##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654138_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	38008707
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.623020193767708	32.0	32.0	32.0	32.0	32.0
2	31.695758132472122	32.0	32.0	32.0	32.0	32.0
3	35.757964063339486	37.0	37.0	37.0	32.0	37.0
4	36.36907075002578	37.0	37.0	37.0	37.0	37.0
5	36.44475693424667	37.0	37.0	37.0	37.0	37.0
6	40.10729060054582	41.0	41.0	41.0	37.0	41.0
7	40.12186376137446	41.0	41.0	41.0	37.0	41.0
8	40.25808241779969	41.0	41.0	41.0	41.0	41.0
9	40.23256381754844	41.0	41.0	41.0	41.0	41.0
10-11	40.297210070839824	41.0	41.0	41.0	41.0	41.0
12-13	40.25714968415001	41.0	41.0	41.0	41.0	41.0
14-15	40.28969224078051	41.0	41.0	41.0	41.0	41.0
16-17	40.29176993576761	41.0	41.0	41.0	41.0	41.0
18-19	40.28891718152896	41.0	41.0	41.0	41.0	41.0
20-21	40.220894820231585	41.0	41.0	41.0	41.0	41.0
22-23	40.17958485143944	41.0	41.0	41.0	41.0	41.0
24-25	40.21593871898878	41.0	41.0	41.0	41.0	41.0
26-27	40.192334364333945	41.0	41.0	41.0	41.0	41.0
28-29	40.11129094709799	41.0	41.0	41.0	41.0	41.0
30-31	40.124607711596184	41.0	41.0	41.0	41.0	41.0
32-33	40.138910158138245	41.0	41.0	41.0	41.0	41.0
34-35	40.13556062299094	41.0	41.0	41.0	41.0	41.0
36-37	40.11829340840245	41.0	41.0	41.0	41.0	41.0
38-39	40.09648406350682	41.0	41.0	41.0	37.0	41.0
40-41	40.08436355122525	41.0	41.0	41.0	37.0	41.0
42-43	40.03380272841168	41.0	41.0	41.0	37.0	41.0
44-45	40.0498633115828	41.0	41.0	41.0	37.0	41.0
46-47	40.03656437194772	41.0	41.0	41.0	37.0	41.0
48-49	40.014526816184514	41.0	41.0	41.0	37.0	41.0
50-51	39.948343217779026	41.0	41.0	41.0	37.0	41.0
52-53	39.97074123042386	41.0	41.0	41.0	37.0	41.0
54-55	39.91782432904124	41.0	41.0	41.0	37.0	41.0
56-57	39.91857445979417	41.0	41.0	41.0	37.0	41.0
58-59	39.910141747258066	41.0	41.0	41.0	37.0	41.0
60-61	39.84018507917146	41.0	41.0	41.0	37.0	41.0
62-63	39.800997295172394	41.0	41.0	41.0	37.0	41.0
64-65	39.79470626296232	41.0	41.0	41.0	37.0	41.0
66-67	39.76335467028647	41.0	41.0	41.0	37.0	41.0
68-69	39.68400533856624	41.0	41.0	41.0	37.0	41.0
70-71	39.70671581908851	41.0	41.0	41.0	37.0	41.0
72-73	39.62560482523123	41.0	41.0	41.0	37.0	41.0
74-75	39.665781382671085	41.0	41.0	41.0	37.0	41.0
76-77	39.13637256852752	41.0	39.0	41.0	34.5	41.0
78-79	39.44546885007164	41.0	41.0	41.0	37.0	41.0
80-81	39.646610801572386	41.0	41.0	41.0	37.0	41.0
82-83	39.787291198829784	41.0	41.0	41.0	37.0	41.0
84-85	39.74759137426064	41.0	41.0	41.0	37.0	41.0
86-87	39.7692987688321	41.0	41.0	41.0	37.0	41.0
88-89	39.70589507293684	41.0	41.0	41.0	37.0	41.0
90-91	39.72637086812766	41.0	41.0	41.0	37.0	41.0
92-93	39.68317913313915	41.0	41.0	41.0	37.0	41.0
94-95	39.54392259384146	41.0	41.0	41.0	37.0	41.0
96-97	39.51264026161164	41.0	41.0	41.0	37.0	41.0
98-99	39.567437547928165	41.0	41.0	41.0	37.0	41.0
100-101	39.04152527472192	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	1.0
16	8.0
17	26.0
18	67.0
19	185.0
20	569.0
21	1854.0
22	4290.0
23	8986.0
24	16974.0
25	30076.0
26	50255.0
27	78752.0
28	115934.0
29	161738.0
30	211618.0
31	270038.0
32	333512.0
33	405325.0
34	489766.0
35	600962.0
36	761948.0
37	1036146.0
38	1635889.0
39	3692011.0
40	2.8101775E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.75446101358907	11.659278107906426	6.0654356651112264	44.52082521339327
2	23.97367792595523	13.584400542749323	32.27214490616584	30.169776625129607
3	20.636966156202053	15.820175098300501	21.627683888325905	41.91517485717154
4	23.791592357867877	23.57156849133604	20.97279116426761	31.66404798652847
5	24.764933466429152	28.25064267511126	23.510428807799226	23.473995050660363
6	20.382877007628807	31.70180979847591	24.93655466890784	22.978758524987445
7	15.955594069537804	25.41729188525145	39.83192324853355	18.795190796677193
8	18.607433817730236	24.479588321696923	31.597930968817224	25.315046891755618
9	18.67464831396107	22.687078113370365	34.08365774791961	24.55461582474895
10-11	21.275424050618007	31.251913199353172	24.87440091675968	22.59826183326914
12-13	21.734269564886226	25.149901478101444	27.457754834683367	25.658074122328962
14-15	21.154247630681045	26.08189355139074	27.42616316834982	25.337695649578396
16-17	21.706325882857644	26.4358383507468	26.785041619178784	25.072794147216776
18-19	21.49743097521386	26.696608684170815	26.6005490561124	25.205411284502922
20-21	21.68208583483478	26.623980613645237	26.73763781736757	24.95629573415241
22-23	21.66047505904371	26.82550737650718	26.693274517336253	24.820743047112863
24-25	21.65107458141104	26.517533206272976	26.54081339836159	25.29057881395439
26-27	21.47412959877851	26.667148924587202	26.769478109318477	25.08924336731581
28-29	21.515012495712487	26.896999752977198	26.6051937089983	24.982794042312015
30-31	21.62408602849868	26.54779600895132	26.620972662921684	25.20714529962832
32-33	21.68831585866563	26.52625839977814	26.745808673288874	25.039617068267354
34-35	21.803064767414106	26.69275574597848	26.454055167034234	25.05012431957318
36-37	21.62954021314352	26.513362723899515	26.432609878909407	25.424487184047557
38-39	21.67126194769999	26.543865244567687	26.690572020926822	25.0943007868055
40-41	21.789471443725482	26.851947029898714	26.517693178930386	24.840888347445414
42-43	21.8016098258749	26.486036475800134	26.524369797688724	25.18798390063624
44-45	21.823561507367494	26.49330379060367	26.665898753549666	25.017235948479165
46-47	21.809682838582937	26.6223560245941	26.506792786806006	25.06116835001696
48-49	21.6890579001465	26.440882777486152	26.513024509627297	25.357034812740046
50-51	21.68494145608775	26.457155200936082	26.702937142679588	25.15496620029658
52-53	22.05566740273485	26.597058405591117	26.35959439504217	24.987679796631863
54-55	21.892156101302387	26.454209633547833	26.36602899615937	25.28760526899041
56-57	21.80408885690548	26.361977851140427	26.744504502646222	25.08942878930787
58-59	21.888229959078554	26.471816832643874	26.63234437032288	25.00760883795469
60-61	21.86687352470359	26.326419872221013	26.553646943051202	25.253059660024196
62-63	21.837200289371356	26.31914127748906	26.70388578774127	25.139772645398313
64-65	21.967139575210428	26.48812473151125	26.55216671859669	24.99256897468163
66-67	21.85435623706199	26.303044416514886	26.541752537467616	25.30084680895551
68-69	21.890822819261306	26.319035884603487	26.740007478121576	25.05013381801363
70-71	22.095235030507798	26.556901283724056	26.50909355847769	24.838770127290452
72-73	22.067969287154245	26.35892308294198	26.40350394613452	25.169603683769253
74-75	22.00089478023508	26.3309573163202	26.645755047352853	25.022392856091862
76-77	22.002847926397497	26.507392108866	26.53005665254543	24.959703312191074
78-79	21.923069303265514	26.316274202764987	26.519623694975998	25.2410327989935
80-81	22.023422595232837	26.318149182826957	26.6396065312168	25.01882169072341
82-83	22.10844237565405	26.582330820445343	26.42047181595913	24.888754987941482
84-85	22.140667858035464	26.358548563989814	26.36679142200259	25.133992155972134
86-87	22.12209267140326	26.331320606175744	26.568914153520527	24.977672568900473
88-89	22.289634371338416	26.43462033440024	26.471148866264503	24.80459642799684
90-91	22.15348919517885	26.279362093849667	26.464638303120974	25.102510407850502
92-93	22.161252905191837	26.25784586787745	26.467543905518408	25.113357321412305
94-95	22.39848924555533	26.41797294042954	26.27580883578842	24.90772897822671
96-97	22.327134476215456	26.360887767122904	26.1445654134153	25.167412343246344
98-99	22.340083965551607	26.437981535397938	26.353776827161596	24.868157671888863
100-101	22.394181720313377	26.635208353641605	26.22640799207858	24.744201933966437
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	38.0
1	31.5
2	20.0
3	20.0
4	31.0
5	40.0
6	53.0
7	66.5
8	85.5
9	93.5
10	119.0
11	165.5
12	188.0
13	231.5
14	280.0
15	324.5
16	404.5
17	492.0
18	602.5
19	775.0
20	1010.5
21	1431.0
22	2118.5
23	3038.5
24	4630.0
25	6977.0
26	10267.0
27	15659.0
28	23048.0
29	33426.0
30	48604.0
31	68518.5
32	96690.5
33	135453.0
34	186692.5
35	260562.0
36	359420.0
37	489842.5
38	669959.5
39	907660.0
40	1176183.0
41	1458958.0
42	1751000.5
43	2025218.0
44	2259260.5
45	2413465.0
46	2487212.0
47	2484989.5
48	2390124.5
49	2235577.5
50	2050420.5
51	1836921.0
52	1643810.0
53	1460529.5
54	1260380.0
55	1072290.5
56	892851.5
57	719359.5
58	570417.5
59	456350.0
60	368733.0
61	311124.0
62	266569.0
63	221420.0
64	184230.5
65	156353.5
66	134591.0
67	109853.5
68	77591.0
69	52674.0
70	39797.0
71	31030.5
72	24566.5
73	21082.0
74	16426.0
75	10172.5
76	5617.5
77	2062.0
78	276.5
79	82.5
80	31.0
81	18.5
82	7.5
83	1.0
84	2.5
85	3.5
86	2.5
87	2.5
88	3.0
89	2.5
90	2.5
91	2.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03486043342647778
2	0.0
3	0.0
4	0.019403448793982914
5	0.0
6	0.0
7	0.0
8	0.0
9	0.002667809773165922
10-11	0.0035873359227926375
12-13	0.0017351287430009128
14-15	0.0
16-17	8.642756513658831E-4
18-19	6.182793852997946E-5
20-21	3.091396926498973E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	1.8416832753610904E-4
30-31	0.0
32-33	0.002061369780350592
34-35	5.551359587159858E-4
36-37	3.6570567896455935E-4
38-39	0.004212192748361579
40-41	5.2619522153174E-5
42-43	0.0
44-45	0.002459962660660885
46-47	2.10478088612696E-4
48-49	5.090938768319586E-4
50-51	4.078012966870986E-5
52-53	0.0
54-55	9.550443270801082E-4
56-57	2.986157882192625E-4
58-59	7.235184296061427E-4
60-61	8.287574739124906E-5
62-63	2.828299315733103E-4
64-65	0.002182394681302892
66-67	0.002061369780350592
68-69	0.008349402677654886
70-71	0.008795353127903036
72-73	6.472201224840403E-4
74-75	0.0012931247569142512
76-77	0.0
78-79	0.0013102261016140328
80-81	0.00882429386508728
82-83	0.005713164617780868
84-85	1.1970941289847087E-4
86-87	0.010226604130469368
88-89	1.6312051867483943E-4
90-91	0.002260008476478824
92-93	6.432736583225523E-4
94-95	0.014320402953986306
96-97	0.0010089793372871116
98-99	3.775450714490235E-4
100-101	0.015595110878146946
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.8008707E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.806562517115406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.37012116551283	19.83779158050946
2	15.515683228904875	9.870010972318436
3	6.640713116300831	6.3365477067555185
4	3.8311130640519275	4.874181487276207
5	2.4366514891420703	3.8750753960909807
6	1.6320722680948985	3.114636517656643
7	1.2050835292865636	2.6830695228819406
8	0.8972537643770246	2.2830846360299972
9	0.713618063824484	2.0427963804338667
>10	4.283804582546724	25.13093575447836
>50	0.30550922521225654	6.69646701450911
>100	0.15519744596759472	8.92479445715444
>500	0.009032916463973712	1.9594581849769446
>1k	0.004093534313520878	2.2609161274573384
>5k	5.260600041397421E-5	0.11023426147069146
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.735756993785661E-5	0.0	0.0	0.0	1.0523904430634802E-5
2	7.103635490678491E-5	0.0	0.0	2.6309761076587004E-6	1.3154880538293502E-5
3	1.1313197262932411E-4	0.0	0.0	5.261952215317401E-6	1.8416832753610902E-5
4	1.8679930364376773E-4	0.0	5.261952215317401E-6	7.8929283229761E-6	2.1047808861269603E-5
5	2.2363296915098953E-4	0.0	5.261952215317401E-6	7.8929283229761E-6	2.3678784968928304E-5
6	2.815144435194809E-4	0.0	1.57858566459522E-5	7.8929283229761E-6	2.6309761076587005E-5
7	3.0782420459606795E-4	0.0	1.8416832753610902E-5	7.8929283229761E-6	2.6309761076587005E-5
8	3.6044372674924195E-4	0.0	1.8416832753610902E-5	7.8929283229761E-6	2.6309761076587005E-5
9	3.86753487825829E-4	0.0	2.6309761076587005E-5	7.8929283229761E-6	3.15717132919044E-5
10-11	4.5384337857112583E-4	2.6309761076587004E-6	2.6309761076587005E-5	7.8929283229761E-6	3.6833665507221805E-5
12-13	5.301416856932281E-4	1.973232080744025E-5	2.6309761076587005E-5	7.8929283229761E-6	5.1304034099344654E-5
14-15	6.643214671838218E-4	2.7625249130416357E-5	2.6309761076587005E-5	7.8929283229761E-6	5.261952215317401E-5
16-17	7.590366070595351E-4	4.2095617722539206E-5	2.6309761076587005E-5	7.8929283229761E-6	5.261952215317401E-5
18-19	8.734840677426885E-4	4.4726593830197904E-5	2.6309761076587005E-5	7.8929283229761E-6	5.5250498260832714E-5
20-21	9.77407623995207E-4	4.4726593830197904E-5	2.8940737184245706E-5	7.8929283229761E-6	6.182793852997946E-5
22-23	0.0010694917877632618	4.9988546045515305E-5	2.8940737184245706E-5	7.8929283229761E-6	6.31434265838088E-5
24-25	0.0011852547365002446	5.788147436849141E-5	2.8940737184245706E-5	7.8929283229761E-6	6.31434265838088E-5
26-27	0.0013628456237672068	7.498281906827297E-5	2.8940737184245706E-5	9.208416376805451E-6	6.840537879912621E-5
28-29	0.0016233122584254182	7.892928322976102E-5	3.15717132919044E-5	1.0523904430634802E-5	6.972086685295556E-5
30-31	0.001998226353766783	8.156025933741972E-5	3.15717132919044E-5	1.0523904430634802E-5	7.62983071221023E-5
32-33	0.0024967963261681063	8.945318766039581E-5	3.15717132919044E-5	1.0523904430634802E-5	7.892928322976102E-5
34-35	0.003190058530536174	9.076867571422516E-5	3.15717132919044E-5	1.0523904430634802E-5	8.156025933741972E-5
36-37	0.004241133485545825	9.339965182188387E-5	3.15717132919044E-5	1.0523904430634802E-5	8.813769960656646E-5
38-39	0.005836820494840827	9.471513987571322E-5	3.15717132919044E-5	1.0523904430634802E-5	8.945318766039581E-5
40-41	0.007904767715460566	9.603062792954256E-5	3.15717132919044E-5	1.0523904430634802E-5	9.208416376805452E-5
42-43	0.010404195017736331	1.1050099652166541E-4	3.15717132919044E-5	1.0523904430634802E-5	9.734611598337191E-5
44-45	0.013598200012433993	1.262868531676176E-4	3.15717132919044E-5	1.0523904430634802E-5	9.734611598337191E-5
46-47	0.017184220447172802	1.4601917397505788E-4	3.15717132919044E-5	1.0523904430634802E-5	1.0523904430634802E-4
48-49	0.02188182828739741	1.4733466202888722E-4	3.288720134573376E-5	1.0523904430634802E-5	1.0655453236017737E-4
50-51	0.027965960536358157	1.5128112619037528E-4	3.420268939956311E-5	1.0523904430634802E-5	1.0787002041400672E-4
52-53	0.035581320879976265	1.525966142442046E-4	3.420268939956311E-5	1.0523904430634802E-5	1.0787002041400672E-4
54-55	0.04397018819924603	1.525966142442046E-4	3.420268939956311E-5	1.0523904430634802E-5	1.1839392484464152E-4
56-57	0.05367059710818366	1.8022186337462098E-4	3.5518177453392456E-5	1.0523904430634802E-5	1.394417337059111E-4
58-59	0.06518901050751344	1.8416832753610904E-4	4.4726593830197904E-5	1.0523904430634802E-5	1.5654307840569267E-4
60-61	0.07985933328381836	1.8943027975142643E-4	4.4726593830197904E-5	1.0523904430634802E-5	1.6180503062101007E-4
62-63	0.09776312569643582	1.9074576780525577E-4	4.4726593830197904E-5	1.0523904430634802E-5	1.6575149478249813E-4
64-65	0.11940290418192863	1.9206125585908513E-4	4.4726593830197904E-5	1.0523904430634802E-5	1.6706698283632746E-4
66-67	0.14390650016060794	1.9206125585908513E-4	4.735756993785661E-5	1.0523904430634802E-5	1.7101344699781552E-4
68-69	0.17350761234787598	1.933767439129145E-4	4.9988546045515305E-5	1.0523904430634802E-5	1.7364442310547422E-4
70-71	0.20952962172588507	1.9600772002057316E-4	4.9988546045515305E-5	1.1839392484464152E-5	1.7890637532079161E-4
72-73	0.25307096081958275	1.9995418418206122E-4	4.9988546045515305E-5	1.3154880538293502E-5	1.815373514284503E-4
74-75	0.3071901393541222	2.10478088612696E-4	5.261952215317401E-5	1.3154880538293502E-5	1.8548381558993837E-4
76-77	0.3724672875612422	2.3678784968928304E-4	5.261952215317401E-5	1.3154880538293502E-5	1.881147916975971E-4
78-79	0.45048362208164566	2.3678784968928304E-4	5.261952215317401E-5	1.3154880538293502E-5	1.8943027975142643E-4
80-81	0.5437517250981466	2.3678784968928304E-4	5.261952215317401E-5	1.3154880538293502E-5	1.9206125585908513E-4
82-83	0.656541670833475	2.3678784968928304E-4	5.393501020700336E-5	1.3154880538293502E-5	1.9469223196674383E-4
84-85	0.7947507922329481	2.4731175411991783E-4	5.525049826083271E-5	1.3154880538293502E-5	2.0916260055886668E-4
86-87	0.9634963378259618	2.525737063352352E-4	5.788147436849141E-5	1.3154880538293502E-5	2.1310906472035474E-4
88-89	1.162971684356429	2.696750510350168E-4	5.788147436849141E-5	1.3154880538293502E-5	2.1837101693567213E-4
90	1.3332550354843693	2.815144435194809E-4	5.788147436849141E-5	1.3154880538293502E-5	2.2100199304333083E-4
>>END_MODULE
