##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654126_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29541717
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.621807865805497	32.0	32.0	32.0	32.0	32.0
2	31.677368109646437	32.0	32.0	32.0	32.0	32.0
3	35.70954284749258	37.0	37.0	37.0	32.0	37.0
4	36.347136288659186	37.0	37.0	37.0	37.0	37.0
5	36.42295094086779	37.0	37.0	37.0	37.0	37.0
6	40.06094479884158	41.0	41.0	41.0	37.0	41.0
7	40.071513717364496	41.0	41.0	41.0	37.0	41.0
8	40.21578627268009	41.0	41.0	41.0	37.0	41.0
9	40.19209834689026	41.0	41.0	41.0	37.0	41.0
10-11	40.26036518121137	41.0	41.0	41.0	41.0	41.0
12-13	40.22851071926523	41.0	41.0	41.0	41.0	41.0
14-15	40.25248896331922	41.0	41.0	41.0	41.0	41.0
16-17	40.249921577002446	41.0	41.0	41.0	41.0	41.0
18-19	40.239138300593694	41.0	41.0	41.0	41.0	41.0
20-21	40.16591058332865	41.0	41.0	41.0	39.0	41.0
22-23	40.114986782928014	41.0	41.0	41.0	37.0	41.0
24-25	40.14743388138204	41.0	41.0	41.0	37.0	41.0
26-27	40.10640395411005	41.0	41.0	41.0	37.0	41.0
28-29	40.009314624468175	41.0	41.0	41.0	37.0	41.0
30-31	40.009398268895474	41.0	41.0	41.0	37.0	41.0
32-33	40.01280438438971	41.0	41.0	41.0	37.0	41.0
34-35	39.98785014764037	41.0	41.0	41.0	37.0	41.0
36-37	39.95157126445967	41.0	41.0	41.0	37.0	41.0
38-39	39.908640838310106	41.0	41.0	41.0	37.0	41.0
40-41	39.87045116571932	41.0	41.0	41.0	37.0	41.0
42-43	39.78850880603859	41.0	41.0	41.0	37.0	41.0
44-45	39.789685142539284	41.0	41.0	41.0	37.0	41.0
46-47	39.75086263604787	41.0	41.0	41.0	37.0	41.0
48-49	39.7014869853367	41.0	41.0	41.0	37.0	41.0
50-51	39.59634913908356	41.0	41.0	41.0	37.0	41.0
52-53	39.571197215788104	41.0	41.0	41.0	37.0	41.0
54-55	39.47850226511885	41.0	41.0	41.0	37.0	41.0
56-57	39.43689044885238	41.0	41.0	41.0	37.0	41.0
58-59	39.379204566884184	41.0	41.0	41.0	37.0	41.0
60-61	39.23996110314103	41.0	41.0	41.0	37.0	41.0
62-63	39.1113197821237	41.0	41.0	41.0	37.0	41.0
64-65	39.00266548488025	41.0	41.0	41.0	37.0	41.0
66-67	38.88873376926602	41.0	41.0	41.0	32.0	41.0
68-69	38.68788515237621	41.0	41.0	41.0	32.0	41.0
70-71	38.60662860252842	41.0	41.0	41.0	32.0	41.0
72-73	38.37844398143818	41.0	37.0	41.0	32.0	41.0
74-75	38.312232884094044	41.0	37.0	41.0	32.0	41.0
76-77	36.59386620960454	39.0	34.5	41.0	27.0	41.0
78-79	37.331495610089284	41.0	37.0	41.0	29.5	41.0
80-81	38.0675401162363	41.0	37.0	41.0	32.0	41.0
82-83	38.277144622298025	41.0	37.0	41.0	32.0	41.0
84-85	38.2108904502741	41.0	37.0	41.0	32.0	41.0
86-87	38.1894994627428	41.0	37.0	41.0	32.0	41.0
88-89	38.081733620967256	41.0	37.0	41.0	32.0	41.0
90-91	38.01554466180825	41.0	37.0	41.0	32.0	41.0
92-93	37.962169632861894	41.0	37.0	41.0	32.0	41.0
94-95	37.803693011479325	41.0	37.0	41.0	32.0	41.0
96-97	37.72353191251544	41.0	37.0	41.0	29.5	41.0
98-99	37.80267829388522	41.0	37.0	41.0	32.0	41.0
100-101	36.89102732248095	41.0	34.5	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	1.0
15	4.0
16	7.0
17	15.0
18	53.0
19	212.0
20	765.0
21	2632.0
22	6663.0
23	14023.0
24	26899.0
25	46674.0
26	74804.0
27	112211.0
28	156076.0
29	204706.0
30	258546.0
31	316906.0
32	383375.0
33	469274.0
34	559896.0
35	696325.0
36	920696.0
37	1368373.0
38	2454287.0
39	6661925.0
40	1.4806366E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.406401351217276	11.617593726646009	6.333404330031027	51.642600592105694
2	21.647255641911404	12.86851742571361	35.157364076028486	30.326862856346505
3	19.688716806812547	13.95621994483259	20.99748636817555	45.35757688017931
4	23.056553932267267	21.034475858800246	20.539249710886725	35.36972049804576
5	24.70477596139723	26.352029572282476	24.27016344378358	24.673031022536705
6	20.36288547480162	30.664334777832984	25.618162275401936	23.35461747196346
7	15.931172856337362	26.070820460435662	39.09545609688157	18.902550586345406
8	17.72873255809742	24.729517245053835	33.100486339368835	24.441263857479917
9	18.017915732201562	22.597766274655086	35.39331011675855	23.991007876384806
10-11	20.451228575134355	31.20149690404303	25.959377509090885	22.387897011731727
12-13	21.132303736011224	25.481390404741333	27.802189519862676	25.58411633938477
14-15	20.734977929684995	26.209536162031476	27.55027915269786	25.505206755585668
16-17	21.471687785551776	26.365012394432373	26.862013392012386	25.30128642800346
18-19	21.095213675355442	26.56089673116776	26.710096016320524	25.633793577156272
20-21	21.46980815949726	26.55390746290725	26.921365257373797	25.05491912022169
22-23	21.262699456500787	26.73785345652049	26.96416054625396	25.035286540724766
24-25	21.189459319332375	26.355980364241866	26.870234503807524	25.584325812618236
26-27	21.11240013571317	26.580987151153064	27.051914416484323	25.254698296649448
28-29	21.176428056066666	26.80089149376828	26.82562775303014	25.197052697134914
30-31	21.21500757725084	26.415627771398665	26.78885929345271	25.58050535789778
32-33	21.440804253703803	26.38078690703936	26.877213481977634	25.301195357279205
34-35	21.549380916115652	26.517396478194748	26.619261464715095	25.313961140974506
36-37	21.31861479676407	26.335837196711097	26.536938257047527	25.808609749477302
38-39	21.427320722617736	26.427837048370893	26.8515643773246	25.293277851686767
40-41	21.539192421884866	26.73239736935243	26.602819516189314	25.12559069257339
42-43	21.487721922188882	26.241472355855283	26.70389977671237	25.56690594524347
44-45	21.50768929441031	26.264566742765403	26.94149018007409	25.286253782750194
46-47	21.545854255345834	26.387397820968246	26.695789501014044	25.37095842267188
48-49	21.415635542082263	26.183441995174768	26.690124569367455	25.710797893375513
50-51	21.464426308501828	26.204782357687208	26.85558094903459	25.47521038477637
52-53	21.85748038951155	26.391038814703965	26.414532709794763	25.336948085989718
54-55	21.615696141137544	26.190628679998536	26.497186055715883	25.69648912314804
56-57	21.547014682634504	26.179842529355096	26.97732552564037	25.295817262370022
58-59	21.59659115643159	26.292418526687563	26.765691494349277	25.345298822531575
60-61	21.619308033891425	26.011661315613516	26.694734907284	25.674295743211058
62-63	21.58889131051856	26.031626791892887	26.97127871801118	25.408203179577377
64-65	21.747301881451957	26.27801078934296	26.6634399751167	25.311247354088383
66-67	21.545674114467463	26.15534442962274	26.641771212830623	25.65721024307917
68-69	21.644089538689943	26.187723320095884	26.843949149433904	25.324237991780272
70-71	21.838820567304424	26.37730401799007	26.61524728280837	25.16862813189714
72-73	21.815770605060365	26.21613607707131	26.440371296355114	25.52772202151321
74-75	21.804547776020826	26.236088467483516	26.735974009323364	25.22338974717229
76-77	21.71124650608494	26.386812587772063	26.611303601615305	25.290637304527696
78-79	21.643655974982853	26.118811807453557	26.66132782060665	25.576204396956943
80-81	21.79433621731259	26.14071698646302	26.783802235696385	25.281144560528002
82-83	21.83786372294394	26.461314401057734	26.480941957510574	25.219879918487752
84-85	21.91120334311313	26.12940022906275	26.47127813775967	25.48811829006445
86-87	21.87176426229772	26.165324292106007	26.71482259962651	25.248088845969768
88-89	22.051358700932113	26.207880095081954	26.611956722560816	25.12880448142512
90-91	21.902096753525225	26.02203335637075	26.586264713772167	25.489605176331864
92-93	21.885960633438	26.079120240198893	26.673234936674543	25.36168418968856
94-95	22.105478601316854	26.243062573914234	26.417904060761582	25.233554764007327
96-97	22.025230611829848	26.15529330076798	26.28089154561853	25.538584541783642
98-99	22.01917474819038	26.32057995071935	26.596834326601694	25.06341097448858
100-101	22.04049460856433	26.516991310216913	26.43394184475612	25.00857223646264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	31.0
1	23.0
2	11.5
3	15.5
4	39.0
5	67.0
6	76.5
7	104.5
8	151.5
9	181.5
10	212.5
11	255.0
12	299.0
13	356.5
14	415.0
15	481.5
16	603.5
17	720.0
18	887.0
19	1168.0
20	1543.5
21	2079.5
22	2836.0
23	3930.0
24	5798.0
25	8451.5
26	12032.5
27	17399.5
28	24920.5
29	35682.0
30	50363.5
31	69778.5
32	96226.5
33	129940.0
34	173536.0
35	232497.0
36	304742.5
37	390995.5
38	508102.0
39	670370.0
40	860465.5
41	1068589.0
42	1293803.5
43	1520362.0
44	1720207.0
45	1852468.5
46	1925464.5
47	1936949.5
48	1864720.0
49	1742340.0
50	1599789.5
51	1436243.5
52	1292763.5
53	1156768.5
54	998466.0
55	847059.5
56	709817.5
57	571566.0
58	444172.0
59	352940.5
60	285453.0
61	239750.0
62	204731.5
63	170735.0
64	147668.5
65	130068.5
66	112630.0
67	93820.5
68	65459.0
69	42598.5
70	32221.0
71	23045.0
72	16242.5
73	13886.5
74	10752.0
75	6202.0
76	3105.0
77	935.0
78	94.0
79	18.0
80	7.0
81	6.5
82	6.5
83	3.0
84	1.0
85	0.5
86	0.0
87	1.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.5
93	1.5
94	1.5
95	1.5
96	1.0
97	0.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033264823435956686
2	0.0
3	0.0
4	0.019348909205243556
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0025489378291722177
10-11	0.003427356642811249
12-13	0.00166374892833751
14-15	0.0
16-17	7.684048967092873E-4
18-19	6.939339375568456E-5
20-21	3.1480905459895913E-4
22-23	0.0
24-25	1.6925217989191351E-6
26-27	0.0
28-29	2.149502684627302E-4
30-31	0.0
32-33	0.002000560766322418
34-35	5.517621064476382E-4
36-37	3.4696696877842276E-4
38-39	0.004256692324281625
40-41	5.923826296216973E-5
42-43	0.0
44-45	0.002403380954465172
46-47	2.166427902616493E-4
48-49	4.891387998876301E-4
50-51	4.0620523174059245E-5
52-53	0.0
54-55	8.970365534271417E-4
56-57	2.843436622184147E-4
58-59	6.871638503611689E-4
60-61	8.293356814703762E-5
62-63	2.928062712130104E-4
64-65	0.0021071896396543236
66-67	0.001965017808545116
68-69	0.008190112984969695
70-71	0.008572622911525421
72-73	6.550059361817054E-4
74-75	0.001199997955433667
76-77	0.0
78-79	0.001276161436385028
80-81	0.008645401348878944
82-83	0.005470230454106645
84-85	1.0324382973406726E-4
86-87	0.01000111130981317
88-89	1.4555687470704562E-4
90-91	0.002178275555208927
92-93	6.736236759698159E-4
94-95	0.0138956039691261
96-97	0.0010188981229493194
98-99	3.50352012376261E-4
100-101	0.015236081233870056
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.9541717E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.89975419810346
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.530920964508184	20.57251929513354
2	16.099139174057246	10.593154432550834
3	6.8617017285863415	6.7724490075357675
4	3.5771222224381103	4.707457674191496
5	2.3153681039232996	3.8087520748602715
6	1.6100496019957347	3.178214169144839
7	1.156415264173601	2.6632044559572443
8	0.8430131659199446	2.218794075562495
9	0.6622900772582682	1.9610262674675971
>10	3.8963227650972376	23.72994901540599
>50	0.2862372778384126	6.467327196140891
>100	0.14881333876968927	8.985362019799547
>500	0.008686447378157366	1.926693260864616
>1k	0.0038338675716228228	2.1746480134266335
>5k	6.450036291497526E-5	0.1255862240887605
>10k+	2.1500120971658422E-5	0.11486281786957318
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.0310261587029622E-5	0.0	3.3850435978382707E-6	0.0	0.0
2	3.723547957622098E-5	0.0	3.3850435978382707E-6	0.0	3.3850435978382707E-6
3	6.093078476108887E-5	0.0	3.3850435978382707E-6	0.0	6.770087195676541E-6
4	1.0155130793514811E-4	0.0	6.770087195676541E-6	3.3850435978382707E-6	1.3540174391353083E-5
5	1.3201670031569255E-4	0.0	6.770087195676541E-6	3.3850435978382707E-6	1.3540174391353083E-5
6	2.0310261587029623E-4	0.0	2.7080348782706165E-5	3.3850435978382707E-6	1.3540174391353083E-5
7	2.3695305184867895E-4	0.0	3.0465392380544435E-5	3.3850435978382707E-6	1.3540174391353083E-5
8	2.7757357502273817E-4	1.0155130793514811E-5	3.0465392380544435E-5	3.3850435978382707E-6	1.6925217989191352E-5
9	3.0465392380544436E-4	1.3540174391353083E-5	3.0465392380544435E-5	3.3850435978382707E-6	1.6925217989191352E-5
10-11	3.418894033816653E-4	2.3695305184867892E-5	3.0465392380544435E-5	3.3850435978382707E-6	2.0310261587029622E-5
12-13	3.774323611589672E-4	3.3850435978382705E-5	3.0465392380544435E-5	5.077565396757406E-6	2.3695305184867892E-5
14-15	4.315930587243795E-4	3.723547957622098E-5	3.0465392380544435E-5	8.462608994595676E-6	2.3695305184867892E-5
16-17	4.7390610369735784E-4	3.892800137514011E-5	3.0465392380544435E-5	1.0155130793514811E-5	2.538782698378703E-5
18-19	5.246817576649319E-4	4.2313044972978385E-5	3.0465392380544435E-5	1.0155130793514811E-5	3.0465392380544435E-5
20-21	5.822274988281826E-4	4.400556677189752E-5	3.0465392380544435E-5	1.0155130793514811E-5	3.554295777730184E-5
22-23	6.600835015784627E-4	4.400556677189752E-5	3.0465392380544435E-5	1.0155130793514811E-5	4.0620523174059245E-5
24-25	7.413245479265813E-4	5.5853219364331464E-5	3.0465392380544435E-5	1.0155130793514811E-5	4.0620523174059245E-5
26-27	8.445683776606485E-4	9.308869894055244E-5	3.0465392380544435E-5	1.0155130793514811E-5	4.0620523174059245E-5
28-29	9.63044903584988E-4	9.478122073947157E-5	3.0465392380544435E-5	1.0155130793514811E-5	4.2313044972978385E-5
30-31	0.0011729176066509607	9.478122073947157E-5	3.0465392380544435E-5	1.0155130793514811E-5	4.569808857081665E-5
32-33	0.001501266835641273	9.816626433730983E-5	3.215791417946357E-5	1.0155130793514811E-5	4.7390610369735784E-5
34-35	0.0018939318929905124	1.0324382973406725E-4	3.3850435978382705E-5	1.0155130793514811E-5	5.077565396757406E-5
36-37	0.0023644529530900318	1.1001391692974378E-4	3.3850435978382705E-5	1.3540174391353083E-5	5.077565396757406E-5
38-39	0.003117625153609047	1.150914823265012E-4	3.3850435978382705E-5	1.3540174391353083E-5	5.416069756541233E-5
40-41	0.004165296147139992	1.1678400412542034E-4	3.3850435978382705E-5	1.3540174391353083E-5	5.5853219364331464E-5
42-43	0.00546007532331313	1.5232696190272218E-4	3.3850435978382705E-5	1.3540174391353083E-5	5.75457411632506E-5
44-45	0.0068953338087965575	1.7263722348975178E-4	3.3850435978382705E-5	1.3540174391353083E-5	5.75457411632506E-5
46-47	0.008574315433324339	1.963325286746197E-4	3.3850435978382705E-5	1.3540174391353083E-5	5.75457411632506E-5
48-49	0.010786441424511649	1.963325286746197E-4	3.3850435978382705E-5	1.3540174391353083E-5	6.093078476108887E-5
50-51	0.013717889180239591	1.9802505047353882E-4	3.3850435978382705E-5	1.3540174391353083E-5	7.447095915244196E-5
52-53	0.01739743157108979	2.0818018126705364E-4	3.3850435978382705E-5	1.3540174391353083E-5	7.447095915244196E-5
54-55	0.02168628180955088	2.115652248648919E-4	3.892800137514011E-5	1.3540174391353083E-5	7.447095915244196E-5
56-57	0.026821392947471538	2.3864557364759807E-4	5.077565396757406E-5	1.3540174391353083E-5	7.785600275028022E-5
58-59	0.03260981749977498	2.4710818264219375E-4	7.108591555460368E-5	1.3540174391353083E-5	7.785600275028022E-5
60-61	0.040034910631633225	2.538782698378703E-4	7.785600275028022E-5	1.3540174391353083E-5	7.785600275028022E-5
62-63	0.04973813810483663	2.657259224303042E-4	7.785600275028022E-5	1.3540174391353083E-5	7.785600275028022E-5
64-65	0.061582405653672734	2.7080348782706164E-4	7.785600275028022E-5	1.3540174391353083E-5	7.785600275028022E-5
66-67	0.07559987119232102	2.7418853142489993E-4	7.785600275028022E-5	1.3540174391353083E-5	7.785600275028022E-5
68-69	0.0922982912604572	2.8265114041949556E-4	7.954852454919936E-5	1.3540174391353083E-5	7.785600275028022E-5
70-71	0.11268133128484034	2.843436622184147E-4	8.124104634811849E-5	1.6925217989191352E-5	7.785600275028022E-5
72-73	0.13837888975782958	2.843436622184147E-4	8.462608994595677E-5	1.6925217989191352E-5	8.462608994595677E-5
74-75	0.1711850397862792	2.9280627121301044E-4	8.462608994595677E-5	1.6925217989191352E-5	8.462608994595677E-5
76-77	0.21203574592499141	3.3342679438706964E-4	8.462608994595677E-5	1.6925217989191352E-5	8.63186117448759E-5
78-79	0.2613439834929026	3.50352012376261E-4	8.462608994595677E-5	1.6925217989191352E-5	8.801113354379504E-5
80-81	0.3199153928662982	3.5204453417518014E-4	8.462608994595677E-5	1.6925217989191352E-5	9.816626433730985E-5
82-83	0.3911197849468262	3.5204453417518014E-4	8.970365534271417E-5	1.6925217989191352E-5	1.0155130793514811E-4
84-85	0.48024459783431006	3.571220995719376E-4	9.308869894055244E-5	1.6925217989191352E-5	1.0832139513082466E-4
86-87	0.5918342525588476	3.774323611589671E-4	9.478122073947157E-5	1.6925217989191352E-5	1.1170643872866293E-4
88-89	0.7267451651506918	4.0451270994167337E-4	9.985878613622898E-5	1.6925217989191352E-5	1.2016904772325861E-4
90	0.8424662655863909	4.163603625341073E-4	1.0155130793514811E-4	1.6925217989191352E-5	1.2186156952217774E-4
>>END_MODULE
