##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654125_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39688479
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3981228910284	32.0	32.0	32.0	32.0	32.0
2	31.308246985227125	32.0	32.0	32.0	32.0	32.0
3	35.013591803303925	37.0	32.0	37.0	32.0	37.0
4	35.895669068094044	37.0	37.0	37.0	32.0	37.0
5	36.09341819322429	37.0	37.0	37.0	37.0	37.0
6	39.13705705376112	41.0	41.0	41.0	37.0	41.0
7	39.261853899717345	41.0	41.0	41.0	37.0	41.0
8	39.46193846330065	41.0	41.0	41.0	37.0	41.0
9	39.67377633695663	41.0	41.0	41.0	37.0	41.0
10-11	39.69032581218343	41.0	41.0	41.0	37.0	41.0
12-13	39.60355855410836	41.0	41.0	41.0	37.0	41.0
14-15	39.494502774974066	41.0	41.0	41.0	37.0	41.0
16-17	39.57080115113507	41.0	41.0	41.0	37.0	41.0
18-19	39.562526848156615	41.0	41.0	41.0	37.0	41.0
20-21	39.4988828496048	41.0	41.0	41.0	37.0	41.0
22-23	39.38434836467278	41.0	41.0	41.0	37.0	41.0
24-25	39.42887006327453	41.0	41.0	41.0	37.0	41.0
26-27	39.301998774001895	41.0	41.0	41.0	37.0	41.0
28-29	39.418352792002935	41.0	41.0	41.0	37.0	41.0
30-31	39.387157945760535	41.0	41.0	41.0	37.0	41.0
32-33	39.39569061086972	41.0	41.0	41.0	37.0	41.0
34-35	39.252042563787846	41.0	41.0	41.0	37.0	41.0
36-37	39.182169742508904	41.0	41.0	41.0	37.0	41.0
38-39	39.142317963356575	41.0	41.0	41.0	37.0	41.0
40-41	39.083675151673106	41.0	41.0	41.0	37.0	41.0
42-43	39.061642876261395	41.0	41.0	41.0	37.0	41.0
44-45	39.06521668416671	41.0	41.0	41.0	37.0	41.0
46-47	39.01694213325736	41.0	41.0	41.0	37.0	41.0
48-49	38.967667972360445	41.0	41.0	41.0	37.0	41.0
50-51	39.01983527763812	41.0	41.0	41.0	37.0	41.0
52-53	39.0155547281114	41.0	41.0	41.0	37.0	41.0
54-55	39.00288842008786	41.0	41.0	41.0	37.0	41.0
56-57	38.965232202524064	41.0	41.0	41.0	37.0	41.0
58-59	38.86968730900471	41.0	41.0	41.0	32.0	41.0
60-61	38.907911059025466	41.0	41.0	41.0	34.5	41.0
62-63	38.90306985561226	41.0	41.0	41.0	34.5	41.0
64-65	38.87741516876976	41.0	41.0	41.0	32.0	41.0
66-67	38.836103331145544	41.0	41.0	41.0	32.0	41.0
68-69	38.81524555274592	41.0	41.0	41.0	32.0	41.0
70-71	38.7870474955717	41.0	41.0	41.0	32.0	41.0
72-73	38.757344543236336	41.0	41.0	41.0	32.0	41.0
74-75	38.73321852419691	41.0	41.0	41.0	32.0	41.0
76-77	37.85100028650632	41.0	39.0	41.0	29.5	41.0
78-79	38.31395343973751	41.0	39.0	41.0	32.0	41.0
80-81	38.658239523363946	41.0	41.0	41.0	32.0	41.0
82-83	38.82425887371497	41.0	41.0	41.0	32.0	41.0
84-85	38.77688111958133	41.0	41.0	41.0	32.0	41.0
86-87	38.71365157379803	41.0	41.0	41.0	32.0	41.0
88-89	38.65344702426112	41.0	41.0	41.0	32.0	41.0
90-91	38.595997178425506	41.0	41.0	41.0	32.0	41.0
92-93	38.52730241942504	41.0	41.0	41.0	32.0	41.0
94-95	38.51080705309972	41.0	41.0	41.0	32.0	41.0
96-97	38.40743311176021	41.0	37.0	41.0	32.0	41.0
98-99	38.20031630589824	41.0	37.0	41.0	32.0	41.0
100-101	37.21290180961583	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	34.0
13	146.0
14	532.0
15	2096.0
16	8711.0
17	24115.0
18	42563.0
19	58738.0
20	72565.0
21	86954.0
22	102426.0
23	120468.0
24	140441.0
25	163031.0
26	188131.0
27	217190.0
28	250603.0
29	292013.0
30	339867.0
31	398609.0
32	471450.0
33	565004.0
34	688400.0
35	862175.0
36	1123503.0
37	1579135.0
38	2627564.0
39	6684631.0
40	2.2577384E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.391442265764017	20.226597978522154	12.47432519161218	38.90763456410165
2	27.112895911178658	24.668370385269743	32.252964393016924	15.965769310534677
3	20.531555186995323	27.61151553407129	28.987308927662742	22.869620351270644
4	24.289731536449153	30.696862935966884	23.726565585947498	21.28683994163646
5	27.681129423271766	32.27766015177886	22.398331121924237	17.642879303025136
6	22.132327116894555	37.21453019149462	22.23064028228444	18.422502409326395
7	21.62872757104146	21.748812797789505	36.47363508185839	20.148824549310646
8	22.271941940632193	24.212570605187462	28.594723924794398	24.920763529385944
9	23.316842149581998	22.626238712750872	30.6266435657562	23.430275571910933
10-11	24.570361717405188	30.0817813553819	24.03546468228766	21.312392244925256
12-13	25.21653581176002	24.998577351386196	26.627886384591992	23.15700045226179
14-15	24.458133051659654	26.70048151757088	26.54870447416239	22.29268095660708
16-17	25.43572395595659	26.243574605329982	25.95940327497745	22.36129816373597
18-19	24.96007939837939	26.884283198891467	25.95032675423912	22.205310648490016
20-21	25.277516006078528	26.81323126996091	25.843686176219343	22.065566547741224
22-23	24.98669035625147	26.59305812269363	26.188760410727813	22.231491110327088
24-25	24.505573172736405	26.714179469345183	26.615671806887793	22.164575551030623
26-27	24.814403948309533	26.790552996500573	26.36620440909313	22.02883864609677
28-29	24.91782507030976	26.49556507473583	26.298610565705538	22.287999289248877
30-31	24.617006612926637	26.53230928804301	26.487511652940892	22.363172446089454
32-33	24.858982842597026	26.763262984306923	26.25551819209175	22.122235981004305
34-35	24.982999474482252	26.65580734399018	26.05814271693304	22.303050464594524
36-37	24.674213390742437	26.673124712085844	26.464071601232185	22.188590295939534
38-39	24.980271202726367	26.826017841089104	26.249785401810016	21.94392555437451
40-41	25.074892640758545	26.483199318371458	26.185226196247026	22.256681844622968
42-43	24.45932350927869	26.696197748306467	26.653644720325996	22.190834022088847
44-45	24.76753569964748	26.679613356504994	26.459991054757747	22.09285988908978
46-47	24.98326500290883	26.52221576375624	26.31837723781374	22.176141995521192
48-49	24.662425938894934	26.57599558535701	26.54578488182569	22.215793593922363
50-51	24.964723389878458	26.594716567495567	26.2781410696036	22.162418973022373
52-53	25.143263160861355	26.488665744967115	26.184934250134578	22.183136844036948
54-55	24.57191368037519	26.59899413898859	26.61755503100392	22.2115371496323
56-57	24.755129451157572	26.746867722129906	26.432572837536195	22.06542998917633
58-59	25.04122166032112	26.451098062992035	26.292893461601285	22.214786815085557
60-61	24.76081653645126	26.578013204692436	26.46417786809453	22.19699239076178
62-63	24.926264554016647	26.599134571352735	26.46171353078127	22.012887343849346
64-65	25.013528485180803	26.474869445110254	26.379242197716874	22.132359871992072
66-67	24.696054249813102	26.62537911801471	26.636411556179766	22.042155075992422
68-69	24.90281173631865	26.82686843357422	26.337661354677895	21.932658475429243
70-71	25.183595452911028	26.551887453338217	26.208129091490427	22.05638800226032
72-73	24.752816806106377	26.630463717191077	26.610529720728277	22.00618975597427
74-75	24.800100180455843	26.832235278940587	26.45046106694201	21.917203473661562
76-77	25.128054979136145	26.521247293355476	26.296236896388507	22.054460831119872
78-79	24.678146522067525	26.772431868704267	26.546930659650624	22.002490949577584
80-81	25.072468257975117	26.765043647886618	26.36766021679443	21.79482787734383
82-83	25.196625082947055	26.43075226224734	26.3452162630246	22.027406391781003
84-85	24.892469871803407	26.634210577671126	26.600316919499228	21.87300263102624
86-87	25.035557548073335	26.855265730893844	26.27802768657373	21.831149034459095
88-89	25.142116697257933	26.590436035432862	26.294027067906967	21.973420199402234
90-91	24.778439085241004	26.747856420597984	26.583447187784248	21.890257306376768
92-93	25.10114154123922	26.872427304897144	26.287362176221308	21.739068977642326
94-95	25.401668831904594	26.61506378211168	26.24453685917266	21.738730526811068
96-97	25.031504251174695	26.70279113819739	26.51222988315641	21.753474727471502
98-99	25.24252005683263	26.981370156118388	26.17417224700272	21.601937540046265
100-101	25.529900140573652	26.60855998339653	26.18897064109231	21.6725692349375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	16.5
2	16.0
3	26.0
4	37.0
5	58.5
6	86.0
7	105.0
8	120.0
9	145.5
10	179.0
11	221.5
12	285.0
13	360.5
14	450.0
15	606.5
16	797.0
17	938.5
18	1161.0
19	1501.5
20	1964.0
21	2582.5
22	3387.0
23	4539.0
24	6050.5
25	8045.5
26	10940.0
27	14857.0
28	20515.0
29	28570.0
30	39794.0
31	56203.0
32	81189.0
33	118284.5
34	176147.0
35	265704.0
36	390782.0
37	561739.0
38	799959.0
39	1099222.0
40	1409971.5
41	1715071.0
42	2028603.0
43	2290784.5
44	2481957.0
45	2601910.0
46	2616116.0
47	2563794.5
48	2452613.5
49	2278969.5
50	2049040.5
51	1821758.0
52	1609694.5
53	1401896.0
54	1212274.0
55	1027265.5
56	846687.0
57	673540.5
58	532811.5
59	420080.0
60	345289.5
61	298234.5
62	256956.0
63	213276.0
64	173384.0
65	145454.0
66	125702.0
67	105945.0
68	79773.5
69	57456.5
70	44462.5
71	35459.5
72	26075.0
73	18055.0
74	11646.5
75	6774.5
76	3843.0
77	2080.5
78	1250.5
79	801.5
80	541.5
81	405.0
82	326.5
83	274.0
84	228.0
85	182.0
86	145.5
87	122.5
88	101.0
89	89.0
90	76.0
91	65.0
92	62.5
93	57.5
94	52.0
95	43.5
96	44.0
97	49.0
98	61.5
99	127.0
100	582.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04235486071411303
2	0.0
3	2.6959964880488366E-4
4	0.0
5	0.008503727240340956
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.3432492840050635E-4
12-13	4.598311766999184E-4
14-15	0.0
16-17	0.0011338302987121275
18-19	2.8975663189309922E-5
20-21	0.0014815382569838467
22-23	3.8172286723308296E-4
24-25	0.0030512633149786367
26-27	0.0
28-29	0.0022374251227919315
30-31	0.0
32-33	0.004806180655096407
34-35	0.0
36-37	0.0
38-39	0.002357107209878212
40-41	0.0
42-43	8.856474444384729E-4
44-45	8.617110270212169E-4
46-47	0.0017435790371306493
48-49	0.0014072093818460517
50-51	0.0
52-53	0.0032440144657596985
54-55	4.8502740556018793E-4
56-57	0.0034947169429193794
58-59	0.0
60-61	3.0235474632323398E-5
62-63	0.0033385003239857084
64-65	0.0
66-67	0.002441514576560115
68-69	4.371545707256758E-4
70-71	0.002906384999032087
72-73	0.0
74-75	2.4944266571666804E-4
76-77	0.0035879429897023767
78-79	0.0
80-81	0.004083048886806672
82-83	0.0012875272947597716
84-85	0.002805600083591009
86-87	0.0
88-89	0.001029265948942009
90-91	3.653453184739078E-5
92-93	4.094387189793794E-4
94-95	0.0
96-97	0.002464191182534357
98-99	2.267660597424255E-5
100-101	9.776136797784566E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.9688479E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.291315797346982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.012503252311824	18.16427816147473
2	14.725000544043718	8.626292821033815
3	6.7393115188604495	5.922099058669188
4	4.0530773498890476	4.748798744266972
5	2.526984036103132	3.7009343708175657
6	1.7807622281396756	3.12965212706559
7	1.304155938659	2.674031041377238
8	0.9951020105924502	2.3318277794270705
9	0.7693417537176329	2.028152903780514
>10	4.548215761596762	24.566572904926616
>50	0.3332548598266704	6.731403525942506
>100	0.18893000850279246	10.53903458095301
>500	0.01614312988498337	3.241808016249629
>1k	0.007117950116612612	3.3968641533898376
>5k	9.965775549261257E-5	0.19824981062596184
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.51177373161617E-5	0.0	5.0392457720539E-6	2.51962288602695E-6	2.51962288602695E-6
2	2.267660597424255E-5	7.55886865808085E-6	1.00784915441078E-5	2.51962288602695E-6	2.51962288602695E-6
3	5.0392457720539E-5	7.55886865808085E-6	1.259811443013475E-5	2.51962288602695E-6	1.00784915441078E-5
4	1.209418985292936E-4	1.51177373161617E-5	2.267660597424255E-5	2.51962288602695E-6	1.00784915441078E-5
5	1.410988816175092E-4	1.763736020218865E-5	2.51962288602695E-5	5.0392457720539E-6	1.00784915441078E-5
6	1.662951104777787E-4	1.763736020218865E-5	3.02354746323234E-5	5.0392457720539E-6	1.00784915441078E-5
7	1.864520935659943E-4	2.01569830882156E-5	3.275509751835035E-5	7.55886865808085E-6	1.259811443013475E-5
8	2.2928568262845246E-4	2.01569830882156E-5	4.53532119484851E-5	1.51177373161617E-5	1.763736020218865E-5
9	2.51962288602695E-4	2.01569830882156E-5	5.795132637861985E-5	1.51177373161617E-5	1.763736020218865E-5
10-11	3.099136149813149E-4	2.01569830882156E-5	7.306906369478155E-5	1.51177373161617E-5	2.267660597424255E-5
12-13	3.716443756889751E-4	2.267660597424255E-5	8.692698956792977E-5	1.51177373161617E-5	3.149528607533688E-5
14-15	4.484928737127971E-4	2.51962288602695E-5	8.818680101094326E-5	1.51177373161617E-5	3.275509751835035E-5
16-17	5.417189204957942E-4	3.02354746323234E-5	1.1842227564326665E-4	1.51177373161617E-5	3.52747204043773E-5
18-19	6.878570478853574E-4	3.149528607533688E-5	1.423586930605227E-4	2.3936417417256023E-5	3.779434329040425E-5
20-21	8.226568722877992E-4	3.779434329040425E-5	1.763736020218865E-4	2.771585174629645E-5	4.03139661764312E-5
22-23	9.763538683354432E-4	4.157377761944467E-5	2.2298662541338508E-4	2.771585174629645E-5	4.03139661764312E-5
24-25	0.0012686301231145693	4.53532119484851E-5	2.2550624829941203E-4	2.8975663189309926E-5	4.283358906245815E-5
26-27	0.0016730295963218948	5.417189204957942E-5	2.3054549407146595E-4	3.02354746323234E-5	4.661302339149857E-5
28-29	0.002090027183959355	6.04709492646468E-5	2.4440341994461416E-4	3.02354746323234E-5	5.417189204957943E-5
30-31	0.0027174132825800654	7.55886865808085E-5	2.4692304283064113E-4	3.02354746323234E-5	6.173076070766027E-5
32-33	0.0034216478792245984	8.944661245395673E-5	2.4944266571666804E-4	3.02354746323234E-5	6.55101950367007E-5
34-35	0.004193912293791858	9.07064238969702E-5	2.922762547791262E-4	3.02354746323234E-5	6.55101950367007E-5
36-37	0.005059402755142116	9.57456696690241E-5	3.124332378673418E-4	3.02354746323234E-5	7.810830946683545E-5
38-39	0.006297797403624362	1.00784915441078E-4	3.1999210652542266E-4	3.149528607533688E-5	8.06279323528624E-5
40-41	0.007912875673567635	1.0834378409915885E-4	3.439285239426787E-4	3.275509751835035E-5	8.06279323528624E-5
42-43	0.009973927194337682	1.0960359554217233E-4	3.779434329040425E-4	3.275509751835035E-5	9.07064238969702E-5
44-45	0.012242847603204949	1.2850076718737445E-4	3.8676211300513685E-4	3.275509751835035E-5	9.196623533998367E-5
46-47	0.014846877855913803	1.373194472884688E-4	4.2581626773855456E-4	3.275509751835035E-5	9.448585822601063E-5
48-49	0.0179787691032453	1.4361850450353615E-4	4.384143821686893E-4	3.275509751835035E-5	9.57456696690241E-5
50-51	0.02207441610448211	1.5747643037668439E-4	4.434536279407432E-4	3.52747204043773E-5	9.57456696690241E-5
52-53	0.027242162643723384	1.662951104777787E-4	4.5101249659882406E-4	3.9054154733417725E-5	9.700548111203758E-5
54-55	0.03282942639348815	1.6881473336380565E-4	4.661302339149857E-4	4.03139661764312E-5	1.00784915441078E-4
56-57	0.039069272470733885	1.700745448068191E-4	4.925862742182687E-4	4.03139661764312E-5	1.0330453832710495E-4
58-59	0.04637995827454108	1.713343562498326E-4	5.051843886484035E-4	4.283358906245815E-5	1.0330453832710495E-4
60-61	0.05595956448721555	1.7511379057887303E-4	5.354198632807269E-4	5.417189204957942E-5	1.0708397265614538E-4
62-63	0.0675208540997502	1.8015303635092694E-4	6.034496812034546E-4	5.795132637861985E-5	1.1338302987121276E-4
64-65	0.08180711586352302	1.9401096222407515E-4	6.5258232748098E-4	5.795132637861985E-5	1.209418985292936E-4
66-67	0.09876291807504137	2.1038851098325033E-4	6.601411961390609E-4	5.795132637861985E-5	1.2346152141532056E-4
68-69	0.11837314299698912	2.2424643685639854E-4	6.727393105691957E-4	5.795132637861985E-5	1.2346152141532056E-4
70-71	0.14213948586943834	2.3810436272954678E-4	6.777785563412495E-4	5.795132637861985E-5	1.2850076718737445E-4
72-73	0.17234850446145844	2.431436085016007E-4	7.004551623154921E-4	6.04709492646468E-5	1.423586930605227E-4
74-75	0.20937814220595352	2.456632313876276E-4	7.02974785201519E-4	6.425038359368723E-5	1.4865775027559007E-4
76-77	0.25490898756790353	2.4944266571666804E-4	7.092738424165864E-4	6.55101950367007E-5	1.4865775027559007E-4
78-79	0.31037470596945776	2.570015343747489E-4	7.143130881886404E-4	6.55101950367007E-5	1.51177373161617E-4
80-81	0.3769494416755049	2.6456040303282977E-4	7.155728996316538E-4	6.55101950367007E-5	1.562166189336709E-4
82-83	0.45920505041274073	2.6959964880488366E-4	7.193523339606943E-4	6.55101950367007E-5	1.562166189336709E-4
84-85	0.5622966806059764	2.7463889457693755E-4	7.256513911757616E-4	6.677000647971418E-5	1.562166189336709E-4
86-87	0.6899080209145833	2.7463889457693755E-4	7.29430825504802E-4	6.802981792272766E-5	1.5747643037668436E-4
88-89	0.8440295734185228	2.771585174629645E-4	7.31950448390829E-4	6.802981792272766E-5	1.5999605326271133E-4
90	0.9774297473077767	3.02354746323234E-4	7.332102598338425E-4	6.802981792272766E-5	1.612558647057248E-4
>>END_MODULE
