##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654120_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	26838591
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.31672746903889	32.0	32.0	32.0	32.0	32.0
2	31.222386898030525	32.0	32.0	32.0	32.0	32.0
3	35.05431533272369	37.0	32.0	37.0	32.0	37.0
4	35.93024376726781	37.0	37.0	37.0	32.0	37.0
5	36.07261834274385	37.0	37.0	37.0	37.0	37.0
6	39.19527593680309	41.0	41.0	41.0	37.0	41.0
7	39.19717294398949	41.0	41.0	41.0	37.0	41.0
8	39.40872242510793	41.0	41.0	41.0	37.0	41.0
9	39.61512189667483	41.0	41.0	41.0	37.0	41.0
10-11	39.66852695433974	41.0	41.0	41.0	37.0	41.0
12-13	39.567185158863225	41.0	41.0	41.0	37.0	41.0
14-15	39.467740705911126	41.0	41.0	41.0	37.0	41.0
16-17	39.5502246187216	41.0	41.0	41.0	37.0	41.0
18-19	39.530858009647375	41.0	41.0	41.0	37.0	41.0
20-21	39.4870169786484	41.0	41.0	41.0	37.0	41.0
22-23	39.363074890928516	41.0	41.0	41.0	37.0	41.0
24-25	39.39829263019061	41.0	41.0	41.0	37.0	41.0
26-27	39.29983289733802	41.0	41.0	41.0	37.0	41.0
28-29	39.3866534759593	41.0	41.0	41.0	37.0	41.0
30-31	39.3569226491808	41.0	41.0	41.0	37.0	41.0
32-33	39.36180999591223	41.0	41.0	41.0	37.0	41.0
34-35	39.23008521572537	41.0	41.0	41.0	37.0	41.0
36-37	39.16065573263515	41.0	41.0	41.0	37.0	41.0
38-39	39.11232717097556	41.0	41.0	41.0	37.0	41.0
40-41	39.04229985471294	41.0	41.0	41.0	37.0	41.0
42-43	39.01892331083998	41.0	41.0	41.0	37.0	41.0
44-45	39.02159968084763	41.0	41.0	41.0	37.0	41.0
46-47	38.97205225863012	41.0	41.0	41.0	37.0	41.0
48-49	38.92719170689698	41.0	41.0	41.0	34.5	41.0
50-51	38.977365577798025	41.0	41.0	41.0	37.0	41.0
52-53	38.97478218957173	41.0	41.0	41.0	37.0	41.0
54-55	38.96210879699311	41.0	41.0	41.0	37.0	41.0
56-57	38.915890442981905	41.0	41.0	41.0	34.5	41.0
58-59	38.82151712062679	41.0	41.0	41.0	32.0	41.0
60-61	38.86466649832698	41.0	41.0	41.0	32.0	41.0
62-63	38.85458785820761	41.0	41.0	41.0	32.0	41.0
64-65	38.82587901130876	41.0	41.0	41.0	32.0	41.0
66-67	38.776931881409126	41.0	41.0	41.0	32.0	41.0
68-69	38.751600410021524	41.0	41.0	41.0	32.0	41.0
70-71	38.723104260577614	41.0	41.0	41.0	32.0	41.0
72-73	38.6916288004836	41.0	41.0	41.0	32.0	41.0
74-75	38.66905065917953	41.0	41.0	41.0	32.0	41.0
76-77	37.806162477009316	41.0	39.0	41.0	29.5	41.0
78-79	38.25855634522692	41.0	39.0	41.0	32.0	41.0
80-81	38.59606132080481	41.0	41.0	41.0	32.0	41.0
82-83	38.75959086302258	41.0	41.0	41.0	32.0	41.0
84-85	38.71586703266203	41.0	41.0	41.0	32.0	41.0
86-87	38.65069002690939	41.0	41.0	41.0	32.0	41.0
88-89	38.59409597918162	41.0	41.0	41.0	32.0	41.0
90-91	38.53991958817808	41.0	41.0	41.0	32.0	41.0
92-93	38.4710842868018	41.0	41.0	41.0	32.0	41.0
94-95	38.46205952093386	41.0	41.0	41.0	32.0	41.0
96-97	38.36536154226576	41.0	37.0	41.0	32.0	41.0
98-99	38.15799948290877	41.0	37.0	41.0	32.0	41.0
100-101	37.18183340921288	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	18.0
13	82.0
14	325.0
15	1387.0
16	6823.0
17	18359.0
18	32298.0
19	43358.0
20	52885.0
21	62145.0
22	73346.0
23	85987.0
24	100475.0
25	115716.0
26	133711.0
27	153690.0
28	177006.0
29	204068.0
30	238494.0
31	278916.0
32	326965.0
33	389373.0
34	472937.0
35	590683.0
36	766870.0
37	1073931.0
38	1760694.0
39	4348458.0
40	1.5329591E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.970499470409173	17.937963601650033	13.987530992199554	40.10400593574124
2	27.92500917801534	20.068285253871935	34.761154190247915	17.245551377864807
3	21.635469907530315	23.022394114430654	29.99750917757217	25.34462680046687
4	24.520564436486254	29.444626955267513	23.446514014092617	22.58829459415362
5	27.56312856922687	31.525374693363595	22.520299337346398	18.391197400063138
6	21.311863949936864	35.209873722506515	22.728234876413595	20.75002745114302
7	21.328403566342214	18.674381974821255	37.981099678444366	22.016114780392158
8	22.456100620185314	23.029990657855326	27.53737332932269	26.97653539263667
9	23.167475520603894	22.46086242008755	29.627945073569624	24.74371698573893
10-11	25.871757971646858	28.227798816861938	22.654247064804174	23.24619614668703
12-13	26.63189283101366	22.6211524288071	25.13765360015725	25.60930114002199
14-15	24.61830988072362	26.419779264865284	26.240626789983125	22.72128406442797
16-17	25.96130734109634	25.907455239096365	25.200672948207153	22.930564471600142
18-19	25.61890478627799	26.704550937663278	25.077821743096013	22.598722532962718
20-21	25.872207777342393	26.70092302474722	24.989286271659612	22.43758292625078
22-23	25.535141880187744	26.600982395060228	25.210131046865904	22.653744677886127
24-25	24.88782940497238	26.709688566365404	25.765520424742643	22.636961603919577
26-27	25.378534215898295	26.46847220854478	25.577477223003246	22.575516352553677
28-29	25.68856747277875	26.347302287894518	25.353317556223743	22.610812683102992
30-31	25.276932384416156	26.58365522988893	25.485743644291908	22.653668741403006
32-33	25.377430691773544	26.665300287068266	25.36029971221631	22.596969308941876
34-35	25.56319182329654	26.350576675206234	25.34558352932909	22.740647972168137
36-37	25.453197226337252	26.34395561227488	25.509915926659488	22.69293123472838
38-39	25.55843327593832	26.66938843065987	25.209454047354598	22.562724246047217
40-41	25.754114662725776	26.290087285133563	25.096021247911267	22.859776804229405
42-43	25.51677818514842	26.352350336839486	25.471225876870758	22.65964560114134
44-45	25.699971335792675	26.339163038680212	25.533975655506975	22.426889970020138
46-47	25.611486228999603	26.407695938716802	25.40878228386448	22.572035548419116
48-49	25.21163542202244	26.53102457898433	25.55332308701315	22.704016911980077
50-51	25.73384720531715	26.41984074350252	25.285658624925578	22.560653426254753
52-53	25.990823006312187	26.200426898439567	25.25997600768917	22.548774087559078
54-55	25.544001550759766	26.398422860181142	25.442585513716654	22.614990075342444
56-57	25.25327064240318	26.615358607188828	25.69475790282131	22.43661284758668
58-59	25.689053497629587	26.103771617518966	25.564037620305776	22.64313726454567
60-61	25.699358803728245	26.11076277347568	25.444399376658623	22.745479046137458
62-63	25.639227894431755	26.41832324933306	25.388340421799477	22.554108434435715
64-65	25.723271016723643	26.318972929689195	25.31903966195543	22.638716391631736
66-67	25.485043481568965	26.54352476334797	25.577431207985263	22.394000547097797
68-69	25.78101347216883	26.637837410859095	25.44587523640266	22.135273880569418
70-71	25.97565095655386	26.389676282203073	25.05457497323463	22.580097788008434
72-73	25.392268170858895	26.662176490561666	25.441272233702584	22.504283104876855
74-75	25.39884680352202	26.522887438904768	25.517688998744216	22.560576758828997
76-77	25.79617880081227	26.33358750960334	25.441541143961633	22.42869254562276
78-79	25.58206949090584	26.527124691456414	25.55638259847546	22.33442321916229
80-81	25.585561849165998	26.640731350286195	25.529196765135282	22.244510035412528
82-83	25.915451504353765	26.302681006937924	25.45792486189052	22.323942626817797
84-85	25.76344678588066	26.29992187346445	25.48032635597326	22.45630498468163
86-87	25.5442005133578	26.62870416707047	25.565384561357934	22.2617107582138
88-89	25.753512708855652	26.494473408612972	25.39907378002948	22.352940102501893
90-91	25.47745538409799	26.586755834818536	25.599166364272392	22.336622416811082
92-93	25.727899955632754	26.55289226844615	25.514734108109838	22.20447366781126
94-95	25.997907267188502	26.32126440616797	25.392601645891173	22.288226680752356
96-97	25.597991114190993	26.44089976803939	25.660004020797185	22.301105096972435
98-99	25.675828289755216	26.725699212532998	25.525550247898686	22.072922249813097
100-101	26.0397092497076	26.416142508577227	25.525331627516014	22.01881661419916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	12.0
1	13.0
2	10.5
3	12.0
4	23.0
5	31.0
6	35.0
7	49.5
8	61.0
9	71.0
10	96.5
11	123.0
12	170.5
13	239.0
14	305.5
15	399.0
16	494.5
17	632.0
18	842.0
19	1081.5
20	1418.0
21	1866.0
22	2456.0
23	3337.0
24	4499.5
25	5997.0
26	7920.5
27	10723.0
28	15094.5
29	19992.5
30	26400.5
31	36141.0
32	50194.5
33	68498.0
34	94172.0
35	137844.5
36	201885.5
37	279889.5
38	387832.0
39	537957.5
40	717540.0
41	916664.0
42	1131767.0
43	1329982.5
44	1498579.5
45	1619871.5
46	1681192.5
47	1714120.5
48	1684746.0
49	1582408.0
50	1447573.5
51	1323251.0
52	1218219.0
53	1098679.5
54	964499.5
55	838600.0
56	709909.5
57	588169.5
58	477361.5
59	392745.5
60	339316.5
61	285705.5
62	234323.0
63	190315.0
64	164413.0
65	147399.5
66	128141.0
67	109706.5
68	88188.0
69	66745.5
70	60357.0
71	54969.0
72	42159.5
73	32695.0
74	22666.0
75	13779.5
76	8996.0
77	4896.0
78	2792.5
79	1637.5
80	955.0
81	633.0
82	438.0
83	313.5
84	240.0
85	199.5
86	175.5
87	152.5
88	129.5
89	118.0
90	97.5
91	73.0
92	68.5
93	57.0
94	47.0
95	43.5
96	45.5
97	51.0
98	51.5
99	83.5
100	389.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04201412808891495
2	0.0
3	2.4591454894185764E-4
4	0.0
5	0.008372272598065972
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.8443591170639323E-4
12-13	4.37802416676792E-4
14-15	0.0
16-17	0.001006014063853054
18-19	4.4711736171246846E-5
20-21	0.0013469410521588113
22-23	3.7632377944132764E-4
24-25	0.002930481708223804
26-27	0.0
28-29	0.002060465841891625
30-31	0.0
32-33	0.0046444315947882665
34-35	0.0
36-37	0.0
38-39	0.0022504907206194243
40-41	0.0
42-43	7.992222840610374E-4
44-45	9.01686679453478E-4
46-47	0.0016226634252148333
48-49	0.0012593805688234528
50-51	0.0
52-53	0.003077657839787491
54-55	4.974180649051212E-4
56-57	0.0033422022788007016
58-59	0.0
60-61	2.6081846099893994E-5
62-63	0.003211793048301231
64-65	0.0
66-67	0.002274709577712183
68-69	4.2289850461970974E-4
70-71	0.002757223730560222
72-73	0.0
74-75	2.2169569184909892E-4
76-77	0.003414858850078978
78-79	0.0
80-81	0.003929043816048316
82-83	0.0013879268103157874
84-85	0.002677115203253405
86-87	0.0
88-89	9.613023276818071E-4
90-91	3.72597801427057E-5
92-93	3.670088344056512E-4
94-95	0.0
96-97	0.002345503159983324
98-99	2.2355868085623423E-5
100-101	8.737418443464487E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.6838591E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.910246227575918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.42758080088119	20.239897209755853
2	15.316170743706826	9.774855593905587
3	6.92375948001605	6.628166094834754
4	3.674436246862572	4.690086615396584
5	2.247136978249975	3.585334714152381
6	1.5326182837142033	2.934373608372302
7	1.1236528535968109	2.5099227462814784
8	0.8218401381713598	2.098009693500248
9	0.6364855868079485	1.8279370615810302
>10	3.819478699081127	22.4052959238128
>50	0.28737449378943236	6.342759859861468
>100	0.17147191991704624	10.408092186177683
>500	0.012178336583540976	2.6470623414703986
>1k	0.005496259290993447	3.025542857369952
>5k	2.3324643410988391E-4	0.5027831018154857
>10k+	8.593289677732565E-5	0.3798803917120245
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTTTCATTTGCATAGTAAGAAGAAGAAGATGTCTTGCTGCGGTGGAAAC	28906	0.1077031204805051	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.079358227114083E-5	3.7259780142705703E-6	1.4903912057082281E-5	0.0	0.0
2	7.45195602854114E-5	7.451956028541141E-6	1.862989007135285E-5	3.7259780142705703E-6	0.0
3	8.942347234249369E-5	7.451956028541141E-6	2.2355868085623423E-5	3.7259780142705703E-6	1.4903912057082281E-5
4	1.3413520851374052E-4	7.451956028541141E-6	5.5889670214058554E-5	7.451956028541141E-6	1.4903912057082281E-5
5	1.5649107659936395E-4	7.451956028541141E-6	8.197151631395255E-5	7.451956028541141E-6	1.4903912057082281E-5
6	1.6766901064217565E-4	7.451956028541141E-6	1.378611865280111E-4	7.451956028541141E-6	1.862989007135285E-5
7	1.9002487872779908E-4	7.451956028541141E-6	1.5649107659936395E-4	1.4903912057082281E-5	1.862989007135285E-5
8	2.0120281277061079E-4	7.451956028541141E-6	1.8257292269925794E-4	2.608184609989399E-5	1.862989007135285E-5
9	2.1610672482769307E-4	7.451956028541141E-6	1.8629890071352853E-4	2.9807824114164563E-5	1.862989007135285E-5
10-11	2.4964052695612823E-4	1.1177934042811711E-5	2.0492879078488137E-4	2.9807824114164563E-5	1.862989007135285E-5
12-13	3.185711202201338E-4	1.1177934042811711E-5	2.2728465887050477E-4	2.9807824114164563E-5	2.0492879078488137E-5
14-15	3.9309068050554516E-4	1.1177934042811711E-5	2.5150351596326347E-4	2.9807824114164563E-5	2.2355868085623423E-5
16-17	5.141849659693387E-4	1.1177934042811711E-5	4.1544654859116857E-4	2.9807824114164563E-5	2.2355868085623423E-5
18-19	6.874429436329202E-4	1.1177934042811711E-5	4.433913836981979E-4	3.353380212843513E-5	2.2355868085623423E-5
20-21	9.240425475391014E-4	1.4903912057082281E-5	5.085959989479329E-4	3.72597801427057E-5	2.421885709275871E-5
22-23	0.0013897897993229227	1.4903912057082281E-5	6.091974053332382E-4	3.72597801427057E-5	4.284874716411156E-5
24-25	0.002006439160684702	1.6766901064217565E-5	6.390052294474028E-4	3.72597801427057E-5	4.4711736171246846E-5
26-27	0.002785168565667251	1.862989007135285E-5	6.781279985972439E-4	4.0985758156976274E-5	4.843771418551742E-5
28-29	0.0036626363880279704	2.0492879078488137E-5	7.377436468255729E-4	4.4711736171246846E-5	5.216369219978798E-5
30-31	0.004694732297980919	2.2355868085623423E-5	7.489215808683847E-4	4.657472517838213E-5	5.216369219978798E-5
32-33	0.005864689394461877	2.421885709275871E-5	7.656884819326022E-4	4.843771418551742E-5	5.216369219978798E-5
34-35	0.00708867317214976	2.7944835107029277E-5	9.836581957674306E-4	4.843771418551742E-5	5.216369219978798E-5
36-37	0.008413258356222947	2.9807824114164563E-5	9.966991188173776E-4	4.843771418551742E-5	5.216369219978798E-5
38-39	0.009961402221152368	2.9807824114164563E-5	0.0010209179759101362	5.0300703192652696E-5	5.216369219978798E-5
40-41	0.011954800458787124	3.353380212843513E-5	0.0010898485691741419	5.216369219978798E-5	5.216369219978798E-5
42-43	0.014263043838627743	4.843771418551742E-5	0.0011364232943525238	5.5889670214058554E-5	5.216369219978798E-5
44-45	0.016802297855353136	5.402668120692327E-5	0.0011513272064096062	5.5889670214058554E-5	5.5889670214058554E-5
46-47	0.019425386377399618	5.775265922119384E-5	0.0012482026347806412	5.5889670214058554E-5	5.5889670214058554E-5
48-49	0.02242293568988029	5.9615648228329125E-5	0.0013376261071231348	5.5889670214058554E-5	5.5889670214058554E-5
50-51	0.026242063154507625	5.9615648228329125E-5	0.001352530019180217	5.5889670214058554E-5	5.5889670214058554E-5
52-53	0.030715099760639446	6.147863723546441E-5	0.0014009677333657344	5.5889670214058554E-5	6.147863723546441E-5
54-55	0.035789881816075966	6.334162624259969E-5	0.0015313769638652043	5.5889670214058554E-5	6.334162624259969E-5
56-57	0.040941046420805025	6.334162624259969E-5	0.0017120868975573269	5.5889670214058554E-5	6.334162624259969E-5
58-59	0.04644245295887553	6.334162624259969E-5	0.001782880479828468	5.5889670214058554E-5	6.334162624259969E-5
60-61	0.05315107637356968	6.334162624259969E-5	0.0018853448752209085	5.5889670214058554E-5	6.334162624259969E-5
62-63	0.06141715859822894	6.520461524973497E-5	0.0020157541057203784	5.5889670214058554E-5	6.334162624259969E-5
64-65	0.07118853594065352	6.706760425687026E-5	0.0020809587209701134	5.775265922119384E-5	7.079358227114083E-5
66-67	0.08213173336856619	7.265657127827611E-5	0.0020921366550129255	5.9615648228329125E-5	7.45195602854114E-5
68-69	0.09450943233197301	9.314945035676426E-5	0.0021070405670700074	5.9615648228329125E-5	7.45195602854114E-5
70-71	0.10927920918054156	9.314945035676426E-5	0.0021182185011128195	5.9615648228329125E-5	8.197151631395255E-5
72-73	0.12732412070365393	9.687542837103483E-5	0.0021796971383482838	5.9615648228329125E-5	8.569749432822312E-5
74-75	0.1495887023279277	9.687542837103483E-5	0.0021796971383482838	5.9615648228329125E-5	8.569749432822312E-5
76-77	0.17641201805266155	1.0060140638530539E-4	0.002190875072391095	5.9615648228329125E-5	8.569749432822312E-5
78-79	0.20975952128038317	1.0246439539244069E-4	0.0022001900174267717	6.147863723546441E-5	8.569749432822312E-5
80-81	0.2498044699887561	1.0619037340671124E-4	0.0022057789844481777	6.520461524973497E-5	9.314945035676426E-5
82-83	0.29749885156042655	1.0805336241384654E-4	0.0022560796876408304	6.706760425687026E-5	9.314945035676426E-5
84-85	0.3549180357493432	1.1177934042811711E-4	0.0022709835996979127	6.706760425687026E-5	9.314945035676426E-5
86-87	0.4279192599939393	1.1177934042811711E-4	0.0022896134897692653	7.079358227114083E-5	9.314945035676426E-5
88-89	0.5183953211254644	1.1923129645665825E-4	0.002298928434804942	7.265657127827611E-5	9.314945035676426E-5
90	0.5972481938414725	1.2295727447092882E-4	0.002306380390833483	7.45195602854114E-5	9.314945035676426E-5
>>END_MODULE
