##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654122_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	33557947
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.638154860903736	32.0	32.0	32.0	32.0	32.0
2	31.61192739234018	32.0	32.0	32.0	32.0	32.0
3	35.71275081279555	37.0	37.0	37.0	32.0	37.0
4	36.333700449553724	37.0	37.0	37.0	37.0	37.0
5	36.512842189064784	37.0	37.0	37.0	37.0	37.0
6	40.10074388638852	41.0	41.0	41.0	37.0	41.0
7	40.147836993723125	41.0	41.0	41.0	37.0	41.0
8	40.23336454402291	41.0	41.0	41.0	37.0	41.0
9	40.24552285632968	41.0	41.0	41.0	41.0	41.0
10-11	40.29723825179175	41.0	41.0	41.0	41.0	41.0
12-13	40.2605987636848	41.0	41.0	41.0	41.0	41.0
14-15	40.210407522843994	41.0	41.0	41.0	41.0	41.0
16-17	40.235773228320554	41.0	41.0	41.0	41.0	41.0
18-19	40.231542382494375	41.0	41.0	41.0	41.0	41.0
20-21	40.20964500599516	41.0	41.0	41.0	41.0	41.0
22-23	40.20117307831734	41.0	41.0	41.0	41.0	41.0
24-25	40.184992529489364	41.0	41.0	41.0	41.0	41.0
26-27	40.13311998794205	41.0	41.0	41.0	41.0	41.0
28-29	40.114855491606804	41.0	41.0	41.0	39.0	41.0
30-31	40.09286846421207	41.0	41.0	41.0	37.0	41.0
32-33	40.077596910204306	41.0	41.0	41.0	37.0	41.0
34-35	40.069611156487014	41.0	41.0	41.0	37.0	41.0
36-37	39.97053075088294	41.0	41.0	41.0	37.0	41.0
38-39	40.009965746712695	41.0	41.0	41.0	37.0	41.0
40-41	40.01328011811927	41.0	41.0	41.0	37.0	41.0
42-43	40.00292532794094	41.0	41.0	41.0	37.0	41.0
44-45	39.985562004135716	41.0	41.0	41.0	37.0	41.0
46-47	39.966254267580794	41.0	41.0	41.0	37.0	41.0
48-49	39.94381362185237	41.0	41.0	41.0	37.0	41.0
50-51	39.88416782468844	41.0	41.0	41.0	37.0	41.0
52-53	39.8802837819608	41.0	41.0	41.0	37.0	41.0
54-55	39.84041634012951	41.0	41.0	41.0	37.0	41.0
56-57	39.82900455740037	41.0	41.0	41.0	37.0	41.0
58-59	39.795259063374765	41.0	41.0	41.0	37.0	41.0
60-61	39.797444909249066	41.0	41.0	41.0	37.0	41.0
62-63	39.744986426017064	41.0	41.0	41.0	37.0	41.0
64-65	39.75226806931902	41.0	41.0	41.0	37.0	41.0
66-67	39.69496010885291	41.0	41.0	41.0	37.0	41.0
68-69	39.661638359462216	41.0	41.0	41.0	37.0	41.0
70-71	39.66387453618661	41.0	41.0	41.0	37.0	41.0
72-73	39.64175683929652	41.0	41.0	41.0	37.0	41.0
74-75	39.59879488456192	41.0	41.0	41.0	37.0	41.0
76-77	39.04676612070458	41.0	39.0	41.0	34.5	41.0
78-79	39.40375618031699	41.0	41.0	41.0	37.0	41.0
80-81	39.68350216716178	41.0	41.0	41.0	37.0	41.0
82-83	39.707797500246365	41.0	41.0	41.0	37.0	41.0
84-85	39.72090873139528	41.0	41.0	41.0	37.0	41.0
86-87	39.70010620733146	41.0	41.0	41.0	37.0	41.0
88-89	39.65692506457561	41.0	41.0	41.0	37.0	41.0
90-91	39.62540682241378	41.0	41.0	41.0	37.0	41.0
92-93	39.57985638990371	41.0	41.0	41.0	37.0	41.0
94-95	39.55805580120858	41.0	41.0	41.0	37.0	41.0
96-97	39.53809584954646	41.0	41.0	41.0	37.0	41.0
98-99	39.491988142182834	41.0	41.0	41.0	37.0	41.0
100-101	38.96794504443314	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	12.0
17	28.0
18	78.0
19	242.0
20	613.0
21	1787.0
22	4291.0
23	9099.0
24	17330.0
25	29993.0
26	50105.0
27	78034.0
28	113899.0
29	156577.0
30	205572.0
31	260562.0
32	321970.0
33	391125.0
34	468817.0
35	568219.0
36	707804.0
37	930745.0
38	1393303.0
39	3085919.0
40	2.4761819E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	30.24523388168385	13.202188943028162	7.389398075838756	49.16317909944923
2	22.667310369135514	16.14704558654914	35.4150657666871	25.770578277628246
3	19.594258721906957	19.41457148521672	22.67942700935361	38.31174278352271
4	22.526395908545897	27.07080978463909	21.32310120163191	29.079693105183104
5	23.096126828020797	30.8512168518533	24.29636115701595	21.756295163109947
6	18.699144497725083	32.12339539126157	25.689911841150476	23.487548269862874
7	15.499285459864396	22.91838055528248	40.996831540379986	20.58550244447314
8	18.329945036099357	23.531776439014138	31.168449697121332	26.969828827765173
9	17.98806106940928	22.056703885967757	33.962479885911975	25.992755158710988
10-11	21.7265659904642	30.206259340000745	23.516155502599727	24.55101916693533
12-13	21.932582764970693	23.90047877481897	26.533662801243473	27.63327565896686
14-15	20.607695555538637	26.170036467788677	27.452316158507927	25.769951818164767
16-17	21.565023033143238	26.53960923175664	26.418226657310118	25.477141077790012
18-19	21.306446428322925	26.61717357143451	26.482859633814904	25.593520366427658
20-21	21.607099504627026	26.52709207747423	26.669116558292437	25.19669185960631
22-23	21.450175423425037	26.881124462113252	26.535964789502763	25.132735324958944
24-25	21.24770779552307	26.572395577881373	26.50267698133973	25.677219645255832
26-27	21.16501346163995	26.524758800054126	26.957420845798463	25.352806892507456
28-29	21.333902899116158	26.822330266553358	26.698081686252443	25.14568514807804
30-31	21.3943689701876	26.418181958508963	26.68477454833575	25.502674522967688
32-33	21.536698479999206	26.48207609149345	26.586191531465442	25.395033897041902
34-35	21.56766855874347	26.642006816721146	26.35891867263237	25.431405951903013
36-37	21.38687149127448	26.530282081916397	26.371134980337146	25.71171144647198
38-39	21.729805163587628	26.628024950394014	26.535276427965037	25.10689345805332
40-41	21.919696100598767	26.68105262815988	26.333358533524116	25.06589273771724
42-43	21.776957928922172	26.210459179758523	26.3004900150775	25.712092876241805
44-45	21.633647612590842	26.343932779916486	26.563658676736097	25.45876093075658
46-47	21.577864998714016	26.528473270429803	26.540209685652105	25.35345204520408
48-49	21.581768694014567	26.242466501302957	26.384049953949805	25.79171485073267
50-51	21.792544400883642	26.35807399064073	26.392261123721305	25.457120484754327
52-53	22.272958175897948	26.412596098325086	26.07440496881409	25.240040756962873
54-55	21.88586357800732	26.25430423380787	26.12854564672863	25.73128654145619
56-57	21.722113989869523	26.185710943521066	26.621256061939665	25.470919004669746
58-59	21.702769838691264	26.255795683806284	26.586435695842773	25.454998781659675
60-61	21.7626409029134	26.012252775773202	26.406645197931805	25.818461123381592
62-63	22.01220324950153	26.071289462373848	26.49010083960142	25.42640644852321
64-65	21.955689065245856	26.43403960319742	26.16041589194953	25.449855439607195
66-67	21.929430009529487	26.118935404481093	26.26400834353782	25.6876262424516
68-69	21.90892815940141	26.126291337190562	26.522978297808265	25.441802205599767
70-71	22.10360633801585	26.413129504018823	26.297666540804777	25.18559761716055
72-73	22.047598517382	26.17300718375528	26.19342560281419	25.58596869604853
74-75	22.003893019139134	25.980293767824396	26.477947671089304	25.537865541947163
76-77	21.895382932692513	26.21911435762146	26.29573555259504	25.589767157090986
78-79	21.895042884703294	26.102148198520624	26.372972058042603	25.62983685873348
80-81	22.003796894965	26.157789092401867	26.566359974285675	25.27205403834746
82-83	22.130762349675322	26.34290023760989	25.993059110558818	25.53327830215597
84-85	22.237956034676376	26.110785323071163	26.03079979833093	25.62045884392153
86-87	22.26691550821877	25.963075854779955	26.39310813577151	25.376900501229766
88-89	22.385720752144127	26.21486039216364	26.27839679026686	25.121022065425368
90-91	22.29716853656155	26.0034396621462	26.248013622525836	25.451378178766422
92-93	22.18427942075621	25.972475210101535	26.279026690111174	25.564218679031075
94-95	22.48177165790708	26.09775216225237	25.980488998463574	25.439987181376978
96-97	22.386302415937422	26.130877434188687	25.902940367597576	25.57987978227631
98-99	22.40319707281259	26.17614093019457	26.230914841125415	25.18974715586743
100-101	22.455473810719113	26.448149524760854	25.88614702800502	25.210229636515013
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	34.0
1	33.5
2	28.0
3	32.5
4	47.0
5	59.0
6	66.0
7	80.5
8	115.0
9	147.5
10	181.5
11	242.5
12	294.5
13	344.0
14	397.0
15	464.0
16	560.0
17	708.5
18	909.0
19	1141.0
20	1532.5
21	2094.5
22	2875.0
23	4355.0
24	6905.5
25	9897.0
26	13698.0
27	20204.0
28	30087.5
29	43444.5
30	62623.5
31	86679.5
32	121600.0
33	167164.5
34	220445.5
35	293353.5
36	385000.5
37	490503.5
38	628326.0
39	795798.5
40	988408.0
41	1217386.0
42	1453775.5
43	1658723.5
44	1829180.0
45	1961135.0
46	2042311.0
47	2043098.5
48	1982040.5
49	1886301.5
50	1752589.5
51	1605627.5
52	1461982.0
53	1331009.0
54	1182554.0
55	1009370.0
56	866631.5
57	742165.5
58	593390.5
59	459214.5
60	372285.0
61	315012.5
62	261268.0
63	211710.5
64	182408.0
65	164974.0
66	140173.5
67	114537.0
68	84347.5
69	56714.5
70	47175.5
71	39496.0
72	28701.5
73	23166.0
74	20817.0
75	18031.0
76	12532.0
77	4350.5
78	625.5
79	165.0
80	48.5
81	27.5
82	12.5
83	4.0
84	2.0
85	1.5
86	1.5
87	1.5
88	0.5
89	1.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.5
95	1.5
96	1.0
97	0.0
98	0.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024006236138343028
2	0.0
3	0.0022349400575666922
4	0.0
5	0.0
6	0.0
7	0.0
8	8.492772218753429E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.002379466181289338
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.542651342765396E-4
26-27	0.0
28-29	6.540924568478519E-4
30-31	0.0
32-33	1.1919680307022358E-5
34-35	7.300804188051195E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	3.2779120844311485E-5
74-75	5.587350143916731E-4
76-77	0.0
78-79	0.0015704178804501958
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0017358034447101309
88-89	5.065864130484502E-5
90-91	0.0
92-93	3.7249000959444867E-4
94-95	0.0015510483999512843
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.3557947E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.649082465078457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.440809896579765	17.315737469832438
2	16.582902444823272	9.50169879304186
3	7.131267299194631	6.12912794805433
4	3.986899568305128	4.568840580494373
5	2.4535441206645032	3.5145893922312874
6	1.6318651452679882	2.80508634712019
7	1.178154120781073	2.3627124193980276
8	0.90899371560042	2.0833468734779608
9	0.6853562732174413	1.7671345550428783
>10	4.409945549351379	23.532209452253994
>50	0.3537954785371426	6.973311713580291
>100	0.21314721416250967	11.365799719405894
>500	0.015583066146039945	3.0776418242533166
>1k	0.007220068046134447	3.6760356950947157
>5k	3.623254817394797E-4	0.6600136048364519
>10k+	1.5371384073796109E-4	0.6667136118818546
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4899600383777947E-5	0.0	0.0	0.0	2.9799200767555894E-6
2	2.0859440537289124E-5	0.0	0.0	0.0	2.9799200767555894E-6
3	5.065864130484502E-5	0.0	5.959840153511179E-6	0.0	2.9799200767555894E-6
4	9.535744245617886E-5	0.0	2.0859440537289124E-5	0.0	2.9799200767555894E-6
5	1.132369629167124E-4	0.0	2.3839360614044715E-5	0.0	2.9799200767555894E-6
6	1.5495584399129064E-4	0.0	3.575904092106707E-5	0.0	2.9799200767555894E-6
7	1.6091568414480182E-4	0.0	3.575904092106707E-5	0.0	2.9799200767555894E-6
8	2.0561448529613567E-4	0.0	3.873896099782266E-5	0.0	2.9799200767555894E-6
9	2.3243376598693598E-4	2.9799200767555894E-6	3.873896099782266E-5	0.0	2.9799200767555894E-6
10-11	2.6819280690800303E-4	2.9799200767555894E-6	4.0228921036200455E-5	0.0	8.939760230266768E-6
12-13	3.158715281360925E-4	8.939760230266768E-6	4.171888107457825E-5	0.0	8.939760230266768E-6
14-15	4.171888107457825E-4	8.939760230266768E-6	4.171888107457825E-5	0.0	8.939760230266768E-6
16-17	4.946667327414278E-4	8.939760230266768E-6	4.171888107457825E-5	0.0	8.939760230266768E-6
18-19	5.66184814583562E-4	8.939760230266768E-6	4.171888107457825E-5	0.0	1.1919680307022358E-5
20-21	6.74951897385141E-4	1.1919680307022358E-5	4.320884111295604E-5	0.0	1.1919680307022358E-5
22-23	7.822290201483421E-4	1.1919680307022358E-5	4.469880115133384E-5	0.0	1.1919680307022358E-5
24-25	9.50594504485033E-4	1.638956042215574E-5	5.065864130484502E-5	0.0	1.1919680307022358E-5
26-27	0.0011055503484763236	3.128916080593369E-5	5.3638561381600606E-5	0.0	1.1919680307022358E-5
28-29	0.0013350041943865042	3.2779120844311485E-5	5.9598401535111795E-5	0.0	1.3409640345400152E-5
30-31	0.0016180966016782852	3.2779120844311485E-5	7.449800191888973E-5	0.0	1.4899600383777947E-5
32-33	0.002030815532308934	4.171888107457825E-5	8.34377621491565E-5	0.0	1.4899600383777947E-5
34-35	0.002510582664666584	4.469880115133384E-5	8.34377621491565E-5	0.0	1.7879520460533537E-5
36-37	0.003163185161476058	5.065864130484502E-5	8.34377621491565E-5	0.0	1.7879520460533537E-5
38-39	0.004072060784886513	5.065864130484502E-5	8.34377621491565E-5	0.0	1.7879520460533537E-5
40-41	0.005329587057277371	5.214860134322281E-5	8.34377621491565E-5	0.0	1.7879520460533537E-5
42-43	0.007178627464904215	5.3638561381600606E-5	8.34377621491565E-5	0.0	1.7879520460533537E-5
44-45	0.009426977162816307	6.108836157348958E-5	8.64176822259121E-5	0.0	1.7879520460533537E-5
46-47	0.011810913224220778	6.555824168862297E-5	8.64176822259121E-5	0.0	1.7879520460533537E-5
48-49	0.014764014020285567	6.555824168862297E-5	9.237752237942327E-5	0.0	1.7879520460533537E-5
50-51	0.01897613104877959	7.151808184213415E-5	9.237752237942327E-5	0.0	2.3839360614044715E-5
52-53	0.0245515615123893	7.151808184213415E-5	9.237752237942327E-5	0.0	2.3839360614044715E-5
54-55	0.030860052314880884	7.151808184213415E-5	9.684740249455665E-5	0.0	2.9799200767555894E-5
56-57	0.03746504516500965	7.747792199564532E-5	1.2664660326211255E-4	0.0	3.575904092106707E-5
58-59	0.04483885739494135	8.045784207240091E-5	1.400562436075127E-4	0.0	3.575904092106707E-5
60-61	0.054189846595800395	8.045784207240091E-5	1.430361636842683E-4	0.0	3.575904092106707E-5
62-63	0.06602757910071197	8.19478021107787E-5	1.430361636842683E-4	0.0	3.724900095944487E-5
64-65	0.08046678183263117	8.49277221875343E-5	1.5197592391453507E-4	0.0	4.0228921036200455E-5
66-67	0.09716774390280788	9.237752237942327E-5	1.5197592391453507E-4	0.0	4.171888107457825E-5
68-69	0.11720621645895085	9.237752237942327E-5	1.5346588395291286E-4	0.0	4.171888107457825E-5
70-71	0.14032145649434394	9.237752237942327E-5	1.5495584399129064E-4	0.0	4.469880115133384E-5
72-73	0.16770543203968943	9.237752237942327E-5	1.5793576406804624E-4	0.0	4.767872122808943E-5
74-75	0.20268671382072329	9.982732257131225E-5	1.5793576406804624E-4	0.0	4.767872122808943E-5
76-77	0.24608626981859172	1.132369629167124E-4	1.6091568414480182E-4	2.9799200767555894E-6	4.767872122808943E-5
78-79	0.29966821271873395	1.147269229550902E-4	1.6240564418317963E-4	2.9799200767555894E-6	4.767872122808943E-5
80-81	0.3635889883251797	1.1621688299346799E-4	1.66875524298313E-4	2.9799200767555894E-6	4.9168681266467225E-5
82-83	0.43811231956472185	1.2068676310860138E-4	1.698554443750686E-4	2.9799200767555894E-6	5.66184814583562E-5
84-85	0.5310739062791893	1.3707632353075712E-4	1.713454044134464E-4	2.9799200767555894E-6	5.66184814583562E-5
86-87	0.6499354683407779	1.4899600383777947E-4	1.8177512468209095E-4	2.9799200767555894E-6	5.959840153511179E-5
88-89	0.797368504098299	1.5495584399129064E-4	1.8475504475884655E-4	2.9799200767555894E-6	6.853816176537856E-5
90	0.9253843806356807	1.5793576406804624E-4	1.996546451426245E-4	2.9799200767555894E-6	6.853816176537856E-5
>>END_MODULE
