##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654114_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34533747
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.331796952123383	32.0	32.0	32.0	32.0	32.0
2	31.460476009162864	32.0	32.0	32.0	32.0	32.0
3	34.00536391258093	37.0	32.0	37.0	32.0	37.0
4	35.49719333960488	37.0	37.0	37.0	32.0	37.0
5	35.570313930892006	37.0	37.0	37.0	32.0	37.0
6	39.094339284989836	41.0	41.0	41.0	37.0	41.0
7	39.129591932204754	41.0	41.0	41.0	37.0	41.0
8	39.32815231431446	41.0	41.0	41.0	37.0	41.0
9	39.314966140222204	41.0	41.0	41.0	37.0	41.0
10-11	39.286262536179464	41.0	41.0	41.0	37.0	41.0
12-13	39.498133897836226	41.0	41.0	41.0	37.0	41.0
14-15	39.44044661588561	41.0	41.0	41.0	37.0	41.0
16-17	39.306787908071485	41.0	41.0	41.0	37.0	41.0
18-19	39.42924267673589	41.0	41.0	41.0	37.0	41.0
20-21	39.47544024110677	41.0	41.0	41.0	37.0	41.0
22-23	39.17824285039211	41.0	41.0	41.0	37.0	41.0
24-25	39.21822772373933	41.0	41.0	41.0	37.0	41.0
26-27	39.25602195730455	41.0	41.0	41.0	37.0	41.0
28-29	39.05791830234929	41.0	41.0	41.0	37.0	41.0
30-31	39.15452270499347	41.0	41.0	41.0	37.0	41.0
32-33	39.34880144920272	41.0	41.0	41.0	37.0	41.0
34-35	39.295635194176874	41.0	41.0	41.0	37.0	41.0
36-37	39.059166197632706	41.0	41.0	41.0	37.0	41.0
38-39	38.97356888321444	41.0	41.0	41.0	37.0	41.0
40-41	38.90867121948858	41.0	41.0	41.0	37.0	41.0
42-43	38.90544858917279	41.0	41.0	41.0	37.0	41.0
44-45	38.926186390952594	41.0	41.0	41.0	37.0	41.0
46-47	38.88851641265571	41.0	41.0	41.0	34.5	41.0
48-49	38.85236442775816	41.0	41.0	41.0	32.0	41.0
50-51	38.91388328060665	41.0	41.0	41.0	32.0	41.0
52-53	38.92440978096006	41.0	41.0	41.0	34.5	41.0
54-55	38.91935278555206	41.0	41.0	41.0	32.0	41.0
56-57	38.889341735780945	41.0	41.0	41.0	32.0	41.0
58-59	38.80029579471929	41.0	41.0	41.0	32.0	41.0
60-61	38.842994766829094	41.0	41.0	41.0	32.0	41.0
62-63	38.842241112729525	41.0	41.0	41.0	32.0	41.0
64-65	38.822532840123024	41.0	41.0	41.0	32.0	41.0
66-67	38.78691905341172	41.0	41.0	41.0	32.0	41.0
68-69	38.771630587900006	41.0	41.0	41.0	32.0	41.0
70-71	38.751715792670865	41.0	41.0	41.0	32.0	41.0
72-73	38.72819912939074	41.0	41.0	41.0	32.0	41.0
74-75	38.70882398020696	41.0	41.0	41.0	32.0	41.0
76-77	37.820578476468256	41.0	39.0	41.0	29.5	41.0
78-79	38.29364642938978	41.0	39.0	41.0	32.0	41.0
80-81	38.64688465459598	41.0	41.0	41.0	32.0	41.0
82-83	38.821182320586296	41.0	41.0	41.0	32.0	41.0
84-85	38.77873572479697	41.0	41.0	41.0	32.0	41.0
86-87	38.715960593560844	41.0	41.0	41.0	32.0	41.0
88-89	38.65986995271611	41.0	41.0	41.0	32.0	41.0
90-91	38.60701262738735	41.0	41.0	41.0	32.0	41.0
92-93	38.534868935595085	41.0	39.0	41.0	32.0	41.0
94-95	38.52266345438855	41.0	39.0	41.0	32.0	41.0
96-97	38.418567944567386	41.0	37.0	41.0	32.0	41.0
98-99	38.209309099878446	41.0	37.0	41.0	32.0	41.0
100-101	37.20189273118842	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	29.0
13	123.0
14	565.0
15	2318.0
16	9192.0
17	22934.0
18	38259.0
19	51337.0
20	62821.0
21	75483.0
22	89170.0
23	104987.0
24	121980.0
25	141072.0
26	163506.0
27	188444.0
28	217672.0
29	254517.0
30	298640.0
31	352469.0
32	418276.0
33	502509.0
34	619602.0
35	783149.0
36	1039713.0
37	1493418.0
38	2599515.0
39	6982462.0
40	1.7899585E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.31300952790273	21.038953860625952	11.506665198122775	34.141371413348544
2	29.46659683352635	25.276090080812835	28.824908574212927	16.43240451144789
3	21.418946036996946	27.5933509509273	28.551088995490197	22.43661401658556
4	24.79634486231685	30.77011596801239	23.276107281378994	21.157431888291764
5	27.55710864930558	32.11932234442312	21.859150986185313	18.46441802008599
6	22.699254152756723	36.81865741357287	21.680404388206124	18.80168404546428
7	22.126353679489224	22.107488075360024	35.45855304957206	20.30760519557869
8	23.21115921767771	24.77776014285389	26.78333167843038	25.227748961038028
9	23.58978016489204	23.16746282990954	29.55252727136734	23.690229733831085
10-11	25.083156505947613	29.90797273316789	23.424501728428694	21.584369032455797
12-13	25.546667741561418	24.930986443107088	26.152321129093544	23.370024686237947
14-15	24.82159986867339	26.5786767940357	26.159573706264773	22.440149631026138
16-17	25.595096384507897	26.14351075690499	25.574132607623817	22.687260250963295
18-19	25.229502371579283	26.658281919603322	25.660069707482297	22.452146001335098
20-21	25.4482688972697	26.569505270462063	25.535642628610606	22.44658320365763
22-23	25.334422648583537	26.394699530640008	25.627070789941197	22.643807030835255
24-25	24.944197115355518	26.386859613375872	25.99628358188944	22.672659689379167
26-27	25.171787758797215	26.565392686753626	25.85288239935273	22.409937155096436
28-29	25.20999815784803	26.277464631891114	25.772531398715167	22.740005811545696
30-31	24.96408513098796	26.286118039840854	26.001396546977656	22.748400282193533
32-33	25.120947159768665	26.495839359786878	25.796914817768744	22.586298662675716
34-35	25.17511493901893	26.468559869857156	25.633688113832537	22.72263707729138
36-37	24.874685622733033	26.390617270694662	26.124781651988126	22.60991545458418
38-39	25.217110375456304	26.558381402274208	25.839489807901895	22.3850184143676
40-41	25.308394713148274	26.302804616018065	25.656468728979803	22.73233194185386
42-43	24.7760055440749	26.382959371211285	26.17805655294618	22.66297853176764
44-45	25.032716477604268	26.492236604465653	26.01642098233511	22.458625935594974
46-47	25.320099468418366	26.24169885117996	25.77466095497975	22.66354072542192
48-49	24.8907029386004	26.303264413514455	26.175335694523316	22.630696953361834
50-51	25.18871467958574	26.32426188794399	25.864459480750813	22.62256395171946
52-53	25.379922389379672	26.210954880519576	25.694837450186625	22.714285279914122
54-55	24.760899763611256	26.2698657537157	26.227680728684337	22.741553753988708
56-57	24.935332440794493	26.44069212615247	25.96097120175869	22.663004231294348
58-59	25.25602420148616	26.139484661192427	25.771729896555968	22.832761240765446
60-61	25.015199991903568	26.28657852551167	26.025849419041275	22.672372063543488
62-63	25.03687853740722	26.267153002974474	26.109200896271425	22.586767563346882
64-65	25.19820539601451	26.173174894690693	25.918631129138692	22.7099885801561
66-67	24.940703312467203	26.29524431552847	26.212007787179164	22.552044584825165
68-69	25.104889854106467	26.522969349827836	25.891834872621327	22.480305923444373
70-71	25.37639729091452	26.26461907092542	25.696119775886437	22.66286386227362
72-73	24.85623555416677	26.39346376169374	26.145638062385757	22.60466262175373
74-75	24.915470520973557	26.539902913570202	25.97483542326274	22.569791142193505
76-77	25.447238566826307	26.195282111889313	25.671066530175146	22.686412791109234
78-79	24.903760081406748	26.47561673513158	25.951765384740906	22.668857798720772
80-81	25.181849656895857	26.473098922946853	25.85550016662626	22.489551253531033
82-83	25.402979340163533	26.1544717264654	25.786665699097718	22.655883234273347
84-85	25.088249790565065	26.411084834805536	26.071868220711835	22.42879715391756
86-87	25.18360663266572	26.683348320122924	25.68563295491798	22.447412092293373
88-89	25.369287760431014	26.30904748841495	25.7254386018128	22.596226149341238
90-91	24.949665884677692	26.52568278525332	26.062793381602077	22.461857948466914
92-93	25.23648183473354	26.621915596755336	25.736058696415448	22.405543872095677
94-95	25.549399548215835	26.382077797697423	25.6257205451815	22.44280210890524
96-97	25.272523633063493	26.376105294882006	26.019830786114323	22.33154028594018
98-99	25.437903233337657	26.66988236215638	25.609503516741633	22.28271088776434
100-101	25.659496626176008	26.369231875404942	25.562715073234592	22.408556425184454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	35.0
1	30.0
2	27.0
3	27.5
4	37.0
5	43.5
6	51.0
7	70.5
8	88.5
9	105.5
10	139.0
11	178.0
12	197.5
13	226.0
14	301.5
15	395.0
16	461.0
17	554.5
18	715.5
19	886.0
20	1155.0
21	1513.0
22	1880.0
23	2391.5
24	3180.5
25	4421.5
26	6055.5
27	8077.5
28	11224.0
29	16061.0
30	22921.0
31	32977.5
32	48488.0
33	72870.5
34	112134.0
35	173500.0
36	264181.0
37	394171.0
38	574898.0
39	808344.0
40	1071908.5
41	1337287.5
42	1606028.5
43	1839737.5
44	2028871.0
45	2197248.5
46	2250951.0
47	2218584.0
48	2157304.5
49	2043552.0
50	1879684.5
51	1713216.0
52	1530018.5
53	1318544.5
54	1137264.5
55	978528.5
56	828391.5
57	683346.5
58	554573.5
59	437811.0
60	365879.5
61	327244.0
62	290938.0
63	244895.0
64	191705.5
65	156342.0
66	135437.0
67	115641.5
68	88905.5
69	64641.0
70	48751.5
71	38755.0
72	29576.0
73	20364.0
74	13259.5
75	7922.0
76	4694.0
77	2807.0
78	1725.0
79	1171.5
80	797.0
81	597.5
82	468.0
83	379.0
84	303.0
85	241.5
86	215.0
87	188.5
88	159.5
89	142.5
90	121.0
91	99.5
92	85.0
93	69.5
94	58.5
95	50.5
96	55.5
97	58.0
98	63.0
99	107.5
100	528.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04138850035589824
2	0.0
3	2.519274841505036E-4
4	0.0
5	0.008145655320866281
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.9980455639522697E-4
12-13	3.865783808516348E-4
14-15	0.0
16-17	0.00107286359629611
18-19	2.3165745669011823E-5
20-21	0.0014029754720795286
22-23	3.648604942869362E-4
24-25	0.002994172627719778
26-27	0.0
28-29	0.0022166722887035687
30-31	0.0
32-33	0.004695407075287834
34-35	0.0
36-37	0.0
38-39	0.0022977523985451102
40-41	0.0
42-43	8.774026172138227E-4
44-45	8.904333491526418E-4
46-47	0.0016650379699602246
48-49	0.0013132082076121078
50-51	0.0
52-53	0.0031910814659063783
54-55	4.792413635276821E-4
56-57	0.003386542444988666
58-59	0.0
60-61	3.330075939920449E-5
62-63	0.0032649222802263534
64-65	0.0
66-67	0.0024077896904729163
68-69	4.0540054920770686E-4
70-71	0.00285662601281002
72-73	0.0
74-75	2.4468818862893735E-4
76-77	0.003470518273038834
78-79	0.0
80-81	0.003981612536861407
82-83	0.001340717530594059
84-85	0.002737901566256335
86-87	0.0
88-89	0.001000470641080448
90-91	3.474861850351773E-5
92-93	3.532776214524303E-4
94-95	0.0
96-97	0.002387519663012531
98-99	1.3030731938819149E-5
100-101	8.875376309440154E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4533747E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.08530496381225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.68360545814691	16.090356596432358
2	14.555449338252648	7.593666697472792
3	6.861596233701473	5.369604908839454
4	4.04848950610909	4.224243336385969
5	2.5174620828274676	3.2834383082694236
6	1.7330435881285917	2.7124182307148255
7	1.3007126697444886	2.375064066240558
8	0.9809640565222938	2.0470997258337915
9	0.7716228706790096	1.8115216108842807
>10	4.809207756767774	23.559620024104834
>50	0.42355583406534825	7.682759218240734
>100	0.28004700972871954	14.18843092378181
>500	0.024088236690714512	4.271542834259104
>1k	0.009969991246877473	4.383379874984507
>5k	1.3902554140309808E-4	0.22196742391414762
>10k+	4.634184713436602E-5	0.18488621964137517
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.5018645963903076E-5	2.895718208626478E-6	1.4478591043132389E-5	0.0	0.0
2	6.081008238115603E-5	1.1582872834505912E-5	3.1852900294891256E-5	0.0	0.0
3	9.266298267604729E-5	1.1582872834505912E-5	3.1852900294891256E-5	0.0	0.0
4	1.6216021968308277E-4	1.1582872834505912E-5	4.054005492077069E-5	0.0	0.0
5	1.7663881072621515E-4	1.4478591043132389E-5	4.922720954665012E-5	0.0	0.0
6	2.200745838556123E-4	1.4478591043132389E-5	6.081008238115603E-5	0.0	0.0
7	2.3744889310737117E-4	1.7374309251758865E-5	7.239295521566195E-5	0.0	0.0
8	2.6640607519363594E-4	1.7374309251758865E-5	9.845441909330024E-5	0.0	0.0
9	2.9246753907127425E-4	2.0270027460385346E-5	1.0135013730192672E-4	0.0	0.0
10-11	3.431426077222376E-4	2.7509322981951538E-5	1.1872444655368559E-4	0.0	2.895718208626478E-6
12-13	4.169834220422128E-4	3.1852900294891256E-5	1.5492092416151656E-4	0.0	2.895718208626478E-6
14-15	5.038549683010071E-4	3.474861850351773E-5	1.8098238803915487E-4	1.447859104313239E-6	2.895718208626478E-6
16-17	5.907265145598014E-4	3.474861850351773E-5	2.4468818862893735E-4	2.895718208626478E-6	2.895718208626478E-6
18-19	6.877330745487884E-4	3.474861850351773E-5	2.982589754885272E-4	2.895718208626478E-6	2.895718208626478E-6
20-21	7.977703664765947E-4	5.21229277552766E-5	3.431426077222376E-4	2.895718208626478E-6	2.895718208626478E-6
22-23	9.251819676561596E-4	5.646650506821632E-5	4.24222717563779E-4	2.895718208626478E-6	2.895718208626478E-6
24-25	0.0011814530291196028	7.09450961113487E-5	4.4883632233710404E-4	2.895718208626478E-6	2.895718208626478E-6
26-27	0.0014840555819210697	8.687154625879433E-5	4.5897133606729673E-4	2.895718208626478E-6	2.895718208626478E-6
28-29	0.0018271981896433075	9.266298267604728E-5	4.966156727794409E-4	2.895718208626478E-6	2.895718208626478E-6
30-31	0.0023744889310737117	1.3175517849250474E-4	5.139899820311998E-4	2.895718208626478E-6	2.895718208626478E-6
32-33	0.0030100990778672235	1.5781664237014305E-4	5.226771366570792E-4	2.895718208626478E-6	2.895718208626478E-6
34-35	0.003762985812110108	1.6216021968308277E-4	5.965179509770544E-4	2.895718208626478E-6	4.343577312939717E-6
36-37	0.0046678977523058815	1.708473743089622E-4	6.298187103762589E-4	2.895718208626478E-6	5.791436417252956E-6
38-39	0.005892786554554882	1.824302471434681E-4	6.674630470884031E-4	2.895718208626478E-6	5.791436417252956E-6
40-41	0.00746081796452612	1.983566972909137E-4	7.051073838005473E-4	2.895718208626478E-6	5.791436417252956E-6
42-43	0.009500851442503472	2.0559599281247992E-4	7.731567617032696E-4	2.895718208626478E-6	5.791436417252956E-6
44-45	0.011933254737749715	2.1428314743835935E-4	7.876353527464019E-4	2.895718208626478E-6	5.791436417252956E-6
46-47	0.01456835830759981	2.229703020642388E-4	8.48445435127558E-4	2.895718208626478E-6	5.791436417252956E-6
48-49	0.017938974302441027	2.461360477332506E-4	8.802983354224492E-4	2.895718208626478E-6	5.791436417252956E-6
50-51	0.022152244295992555	2.7654108892382864E-4	8.947769264655817E-4	2.895718208626478E-6	5.791436417252956E-6
52-53	0.0273848070989806	3.011546936971537E-4	9.497955724294847E-4	2.895718208626478E-6	7.239295521566194E-6
54-55	0.03352952113768599	3.0694613011440666E-4	9.888877682459423E-4	2.895718208626478E-6	8.687154625879432E-6
56-57	0.040580594975691456	3.0839398921871986E-4	0.0010279799640623996	2.895718208626478E-6	8.687154625879432E-6
58-59	0.04885945333415456	3.1418542563597284E-4	0.0010525935688357247	2.895718208626478E-6	8.687154625879432E-6
60-61	0.05892641768644451	3.199768620532258E-4	0.0011162993694255072	2.895718208626478E-6	8.687154625879432E-6
62-63	0.07138524527905993	3.2866401667910525E-4	0.0012089623521015546	2.895718208626478E-6	8.687154625879432E-6
64-65	0.08677453969880534	3.402468895136111E-4	0.0012987296165689753	2.895718208626478E-6	8.687154625879432E-6
66-67	0.10497123292181412	3.590690578696833E-4	0.001308864630299168	5.791436417252956E-6	8.687154625879432E-6
68-69	0.12662686154502725	3.7644336712144215E-4	0.0013262389395509268	5.791436417252956E-6	8.687154625879432E-6
70-71	0.15203534096662027	4.0540054920770686E-4	0.0013305825168638667	5.791436417252956E-6	1.0135013730192673E-5
72-73	0.1835697122585626	4.1553556293789955E-4	0.0013609875580544444	5.791436417252956E-6	1.4478591043132389E-5
74-75	0.2233554325859861	4.1987914025083927E-4	0.001363883276263071	5.791436417252956E-6	1.4478591043132389E-5
76-77	0.2723741504216151	4.2711843577240545E-4	0.0013740182899932636	5.791436417252956E-6	1.4478591043132389E-5
78-79	0.33077644311229826	4.4738846323279084E-4	0.0013899447401407093	5.791436417252956E-6	1.8822168356072105E-5
80-81	0.4002403793599345	4.633149133802364E-4	0.001405871190288155	5.791436417252956E-6	2.3165745669011823E-5
82-83	0.48499225989001427	4.763456453190556E-4	0.0014406198087916727	5.791436417252956E-6	2.3165745669011823E-5
84-85	0.5896217980632105	4.835849408406218E-4	0.0014493069634175521	5.791436417252956E-6	2.3165745669011823E-5
86-87	0.7173432410910985	4.8648065904924824E-4	0.0014594419771477448	5.791436417252956E-6	2.3165745669011823E-5
88-89	0.8700952723143538	4.922720954665012E-4	0.0014681291317736242	5.791436417252956E-6	2.3165745669011823E-5
90	1.0016897384462797	5.067506865096336E-4	0.0014768162863995037	5.791436417252956E-6	2.60614638776383E-5
>>END_MODULE
