##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654110_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39490969
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.385793470907235	32.0	32.0	32.0	32.0	32.0
2	31.454114686322335	32.0	32.0	32.0	32.0	32.0
3	34.87720225350763	37.0	32.0	37.0	32.0	37.0
4	35.90108039638126	37.0	37.0	37.0	32.0	37.0
5	36.063237394858554	37.0	37.0	37.0	37.0	37.0
6	39.361340234522984	41.0	41.0	41.0	37.0	41.0
7	39.61072778437014	41.0	41.0	41.0	37.0	41.0
8	39.70557595079523	41.0	41.0	41.0	37.0	41.0
9	39.70459982888746	41.0	41.0	41.0	37.0	41.0
10-11	39.6608715780056	41.0	41.0	41.0	37.0	41.0
12-13	39.696785447325944	41.0	41.0	41.0	37.0	41.0
14-15	39.66526622833692	41.0	41.0	41.0	37.0	41.0
16-17	39.59195834115896	41.0	41.0	41.0	37.0	41.0
18-19	39.572639240632455	41.0	41.0	41.0	37.0	41.0
20-21	39.61342362857695	41.0	41.0	41.0	37.0	41.0
22-23	39.53587760786523	41.0	41.0	41.0	37.0	41.0
24-25	39.499957610561545	41.0	41.0	41.0	37.0	41.0
26-27	39.43591313244301	41.0	41.0	41.0	37.0	41.0
28-29	39.34693725798422	41.0	41.0	41.0	37.0	41.0
30-31	39.471129120685795	41.0	41.0	41.0	37.0	41.0
32-33	39.541044346113665	41.0	41.0	41.0	37.0	41.0
34-35	39.48751539877384	41.0	41.0	41.0	37.0	41.0
36-37	39.333220223084425	41.0	41.0	41.0	37.0	41.0
38-39	39.253006554485914	41.0	41.0	41.0	37.0	41.0
40-41	39.24176483235952	41.0	41.0	41.0	37.0	41.0
42-43	39.205540145140525	41.0	41.0	41.0	37.0	41.0
44-45	39.195203376751785	41.0	41.0	41.0	37.0	41.0
46-47	39.143965421562584	41.0	41.0	41.0	37.0	41.0
48-49	39.11486968071105	41.0	41.0	41.0	37.0	41.0
50-51	39.11347819801534	41.0	41.0	41.0	37.0	41.0
52-53	39.124460354467374	41.0	41.0	41.0	37.0	41.0
54-55	39.124374512056164	41.0	41.0	41.0	37.0	41.0
56-57	39.08531022624439	41.0	41.0	41.0	37.0	41.0
58-59	39.052800021189654	41.0	41.0	41.0	37.0	41.0
60-61	39.00675851990363	41.0	41.0	41.0	37.0	41.0
62-63	38.946554805986146	41.0	41.0	41.0	37.0	41.0
64-65	38.96615103316407	41.0	41.0	41.0	37.0	41.0
66-67	38.958119703266846	41.0	41.0	41.0	37.0	41.0
68-69	38.903055139011656	41.0	41.0	41.0	34.5	41.0
70-71	38.828037696416104	41.0	41.0	41.0	32.0	41.0
72-73	38.84133683830346	41.0	41.0	41.0	32.0	41.0
74-75	38.74032927629605	41.0	41.0	41.0	32.0	41.0
76-77	37.82600429480472	41.0	39.0	41.0	29.5	41.0
78-79	38.37510286212526	41.0	39.0	41.0	32.0	41.0
80-81	38.84838400648006	41.0	41.0	41.0	32.0	41.0
82-83	38.925632199604934	41.0	41.0	41.0	32.0	41.0
84-85	38.8938777749414	41.0	41.0	41.0	32.0	41.0
86-87	38.84513269350266	41.0	41.0	41.0	32.0	41.0
88-89	38.771426069590746	41.0	41.0	41.0	32.0	41.0
90-91	38.65573844237653	41.0	41.0	41.0	32.0	41.0
92-93	38.570603737274716	41.0	41.0	41.0	32.0	41.0
94-95	38.43382473091506	41.0	39.0	41.0	32.0	41.0
96-97	38.46680609432501	41.0	39.0	41.0	32.0	41.0
98-99	38.40696679283813	41.0	37.0	41.0	32.0	41.0
100-101	37.340649655874486	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	31.0
13	160.0
14	558.0
15	2303.0
16	9122.0
17	24145.0
18	39401.0
19	52307.0
20	63525.0
21	76041.0
22	90187.0
23	108152.0
24	126588.0
25	148601.0
26	172024.0
27	200267.0
28	233002.0
29	272218.0
30	319255.0
31	379746.0
32	448285.0
33	533067.0
34	652851.0
35	820071.0
36	1079480.0
37	1538291.0
38	2579953.0
39	6604913.0
40	2.2916425E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.247651484638574	20.425705742431735	12.261036353800845	31.065606419128848
2	30.148086942732245	22.968764252692086	29.881353785238986	17.001795019336686
3	22.11569983073992	26.123564364739387	28.819717733078765	22.941018071441924
4	24.977331799827617	31.561713708083012	22.667911494210117	20.793042997879255
5	26.784850478175237	32.96994035859996	21.669833602243678	18.575375560981115
6	21.862767476206063	36.00091941048538	22.034965757099894	20.101347356208663
7	21.45724254069329	19.838999139271564	37.37658349178517	21.327174828249973
8	22.86084528435085	24.033203858137277	26.344786445964125	26.761164411547746
9	23.170755825211582	23.263571982748765	28.674515431616783	24.89115676042287
10-11	25.49104581353777	29.252608362180222	22.644510191684585	22.61183563259742
12-13	25.74446871227706	23.908862334535673	25.986980608443233	24.359688344744033
14-15	24.407423378241237	26.37112424362137	26.492035432202233	22.729416945935156
16-17	25.27751392415718	26.06042292507464	25.74994351178932	22.912119638978865
18-19	24.902823984896393	26.623460416987992	25.790872591655067	22.68284300646054
20-21	25.06191565847028	26.514336826347833	25.74436163869789	22.679385876483995
22-23	25.11284352411924	26.37074254548534	25.81038403515457	22.706029895240857
24-25	24.72176957647539	26.344800374394513	26.090067415042938	22.843362634087164
26-27	24.82171204877224	26.5297039804203	26.00371256625999	22.644871404547473
28-29	25.044116533055266	26.258321272334804	25.86532375739577	22.832238437214162
30-31	24.763902546045045	26.30779382670882	26.107172903851055	22.821130723395083
32-33	24.774744216832627	26.561945242246843	25.977813855403642	22.685496685516892
34-35	25.05166434381491	26.434312108168328	25.771125038739868	22.742898509276895
36-37	24.761557509515654	26.273599414590205	26.14234155662273	22.822501519271405
38-39	24.90043806731272	26.474318123211056	25.944870883960913	22.680372925515314
40-41	25.1010477863939	26.297551726320012	25.818119327484723	22.783281159801373
42-43	24.68225709101775	26.399597061892326	26.160923261148444	22.757222585941477
44-45	24.800540113633485	26.385459575378036	26.073436659621585	22.740563651366898
46-47	25.132354944240543	26.250504260860247	25.86723182204012	22.749908972859085
48-49	24.776781525875172	26.22672573629196	26.130069204000662	22.866423533832204
50-51	24.892505134970342	26.335584178986277	25.953793534752457	22.818117151290927
52-53	25.12808358065411	26.34305137656716	25.866578645981807	22.66228639679692
54-55	24.67619366187157	26.30563445001874	26.22244396784554	22.795727920264156
56-57	24.73012813150088	26.427762921313587	26.148489701514325	22.693619245671204
58-59	25.113399134376195	26.202005755201448	25.912332574711904	22.772262535710457
60-61	24.852082003862705	26.27467181167421	26.105505286537788	22.767740897925297
62-63	24.880854058086783	26.383152016708017	26.057582185936738	22.678411739268466
64-65	25.04945402896361	26.31170719451968	25.986375846923078	22.652462929593636
66-67	24.790043857781	26.334869961985735	26.24050662657812	22.634579553655144
68-69	24.857836066493675	26.519920954860105	26.0617984119006	22.560444566745623
70-71	25.240058195619277	26.3716695055031	25.774145186953618	22.614127111924013
72-73	24.850555129782364	26.341641665021033	26.232627944252624	22.575175260943976
74-75	24.77558819963795	26.61279496236301	26.08730591572264	22.524310922276396
76-77	25.183091206701953	26.398445289979545	25.837221429366007	22.581242073952495
78-79	24.864516491549637	26.463230057707126	26.11288204757876	22.559371403164473
80-81	25.08821115529591	26.49470236744169	26.053461419040868	22.363625058221533
82-83	25.325406336600203	26.258363861715594	25.893528759549255	22.52270104213494
84-85	25.071162219778103	26.432273090326603	26.07399432545711	22.42257036443818
86-87	25.126709349674353	26.68442498840684	25.82204047715314	22.366825184765663
88-89	25.393532379516948	26.521609282365294	25.699936610823602	22.384921727294156
90-91	25.057910075592222	26.540792149212646	25.991445031394395	22.40985274380074
92-93	25.27618909528404	26.681328077819515	25.758427198886913	22.284055628009533
94-95	25.627055447431612	26.64120564041699	25.57747572794883	22.154263184202566
96-97	25.43002089081329	26.5322622469658	25.845694794779973	22.19202206744094
98-99	25.5968893242008	26.810628865686088	25.54878845273192	22.043693357381194
100-101	25.94085585000947	26.61099950490983	25.417454018040388	22.030690627040308
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	79.0
1	60.5
2	39.0
3	46.5
4	56.5
5	63.0
6	81.0
7	118.5
8	134.5
9	155.0
10	213.0
11	247.0
12	286.5
13	348.0
14	423.0
15	527.5
16	651.5
17	800.0
18	963.5
19	1253.5
20	1613.0
21	2059.0
22	2626.5
23	3406.5
24	4508.5
25	5944.0
26	8034.0
27	11120.5
28	15089.5
29	20515.5
30	28569.5
31	40345.0
32	57976.0
33	84659.5
34	127048.0
35	194262.0
36	291646.5
37	431143.0
38	634283.0
39	902646.0
40	1199196.5
41	1509010.0
42	1844931.5
43	2137949.0
44	2355801.0
45	2502464.5
46	2571932.5
47	2577359.5
48	2494974.0
49	2349060.0
50	2144377.5
51	1925126.5
52	1723591.5
53	1524952.0
54	1336215.0
55	1144454.5
56	952394.0
57	769219.0
58	611993.5
59	493361.5
60	418542.5
61	365084.5
62	314443.5
63	266031.5
64	226153.0
65	192956.5
66	165331.5
67	139233.0
68	106856.0
69	77874.0
70	58092.5
71	42827.0
72	28858.5
73	18227.0
74	10368.5
75	5341.5
76	2950.0
77	1690.0
78	1113.5
79	821.5
80	677.5
81	552.5
82	443.5
83	359.5
84	300.0
85	255.0
86	209.0
87	180.5
88	153.5
89	126.5
90	112.5
91	112.5
92	103.5
93	95.5
94	90.0
95	78.0
96	76.0
97	83.5
98	105.5
99	175.0
100	814.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017679991595040374
2	0.007128212022348705
3	6.659750486244083E-4
4	0.0015851725491972607
5	2.8360914618225753E-4
6	0.0011319043602095456
7	0.0
8	0.024139696344245187
9	0.0
10-11	0.0
12-13	0.003965463597512636
14-15	0.0
16-17	0.0010837920943393413
18-19	0.0
20-21	3.823659024421507E-4
22-23	2.532224519484442E-6
24-25	1.3927234857164433E-5
26-27	0.0053467920728914
28-29	9.837692258197058E-4
30-31	1.8485238992236428E-4
32-33	4.963160058189507E-4
34-35	0.0
36-37	0.0
38-39	8.82480245040328E-4
40-41	0.0
42-43	1.025550930391199E-4
44-45	0.0019257567470679184
46-47	0.0
48-49	0.0015750436511193229
50-51	0.0012104033203135633
52-53	6.368544666503373E-4
54-55	6.92563406078995E-4
56-57	0.008342413679441494
58-59	6.520478137672439E-4
60-61	0.0
62-63	0.004807428250241214
64-65	1.266112259742221E-6
66-67	0.0026714968680560864
68-69	2.9373804426019527E-4
70-71	6.077338846762661E-5
72-73	1.7345737958468429E-4
74-75	5.862099762606484E-4
76-77	3.3425363657194637E-4
78-79	2.342307680523109E-4
80-81	0.0016218898047297853
82-83	0.005148012448111871
84-85	5.342993736112173E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	5.9507276207884396E-5
96-97	0.004661825340370858
98-99	1.266112259742221E-6
100-101	4.6213097480591074E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.9490969E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.059228023481783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.4044855498176	18.457714328065595
2	15.103037523985833	9.079712975613838
3	7.021915678418557	6.332200936177356
4	4.041887726043777	4.859840992098487
5	2.5992545900424777	3.906579320658425
6	1.7968462973395505	3.24070875449271
7	1.295553240308984	2.726033118690591
8	0.9842697261182898	2.366911050719971
9	0.7588722028046825	2.0530001326309075
>10	4.505254241701086	24.745353060245296
>50	0.2977788407257709	6.131739931806898
>100	0.1692884768150277	9.603875564859997
>500	0.014825439378750728	3.0378381281506095
>1k	0.006624452603991379	3.2504243658121053
>5k	1.0601389582431568E-4	0.20806733997710908
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.3047816831235514E-5	0.0	2.532224519484442E-6	0.0	0.0
2	5.5708939428657724E-5	1.5193347116906653E-5	1.7725571636391096E-5	1.0128898077937768E-5	0.0
3	7.849896010401771E-5	1.5193347116906653E-5	2.7854469714328862E-5	1.0128898077937768E-5	2.532224519484442E-6
4	1.494012466495821E-4	1.5193347116906653E-5	5.064449038968884E-5	1.0128898077937768E-5	5.064449038968884E-6
5	1.8232016540287984E-4	1.5193347116906653E-5	5.3176714909173284E-5	1.0128898077937768E-5	5.064449038968884E-6
6	2.279002067535998E-4	1.5193347116906653E-5	8.356340914298659E-5	1.0128898077937768E-5	5.064449038968884E-6
7	2.4815800290947535E-4	1.5193347116906653E-5	9.875675625989324E-5	1.266112259742221E-5	5.064449038968884E-6
8	3.013347178186486E-4	1.5193347116906653E-5	1.0382120529886213E-4	1.5193347116906653E-5	7.596673558453327E-6
9	3.3931808561091524E-4	1.7725571636391096E-5	1.1141787885731545E-4	1.7725571636391096E-5	1.266112259742221E-5
10-11	4.216153824941596E-4	2.0257796155875536E-5	1.31675675013191E-4	2.0257796155875536E-5	2.40561329351022E-5
12-13	5.013804548579195E-4	2.0257796155875536E-5	1.506673589093243E-4	2.0257796155875536E-5	2.6588357454586642E-5
14-15	5.798794149619373E-4	2.279002067535998E-5	1.7598960410416872E-4	2.40561329351022E-5	2.7854469714328862E-5
16-17	6.811683957413149E-4	2.40561329351022E-5	2.6841579906535087E-4	2.7854469714328862E-5	2.7854469714328862E-5
18-19	8.19174632053217E-4	2.532224519484442E-5	2.9880249329916413E-4	3.165280649355553E-5	2.7854469714328862E-5
20-21	9.698419909625413E-4	3.0386694233813306E-5	3.3298752431220414E-4	3.291891875329775E-5	2.7854469714328862E-5
22-23	0.00120280664675511	3.418503101303997E-5	3.6210810628627523E-4	3.545114327278219E-5	2.7854469714328862E-5
24-25	0.0015877047737167453	4.431392909097774E-5	3.7223700436421296E-4	3.545114327278219E-5	2.9120581974071086E-5
26-27	0.0020599646466005934	4.558004135071996E-5	3.937609127798307E-4	3.545114327278219E-5	3.545114327278219E-5
28-29	0.002570207887276709	5.064449038968884E-5	4.0515592311751074E-4	3.545114327278219E-5	3.924948005200885E-5
30-31	0.0031868045577711705	5.697505168839995E-5	4.2541371927338627E-4	3.545114327278219E-5	4.1781704571493297E-5
32-33	0.003989519730447739	6.203950072736884E-5	4.330103928318396E-4	3.545114327278219E-5	4.558004135071996E-5
34-35	0.004928975027176466	6.58378375065955E-5	4.912515567799818E-4	3.545114327278219E-5	5.064449038968884E-5
36-37	0.006255860675386315	6.710394976633772E-5	5.089771284163729E-4	3.545114327278219E-5	6.203950072736884E-5
38-39	0.00809425567653202	6.837006202607993E-5	5.241704755332794E-4	3.545114327278219E-5	6.58378375065955E-5
40-41	0.010644205767652851	6.837006202607993E-5	5.520249452476084E-4	3.6717255532524415E-5	6.837006202607993E-5
42-43	0.014129812818723188	6.963617428582217E-5	6.064677724165238E-4	3.798336779226663E-5	7.21683988053066E-5
44-45	0.018147187018885257	7.723284784427548E-5	6.216611195334306E-4	3.798336779226663E-5	7.72328478442755E-5
46-47	0.022450702589749065	9.116008270143992E-5	6.621767118451815E-4	3.798336779226663E-5	7.976507236375993E-5
48-49	0.028024128757134323	9.495841948066659E-5	6.87498957040026E-4	3.798336779226663E-5	8.229729688324436E-5
50-51	0.03566511624467863	1.0508731755860435E-4	7.064906409361593E-4	3.798336779226663E-5	8.356340914298659E-5
52-53	0.0453774633891612	1.1521621563654211E-4	7.343451106504883E-4	4.1781704571493297E-5	8.862785818195547E-5
54-55	0.05668384586865924	1.1901455241576878E-4	7.710623661830126E-4	4.9378378129946626E-5	9.242619496118214E-5
56-57	0.06897652979849647	1.2407900145473767E-4	8.343679791701236E-4	5.064449038968884E-5	9.369230722092436E-5
58-59	0.08314812432179114	1.2914345049370654E-4	8.723513469623903E-4	5.064449038968884E-5	9.495841948066659E-5
60-61	0.10140293088275448	1.3040956275344877E-4	9.153991637936258E-4	5.3176714909173284E-5	9.62245317404088E-5
62-63	0.12535650872481757	1.31675675013191E-4	9.900997871184168E-4	5.3176714909173284E-5	9.875675625989324E-5
64-65	0.15486072271359053	1.3547401179241764E-4	0.0010382120529886215	5.3176714909173284E-5	1.0888565433783101E-4
66-67	0.18945470798652725	1.4686902213009766E-4	0.0010445426142873322	5.3176714909173284E-5	1.1141787885731545E-4
68-69	0.2293663647503813	1.54465695688551E-4	0.0010470748388068168	5.3176714909173284E-5	1.1141787885731545E-4
70-71	0.2778685931965863	1.5699792020803542E-4	0.0010559376246250124	5.3176714909173284E-5	1.1268399111705768E-4
72-73	0.33938392344842183	1.6459459376648873E-4	0.0010685987472224346	5.3176714909173284E-5	1.2407900145473767E-4
74-75	0.41799683365581636	1.6459459376648873E-4	0.0010749293085211458	5.3176714909173284E-5	1.266112259742221E-4
76-77	0.5156102905451624	1.6459459376648873E-4	0.0010913887678977946	5.3176714909173284E-5	1.266112259742221E-4
78-79	0.6339829240452418	1.7345737958468429E-4	0.0011002515537159902	5.444282716891551E-5	1.278773382339643E-4
80-81	0.774488465957875	1.7978794088339538E-4	0.001121775462131608	5.697505168839995E-5	1.31675675013191E-4
82-83	0.9437588122995919	1.8991683896133315E-4	0.0011319043602095458	5.824116394814217E-5	1.3294178727293323E-4
84-85	1.1547830087430877	1.924490634808176E-4	0.0011382349215082568	5.824116394814217E-5	1.3547401179241764E-4
86-87	1.4173721591891049	1.937151757405598E-4	0.0011470977073264523	5.824116394814217E-5	1.3674012405215987E-4
88-89	1.730781789628915	1.9751351251978648E-4	0.0011483638195861944	5.950727620788439E-5	1.392723485716443E-4
90	1.9999104098965006	2.2030353319514646E-4	0.0011496299318459367	6.077338846762661E-5	1.4180457309112877E-4
>>END_MODULE
