##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654124_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34485844
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63098046839161	32.0	32.0	32.0	32.0	32.0
2	31.611302394106986	32.0	32.0	32.0	32.0	32.0
3	35.70626814295164	37.0	37.0	37.0	32.0	37.0
4	36.33370530238437	37.0	37.0	37.0	37.0	37.0
5	36.5170715555055	37.0	37.0	37.0	37.0	37.0
6	40.08952656052147	41.0	41.0	41.0	37.0	41.0
7	40.12640746156597	41.0	41.0	41.0	37.0	41.0
8	40.2174488175496	41.0	41.0	41.0	37.0	41.0
9	40.23520474082061	41.0	41.0	41.0	41.0	41.0
10-11	40.294838195057665	41.0	41.0	41.0	41.0	41.0
12-13	40.2694241730027	41.0	41.0	41.0	41.0	41.0
14-15	40.21910758512971	41.0	41.0	41.0	41.0	41.0
16-17	40.24265458023878	41.0	41.0	41.0	41.0	41.0
18-19	40.238010689255574	41.0	41.0	41.0	41.0	41.0
20-21	40.21786368342906	41.0	41.0	41.0	41.0	41.0
22-23	40.20813880907192	41.0	41.0	41.0	41.0	41.0
24-25	40.192770111701485	41.0	41.0	41.0	41.0	41.0
26-27	40.141261150517295	41.0	41.0	41.0	41.0	41.0
28-29	40.119907577149625	41.0	41.0	41.0	39.0	41.0
30-31	40.09556012316242	41.0	41.0	41.0	37.0	41.0
32-33	40.09130289808189	41.0	41.0	41.0	37.0	41.0
34-35	40.07389953976478	41.0	41.0	41.0	37.0	41.0
36-37	39.97073380312223	41.0	41.0	41.0	37.0	41.0
38-39	40.01061483372714	41.0	41.0	41.0	37.0	41.0
40-41	40.01456805000916	41.0	41.0	41.0	37.0	41.0
42-43	40.002974887318985	41.0	41.0	41.0	37.0	41.0
44-45	39.98380636994125	41.0	41.0	41.0	37.0	41.0
46-47	39.965744944505346	41.0	41.0	41.0	37.0	41.0
48-49	39.940462150208646	41.0	41.0	41.0	37.0	41.0
50-51	39.880704906047825	41.0	41.0	41.0	37.0	41.0
52-53	39.875717250823264	41.0	41.0	41.0	37.0	41.0
54-55	39.83299108178997	41.0	41.0	41.0	37.0	41.0
56-57	39.82186426987259	41.0	41.0	41.0	37.0	41.0
58-59	39.78443379550171	41.0	41.0	41.0	37.0	41.0
60-61	39.78789969588681	41.0	41.0	41.0	37.0	41.0
62-63	39.73497155818486	41.0	41.0	41.0	37.0	41.0
64-65	39.74155892777338	41.0	41.0	41.0	37.0	41.0
66-67	39.683955277417596	41.0	41.0	41.0	37.0	41.0
68-69	39.64920364135499	41.0	41.0	41.0	37.0	41.0
70-71	39.648008788765615	41.0	41.0	41.0	37.0	41.0
72-73	39.62626830301732	41.0	41.0	41.0	37.0	41.0
74-75	39.57998123809874	41.0	41.0	41.0	37.0	41.0
76-77	39.01384157801097	41.0	39.0	41.0	34.5	41.0
78-79	39.38340672479989	41.0	41.0	41.0	37.0	41.0
80-81	39.671889094551375	41.0	41.0	41.0	37.0	41.0
82-83	39.695001346059556	41.0	41.0	41.0	37.0	41.0
84-85	39.7092247184091	41.0	41.0	41.0	37.0	41.0
86-87	39.6906120087999	41.0	41.0	41.0	37.0	41.0
88-89	39.64829935726671	41.0	41.0	41.0	37.0	41.0
90-91	39.61193939461073	41.0	41.0	41.0	37.0	41.0
92-93	39.56367863579038	41.0	41.0	41.0	37.0	41.0
94-95	39.540597034539736	41.0	41.0	41.0	37.0	41.0
96-97	39.52018690625637	41.0	41.0	41.0	37.0	41.0
98-99	39.4755366288846	41.0	41.0	41.0	37.0	41.0
100-101	38.94721486300291	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	5.0
17	22.0
18	62.0
19	210.0
20	632.0
21	1828.0
22	4584.0
23	9928.0
24	18647.0
25	31842.0
26	52551.0
27	81438.0
28	117085.0
29	161137.0
30	211417.0
31	265613.0
32	327823.0
33	398888.0
34	479560.0
35	582438.0
36	727200.0
37	962004.0
38	1439427.0
39	3228385.0
40	2.5383117E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.67186733241082	11.167976201528946	6.498549532682306	49.66160693337793
2	22.584405937694317	13.420384897640899	33.68120264071252	30.314006523952266
3	20.67311324044363	14.563027938101175	20.49188654134546	44.271972280109736
4	23.82769869283176	22.122369978823777	20.137999232380682	33.911932095963785
5	24.90884375629606	27.224950620318296	23.671211294698196	24.194994328687446
6	20.498022318954987	31.642844524843294	25.04391077103985	22.815222385161864
7	15.95850169710215	25.813629499686886	39.63760318581734	18.590265617393616
8	18.375350349618877	24.76457097163473	32.324461560017944	24.535617118728446
9	18.451689916593022	22.543296316018825	35.072332867944304	23.93268089944384
10-11	20.76678622103609	31.615879837535655	25.5825665742732	22.034767367155055
12-13	21.450972752761974	25.54104518944063	27.76959583764283	25.238386220154563
14-15	21.01204369561931	26.52794128278276	27.34084368766223	25.119171333935704
16-17	21.63643725813989	26.754401023214047	26.738433601915034	24.870728116731026
18-19	21.370584695563778	26.9324146452672	26.548032288262974	25.148968370906044
20-21	21.66412398084269	26.86290496471538	26.76419779663795	24.708773257803983
22-23	21.480803833596184	27.04483758611215	26.7587897805256	24.71556879976607
24-25	21.41694237301209	26.726600748455915	26.696043124005293	25.1604137545267
26-27	21.356992161769334	26.88328434125028	26.777378567275314	24.982344929705068
28-29	21.310720379297678	27.04899575349229	26.609548965342277	25.030734901867753
30-31	21.445257364152084	26.75866944129307	26.517351583449724	25.278721611105126
32-33	21.56039582565476	26.852478101917743	26.603677431138077	24.983448641289417
34-35	21.79544456836333	26.903585969433642	26.35524031746265	24.945729144740383
36-37	21.539646818561263	26.71423091747443	26.385648267735597	25.36047399622871
38-39	21.527240278648826	26.80745322631512	26.652794114593803	25.01251238044225
40-41	21.717696687371202	27.193832054683075	26.25970963622059	24.828761621725135
42-43	21.685047058729374	26.809177122067823	26.408564917245464	25.097210901957332
44-45	21.731989508506736	26.89560243907616	26.505433649818748	24.866974402598352
46-47	21.679486806238522	26.798958436394944	26.511853675380543	25.009701081985984
48-49	21.582407842475888	26.5946064709914	26.42500934586377	25.397976340668944
50-51	21.65444174717023	26.565394484763083	26.67460741282713	25.10555635523956
52-53	22.016707783054404	26.874635865081338	26.136770786297127	24.971885565567135
54-55	21.883673486431128	26.71229969027291	26.203653301917157	25.2003735213788
56-57	21.7525080725877	26.65441651942751	26.62538286724257	24.967692540742227
58-59	21.776884741460872	26.665373189068536	26.55432762498143	25.003414444489163
60-61	21.8134693180193	26.379840087428335	26.596649338203814	25.210041256348546
62-63	21.786329196408822	26.468992610417192	26.53855013668797	25.206128056486016
64-65	21.84156925374945	26.711351475115414	26.319922748592145	25.127156522542986
66-67	21.833744303894665	26.477832469461966	26.302559102221768	25.3858641244216
68-69	21.868799267316756	26.57491868257596	26.647225162881327	24.909056887225958
70-71	22.098247037248093	26.858362521155055	26.347287600094692	24.696102841502153
72-73	22.168406724245482	26.36519977247468	26.241834617764187	25.224558885515652
74-75	22.081722095752568	26.409576677606967	26.53401827513498	24.97468295150549
76-77	21.945471307009335	26.62278759945675	26.515128932323652	24.916612161210264
78-79	21.83670648607446	26.47270674119751	26.39485332302054	25.29573344970749
80-81	21.98941832480597	26.500533088301392	26.515591440940227	24.99445714595241
82-83	22.209511531746184	26.767912364273293	26.152187256893	24.87038884708752
84-85	22.16047691916718	26.523642570557353	26.14885690487958	25.167023605395883
86-87	22.084485582991096	26.49114992083646	26.423667459164758	25.000697037007686
88-89	22.264450514054584	26.478041765093565	26.320878672725655	24.9366290481262
90-91	22.254983813068343	26.290683214828668	26.32711265526806	25.12722031683493
92-93	22.22210832645464	26.2344166667005	26.4901340908565	25.053340915988358
94-95	22.364107088361898	26.362137728417512	26.339103286743843	24.93465189647675
96-97	22.358762627355155	26.241890730584988	26.222682849229383	25.17666379283047
98-99	22.308978431845834	26.514225663144565	26.342072416728442	24.834723488281163
100-101	22.46201948834426	26.665232551652206	26.10716298548471	24.76558497451882
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	62.0
1	48.5
2	29.5
3	30.5
4	50.5
5	67.0
6	85.5
7	115.0
8	157.5
9	193.5
10	220.5
11	256.0
12	316.0
13	367.0
14	414.5
15	493.0
16	576.0
17	705.5
18	926.0
19	1160.5
20	1543.0
21	2134.5
22	3146.5
23	4643.5
24	6722.0
25	9934.0
26	14876.5
27	21983.5
28	31102.0
29	44349.5
30	63001.0
31	86767.0
32	122412.5
33	165895.0
34	212276.5
35	277403.0
36	367897.0
37	484027.0
38	630483.0
39	833445.5
40	1072320.5
41	1313160.0
42	1550365.5
43	1774703.5
44	1982978.0
45	2131854.5
46	2214957.5
47	2222690.5
48	2129392.5
49	1995708.0
50	1845312.0
51	1656635.0
52	1482961.5
53	1299832.0
54	1109546.0
55	960780.0
56	814892.0
57	669135.0
58	539721.5
59	422755.5
60	332210.5
61	290809.5
62	259227.5
63	215642.0
64	169937.5
65	135115.5
66	117818.0
67	97270.5
68	72709.5
69	54945.0
70	42358.5
71	31195.5
72	24003.5
73	20995.0
74	17687.0
75	12640.0
76	7513.0
77	2772.5
78	644.0
79	217.0
80	75.5
81	32.0
82	12.0
83	5.5
84	4.5
85	3.0
86	1.5
87	1.5
88	1.5
89	0.5
90	0.0
91	0.0
92	1.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02416933742436462
2	0.0
3	0.002490877126278249
4	0.0
5	0.0
6	0.0
7	0.0
8	8.467242385020358E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0024096843910794237
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.190535571639193E-4
26-27	0.0
28-29	6.161948653482281E-4
30-31	0.0
32-33	1.304883244266836E-5
34-35	6.66940324847494E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	3.4796886513782295E-5
74-75	5.480509625920712E-4
76-77	0.0
78-79	0.0015209139147065677
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0017035975689039249
88-89	3.4796886513782295E-5
90-91	0.0
92-93	3.537683462234533E-4
94-95	0.001531063006606421
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4485844E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.11714062148794
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.88579720917229	16.78165865394011
2	15.501738059237416	8.40725621659625
3	6.7591474847191355	5.498662584735159
4	3.7033061986145492	4.016922998090347
5	2.3641624215864776	3.2054662419098983
6	1.6257414222319153	2.645127525650439
7	1.194187252725999	2.266806056239149
8	0.9182819547425549	1.9920944717542952
9	0.7159615658648637	1.7473347415024387
>10	4.653814573317449	23.853539544499068
>50	0.3988726929935062	7.48723558998581
>100	0.24737194325530198	12.705292602358446
>500	0.02054102619842921	3.7921466063836395
>1k	0.0107844126502551	5.066913735729547
>5k	2.58115456824299E-4	0.43187530467891533
>10k+	3.3667233498821604E-5	0.10166712594661031
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.609766488533672E-5	0.0	0.0	0.0	2.899740542815191E-6
2	4.3496108142227864E-5	0.0	0.0	0.0	2.899740542815191E-6
3	7.829299465601016E-5	0.0	0.0	0.0	5.799481085630382E-6
4	1.333880649694988E-4	0.0	8.699221628445574E-6	0.0	5.799481085630382E-6
5	1.652852109404659E-4	0.0	8.699221628445574E-6	0.0	5.799481085630382E-6
6	2.1168105962550895E-4	0.0	2.899740542815191E-5	0.0	5.799481085630382E-6
7	2.3487898396803048E-4	0.0	3.4796886513782295E-5	0.0	5.799481085630382E-6
8	2.667761299389976E-4	0.0	3.4796886513782295E-5	0.0	5.799481085630382E-6
9	2.8417457319588875E-4	0.0	3.4796886513782295E-5	0.0	5.799481085630382E-6
10-11	3.11722108352633E-4	8.699221628445574E-6	4.059636759941268E-5	0.0	5.799481085630382E-6
12-13	3.7841614083738246E-4	1.4498702714075955E-5	4.3496108142227864E-5	2.899740542815191E-6	1.014909189985317E-5
14-15	4.3786082196509385E-4	1.4498702714075955E-5	4.3496108142227864E-5	2.899740542815191E-6	1.1598962171260765E-5
16-17	4.871564111929521E-4	2.319792434252153E-5	4.3496108142227864E-5	2.899740542815191E-6	1.304883244266836E-5
18-19	5.379018706922179E-4	2.319792434252153E-5	4.3496108142227864E-5	2.899740542815191E-6	1.4498702714075955E-5
20-21	5.857475896486686E-4	2.319792434252153E-5	4.3496108142227864E-5	2.899740542815191E-6	2.029818379970634E-5
22-23	6.480920113191952E-4	2.319792434252153E-5	4.3496108142227864E-5	2.899740542815191E-6	2.029818379970634E-5
24-25	7.582821519461724E-4	2.319792434252153E-5	4.3496108142227864E-5	2.899740542815191E-6	2.029818379970634E-5
26-27	8.786213844730029E-4	2.609766488533672E-5	4.3496108142227864E-5	2.899740542815191E-6	2.029818379970634E-5
28-29	0.001054055687313322	2.609766488533672E-5	4.639584868504306E-5	2.899740542815191E-6	2.029818379970634E-5
30-31	0.0012439886928677169	2.609766488533672E-5	4.639584868504306E-5	2.899740542815191E-6	2.319792434252153E-5
32-33	0.001523813655249383	2.609766488533672E-5	4.639584868504306E-5	2.899740542815191E-6	2.609766488533672E-5
34-35	0.0019196282393436565	2.899740542815191E-5	4.784571895645065E-5	2.899740542815191E-6	2.899740542815191E-5
36-37	0.0025010262181781023	2.899740542815191E-5	4.9295589227858245E-5	2.899740542815191E-6	2.899740542815191E-5
38-39	0.003326002402609024	2.899740542815191E-5	4.9295589227858245E-5	2.899740542815191E-6	3.33470162423747E-5
40-41	0.004382957830465162	2.899740542815191E-5	4.9295589227858245E-5	4.349610814222787E-6	4.3496108142227864E-5
42-43	0.0057733834207450456	5.074545949926584E-5	4.9295589227858245E-5	5.799481085630382E-6	4.3496108142227864E-5
44-45	0.007397238124721553	7.394338384178737E-5	4.9295589227858245E-5	5.799481085630382E-6	4.3496108142227864E-5
46-47	0.009221174926152308	9.134182709867852E-5	4.9295589227858245E-5	5.799481085630382E-6	4.494597841363546E-5
48-49	0.011658406852388475	9.569143791290131E-5	4.9295589227858245E-5	5.799481085630382E-6	4.9295589227858245E-5
50-51	0.015067051860467733	1.0004104872712409E-4	4.9295589227858245E-5	5.799481085630382E-6	5.5095070313488634E-5
52-53	0.01923977850157879	1.0439065954134688E-4	4.9295589227858245E-5	5.799481085630382E-6	5.799481085630382E-5
54-55	0.024079445467537347	1.0584052981275448E-4	4.9295589227858245E-5	5.799481085630382E-6	5.944468112771142E-5
56-57	0.029484561839344864	1.275885838838684E-4	5.074545949926584E-5	5.799481085630382E-6	6.66940324847494E-5
58-59	0.036043774947192825	1.333880649694988E-4	7.249351357037977E-5	5.799481085630382E-6	6.8143902756157E-5
60-61	0.044653104618811126	1.3483793524090638E-4	7.249351357037977E-5	5.799481085630382E-6	6.959377302756459E-5
62-63	0.055206710324387016	1.36287805512314E-4	7.249351357037977E-5	5.799481085630382E-6	6.959377302756459E-5
64-65	0.06768139413957797	1.36287805512314E-4	7.249351357037977E-5	5.799481085630382E-6	7.539325411319497E-5
66-67	0.08318485695173938	1.36287805512314E-4	7.249351357037977E-5	5.799481085630382E-6	7.539325411319497E-5
68-69	0.1014735205552748	1.36287805512314E-4	7.684312438460256E-5	5.799481085630382E-6	8.264260547023294E-5
70-71	0.1236753840213393	1.377376757837216E-4	7.829299465601016E-5	5.799481085630382E-6	9.569143791290131E-5
72-73	0.15117652332939857	1.4208728659794438E-4	8.119273519882535E-5	5.799481085630382E-6	9.714130818430891E-5
74-75	0.18647796469763073	1.4788676768357474E-4	8.409247574164055E-5	5.799481085630382E-6	9.859117845571649E-5
76-77	0.2304742780834942	1.609356001262431E-4	8.409247574164055E-5	5.799481085630382E-6	1.0004104872712409E-4
78-79	0.28397739083897733	1.623854703976507E-4	8.409247574164055E-5	5.799481085630382E-6	1.0439065954134688E-4
80-81	0.34848501895444406	1.652852109404659E-4	8.409247574164055E-5	5.799481085630382E-6	1.0439065954134688E-4
82-83	0.42795965788165136	1.652852109404659E-4	8.409247574164055E-5	5.799481085630382E-6	1.0439065954134688E-4
84-85	0.5269857974187901	1.6963482175468868E-4	8.409247574164055E-5	5.799481085630382E-6	1.0584052981275448E-4
86-87	0.6492069615579077	1.7398443256891146E-4	8.409247574164055E-5	5.799481085630382E-6	1.0729040008416207E-4
88-89	0.7983000213072935	1.7978391365454184E-4	8.409247574164055E-5	5.799481085630382E-6	1.1164001089838485E-4
90	0.9243676912764553	1.7978391365454184E-4	8.989195682727092E-5	5.799481085630382E-6	1.2178910279823803E-4
>>END_MODULE
