##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654134_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33930019
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.424968167568665	32.0	32.0	32.0	32.0	32.0
2	31.39480125254277	32.0	32.0	32.0	32.0	32.0
3	34.95448699866628	37.0	32.0	37.0	32.0	37.0
4	35.842466607519434	37.0	37.0	37.0	32.0	37.0
5	36.1283648264388	37.0	37.0	37.0	37.0	37.0
6	38.98271277124837	41.0	37.0	41.0	37.0	41.0
7	39.361251521845595	41.0	41.0	41.0	37.0	41.0
8	39.54140809647056	41.0	41.0	41.0	37.0	41.0
9	39.7643768192408	41.0	41.0	41.0	37.0	41.0
10-11	39.76132956188442	41.0	41.0	41.0	37.0	41.0
12-13	39.694008379423536	41.0	41.0	41.0	37.0	41.0
14-15	39.67634584878953	41.0	41.0	41.0	37.0	41.0
16-17	39.62348603459373	41.0	41.0	41.0	37.0	41.0
18-19	39.677596511219164	41.0	41.0	41.0	37.0	41.0
20-21	39.550280195834844	41.0	41.0	41.0	37.0	41.0
22-23	39.49427162124489	41.0	41.0	41.0	37.0	41.0
24-25	39.50569874423	41.0	41.0	41.0	37.0	41.0
26-27	39.2626166227611	41.0	41.0	41.0	37.0	41.0
28-29	39.40419967050416	41.0	41.0	41.0	37.0	41.0
30-31	39.504624710053946	41.0	41.0	41.0	37.0	41.0
32-33	39.49226176383809	41.0	41.0	41.0	37.0	41.0
34-35	39.240758456398154	41.0	41.0	41.0	37.0	41.0
36-37	39.112642406713654	41.0	41.0	41.0	37.0	41.0
38-39	39.17767424474475	41.0	41.0	41.0	37.0	41.0
40-41	39.16255057210549	41.0	41.0	41.0	37.0	41.0
42-43	39.04812816344135	41.0	41.0	41.0	37.0	41.0
44-45	39.07486406064199	41.0	41.0	41.0	37.0	41.0
46-47	39.06201851817413	41.0	41.0	41.0	37.0	41.0
48-49	39.10343473429826	41.0	41.0	41.0	37.0	41.0
50-51	39.02028380827019	41.0	41.0	41.0	37.0	41.0
52-53	39.10746942110465	41.0	41.0	41.0	37.0	41.0
54-55	38.98676399208618	41.0	41.0	41.0	37.0	41.0
56-57	38.97867161524431	41.0	41.0	41.0	37.0	41.0
58-59	38.973888579313794	41.0	41.0	41.0	37.0	41.0
60-61	38.995753598015966	41.0	41.0	41.0	37.0	41.0
62-63	39.0106961920652	41.0	41.0	41.0	37.0	41.0
64-65	39.01173132263793	41.0	41.0	41.0	37.0	41.0
66-67	38.96246724766054	41.0	41.0	41.0	37.0	41.0
68-69	38.91958091741711	41.0	41.0	41.0	37.0	41.0
70-71	38.87860338362911	41.0	41.0	41.0	34.5	41.0
72-73	38.8372086676403	41.0	41.0	41.0	32.0	41.0
74-75	38.81806570753762	41.0	41.0	41.0	32.0	41.0
76-77	37.9800277447531	41.0	39.0	41.0	32.0	41.0
78-79	38.54322526315119	41.0	39.0	41.0	32.0	41.0
80-81	38.957793746004086	41.0	41.0	41.0	34.5	41.0
82-83	38.99881436848002	41.0	41.0	41.0	37.0	41.0
84-85	38.87995397232168	41.0	41.0	41.0	32.0	41.0
86-87	38.92124310334162	41.0	41.0	41.0	32.0	41.0
88-89	38.80611518372565	41.0	41.0	41.0	32.0	41.0
90-91	38.71224406034079	41.0	41.0	41.0	32.0	41.0
92-93	38.698309644329996	41.0	41.0	41.0	32.0	41.0
94-95	38.524886266641936	41.0	41.0	41.0	32.0	41.0
96-97	38.491386049621724	41.0	37.0	41.0	32.0	41.0
98-99	38.420176879358664	41.0	37.0	41.0	32.0	41.0
100-101	37.302479450424116	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	29.0
13	125.0
14	418.0
15	1735.0
16	7159.0
17	18291.0
18	30285.0
19	40348.0
20	49105.0
21	59625.0
22	70993.0
23	85255.0
24	100530.0
25	119673.0
26	139171.0
27	162675.0
28	191427.0
29	225177.0
30	265556.0
31	314726.0
32	379051.0
33	461015.0
34	578265.0
35	746264.0
36	984688.0
37	1400148.0
38	2392869.0
39	6250783.0
40	1.8854633E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.967080222449205	21.99088665422087	10.124838382774316	30.91719474055561
2	29.57862391745687	25.30821643555917	29.071049950891396	16.04210969609256
3	21.044409107100424	27.448506287245998	29.330975344686962	22.176109260966616
4	24.946845669461627	31.666856911439332	23.00041323345586	20.385884185643178
5	27.238194403647743	33.30515843025574	21.24195994628267	18.214687219813854
6	22.563763656009197	37.0173816708109	21.470714093235213	18.94814057994469
7	21.782178684735932	21.564540049048787	36.419563998258795	20.233717267956482
8	22.847157831144827	24.7493530336281	26.681388950246475	25.722100184980594
9	23.290645548894815	23.351331398616963	29.250072622600154	24.107950429888067
10-11	25.072185089516648	30.07213896203378	23.237233821212765	21.61844212723681
12-13	25.4242508501876	24.936800722132617	26.33401244986721	23.304935977812566
14-15	24.562635602022837	26.579749519224755	26.399397548518955	22.458217330233456
16-17	25.414655718022633	26.259896279649166	25.793381196699894	22.532066805628315
18-19	24.91380651389347	26.734091733924824	25.992837996422313	22.359263755759393
20-21	25.07828978929253	26.720817644623896	25.83306324996767	22.367829316115905
22-23	25.116030307348396	26.535434390279995	25.919535902645386	22.428999399726226
24-25	24.669096083565588	26.573966993165282	26.312034936889873	22.444901986379257
26-27	24.804855357374738	26.762490886382235	26.06993920442669	22.362714551816335
28-29	25.054331775312534	26.464968406309186	25.9774487836768	22.50325103470148
30-31	24.642611378557373	26.4661441644245	26.376312502000665	22.514931955017463
32-33	24.7322648558001	26.730063855706167	26.177150856722104	22.360520431771636
34-35	25.035922128729833	26.61678316397742	25.844899874149128	22.502394833143626
36-37	24.67526086346898	26.532946017647912	26.35789438376045	22.43389873512266
38-39	24.804442006354876	26.752169646388158	26.139653681304825	22.30373466595214
40-41	25.041176362310548	26.46207864057013	26.022547251809137	22.47419774531019
42-43	24.555197139700283	26.626485300196894	26.403071915251914	22.415245644850902
44-45	24.71795213551626	26.645581756230303	26.214279668789686	22.422186439463754
46-47	25.056853429768367	26.45692237481252	26.028468066584313	22.457756128834802
48-49	24.654874167978054	26.462546032178523	26.404158113505183	22.478421686338237
50-51	24.83789103644892	26.54399114271127	26.113686243657558	22.50443157718225
52-53	25.073331489398377	26.498173572993	26.036606235353304	22.39188870225532
54-55	24.577264874917304	26.532652705382333	26.440478216745294	22.44960420295507
56-57	24.645368055248614	26.65302145631388	26.287214724211378	22.41439576422613
58-59	25.046783350551937	26.389760732715377	26.076454018252893	22.487001898479793
60-61	24.723139535982106	26.497773063258833	26.350255718295763	22.428831682463297
62-63	24.77815220694091	26.55712608028956	26.300025009160866	22.364696703608665
64-65	24.96681117814758	26.431939811275956	26.21103812547996	22.390210885096508
66-67	24.6813405383126	26.491238034565896	26.48961694665262	22.337804480468883
68-69	24.73734856022829	26.707765867057233	26.259164552140845	22.295721020573627
70-71	25.15102267154954	26.510414614210926	26.006266886383884	22.332295827855656
72-73	24.70579138154447	26.516702385278606	26.505521329650488	22.271984903526445
74-75	24.680072530683177	26.75935992590293	26.3388881816964	22.221679361717488
76-77	25.075954916279485	26.483969006412178	26.14222475268958	22.297851324618755
78-79	24.686080470923176	26.59526194333834	26.430739843781677	22.287917741956807
80-81	24.950240585683268	26.68855564637813	26.267197109555944	22.094006658382654
82-83	25.196603909179345	26.36679106042627	26.166955994578906	22.26964903581548
84-85	24.900606340088355	26.55845697861196	26.42264093745951	22.11829574384018
86-87	24.9637679995447	26.778975527937376	26.142713512092115	22.11454296042581
88-89	25.238988591869845	26.56544430472231	26.015917515715053	22.179649587692793
90-91	24.84349022277917	26.649353109818996	26.368985842108767	22.13817082529307
92-93	25.037738118152177	26.785451001903855	26.08606519324021	22.090745686703762
94-95	25.427303792443528	26.674771938819763	25.911340191447817	21.986584077288896
96-97	25.106957609973545	26.59840824434635	26.26740344916104	22.027230696519062
98-99	25.242470968771478	26.871190578806537	25.941173484916412	21.945164967505573
100-101	25.642967135085243	26.593316365830844	25.829513473907934	21.934203025175982
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68.0
1	44.0
2	22.0
3	31.0
4	47.0
5	60.0
6	65.0
7	79.0
8	97.5
9	119.0
10	152.5
11	196.0
12	228.5
13	263.5
14	332.5
15	414.5
16	492.5
17	595.5
18	756.5
19	991.0
20	1290.0
21	1659.0
22	2151.5
23	2873.0
24	3841.0
25	5145.0
26	6916.5
27	9543.5
28	13471.0
29	18981.5
30	26890.0
31	38361.5
32	56359.5
33	83680.0
34	126562.5
35	195168.5
36	294081.5
37	430961.0
38	626362.0
39	877316.5
40	1140528.5
41	1404920.5
42	1678370.0
43	1910847.5
44	2082734.0
45	2192367.0
46	2229797.5
47	2211703.5
48	2125032.5
49	1993275.5
50	1809346.0
51	1611750.5
52	1434133.0
53	1261804.5
54	1098246.5
55	927336.5
56	758926.5
57	602113.5
58	474538.5
59	379704.0
60	316876.5
61	272901.5
62	232485.5
63	193307.0
64	159805.0
65	132360.0
66	112595.0
67	95122.5
68	72911.0
69	52925.0
70	39936.0
71	30618.0
72	22162.5
73	15071.0
74	9512.5
75	5628.5
76	3340.5
77	1832.0
78	1066.5
79	743.0
80	560.0
81	426.5
82	331.0
83	285.5
84	244.5
85	203.0
86	174.0
87	145.5
88	123.5
89	107.0
90	103.0
91	86.5
92	73.0
93	68.0
94	62.0
95	71.0
96	72.5
97	67.5
98	79.0
99	154.5
100	689.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007760089966351036
2	0.006354255209818775
3	0.001582669317102357
4	1.355731631037401E-4
5	0.014818736175774026
6	0.0015974055304831983
7	0.005187147110056143
8	0.009481279689233301
9	0.0034983770566117275
10-11	0.006796341611244013
12-13	0.0047892693487734265
14-15	0.009736216180721857
16-17	9.87326296516368E-5
18-19	0.013134386986343862
20-21	0.006551720469122048
22-23	0.008840254407166704
24-25	0.011923070246438707
26-27	0.010178302582147095
28-29	0.006952545473080932
30-31	0.011155313529296874
32-33	0.005322720273159882
34-35	0.005210725051465488
36-37	0.00934865376880573
38-39	0.01072206885590014
40-41	0.009108453490698016
42-43	0.01280134856393685
44-45	0.00811817995150548
46-47	0.004559384420032303
48-49	0.010256404513065554
50-51	0.010639546060967427
52-53	0.0075891498911332764
54-55	0.010620388983572334
56-57	0.012724720254356475
58-59	0.006140580115796576
60-61	0.002323900850158675
62-63	0.013116703530286853
64-65	0.005315352166469462
66-67	0.006442672490103823
68-69	0.015775116424190626
70-71	0.006383727636580457
72-73	0.006175947027910594
74-75	0.014118766040184064
76-77	0.0063734122872138674
78-79	0.013219857023952742
80-81	2.2104320071261972E-5
82-83	0.007485996397467387
84-85	0.017161794103327792
86-87	0.0022340099485355434
88-89	0.010481868577792427
90-91	0.0038549934204280874
92-93	0.012110220156375391
94-95	0.010437659937649902
96-97	0.004442968334323656
98-99	0.009658114249803396
100-101	0.010598284663501073
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.3930019E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.663252414318027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.597676145193546	19.820460203349977
2	14.962370199433817	9.475146086822859
3	6.871995071692491	6.527691436348467
4	3.9962335465679115	5.061350059661805
5	2.487267678936095	3.9377492170064246
6	1.6969677271241248	3.2238910489729635
7	1.250991022704353	2.772731116395365
8	0.9242757999308508	2.3412462362924993
9	0.687477067440345	1.9590983923876906
>10	4.079123788048192	23.48303847883177
>50	0.2690321914830839	5.854282027007644
>100	0.15693606204495086	9.469512563102999
>500	0.013965606106484122	3.0255209359985407
>1k	0.005620043342076937	2.919081261251689
>5k	6.804995159316075E-5	0.1292009365693738
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9472426761682628E-5	5.894485352336526E-6	8.841728028504788E-6	0.0	0.0
2	4.420864014252394E-5	2.0630698733177838E-5	1.4736213380841314E-5	2.947242676168263E-6	0.0
3	9.725900831355267E-5	2.0630698733177838E-5	1.7683456057009577E-5	5.894485352336526E-6	2.947242676168263E-6
4	1.9451801662710534E-4	2.0630698733177838E-5	1.7683456057009577E-5	5.894485352336526E-6	2.947242676168263E-6
5	2.0925423000794666E-4	2.3577941409346103E-5	2.6525184085514364E-5	8.841728028504788E-6	2.947242676168263E-6
6	2.6230459817897536E-4	2.6525184085514364E-5	2.9472426761682628E-5	1.1788970704673051E-5	2.947242676168263E-6
7	2.711463262074802E-4	2.6525184085514364E-5	3.8314154790187415E-5	1.4736213380841314E-5	2.947242676168263E-6
8	3.1240772367383587E-4	2.6525184085514364E-5	3.8314154790187415E-5	2.0630698733177838E-5	8.841728028504788E-6
9	3.4188015043551845E-4	2.6525184085514364E-5	4.420864014252394E-5	2.0630698733177838E-5	8.841728028504788E-6
10-11	4.1850846001589327E-4	2.6525184085514364E-5	5.599761084719699E-5	2.0630698733177838E-5	1.1788970704673051E-5
12-13	5.069257403009412E-4	3.094604809976676E-5	7.957555225654309E-5	2.0630698733177838E-5	2.2104320071261972E-5
14-15	5.968166419240732E-4	3.6840533452103284E-5	9.431176563738441E-5	2.6525184085514364E-5	2.3577941409346103E-5
16-17	7.029173782661306E-4	4.1261397466355676E-5	1.2820505641331943E-4	2.7998805423598494E-5	2.3577941409346103E-5
18-19	8.045972505939357E-4	4.420864014252394E-5	1.4883575514649725E-4	2.9472426761682628E-5	2.3577941409346103E-5
20-21	9.43117656373844E-4	5.15767468329446E-5	1.6209834718925446E-4	2.9472426761682628E-5	2.3577941409346103E-5
22-23	0.0011376356730009494	6.483933887570177E-5	1.7241369655584336E-4	2.9472426761682628E-5	2.3577941409346103E-5
24-25	0.0014529906393509536	6.778658155187005E-5	1.8567628859860055E-4	2.9472426761682628E-5	2.6525184085514364E-5
26-27	0.0019157077395093708	6.926020288995418E-5	1.9157077395093707E-4	3.094604809976676E-5	2.9472426761682628E-5
28-29	0.0024476850425577423	7.368106690420657E-5	2.1809595803645143E-4	3.8314154790187415E-5	3.5366912114019154E-5
30-31	0.0031167091300479378	9.725900831355266E-5	2.313585500792086E-4	4.1261397466355676E-5	3.8314154790187415E-5
32-33	0.0040023555542365	1.1641608570864638E-4	2.3283217141729275E-4	4.420864014252394E-5	3.8314154790187415E-5
34-35	0.005226934886184414	1.2083694972289877E-4	2.534628701504706E-4	4.420864014252394E-5	3.9787776128271545E-5
36-37	0.006719713301663639	1.267314350752353E-4	2.637782195170595E-4	4.420864014252394E-5	4.4208640142523944E-5
38-39	0.008738574534838898	1.3852040577990834E-4	2.873561609264056E-4	4.420864014252394E-5	4.7155882818692205E-5
40-41	0.011420565370152017	1.4441489113224486E-4	3.124077236738358E-4	4.7155882818692205E-5	4.7155882818692205E-5
42-43	0.014885049135987812	1.5325661916074966E-4	3.6103722783061216E-4	5.0103125494860466E-5	5.305036817102873E-5
44-45	0.018625100092045335	1.5915110451308618E-4	3.7724706254953764E-4	5.599761084719699E-5	5.8944853523365256E-5
46-47	0.023118171551863853	1.6209834718925446E-4	4.288238093824822E-4	5.599761084719699E-5	7.073382422803831E-5
48-49	0.028557307910732382	1.7094007521775923E-4	4.5682261480608073E-4	5.599761084719699E-5	7.368106690420657E-5
50-51	0.03600056928939533	1.7978180324626402E-4	4.6124347882033306E-4	5.599761084719699E-5	7.368106690420657E-5
52-53	0.044887979579380725	1.8420266726051644E-4	4.921895269200999E-4	5.599761084719699E-5	7.368106690420657E-5
54-55	0.05513259512174161	1.8567628859860055E-4	5.025048762866888E-4	5.599761084719699E-5	7.51546882422907E-5
56-57	0.06640285111540904	1.8862353127476882E-4	5.231355750198667E-4	5.599761084719699E-5	7.662830958037483E-5
58-59	0.07980838442796037	1.930443952890212E-4	5.39345409738792E-4	5.599761084719699E-5	7.810193091845895E-5
60-61	0.09623778872626036	1.9451801662710534E-4	5.820804285432319E-4	5.8944853523365256E-5	8.104917359462723E-5
62-63	0.11684343589669077	1.9599163796518948E-4	6.248154473476716E-4	6.189209619953351E-5	8.252279493271135E-5
64-65	0.14144407051466729	2.0778060866986252E-4	6.631296021378591E-4	6.189209619953351E-5	8.841728028504789E-5
66-67	0.16985843715560547	2.1809595803645146E-4	6.646032234759432E-4	6.189209619953351E-5	9.283814429930027E-5
68-69	0.2027938740617858	2.313585500792086E-4	6.73444951504448E-4	6.189209619953351E-5	9.725900831355267E-5
70-71	0.24248144393906765	2.402002781077134E-4	6.793394368567845E-4	6.189209619953351E-5	1.031534936658892E-4
72-73	0.2912774673070475	2.4314752078388168E-4	6.984965142518782E-4	6.189209619953351E-5	1.1494246437056224E-4
74-75	0.3520098235135088	2.4756838479813404E-4	6.984965142518782E-4	6.631296021378591E-5	1.1494246437056224E-4
76-77	0.4244692583284436	2.4756838479813404E-4	7.029173782661306E-4	6.778658155187005E-5	1.1494246437056224E-4
78-79	0.5090448077851062	2.6083097684089125E-4	7.102854849565512E-4	6.778658155187005E-5	1.1788970704673051E-4
80-81	0.6093704221032119	2.740935688836484E-4	7.147063489708037E-4	6.778658155187005E-5	1.267314350752353E-4
82-83	0.732279283427457	2.7998805423598496E-4	7.235480769993085E-4	6.778658155187005E-5	1.2967867775140357E-4
84-85	0.8828760160729647	2.91777024940658E-4	7.29442562351645E-4	6.926020288995418E-5	1.2967867775140357E-4
86-87	1.065392860522713	2.976715102929945E-4	7.500732610848228E-4	7.368106690420657E-5	1.3262592042757182E-4
88-89	1.2785389244845398	3.0798685965958343E-4	7.574413677752435E-4	7.368106690420657E-5	1.355731631037401E-4
90	1.4587171318707484	3.241966943785089E-4	7.662830958037483E-4	7.368106690420657E-5	1.4146764845607662E-4
>>END_MODULE
