##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654125_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39688479
Sequences flagged as poor quality	0
Sequence length	101
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.632445728141914	32.0	32.0	32.0	32.0	32.0
2	31.615243632793284	32.0	32.0	32.0	32.0	32.0
3	35.727938024533515	37.0	37.0	37.0	32.0	37.0
4	36.34886960520709	37.0	37.0	37.0	37.0	37.0
5	36.52780095200927	37.0	37.0	37.0	37.0	37.0
6	40.10854318705436	41.0	41.0	41.0	37.0	41.0
7	40.14715504718637	41.0	41.0	41.0	37.0	41.0
8	40.234475526260404	41.0	41.0	41.0	37.0	41.0
9	40.25195659425497	41.0	41.0	41.0	41.0	41.0
10-11	40.31073939618598	41.0	41.0	41.0	41.0	41.0
12-13	40.283872128231465	41.0	41.0	41.0	41.0	41.0
14-15	40.234999658213155	41.0	41.0	41.0	41.0	41.0
16-17	40.25758211847826	41.0	41.0	41.0	41.0	41.0
18-19	40.25401059083166	41.0	41.0	41.0	41.0	41.0
20-21	40.234255109650334	41.0	41.0	41.0	41.0	41.0
22-23	40.22585506489175	41.0	41.0	41.0	41.0	41.0
24-25	40.20924639112525	41.0	41.0	41.0	41.0	41.0
26-27	40.16106208050956	41.0	41.0	41.0	41.0	41.0
28-29	40.13981080000572	41.0	41.0	41.0	41.0	41.0
30-31	40.115872656646786	41.0	41.0	41.0	39.0	41.0
32-33	40.11146516851906	41.0	41.0	41.0	39.0	41.0
34-35	40.10325085776152	41.0	41.0	41.0	37.0	41.0
36-37	40.000953311917044	41.0	41.0	41.0	37.0	41.0
38-39	40.04014081769171	41.0	41.0	41.0	37.0	41.0
40-41	40.04423050578481	41.0	41.0	41.0	37.0	41.0
42-43	40.03387378488351	41.0	41.0	41.0	37.0	41.0
44-45	40.014600307560286	41.0	41.0	41.0	37.0	41.0
46-47	39.996268161347274	41.0	41.0	41.0	37.0	41.0
48-49	39.97261614636328	41.0	41.0	41.0	37.0	41.0
50-51	39.91329448528376	41.0	41.0	41.0	37.0	41.0
52-53	39.90888131540642	41.0	41.0	41.0	37.0	41.0
54-55	39.86825161528614	41.0	41.0	41.0	37.0	41.0
56-57	39.8572044547235	41.0	41.0	41.0	37.0	41.0
58-59	39.8215356275054	41.0	41.0	41.0	37.0	41.0
60-61	39.824349416363376	41.0	41.0	41.0	37.0	41.0
62-63	39.77217593800962	41.0	41.0	41.0	37.0	41.0
64-65	39.78057442060201	41.0	41.0	41.0	37.0	41.0
66-67	39.72358210048816	41.0	41.0	41.0	37.0	41.0
68-69	39.691925671931145	41.0	41.0	41.0	37.0	41.0
70-71	39.69467814576618	41.0	41.0	41.0	37.0	41.0
72-73	39.673395571546095	41.0	41.0	41.0	37.0	41.0
74-75	39.6296121728424	41.0	41.0	41.0	37.0	41.0
76-77	39.07750286928355	41.0	39.0	41.0	34.5	41.0
78-79	39.435211664322026	41.0	41.0	41.0	37.0	41.0
80-81	39.71385051062299	41.0	41.0	41.0	37.0	41.0
82-83	39.73804808695239	41.0	41.0	41.0	37.0	41.0
84-85	39.75238556005132	41.0	41.0	41.0	37.0	41.0
86-87	39.73468785488101	41.0	41.0	41.0	37.0	41.0
88-89	39.693576314678126	41.0	41.0	41.0	37.0	41.0
90-91	39.66063802797784	41.0	41.0	41.0	37.0	41.0
92-93	39.61303672030364	41.0	41.0	41.0	37.0	41.0
94-95	39.59198115150747	41.0	41.0	41.0	37.0	41.0
96-97	39.57263417678465	41.0	41.0	41.0	37.0	41.0
98-99	39.531414708535436	41.0	41.0	41.0	37.0	41.0
100-101	39.018050540561156	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	0.0
15	3.0
16	6.0
17	26.0
18	61.0
19	214.0
20	634.0
21	1960.0
22	4786.0
23	10329.0
24	19421.0
25	33614.0
26	55943.0
27	87447.0
28	128156.0
29	176734.0
30	233668.0
31	296999.0
32	368347.0
33	447209.0
34	542135.0
35	658642.0
36	823121.0
37	1088645.0
38	1629086.0
39	3616324.0
40	2.9464966E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.7069922699029	10.931829269144032	6.245815933509889	51.115362527443175
2	21.723692157615815	13.577199569678648	34.99060520812601	29.70850306457952
3	20.37819412155775	14.82582134374365	20.61806276551932	44.17792176917928
4	23.62392874768519	22.703701998758884	20.157645749034625	33.5147235045213
5	24.761709815082607	27.83406993248595	23.78915805768218	23.61506219474926
6	20.111690347216378	32.023693828125786	25.212843253579965	22.65177257107787
7	15.625106217852288	25.806098540586554	39.98244931482509	18.58634592673607
8	18.050090617817514	24.642306609150534	32.67621631072338	24.631386462308573
9	18.203557259022197	22.44266402852072	35.28571099940615	24.068067713050933
10-11	20.662597072566072	31.837221577576706	25.457746818667452	22.04243453118977
12-13	21.297320313030895	25.676838107099044	27.745482511436126	25.28035906843394
14-15	20.754020359925466	26.653339220154145	27.460768053737795	25.131872366182595
16-17	21.482045205108516	26.838551812479384	26.803067963375465	24.87633501903663
18-19	21.21875847144457	27.0304727475195	26.574981621240763	25.175787159795167
20-21	21.470485175307424	27.0016936149153	26.813041890569806	24.71477931920747
22-23	21.28820935667502	27.195709616385148	26.849228714458924	24.66685231248091
24-25	21.277793135671843	26.892026792974782	26.728729048311656	25.101451023041726
26-27	21.151134312806494	27.109120256283948	26.89266071395681	24.847084716952747
28-29	21.1221709321447	27.27949903504076	26.701994962987115	24.896335069827423
30-31	21.27286989254489	26.925769818490654	26.64636253760191	25.154997751362558
32-33	21.426175986857647	26.972249500642185	26.716357834358718	24.88521667814145
34-35	21.57795586936872	27.092606709026267	26.461605623118185	24.867831798486826
36-37	21.39207199147138	26.897313197615862	26.417653848614354	25.292960962298405
38-39	21.414654111587396	26.994296002121924	26.697796859385818	24.89325302690486
40-41	21.518276122398138	27.352893770507052	26.446327157057343	24.682502950037467
42-43	21.505730920048613	26.903306120650278	26.547361515164138	25.043601444136975
44-45	21.52872247888361	26.99705246955924	26.672304574836442	24.801920476720714
46-47	21.501451340576693	26.98617903699459	26.60340422720659	24.908965395222125
48-49	21.4149501672765	26.807368959641913	26.53963383177269	25.238047041308892
50-51	21.455615620845535	26.800785185040727	26.777406864092722	24.966192330021013
52-53	21.802725168681825	27.041176105539343	26.29559071790078	24.860508007878053
54-55	21.60910348819364	26.89803884900704	26.379765019465722	25.113092643333594
56-57	21.533822195604927	26.855806190002895	26.754242711090036	24.856128903302142
58-59	21.57674271165695	26.891736012357644	26.64279853102962	24.888722744955786
60-61	21.57209148780935	26.6497363126463	26.6589417548604	25.11923044468396
62-63	21.586984978688655	26.643485128266065	26.72368724435119	25.045842648694094
64-65	21.676502140583416	26.9068008375932	26.53747955420514	24.879217467618247
66-67	21.58063426920442	26.685905499175217	26.507273055236002	25.226187176384364
68-69	21.645920217804264	26.720361342141636	26.802393964253458	24.83132447580065
70-71	21.869463679875462	26.950327322949313	26.562229809814582	24.617979187360646
72-73	21.882960908592437	26.64614701913649	26.409216723065082	25.061675349205988
74-75	21.862114290348263	26.694052539437735	26.624633964162342	24.81919920605166
76-77	21.71344737096123	26.90515174441429	26.574341637027715	24.807059247596765
78-79	21.626164201313998	26.672758962312653	26.567682673311122	25.133394163062224
80-81	21.763818411887236	26.704876999695554	26.679354479671545	24.851950108745662
82-83	21.91937111019044	27.008093457045806	26.35615615302365	24.716379279740096
84-85	21.968308485694298	26.74527789286155	26.290877763292464	24.995535858151683
86-87	21.904376359777448	26.71115188435597	26.566724595061547	24.817747160805034
88-89	22.049709308463502	26.700127608875345	26.513510400075468	24.736652682585685
90-91	21.972979866524994	26.52811159631489	26.486430734722788	25.012477802437328
92-93	21.971753782236576	26.538409377216688	26.552586091286674	24.937250749260063
94-95	22.14637181245404	26.681657740527804	26.348627518512313	24.823342928505845
96-97	22.102333526059287	26.613002730590914	26.23663658161352	25.04802716173628
98-99	22.092171886959942	26.76278927191944	26.46505677378062	24.679982067339996
100-101	22.212005151419383	26.879976932348555	26.283729593164807	24.624288323067255
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45.0
1	35.0
2	25.5
3	37.0
4	48.0
5	67.0
6	97.5
7	131.0
8	169.5
9	193.5
10	223.0
11	267.0
12	323.0
13	403.0
14	469.0
15	518.5
16	632.5
17	783.5
18	973.0
19	1273.0
20	1702.5
21	2277.0
22	3193.5
23	4721.0
24	7206.5
25	10622.5
26	15565.0
27	23119.5
28	32955.5
29	46917.0
30	67593.0
31	94217.0
32	133821.0
33	185111.5
34	245882.5
35	334465.0
36	457215.0
37	610545.0
38	807762.5
39	1064617.5
40	1344518.5
41	1622882.5
42	1894202.0
43	2144612.0
44	2361991.0
45	2492154.5
46	2550292.0
47	2542596.0
48	2425223.0
49	2260788.5
50	2073474.0
51	1835484.0
52	1637156.0
53	1454493.5
54	1247448.5
55	1065877.5
56	896905.0
57	726593.0
58	568225.5
59	443329.0
60	350291.5
61	296983.0
62	256966.0
63	213489.5
64	176512.5
65	148968.5
66	130245.0
67	106583.5
68	75481.5
69	52121.5
70	38558.0
71	27630.5
72	20509.0
73	17588.0
74	14378.0
75	9703.5
76	5625.0
77	1926.0
78	305.5
79	88.0
80	34.5
81	13.5
82	8.0
83	3.5
84	3.0
85	3.0
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.5
96	0.5
97	0.0
98	0.5
99	1.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02437987104519677
2	0.0
3	0.002328131546688902
4	0.0
5	0.0
6	0.0
7	0.0
8	9.272212220579175E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0024100192904847775
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	6.034496812034546E-4
26-27	0.0
28-29	6.903766707713843E-4
30-31	0.0
32-33	1.0078491544107801E-5
34-35	9.322604678299715E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	3.275509751835035E-5
74-75	6.412440244938588E-4
76-77	0.0
78-79	0.0016327156301454637
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0017347603570295551
88-89	4.283358906245815E-5
90-91	0.0
92-93	3.943209816632177E-4
94-95	0.0016112988356142344
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.9688479E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.954573332986627
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.51190474499334	17.195390520456044
2	15.35515225381896	8.58493459437114
3	6.833245377627689	5.730613770335554
4	3.845799706192262	4.3003075964292
5	2.435186057722832	3.403729361503976
6	1.6933466962185346	2.8402070638566994
7	1.2688372339377605	2.4828862452615925
8	0.9418815134529755	2.106391667104451
9	0.7501918690348189	1.8874164255069732
>10	4.737547810730857	24.802808329054685
>50	0.38238273544427204	7.366254993096281
>100	0.2192118534658409	11.450203394362887
>500	0.016458804385415752	3.1315875558802464
>1k	0.008634062607184528	4.3180474615146585
>5k	2.097464382000188E-4	0.3687714634256037
>10k+	9.533929009091765E-6	0.030449557839929482
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.283358906245815E-5	0.0	2.51962288602695E-6	2.51962288602695E-6	0.0
2	6.04709492646468E-5	2.51962288602695E-6	2.51962288602695E-6	2.51962288602695E-6	0.0
3	7.55886865808085E-5	2.51962288602695E-6	2.51962288602695E-6	2.51962288602695E-6	1.51177373161617E-5
4	1.4865775027559007E-4	2.51962288602695E-6	2.51962288602695E-6	2.51962288602695E-6	1.763736020218865E-5
5	2.01569830882156E-4	2.51962288602695E-6	2.51962288602695E-6	2.51962288602695E-6	1.763736020218865E-5
6	2.3432492840050635E-4	2.51962288602695E-6	1.763736020218865E-5	2.51962288602695E-6	1.763736020218865E-5
7	2.6959964880488366E-4	2.51962288602695E-6	1.763736020218865E-5	7.55886865808085E-6	1.763736020218865E-5
8	2.9479587766515316E-4	7.55886865808085E-6	1.763736020218865E-5	7.55886865808085E-6	1.763736020218865E-5
9	3.225117294114496E-4	7.55886865808085E-6	1.763736020218865E-5	7.55886865808085E-6	2.51962288602695E-5
10-11	3.6786494135993475E-4	1.763736020218865E-5	3.275509751835035E-5	8.818680101094325E-6	2.6456040303282974E-5
12-13	4.182573990804737E-4	2.771585174629645E-5	4.03139661764312E-5	1.1338302987121275E-5	3.149528607533688E-5
14-15	4.888068398892283E-4	3.0235474632323398E-5	4.03139661764312E-5	1.259811443013475E-5	3.52747204043773E-5
16-17	5.656553379130503E-4	4.283358906245815E-5	4.03139661764312E-5	1.259811443013475E-5	3.52747204043773E-5
18-19	6.273860986207105E-4	4.53532119484851E-5	4.03139661764312E-5	1.259811443013475E-5	3.52747204043773E-5
20-21	6.941561051004247E-4	4.53532119484851E-5	4.03139661764312E-5	1.259811443013475E-5	3.52747204043773E-5
22-23	7.848625289973949E-4	4.53532119484851E-5	4.03139661764312E-5	1.51177373161617E-5	3.779434329040425E-5
24-25	8.919465016535403E-4	5.1652269163552476E-5	4.03139661764312E-5	1.51177373161617E-5	3.779434329040425E-5
26-27	0.0010305257603850225	7.180925225176807E-5	4.03139661764312E-5	1.51177373161617E-5	3.779434329040425E-5
28-29	0.0011842227564326665	7.684849802382198E-5	4.03139661764312E-5	1.51177373161617E-5	4.283358906245815E-5
30-31	0.0014626410853386443	7.810830946683545E-5	4.283358906245815E-5	1.51177373161617E-5	4.9132646277525524E-5
32-33	0.0018153882893824176	7.936812090984892E-5	4.53532119484851E-5	1.51177373161617E-5	5.291208060656595E-5
34-35	0.002285297957626444	8.56671781249163E-5	4.53532119484851E-5	1.763736020218865E-5	5.54317034925929E-5
36-37	0.002854732729868534	8.818680101094326E-5	4.53532119484851E-5	1.763736020218865E-5	6.299057215067375E-5
38-39	0.0037277820598768724	9.07064238969702E-5	4.53532119484851E-5	1.763736020218865E-5	7.306906369478156E-5
40-41	0.004935941233726795	9.07064238969702E-5	4.53532119484851E-5	1.763736020218865E-5	7.810830946683545E-5
42-43	0.0065107055374936385	1.0456434977011844E-4	4.53532119484851E-5	1.763736020218865E-5	7.810830946683545E-5
44-45	0.008282000426370586	1.2220170997230708E-4	4.787283483451205E-5	1.8897171645202126E-5	8.06279323528624E-5
46-47	0.010370767798886927	1.3983907017449572E-4	4.787283483451205E-5	2.1416794531229074E-5	8.314755523888935E-5
48-49	0.013016371829215223	1.410988816175092E-4	4.787283483451205E-5	2.267660597424255E-5	8.944661245395673E-5
50-51	0.016591716704487468	1.423586930605227E-4	4.787283483451205E-5	2.267660597424255E-5	9.07064238969702E-5
52-53	0.021140895825209123	1.4361850450353615E-4	4.787283483451205E-5	2.267660597424255E-5	9.07064238969702E-5
54-55	0.02622171537488247	1.4613812738956312E-4	5.0392457720539E-5	2.267660597424255E-5	9.196623533998367E-5
56-57	0.0319185827201894	1.7763341346489997E-4	6.802981792272764E-5	2.267660597424255E-5	9.322604678299715E-5
58-59	0.03879337376471394	1.8393247067996737E-4	8.692698956792977E-5	2.267660597424255E-5	9.448585822601063E-5
60-61	0.04785645728575287	1.952707736670886E-4	8.818680101094326E-5	2.771585174629645E-5	9.826529255505105E-5
62-63	0.058910042886753104	2.01569830882156E-4	8.818680101094326E-5	2.771585174629645E-5	1.0330453832710495E-4
64-65	0.07273269403949695	2.01569830882156E-4	8.818680101094326E-5	2.771585174629645E-5	1.058241612131319E-4
66-67	0.08898048222004173	2.01569830882156E-4	9.07064238969702E-5	2.771585174629645E-5	1.058241612131319E-4
68-69	0.10814347407971972	2.0282964232516947E-4	9.322604678299715E-5	3.02354746323234E-5	1.0834378409915885E-4
70-71	0.13150919691329063	2.0408945376818296E-4	9.448585822601063E-5	3.02354746323234E-5	1.0834378409915885E-4
72-73	0.1614914494455683	2.066090766542099E-4	9.826529255505105E-5	3.02354746323234E-5	1.1212321842819927E-4
74-75	0.1982212520666262	2.1920719108434465E-4	9.826529255505105E-5	3.02354746323234E-5	1.1338302987121276E-4
76-77	0.24351651268873267	2.7967814034899144E-4	9.826529255505105E-5	3.02354746323234E-5	1.1338302987121276E-4
78-79	0.2989091620265921	2.847173861210454E-4	9.826529255505105E-5	3.02354746323234E-5	1.1716246420025317E-4
80-81	0.3654990154699554	2.847173861210454E-4	9.826529255505105E-5	3.02354746323234E-5	1.209418985292936E-4
82-83	0.44761100570268764	2.847173861210454E-4	9.952510399806452E-5	3.02354746323234E-5	1.310203900734014E-4
84-85	0.5504683109675228	2.935360662221397E-4	1.00784915441078E-4	3.02354746323234E-5	1.310203900734014E-4
86-87	0.6779561897547145	3.0487436920926094E-4	1.00784915441078E-4	3.02354746323234E-5	1.3228020151641486E-4
88-89	0.8320374282924775	3.325902209555574E-4	1.00784915441078E-4	3.02354746323234E-5	1.3354001295942834E-4
90	0.965595078612108	3.52747204043773E-4	1.058241612131319E-4	3.02354746323234E-5	1.3354001295942834E-4
>>END_MODULE
