##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654133_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34859877
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.402550531087645	32.0	32.0	32.0	32.0	32.0
2	31.335976572722846	32.0	32.0	32.0	32.0	32.0
3	35.142529877543744	37.0	32.0	37.0	32.0	37.0
4	35.99290307306592	37.0	37.0	37.0	32.0	37.0
5	36.16444492905124	37.0	37.0	37.0	37.0	37.0
6	39.331032550688576	41.0	41.0	41.0	37.0	41.0
7	39.439853617383676	41.0	41.0	41.0	37.0	41.0
8	39.58458863753306	41.0	41.0	41.0	37.0	41.0
9	39.692780700287614	41.0	41.0	41.0	37.0	41.0
10-11	39.777345872448144	41.0	41.0	41.0	37.0	41.0
12-13	39.71044646829936	41.0	41.0	41.0	37.0	41.0
14-15	39.69982678080017	41.0	41.0	41.0	37.0	41.0
16-17	39.651344022240814	41.0	41.0	41.0	37.0	41.0
18-19	39.659233823458415	41.0	41.0	41.0	37.0	41.0
20-21	39.5949138604247	41.0	41.0	41.0	37.0	41.0
22-23	39.517607620933376	41.0	41.0	41.0	37.0	41.0
24-25	39.53239076259506	41.0	41.0	41.0	37.0	41.0
26-27	39.36986960682621	41.0	41.0	41.0	37.0	41.0
28-29	39.43062198699094	41.0	41.0	41.0	37.0	41.0
30-31	39.513348340270966	41.0	41.0	41.0	37.0	41.0
32-33	39.49688930342468	41.0	41.0	41.0	37.0	41.0
34-35	39.31209590900163	41.0	41.0	41.0	37.0	41.0
36-37	39.18923096028136	41.0	41.0	41.0	37.0	41.0
38-39	39.239874082171895	41.0	41.0	41.0	37.0	41.0
40-41	39.21227823035635	41.0	41.0	41.0	37.0	41.0
42-43	39.08482481163087	41.0	41.0	41.0	37.0	41.0
44-45	39.1032304847203	41.0	41.0	41.0	37.0	41.0
46-47	39.08652838907034	41.0	41.0	41.0	37.0	41.0
48-49	39.11942385797862	41.0	41.0	41.0	37.0	41.0
50-51	39.026802561581036	41.0	41.0	41.0	37.0	41.0
52-53	39.10767903742173	41.0	41.0	41.0	37.0	41.0
54-55	38.97901689383471	41.0	41.0	41.0	37.0	41.0
56-57	38.96929583256992	41.0	41.0	41.0	37.0	41.0
58-59	38.959356540472015	41.0	41.0	41.0	37.0	41.0
60-61	38.97240057674328	41.0	41.0	41.0	37.0	41.0
62-63	38.98693235492483	41.0	41.0	41.0	37.0	41.0
64-65	38.98357814056544	41.0	41.0	41.0	37.0	41.0
66-67	38.92789237322897	41.0	41.0	41.0	34.5	41.0
68-69	38.883092258185535	41.0	41.0	41.0	32.0	41.0
70-71	38.83737416801557	41.0	41.0	41.0	32.0	41.0
72-73	38.794977460763846	41.0	41.0	41.0	32.0	41.0
74-75	38.77585223550846	41.0	41.0	41.0	32.0	41.0
76-77	37.97014219241221	41.0	39.0	41.0	32.0	41.0
78-79	38.51611596334663	41.0	39.0	41.0	32.0	41.0
80-81	38.91492480022233	41.0	41.0	41.0	34.5	41.0
82-83	38.950880506549126	41.0	41.0	41.0	37.0	41.0
84-85	38.830351179953965	41.0	41.0	41.0	32.0	41.0
86-87	38.872907282489834	41.0	41.0	41.0	32.0	41.0
88-89	38.757568952409095	41.0	41.0	41.0	32.0	41.0
90-91	38.667279950528794	41.0	41.0	41.0	32.0	41.0
92-93	38.64996283549709	41.0	41.0	41.0	32.0	41.0
94-95	38.48004363297094	41.0	41.0	41.0	32.0	41.0
96-97	38.44790104967955	41.0	39.0	41.0	32.0	41.0
98-99	38.37415015836115	41.0	37.0	41.0	32.0	41.0
100-101	37.28609368013548	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	39.0
13	113.0
14	552.0
15	2447.0
16	10076.0
17	25713.0
18	41817.0
19	53047.0
20	61546.0
21	71784.0
22	83357.0
23	98393.0
24	114067.0
25	131577.0
26	152296.0
27	175978.0
28	203076.0
29	235900.0
30	277315.0
31	329141.0
32	388243.0
33	466170.0
34	578948.0
35	741963.0
36	970706.0
37	1350847.0
38	2242296.0
39	5576913.0
40	2.0475557E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.07074613343539	22.542730806889946	10.195276897120449	30.19124616255422
2	30.14121720770046	25.308260883909274	28.406171959383382	16.14434994900689
3	21.368198301462776	27.37987570543966	29.229566328791158	22.022359664306407
4	25.00944497185843	31.605994041621216	22.95949737890198	20.425063607618377
5	27.327306408288944	33.21314236935613	21.113340709263923	18.346210513091005
6	22.961596433022176	36.83594863628125	21.261244555759188	18.941210374937384
7	21.997705151663986	21.845292842746318	35.9373151880983	20.219686817491393
8	23.265601198377126	24.768776806534614	26.294889330885546	25.670732664202717
9	23.61818052151895	23.320326733797465	29.071580208036362	23.98991253664722
10-11	25.426026810606416	29.815041780907826	23.07863565159546	21.6802957568903
12-13	25.683932534238778	24.872438713888762	26.16465094792711	23.27897780394535
14-15	24.985597013215806	26.39598734072028	26.263756568343645	22.35465907772027
16-17	25.7993903874214	26.169009070464888	25.57416133089519	22.457439211218524
18-19	25.397016185680076	26.757161406696838	25.603584569691733	22.242237837931352
20-21	25.513910399217433	26.598580335768013	25.562512413896666	22.324996851117884
22-23	25.396074205907563	26.430429878135325	25.66680601290069	22.50668990305642
24-25	25.199797358772773	26.381419321166234	25.95865622058676	22.460127099474228
26-27	25.342993713278545	26.551897727367923	25.720141917432255	22.384966641921277
28-29	25.35981286090696	26.384434681838155	25.739695293652055	22.516057163602834
30-31	25.08482048668512	26.29773623258641	26.02770355008953	22.58973973063894
32-33	25.199694353873305	26.509148549247367	25.80774774862389	22.483409348255435
34-35	25.341543648987518	26.597804048381928	25.492058165706936	22.568594136923615
36-37	25.08128150511819	26.389174423534477	26.117342412656093	22.412201658691234
38-39	25.334861616103804	26.576372610659565	25.803156777512353	22.285608995724278
40-41	25.36067642378826	26.39011613482482	25.668391766433157	22.580815674953772
42-43	24.96521853815122	26.411283294716277	26.12621344934068	22.49728471779183
44-45	25.150025880034494	26.431576728980527	26.09888588760945	22.319511503375537
46-47	25.460135406387558	26.199145363434834	25.744809145453512	22.595910084724093
48-49	25.023935417578507	26.314761040587904	26.167549472267503	22.493754069566084
50-51	25.33548956218037	26.304148647647622	25.850661514531147	22.509700275640856
52-53	25.50025269485838	26.267784369789148	25.71317391469556	22.518789020656918
54-55	24.899143379657517	26.363902913587882	26.196533669151496	22.540420037603106
56-57	25.00793419134108	26.439037954267956	25.97032821874693	22.582699635644033
58-59	25.3862019973313	26.20046395691634	25.748389616229673	22.66494442952269
60-61	25.161100710884927	26.325846676545027	26.029455649459997	22.48359696311005
62-63	25.089620593958962	26.252258266827372	26.22374177563393	22.43437936357974
64-65	25.271723274211883	26.19573014848726	26.018974829188785	22.513571748112074
66-67	25.087808188585953	26.28996308400498	26.212321600697486	22.40990712671158
68-69	25.100491008844173	26.530950415904904	25.981354930553596	22.387203644697326
70-71	25.481279043696336	26.295061599635282	25.74560073351912	22.47805862314926
72-73	24.978452446750346	26.31388292991414	26.296771975133478	22.41089264820204
74-75	24.92595432452437	26.49046988414706	26.136700046232956	22.446875745095614
76-77	25.500871782661676	26.224339579790616	25.79704106695704	22.47774757059067
78-79	25.00463164397404	26.453176055447123	26.038469090004	22.50372321057484
80-81	25.170943690525995	26.438016652442574	26.009419254592192	22.38162040243924
82-83	25.428354138477022	26.20642677411011	25.910424091973542	22.454794995439332
84-85	25.180693133968624	26.418505423702587	26.15475166969423	22.246049772634557
86-87	25.136326125014964	26.657734530082816	25.86420393671417	22.34173540818805
88-89	25.39327177553493	26.289089881441313	25.915514005971787	22.40212433705197
90-91	24.972951776900686	26.51938764736349	26.208864335516648	22.298796240219172
92-93	25.148607201255142	26.61347891816436	25.96038386435299	22.2775300162275
94-95	25.577241144246393	26.44750684106121	25.755879113966113	22.219372900726285
96-97	25.367377406678564	26.343722738787246	26.20556077174364	22.08333908279055
98-99	25.453673360688594	26.67021951413233	25.77269089049365	22.103416234685426
100-101	25.68942081790249	26.42413953570637	25.72109667788911	22.165342968502028
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	40.0
2	22.0
3	27.0
4	30.0
5	42.5
6	56.5
7	68.5
8	87.0
9	106.0
10	132.0
11	166.0
12	193.5
13	247.0
14	319.0
15	362.5
16	437.0
17	579.0
18	714.5
19	898.0
20	1229.5
21	1583.5
22	2066.0
23	2749.0
24	3627.0
25	4949.5
26	6774.0
27	9289.5
28	13139.5
29	18394.0
30	25707.0
31	36493.0
32	52342.0
33	75364.0
34	111092.0
35	166250.0
36	247969.5
37	366047.0
38	539283.5
39	773640.5
40	1042358.5
41	1319037.0
42	1598331.0
43	1848081.5
44	2061051.0
45	2273795.5
46	2360281.5
47	2331399.0
48	2252636.0
49	2107911.5
50	1931051.0
51	1771412.5
52	1586050.5
53	1347359.0
54	1141161.5
55	965435.5
56	809200.0
57	665935.5
58	538454.0
59	423967.5
60	353444.5
61	315981.0
62	277081.0
63	228229.0
64	178968.5
65	144810.0
66	123340.0
67	104278.5
68	78807.0
69	56399.5
70	42498.0
71	34398.5
72	26858.5
73	19127.0
74	13274.0
75	8439.0
76	5198.0
77	3152.5
78	1822.5
79	1111.5
80	742.0
81	534.0
82	407.5
83	310.5
84	247.5
85	203.0
86	168.5
87	156.5
88	147.5
89	126.5
90	100.0
91	86.5
92	77.5
93	66.5
94	60.0
95	60.5
96	64.0
97	70.0
98	89.0
99	180.0
100	713.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007765374502038547
2	0.0063884333269449
3	0.0016867529394897175
4	1.692490194385941E-4
5	0.0145324666521342
6	0.0016150372532869235
7	0.005163529406601176
8	0.009764807833372448
9	0.0033362137221539826
10-11	0.0066810333266523
12-13	0.004683034309042456
14-15	0.00972894999027105
16-17	9.179607833957647E-5
18-19	0.013171302928005169
20-21	0.006507481366041538
22-23	0.008767959795153608
24-25	0.012008074497795847
26-27	0.010192233323141101
28-29	0.006833070581402223
30-31	0.011219201949565112
32-33	0.005503461759202421
34-35	0.005098985289018662
36-37	0.009291484304434007
38-39	0.010791776459796459
40-41	0.009033307834103946
42-43	0.012576062732521976
44-45	0.008293201952491112
46-47	0.004449241172021347
48-49	0.010272554891688228
50-51	0.010473358813056053
52-53	0.007511500972880656
54-55	0.01064834508739087
56-57	0.012630566654036099
58-59	0.006065712739032327
60-61	0.0024655852916520616
62-63	0.0132157666534509
64-65	0.005172135288945512
66-67	0.0063755245034283975
68-69	0.01582048037633638
70-71	0.006203406856541692
72-73	0.0059982999940017
74-75	0.01397164998602835
76-77	0.0065361676405226565
78-79	0.01303074018304769
80-81	1.7211764688670588E-5
82-83	0.0074340480317816385
84-85	0.01711279704171073
86-87	0.0020926637233975322
88-89	0.0104360666562306
90-91	0.0037363872511655735
92-93	0.012137162732960876
94-95	0.010400208813129202
96-97	0.004343101956441212
98-99	0.009548226461040008
100-101	0.01055798332277535
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4859877E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.380659738567182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.258518251591624	16.42420785822613
2	14.638863847903307	7.723657722614989
3	6.632010159101709	5.248704101699488
4	3.8797291253799737	4.093992557378317
5	2.5205811395275703	3.3247296692663375
6	1.795193074533354	2.8415026598578
7	1.2789580175035054	2.361782939577069
8	0.9429311893379424	1.9900117490245377
9	0.7382979139477437	1.7529107448194443
>10	4.6183169727949585	22.829126610009606
>50	0.39202623993391494	7.15499154621159
>100	0.2678517672299518	13.773758151996207
>500	0.025112935835028858	4.531154211094139
>1k	0.011301177631528	5.251663778803187
>5k	2.6253030399179774E-4	0.4486814343882462
>10k+	4.5657444172486565E-5	0.2491242650330041
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGAAAATCTATCATTTCCAGTCCTGGTTAGCCAACATGACACTTTCAATT	42588	0.12216910576018385	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.597843130657059E-5	0.0	8.605882344335294E-6	2.8686274481117647E-6	0.0
2	6.884705875468235E-5	1.1474509792447059E-5	1.7211764688670588E-5	2.8686274481117647E-6	5.7372548962235294E-6
3	1.1474509792447059E-4	1.4343137240558824E-5	1.7211764688670588E-5	2.8686274481117647E-6	5.7372548962235294E-6
4	1.9219803902348822E-4	1.4343137240558824E-5	3.155490192922941E-5	2.8686274481117647E-6	5.7372548962235294E-6
5	2.352274507451647E-4	1.7211764688670588E-5	3.155490192922941E-5	2.8686274481117647E-6	5.7372548962235294E-6
6	2.753882350187294E-4	2.0080392136782353E-5	4.302941172167647E-5	2.8686274481117647E-6	5.7372548962235294E-6
7	3.012058820517353E-4	2.0080392136782353E-5	4.87666666179E-5	2.8686274481117647E-6	5.7372548962235294E-6
8	3.471039212215235E-4	2.0080392136782353E-5	6.024117641034706E-5	1.1474509792447059E-5	5.7372548962235294E-6
9	3.7005294080641763E-4	4.0160784273564706E-5	6.597843130657059E-5	1.1474509792447059E-5	5.7372548962235294E-6
10-11	4.618490191459941E-4	4.302941172167647E-5	7.888725482307354E-5	1.1474509792447059E-5	1.0040196068391176E-5
12-13	5.335647053487882E-4	5.020098034195588E-5	9.609901951174412E-5	1.1474509792447059E-5	1.4343137240558824E-5
14-15	6.440068621010912E-4	5.1635294066011765E-5	1.1904803909663823E-4	1.7211764688670588E-5	1.4343137240558824E-5
16-17	7.54449018853394E-4	5.1635294066011765E-5	1.764205880588735E-4	1.7211764688670588E-5	1.4343137240558824E-5
18-19	9.251323520160441E-4	5.1635294066011765E-5	2.0654117626404706E-4	2.7251960757061765E-5	1.4343137240558824E-5
20-21	0.0011273705871079234	5.450392151412353E-5	2.266215684008294E-4	3.012058820517353E-5	1.4343137240558824E-5
22-23	0.0014185362730912677	6.167549013440295E-5	2.8399411736306467E-4	3.155490192922941E-5	1.4343137240558824E-5
24-25	0.0018531333314802	7.745294109901765E-5	2.8829705853523235E-4	3.155490192922941E-5	1.4343137240558824E-5
26-27	0.002452676468135559	8.462450971929707E-5	2.897313722592882E-4	3.155490192922941E-5	1.4343137240558824E-5
28-29	0.0030837745067201472	8.89274508914647E-5	3.1268039184418234E-4	3.155490192922941E-5	1.7211764688670588E-5
30-31	0.0039673117607385705	1.0327058813202353E-4	3.155490192922941E-4	3.155490192922941E-5	2.0080392136782353E-5
32-33	0.0049469480342687385	1.0900784302824706E-4	3.184176467404059E-4	3.155490192922941E-5	3.155490192922941E-5
34-35	0.006048500974343656	1.1187647047635882E-4	3.5140686239369114E-4	3.155490192922941E-5	3.729215682545294E-5
36-37	0.007469905874883035	1.2478529399286177E-4	3.958705878394235E-4	3.155490192922941E-5	4.0160784273564706E-5
38-39	0.009291484304434005	1.3195686261314118E-4	3.973049015634794E-4	3.155490192922941E-5	4.0160784273564706E-5
40-41	0.011529013713961183	1.448656861296441E-4	4.044764701837588E-4	3.155490192922941E-5	4.0160784273564706E-5
42-43	0.014258512730839527	1.477343135777559E-4	4.432029407332676E-4	3.155490192922941E-5	4.0160784273564706E-5
44-45	0.01725335978666821	1.5060294102586764E-4	4.503745093535471E-4	3.155490192922941E-5	4.302941172167647E-5
46-47	0.020694278410678273	1.5634019592209117E-4	5.020098034195588E-4	3.155490192922941E-5	4.302941172167647E-5
48-49	0.0249685333083648	1.6064313709425882E-4	5.220901955563412E-4	3.155490192922941E-5	4.5898039169788235E-5
50-51	0.030335735263781912	1.8502647040320882E-4	5.24958823004453E-4	3.155490192922941E-5	4.733235289384412E-5
52-53	0.03657643427714906	1.9219803902348822E-4	5.321303916247323E-4	3.155490192922941E-5	4.87666666179E-5
54-55	0.04341237348599939	2.022382350918794E-4	5.507764700374588E-4	3.155490192922941E-5	4.87666666179E-5
56-57	0.05108738622342242	2.0367254881593528E-4	5.866343131388559E-4	3.155490192922941E-5	4.87666666179E-5
58-59	0.06021105582214189	2.094098037121588E-4	6.024117641034706E-4	3.155490192922941E-5	4.87666666179E-5
60-61	0.07131407836005847	2.122784311602706E-4	6.440068621010912E-4	3.155490192922941E-5	4.87666666179E-5
62-63	0.08532158618918823	2.1514705860838234E-4	7.114196071317175E-4	3.155490192922941E-5	5.1635294066011765E-5
64-65	0.10163547048659982	2.1658137233243824E-4	7.415401953368911E-4	3.155490192922941E-5	5.593823523817941E-5
66-67	0.1205382910559323	2.2518725467677352E-4	7.472774502331146E-4	3.2989215653285294E-5	5.7372548962235294E-5
68-69	0.14239149495564773	2.423990193654441E-4	7.573176463015059E-4	3.4423529377341177E-5	6.024117641034706E-5
70-71	0.16849743904718884	2.4957058798572354E-4	7.601862737496176E-4	3.4423529377341177E-5	6.310980385845882E-5
72-73	0.2009215350931961	2.4957058798572354E-4	7.860039207826235E-4	3.4423529377341177E-5	6.310980385845882E-5
74-75	0.24131898113123004	2.4957058798572354E-4	7.874382345066794E-4	3.4423529377341177E-5	6.310980385845882E-5
76-77	0.28949901343599116	2.524392154338353E-4	7.931754894029029E-4	3.4423529377341177E-5	6.310980385845882E-5
78-79	0.3473821780839904	2.667823526743941E-4	7.946098031269589E-4	3.4423529377341177E-5	6.45441175825147E-5
80-81	0.4157257927215291	2.710852938465618E-4	8.003470580231824E-4	3.4423529377341177E-5	6.597843130657059E-5
82-83	0.4981730142077093	2.7251960757061766E-4	8.0464999919535E-4	3.4423529377341177E-5	6.597843130657059E-5
84-85	0.6006575984189502	2.7395392129467354E-4	8.118215678156294E-4	3.4423529377341177E-5	6.741274503062648E-5
86-87	0.7269345786848301	2.768225487427853E-4	8.118215678156294E-4	3.4423529377341177E-5	6.884705875468235E-5
88-89	0.8755309148107436	2.940343134314559E-4	8.161245089877971E-4	3.4423529377341177E-5	7.171568620279412E-5
90	1.0016759382140104	3.155490192922941E-4	8.232960776080765E-4	3.4423529377341177E-5	7.745294109901765E-5
>>END_MODULE
