##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654109_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34618062
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.472800326026338	32.0	32.0	32.0	32.0	32.0
2	31.50859580181005	32.0	32.0	32.0	32.0	32.0
3	34.95397356443581	37.0	32.0	37.0	32.0	37.0
4	35.88609593454423	37.0	37.0	37.0	32.0	37.0
5	36.061844045458116	37.0	37.0	37.0	37.0	37.0
6	39.50374284383684	41.0	41.0	41.0	37.0	41.0
7	39.62168523471938	41.0	41.0	41.0	37.0	41.0
8	39.73449131843371	41.0	41.0	41.0	37.0	41.0
9	39.67293509960205	41.0	41.0	41.0	37.0	41.0
10-11	39.667608270503415	41.0	41.0	41.0	37.0	41.0
12-13	39.75050038907435	41.0	41.0	41.0	37.0	41.0
14-15	39.706867833907054	41.0	41.0	41.0	37.0	41.0
16-17	39.61383232544907	41.0	41.0	41.0	37.0	41.0
18-19	39.61099409608776	41.0	41.0	41.0	37.0	41.0
20-21	39.689830513909186	41.0	41.0	41.0	37.0	41.0
22-23	39.60530039203235	41.0	41.0	41.0	37.0	41.0
24-25	39.56303791644952	41.0	41.0	41.0	37.0	41.0
26-27	39.531031633139946	41.0	41.0	41.0	37.0	41.0
28-29	39.37011475396861	41.0	41.0	41.0	37.0	41.0
30-31	39.51424509263401	41.0	41.0	41.0	37.0	41.0
32-33	39.59645823038852	41.0	41.0	41.0	37.0	41.0
34-35	39.54118093034786	41.0	41.0	41.0	37.0	41.0
36-37	39.361260373269886	41.0	41.0	41.0	37.0	41.0
38-39	39.27414118387101	41.0	41.0	41.0	37.0	41.0
40-41	39.26571519226004	41.0	41.0	41.0	37.0	41.0
42-43	39.23824382485652	41.0	41.0	41.0	37.0	41.0
44-45	39.2385066934134	41.0	41.0	41.0	37.0	41.0
46-47	39.18534123891742	41.0	41.0	41.0	37.0	41.0
48-49	39.15636188704036	41.0	41.0	41.0	37.0	41.0
50-51	39.15964354099314	41.0	41.0	41.0	37.0	41.0
52-53	39.17414651923612	41.0	41.0	41.0	37.0	41.0
54-55	39.179742716388915	41.0	41.0	41.0	37.0	41.0
56-57	39.1420410680413	41.0	41.0	41.0	37.0	41.0
58-59	39.11380449315736	41.0	41.0	41.0	37.0	41.0
60-61	39.06991153924214	41.0	41.0	41.0	37.0	41.0
62-63	39.00927790238518	41.0	41.0	41.0	37.0	41.0
64-65	39.03228752377877	41.0	41.0	41.0	37.0	41.0
66-67	39.02814487419891	41.0	41.0	41.0	37.0	41.0
68-69	38.98043930073266	41.0	41.0	41.0	37.0	41.0
70-71	38.9080727569325	41.0	41.0	41.0	34.5	41.0
72-73	38.92339914926491	41.0	41.0	41.0	37.0	41.0
74-75	38.82574606573874	41.0	41.0	41.0	32.0	41.0
76-77	37.896769264553285	41.0	39.0	41.0	29.5	41.0
78-79	38.450020830166636	41.0	39.0	41.0	32.0	41.0
80-81	38.9266802254846	41.0	41.0	41.0	34.5	41.0
82-83	39.00636036471366	41.0	41.0	41.0	37.0	41.0
84-85	38.97898745747234	41.0	41.0	41.0	37.0	41.0
86-87	38.93257973251073	41.0	41.0	41.0	34.5	41.0
88-89	38.859942694076864	41.0	41.0	41.0	32.0	41.0
90-91	38.74596141459334	41.0	41.0	41.0	32.0	41.0
92-93	38.6671740318681	41.0	41.0	41.0	32.0	41.0
94-95	38.531724768417135	41.0	41.0	41.0	32.0	41.0
96-97	38.567353784853694	41.0	41.0	41.0	32.0	41.0
98-99	38.51588997096371	41.0	39.0	41.0	32.0	41.0
100-101	37.47563919089404	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	31.0
13	102.0
14	370.0
15	1549.0
16	6811.0
17	17914.0
18	30648.0
19	41846.0
20	51584.0
21	62595.0
22	75388.0
23	89725.0
24	105929.0
25	123829.0
26	143342.0
27	166220.0
28	194249.0
29	227117.0
30	265482.0
31	314863.0
32	375223.0
33	451361.0
34	557773.0
35	703819.0
36	932767.0
37	1331055.0
38	2229804.0
39	5781223.0
40	2.0335443E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	35.046596178338504	20.166597394099504	12.771410409306396	32.0153960182556
2	30.51250866299915	22.809036154497928	29.937239297530798	16.741215884972128
3	22.23971352117883	26.281152031205174	28.67891813144821	22.800216316167784
4	24.917517895351743	31.870857605612237	22.673006310188292	20.538618188847728
5	27.040173537656546	33.13162687046684	21.551046245876634	18.277153345999974
6	22.073991910953236	36.35607138965655	21.798314826217684	19.77162187317254
7	21.408130819108244	19.60106258981222	38.02797510732981	20.962831483749724
8	23.13991895050277	24.047583171372548	26.233573434511094	26.578924443613595
9	23.225309377515124	23.39532756050873	28.821471866333827	24.55789119564232
10-11	25.656196467612773	29.525494523639136	22.57454071230215	22.243768296445943
12-13	25.798769460413943	24.128216613699916	26.058171834136395	24.014842091749745
14-15	24.545995671277034	26.651910785762645	26.481109774429314	22.320983768531004
16-17	25.46796114620189	26.31076770011478	25.74358916288736	22.477681990795972
18-19	25.015678231785476	26.90438014698801	25.80520827537948	22.274733345847032
20-21	25.189666550065226	26.85568348109132	25.717842335757524	22.236807633085927
22-23	25.263049463356	26.647524732140187	25.84023805030215	22.24918775420166
24-25	24.82727221886752	26.659817350806758	26.146263094611665	22.366647335714056
26-27	25.00317373377628	26.776135746396534	25.989298208705513	22.231392311121674
28-29	25.21837979004371	26.49809254532421	25.884313123424228	22.39921454120785
30-31	24.854625847035848	26.648899031589075	26.121533217956944	22.374941903418136
32-33	24.935937387634873	26.868221505293626	25.955676010934287	22.24016509613721
34-35	25.217294081915963	26.67515732105396	25.824228693102462	22.28331990392761
36-37	24.900424812919912	26.581395573212617	26.158096313999323	22.360083299868144
38-39	25.05315733289402	26.768739433980333	25.890178694288213	22.287924538837434
40-41	25.234757508955873	26.57159577563874	25.86377452325321	22.329872192152177
42-43	24.822971569119176	26.71430985942671	26.174088430862913	22.288630140591202
44-45	24.994976509476572	26.704299322155205	26.005381287841555	22.295342880526665
46-47	25.260885488043783	26.560672287200827	25.888076865770245	22.290365358985145
48-49	24.851528176014735	26.57498397270676	26.17534952180704	22.39813832947146
50-51	25.101254420273694	26.64360461821983	25.91041306141616	22.344727900090312
52-53	25.295064727919957	26.626669067741627	25.90862864071079	22.16963756362762
54-55	24.80458295723251	26.65927211337455	26.239798878164567	22.29634605122838
56-57	24.88957643046092	26.740728523856873	26.14061503009711	22.229080015585097
58-59	25.268171280724488	26.45476729886224	25.994228843951085	22.282832576462187
60-61	24.964478080835377	26.579922353827897	26.171423172100162	22.28417639323657
62-63	25.046495000130935	26.70375202543341	26.042521257255945	22.20723171717971
64-65	25.179649357315974	26.5942230762979	26.084510826812185	22.141616739573937
66-67	24.876058126648235	26.648850730264805	26.34499253541624	22.130098607670718
68-69	25.048324267879636	26.814713813929842	26.075841531787614	22.061120386402912
70-71	25.393074759620227	26.6477922070269	25.854930867966992	22.104202165385882
72-73	24.917524835420142	26.6757071473546	26.324408659461145	22.082359357764116
74-75	24.895551018783426	26.904307597541134	26.154212083254833	22.04592930042061
76-77	25.27965506689252	26.69461141101085	25.98151332349653	22.0442201986001
78-79	24.92589504653834	26.762773805650752	26.267481802258004	22.043849345552903
80-81	25.15036294444064	26.786059306410976	26.14506393206428	21.9185138170841
82-83	25.37560700566098	26.57562344881306	26.06579906988149	21.98297047564446
84-85	25.081332619617285	26.736789494779945	26.25772918964944	21.92414869595334
86-87	25.150651125415397	26.94281528526929	26.003065971746192	21.903467617569117
88-89	25.4071646182851	26.779555135119924	25.92512111163242	21.88815913496255
90-91	25.065487490316475	26.803997577911787	26.218979271572163	21.91153566019958
92-93	25.28435300624281	26.958685613307875	25.935843837820848	21.821117542628468
94-95	25.595143723524195	26.85879680873376	25.850532202922892	21.69552726481915
96-97	25.318046000458942	26.83274933535507	26.151959610029525	21.697245054156458
98-99	25.523627680937995	27.06232160143227	25.835418685061533	21.5786320325682
100-101	25.846973595746604	26.87453337889167	25.730772112227466	21.54772091313426
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	44.5
2	23.0
3	20.0
4	23.5
5	31.5
6	42.0
7	54.0
8	72.0
9	95.5
10	121.0
11	164.5
12	210.0
13	247.0
14	307.5
15	393.0
16	501.5
17	645.5
18	781.5
19	1001.0
20	1387.0
21	1866.0
22	2366.0
23	3121.5
24	4264.5
25	5799.5
26	7971.5
27	10845.5
28	14956.0
29	20797.5
30	28917.0
31	40222.5
32	57263.0
33	82022.5
34	119236.0
35	179299.5
36	266797.0
37	391361.5
38	572250.5
39	812805.0
40	1085984.5
41	1375482.0
42	1684391.5
43	1951987.5
44	2148354.0
45	2274182.0
46	2322905.0
47	2315621.0
48	2229448.5
49	2074367.0
50	1872958.5
51	1670621.5
52	1485438.5
53	1302522.0
54	1129091.0
55	955823.5
56	784472.5
57	626747.0
58	496749.0
59	400348.5
60	335888.5
61	286309.5
62	238574.5
63	194370.0
64	163104.0
65	137447.0
66	113890.0
67	92062.5
68	68597.0
69	50296.5
70	39930.5
71	30454.5
72	19902.5
73	12534.0
74	6865.0
75	3264.0
76	1892.0
77	1114.0
78	737.5
79	549.5
80	439.0
81	370.5
82	310.0
83	236.5
84	210.5
85	207.5
86	187.0
87	149.5
88	126.5
89	115.5
90	96.5
91	82.0
92	70.5
93	64.5
94	55.5
95	54.0
96	60.0
97	69.0
98	85.5
99	147.0
100	693.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017320438099625565
2	0.007083007708519327
3	6.210630739525512E-4
4	0.0015974319995151662
5	2.888665460244424E-4
6	0.0011150248676543475
7	0.0
8	0.024111690596660205
9	0.0
10-11	0.0
12-13	0.003980581004216816
14-15	0.0
16-17	0.0011294681949555697
18-19	0.0
20-21	3.943028353233639E-4
22-23	1.4443327301222118E-6
24-25	1.7331992761466544E-5
26-27	0.005472576714433061
28-29	9.604812655312709E-4
30-31	1.7765292580503207E-4
32-33	4.954061264319187E-4
34-35	0.0
36-37	0.0
38-39	8.882646290251603E-4
40-41	0.0
42-43	1.0110329110855483E-4
44-45	0.002016288491250608
46-47	0.0
48-49	0.0015916546685946775
50-51	0.0012637911388569355
52-53	6.759477176971952E-4
54-55	6.918353777285395E-4
56-57	0.008501342449499339
58-59	6.196187412224289E-4
60-61	0.0
62-63	0.004926618942446865
64-65	1.4443327301222118E-6
66-67	0.002679237214376703
68-69	2.9897687513529784E-4
70-71	8.37712983470883E-5
72-73	1.8776325491588755E-4
74-75	5.892877538898624E-4
76-77	2.9897687513529784E-4
78-79	2.874222132943202E-4
80-81	0.001656649641450177
82-83	0.005280480461326806
84-85	5.38736108335585E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	4.7662980094032996E-5
96-97	0.004756187680292443
98-99	2.8886654602444237E-6
100-101	5.011834573524076E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4618062E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.471646184925994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.644351368815485	19.08577569393693
2	15.705213664524592	9.885378554171659
3	7.38786464913288	6.975247868988974
4	4.210388904743139	5.300314796440565
5	2.6649107513202397	4.193456413997794
6	1.7418106754044773	3.2890589578474017
7	1.296964521653803	2.857232597792319
8	0.9348781785836193	2.353772420991338
9	0.7178637593924706	2.033311882012261
>10	4.246647452527134	24.326550704730344
>50	0.27574627750203323	5.968336637641711
>100	0.15839534632549226	9.326595802043663
>500	0.010952022336008138	2.338360351827133
>1k	0.0039929301536345855	2.028421676924864
>5k	1.949758490451185E-5	0.0381856406531941
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.332998190366636E-5	2.8886654602444237E-6	0.0	0.0	0.0
2	4.621864736391078E-5	2.888665460244424E-5	0.0	5.777330920488847E-6	2.8886654602444237E-6
3	7.22166365061106E-5	3.177532006268866E-5	2.8886654602444237E-6	8.665996380733272E-6	5.777330920488847E-6
4	1.1554661840977695E-4	3.177532006268866E-5	1.7331992761466544E-5	1.444332730122212E-5	5.777330920488847E-6
5	1.2998994571099907E-4	3.755265098317751E-5	2.5997989142199814E-5	1.7331992761466544E-5	5.777330920488847E-6
6	1.7620859307490987E-4	3.755265098317751E-5	4.332998190366636E-5	1.7331992761466544E-5	5.777330920488847E-6
7	1.8487458945564312E-4	4.0441316443421937E-5	6.355064012537732E-5	2.0220658221710968E-5	5.777330920488847E-6
8	2.2242724043882063E-4	4.0441316443421937E-5	6.643930558562175E-5	2.5997989142199814E-5	5.777330920488847E-6
9	2.3975923320028718E-4	4.0441316443421937E-5	6.643930558562175E-5	2.5997989142199814E-5	5.777330920488847E-6
10-11	3.1197586970639775E-4	4.332998190366636E-5	8.232696561696608E-5	2.5997989142199814E-5	1.1554661840977695E-5
12-13	3.639718479907974E-4	4.332998190366636E-5	9.67702929181882E-5	2.5997989142199814E-5	1.1554661840977695E-5
14-15	4.4485448087764126E-4	5.1995978284399635E-5	1.1987961660014359E-4	2.5997989142199814E-5	1.1554661840977695E-5
16-17	5.762887593187626E-4	5.4884643744644056E-5	1.819859239953987E-4	2.888665460244424E-5	1.1554661840977695E-5
18-19	6.961683759189061E-4	5.4884643744644056E-5	1.8920758764600975E-4	3.466398552293308E-5	1.2998994571099907E-5
20-21	8.11714994328683E-4	5.4884643744644056E-5	2.1087257859784295E-4	3.899698371329972E-5	1.444332730122212E-5
22-23	0.0010095885783554261	5.777330920488848E-5	2.310932368195539E-4	4.332998190366636E-5	1.444332730122212E-5
24-25	0.0013345634426329238	6.643930558562175E-5	2.3831490047016495E-4	4.910731282415521E-5	1.7331992761466544E-5
26-27	0.0017346436088767764	6.932797104586618E-5	2.4409223139065382E-4	5.344031101452184E-5	1.7331992761466544E-5
28-29	0.002176609424294173	8.088263288684387E-5	2.686458878027314E-4	5.4884643744644056E-5	2.0220658221710968E-5
30-31	0.0028467798110708795	8.954862926757715E-5	2.7297888599309806E-4	5.6328976474766266E-5	2.4553656412077603E-5
32-33	0.0035718348415922302	1.0254762383867705E-4	2.802005496437091E-4	6.355064012537732E-5	2.888665460244424E-5
34-35	0.004447100476046291	1.0976928748928811E-4	3.235305315473755E-4	6.355064012537732E-5	3.177532006268866E-5
36-37	0.005539016020018683	1.1265795294953253E-4	3.365295261184754E-4	6.355064012537732E-5	3.177532006268866E-5
38-39	0.0069241311082058835	1.1699095113989916E-4	3.423068570389642E-4	6.355064012537732E-5	3.177532006268866E-5
40-41	0.00886098129929977	1.3143427844112128E-4	3.6830484618116405E-4	6.355064012537732E-5	3.177532006268866E-5
42-43	0.011369787251522052	1.3576727663148792E-4	4.08746162624586E-4	6.355064012537732E-5	3.899698371329972E-5
44-45	0.01436533333379552	1.42988940282099E-4	4.2318948992580807E-4	6.355064012537732E-5	4.332998190366636E-5
46-47	0.017590528320158418	1.4732193847246563E-4	4.578534754487412E-4	6.355064012537732E-5	5.344031101452184E-5
48-49	0.021623105302659637	1.689869294242988E-4	4.7951846640057437E-4	6.355064012537732E-5	5.4884643744644056E-5
50-51	0.027388881561307504	1.8487458945564312E-4	4.8674013005118547E-4	6.355064012537732E-5	5.4884643744644056E-5
52-53	0.03475064548674042	1.8776325491588755E-4	5.228484483042408E-4	6.355064012537732E-5	5.4884643744644056E-5
54-55	0.04308011234135521	1.8776325491588755E-4	5.618454320175405E-4	6.499497285549954E-5	5.6328976474766266E-5
56-57	0.05232961914505786	1.9065192037613198E-4	6.109527448416956E-4	6.643930558562175E-5	5.921764193501069E-5
58-59	0.06317078061735518	1.9787358402674303E-4	6.326177357935288E-4	6.643930558562175E-5	6.06619746651329E-5
60-61	0.07708981513754294	2.0220658221710967E-4	6.802807158875618E-4	6.643930558562175E-5	6.355064012537732E-5
62-63	0.09521907956603694	2.1087257859784295E-4	7.42387023282817E-4	6.643930558562175E-5	6.643930558562175E-5
64-65	0.11771889483588077	2.1376124405808735E-4	7.756066760756279E-4	6.643930558562175E-5	7.077230377598839E-5
66-67	0.14378043461820594	2.166499095183318E-4	7.828283397262388E-4	6.643930558562175E-5	7.36609692362328E-5
68-69	0.1741821942545484	2.166499095183318E-4	7.929386688370944E-4	6.643930558562175E-5	7.510530196635502E-5
70-71	0.211617854286586	2.166499095183318E-4	7.958273342973388E-4	6.643930558562175E-5	7.510530196635502E-5
72-73	0.25920572907865264	2.2676023862918727E-4	8.146036597889275E-4	6.643930558562175E-5	7.510530196635502E-5
74-75	0.31952250822128636	2.2964890408943168E-4	8.146036597889275E-4	7.510530196635502E-5	7.510530196635502E-5
76-77	0.3945079305710412	2.310932368195539E-4	8.24713988899783E-4	7.510530196635502E-5	7.510530196635502E-5
78-79	0.486332828221291	2.3975923320028718E-4	8.276026543600275E-4	7.510530196635502E-5	7.510530196635502E-5
80-81	0.5974294574895613	2.4409223139065382E-4	8.348243180106385E-4	7.799396742659944E-5	7.510530196635502E-5
82-83	0.7332617290939047	2.556468932316315E-4	8.521563107721051E-4	7.943830015672166E-5	7.799396742659944E-5
84-85	0.9021894408762685	2.5997989142199815E-4	8.564893089624717E-4	8.088263288684387E-5	8.232696561696608E-5
86-87	1.1137090805372063	2.686458878027314E-4	8.709326362636938E-4	8.232696561696608E-5	8.665996380733272E-5
88-89	1.3688981202933892	2.744232187232203E-4	8.752656344540604E-4	8.37712983470883E-5	8.665996380733272E-5
90	1.5885580192212956	2.85977880564198E-4	8.781542999143049E-4	8.37712983470883E-5	8.665996380733272E-5
>>END_MODULE
