##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654141_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24414141
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50490578390614	32.0	32.0	32.0	32.0	32.0
2	31.421031032793657	32.0	32.0	32.0	32.0	32.0
3	35.25285128811208	37.0	37.0	37.0	32.0	37.0
4	35.9799759901444	37.0	37.0	37.0	32.0	37.0
5	36.22481225122768	37.0	37.0	37.0	37.0	37.0
6	39.31761785106426	41.0	41.0	41.0	37.0	41.0
7	39.5041889862109	41.0	41.0	41.0	37.0	41.0
8	39.68945890826141	41.0	41.0	41.0	37.0	41.0
9	39.87317608266455	41.0	41.0	41.0	37.0	41.0
10-11	39.87273254873067	41.0	41.0	41.0	37.0	41.0
12-13	39.79614636042284	41.0	41.0	41.0	37.0	41.0
14-15	39.730583967709535	41.0	41.0	41.0	37.0	41.0
16-17	39.75226793766777	41.0	41.0	41.0	37.0	41.0
18-19	39.71797109306446	41.0	41.0	41.0	37.0	41.0
20-21	39.703307173494245	41.0	41.0	41.0	37.0	41.0
22-23	39.70655781008228	41.0	41.0	41.0	37.0	41.0
24-25	39.65709399728624	41.0	41.0	41.0	37.0	41.0
26-27	39.494517357788666	41.0	41.0	41.0	37.0	41.0
28-29	39.572174994811405	41.0	41.0	41.0	37.0	41.0
30-31	39.634106008480906	41.0	41.0	41.0	37.0	41.0
32-33	39.62632129879155	41.0	41.0	41.0	37.0	41.0
34-35	39.48170404602808	41.0	41.0	41.0	37.0	41.0
36-37	39.39982240210704	41.0	41.0	41.0	37.0	41.0
38-39	39.35191100927942	41.0	41.0	41.0	37.0	41.0
40-41	39.35483331156317	41.0	41.0	41.0	37.0	41.0
42-43	39.31743541581086	41.0	41.0	41.0	37.0	41.0
44-45	39.31033453112276	41.0	41.0	41.0	37.0	41.0
46-47	39.26339460397153	41.0	41.0	41.0	37.0	41.0
48-49	39.234706476054185	41.0	41.0	41.0	37.0	41.0
50-51	39.23800888591575	41.0	41.0	41.0	37.0	41.0
52-53	39.252549782521534	41.0	41.0	41.0	37.0	41.0
54-55	39.24532472799268	41.0	41.0	41.0	37.0	41.0
56-57	39.205078114360035	41.0	41.0	41.0	37.0	41.0
58-59	39.1787157287246	41.0	41.0	41.0	37.0	41.0
60-61	39.13473085127181	41.0	41.0	41.0	37.0	41.0
62-63	39.07504107967591	41.0	41.0	41.0	37.0	41.0
64-65	39.094989395694896	41.0	41.0	41.0	37.0	41.0
66-67	39.085355286512026	41.0	41.0	41.0	37.0	41.0
68-69	39.03477202003543	41.0	41.0	41.0	37.0	41.0
70-71	38.961029552504016	41.0	41.0	41.0	37.0	41.0
72-73	38.97294803450181	41.0	41.0	41.0	37.0	41.0
74-75	38.87348170472187	41.0	41.0	41.0	34.5	41.0
76-77	37.95029372526356	41.0	39.0	41.0	32.0	41.0
78-79	38.48905210304143	41.0	39.0	41.0	32.0	41.0
80-81	38.96466728032741	41.0	41.0	41.0	34.5	41.0
82-83	39.041624790321315	41.0	41.0	41.0	37.0	41.0
84-85	39.008539620542045	41.0	41.0	41.0	37.0	41.0
86-87	38.96045996457545	41.0	41.0	41.0	37.0	41.0
88-89	38.88653819522055	41.0	41.0	41.0	32.0	41.0
90-91	38.77017573544775	41.0	41.0	41.0	32.0	41.0
92-93	38.6910795673704	41.0	41.0	41.0	32.0	41.0
94-95	38.560852130738496	41.0	41.0	41.0	32.0	41.0
96-97	38.58916744193458	41.0	41.0	41.0	32.0	41.0
98-99	38.53971206687141	41.0	39.0	41.0	32.0	41.0
100-101	37.49899756866318	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	19.0
13	83.0
14	239.0
15	871.0
16	3253.0
17	8704.0
18	15466.0
19	22727.0
20	29355.0
21	36991.0
22	45835.0
23	56640.0
24	68121.0
25	81051.0
26	96093.0
27	113663.0
28	133747.0
29	157672.0
30	186119.0
31	221294.0
32	263070.0
33	317629.0
34	391672.0
35	497780.0
36	655342.0
37	938444.0
38	1573873.0
39	4068115.0
40	1.4430273E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	29.857590509420568	20.985297352835264	12.32017432278474	36.836937814959434
2	26.855326592476857	25.588107237350876	31.07711494641643	16.479451223755838
3	20.511426291468187	28.353247760973453	28.7606745288904	22.37465141866796
4	24.343156548126277	30.897730015222578	23.271829149267305	21.487284287383837
5	28.04475402179907	32.527176742398666	21.76009215012619	17.667977085676075
6	22.332787945851194	37.92885468423949	21.425961959683924	18.312395410225392
7	22.076365496537438	22.41931837782046	35.3806795823781	20.123636543264002
8	22.590737110350435	24.511751197858093	27.719020081407237	25.178491610384235
9	23.839814802413077	23.019810527022024	29.452930578225136	23.687444092339764
10-11	24.842547194267453	30.64159414824384	23.096954342976883	21.418904314511824
12-13	25.288046992930546	25.736169246773184	25.8374259273793	23.138357832916974
14-15	24.552584094603205	27.46679885235364	25.71602457772321	22.26459247531994
16-17	25.510901723747715	26.85826500621972	25.316225488803628	22.314607781228936
18-19	25.044557168732663	27.436416870042653	25.350633880585843	22.168392080638842
20-21	25.359341372450384	27.354881561802895	25.211782842504444	22.07399422324227
22-23	25.232984850869826	27.19319717208154	25.382363442563882	22.19145453448475
24-25	24.719470913059176	27.291612010828974	25.779526901116917	22.209390174994937
26-27	25.021097040364243	27.367613473178366	25.52538404621562	22.08590544024177
28-29	25.243711634039208	27.05803097203584	25.442461165760776	22.255796228164176
30-31	24.786042092620328	27.258300990499766	25.666867486497907	22.288789430381996
32-33	25.031649860059858	27.378470394662735	25.41203595804212	22.17784378723528
34-35	25.192850323916783	27.10534235056642	25.329005022130413	22.372802303386386
36-37	24.853749718247307	27.257545125179703	25.61521824585186	22.273486910721125
38-39	25.0511844487668	27.430913922245843	25.307177861046792	22.210723767940564
40-41	25.287637603141555	26.984195348097646	25.347811745660024	22.38035530310077
42-43	24.874958615132385	27.153212217341306	25.632489596080827	22.33933957144549
44-45	25.056741302566614	27.246839560494294	25.469723325105765	22.226695811833324
46-47	25.22307870672165	27.040316511647898	25.38360452657335	22.3530002550571
48-49	24.891268723291045	27.170595529522128	25.62545354832617	22.312682198860653
50-51	25.17018631077661	27.197395722228286	25.31924730210317	22.313170664891935
52-53	25.289684577287886	27.029510319666183	25.295513207848884	22.385291895197046
54-55	24.913218186768777	27.160055881968336	25.612496458224765	22.314229473038118
56-57	24.98420604804569	27.326495462719425	25.46848153929981	22.22081694993508
58-59	25.239036628529583	26.905835152662583	25.41397936678802	22.441148852019815
60-61	24.9583939897783	27.13842358819833	25.538152253646768	22.3650301683766
62-63	25.095371662339993	27.175066210864152	25.40127126169955	22.328290865096307
64-65	25.242965259658867	27.02741077675135	25.384168276099523	22.34545568749026
66-67	24.890988458420697	27.17933788191519	25.69814737519902	22.23152628446509
68-69	25.184857626501188	27.30413447432072	25.37806802219983	22.13293987697826
70-71	25.41600915797682	27.103648928493286	25.18288387632635	22.297458037203544
72-73	24.925281387116605	27.230857089843276	25.616713867879	22.227147655161122
74-75	25.038474880438383	27.375267065211528	25.38463514000441	22.201622914345677
76-77	25.33897683048021	27.07478207842383	25.31872825074799	22.267512840347965
78-79	24.917115410672242	27.202623503792978	25.66457994628343	22.215681139251352
80-81	25.154230460316995	27.335347270161765	25.346198427821815	22.164223841699428
82-83	25.344356910763093	27.116861731487447	25.268171545079277	22.270609812670184
84-85	25.03498249313739	27.317974043374093	25.558217166197384	22.088826297291135
86-87	25.222966067083824	27.48898476501795	25.3456183447126	21.942430823185628
88-89	25.365082474128414	27.30335054589879	25.207061350223213	22.124505629749578
90-91	25.07500468683293	27.449945914541907	25.461538867986384	22.01351053063878
92-93	25.415129289209887	27.54765977635666	25.13406881692049	21.90314211751296
94-95	25.700636356974794	27.29624942149273	25.043225997800374	21.959888223732104
96-97	25.299844539428218	27.443643878651663	25.30983923793701	21.946672343983114
98-99	25.50176407002852	27.66059306168022	25.05700948653175	21.780633381759507
100-101	25.90236258491124	27.354523969872723	24.95023966145357	21.792873783762463
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	34.5
2	24.5
3	18.5
4	17.5
5	25.0
6	36.0
7	52.0
8	71.5
9	78.0
10	98.0
11	144.0
12	173.5
13	222.5
14	291.5
15	346.0
16	415.5
17	547.5
18	760.5
19	957.5
20	1206.0
21	1618.5
22	2167.5
23	2900.5
24	3839.5
25	5049.0
26	6785.0
27	9222.0
28	12500.5
29	16960.0
30	22891.0
31	31157.5
32	43254.5
33	60798.0
34	89804.0
35	136305.0
36	200174.0
37	289782.0
38	419121.0
39	586316.5
40	781450.5
41	989723.0
42	1200860.0
43	1387822.0
44	1529220.5
45	1619905.0
46	1656824.0
47	1647762.5
48	1588905.0
49	1471424.5
50	1311814.0
51	1167657.0
52	1039016.5
53	892966.0
54	752906.0
55	628477.0
56	500170.5
57	391392.0
58	315730.5
59	259896.0
60	215966.0
61	179705.5
62	149525.0
63	124960.5
64	112616.0
65	100604.0
66	88122.5
67	77302.0
68	63475.0
69	50444.0
70	44117.0
71	38541.5
72	29647.5
73	21227.0
74	13950.5
75	9044.5
76	5244.5
77	2853.5
78	1722.0
79	1041.0
80	562.0
81	390.0
82	317.5
83	249.0
84	194.5
85	163.5
86	155.0
87	142.0
88	111.0
89	86.5
90	77.0
91	67.0
92	62.0
93	59.5
94	54.5
95	48.5
96	47.0
97	50.5
98	51.0
99	103.0
100	504.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017412040014023022
2	0.0070205214264962255
3	7.49565589876785E-4
4	0.0016752586134404649
5	2.7443111760516167E-4
6	0.0012533719699579027
7	0.0
8	0.024539057098097367
9	0.0
10-11	0.0
12-13	0.003837939659642336
14-15	0.0
16-17	0.0011161564111553217
18-19	0.0
20-21	4.0959868299277866E-4
22-23	0.0
24-25	2.4575920979566723E-5
26-27	0.005556206134797043
28-29	0.0010117087469921633
30-31	2.1094332174128103E-4
32-33	5.365742747205401E-4
34-35	0.0
36-37	0.0
38-39	9.482209511282826E-4
40-41	0.0
42-43	9.830368391826689E-5
44-45	0.001996793579589796
46-47	0.0
48-49	0.001628154764896295
50-51	0.001265659930447686
52-53	7.086057215775071E-4
54-55	7.516135832917488E-4
56-57	0.008628196257242883
58-59	6.246379915639874E-4
60-61	0.0
62-63	0.005033967813981249
64-65	1.0239967074819467E-5
66-67	0.0027484071628815446
68-69	3.358709200540785E-4
70-71	6.14398024489168E-5
72-73	1.7817542710185872E-4
74-75	6.553578927884459E-4
76-77	3.399669068840063E-4
78-79	2.990070385847284E-4
80-81	0.0017715143039437678
82-83	0.005390318668184967
84-85	5.877741100946374E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	5.734381561898901E-5
96-97	0.004909040215668452
98-99	1.0239967074819467E-5
100-101	4.8742243276140657E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.4414141E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.89493779484457
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.29359667113354	19.7585783300583
2	17.470754352917133	11.843402640422726
3	8.162902140436437	8.300431808264896
4	4.448636671090513	6.031450529539095
5	2.733790916404398	4.63308365278191
6	1.7803264683533777	3.620643293961183
7	1.279052032677092	3.034737235877168
8	0.942029184470005	2.5544016486831187
9	0.7203684453223271	2.1975159279212253
>10	3.8088875303746583	23.159641029969176
>50	0.2381631741847154	5.510130694552959
>100	0.11344796570233392	6.898198918465481
>500	0.006198378955854553	1.4270570787534185
>1k	0.0018206143347498098	0.9488263874914962
>5k	1.2726821399000007E-5	0.02366090885753933
>10k+	1.2726821399000007E-5	0.05823991440029872
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6383947319711145E-5	4.095986829927786E-6	8.191973659855573E-6	0.0	4.095986829927786E-6
2	1.6383947319711145E-5	4.095986829927786E-6	1.228796048978336E-5	4.095986829927786E-6	4.095986829927786E-6
3	3.6863881469350076E-5	4.095986829927786E-6	1.228796048978336E-5	4.095986829927786E-6	4.095986829927786E-6
4	4.095986829927787E-5	4.095986829927786E-6	1.6383947319711145E-5	1.228796048978336E-5	4.095986829927786E-6
5	6.14398024489168E-5	4.095986829927786E-6	2.457592097956672E-5	1.228796048978336E-5	4.095986829927786E-6
6	1.0239967074819466E-4	4.095986829927786E-6	2.8671907809494505E-5	1.228796048978336E-5	4.095986829927786E-6
7	1.1059164440805024E-4	1.228796048978336E-5	3.276789463942229E-5	1.228796048978336E-5	4.095986829927786E-6
8	1.269755917277614E-4	1.228796048978336E-5	4.095986829927787E-5	1.6383947319711145E-5	4.095986829927786E-6
9	1.3107157855768916E-4	1.228796048978336E-5	4.095986829927787E-5	1.6383947319711145E-5	4.095986829927786E-6
10-11	1.6383947319711147E-4	1.843194073467504E-5	5.734381561898901E-5	1.6383947319711145E-5	4.095986829927786E-6
12-13	2.10943321741281E-4	2.457592097956672E-5	6.758378269380847E-5	1.6383947319711145E-5	1.6383947319711145E-5
14-15	2.949110517548006E-4	2.457592097956672E-5	8.396773001351964E-5	1.6383947319711145E-5	2.457592097956672E-5
16-17	3.624948344486091E-4	3.891187488431397E-5	1.0649565757812245E-4	1.6383947319711145E-5	2.457592097956672E-5
18-19	4.812784525165149E-4	4.710384854416955E-5	1.249275983127975E-4	1.6383947319711145E-5	2.6623914394530612E-5
20-21	6.451179257136264E-4	4.915184195913344E-5	1.5564749953725588E-4	1.6383947319711145E-5	2.8671907809494505E-5
22-23	8.335333198903046E-4	4.915184195913344E-5	2.0070335466646153E-4	1.843194073467504E-5	2.8671907809494505E-5
24-25	0.001128444371645105	5.119983537409733E-5	2.088953283263171E-4	2.0479934149638934E-5	2.8671907809494505E-5
26-27	0.0016138188109915477	5.3247828789061225E-5	2.129913151562449E-4	2.0479934149638934E-5	2.8671907809494505E-5
28-29	0.0021381051252223043	5.3247828789061225E-5	2.375672361358116E-4	2.0479934149638934E-5	3.07199012244584E-5
30-31	0.0027340712089767975	5.3247828789061225E-5	2.4166322296573938E-4	2.0479934149638934E-5	3.276789463942229E-5
32-33	0.003432436963479485	5.3247828789061225E-5	2.539511834555228E-4	2.2527927564602827E-5	3.276789463942229E-5
34-35	0.00408779485626793	5.3247828789061225E-5	2.744311176051617E-4	2.457592097956672E-5	3.276789463942229E-5
36-37	0.0048803683078589575	5.3247828789061225E-5	3.031030254146562E-4	2.6623914394530612E-5	3.4815888054386183E-5
38-39	0.005928940936320471	5.3247828789061225E-5	3.1948697273436735E-4	3.276789463942229E-5	3.6863881469350076E-5
40-41	0.007219176787747724	6.14398024489168E-5	3.481588805438619E-4	4.095986829927787E-5	3.6863881469350076E-5
42-43	0.008785891750195101	6.14398024489168E-5	3.7887878176832025E-4	4.095986829927787E-5	3.6863881469350076E-5
44-45	0.010545118093649088	6.14398024489168E-5	3.850227620132119E-4	4.095986829927787E-5	3.6863881469350076E-5
46-47	0.012570583581048376	6.553578927884458E-5	4.4031858421723707E-4	4.915184195913344E-5	3.6863881469350076E-5
48-49	0.014970831863386061	6.553578927884458E-5	4.5055855129205654E-4	4.915184195913344E-5	4.095986829927787E-5
50-51	0.018083781854131176	6.553578927884458E-5	4.587505249519121E-4	5.119983537409733E-5	4.300786171424176E-5
52-53	0.021839801777174955	6.553578927884458E-5	4.915184195913344E-4	5.3247828789061225E-5	4.710384854416955E-5
54-55	0.02616925985640863	6.553578927884458E-5	5.18142333985865E-4	5.3247828789061225E-5	5.734381561898901E-5
56-57	0.03105782013792744	6.553578927884458E-5	5.406702615504677E-4	5.529582220402512E-5	6.553578927884458E-5
58-59	0.036921225284969064	6.553578927884458E-5	5.570542088701789E-4	5.734381561898901E-5	6.553578927884458E-5
60-61	0.04505585512920565	6.553578927884458E-5	6.246379915639874E-4	5.734381561898901E-5	6.553578927884458E-5
62-63	0.05558254128212006	6.553578927884458E-5	7.434216096318933E-4	5.734381561898901E-5	6.553578927884458E-5
64-65	0.0685074277239572	6.553578927884458E-5	8.212453594005212E-4	6.14398024489168E-5	6.758378269380847E-5
66-67	0.08462923188655297	7.167976952373627E-5	8.294373330603767E-4	6.14398024489168E-5	7.167976952373627E-5
68-69	0.10391928186209787	7.372776293870015E-5	8.499172672100158E-4	6.14398024489168E-5	7.372776293870015E-5
70-71	0.12913212879371835	7.782374976862794E-5	8.642532211147629E-4	6.14398024489168E-5	7.372776293870015E-5
72-73	0.16162764030895044	7.782374976862794E-5	9.236450301487158E-4	6.14398024489168E-5	7.987174318359184E-5
74-75	0.20514954837034816	8.191973659855574E-5	9.297890103936075E-4	6.14398024489168E-5	8.191973659855574E-5
76-77	0.26061535402781527	8.191973659855574E-5	9.40028977468427E-4	6.14398024489168E-5	8.191973659855574E-5
78-79	0.32958358027013934	8.806371684344741E-5	9.420769708833909E-4	6.34877958638807E-5	8.191973659855574E-5
80-81	0.41532692057443266	9.42076970883391E-5	9.420769708833909E-4	6.553578927884458E-5	8.191973659855574E-5
82-83	0.5240651309419406	9.42076970883391E-5	9.625569050330299E-4	6.553578927884458E-5	8.191973659855574E-5
84-85	0.6644694154916202	9.42076970883391E-5	9.748448655228132E-4	6.553578927884458E-5	8.396773001351964E-5
86-87	0.8439698943329605	1.0035167733323078E-4	9.789408523527409E-4	6.553578927884458E-5	8.806371684344741E-5
88-89	1.0625071756569278	1.0239967074819466E-4	9.95324799672452E-4	6.553578927884458E-5	9.011171025841131E-5
90	1.2530033311432092	1.1468763123797802E-4	9.95324799672452E-4	6.553578927884458E-5	9.011171025841131E-5
>>END_MODULE
