##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654121_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24804838
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.646161365778724	32.0	32.0	32.0	32.0	32.0
2	31.6153276630954	32.0	32.0	32.0	32.0	32.0
3	35.71304501162233	37.0	37.0	37.0	32.0	37.0
4	36.33719784825847	37.0	37.0	37.0	37.0	37.0
5	36.516453362848004	37.0	37.0	37.0	37.0	37.0
6	40.103401118765625	41.0	41.0	41.0	37.0	41.0
7	40.14826361696053	41.0	41.0	41.0	37.0	41.0
8	40.235462775447274	41.0	41.0	41.0	37.0	41.0
9	40.24865153322106	41.0	41.0	41.0	41.0	41.0
10-11	40.299197479136936	41.0	41.0	41.0	41.0	41.0
12-13	40.2657802481919	41.0	41.0	41.0	41.0	41.0
14-15	40.21429779142279	41.0	41.0	41.0	41.0	41.0
16-17	40.23895854510317	41.0	41.0	41.0	41.0	41.0
18-19	40.2355029490618	41.0	41.0	41.0	41.0	41.0
20-21	40.21374892672147	41.0	41.0	41.0	41.0	41.0
22-23	40.205998321779006	41.0	41.0	41.0	41.0	41.0
24-25	40.18893050218671	41.0	41.0	41.0	41.0	41.0
26-27	40.13894607979298	41.0	41.0	41.0	41.0	41.0
28-29	40.11891339504011	41.0	41.0	41.0	39.0	41.0
30-31	40.092156981633984	41.0	41.0	41.0	37.0	41.0
32-33	40.08568064423561	41.0	41.0	41.0	37.0	41.0
34-35	40.07675879600585	41.0	41.0	41.0	37.0	41.0
36-37	39.975317395743524	41.0	41.0	41.0	37.0	41.0
38-39	40.014928196668734	41.0	41.0	41.0	37.0	41.0
40-41	40.01717233549358	41.0	41.0	41.0	37.0	41.0
42-43	40.00585055221889	41.0	41.0	41.0	37.0	41.0
44-45	39.98786976556751	41.0	41.0	41.0	37.0	41.0
46-47	39.96842541362294	41.0	41.0	41.0	37.0	41.0
48-49	39.94389227617613	41.0	41.0	41.0	37.0	41.0
50-51	39.88472506855316	41.0	41.0	41.0	37.0	41.0
52-53	39.88088315674547	41.0	41.0	41.0	37.0	41.0
54-55	39.8402312484363	41.0	41.0	41.0	37.0	41.0
56-57	39.828708879292016	41.0	41.0	41.0	37.0	41.0
58-59	39.792250406956896	41.0	41.0	41.0	37.0	41.0
60-61	39.79476606539418	41.0	41.0	41.0	37.0	41.0
62-63	39.740732815912764	41.0	41.0	41.0	37.0	41.0
64-65	39.74620142651204	41.0	41.0	41.0	37.0	41.0
66-67	39.68790918932831	41.0	41.0	41.0	37.0	41.0
68-69	39.653477398239815	41.0	41.0	41.0	37.0	41.0
70-71	39.653666917719846	41.0	41.0	41.0	37.0	41.0
72-73	39.63178253774525	41.0	41.0	41.0	37.0	41.0
74-75	39.58864359444718	41.0	41.0	41.0	37.0	41.0
76-77	39.02825317786796	41.0	39.0	41.0	34.5	41.0
78-79	39.39258982461405	41.0	41.0	41.0	37.0	41.0
80-81	39.676352955822566	41.0	41.0	41.0	37.0	41.0
82-83	39.69944228218705	41.0	41.0	41.0	37.0	41.0
84-85	39.71474830837436	41.0	41.0	41.0	37.0	41.0
86-87	39.69377540381437	41.0	41.0	41.0	37.0	41.0
88-89	39.64881099001735	41.0	41.0	41.0	37.0	41.0
90-91	39.61475624231047	41.0	41.0	41.0	37.0	41.0
92-93	39.56599385974623	41.0	41.0	41.0	37.0	41.0
94-95	39.544181804372194	41.0	41.0	41.0	37.0	41.0
96-97	39.52248131997476	41.0	41.0	41.0	37.0	41.0
98-99	39.475056781261785	41.0	41.0	41.0	37.0	41.0
100-101	38.939791322160616	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	17.0
18	40.0
19	143.0
20	433.0
21	1207.0
22	2915.0
23	6523.0
24	12115.0
25	21425.0
26	35702.0
27	56440.0
28	82605.0
29	114661.0
30	151194.0
31	191575.0
32	237050.0
33	288715.0
34	346879.0
35	421040.0
36	526905.0
37	694266.0
38	1041905.0
39	2326655.0
40	1.8244426E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.050613285105243	13.568239624256393	7.947703167387971	48.433443923250394
2	22.774436180554776	16.12741433747723	34.421365702932626	26.67678377903536
3	19.656210318109046	19.00467390866573	22.415994682855906	38.923121090369314
4	22.60201820306184	26.257925973957175	21.03398538623796	30.106070436743025
5	23.142465191669466	29.848531967836273	24.19873494033704	22.810267900157218
6	19.27323613240288	31.080848018438985	25.866538616377984	23.779377232780153
7	15.803408996261131	22.97350218533981	40.321686438750376	20.90140237964868
8	18.537886144145	23.418722657861654	31.024143767812678	27.019247430180666
9	18.15782469532758	22.19824616471996	33.66937933640203	25.974549803550424
10-11	21.702028451062652	29.80731420217298	23.598102112176665	24.892555234587704
12-13	21.97719452955105	23.769411434978934	26.481071958623552	27.77232207684646
14-15	20.785442406766183	25.87063803110255	27.353612462386717	25.99030709974455
16-17	21.670709963919137	26.20975593551548	26.28787980796327	25.831654292602114
18-19	21.399692269709643	26.329071369061147	26.405487913285302	25.865748447943904
20-21	21.632707296858783	26.280453837271583	26.634967339839104	25.45187152603053
22-23	21.593015443197007	26.515924030626607	26.45984021342933	25.431220312747055
24-25	21.351755508505796	26.262383864030646	26.404288379534407	25.981572247929147
26-27	21.30428950997382	26.187917453845095	26.824511008699194	25.683282027481898
28-29	21.474170107767762	26.427031526286683	26.526335361330883	25.572463004614672
30-31	21.523105694139183	25.959788570278103	26.606853872619528	25.910251862963186
32-33	21.62207445399104	26.029457038729404	26.53152648401962	25.81694202325994
34-35	21.667615575079317	26.239878604226778	26.259612674029952	25.83289314666395
36-37	21.511795400558555	26.173250960155436	26.282675178124528	26.03227846116149
38-39	21.885014931361376	26.22856879774825	26.406386931452648	25.48002933943773
40-41	22.041859334054106	26.26736969618588	26.194468595199048	25.496302374560965
42-43	21.92116916869201	25.80797745988101	26.23392662350788	26.036926747919097
44-45	21.773163767487617	25.935136121429213	26.482065716373555	25.80963439470961
46-47	21.758676674284267	26.082605336910486	26.432674142036323	25.726043846768924
48-49	21.727646437360324	25.809338081546834	26.285563727527673	26.177451753565173
50-51	21.94057465725033	25.909655205166025	26.291941515602723	25.85782862198092
52-53	22.410124186257534	26.034509880693435	25.95981275910772	25.595553173941312
54-55	22.053292587518612	25.861323907860235	26.022008287254284	26.06337521736687
56-57	21.83596603211035	25.743707739594996	26.56301766615045	25.857308562144212
58-59	21.793280810783767	25.815183715370367	26.555853741112923	25.835681732732947
60-61	21.889260070958738	25.514847143932162	26.403597153103764	26.192295632005337
62-63	22.14315812100849	25.555998390314016	26.457197583793935	25.843645904883555
64-65	22.116012206973494	25.919471838517953	26.091359677495173	25.873156277013383
66-67	22.062351304209287	25.701228123320135	26.181303824681297	26.05511674778928
68-69	22.005412815032294	25.694346401294784	26.506276719081978	25.793964064590945
70-71	22.246347668144416	25.90002805097941	26.315223264106784	25.53840101676939
72-73	22.229070126336676	25.68380426845866	26.16307983863789	25.92404576656677
74-75	22.155982076942205	25.481412874400878	26.395584529639066	25.967020519017854
76-77	22.079047643850767	25.708228773757764	26.223837865822787	25.988885716568678
78-79	22.02089227935251	25.576660081564317	26.325643914077755	26.076803725005416
80-81	22.109819866592154	25.628060945207544	26.499771939651453	25.76234724854885
82-83	22.2675108783214	25.780438477364783	25.962997621673644	25.989053022640178
84-85	22.40584276341575	25.547729842057425	25.961191522395755	26.085235872131072
86-87	22.366343431395116	25.461713895461415	26.352151611627438	25.819791061516028
88-89	22.520510218352225	25.689271980929846	26.236504090674917	25.553713710043013
90-91	22.3896947039122	25.465304389409837	26.20109633451345	25.943904572164513
92-93	22.314073792347905	25.43876721048649	26.26156111569502	25.985597881470586
94-95	22.539188683722564	25.527037815181952	26.00568921374774	25.928084287347748
96-97	22.42357317552326	25.639028563701967	25.911259730863794	26.02613852991098
98-99	22.489213596154105	25.639611111348522	26.248734218703625	25.622441073793752
100-101	22.487538519703293	25.893093919823222	25.923592002495642	25.69577555797784
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	63.0
1	45.0
2	26.0
3	30.0
4	42.5
5	44.0
6	58.5
7	92.5
8	99.5
9	110.0
10	129.5
11	167.0
12	217.0
13	242.5
14	287.5
15	380.5
16	481.5
17	539.5
18	636.5
19	789.5
20	1048.0
21	1443.0
22	2061.0
23	3048.0
24	4734.0
25	6877.0
26	9639.5
27	14482.0
28	21996.0
29	31721.0
30	45769.5
31	63706.5
32	90113.0
33	124323.5
34	163030.0
35	214350.5
36	277650.0
37	347002.5
38	434518.5
39	539054.0
40	659051.5
41	812736.5
42	979578.0
43	1129763.0
44	1268582.5
45	1380817.0
46	1449680.0
47	1470102.5
48	1451506.0
49	1393945.0
50	1309632.0
51	1216414.5
52	1110569.0
53	1020112.5
54	920964.5
55	789049.5
56	680484.5
57	598176.0
58	492440.5
59	388499.0
60	317661.0
61	270614.0
62	228936.5
63	187858.5
64	162592.0
65	147982.5
66	126536.5
67	105197.0
68	79635.5
69	57021.0
70	48816.0
71	39383.0
72	28834.5
73	23914.5
74	21503.5
75	17973.0
76	12140.0
77	4203.5
78	630.0
79	174.0
80	59.0
81	26.0
82	12.0
83	4.0
84	1.0
85	1.5
86	1.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024241238745441512
2	0.0
3	0.0021971520233270625
4	0.0
5	0.0
6	0.0
7	0.0
8	7.09539002028556E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0024208986972622036
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.261070441177644E-4
26-27	0.0
28-29	6.309253057810738E-4
30-31	0.0
32-33	1.0078679006087441E-5
34-35	8.264516784991702E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	2.4188829614609858E-5
74-75	5.724689675457666E-4
76-77	0.0
78-79	0.0014674556632863313
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0016448404137934705
88-89	2.8220301217044838E-5
90-91	0.0
92-93	3.406593504057555E-4
94-95	0.0014352038904668516
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.4804838E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.59855744795809
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.70460951122436	17.967688719738376
2	17.019562319268864	10.075089860919647
3	7.260497709769688	6.447007756901588
4	3.8224649323757243	4.525577915749125
5	2.3550253003037582	3.4852675821217773
6	1.587458261819353	2.8191884735217485
7	1.1469738254069195	2.376413946382767
8	0.842087727036589	1.9939665571930345
9	0.6718294815760074	1.789666515509342
>10	4.0282460656640895	22.26477612465351
>50	0.3337793227732676	6.8397463760176995
>100	0.2035348483722159	11.36514793000787
>500	0.016337676143216365	3.3150044853651375
>1k	0.007136586413733789	3.6547262436132884
>5k	3.708508799499758E-4	0.7070989750847375
>10k+	8.558097229614825E-5	0.3736325372204239
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.225177281947981E-5	0.0	0.0	4.031471602434976E-6	4.031471602434976E-6
2	4.434618762678474E-5	0.0	4.031471602434976E-6	4.031471602434976E-6	8.062943204869953E-6
3	5.240913083165469E-5	0.0	4.031471602434976E-6	4.031471602434976E-6	8.062943204869953E-6
4	8.46609036511345E-5	0.0	3.225177281947981E-5	4.031471602434976E-6	1.2094414807304929E-5
5	1.0884973326574436E-4	0.0	3.225177281947981E-5	4.031471602434976E-6	1.6125886409739905E-5
6	1.2497561967548428E-4	0.0	4.8377659229219716E-5	4.031471602434976E-6	1.6125886409739905E-5
7	1.411015060852242E-4	0.0	5.240913083165469E-5	4.031471602434976E-6	2.0157358012174882E-5
8	1.7738475050713896E-4	0.0	5.240913083165469E-5	4.031471602434976E-6	2.0157358012174882E-5
9	1.854476937120089E-4	0.0	5.240913083165469E-5	4.031471602434976E-6	4.0314716024349764E-5
10-11	2.0963652332661877E-4	0.0	5.644060243408967E-5	4.031471602434976E-6	4.0314716024349764E-5
12-13	2.721243331643609E-4	0.0	5.644060243408967E-5	4.031471602434976E-6	4.0314716024349764E-5
14-15	3.346121430021031E-4	0.0	5.644060243408967E-5	4.031471602434976E-6	4.233045182556725E-5
16-17	3.991156886410627E-4	0.0	5.644060243408967E-5	4.031471602434976E-6	4.434618762678474E-5
18-19	4.6966644168367475E-4	0.0	5.644060243408967E-5	4.031471602434976E-6	4.8377659229219716E-5
20-21	5.724689675457667E-4	0.0	6.0472074036524646E-5	4.031471602434976E-6	5.240913083165469E-5
22-23	6.93413115618816E-4	0.0	6.0472074036524646E-5	4.031471602434976E-6	5.240913083165469E-5
24-25	8.264516784991702E-4	0.0	6.0472074036524646E-5	4.031471602434976E-6	5.240913083165469E-5
26-27	9.776318635904818E-4	4.031471602434976E-6	6.0472074036524646E-5	4.031471602434976E-6	5.240913083165469E-5
28-29	0.0011933155943207531	4.031471602434976E-6	7.256648884382957E-5	4.031471602434976E-6	5.442486663287218E-5
30-31	0.0014876130212985064	4.031471602434976E-6	7.458222464504707E-5	4.031471602434976E-6	6.248780983774213E-5
32-33	0.0018101307494933045	4.031471602434976E-6	7.659796044626455E-5	4.031471602434976E-6	6.450354563895962E-5
34-35	0.0022011834949294973	4.031471602434976E-6	7.659796044626455E-5	4.031471602434976E-6	6.450354563895962E-5
36-37	0.0027091489168363046	4.031471602434976E-6	8.062943204869953E-5	4.031471602434976E-6	6.85350172413946E-5
38-39	0.0033239483362076383	4.031471602434976E-6	8.062943204869953E-5	4.031471602434976E-6	7.256648884382957E-5
40-41	0.004273359898581075	8.062943204869953E-6	8.062943204869953E-5	4.031471602434976E-6	7.256648884382957E-5
42-43	0.0054989272657213085	2.0157358012174882E-5	8.46609036511345E-5	8.062943204869953E-6	7.659796044626455E-5
44-45	0.007119578849900169	2.217309381339237E-5	8.46609036511345E-5	8.062943204869953E-6	8.869237525356948E-5
46-47	0.00878860809330825	2.4188829614609858E-5	8.46609036511345E-5	1.0078679006087441E-5	9.272384685600446E-5
48-49	0.01084062713894765	2.4188829614609858E-5	9.675531845843943E-5	1.2094414807304929E-5	9.272384685600446E-5
50-51	0.013664672996453353	2.4188829614609858E-5	9.675531845843943E-5	1.2094414807304929E-5	9.272384685600446E-5
52-53	0.017371611134892315	2.4188829614609858E-5	9.675531845843943E-5	1.2094414807304929E-5	1.0481826166330939E-4
54-55	0.02179211974696227	2.4188829614609858E-5	1.1086546906696184E-4	1.2094414807304929E-5	1.0884973326574436E-4
56-57	0.026591586689661106	3.628324442191479E-5	1.5722739249496407E-4	1.2094414807304929E-5	1.0884973326574436E-4
58-59	0.031651083550717	3.8298980223132276E-5	1.69321807302269E-4	1.2094414807304929E-5	1.0884973326574436E-4
60-61	0.037690228011164596	4.0314716024349764E-5	1.69321807302269E-4	1.2094414807304929E-5	1.0884973326574436E-4
62-63	0.04590636713692708	4.0314716024349764E-5	1.69321807302269E-4	1.2094414807304929E-5	1.0884973326574436E-4
64-65	0.05580161418510372	4.0314716024349764E-5	1.713375431034865E-4	1.2094414807304929E-5	1.0884973326574436E-4
66-67	0.06690227124240844	4.0314716024349764E-5	1.7738475050713896E-4	1.2094414807304929E-5	1.0884973326574436E-4
68-69	0.08017790722922682	4.233045182556725E-5	1.8141622210957395E-4	1.2094414807304929E-5	1.1288120486817934E-4
70-71	0.09581195410346965	4.434618762678474E-5	1.8141622210957395E-4	1.2094414807304929E-5	1.1288120486817934E-4
72-73	0.11508440409891006	4.434618762678474E-5	1.894791653144439E-4	1.2094414807304929E-5	1.1288120486817934E-4
74-75	0.13893660583471662	6.0472074036524646E-5	1.894791653144439E-4	1.2094414807304929E-5	1.1288120486817934E-4
76-77	0.16883803070997683	7.256648884382957E-5	1.894791653144439E-4	1.2094414807304929E-5	1.1288120486817934E-4
78-79	0.2054296827094779	7.256648884382957E-5	1.9351063691687887E-4	1.2094414807304929E-5	1.1288120486817934E-4
80-81	0.24962872162277375	7.256648884382957E-5	1.9351063691687887E-4	1.2094414807304929E-5	1.1288120486817934E-4
82-83	0.30225756765676115	7.256648884382957E-5	1.9351063691687887E-4	1.2094414807304929E-5	1.2900709127791924E-4
84-85	0.36766013146306376	8.264516784991702E-5	1.9351063691687887E-4	1.2094414807304929E-5	1.2900709127791924E-4
86-87	0.45053912466592205	9.070811105478698E-5	1.9351063691687887E-4	1.2094414807304929E-5	1.2900709127791924E-4
88-89	0.5523398298348088	9.675531845843943E-5	1.9754210851931386E-4	1.2094414807304929E-5	1.3303856288035423E-4
90	0.6415079187374656	1.0078679006087441E-4	2.056050517241838E-4	1.2094414807304929E-5	1.3303856288035423E-4
>>END_MODULE
