##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654116_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30594634
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25693829185863	32.0	32.0	32.0	32.0	32.0
2	31.372136957088617	32.0	32.0	32.0	32.0	32.0
3	34.79251420363453	37.0	32.0	37.0	32.0	37.0
4	35.70810691835699	37.0	37.0	37.0	32.0	37.0
5	35.8663816341127	37.0	37.0	37.0	37.0	37.0
6	39.19230650054516	41.0	41.0	41.0	37.0	41.0
7	39.42064206422604	41.0	41.0	41.0	37.0	41.0
8	39.53960668396948	41.0	41.0	41.0	37.0	41.0
9	39.4643327323347	41.0	41.0	41.0	37.0	41.0
10-11	39.46933293923372	41.0	41.0	41.0	37.0	41.0
12-13	39.53456295636679	41.0	41.0	41.0	37.0	41.0
14-15	39.46193915900416	41.0	41.0	41.0	37.0	41.0
16-17	39.39733609821906	41.0	41.0	41.0	37.0	41.0
18-19	39.42689690290134	41.0	41.0	41.0	37.0	41.0
20-21	39.44659543892567	41.0	41.0	41.0	37.0	41.0
22-23	39.24662006089042	41.0	41.0	41.0	37.0	41.0
24-25	39.26626113585801	41.0	41.0	41.0	37.0	41.0
26-27	39.278946579324995	41.0	41.0	41.0	37.0	41.0
28-29	39.193419636920645	41.0	41.0	41.0	37.0	41.0
30-31	39.21878692845288	41.0	41.0	41.0	37.0	41.0
32-33	39.327178468616424	41.0	41.0	41.0	37.0	41.0
34-35	39.281802537660695	41.0	41.0	41.0	37.0	41.0
36-37	39.12657244077507	41.0	41.0	41.0	37.0	41.0
38-39	39.04782827276182	41.0	41.0	41.0	37.0	41.0
40-41	38.96611561033873	41.0	41.0	41.0	37.0	41.0
42-43	38.9446900721218	41.0	41.0	41.0	37.0	41.0
44-45	38.94664137835413	41.0	41.0	41.0	37.0	41.0
46-47	38.89155026335664	41.0	41.0	41.0	34.5	41.0
48-49	38.840616217209856	41.0	41.0	41.0	32.0	41.0
50-51	38.887099139672664	41.0	41.0	41.0	32.0	41.0
52-53	38.88298095018885	41.0	41.0	41.0	32.0	41.0
54-55	38.87310126017523	41.0	41.0	41.0	32.0	41.0
56-57	38.83271870485524	41.0	41.0	41.0	32.0	41.0
58-59	38.734419179520174	41.0	41.0	41.0	32.0	41.0
60-61	38.77268763862317	41.0	41.0	41.0	32.0	41.0
62-63	38.764859599235606	41.0	41.0	41.0	32.0	41.0
64-65	38.73995753634445	41.0	41.0	41.0	32.0	41.0
66-67	38.70158548064344	41.0	41.0	41.0	32.0	41.0
68-69	38.68132251557577	41.0	41.0	41.0	32.0	41.0
70-71	38.65207549140807	41.0	41.0	41.0	32.0	41.0
72-73	38.6227870384068	41.0	41.0	41.0	32.0	41.0
74-75	38.600545425710926	41.0	41.0	41.0	32.0	41.0
76-77	37.733620722509706	41.0	39.0	41.0	29.5	41.0
78-79	38.19068927250446	41.0	39.0	41.0	32.0	41.0
80-81	38.540594161054514	41.0	41.0	41.0	32.0	41.0
82-83	38.70812072796818	41.0	41.0	41.0	32.0	41.0
84-85	38.661841190844115	41.0	41.0	41.0	32.0	41.0
86-87	38.598399575559554	41.0	41.0	41.0	32.0	41.0
88-89	38.53709049109723	41.0	41.0	41.0	32.0	41.0
90-91	38.480856544974515	41.0	41.0	41.0	32.0	41.0
92-93	38.41135782176704	41.0	39.0	41.0	32.0	41.0
94-95	38.394540689717026	41.0	37.0	41.0	32.0	41.0
96-97	38.293918453150965	41.0	37.0	41.0	32.0	41.0
98-99	38.08551862395216	41.0	37.0	41.0	32.0	41.0
100-101	37.1000951016443	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	29.0
13	109.0
14	370.0
15	1863.0
16	8568.0
17	23951.0
18	41511.0
19	55511.0
20	66788.0
21	78005.0
22	90740.0
23	105530.0
24	122465.0
25	139939.0
26	159293.0
27	182786.0
28	210770.0
29	241461.0
30	279245.0
31	324525.0
32	380985.0
33	451571.0
34	546018.0
35	678468.0
36	877634.0
37	1230463.0
38	2041163.0
39	5130187.0
40	1.7124686E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.03993142805048	21.55504873665705	11.432936424747679	33.97208341054479
2	29.296114475499202	25.580263519413244	29.035853149934727	16.087768855152834
3	21.102568741398592	28.209875907657423	28.606446473890536	22.08110887705345
4	24.727542091204622	31.222216941702914	23.460973581184202	20.58926738590826
5	27.66798863429276	32.594645173047546	21.72661339025449	18.010752802405204
6	22.67481611317854	37.205089624540044	21.68259309786154	18.437501164419878
7	22.319613302123503	22.41956547020631	35.391562455037054	19.869258772633135
8	23.23398279580661	25.00333555224096	26.977704652391004	24.784976999561426
9	23.48864183176697	23.59563118159871	29.584338220878863	23.33138876575546
10-11	25.099636935668794	30.309808489595767	23.382321207372105	21.208233367363327
12-13	25.591357048682895	25.385957405485556	25.982427300747457	23.040258245084093
14-15	24.734476313722205	27.018992938434888	26.04561146245449	22.200919285388412
16-17	25.614321128655238	26.537325775873988	25.534561226813324	22.31379186865745
18-19	25.158017156022293	27.03080224461679	25.657534701933386	22.153645897427523
20-21	25.3992378522602	27.042364680925875	25.498310553933596	22.060086912880333
22-23	25.415264604988693	26.85708448069261	25.534431335746337	22.193219578572364
24-25	24.845577013511495	26.94740807830258	25.965149589969894	22.241865318216032
26-27	25.107661036245766	27.057243110017264	25.810710793271785	22.02438506046518
28-29	25.328413148322376	26.67265346704007	25.71528511752716	22.2836482671104
30-31	24.905321632545103	26.887603885047294	25.95982844573333	22.247246036674273
32-33	25.070502185608206	27.055881257013304	25.729674801431774	22.143941755946717
34-35	25.304380173333662	26.798145387194367	25.650669656646325	22.24680478282564
36-37	24.924014452991987	26.804883496890337	26.0413966710633	22.22970537905438
38-39	25.10811576507343	27.05820604846858	25.714363227295728	22.11931495916226
40-41	25.3849972514788	26.708172093184707	25.63616547921443	22.270665176122062
42-43	24.891285617479912	26.819148989023738	26.059365854940452	22.230199538555894
44-45	25.085628078456057	26.92321263178628	25.83926172440623	22.151897565351433
46-47	25.317536010218433	26.73600916594176	25.70478842043172	22.241666403408093
48-49	24.89169442925912	26.786476859603404	26.044869579144432	22.27695913199305
50-51	25.17475450106708	26.872503197782983	25.701188319494193	22.251553981655736
52-53	25.397653766170603	26.701838650046167	25.663860808323786	22.23664677545944
54-55	24.94075773713783	26.787244760304397	26.049827940487937	22.222169562069837
56-57	25.003235971345116	26.975960916787944	25.852425128448452	22.16837798341849
58-59	25.31197464235068	26.577358304073844	25.7771967463314	22.333470307244074
60-61	25.001013658428338	26.717896906435413	25.99970213722668	22.281387297909564
62-63	25.113290311640146	26.845598327142255	25.86733930330052	22.17377205791708
64-65	25.30847239421135	26.649511479692812	25.834461690242804	22.207554435853034
66-67	24.955424551656357	26.796481803710282	26.091439652436772	22.156653992196585
68-69	25.18250206103103	26.989271852383357	25.786675668279457	22.041550418306155
70-71	25.509311692932997	26.71088166639703	25.605298569736373	22.1745080709336
72-73	24.9761052869598	26.8541862602442	26.063107994689528	22.106600458106477
74-75	25.03499559480581	26.96340346724982	25.865234560452162	22.136366377492212
76-77	25.395949188280465	26.688192866135406	25.725616479174125	22.19024146641
78-79	24.95932456652366	26.858131396505673	26.08718738063675	22.095356656333916
80-81	25.203594393122238	26.937502847834043	25.820164763501808	22.03873799554192
82-83	25.44207584688896	26.677720163085596	25.75586856362935	22.1243354263961
84-85	25.128298896174826	26.85133788898494	26.031717413015205	21.988645801825037
86-87	25.254761995191704	27.027953660109155	25.792521329067053	21.924763015632088
88-89	25.4209434584805	26.82107986606216	25.680440799173297	22.077535876284045
90-91	25.047958178144718	26.915362961380257	26.003420110502738	22.03325874997228
92-93	25.3264570710161	27.023515446945286	25.737032125483356	21.912995356555264
94-95	25.665570962541995	26.74657588647735	25.644786271997894	21.943066878982766
96-97	25.21097173986403	26.847581878065398	25.983573265631836	21.957873116438737
98-99	25.42833785348954	27.108701450648436	25.658558004716415	21.804402691145604
100-101	25.749654364466974	26.79842752747352	25.613805822941387	21.838112285118115
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	20.0
2	24.0
3	35.0
4	44.5
5	58.0
6	70.5
7	86.5
8	114.5
9	151.5
10	191.5
11	235.0
12	289.0
13	350.0
14	416.5
15	510.5
16	644.0
17	813.5
18	1022.5
19	1325.5
20	1697.0
21	2216.5
22	2867.0
23	3798.0
24	5034.5
25	6637.5
26	8815.5
27	11800.5
28	16534.5
29	22268.0
30	29662.5
31	40511.0
32	56587.0
33	80301.0
34	117122.0
35	175667.5
36	261748.0
37	380939.0
38	546273.0
39	757543.0
40	996018.0
41	1247764.0
42	1503668.5
43	1722864.5
44	1888023.0
45	1991331.0
46	2026299.0
47	2008919.5
48	1930400.0
49	1791375.5
50	1612298.0
51	1442532.0
52	1284243.0
53	1122854.5
54	969601.0
55	822172.0
56	671780.5
57	534691.5
58	430185.0
59	356226.5
60	303046.5
61	254671.0
62	209954.5
63	173332.0
64	150595.0
65	129936.0
66	109014.0
67	92270.5
68	73651.5
69	56037.0
70	45334.0
71	36063.5
72	25726.0
73	17642.0
74	10928.5
75	6520.0
76	3959.5
77	2176.5
78	1262.0
79	845.5
80	572.0
81	415.0
82	319.0
83	271.5
84	240.5
85	195.0
86	165.0
87	148.5
88	121.5
89	100.5
90	86.0
91	74.0
92	67.5
93	59.0
94	46.0
95	37.5
96	34.0
97	43.0
98	54.0
99	91.5
100	452.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0425989734016756
2	0.0
3	2.582152151256328E-4
4	0.0
5	0.008527639193199696
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.830386335067777E-4
12-13	4.2817966052478357E-4
14-15	0.0
16-17	0.0010246894929352645
18-19	4.249111134978768E-5
20-21	0.0014201836831909803
22-23	3.4319743782520816E-4
24-25	0.0029580350593506038
26-27	0.0
28-29	0.002165412405325718
30-31	0.0
32-33	0.004685462163070818
34-35	0.0
36-37	0.0
38-39	0.002260200269106014
40-41	0.0
42-43	8.040625686190592E-4
44-45	8.481879534823002E-4
46-47	0.0016522505221013594
48-49	0.0013368357340048584
50-51	0.0
52-53	0.003155782154478462
54-55	4.330824810651436E-4
56-57	0.003392751813929201
58-59	0.0
60-61	2.7782649728707328E-5
62-63	0.0032636442063663845
64-65	0.0
66-67	0.002348451038832496
68-69	3.807857286346358E-4
70-71	0.0028387330928685076
72-73	0.0
74-75	2.2552974485656534E-4
76-77	0.003471196942574963
78-79	0.0
80-81	0.003948404808503348
82-83	0.0012845389815743504
84-85	0.0027455795026016653
86-87	0.0
88-89	9.593185523971296E-4
90-91	2.7782649728707328E-5
92-93	3.464659848521149E-4
94-95	0.0
96-97	0.0023729651415342963
98-99	1.7977008647987095E-5
100-101	8.759706032110075E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.0594634E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.162559452155016
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.390364916558994	21.65577110155748
2	14.99784088755507	10.247292619501227
3	6.747686693764641	6.915547435207498
4	3.8624462486486792	5.278041984008546
5	2.406345988827842	4.110346895289295
6	1.657809363527013	3.3981006565098513
7	1.1512510189114133	2.753077696454064
8	0.8746014198413121	2.3902898401814414
9	0.6833341094391714	2.1009997925460984
>10	3.829065963263736	23.715993299361546
>50	0.2580531683954628	6.0548506674606575
>100	0.13083011747990686	8.129052723435946
>500	0.007743617818589836	1.8056404225971454
>1k	0.002606511421423364	1.3850544631282662
>5k	9.987273279407881E-6	0.02315320885333881
>10k+	9.987273279407881E-6	0.03678719390755954
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6148376215253955E-5	9.805641080720233E-6	6.537094053813489E-6	0.0	0.0
2	3.595401729597419E-5	4.249111134978768E-5	1.6342735134533722E-5	3.2685470269067444E-6	0.0
3	8.498222269957535E-5	5.229675243050791E-5	1.9611282161440465E-5	3.2685470269067444E-6	9.805641080720233E-6
4	1.3727897513008327E-4	5.5565299457414656E-5	3.2685470269067444E-5	3.2685470269067444E-6	9.805641080720233E-6
5	1.5689025729152372E-4	6.210239351122814E-5	3.595401729597419E-5	3.2685470269067444E-6	9.805641080720233E-6
6	2.0264991566821815E-4	6.210239351122814E-5	3.922256432288093E-5	3.2685470269067444E-6	9.805641080720233E-6
7	2.2552974485656537E-4	6.863948756504164E-5	3.922256432288093E-5	3.2685470269067444E-6	9.805641080720233E-6
8	2.582152151256328E-4	6.863948756504164E-5	5.229675243050791E-5	3.2685470269067444E-6	9.805641080720233E-6
9	2.778264972870733E-4	7.190803459194838E-5	6.210239351122814E-5	3.2685470269067444E-6	1.3074188107626977E-5
10-11	3.464659848521149E-4	8.498222269957535E-5	7.190803459194837E-5	3.2685470269067444E-6	2.124555567489384E-5
12-13	3.9712846376916945E-4	9.315359026684222E-5	8.334794918612198E-5	3.2685470269067444E-6	2.2879829188347212E-5
14-15	4.723050453880246E-4	9.805641080720234E-5	1.0295923134756246E-4	6.537094053813489E-6	2.2879829188347212E-5
16-17	5.687271826817736E-4	1.0949632540137594E-4	1.7486726593951083E-4	1.1439914594173606E-5	3.2685470269067444E-5
18-19	6.912976961907765E-4	1.1930196648209618E-4	2.0918700972203164E-4	1.6342735134533722E-5	3.595401729597419E-5
20-21	8.547250475361137E-4	1.2093623999554955E-4	2.451410270180058E-4	1.6342735134533722E-5	3.595401729597419E-5
22-23	0.0010639120572581455	1.2420478702245628E-4	2.94169232421607E-4	1.6342735134533722E-5	3.595401729597419E-5
24-25	0.0014299893242717006	1.3727897513008327E-4	3.121462410695941E-4	1.6342735134533722E-5	3.595401729597419E-5
26-27	0.0019022943696597253	1.5362171026461698E-4	3.301232497175812E-4	1.6342735134533722E-5	3.595401729597419E-5
28-29	0.0024416046290993377	1.6342735134533722E-4	3.7098008755391554E-4	1.9611282161440465E-5	3.922256432288093E-5
30-31	0.003181930530693716	1.9774709512785803E-4	3.873228226884492E-4	1.9611282161440465E-5	3.922256432288093E-5
32-33	0.00406116968093163	2.1572410377584513E-4	3.9876273728262285E-4	1.9611282161440465E-5	3.922256432288093E-5
34-35	0.005041733789003654	2.1899265080275188E-4	4.5759658376694424E-4	1.9611282161440465E-5	4.5759658376694424E-5
36-37	0.006095840205181079	2.2062692431620525E-4	5.082590626839988E-4	1.9611282161440465E-5	5.5565299457414656E-5
38-39	0.007499681153237525	2.3206683891037886E-4	5.49115900520333E-4	1.9611282161440465E-5	5.5565299457414656E-5
40-41	0.009285942103442062	2.5331239458527266E-4	5.88338464843214E-4	1.9611282161440465E-5	5.88338464843214E-5
42-43	0.011484039979036846	2.5658094161217946E-4	6.422694907871752E-4	1.9611282161440465E-5	5.88338464843214E-5
44-45	0.013977941360566692	2.6638658269289965E-4	6.635150464620692E-4	2.2879829188347212E-5	5.88338464843214E-5
46-47	0.016612390264253526	2.6802085620635305E-4	7.239831664598438E-4	2.2879829188347212E-5	6.863948756504164E-5
48-49	0.019972456607913663	2.7619222377361994E-4	7.484972691616445E-4	2.4514102701800585E-5	6.863948756504164E-5
50-51	0.024452000308289354	3.0070632647542046E-4	7.599371837558181E-4	2.94169232421607E-5	6.863948756504164E-5
52-53	0.02988105691998146	3.186833351234076E-4	8.073311156459659E-4	2.94169232421607E-5	6.863948756504164E-5
54-55	0.03605043943326794	3.252204291772211E-4	8.498222269957537E-4	2.94169232421607E-5	7.844512864576186E-5
56-57	0.04271010400059043	3.301232497175812E-4	8.923133383455413E-4	2.94169232421607E-5	8.171367567266861E-5
58-59	0.050765438148402106	3.333917967444879E-4	9.34804449695329E-4	2.94169232421607E-5	8.171367567266861E-5
60-61	0.06065606145182191	3.3829461728484807E-4	0.0010197866723949043	2.94169232421607E-5	8.171367567266861E-5
62-63	0.07308471152163482	3.4973453187902166E-4	0.0011211116302290134	2.94169232421607E-5	8.334794918612198E-5
64-65	0.08816578750378253	3.5790589944628855E-4	0.0011913853913075082	2.94169232421607E-5	8.661649621302873E-5
66-67	0.10567212537989504	3.807857286346357E-4	0.0012044595794151354	2.94169232421607E-5	8.82507697264821E-5
68-69	0.1261740866061676	3.938599167422627E-4	0.0012224365880631224	3.2685470269067444E-5	8.82507697264821E-5
70-71	0.15138765837172624	4.003970107960762E-4	0.0012289736821169358	3.2685470269067444E-5	9.151931675338885E-5
72-73	0.18281964085597496	4.0856837836334304E-4	0.00126002487887255	3.2685470269067444E-5	9.151931675338885E-5
74-75	0.22163690534751945	4.167397459306099E-4	0.00126492769941291	3.595401729597419E-5	9.151931675338885E-5
76-77	0.2688265530484856	4.2164256647097E-4	0.0012747333404936304	3.595401729597419E-5	9.151931675338885E-5
78-79	0.3262026275588066	4.2327683998442337E-4	0.0012878075286012571	3.595401729597419E-5	9.47878637802956E-5
80-81	0.3942014799065745	4.34716754578597E-4	0.001317224451843418	3.758829080942756E-5	9.805641080720234E-5
82-83	0.4771866203727098	4.3798530160550376E-4	0.0013335671869779517	3.922256432288093E-5	9.805641080720234E-5
84-85	0.5777908635873859	4.412538486324105E-4	0.001356447016166299	4.249111134978768E-5	9.805641080720234E-5
86-87	0.7018175147968758	4.4452239565931725E-4	0.0013662526572470193	4.412538486324105E-5	9.969068432065571E-5
88-89	0.8506475351200475	4.60865130793851E-4	0.0013809611188680996	4.5759658376694424E-5	1.0132495783410907E-4
90	0.9792403465261261	4.772078659283847E-4	0.001382595392381553	4.5759658376694424E-5	1.0459350486101582E-4
>>END_MODULE
