##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654115_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30751883
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23447725136051	32.0	32.0	32.0	32.0	32.0
2	31.260424638062002	32.0	32.0	32.0	32.0	32.0
3	34.93264805930746	37.0	32.0	37.0	32.0	37.0
4	35.707148762240024	37.0	37.0	37.0	32.0	37.0
5	35.96610035229387	37.0	37.0	37.0	37.0	37.0
6	39.35001092453428	41.0	41.0	41.0	37.0	41.0
7	39.3497508428996	41.0	41.0	41.0	37.0	41.0
8	39.46244221857894	41.0	41.0	41.0	37.0	41.0
9	39.51697465160101	41.0	41.0	41.0	37.0	41.0
10-11	39.542841490389385	41.0	41.0	41.0	37.0	41.0
12-13	39.534543169275196	41.0	41.0	41.0	37.0	41.0
14-15	39.42682984648452	41.0	41.0	41.0	37.0	41.0
16-17	39.39262670516794	41.0	41.0	41.0	37.0	41.0
18-19	39.18976823305422	41.0	41.0	41.0	37.0	41.0
20-21	39.07973573520685	41.0	41.0	41.0	37.0	41.0
22-23	38.877757957130626	41.0	41.0	41.0	37.0	41.0
24-25	38.88798799735288	41.0	41.0	41.0	37.0	41.0
26-27	38.90191855893832	41.0	41.0	41.0	37.0	41.0
28-29	39.071906247171924	41.0	41.0	41.0	37.0	41.0
30-31	39.08538994181267	41.0	41.0	41.0	37.0	41.0
32-33	39.149252909163316	41.0	41.0	41.0	37.0	41.0
34-35	39.170797378488984	41.0	41.0	41.0	37.0	41.0
36-37	39.19112133718771	41.0	41.0	41.0	37.0	41.0
38-39	39.17970985711672	41.0	41.0	41.0	37.0	41.0
40-41	39.12170345796386	41.0	41.0	41.0	37.0	41.0
42-43	39.11503708569651	41.0	41.0	41.0	37.0	41.0
44-45	39.11247717741382	41.0	41.0	41.0	37.0	41.0
46-47	39.06146888631177	41.0	41.0	41.0	37.0	41.0
48-49	39.01067061486934	41.0	41.0	41.0	37.0	41.0
50-51	39.047986020888544	41.0	41.0	41.0	37.0	41.0
52-53	39.03551762992855	41.0	41.0	41.0	37.0	41.0
54-55	39.01708147432793	41.0	41.0	41.0	37.0	41.0
56-57	38.97129187178554	41.0	41.0	41.0	37.0	41.0
58-59	38.87269729141464	41.0	41.0	41.0	32.0	41.0
60-61	38.90169244270343	41.0	41.0	41.0	32.0	41.0
62-63	38.88529778810618	41.0	41.0	41.0	32.0	41.0
64-65	38.85379509931148	41.0	41.0	41.0	32.0	41.0
66-67	38.8078575708681	41.0	41.0	41.0	32.0	41.0
68-69	38.77618482744617	41.0	41.0	41.0	32.0	41.0
70-71	38.73847983552747	41.0	41.0	41.0	32.0	41.0
72-73	38.708746729427915	41.0	41.0	41.0	32.0	41.0
74-75	38.68380082611527	41.0	41.0	41.0	32.0	41.0
76-77	37.943368248376856	41.0	39.0	41.0	32.0	41.0
78-79	38.388753235045805	41.0	39.0	41.0	32.0	41.0
80-81	38.6978623878089	41.0	41.0	41.0	32.0	41.0
82-83	38.849412928632695	41.0	41.0	41.0	32.0	41.0
84-85	38.79093273735465	41.0	41.0	41.0	32.0	41.0
86-87	38.72816669470289	41.0	41.0	41.0	32.0	41.0
88-89	38.663105426747364	41.0	41.0	41.0	32.0	41.0
90-91	38.598403567677465	41.0	41.0	41.0	32.0	41.0
92-93	38.519591499486395	41.0	39.0	41.0	32.0	41.0
94-95	38.50516804125458	41.0	37.0	41.0	32.0	41.0
96-97	38.3931065131849	41.0	37.0	41.0	32.0	41.0
98-99	38.177356082552734	41.0	37.0	41.0	32.0	41.0
100-101	37.20623488324276	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	22.0
13	185.0
14	747.0
15	1674.0
16	5461.0
17	13964.0
18	25200.0
19	36138.0
20	46733.0
21	59416.0
22	73976.0
23	90846.0
24	109863.0
25	131356.0
26	153416.0
27	180965.0
28	210831.0
29	245527.0
30	289085.0
31	341695.0
32	400980.0
33	477741.0
34	582309.0
35	725976.0
36	945908.0
37	1317774.0
38	2149101.0
39	5178637.0
40	1.6956357E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	34.68731375524088	21.73364429430641	11.06261012026369	32.51643183018903
2	29.648392587862016	25.62708761606566	28.424509809691976	16.300009986380346
3	21.413335391098457	27.764158524787923	28.677766566513206	22.144739517600414
4	25.00032599629753	30.872011967527325	23.19866396473998	20.92899807143517
5	27.548904454939393	32.37363321303504	21.673540459996662	18.403921872028906
6	22.85096818298899	36.8969796093462	21.44590625556165	18.806145952103158
7	22.171087864765877	22.3614924653557	35.1932400367158	20.274179633162625
8	23.20956085843589	24.932769157582968	26.55023433849563	25.307435645485516
9	23.755182731411928	23.22757926725983	29.343864894387117	23.673373106941124
10-11	25.215333992492116	29.927505269891086	23.282313537102183	21.57484720051461
12-13	25.71783041244921	25.007401201921375	25.970009458319822	23.304758927309596
14-15	24.972573224215246	26.49047864808799	26.070883204127693	22.46606492356907
16-17	25.744239922018913	26.18162751003335	25.461387591136237	22.6127449768115
18-19	25.41334670010484	26.814804175296153	25.38121532965864	22.390633794940364
20-21	25.54308283979066	26.72618369757707	25.307770572848565	22.422962889783705
22-23	25.34310288175349	26.567415498414555	25.44930335743056	22.6401782624014
24-25	25.164229634570873	26.521301081717773	25.69828711569905	22.61618216801231
26-27	25.414229105905484	26.62343473406165	25.56929278119327	22.393043378839597
28-29	25.370470634264976	26.38509909343054	25.640934379380564	22.60349589292392
30-31	25.068378739604334	26.356293369092228	25.846718069264245	22.72860982203919
32-33	25.271688676146546	26.52168625748665	25.61164087757339	22.594984188793422
34-35	25.287690513130528	26.614848267990617	25.416295646025965	22.681165572852887
36-37	24.98636912737994	26.380921129284996	26.02642576391176	22.60628397942331
38-39	25.36290062559211	26.55664966688928	25.72067705874248	22.359772648776126
40-41	25.395074181311113	26.36048173050086	25.484543499336283	22.759900588851746
42-43	24.968820361087097	26.357663407794295	26.043070419758763	22.63044581135984
44-45	25.17568648702876	26.46561359890991	25.988938943223488	22.36976097083784
46-47	25.38614106919586	26.17802719883468	25.690037891294086	22.74579384067537
48-49	24.976657643096146	26.3065277091113	26.073400873560626	22.643413774231927
50-51	25.35014197341997	26.234466682901985	25.76532955721768	22.65006178646036
52-53	25.467049076607207	26.196251080942424	25.60870651645686	22.727993325993502
54-55	24.860309839269632	26.278955759822008	26.14825737299852	22.712477027909838
56-57	24.99620579852844	26.372765181187873	25.891108905500595	22.73992011478309
58-59	25.290626918683323	26.10267963103268	25.65596877433489	22.95072467594911
60-61	25.140104501622616	26.26750596963755	25.89328478820489	22.69910474053494
62-63	25.06596878804156	26.156317150460545	26.15042951905222	22.62728454244568
64-65	25.224798429416502	26.137802683497462	25.89270224525763	22.744696641828405
66-67	25.05902413186012	26.25745729186088	26.08980363640195	22.59371493987705
68-69	25.176473384825606	26.530252351465002	25.814573997917872	22.47870026579152
70-71	25.519759555496098	26.19793486049223	25.56847171962866	22.71383386438302
72-73	24.893978037052236	26.34803533819376	26.091194155492854	22.66679246926115
74-75	24.92851106390193	26.440959779686295	25.938319063186782	22.69221009322499
76-77	25.524255563473673	26.15682523424887	25.547536226812912	22.77138297546454
78-79	25.00317297643204	26.45775057091626	25.78274962869753	22.75632682395416
80-81	25.182721043748597	26.40375828842921	25.742893657705352	22.670627010116842
82-83	25.41654822374807	26.152113502674823	25.670399655769096	22.76093861780801
84-85	25.16743963675815	26.403140871595998	25.9538233483947	22.475596143251156
86-87	25.17209433971897	26.70680361264382	25.558181266493502	22.56292078114371
88-89	25.35744438033758	26.235375863745634	25.697984357075377	22.70919539884141
90-91	24.983268090416423	26.570995951969685	25.9415870635117	22.504148894102194
92-93	25.2419576410032	26.590566324205046	25.6790658566059	22.48841017818586
94-95	25.644109337955012	26.362891339044182	25.47021592141203	22.522783401588775
96-97	25.414679573152917	26.360733530869556	25.910329280986634	22.314257614990893
98-99	25.53729605615137	26.63527129359825	25.49813421960984	22.329298430640534
100-101	25.683255389752023	26.442587657201255	25.427477024099232	22.446679928947493
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	29.5
2	24.5
3	29.5
4	41.5
5	57.0
6	64.0
7	74.0
8	95.5
9	122.0
10	132.5
11	150.5
12	198.0
13	248.0
14	300.5
15	384.5
16	462.5
17	559.5
18	690.0
19	834.5
20	1116.0
21	1425.0
22	1855.0
23	2437.5
24	3117.0
25	4151.5
26	5653.0
27	7538.5
28	10379.0
29	14639.0
30	20707.5
31	29720.5
32	43828.5
33	64992.5
34	99110.5
35	153436.0
36	233556.5
37	349464.0
38	509407.5
39	715863.5
40	949324.5
41	1176652.0
42	1396712.5
43	1590038.0
44	1756973.5
45	1937788.5
46	2004141.0
47	1968637.5
48	1913077.0
49	1800649.0
50	1660744.5
51	1553759.0
52	1407191.5
53	1193858.0
54	1012419.0
55	864962.0
56	728789.0
57	602484.0
58	501308.0
59	404904.5
60	344868.5
61	314279.0
62	279996.5
63	232055.5
64	180530.5
65	148176.5
66	128900.0
67	111533.0
68	85428.5
69	60273.5
70	44852.0
71	37597.0
72	30022.0
73	20744.0
74	13399.0
75	7641.0
76	3982.0
77	1817.5
78	936.5
79	551.0
80	357.5
81	270.0
82	200.5
83	157.0
84	138.5
85	109.0
86	87.5
87	85.0
88	69.0
89	55.0
90	50.0
91	40.5
92	35.0
93	36.0
94	42.5
95	42.0
96	40.5
97	44.0
98	58.5
99	116.5
100	509.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04150314957949079
2	0.0
3	2.4063567099289498E-4
4	0.0
5	0.008084057812004553
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.999877535954465E-4
12-13	3.9347184040730126E-4
14-15	0.0
16-17	0.0010552199356377624
18-19	1.4633250263081451E-5
20-21	0.0013169925236773306
22-23	4.471270913719332E-4
24-25	0.002881124385131148
26-27	0.0
28-29	0.0020372736199601176
30-31	0.0
32-33	0.004498911497549597
34-35	0.0
36-37	0.0
38-39	0.0022632760406899313
40-41	0.0
42-43	7.934473475981943E-4
44-45	9.771759342346615E-4
46-47	0.001599902028763572
48-49	0.0012438262723619233
50-51	0.0
52-53	0.0030534715548963293
54-55	5.34926592950422E-4
56-57	0.0032908553924974287
58-59	0.0
60-61	5.202933426873404E-5
62-63	0.003177041223784573
64-65	0.0
66-67	0.0022421391236432577
68-69	4.861490920734838E-4
70-71	0.00272178454893315
72-73	0.0
74-75	3.138019223083022E-4
76-77	0.003385158560859509
78-79	0.0
80-81	0.003846918902494524
82-83	0.0014031661085599212
84-85	0.0026047185468284985
86-87	0.0
88-89	9.121392663987438E-4
90-91	4.389975078924435E-5
92-93	3.7721267344832183E-4
94-95	0.0
96-97	0.002373838376010991
98-99	1.788508365487733E-5
100-101	8.438507651710304E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.0751883E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.01681600981519
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.99595451766075	17.289669285761416
2	14.0557191998926	7.594815590182503
3	6.484453040130248	5.25567824128457
4	3.669145880782675	3.9651455669710733
5	2.375761921700674	3.2092761360856037
6	1.6253196792247486	2.634657763844813
7	1.1396013282465545	2.1551879586844715
8	0.8620098415769039	1.8631009030826522
9	0.7197503674652391	1.7500826925724717
>10	4.38802947748155	22.294000885782438
>50	0.3890344200694945	7.286055045012859
>100	0.260060722449408	13.650196175374807
>500	0.022822217124409495	4.195822963466505
>1k	0.011812814197172566	5.678821640182455
>5k	4.496331406837762E-4	0.805733765360837
>10k+	7.493885678062937E-5	0.3717553863505992
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGAAAATCTATCATTTCCAGTCCTGGTTAGCCAACATGACACTTTCAATT	37189	0.1209324320074969	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2762833742571147E-5	9.755500175387635E-6	9.755500175387635E-6	6.503666783591756E-6	0.0
2	3.251833391795878E-5	5.5281167660529924E-5	1.3007333567183513E-5	6.503666783591756E-6	0.0
3	4.877750087693817E-5	5.5281167660529924E-5	1.3007333567183513E-5	6.503666783591756E-6	3.251833391795878E-6
4	1.1706600210465161E-4	7.154033461950932E-5	2.2762833742571147E-5	6.503666783591756E-6	3.251833391795878E-6
5	1.495843360226104E-4	8.454766818669283E-5	2.9266500526162903E-5	9.755500175387635E-6	3.251833391795878E-6
6	1.6584350298158977E-4	8.454766818669283E-5	3.902200070155054E-5	9.755500175387635E-6	3.251833391795878E-6
7	1.6909533637338565E-4	9.105133497028459E-5	3.902200070155054E-5	9.755500175387635E-6	3.251833391795878E-6
8	1.9511000350775268E-4	9.105133497028459E-5	5.202933426873405E-5	9.755500175387635E-6	6.503666783591756E-6
9	2.0161367029134444E-4	1.0080683514567222E-4	5.202933426873405E-5	9.755500175387635E-6	6.503666783591756E-6
10-11	2.6177258803956817E-4	1.0731050192926397E-4	6.991441792361138E-5	9.755500175387635E-6	1.1381416871285572E-5
12-13	3.235574224836899E-4	1.1218825201695779E-4	7.804400140310106E-5	9.755500175387635E-6	2.1136917046673207E-5
14-15	4.0485325727858683E-4	1.1869191880054955E-4	9.918091844977428E-5	9.755500175387635E-6	2.6014667134367025E-5
16-17	4.861490920734838E-4	1.2194375219234543E-4	1.2682150228003923E-4	9.755500175387635E-6	2.6014667134367025E-5
18-19	5.918336773068498E-4	1.4308066923901863E-4	1.5283616941440628E-4	9.755500175387635E-6	2.6014667134367025E-5
20-21	6.763813454935426E-4	1.59339836197998E-4	1.7722491985287534E-4	9.755500175387635E-6	2.6014667134367025E-5
22-23	7.950732642940921E-4	1.6909533637338565E-4	1.918581701159568E-4	1.1381416871285574E-5	2.6014667134367025E-5
24-25	0.0010893641862516192	1.918581701159568E-4	2.0649142037903825E-4	1.3007333567183513E-5	2.6014667134367025E-5
26-27	0.0014486917760450637	2.162469205544259E-4	2.211246706421197E-4	1.3007333567183513E-5	2.6014667134367025E-5
28-29	0.00186167461680314	2.3900975429699703E-4	2.455134210805888E-4	1.3007333567183513E-5	2.6014667134367025E-5
30-31	0.0024713933777648672	2.9429092195752693E-4	2.5689483795187436E-4	1.4633250263081451E-5	2.6014667134367025E-5
32-33	0.003193300390743552	3.251833391795878E-4	2.601466713436702E-4	1.625916695897939E-5	2.6014667134367025E-5
34-35	0.0039786181548622564	3.284351725713837E-4	3.18679672395996E-4	1.625916695897939E-5	2.9266500526162903E-5
36-37	0.004999693839886162	3.381906727467713E-4	3.430684228344651E-4	1.625916695897939E-5	3.73960840056526E-5
38-39	0.006373593447919921	3.5444983970575074E-4	3.6420533988113836E-4	1.625916695897939E-5	3.902200070155054E-5
40-41	0.00817185731358304	3.5932758979344455E-4	3.73960840056526E-4	1.625916695897939E-5	3.902200070155054E-5
42-43	0.010448140687840156	3.674571732729342E-4	3.98349590494995E-4	1.951100035077527E-5	3.902200070155054E-5
44-45	0.013038225984405573	3.820904235360157E-4	4.0485325727858683E-4	1.951100035077527E-5	3.902200070155054E-5
46-47	0.015979509287284945	3.9022000701550535E-4	4.52004841459627E-4	1.951100035077527E-5	3.902200070155054E-5
48-49	0.019613433102616838	4.129828407580765E-4	4.8127134198578997E-4	2.2762833742571147E-5	3.902200070155054E-5
50-51	0.024582234525280938	4.568825915473208E-4	4.926527588570756E-4	2.2762833742571147E-5	3.902200070155054E-5
52-53	0.030438786463905316	4.7476767520219815E-4	5.251710927750342E-4	2.2762833742571147E-5	3.902200070155054E-5
54-55	0.036970093831327336	4.861490920734838E-4	5.398043430381158E-4	2.6014667134367025E-5	3.902200070155054E-5
56-57	0.044717586887281016	4.991564256406673E-4	5.755745103478704E-4	2.6014667134367025E-5	3.902200070155054E-5
58-59	0.05384548321805205	5.040341757283611E-4	6.032150941781354E-4	2.6014667134367025E-5	3.902200070155054E-5
60-61	0.06511146000392887	5.07286009120157E-4	6.471148449673798E-4	2.6014667134367025E-5	3.902200070155054E-5
62-63	0.07935286434329891	5.202933426873405E-4	7.251588463704808E-4	2.6014667134367025E-5	3.902200070155054E-5
64-65	0.0966396106540858	5.365525096463199E-4	7.755622639433169E-4	2.6014667134367025E-5	3.902200070155054E-5
66-67	0.11663676009693455	5.528116766052992E-4	7.853177641187045E-4	2.6014667134367025E-5	3.902200070155054E-5
68-69	0.14029059618885778	5.723226769560745E-4	7.966991809899901E-4	2.6014667134367025E-5	3.902200070155054E-5
70-71	0.16816856385672385	5.934595940027478E-4	7.99951014381786E-4	2.6014667134367025E-5	4.5525667485142295E-5
72-73	0.20256645747514063	6.113446776576251E-4	8.162101813407654E-4	2.6014667134367025E-5	5.5281167660529924E-5
74-75	0.24591339658777966	6.162224277453188E-4	8.227138481243572E-4	2.6014667134367025E-5	5.8533001052325805E-5
76-77	0.2983443322804005	6.324815947042982E-4	8.27591598212051E-4	2.6014667134367025E-5	5.8533001052325805E-5
78-79	0.3611778829933764	6.601221785345632E-4	8.357211816915407E-4	2.6014667134367025E-5	5.8533001052325805E-5
80-81	0.43518473324056284	6.828850122771344E-4	8.666135989136015E-4	2.7640583830264962E-5	5.8533001052325805E-5
82-83	0.5245434889304177	6.9426642914842E-4	8.81246849176683E-4	2.9266500526162903E-5	5.8533001052325805E-5
84-85	0.6346668267435851	7.056478460197055E-4	8.844986825684788E-4	2.9266500526162903E-5	5.8533001052325805E-5
86-87	0.7700422767607433	7.18655179586889E-4	8.926282660479685E-4	3.414425061385672E-5	6.0158917748223746E-5
88-89	0.9308015382342603	7.397920966335622E-4	9.007578495274582E-4	3.577016730975466E-5	6.178483444412169E-5
90	1.0678663156984565	7.47921680113052E-4	9.072615163110499E-4	3.577016730975466E-5	6.503666783591755E-5
>>END_MODULE
