##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654118_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24512826
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.314923216115513	32.0	32.0	32.0	32.0	32.0
2	31.435720304137924	32.0	32.0	32.0	32.0	32.0
3	34.78184999966956	37.0	32.0	37.0	32.0	37.0
4	35.82074612694595	37.0	37.0	37.0	32.0	37.0
5	35.94386522386281	37.0	37.0	37.0	37.0	37.0
6	39.243767120119074	41.0	41.0	41.0	37.0	41.0
7	39.472874078247855	41.0	41.0	41.0	37.0	41.0
8	39.587026807924964	41.0	41.0	41.0	37.0	41.0
9	39.581037983951745	41.0	41.0	41.0	37.0	41.0
10-11	39.53674013351215	41.0	41.0	41.0	37.0	41.0
12-13	39.57615525439621	41.0	41.0	41.0	37.0	41.0
14-15	39.509574028714596	41.0	41.0	41.0	37.0	41.0
16-17	39.46691750269838	41.0	41.0	41.0	37.0	41.0
18-19	39.483118082753904	41.0	41.0	41.0	37.0	41.0
20-21	39.4790324053212	41.0	41.0	41.0	37.0	41.0
22-23	39.27478555512123	41.0	41.0	41.0	37.0	41.0
24-25	39.304934445338944	41.0	41.0	41.0	37.0	41.0
26-27	39.3061161736309	41.0	41.0	41.0	37.0	41.0
28-29	39.26311042227445	41.0	41.0	41.0	37.0	41.0
30-31	39.29447997958293	41.0	41.0	41.0	37.0	41.0
32-33	39.39708316372825	41.0	41.0	41.0	37.0	41.0
34-35	39.35286773952542	41.0	41.0	41.0	37.0	41.0
36-37	39.20218886635102	41.0	41.0	41.0	37.0	41.0
38-39	39.13380382988073	41.0	41.0	41.0	37.0	41.0
40-41	39.05953526125466	41.0	41.0	41.0	37.0	41.0
42-43	39.04286372366858	41.0	41.0	41.0	37.0	41.0
44-45	39.04639964808627	41.0	41.0	41.0	37.0	41.0
46-47	38.99681560583835	41.0	41.0	41.0	37.0	41.0
48-49	38.95221889144891	41.0	41.0	41.0	37.0	41.0
50-51	39.00001429863697	41.0	41.0	41.0	37.0	41.0
52-53	38.99593425091011	41.0	41.0	41.0	37.0	41.0
54-55	38.98816317628983	41.0	41.0	41.0	37.0	41.0
56-57	38.94857734885402	41.0	41.0	41.0	37.0	41.0
58-59	38.8522285435388	41.0	41.0	41.0	32.0	41.0
60-61	38.892946696557956	41.0	41.0	41.0	32.0	41.0
62-63	38.885623305937884	41.0	41.0	41.0	32.0	41.0
64-65	38.86140098656924	41.0	41.0	41.0	32.0	41.0
66-67	38.820738967428724	41.0	41.0	41.0	32.0	41.0
68-69	38.7920357897535	41.0	41.0	41.0	32.0	41.0
70-71	38.76177893564781	41.0	41.0	41.0	32.0	41.0
72-73	38.733697146954825	41.0	41.0	41.0	32.0	41.0
74-75	38.70781404396213	41.0	41.0	41.0	32.0	41.0
76-77	37.851332706396235	41.0	39.0	41.0	29.5	41.0
78-79	38.309709537366274	41.0	39.0	41.0	32.0	41.0
80-81	38.64742200674863	41.0	41.0	41.0	32.0	41.0
82-83	38.80880756058073	41.0	41.0	41.0	32.0	41.0
84-85	38.76049607662536	41.0	41.0	41.0	32.0	41.0
86-87	38.69661635504613	41.0	41.0	41.0	32.0	41.0
88-89	38.63527693624554	41.0	41.0	41.0	32.0	41.0
90-91	38.581692682026954	41.0	41.0	41.0	32.0	41.0
92-93	38.511177107853655	41.0	41.0	41.0	32.0	41.0
94-95	38.49823229275972	41.0	41.0	41.0	32.0	41.0
96-97	38.40260557881005	41.0	37.0	41.0	32.0	41.0
98-99	38.194484838263854	41.0	37.0	41.0	32.0	41.0
100-101	37.214553882934595	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	19.0
13	89.0
14	305.0
15	1201.0
16	5393.0
17	14670.0
18	26024.0
19	35939.0
20	44489.0
21	53761.0
22	64747.0
23	76138.0
24	89794.0
25	104146.0
26	120174.0
27	139863.0
28	161967.0
29	187407.0
30	218379.0
31	257100.0
32	300868.0
33	356885.0
34	430101.0
35	534502.0
36	692728.0
37	973448.0
38	1612032.0
39	4037447.0
40	1.397321E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.329018904287494	17.55818024750279	13.918423033208418	41.194377815001296
2	27.731559796491844	19.20780166268875	35.97083012786857	17.089808412950834
3	21.165575283186058	22.586724283435768	30.675352859943906	25.57234757343427
4	24.104764583243075	29.779601911260663	23.34483588305975	22.770797622436515
5	27.090182032885508	31.9778198418848	22.603633770932053	18.328364354297644
6	20.647223620809775	35.4003165526488	23.078844520007607	20.873615306533814
7	20.899414045528655	18.129382552627753	38.826396434258534	22.14480696758505
8	21.801088948291806	22.888058684053807	27.87773225331098	27.433120114343406
9	22.759999193891396	22.405458269071058	29.74148717083865	25.093055366198904
10-11	25.66124100530124	28.314441549083064	22.588730478951952	23.435586966663745
12-13	26.334839205246553	22.447094688718412	25.386692266448396	25.83137383958664
14-15	24.345752709214352	26.329905821548277	26.536275336022047	22.78806613321532
16-17	25.577497441158165	25.947946388043725	25.428112765644233	23.046443405153877
18-19	25.201395147661014	26.76709847817371	25.28071487498326	22.75079149918201
20-21	25.48275580790602	26.62954760108737	25.362859692171785	22.524836898834817
22-23	25.179919244828408	26.49473792875854	25.648679695391785	22.67666313102127
24-25	24.70677353456427	26.511168304251097	26.000140461416365	22.78191769976827
26-27	24.961852623602027	26.512897778493596	25.98351369197497	22.54173590592941
28-29	25.13349029963282	26.352998017156143	25.791130384181503	22.722381299029532
30-31	24.9252268995831	26.490325921621604	25.81325996439578	22.771187214399514
32-33	25.00406184381334	26.683677352380762	25.725418787061326	22.58684201674457
34-35	25.208617317317884	26.44775637048131	25.661025375042435	22.682600937158366
36-37	24.99395418545377	26.393539855420993	25.8614836983708	22.751022260754432
38-39	25.155226981513874	26.677072212405474	25.65667626710474	22.51102453897591
40-41	25.354528686329353	26.42121720278192	25.517245543210727	22.707008567678
42-43	25.01449355770488	26.502680354584772	25.858997499858745	22.6238285878516
44-45	25.162141983919224	26.45384595959771	25.88938367708618	22.494628379396882
46-47	25.230784318900945	26.434675543048865	25.723216941441322	22.611323196608865
48-49	24.944172853902963	26.427946892793543	25.921527563955397	22.706352689348094
50-51	25.28522823113092	26.442444865394144	25.683811405506653	22.588515497968288
52-53	25.490922252536592	26.373811257069384	25.596774367261656	22.538492123132368
54-55	24.9970734564571	26.47194533463473	25.840638031571018	22.69034317733716
56-57	25.06863945612506	26.58307372705449	25.93574887131112	22.41253794550933
58-59	25.338396315463584	26.29169113344989	25.768589472303194	22.601323078783327
60-61	25.182241902629105	26.331171437147116	25.77290635807165	22.713680302152127
62-63	25.233291895119436	26.49641220568084	25.767356930051715	22.502938969148005
64-65	25.308218236444873	26.452861860970252	25.691007638205406	22.547912264379473
66-67	25.06987841451645	26.58364181553698	25.891642041873077	22.454837728073496
68-69	25.325826701267474	26.692547511243546	25.769502402099416	22.212123385389564
70-71	25.60006817854383	26.46168921276689	25.487028136561314	22.45121447212797
72-73	25.180085315336555	26.553864087314942	25.85506868934655	22.410981908001958
74-75	25.112487328538798	26.66056263511075	25.824512383570337	22.402437652780115
76-77	25.42738911858985	26.475739325840863	25.681608056970596	22.41526349859869
78-79	25.150717016471297	26.581127365730907	25.884991799803093	22.38316381799471
80-81	25.340759656940126	26.60722444680707	25.83784036061467	22.21417553563813
82-83	25.60863947059218	26.352553177874956	25.69813863082746	22.340668720705402
84-85	25.336141167143154	26.465461422790415	25.880006492294665	22.318390917771765
86-87	25.374491704873197	26.674931319628342	25.727094868621027	22.223482106877437
88-89	25.510269645637855	26.576289667439724	25.630034685456803	22.283406001465615
90-91	25.238839338620284	26.594564794409393	25.899499841246314	22.267096025724012
92-93	25.478751244761394	26.66930815131566	25.745155680334257	22.10678492358868
94-95	25.702750470304814	26.443315838002523	25.702544455706576	22.15138923598609
96-97	25.41357625318989	26.519922078071485	25.869114217977828	22.197387450760797
98-99	25.55233143737289	26.826324088890964	25.677506764012197	21.943837709723947
100-101	25.775647907500975	26.547705825133878	25.675550422957922	22.001095844407228
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	14.5
2	14.5
3	20.0
4	24.5
5	39.5
6	58.5
7	64.5
8	80.0
9	102.0
10	120.5
11	146.5
12	192.5
13	237.5
14	291.0
15	364.5
16	429.0
17	563.0
18	751.5
19	936.5
20	1193.0
21	1543.5
22	2022.5
23	2648.0
24	3529.0
25	4805.5
26	6440.0
27	8571.0
28	11652.5
29	16080.0
30	21775.0
31	29572.5
32	41274.5
33	58782.5
34	85540.0
35	127528.0
36	188269.5
37	271539.0
38	390629.5
39	547986.5
40	726104.0
41	916968.5
42	1123748.0
43	1304031.5
44	1442437.0
45	1537487.5
46	1575901.5
47	1576910.0
48	1531040.0
49	1435411.5
50	1307821.0
51	1181133.5
52	1062637.5
53	937083.0
54	815517.0
55	705582.5
56	590871.0
57	482892.5
58	391239.5
59	318156.5
60	271041.5
61	236069.5
62	204634.5
63	172268.5
64	149760.5
65	133999.0
66	116047.5
67	99715.5
68	80635.5
69	61440.0
70	51832.5
71	43946.0
72	33131.0
73	24993.0
74	17118.0
75	10499.0
76	6488.0
77	3329.0
78	1897.0
79	1160.5
80	725.5
81	511.5
82	364.0
83	252.0
84	208.5
85	210.5
86	182.0
87	150.5
88	126.5
89	100.5
90	82.5
91	72.5
92	58.0
93	47.5
94	42.5
95	41.5
96	36.5
97	36.0
98	39.5
99	69.5
100	349.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04240637126049848
2	0.0
3	2.2437233471163222E-4
4	0.0
5	0.008526148719042023
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.2029283771687522E-4
12-13	4.7730114838656304E-4
14-15	0.0
16-17	0.0011075834340765116
18-19	3.467572445543406E-5
20-21	0.0014502611816360953
22-23	3.7735347201501776E-4
24-25	0.003086139476533632
26-27	0.0
28-29	0.0022763593230743775
30-31	0.0
32-33	0.0047913692203420365
34-35	0.0
36-37	0.0
38-39	0.002351830017477381
40-41	0.0
42-43	8.607738658937161E-4
44-45	8.362968839251745E-4
46-47	0.0017827401867087866
48-49	0.0013605122477514423
50-51	0.0
52-53	0.003255438601816045
54-55	4.915793878682124E-4
56-57	0.0035389636429516533
58-59	0.0
60-61	3.467572445543406E-5
62-63	0.0033859825056482677
64-65	0.0
66-67	0.0024762546758174677
68-69	4.1610869346520875E-4
70-71	0.002918880099748597
72-73	0.0
74-75	2.651673046592017E-4
76-77	0.0036042355948677643
78-79	0.0
80-81	0.004126411210196654
82-83	0.001246286331898248
84-85	0.00282097217187443
86-87	0.0
88-89	0.0010076357577049663
90-91	3.6715472952812544E-5
92-93	3.9163171149666706E-4
94-95	0.0
96-97	0.0024436186998594124
98-99	3.263597595805559E-5
100-101	9.056483328360427E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.4512826E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.76371118747551
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.2257380419738	22.32725008093636
2	15.01254304673225	10.43783421332287
3	6.610338574537515	6.893997031699521
4	3.687302613195672	5.127372924238322
5	2.291324059516545	3.9827463920973547
6	1.5708657974035936	3.2765474937133163
7	1.1031810835740485	2.6845468003798145
8	0.7994826745692065	2.223438783849149
9	0.6368398386431181	1.9925024600941075
>10	3.6698243415593126	23.19389723033001
>50	0.251079486605196	5.970659075448069
>100	0.1306156813049192	8.324060724958212
>500	0.00785086389176494	1.8383474545402039
>1k	0.0029771696259259972	1.6491849601759023
>5k	3.6726867117605516E-5	0.07761437421685856
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.079496994756949E-6	8.158993989513898E-6	4.079496994756949E-6	0.0	0.0
2	4.079496994756949E-6	2.855647896329864E-5	8.158993989513898E-6	0.0	0.0
3	1.2238490984270847E-5	2.855647896329864E-5	1.2238490984270847E-5	0.0	8.158993989513898E-6
4	4.895396393708339E-5	2.855647896329864E-5	4.895396393708339E-5	0.0	8.158993989513898E-6
5	5.711295792659728E-5	3.671547295281254E-5	7.751044290038203E-5	0.0	8.158993989513898E-6
6	8.974893388465287E-5	3.671547295281254E-5	1.1014641885843762E-4	0.0	8.158993989513898E-6
7	1.1422591585319456E-4	3.671547295281254E-5	1.1422591585319456E-4	0.0	8.158993989513898E-6
8	1.2646440683746542E-4	3.671547295281254E-5	1.1422591585319456E-4	0.0	8.158993989513898E-6
9	1.3870289782173627E-4	4.895396393708339E-5	1.1830541284795152E-4	0.0	8.158993989513898E-6
10-11	1.8969661025619814E-4	4.895396393708339E-5	1.2442465834008694E-4	0.0	8.158993989513898E-6
12-13	2.304915802037676E-4	5.3033460931840336E-5	1.3258365232960085E-4	0.0	8.158993989513898E-6
14-15	3.3043925657531283E-4	5.915270642397576E-5	1.6114013129289948E-4	0.0	8.158993989513898E-6
16-17	4.4466517242850747E-4	6.527195191611118E-5	2.488493166801739E-4	0.0	8.158993989513898E-6
18-19	5.58891088281702E-4	7.547069440300356E-5	2.6312755616182324E-4	0.0	8.158993989513898E-6
20-21	7.56746692527414E-4	8.566943688989592E-5	2.79445544140851E-4	0.0	8.158993989513898E-6
22-23	9.811190272390462E-4	8.77091853872744E-5	2.998430291146358E-4	0.0	8.158993989513898E-6
24-25	0.0014074264631911474	9.994767637154525E-5	3.2228026258579895E-4	0.0	8.158993989513898E-6
26-27	0.0019724367969649846	1.0606692186368067E-4	3.4675724455434065E-4	0.0	1.0198742486892373E-5
28-29	0.0026027190826549335	1.1422591585319456E-4	3.671547295281254E-4	0.0	2.0397484973784745E-5
30-31	0.003473691691035542	1.3258365232960085E-4	3.793932205123962E-4	2.0397484973784744E-6	2.4476981968541695E-5
32-33	0.004365061784389935	1.3870289782173627E-4	3.9367145999404557E-4	4.079496994756949E-6	2.4476981968541695E-5
34-35	0.005525678679398287	1.4074264631911473E-4	5.507320942921881E-4	4.079496994756949E-6	2.8556478963298644E-5
36-37	0.006857634448186431	1.427823948164932E-4	5.711295792659728E-4	4.079496994756949E-6	4.283471844494796E-5
38-39	0.008605698910439784	1.4686189181125015E-4	5.792885732554868E-4	4.079496994756949E-6	4.4874466942326436E-5
40-41	0.01081882603009543	1.4686189181125015E-4	6.200835432030562E-4	4.079496994756949E-6	4.4874466942326436E-5
42-43	0.013711189399378106	1.6317987979027796E-4	6.445605251715979E-4	4.079496994756949E-6	4.691421543970491E-5
44-45	0.017021701210623366	1.7337862227717032E-4	6.506797706637333E-4	4.079496994756949E-6	5.711295792659728E-5
46-47	0.02047907491367988	1.7745811927192726E-4	6.894349921139243E-4	8.158993989513898E-6	6.119245492135423E-5
48-49	0.02481558021910652	1.835773647640627E-4	7.343094590562508E-4	8.158993989513898E-6	6.119245492135423E-5
50-51	0.03101845539963446	1.9377610725095507E-4	7.383889560510078E-4	8.158993989513898E-6	6.323220341873271E-5
52-53	0.03885516912656256	1.998953527430905E-4	7.955019139776051E-4	8.158993989513898E-6	6.935144891086813E-5
54-55	0.04749758350995516	2.0397484973784746E-4	8.893303448570149E-4	8.158993989513898E-6	6.935144891086813E-5
56-57	0.056505112874378496	2.100940952299829E-4	9.93357518223317E-4	1.2238490984270847E-5	6.935144891086813E-5
58-59	0.06610620905153898	2.1825308921949677E-4	0.001028033242678751	1.2238490984270847E-5	7.13911974082466E-5
60-61	0.07773481523509367	2.2029283771687525E-4	0.0010647487156315637	1.6317987979027795E-5	7.95501913977605E-5
62-63	0.09217623459653326	2.243723347116322E-4	0.0011442989070293242	1.6317987979027795E-5	8.362968839251745E-5
64-65	0.10943046713585777	2.386505741932815E-4	0.0011728553859926228	1.6317987979027795E-5	1.0810667036105915E-4
66-67	0.1290242912016754	2.5292881367493085E-4	0.0011748951344900012	1.6317987979027795E-5	1.2238490984270846E-4
68-69	0.1514717234153255	2.7740579564347254E-4	0.0011871336254742721	1.6317987979027795E-5	1.2442465834008694E-4
70-71	0.17688291019566654	2.835250411356079E-4	0.001199372116458543	1.6317987979027795E-5	1.2850415533484388E-4
72-73	0.2070854661963496	2.855647896329864E-4	0.0012462863318982477	1.6317987979027795E-5	1.4686189181125015E-4
74-75	0.24476981968541695	2.896442866277434E-4	0.0012524055773903834	1.835773647640627E-5	1.4686189181125015E-4
76-77	0.2911394222763218	2.9780328061725726E-4	0.0012666838168720327	2.0397484973784745E-5	1.4686189181125015E-4
78-79	0.34600865685580273	3.0596227460677114E-4	0.0012830018048510606	2.0397484973784745E-5	1.4890164030862863E-4
80-81	0.40940404015432574	3.120815200989066E-4	0.0013033992898248453	2.243723347116322E-5	1.5298113730338557E-4
82-83	0.483191942046992	3.2228026258579895E-4	0.0013176775293064944	2.4476981968541695E-5	1.5502088580076405E-4
84-85	0.572655311142012	3.263597595805559E-4	0.0013217570263012513	2.4476981968541695E-5	1.5502088580076405E-4
86-87	0.684647702390577	3.324790050726913E-4	0.001331955768788144	2.6516730465920168E-5	1.5706063429814254E-4
88-89	0.821527065055657	3.508367415490976E-4	0.0013421545112750361	2.855647896329864E-5	1.59100382795521E-4
90	0.9404913166682618	3.508367415490976E-4	0.0013503135052645502	2.855647896329864E-5	1.59100382795521E-4
>>END_MODULE
