520761008950988	25.104306618910442
82-83	22.281527229867333	26.310418064404324	26.140792579362333	25.267262126366006
84-85	22.259679483711913	26.107183643371023	26.200697952981837	25.43243891993522
86-87	22.316998788411453	26.077702483150706	26.43887001571079	25.166428712727047
88-89	22.45059665528405	26.150102554525155	26.293857979748875	25.105442810441918
90-91	22.32689939544302	25.955632369764302	26.302940346412935	25.41452788837974
92-93	22.351749215112125	26.019887130515045	26.32709884661186	25.30126480776096
94-95	22.517514529207617	26.198444303849133	26.131455853558734	25.152585313384517
96-97	22.406329241679437	26.16039456242214	25.97318440558424	25.46009179031418
98-99	22.516153380275288	26.232360968906644	26.304284540672707	24.947201110145357
100-101	22.570596307418818	26.379092316814063	26.01804051479009	25.032270860977025
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	28.0
1	32.0
2	26.5
3	20.5
4	37.5
5	53.5
6	66.5
7	81.5
8	96.5
9	125.0
10	149.0
11	187.0
12	221.0
13	254.0
14	310.0
15	351.0
16	419.5
17	519.5
18	627.5
19	805.0
20	1064.0
21	1480.0
22	2094.0
23	3116.0
24	5064.0
25	7440.5
26	10429.5
27	15349.0
28	22158.5
29	31906.0
30	45775.0
31	62688.5
32	85033.0
33	115103.5
34	153122.0
35	203965.0
36	269320.0
37	348619.0
38	455123.0
39	593887.0
40	750131.5
41	916741.0
42	1090380.0
43	1254134.5
44	1388380.0
45	1477469.5
46	1522716.5
47	1520715.5
48	1466625.0
49	1376642.0
50	1270699.0
51	1147762.5
52	1033773.0
53	934500.5
54	819458.0
55	700276.0
56	588997.0
57	485072.5
58	391501.5
59	311605.5
60	254745.5
61	223531.0
62	196713.5
63	168541.5
64	149045.0
65	135909.5
66	118392.0
67	98981.5
68	74054.5
69	51911.5
70	41679.0
71	32456.0
72	23197.0
73	18408.5
74	16000.5
75	12861.5
76	8299.0
77	2747.0
78	409.5
79	129.0
80	47.0
81	20.5
82	9.5
83	6.5
84	4.0
85	1.5
86	1.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02399968082015513
2	0.0
3	0.0021294974312631274
4	0.0
5	0.0
6	0.0
7	0.0
8	8.444558779146885E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0024640161848331972
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.405333518052957E-4
26-27	0.0
28-29	6.180437947056778E-4
30-31	0.0
32-33	1.2238490984270846E-5
34-35	7.343094590562509E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	3.0596227460677114E-5
74-75	5.62970585276459E-4
76-77	0.0
78-79	0.001629759049405401
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0015461293610128835
88-89	4.895396393708338E-5
90-91	0.0
92-93	3.6307523253336846E-4
94-95	0.0015971230734473456
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.4512826E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.00430185595026
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.97090366190994	20.78310712599639
2	15.722409412868899	10.378142923303177
3	6.60019425015347	6.535044100199173
4	3.6295598000481855	4.791643489800511
5	2.3663110915677024	3.90492227755918
6	1.6354260502244593	3.2385657014815514
7	1.1559234034821093	2.6705311451616645
8	0.8642681950537223	2.281965471524028
9	0.6951524767762591	2.0648719961491597
>10	3.9191369620320966	23.757106633489443
>50	0.27810834015359454	6.298637517574343
>100	0.14945578139441712	8.976738335665267
>500	0.00916720606290496	2.0441016174657016
>1k	0.003970442505141869	2.2484067311743567
>5k	1.292576696591295E-5	0.026214933456020544
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6317987979027795E-5	0.0	0.0	0.0	0.0
2	2.4476981968541695E-5	4.079496994756949E-6	4.079496994756949E-6	0.0	0.0
3	6.935144891086813E-5	4.079496994756949E-6	4.079496994756949E-6	0.0	1.2238490984270847E-5
4	1.1422591585319456E-4	4.079496994756949E-6	8.158993989513898E-6	0.0	1.6317987979027795E-5
5	1.346234008269793E-4	4.079496994756949E-6	8.158993989513898E-6	0.0	1.6317987979027795E-5
6	1.6317987979027796E-4	4.079496994756949E-6	8.158993989513898E-6	0.0	1.6317987979027795E-5
7	1.7949786776930575E-4	8.158993989513898E-6	8.158993989513898E-6	0.0	1.6317987979027795E-5
8	2.1621334072211828E-4	8.158993989513898E-6	8.158993989513898E-6	0.0	1.6317987979027795E-5
9	2.243723347116322E-4	8.158993989513898E-6	8.158993989513898E-6	0.0	1.6317987979027795E-5
10-11	2.7332629864871557E-4	1.6317987979027795E-5	1.0198742486892373E-5	0.0	2.447698196854169E-5
12-13	3.263597595805559E-4	3.263597595805559E-5	1.2238490984270847E-5	0.0	2.855647896329864E-5
14-15	4.303869329468581E-4	3.671547295281254E-5	1.2238490984270847E-5	0.0	2.855647896329864E-5
16-17	5.119768728419971E-4	3.8755221450191014E-5	1.2238490984270847E-5	0.0	3.467572445543407E-5
18-19	5.97646309731893E-4	4.079496994756949E-5	1.2238490984270847E-5	0.0	3.671547295281254E-5
20-21	7.281902135641154E-4	4.283471844494796E-5	1.6317987979027795E-5	4.079496994756949E-6	4.079496994756949E-5
22-23	8.199788959461468E-4	5.5073209429218806E-5	1.835773647640627E-5	4.079496994756949E-6	4.079496994756949E-5
24-25	9.668407877573969E-4	5.711295792659728E-5	2.0397484973784745E-5	4.079496994756949E-6	4.079496994756949E-5
26-27	0.0011606168950083519	7.343094590562507E-5	2.0397484973784745E-5	4.079496994756949E-6	4.283471844494796E-5
28-29	0.0014053867146937688	7.343094590562507E-5	2.4476981968541695E-5	4.079496994756949E-6	4.895396393708339E-5
30-31	0.0017521439592481096	7.343094590562507E-5	2.855647896329864E-5	4.079496994756949E-6	4.895396393708339E-5
32-33	0.0021437756707447767	7.95501913977605E-5	2.855647896329864E-5	4.079496994756949E-6	4.895396393708339E-5
34-35	0.002825051668869187	8.566943688989592E-5	2.855647896329864E-5	4.079496994756949E-6	4.895396393708339E-5
36-37	0.003632792073831063	8.566943688989592E-5	2.855647896329864E-5	4.079496994756949E-6	5.3033460931840336E-5
38-39	0.004801567962828929	8.77091853872744E-5	2.855647896329864E-5	4.079496994756949E-6	5.5073209429218806E-5
40-41	0.006482320724668792	9.382843087940983E-5	2.855647896329864E-5	4.079496994756949E-6	5.711295792659728E-5
42-43	0.008746441556758898	1.0810667036105915E-4	2.855647896329864E-5	4.079496994756949E-6	5.711295792659728E-5
44-45	0.01136547862739286	1.2238490984270846E-4	3.263597595805559E-5	4.079496994756949E-6	5.711295792659728E-5
46-47	0.014194609793256804	1.3870289782173627E-4	3.263597595805559E-5	4.079496994756949E-6	5.711295792659728E-5
48-49	0.017980383004391254	1.448221433138717E-4	3.671547295281254E-5	4.079496994756949E-6	6.323220341873271E-5
50-51	0.023563174641716138	1.59100382795521E-4	4.079496994756949E-5	4.079496994756949E-6	6.527195191611118E-5
52-53	0.03068597639456177	1.6521962828765642E-4	4.079496994756949E-5	6.119245492135423E-6	6.935144891086813E-5
54-55	0.038702187989259174	1.672593767850349E-4	5.711295792659728E-5	8.158993989513898E-6	7.343094590562507E-5
56-57	0.04703252085255286	2.080543467326044E-4	6.527195191611118E-5	8.158993989513898E-6	7.751044290038203E-5
58-59	0.056052288707960474	2.080543467326044E-4	6.935144891086813E-5	8.158993989513898E-6	8.362968839251745E-5
60-61	0.067071010090799	2.080543467326044E-4	6.935144891086813E-5	8.158993989513898E-6	8.566943688989592E-5
62-63	0.08074956351421905	2.080543467326044E-4	6.935144891086813E-5	8.158993989513898E-6	8.77091853872744E-5
64-65	0.09736739452236148	2.100940952299829E-4	6.935144891086813E-5	8.158993989513898E-6	8.974893388465287E-5
66-67	0.1163370555479813	2.2029283771687525E-4	6.935144891086813E-5	8.158993989513898E-6	9.178868238203134E-5
68-69	0.138254153152313	2.2029283771687525E-4	6.935144891086813E-5	8.158993989513898E-6	9.994767637154525E-5
70-71	0.1632329132512098	2.2029283771687525E-4	6.935144891086813E-5	8.158993989513898E-6	1.0606692186368067E-4
72-73	0.1929948835764591	2.2233258621425373E-4	6.935144891086813E-5	8.158993989513898E-6	1.121861673558161E-4
74-75	0.23018357818066346	2.610878076644447E-4	6.935144891086813E-5	8.158993989513898E-6	1.1422591585319456E-4
76-77	0.27624925824545893	2.9168403512512184E-4	6.935144891086813E-5	8.158993989513898E-6	1.1422591585319456E-4
78-79	0.331016505400071	2.937237836225003E-4	6.935144891086813E-5	8.158993989513898E-6	1.1422591585319456E-4
80-81	0.3941977151063692	2.937237836225003E-4	6.935144891086813E-5	8.158993989513898E-6	1.2442465834008694E-4
82-83	0.4676225417665022	2.998430291146357E-4	7.13911974082466E-5	8.158993989513898E-6	1.3054390383222236E-4
84-85	0.5566942791500253	3.243200110831774E-4	7.343094590562507E-5	8.158993989513898E-6	1.3054390383222236E-4
86-87	0.6682705617051252	3.4471749605696214E-4	7.343094590562507E-5	8.158993989513898E-6	1.3054390383222236E-4
88-89	0.8048663993290697	3.6307523253336846E-4	7.343094590562507E-5	8.158993989513898E-6	1.427823948164932E-4
90	0.9236715505588788	3.834727175071532E-4	8.158993989513898E-5	8.158993989513898E-6	1.4686189181125015E-4
>>END_MODULE
