##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654140_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34752666
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.654674982345238	32.0	32.0	32.0	32.0	32.0
2	31.69135777957294	32.0	32.0	32.0	32.0	32.0
3	35.80881901261906	37.0	37.0	37.0	32.0	37.0
4	36.372594177379085	37.0	37.0	37.0	37.0	37.0
5	36.552907653185514	37.0	37.0	37.0	37.0	37.0
6	40.157479831907	41.0	41.0	41.0	37.0	41.0
7	40.18650733155263	41.0	41.0	41.0	37.0	41.0
8	40.224297381962	41.0	41.0	41.0	41.0	41.0
9	40.27723709024223	41.0	41.0	41.0	41.0	41.0
10-11	40.31066884192424	41.0	41.0	41.0	41.0	41.0
12-13	40.295207697159114	41.0	41.0	41.0	41.0	41.0
14-15	40.28922073201521	41.0	41.0	41.0	41.0	41.0
16-17	40.284753117933455	41.0	41.0	41.0	41.0	41.0
18-19	40.28121550444504	41.0	41.0	41.0	41.0	41.0
20-21	40.25028361852872	41.0	41.0	41.0	41.0	41.0
22-23	40.25590979120854	41.0	41.0	41.0	41.0	41.0
24-25	40.24278299972727	41.0	41.0	41.0	41.0	41.0
26-27	40.16224241616456	41.0	41.0	41.0	41.0	41.0
28-29	40.17066136738977	41.0	41.0	41.0	41.0	41.0
30-31	40.08333395199091	41.0	41.0	41.0	39.0	41.0
32-33	40.10644144538436	41.0	41.0	41.0	37.0	41.0
34-35	40.11573392671515	41.0	41.0	41.0	41.0	41.0
36-37	40.10257362701324	41.0	41.0	41.0	37.0	41.0
38-39	40.02524830756869	41.0	41.0	41.0	37.0	41.0
40-41	40.05348709074579	41.0	41.0	41.0	37.0	41.0
42-43	40.0352177441581	41.0	41.0	41.0	37.0	41.0
44-45	40.033793738299096	41.0	41.0	41.0	37.0	41.0
46-47	40.021842252332526	41.0	41.0	41.0	37.0	41.0
48-49	39.93250599824485	41.0	41.0	41.0	37.0	41.0
50-51	39.88799410381925	41.0	41.0	41.0	37.0	41.0
52-53	39.93372665279838	41.0	41.0	41.0	37.0	41.0
54-55	39.90346726205121	41.0	41.0	41.0	37.0	41.0
56-57	39.88961079993115	41.0	41.0	41.0	37.0	41.0
58-59	39.89080455007394	41.0	41.0	41.0	37.0	41.0
60-61	39.86938661051212	41.0	41.0	41.0	37.0	41.0
62-63	39.82848738856467	41.0	41.0	41.0	37.0	41.0
64-65	39.775173090317736	41.0	41.0	41.0	37.0	41.0
66-67	39.75018984155057	41.0	41.0	41.0	37.0	41.0
68-69	39.72745606049332	41.0	41.0	41.0	37.0	41.0
70-71	39.70104070576916	41.0	41.0	41.0	37.0	41.0
72-73	39.67144286139084	41.0	41.0	41.0	37.0	41.0
74-75	39.65347662823911	41.0	41.0	41.0	37.0	41.0
76-77	39.11591425820396	41.0	39.0	41.0	34.5	41.0
78-79	39.45084359858895	41.0	41.0	41.0	37.0	41.0
80-81	39.638548219581196	41.0	41.0	41.0	37.0	41.0
82-83	39.711010386944125	41.0	41.0	41.0	37.0	41.0
84-85	39.748249371717264	41.0	41.0	41.0	37.0	41.0
86-87	39.75200481022089	41.0	41.0	41.0	37.0	41.0
88-89	39.6349485245247	41.0	41.0	41.0	37.0	41.0
90-91	39.654100076811375	41.0	41.0	41.0	37.0	41.0
92-93	39.60570741536779	41.0	41.0	41.0	37.0	41.0
94-95	39.597978252949	41.0	41.0	41.0	37.0	41.0
96-97	39.56295741454771	41.0	41.0	41.0	37.0	41.0
98-99	39.5158241241118	41.0	41.0	41.0	37.0	41.0
100-101	38.98965269312001	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	3.0
16	12.0
17	34.0
18	62.0
19	209.0
20	636.0
21	1819.0
22	4534.0
23	9329.0
24	17802.0
25	30503.0
26	49953.0
27	76609.0
28	109996.0
29	150552.0
30	196516.0
31	247913.0
32	304825.0
33	368160.0
34	443851.0
35	542760.0
36	686242.0
37	929234.0
38	1445679.0
39	3421121.0
40	2.571431E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.6776435946902	12.057164500173396	5.98262515441276	44.28256675072364
2	24.215670245269816	13.525785331116754	32.06722327432376	30.191321149289667
3	20.647970916741865	15.554222643229407	21.74174393657575	42.05606250345297
4	23.788606606468697	22.94749128023732	21.16839899419515	32.09550311909884
5	24.775155379446286	27.559065540468175	23.749910294651926	23.915868785433613
6	20.75846497647116	30.97690404528965	25.033662165659464	23.23096881257973
7	16.233911378194698	25.21756460353286	39.51910049145582	19.02942352681662
8	18.780323213188883	24.404674450011978	31.560660698664094	25.254341638135042
9	18.851532138570317	22.690365683024147	33.95078812083079	24.507314057574746
10-11	21.51174533206789	30.841506393484874	24.841856641799478	22.80489163264776
12-13	21.940767076689884	24.93260517049253	27.332888648024873	25.793739104792706
14-15	21.437766817659398	25.739379246472776	27.307786976688348	25.515066959179478
16-17	21.948880301194336	26.152146050231295	26.640065074477842	25.258908574096523
18-19	21.735926237509627	26.3989752869276	26.538473845455446	25.326624630107318
20-21	21.947110474920112	26.323049575534725	26.686155531204424	25.04368441834074
22-23	21.97167405804205	26.531563074901936	26.585110045945825	24.911652821110184
24-25	21.906185325924895	26.2023128441269	26.426595195418773	25.464906634529427
26-27	21.668124684304797	26.322291360323263	26.696100092004453	25.31348386336749
28-29	21.804271949300087	26.463169654868075	26.57772666340667	25.15483173242517
30-31	21.867171108335352	26.234996745734872	26.598832464313542	25.29899968161623
32-33	21.9342869982982	26.150091621747812	26.680135849146076	25.23548553080791
34-35	22.07508051324753	26.239438723924085	26.363240736696287	25.322240026132096
36-37	21.89115968672964	26.1050853782712	26.364359015857357	25.6393959191418
38-39	21.938763045670648	26.21853664959861	26.632141316517714	25.210558988213023
40-41	22.128854804981003	26.379364679532785	26.48658091439661	25.005199601089593
42-43	22.123427883201824	26.036160794110014	26.384903822918222	25.455507499769947
44-45	22.097752155187173	26.055267241943397	26.549017850889484	25.297962751979945
46-47	22.13847492632885	26.181007678097846	26.39641459619123	25.28410279938207
48-49	22.06637326759334	26.039364864842312	26.415383498923507	25.47887836864084
50-51	22.06774210507188	26.016933396903497	26.58188400586289	25.333440492161735
52-53	22.463418813526914	26.09599684857159	26.29074401708424	25.14984032081725
54-55	22.351867911371173	25.939799841543092	26.246199356331395	25.462132890754337
56-57	22.200825642758115	25.856541668103617	26.602503688985635	25.34012900015264
58-59	22.219768850253406	26.00625857794964	26.496073404301022	25.27789916749593
60-61	22.074908244210462	25.85003934847138	26.480122770568613	25.59492963674954
62-63	22.24006044972649	25.816369999564337	26.663155933141574	25.2804136175676
64-65	22.46482782025962	25.949039031428693	26.372330154875538	25.21380299343614
66-67	22.22917443353154	25.762403512949867	26.428360260830843	25.580061792687754
68-69	22.219875950637444	25.791632504292167	26.615432804451554	25.37305874061883
70-71	22.413173745911898	26.019479596250765	26.419661017007677	25.14768564082966
72-73	22.351207530380545	25.865333612103313	26.35327315607959	25.430185701436546
74-75	22.320120706710675	25.8184321743834	26.593073463773976	25.268373655131953
76-77	22.349236863541535	25.960545127663575	26.423017629332634	25.267200379462256
78-79	22.234726459107524	25.765813931104276	26.45902983267614	25.540429777112056
80-81	22.28477423853064	25.823114633077093	26.600998128024372	25.291113000367893
82-83	22.430180376161967	26.0624286233553	26.278110974295082	25.229280026187645
84-85	22.473680359249634	25.787931795377155	26.289194159215267	25.449193686157944
86-87	22.438581633196854	25.797031955402787	26.508433626911916	25.255952784488443
88-89	22.60618940716663	25.969371673528585	26.38806760897135	25.03637131033343
90-91	22.502801138745127	25.80027910961397	26.343891957910376	25.353027793730526
92-93	22.47603811066374	25.775355150921374	26.437852919412343	25.310753819002546
94-95	22.71273087365441	25.887760668490873	26.20912450285109	25.190383955003625
96-97	22.631339669353675	25.89015319341189	26.038083253781668	25.440423883452766
98-99	22.67030940285475	25.975450808745848	26.255528956170192	25.09871083222921
100-101	22.70878585257315	26.132219611583178	26.10695824026853	25.052036295575135
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	44.0
1	36.0
2	25.0
3	25.5
4	37.0
5	48.0
6	66.5
7	80.0
8	101.5
9	132.0
10	151.5
11	182.0
12	227.0
13	266.0
14	300.5
15	366.0
16	450.0
17	558.0
18	713.5
19	914.0
20	1249.0
21	1744.5
22	2424.0
23	3505.5
24	5351.0
25	8084.0
26	11634.5
27	16962.5
28	25041.5
29	36526.5
30	52115.5
31	72195.0
32	100701.5
33	138085.0
34	185052.5
35	249518.5
36	327628.5
37	419263.5
38	546411.0
39	719642.0
40	933547.5
41	1179409.0
42	1444880.0
43	1705880.0
44	1938122.5
45	2113049.0
46	2222748.5
47	2256388.0
48	2199417.5
49	2075803.0
50	1909581.0
51	1724989.5
52	1548577.0
53	1378611.0
54	1196007.5
55	1011133.5
56	839247.5
57	697226.5
58	583115.0
59	480442.5
60	396652.5
61	339916.5
62	291159.5
63	238716.5
64	205408.0
65	183627.5
66	158951.0
67	133432.5
68	97346.0
69	68596.5
70	59150.0
71	51187.0
72	42188.5
73	39183.5
74	34316.0
75	24822.0
76	15387.0
77	5544.0
78	730.5
79	197.5
80	71.5
81	26.5
82	10.5
83	4.0
84	3.5
85	2.5
86	2.0
87	1.0
88	0.5
89	0.5
90	1.0
91	1.5
92	1.0
93	0.5
94	0.5
95	0.5
96	1.0
97	2.0
98	1.5
99	2.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05832933795640312
2	0.0
3	7.481440416686306E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.150683979180187E-4
12-13	0.0
14-15	0.0
16-17	0.0013970151239620005
18-19	0.0019969690958385753
20-21	0.0
22-23	0.0
24-25	1.2373151458365813E-4
26-27	0.0
28-29	2.877477083340887E-4
30-31	0.0016703754468793846
32-33	0.0
34-35	0.0
36-37	1.4387385416704433E-6
38-39	0.0028861095145909094
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.002199831230214108
48-49	0.0
50-51	0.0014761457437538748
52-53	4.920485812512916E-4
54-55	0.0
56-57	6.517485593767108E-4
58-59	1.2373151458365813E-4
60-61	0.0010445241812527418
62-63	0.0032918337833419747
64-65	0.002005601527088598
66-67	9.610773458358561E-4
68-69	3.726332822926448E-4
70-71	8.718755562522886E-4
72-73	0.0
74-75	0.0
76-77	9.481286989608222E-4
78-79	2.8774770833408867E-6
80-81	0.001758138497921282
82-83	0.0011567457875030363
84-85	2.014233958338621E-5
86-87	0.0010301367958360373
88-89	0.0
90-91	0.0020948033166721656
92-93	0.001145235879169673
94-95	0.0
96-97	2.719215843757138E-4
98-99	0.0011538683104196955
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4752666E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.929048000121504
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.85948526330317	20.699030074982407
2	15.747680054642691	10.371122248197704
3	6.701196184311467	6.6199203243427
4	3.6855600525649885	4.854479355129714
5	2.432240232049311	4.004567767448922
6	1.5451149002179871	3.052749762898863
7	1.1193391271865938	2.5801140293279015
8	0.8574592803112624	2.258825423961536
9	0.7003396498396204	2.075536615136143
>10	3.8937243638972268	23.562431325254828
>50	0.2923313814971338	6.624812630036477
>100	0.15310484708414054	9.154457115678962
>500	0.008717071977829463	1.9287011785507138
>1k	0.0036338575934706566	2.0573306474402004
>5k	7.373352308794788E-5	0.1559215016130052
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4529725000090644E-5	0.0	0.0	0.0	8.632431250022661E-6
2	5.754954166681774E-5	2.8774770833408867E-6	2.8774770833408867E-6	0.0	1.7264862500045322E-5
3	8.63243125002266E-5	2.8774770833408867E-6	5.754954166681773E-6	0.0	8.920178958356749E-5
4	1.3811890000036257E-4	2.8774770833408867E-6	1.1509908333363547E-5	0.0	9.495674375024926E-5
5	1.6977114791711233E-4	2.8774770833408867E-6	1.1509908333363547E-5	0.0	1.0646665208361281E-4
6	1.8991348750049852E-4	5.754954166681773E-6	5.179458750013596E-5	0.0	1.0646665208361281E-4
7	2.2156573541724827E-4	5.754954166681773E-6	5.4672064583476846E-5	0.0	1.0934412916695369E-4
8	2.7336032291738424E-4	8.632431250022661E-6	5.4672064583476846E-5	0.0	1.2373151458365813E-4
9	2.9062518541742954E-4	1.1509908333363547E-5	5.4672064583476846E-5	0.0	1.3524142291702166E-4
10-11	3.2659364895919066E-4	2.7336032291738426E-5	6.186575729182906E-5	0.0	1.5969997812541922E-4
12-13	3.467359885425768E-4	4.891711041679507E-5	6.33044958334995E-5	0.0	2.2732068958393004E-4
14-15	4.5320264062618967E-4	5.3233326041806404E-5	6.33044958334995E-5	0.0	2.546567218756685E-4
16-17	5.654242468764842E-4	6.042701875015862E-5	6.33044958334995E-5	0.0	2.6760536875070247E-4
18-19	6.301674812516542E-4	6.762071145851084E-5	6.618197291684039E-5	0.0	2.79115277084066E-4
20-21	7.179305322935512E-4	6.905945000018129E-5	6.905945000018129E-5	0.0	2.848702312507478E-4
22-23	8.243971843771641E-4	6.905945000018129E-5	6.905945000018129E-5	0.0	2.877477083340887E-4
24-25	9.682710385442083E-4	8.200809687521527E-5	6.905945000018129E-5	2.8774770833408867E-6	2.877477083340887E-4
26-27	0.0011020737229195596	1.1222160625029458E-4	6.905945000018129E-5	2.8774770833408867E-6	2.877477083340887E-4
28-29	0.0012934259489617286	1.1509908333363547E-4	7.049818854185173E-5	2.8774770833408867E-6	2.877477083340887E-4
30-31	0.0016286520291709417	1.1509908333363547E-4	7.769188125020394E-5	2.8774770833408867E-6	2.963801395841113E-4
32-33	0.002125016826047245	1.1653782187530592E-4	8.056935833354482E-5	4.31621562501133E-6	2.9781887812578177E-4
34-35	0.0026918798114653995	1.3236394583368078E-4	8.056935833354482E-5	5.754954166681773E-6	3.337873416675429E-4
36-37	0.0034472175458423824	1.3524142291702166E-4	8.056935833354482E-5	5.754954166681773E-6	3.5680715833426996E-4
38-39	0.004532026406261896	1.3811890000036255E-4	8.056935833354482E-5	5.754954166681773E-6	3.683170666676335E-4
40-41	0.006032630705224169	1.4675133125038523E-4	8.056935833354482E-5	5.754954166681773E-6	3.7407202083431526E-4
42-43	0.008094343035437915	1.6833240937544187E-4	8.200809687521527E-5	5.754954166681773E-6	3.769494979176562E-4
44-45	0.010274031926068636	1.8991348750049852E-4	8.63243125002266E-5	5.754954166681773E-6	4.244278697927808E-4
46-47	0.012860883823992093	2.0717835000054385E-4	8.63243125002266E-5	5.754954166681773E-6	4.517639020845192E-4
48-49	0.016336876140667884	2.0717835000054385E-4	8.63243125002266E-5	5.754954166681773E-6	5.107521822930074E-4
50-51	0.020748048509429462	2.1437204270889604E-4	8.920178958356749E-5	5.754954166681773E-6	5.625467697931433E-4
52-53	0.02648861529069453	2.2156573541724827E-4	8.920178958356749E-5	5.754954166681773E-6	5.870053250015409E-4
54-55	0.03284640090633622	2.2444321250058915E-4	9.783422083359014E-5	5.754954166681773E-6	6.057089260432567E-4
56-57	0.04007750081677187	2.6328915312569116E-4	1.2517025312532855E-4	5.754954166681773E-6	6.114638802099384E-4
58-59	0.04856030325846081	2.7767653854239555E-4	1.5826123958374876E-4	5.754954166681773E-6	6.172188343766202E-4
60-61	0.05992058278349063	2.920639239591E-4	1.5826123958374876E-4	5.754954166681773E-6	6.215350500016315E-4
62-63	0.07379433854081871	2.963801395841113E-4	1.5826123958374876E-4	5.754954166681773E-6	6.244125270849724E-4
64-65	0.09068800649711306	2.992576166674522E-4	1.5826123958374876E-4	5.754954166681773E-6	6.33044958334995E-4
66-67	0.11053396594091515	3.1364500208415667E-4	1.6113871666708965E-4	5.754954166681773E-6	6.35922435418336E-4
68-69	0.1341609302722272	3.165224791674975E-4	1.726486250004532E-4	5.754954166681773E-6	6.488710822933699E-4
70-71	0.16353277760042928	3.165224791674975E-4	1.755261020837941E-4	5.754954166681773E-6	6.531872979183813E-4
72-73	0.2004479311026095	3.1939995625083844E-4	1.7840357916713498E-4	5.754954166681773E-6	6.546260364600517E-4
74-75	0.24754503726419147	3.3666481875088374E-4	1.8415853333381675E-4	5.754954166681773E-6	6.632584677100743E-4
76-77	0.30542117257996837	3.8414319062600834E-4	1.8415853333381675E-4	5.754954166681773E-6	6.963494541684946E-4
78-79	0.3752704900395267	3.9996931458438325E-4	1.8415853333381675E-4	5.754954166681773E-6	7.10736839585199E-4
80-81	0.45919642539078875	4.014080531260537E-4	1.8703601041715763E-4	5.754954166681773E-6	7.251242250019035E-4
82-83	0.5618288392608498	4.0284679166772416E-4	1.8703601041715763E-4	5.754954166681773E-6	7.409503489602783E-4
84-85	0.6901297874528532	4.229891312511104E-4	1.8703601041715763E-4	5.754954166681773E-6	7.481440416686305E-4
86-87	0.8507994753553583	4.4744768645950785E-4	1.8991348750049852E-4	5.754954166681773E-6	7.797962895853803E-4
88-89	1.0459528486246206	4.776611958345872E-4	1.9710718020885074E-4	5.754954166681773E-6	8.028161062521074E-4
90	1.2117832916760976	4.978035354179734E-4	2.0142339583386208E-4	5.754954166681773E-6	8.1144853750213E-4
>>END_MODULE
