##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654129_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27646524
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.61361345100744	32.0	32.0	32.0	32.0	32.0
2	31.67810817736074	32.0	32.0	32.0	32.0	32.0
3	35.69221732902118	37.0	37.0	37.0	32.0	37.0
4	36.339801813783176	37.0	37.0	37.0	37.0	37.0
5	36.422568095721545	37.0	37.0	37.0	37.0	37.0
6	40.066015966419506	41.0	41.0	41.0	37.0	41.0
7	40.083142459428174	41.0	41.0	41.0	37.0	41.0
8	40.2208730833576	41.0	41.0	41.0	37.0	41.0
9	40.199435126093974	41.0	41.0	41.0	37.0	41.0
10-11	40.26929130403518	41.0	41.0	41.0	41.0	41.0
12-13	40.22991664340877	41.0	41.0	41.0	41.0	41.0
14-15	40.260929023120596	41.0	41.0	41.0	41.0	41.0
16-17	40.26227213952828	41.0	41.0	41.0	41.0	41.0
18-19	40.25597668263829	41.0	41.0	41.0	41.0	41.0
20-21	40.1877032353145	41.0	41.0	41.0	41.0	41.0
22-23	40.14503110770815	41.0	41.0	41.0	39.0	41.0
24-25	40.18168477527229	41.0	41.0	41.0	39.0	41.0
26-27	40.15472341116012	41.0	41.0	41.0	41.0	41.0
28-29	40.071504685362974	41.0	41.0	41.0	39.0	41.0
30-31	40.085041378077044	41.0	41.0	41.0	37.0	41.0
32-33	40.097828790339065	41.0	41.0	41.0	37.0	41.0
34-35	40.089905262592865	41.0	41.0	41.0	37.0	41.0
36-37	40.07447771734341	41.0	41.0	41.0	37.0	41.0
38-39	40.04848667412945	41.0	41.0	41.0	37.0	41.0
40-41	40.03858617090525	41.0	41.0	41.0	37.0	41.0
42-43	39.98325246964139	41.0	41.0	41.0	37.0	41.0
44-45	40.001337437574435	41.0	41.0	41.0	37.0	41.0
46-47	39.98479116217287	41.0	41.0	41.0	37.0	41.0
48-49	39.96496371478744	41.0	41.0	41.0	37.0	41.0
50-51	39.89363836480854	41.0	41.0	41.0	37.0	41.0
52-53	39.91520575968248	41.0	41.0	41.0	37.0	41.0
54-55	39.863927342185946	41.0	41.0	41.0	37.0	41.0
56-57	39.862510762655006	41.0	41.0	41.0	37.0	41.0
58-59	39.85627558459067	41.0	41.0	41.0	37.0	41.0
60-61	39.77964309364896	41.0	41.0	41.0	37.0	41.0
62-63	39.74346105861265	41.0	41.0	41.0	37.0	41.0
64-65	39.733138205728864	41.0	41.0	41.0	37.0	41.0
66-67	39.69943534311945	41.0	41.0	41.0	37.0	41.0
68-69	39.61703091498953	41.0	41.0	41.0	37.0	41.0
70-71	39.63657805588869	41.0	41.0	41.0	37.0	41.0
72-73	39.54602036046195	41.0	41.0	41.0	37.0	41.0
74-75	39.58842142325017	41.0	41.0	41.0	37.0	41.0
76-77	39.04648503008913	41.0	39.0	41.0	34.5	41.0
78-79	39.365658319288166	41.0	41.0	41.0	37.0	41.0
80-81	39.578366180862375	41.0	41.0	41.0	37.0	41.0
82-83	39.718965194322436	41.0	41.0	41.0	37.0	41.0
84-85	39.67933695751408	41.0	41.0	41.0	37.0	41.0
86-87	39.69586764325237	41.0	41.0	41.0	37.0	41.0
88-89	39.634521757599614	41.0	41.0	41.0	37.0	41.0
90-91	39.651826193412234	41.0	41.0	41.0	37.0	41.0
92-93	39.60421934055797	41.0	41.0	41.0	37.0	41.0
94-95	39.4636535139101	41.0	41.0	41.0	37.0	41.0
96-97	39.425951034567674	41.0	41.0	41.0	37.0	41.0
98-99	39.47564111495536	41.0	41.0	41.0	37.0	41.0
100-101	38.93960474018361	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	3.0
16	10.0
17	17.0
18	47.0
19	149.0
20	538.0
21	1519.0
22	3693.0
23	7860.0
24	14747.0
25	25397.0
26	40404.0
27	62635.0
28	91095.0
29	125064.0
30	164015.0
31	207107.0
32	254908.0
33	309005.0
34	372844.0
35	455264.0
36	578248.0
37	784734.0
38	1234728.0
39	2783330.0
40	2.0129161E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.89891476268871	11.049307091052448	6.803588253085581	51.248189893173254
2	21.69410158036504	13.112563445589037	35.256370746644315	29.936964227401607
3	20.25885785858649	14.798909258899961	20.95241340285672	43.98981947965683
4	23.48417333019696	22.13831905021386	20.25654480075325	34.120962818835935
5	24.659613628100228	27.447161169339047	23.954845100960974	23.93838010159975
6	20.265043084620693	31.540771635522784	25.293791002442116	22.900394277414403
7	15.597508750105437	25.948694309635457	39.57249019804443	18.881306742214683
8	17.910692859615914	24.56957699275323	32.89214947962355	24.627580668007308
9	18.26778855450716	22.450958341493106	35.15354711253963	24.127705991460108
10-11	20.597157778129453	31.567732688264417	25.48363077340447	22.35147876020166
12-13	21.198633787583603	25.50761560405681	27.76716485567368	25.526585752685904
14-15	20.818998800717225	26.25839870502346	27.54632553445055	25.376276959808763
16-17	21.44118768259445	26.532507467814238	26.838738219991683	25.187566629599633
18-19	21.169106185393055	26.7276261502132	26.70282373847978	25.40044392591396
20-21	21.45789787172875	26.702367238821335	26.92326556894563	24.916469320504287
22-23	21.301941972885995	26.971164620912198	26.947427459596728	24.77946594660508
24-25	21.22379471647141	26.644731178501864	26.83591977060118	25.295554334425553
26-27	21.121266456499196	26.74051718038767	27.093981145694844	25.044235217418294
28-29	21.22623928977577	26.918625806974344	26.8647274445065	24.99040745874339
30-31	21.245122895015665	26.53339710988622	26.93656533457877	25.284914660519348
32-33	21.368565784866085	26.559774297672522	26.958463495458346	25.11319642200305
34-35	21.45193256280595	26.68660625169055	26.689036952659134	25.17242423284437
36-37	21.301345590012595	26.486168327927416	26.644830809483576	25.567655272576406
38-39	21.405235664629053	26.65072654483526	26.933443326576494	25.01059446395919
40-41	21.528947970333974	26.81944461952182	26.747762868339258	24.903844541804947
42-43	21.522861246498838	26.448616108122668	26.68679614117131	25.341726504207184
44-45	21.532709915951383	26.47042487705521	26.904280315980216	25.092584891013185
46-47	21.515242408455983	26.64013223389209	26.70653854782713	25.1380868098248
48-49	21.464822748509683	26.39654392396546	26.722696902569442	25.415936424955415
50-51	21.517426863830057	26.421571733895398	26.89214465824839	25.16885674402615
52-53	21.86435444832052	26.60634298908608	26.53635227343589	24.99295028915751
54-55	21.716731241060167	26.363094403465553	26.532451736677686	25.387722618796595
56-57	21.62691911243976	26.32643574819889	26.910011810200945	25.1366333291604
58-59	21.656022421648828	26.50027355362151	26.74125502397399	25.102449000755673
60-61	21.519484371685554	26.2706692728793	26.75230341514977	25.45754294028538
62-63	21.712531047401555	26.23754468749607	26.94699960602517	25.102924659077214
64-65	21.82638152178771	26.407151266682423	26.680575178970035	25.085892032559837
66-67	21.684852203732323	26.21567301582777	26.67531518707868	25.424159593361228
68-69	21.713877817208754	26.25229530279711	26.87549410177969	25.158332778214447
70-71	21.852478760268617	26.46794902449998	26.66780301185978	25.01176920337162
72-73	21.846149839504115	26.311426853040892	26.535736773184958	25.306686534270035
74-75	21.789396165112777	26.289605479907813	26.836817129584205	25.084181225395202
76-77	21.8013284418685	26.411557199740553	26.681506145221007	25.105608213169944
78-79	21.705166413047728	26.16056866828887	26.72972237764379	25.404542541019616
80-81	21.790564374447975	26.22137073422265	26.85480661545259	25.133258275876795
82-83	21.867456022671455	26.4624941169253	26.55755476500022	25.112495095403027
84-85	21.937409216525104	26.162601336354452	26.546755089006354	25.35323435811409
86-87	21.934199045187878	26.17206456299021	26.779944901133756	25.113791490688154
88-89	22.05839040857125	26.345175536028094	26.687208284470117	24.909225770930547
90-91	21.98733885483534	26.112821098245618	26.62235741516935	25.277482631749688
92-93	21.991909926773157	26.118752997796218	26.756077624061085	25.13325945136954
94-95	22.167533056119577	26.287477903740765	26.486534160498433	25.05845487964123
96-97	22.097279795091158	26.21625154186855	26.339365912791518	25.347102750248773
98-99	22.102291896899136	26.32341490115041	26.6317133799138	24.94257982203665
100-101	22.121130713369134	26.462308523335054	26.505772648672938	24.91078811462287
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	25.0
2	19.5
3	21.5
4	34.5
5	53.0
6	67.5
7	86.0
8	103.0
9	120.5
10	148.5
11	186.5
12	216.5
13	246.5
14	299.5
15	355.0
16	429.0
17	541.0
18	722.0
19	934.5
20	1267.5
21	1778.5
22	2519.0
23	3686.5
24	5670.0
25	8432.5
26	12074.0
27	17703.5
28	25693.5
29	36950.5
30	52387.5
31	72354.5
32	99547.0
33	135077.0
34	180871.0
35	242901.5
36	317251.0
37	404640.5
38	522947.5
39	680841.5
40	863937.0
41	1063159.5
42	1269066.5
43	1460798.5
44	1622191.5
45	1733334.5
46	1788584.0
47	1783336.5
48	1710695.5
49	1589807.5
50	1447008.0
51	1291230.0
52	1144375.0
53	1009204.0
54	863916.5
55	722234.0
56	599493.0
57	496977.0
58	409318.5
59	334138.0
60	274839.0
61	233430.0
62	199822.5
63	166231.5
64	145224.5
65	128310.5
66	109258.5
67	92080.0
68	66864.0
69	46797.5
70	38731.5
71	29961.5
72	22204.5
73	20401.0
74	17692.5
75	12310.5
76	7358.0
77	2446.5
78	338.5
79	112.0
80	44.0
81	16.0
82	8.0
83	2.5
84	1.5
85	2.0
86	1.5
87	1.0
88	0.5
89	1.0
90	1.0
91	0.0
92	0.0
93	0.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.5
99	0.5
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03379448353073247
2	0.0
3	0.0
4	0.019062070877337058
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0026838817060690886
10-11	0.0032517650320163212
12-13	0.0016530107003686974
14-15	0.0
16-17	7.776746183353828E-4
18-19	6.872473371335941E-5
20-21	3.43623668566797E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.591520149151481E-4
30-31	0.0
32-33	0.002000251460183566
34-35	6.438422421567356E-4
36-37	3.671347616792621E-4
38-39	0.0043025300395811055
40-41	6.329909684125209E-5
42-43	0.0
44-45	0.0024578135030646165
46-47	2.0436565551604243E-4
48-49	5.118184116021241E-4
50-51	4.3405094976858574E-5
52-53	0.0
54-55	8.87995901401565E-4
56-57	3.0745275608608154E-4
58-59	7.505464339748462E-4
60-61	7.415037058546673E-5
62-63	3.183040298302962E-4
64-65	0.0022588734844206817
66-67	0.0020328052814162097
68-69	0.008303032959948237
70-71	0.008471227702983565
72-73	6.384166052846281E-4
74-75	0.0013220468511701508
76-77	0.0
78-79	0.0014088570411238679
80-81	0.008567080621057462
82-83	0.005573937613278255
84-85	1.5010928679496922E-4
86-87	0.009986788935925544
88-89	1.7542892553147006E-4
90-91	0.0021865316594592505
92-93	6.275653315404136E-4
94-95	0.013875162027602458
96-97	0.0010109770038359976
98-99	3.671347616792621E-4
100-101	0.015144761055675571
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.7646524E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.84131040522458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.96075665990287	22.924373332006144
2	15.875417966265504	11.37991566283202
3	6.579556588045992	7.074597900026902
4	3.5453211215347973	5.082758192125104
5	2.1422848319022223	3.8391147818305953
6	1.5074546294985438	3.241748957859008
7	1.0699696464271662	2.6844379955235125
8	0.7849622732956063	2.2507261194862846
9	0.6244818471143373	2.014402295236767
>10	3.549099490736237	23.120591039262443
>50	0.23189622885523503	5.692852275454128
>100	0.11976444118090221	7.714710602521967
>500	0.006609146493353374	1.5930777264295612
>1k	0.0024251287470246027	1.3866931194055208
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.978800372878703E-5	0.0	0.0	0.0	0.0
2	5.063927747300167E-5	0.0	3.6170912480715477E-6	0.0	0.0
3	6.872473371335941E-5	0.0	3.6170912480715477E-6	0.0	3.255382123264393E-5
4	1.0851273744214643E-4	0.0	3.6170912480715477E-6	0.0	3.255382123264393E-5
5	1.1936401118636107E-4	0.0	3.6170912480715477E-6	0.0	3.255382123264393E-5
6	1.7000328865936275E-4	0.0	3.255382123264393E-5	0.0	3.255382123264393E-5
7	1.9894001864393513E-4	3.6170912480715477E-6	3.617091248071548E-5	0.0	3.255382123264393E-5
8	2.4596220486886527E-4	7.234182496143095E-6	3.617091248071548E-5	0.0	3.255382123264393E-5
9	2.7128184360536607E-4	1.0851273744214643E-5	3.617091248071548E-5	0.0	3.978800372878703E-5
10-11	2.8575020859765226E-4	3.255382123264393E-5	3.617091248071548E-5	0.0	4.15965493528228E-5
12-13	3.327723948225824E-4	5.4256368721073216E-5	3.617091248071548E-5	0.0	5.063927747300167E-5
14-15	4.2500822164840687E-4	5.4256368721073216E-5	3.617091248071548E-5	0.0	5.4256368721073216E-5
16-17	4.8107313599351587E-4	5.606491434510899E-5	3.617091248071548E-5	0.0	5.606491434510899E-5
18-19	5.317124134665175E-4	5.787345996914476E-5	3.978800372878703E-5	3.6170912480715477E-6	5.787345996914476E-5
20-21	6.0767132967602E-4	5.787345996914476E-5	4.3405094976858574E-5	3.6170912480715477E-6	6.872473371335941E-5
22-23	6.908644283816657E-4	5.787345996914476E-5	4.3405094976858574E-5	3.6170912480715477E-6	6.872473371335941E-5
24-25	7.75866072711347E-4	6.510764246528786E-5	5.063927747300167E-5	3.6170912480715477E-6	6.872473371335941E-5
26-27	9.133155401380658E-4	9.223582682582447E-5	5.063927747300167E-5	3.6170912480715477E-6	6.872473371335941E-5
28-29	0.001135766651894466	9.947000932196756E-5	5.787345996914477E-5	3.6170912480715477E-6	7.053327933739519E-5
30-31	0.0014341766798603686	1.0489564619407488E-4	6.691618808932364E-5	3.6170912480715477E-6	7.415037058546673E-5
32-33	0.0017741832571790941	1.1212982869021798E-4	6.872473371335941E-5	3.6170912480715477E-6	7.59589162095025E-5
34-35	0.0021377009276102848	1.2478964805846842E-4	6.872473371335941E-5	3.6170912480715477E-6	7.776746183353828E-5
36-37	0.0026603706129566234	1.3383237617864728E-4	7.053327933739519E-5	3.6170912480715477E-6	9.04272812017887E-5
38-39	0.0033711290432026824	1.392580130507546E-4	7.234182496143096E-5	3.6170912480715477E-6	9.223582682582447E-5
40-41	0.004367637682046394	1.4287510429882613E-4	7.234182496143096E-5	3.6170912480715477E-6	9.404437244986024E-5
42-43	0.005575746158902291	1.609605605391839E-4	7.234182496143096E-5	3.6170912480715477E-6	9.404437244986024E-5
44-45	0.00689417591882437	2.0436565551604246E-4	7.234182496143096E-5	7.234182496143095E-6	1.0308710057003911E-4
46-47	0.00853271825420078	2.495792961169368E-4	7.234182496143096E-5	7.234182496143095E-6	1.0489564619407488E-4
48-49	0.010598077356849634	2.495792961169368E-4	7.234182496143096E-5	7.234182496143095E-6	1.175554655623253E-4
50-51	0.013294618882286974	2.550049329890441E-4	7.234182496143096E-5	7.234182496143095E-6	1.3744946742671882E-4
52-53	0.01664585392362526	2.6766475235729455E-4	7.234182496143096E-5	7.234182496143095E-6	1.4468364992286192E-4
54-55	0.02069157048459329	2.803245717255449E-4	7.415037058546673E-5	7.234182496143095E-6	1.51917832419005E-4
56-57	0.025236445637795188	3.2915530357451083E-4	8.138455308160983E-5	7.234182496143095E-6	1.5734346929111232E-4
58-59	0.030575272319948794	3.3458094044661816E-4	9.404437244986024E-5	7.234182496143095E-6	1.663861974112912E-4
60-61	0.037588812249959525	3.43623668566797E-4	9.404437244986024E-5	9.04272812017887E-6	1.7000328865936275E-4
62-63	0.04663154037013839	3.544749423110117E-4	9.404437244986024E-5	1.0851273744214643E-5	1.7000328865936275E-4
64-65	0.0579259077922418	3.5628348793504745E-4	9.404437244986024E-5	1.0851273744214643E-5	1.7000328865936275E-4
66-67	0.07077924154226405	3.617091248071548E-4	9.404437244986024E-5	1.0851273744214643E-5	1.7000328865936275E-4
68-69	0.0864394381007898	3.6532621605522635E-4	9.947000932196756E-5	1.0851273744214643E-5	1.736203799074343E-4
70-71	0.1055937448049527	3.6532621605522635E-4	1.0489564619407488E-4	1.0851273744214643E-5	1.8447165365164894E-4
72-73	0.12938877958039136	3.6532621605522635E-4	1.1212982869021798E-4	1.0851273744214643E-5	1.935143817718278E-4
74-75	0.160056287727166	3.76177489799441E-4	1.1212982869021798E-4	1.0851273744214643E-5	1.9713147301989937E-4
76-77	0.19818585511871223	4.55753497257015E-4	1.1212982869021798E-4	1.0851273744214643E-5	2.0255710989200667E-4
78-79	0.24349354009205643	4.593705885050866E-4	1.1212982869021798E-4	1.0851273744214643E-5	2.0255710989200667E-4
80-81	0.2983449203234374	4.593705885050866E-4	1.1212982869021798E-4	1.0851273744214643E-5	2.0255710989200667E-4
82-83	0.3647945036417598	4.629876797531581E-4	1.1212982869021798E-4	1.0851273744214643E-5	2.0798274676411398E-4
84-85	0.4486079334964497	4.7564749912140853E-4	1.1212982869021798E-4	1.0851273744214643E-5	2.1340838363622132E-4
86-87	0.5537929469903703	5.009671378579093E-4	1.1212982869021798E-4	1.0851273744214643E-5	2.1340838363622132E-4
88-89	0.680821574531395	5.244782309703744E-4	1.1212982869021798E-4	1.0851273744214643E-5	2.1340838363622132E-4
90	0.7899437918488414	5.461807784588038E-4	1.1212982869021798E-4	1.0851273744214643E-5	2.1340838363622132E-4
>>END_MODULE
