##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654130_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30435079
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.673518672318874	32.0	32.0	32.0	32.0	32.0
2	31.69720729162556	32.0	32.0	32.0	32.0	32.0
3	35.83234950696202	37.0	37.0	37.0	32.0	37.0
4	36.391527782793005	37.0	37.0	37.0	37.0	37.0
5	36.56298914157575	37.0	37.0	37.0	37.0	37.0
6	40.18874831900387	41.0	41.0	41.0	37.0	41.0
7	40.22251935012227	41.0	41.0	41.0	37.0	41.0
8	40.25671916277924	41.0	41.0	41.0	41.0	41.0
9	40.30217680722958	41.0	41.0	41.0	41.0	41.0
10-11	40.3315781437597	41.0	41.0	41.0	41.0	41.0
12-13	40.31089833872289	41.0	41.0	41.0	41.0	41.0
14-15	40.305572116306976	41.0	41.0	41.0	41.0	41.0
16-17	40.30326321807806	41.0	41.0	41.0	41.0	41.0
18-19	40.30201850305695	41.0	41.0	41.0	41.0	41.0
20-21	40.27200156109337	41.0	41.0	41.0	41.0	41.0
22-23	40.2768810292886	41.0	41.0	41.0	41.0	41.0
24-25	40.26421263766064	41.0	41.0	41.0	41.0	41.0
26-27	40.185194245758325	41.0	41.0	41.0	41.0	41.0
28-29	40.196072712674734	41.0	41.0	41.0	41.0	41.0
30-31	40.11115098469105	41.0	41.0	41.0	41.0	41.0
32-33	40.12922169513672	41.0	41.0	41.0	41.0	41.0
34-35	40.148521480755804	41.0	41.0	41.0	41.0	41.0
36-37	40.13675114823917	41.0	41.0	41.0	41.0	41.0
38-39	40.06449708903335	41.0	41.0	41.0	37.0	41.0
40-41	40.08576340478696	41.0	41.0	41.0	37.0	41.0
42-43	40.072395212116916	41.0	41.0	41.0	37.0	41.0
44-45	40.06688049010815	41.0	41.0	41.0	37.0	41.0
46-47	40.05437107621768	41.0	41.0	41.0	37.0	41.0
48-49	39.964006122671805	41.0	41.0	41.0	37.0	41.0
50-51	39.92440392548349	41.0	41.0	41.0	37.0	41.0
52-53	39.96711896164291	41.0	41.0	41.0	37.0	41.0
54-55	39.9353124071076	41.0	41.0	41.0	37.0	41.0
56-57	39.92592991133685	41.0	41.0	41.0	37.0	41.0
58-59	39.92449618744213	41.0	41.0	41.0	37.0	41.0
60-61	39.9083591503081	41.0	41.0	41.0	37.0	41.0
62-63	39.86116093209418	41.0	41.0	41.0	37.0	41.0
64-65	39.806021006221144	41.0	41.0	41.0	37.0	41.0
66-67	39.780475795709286	41.0	41.0	41.0	37.0	41.0
68-69	39.75665077130242	41.0	41.0	41.0	37.0	41.0
70-71	39.73335217891171	41.0	41.0	41.0	37.0	41.0
72-73	39.70742055573439	41.0	41.0	41.0	37.0	41.0
74-75	39.68557998485892	41.0	41.0	41.0	37.0	41.0
76-77	39.12656891739956	41.0	39.0	41.0	34.5	41.0
78-79	39.46642469697549	41.0	41.0	41.0	37.0	41.0
80-81	39.65854271316332	41.0	41.0	41.0	37.0	41.0
82-83	39.73059789002026	41.0	41.0	41.0	37.0	41.0
84-85	39.76784440086388	41.0	41.0	41.0	37.0	41.0
86-87	39.77156495963096	41.0	41.0	41.0	37.0	41.0
88-89	39.65239779729174	41.0	41.0	41.0	37.0	41.0
90-91	39.671670886742234	41.0	41.0	41.0	37.0	41.0
92-93	39.62083259254888	41.0	41.0	41.0	37.0	41.0
94-95	39.61234639804944	41.0	41.0	41.0	37.0	41.0
96-97	39.574584412940084	41.0	41.0	41.0	37.0	41.0
98-99	39.52724072114286	41.0	41.0	41.0	37.0	41.0
100-101	38.99828339528871	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	2.0
16	9.0
17	20.0
18	55.0
19	189.0
20	545.0
21	1425.0
22	3507.0
23	7622.0
24	14483.0
25	25344.0
26	41553.0
27	65119.0
28	94209.0
29	129017.0
30	168395.0
31	212903.0
32	262187.0
33	316861.0
34	378967.0
35	463771.0
36	587236.0
37	796567.0
38	1237061.0
39	2929123.0
40	2.2698908E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.77930994486848	11.138257566853534	8.45125817049816	44.63117431777982
2	22.24847190309577	13.703358548863962	35.51373400410756	28.534435543932712
3	19.680796413110286	17.261481241091072	24.346849467254195	38.71087287854444
4	22.72761309408791	25.44041038960339	22.720447021018085	29.11152949529062
5	23.54868209804877	30.17542684873596	24.67518484180705	21.600706211408223
6	20.17504538102234	32.63989884829936	25.04638151259604	22.13867425808226
7	16.1679816898126	23.735660420004166	40.529732155451285	19.56662573473195
8	19.34672159056988	24.042181720638872	30.478107186776153	26.132989502015093
9	18.917913766545507	22.71390194190066	33.20212837298697	25.166055918566858
10-11	21.99980808226657	30.422317091696872	23.804506702178948	23.773368123857612
12-13	21.98347834089736	24.061205821085597	27.1852029692448	26.77011286877225
14-15	20.990246156417076	25.733144967358225	27.73693473902269	25.539674137202013
16-17	21.672437389155995	26.15912237440834	26.883040338477205	25.28539989795846
18-19	21.510407040443813	26.526082535758416	26.644228274903647	25.319282148894125
20-21	21.703585852364636	26.365201812027493	26.777922935570498	25.15328940003737
22-23	21.731358081902794	26.6104089954884	26.703628730518492	24.95460419209032
24-25	21.711732519582572	26.267109167656464	26.520793774004304	25.50036453875666
26-27	21.530486252393167	26.381727151094303	26.784203517263748	25.30358307924878
28-29	21.67458184706366	26.533256456871975	26.631587400246765	25.160574295817607
30-31	21.692271293844417	26.30948940504389	26.607956551158686	25.39028274995301
32-33	21.717817785194512	26.243452497691887	26.741281006696255	25.297448710417342
34-35	21.831306894258432	26.406331982906963	26.486408988785605	25.275952134049
36-37	21.713804324279888	26.21739210862571	26.436593116778173	25.63221045031623
38-39	21.82529346681172	26.313178490599604	26.641639661129812	25.219888381458865
40-41	21.99719442160804	26.464132062873897	26.50793185061225	25.030741664905815
42-43	21.92032095596006	26.142621479641964	26.42348981581418	25.5135677485838
44-45	21.931456133233628	26.112391559752478	26.633070346227782	25.323081960786105
46-47	21.94272225964519	26.287331517382107	26.501277125129345	25.268669097843354
48-49	21.88747891865173	26.111476497235316	26.452050609101423	25.54899397501153
50-51	21.944700051853836	26.12810021677476	26.55536248390832	25.371837247463088
52-53	22.26291488179934	26.246796093769724	26.34939699279957	25.140892031631363
54-55	22.118968378560805	26.032201197834908	26.321701021377343	25.52712940222695
56-57	22.001790612543623	25.950164049673802	26.6981769394424	25.34986839834017
58-59	22.076276930128007	26.08914900359321	26.513986701740063	25.32058736453872
60-61	21.965569742116013	25.921514783489414	26.461786028042816	25.65112944635176
62-63	22.12654594925447	25.917511739810394	26.621787003584014	25.334155307351118
64-65	22.265141628647093	26.11205252471851	26.39944575933854	25.22336008729586
66-67	22.103356025463103	25.900725226181194	26.43080625419976	25.565112494155944
68-69	22.1160279799357	25.86861973693929	26.63179820359171	25.383554079533294
70-71	22.305579645302114	26.139941702265716	26.411211743713658	25.143266908718516
72-73	22.249636677466814	25.95345982180628	26.326075907343625	25.470827593383277
74-75	22.15031214474587	25.922530051589483	26.576607538952008	25.350550264712634
76-77	22.201132855649405	26.070148585652152	26.43977846521523	25.288940093483212
78-79	22.18067567828646	25.863357462948343	26.39199422403282	25.563972634732373
80-81	22.230978176136716	25.934690824359397	26.55931852929283	25.275012470211056
82-83	22.354581331644034	26.12880490729171	26.283867991513638	25.232745769550625
84-85	22.366525040584754	25.910962195193754	26.29602946232557	25.42648330189592
86-87	22.377553124777034	25.9328012319606	26.46358119805823	25.226064445204138
88-89	22.56044579348718	26.126127354556893	26.305734248299473	25.007692603656455
90-91	22.46719258418837	25.925557008953348	26.23575338851426	25.371497018344023
92-93	22.472957279212242	25.95440929872351	26.29087704616258	25.281756375901665
94-95	22.67800914858805	26.13357435346233	26.082787562338837	25.10562893561078
96-97	22.609466818119493	26.137589161741058	25.878366219101895	25.37457780103755
98-99	22.671234520698395	26.240949294924388	26.08260379771264	25.005212386664578
100-101	22.724555766719053	26.40019761407552	25.899328206113744	24.975918413091684
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38.0
1	31.5
2	25.0
3	31.5
4	34.5
5	42.0
6	56.0
7	72.5
8	87.5
9	93.0
10	108.5
11	148.0
12	181.5
13	201.0
14	233.5
15	302.0
16	378.5
17	474.5
18	595.0
19	786.0
20	1072.5
21	1458.5
22	2083.0
23	3173.0
24	4971.5
25	7363.5
26	10713.5
27	15856.0
28	23465.0
29	34320.0
30	48997.0
31	67733.0
32	93897.5
33	128727.0
34	173137.5
35	232430.0
36	303808.5
37	391671.0
38	511162.0
39	670227.5
40	861113.0
41	1077427.0
42	1309247.0
43	1533478.0
44	1730671.5
45	1874560.5
46	1956682.0
47	1970674.5
48	1911105.0
49	1794135.0
50	1647395.5
51	1488737.0
52	1328206.5
53	1173577.0
54	1013091.5
55	849544.0
56	700107.0
57	581013.0
58	488130.5
59	404149.5
60	334737.5
61	290030.5
62	252265.0
63	210803.0
64	183782.0
65	164147.5
66	140385.5
67	115962.0
68	83665.0
69	57498.5
70	46785.0
71	37800.0
72	29295.5
73	24513.0
74	18579.5
75	11667.5
76	6864.0
77	2543.0
78	332.5
79	99.0
80	49.0
81	23.5
82	10.0
83	5.0
84	3.0
85	2.0
86	1.5
87	0.5
88	1.5
89	1.5
90	1.5
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	1.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05972056126419123
2	0.0
3	6.571364575725267E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.372090540655405E-4
12-13	0.0
14-15	0.0
16-17	0.001363558149462993
18-19	0.0020831225705049096
20-21	0.0
22-23	0.0
24-25	1.4292717952202455E-4
26-27	0.0
28-29	3.3513959336198864E-4
30-31	0.0018843387920892205
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0029176858716220186
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.002326263059806745
48-49	0.0
50-51	0.0016214842090602098
52-53	5.273520072019527E-4
54-55	0.0
56-57	7.967779548066886E-4
58-59	1.462128618098872E-4
60-61	0.0012042025585016554
62-63	0.0033743957096349247
64-65	0.0021241935991031928
66-67	9.79133321783065E-4
68-69	4.172816505585545E-4
70-71	9.101339937379496E-4
72-73	0.0
74-75	0.0
76-77	0.0010185615092374164
78-79	9.857046863587901E-6
80-81	0.0018071252583244486
82-83	0.001212416764221312
84-85	2.2999776015038437E-5
86-87	0.001115489136729364
88-89	0.0
90-91	0.002132407804822849
92-93	0.0011959883527819988
94-95	0.0
96-97	2.628545830290107E-4
98-99	0.00119763119392593
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.0435079E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.86132324743141
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.897697702664736	20.959379501817228
2	16.11451729471157	10.913177581851045
3	7.090939320925719	7.203257654213627
4	3.810033203017576	5.160510634832984
5	2.4283692667873478	4.111389835340719
6	1.6061225727223225	3.263126136596803
7	1.1857845280609383	2.810656324453203
8	0.8915846167219539	2.4152187927407454
9	0.6834509494415923	2.0828298170525095
>10	3.8862120051711933	23.990555200610945
>50	0.265173378242539	6.139499276794654
>100	0.13099663153946803	7.958884681116173
>500	0.006562668385827762	1.4837346898831971
>1k	0.0025251268397430133	1.4501309281984538
>5k	3.073476767210504E-5	0.057648944497936686
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6285458302901072E-5	0.0	9.857046863587901E-6	0.0	0.0
2	3.614250516648897E-5	0.0	1.3142729151450536E-5	0.0	6.571364575725268E-6
3	5.2570916605802144E-5	3.285682287862634E-6	1.3142729151450536E-5	0.0	6.571364575725268E-6
4	9.528478634801638E-5	3.285682287862634E-6	1.9714093727175802E-5	0.0	6.571364575725268E-6
5	1.1828456236305481E-4	3.285682287862634E-6	1.9714093727175802E-5	0.0	6.571364575725268E-6
6	1.4457002066595588E-4	6.571364575725268E-6	3.2856822878626335E-5	0.0	6.571364575725268E-6
7	1.6099843210526905E-4	1.6428411439313167E-5	3.2856822878626335E-5	0.0	6.571364575725268E-6
8	1.7085547896885696E-4	1.6428411439313167E-5	3.2856822878626335E-5	0.0	1.3142729151450536E-5
9	2.0699798413534594E-4	1.6428411439313167E-5	3.9428187454351604E-5	0.0	1.6428411439313167E-5
10-11	2.4314048930183492E-4	2.6285458302901072E-5	4.107102859828292E-5	0.0	2.4642617158969754E-5
12-13	3.1213981734695023E-4	3.614250516648897E-5	4.271386974221424E-5	0.0	5.2570916605802144E-5
14-15	4.2385301513427976E-4	3.778534631042029E-5	4.271386974221424E-5	0.0	7.392785147690926E-5
16-17	5.109235957626396E-4	4.5999552030076874E-5	4.271386974221424E-5	0.0	8.871342177229111E-5
18-19	5.947084941031367E-4	5.2570916605802144E-5	4.4356710886145557E-5	0.0	9.035626291622243E-5
20-21	6.867075981632905E-4	5.2570916605802144E-5	4.764239317400819E-5	0.0	9.199910406015375E-5
22-23	7.787067022234442E-4	5.749944003759609E-5	4.928523431793951E-5	0.0	9.528478634801638E-5
24-25	9.199910406015375E-4	7.228501033297794E-5	4.928523431793951E-5	0.0	9.69276274919477E-5
26-27	0.0010744181081310813	1.1171319778732954E-4	4.928523431793951E-5	0.0	9.857046863587902E-5
28-29	0.0012929159802739464	1.1171319778732954E-4	5.2570916605802144E-5	0.0	1.0185615092374165E-4
30-31	0.0016411983027873856	1.1335603893126086E-4	5.7499440037596096E-5	0.0	1.0349899206767297E-4
32-33	0.0020387658596187643	1.2978445037057403E-4	6.242796346939005E-5	0.0	1.0678467435553559E-4
34-35	0.002525046838222434	1.396414972341619E-4	6.242796346939005E-5	0.0	1.1171319778732954E-4
36-37	0.0031805404546510294	1.4292717952202457E-4	6.242796346939005E-5	0.0	1.2157024465091745E-4
38-39	0.003988818297465237	1.4621286180988718E-4	6.242796346939005E-5	0.0	1.2321308579484877E-4
40-41	0.005224234837701588	1.6099843210526905E-4	6.242796346939005E-5	0.0	1.248559269387801E-4
42-43	0.006855576093625386	1.9714093727175804E-4	6.242796346939005E-5	0.0	1.248559269387801E-4
44-45	0.008762914661729643	2.2671207786252172E-4	6.571364575725267E-5	0.0	1.5771274981740642E-4
46-47	0.010951179065446157	2.447833304457662E-4	6.571364575725267E-5	0.0	1.642841143931317E-4
48-49	0.013919793012530048	2.4642617158969753E-4	6.571364575725267E-5	0.0	1.7906968468851353E-4
50-51	0.01810246656497918	2.5628321845328546E-4	6.571364575725267E-5	0.0	1.938552549838954E-4
52-53	0.023857339092170582	2.759973121804612E-4	6.571364575725267E-5	0.0	2.1685503099893382E-4
54-55	0.030330133199259972	2.842115179001178E-4	7.064216918904662E-5	0.0	2.1849787214286515E-4
56-57	0.037558634232557765	3.3021106993019473E-4	1.0021330977981034E-4	0.0	2.2014071328679645E-4
58-59	0.04641354799834757	3.4006811679378256E-4	1.2157024465091745E-4	0.0	2.2014071328679645E-4
60-61	0.05792329305273037	3.5156800480130185E-4	1.2157024465091745E-4	0.0	2.2014071328679645E-4
62-63	0.07327728638391245	3.5485368708916445E-4	1.2157024465091745E-4	3.285682287862634E-6	2.2014071328679645E-4
64-65	0.09253302743193142	3.5649652823309576E-4	1.2157024465091745E-4	3.285682287862634E-6	2.2014071328679645E-4
66-67	0.11554430333497738	3.63067892808821E-4	1.2321308579484877E-4	3.285682287862634E-6	2.2014071328679645E-4
68-69	0.14361881564361967	3.745677808163402E-4	1.3142729151450534E-4	3.285682287862634E-6	2.2014071328679645E-4
70-71	0.1780445518147004	3.7785346310420287E-4	1.3142729151450534E-4	3.285682287862634E-6	2.2506923671859042E-4
72-73	0.2218114827301746	3.8278198653599684E-4	1.3142729151450534E-4	6.571364575725268E-6	2.2999776015038436E-4
74-75	0.2789478548749619	4.139959682706919E-4	1.3142729151450534E-4	6.571364575725268E-6	2.316406012943157E-4
76-77	0.35177500278543716	4.8628097860366985E-4	1.3142729151450534E-4	8.214205719656584E-6	2.431404893018349E-4
78-79	0.4406757084481364	4.928523431793951E-4	1.3471297380236797E-4	9.857046863587901E-6	2.4642617158969753E-4
80-81	0.5501332853448483	4.928523431793951E-4	1.379986560902306E-4	9.857046863587901E-6	2.546403773093541E-4
82-83	0.6845604047881721	4.961380254672578E-4	1.379986560902306E-4	9.857046863587901E-6	2.6449742417294204E-4
84-85	0.8525573401665887	5.240663249140901E-4	1.379986560902306E-4	9.857046863587901E-6	2.727116298925986E-4
86-87	1.0658046920134494	5.634945123684416E-4	1.5442706752954378E-4	9.857046863587901E-6	2.8585435904404915E-4
88-89	1.3266878656697423	5.881371295274114E-4	1.5442706752954378E-4	9.857046863587901E-6	3.022827704833623E-4
90	1.5528233062907444	6.144225878303125E-4	1.642841143931317E-4	9.857046863587901E-6	3.0556845277122496E-4
>>END_MODULE
