##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654140_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	34752666
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42796572786675	32.0	32.0	32.0	32.0	32.0
2	31.501999932897235	32.0	32.0	32.0	32.0	32.0
3	34.17213939788101	37.0	32.0	37.0	32.0	37.0
4	35.63534843628975	37.0	37.0	37.0	32.0	37.0
5	35.72753209782525	37.0	37.0	37.0	32.0	37.0
6	39.22167352570879	41.0	41.0	41.0	37.0	41.0
7	39.27527116912412	41.0	41.0	41.0	37.0	41.0
8	39.441534471053245	41.0	41.0	41.0	37.0	41.0
9	39.43854819656138	41.0	41.0	41.0	37.0	41.0
10-11	39.417081915384564	41.0	41.0	41.0	37.0	41.0
12-13	39.59500655287856	41.0	41.0	41.0	37.0	41.0
14-15	39.56603271818052	41.0	41.0	41.0	37.0	41.0
16-17	39.4067650665995	41.0	41.0	41.0	37.0	41.0
18-19	39.48131298761367	41.0	41.0	41.0	37.0	41.0
20-21	39.56330013645572	41.0	41.0	41.0	37.0	41.0
22-23	39.399915692798935	41.0	41.0	41.0	37.0	41.0
24-25	39.370515962717796	41.0	41.0	41.0	37.0	41.0
26-27	39.33913021234112	41.0	41.0	41.0	37.0	41.0
28-29	39.12069289878365	41.0	41.0	41.0	37.0	41.0
30-31	39.304453736009776	41.0	41.0	41.0	37.0	41.0
32-33	39.4511444532054	41.0	41.0	41.0	37.0	41.0
34-35	39.37748037805215	41.0	41.0	41.0	37.0	41.0
36-37	39.125985039536246	41.0	41.0	41.0	37.0	41.0
38-39	39.02863216306916	41.0	41.0	41.0	37.0	41.0
40-41	39.02679485078929	41.0	41.0	41.0	37.0	41.0
42-43	39.00399265771438	41.0	41.0	41.0	37.0	41.0
44-45	39.01039597940486	41.0	41.0	41.0	37.0	41.0
46-47	38.96134578567296	41.0	41.0	41.0	37.0	41.0
48-49	38.944225314972954	41.0	41.0	41.0	37.0	41.0
50-51	38.95581616673668	41.0	41.0	41.0	37.0	41.0
52-53	38.97787328028302	41.0	41.0	41.0	37.0	41.0
54-55	38.981135260241615	41.0	41.0	41.0	37.0	41.0
56-57	38.94133094709914	41.0	41.0	41.0	34.5	41.0
58-59	38.91969027642369	41.0	41.0	41.0	37.0	41.0
60-61	38.875555648018484	41.0	41.0	41.0	32.0	41.0
62-63	38.818382206993846	41.0	41.0	41.0	32.0	41.0
64-65	38.84188379964864	41.0	41.0	41.0	32.0	41.0
66-67	38.83533772632005	41.0	41.0	41.0	32.0	41.0
68-69	38.788345461035995	41.0	41.0	41.0	32.0	41.0
70-71	38.721190181495714	41.0	41.0	41.0	32.0	41.0
72-73	38.731924955052364	41.0	41.0	41.0	32.0	41.0
74-75	38.63382827953401	41.0	41.0	41.0	32.0	41.0
76-77	37.67629641708639	41.0	39.0	41.0	29.5	41.0
78-79	38.241251275513655	41.0	37.0	41.0	32.0	41.0
80-81	38.74513707811654	41.0	41.0	41.0	32.0	41.0
82-83	38.832349466368996	41.0	41.0	41.0	32.0	41.0
84-85	38.79954991078958	41.0	41.0	41.0	32.0	41.0
86-87	38.75360907563178	41.0	41.0	41.0	32.0	41.0
88-89	38.679477655038035	41.0	41.0	41.0	32.0	41.0
90-91	38.55775423099914	41.0	41.0	41.0	32.0	41.0
92-93	38.46974059486544	41.0	39.0	41.0	32.0	41.0
94-95	38.33913799591663	41.0	37.0	41.0	32.0	41.0
96-97	38.36557111906177	41.0	37.0	41.0	32.0	41.0
98-99	38.3055522560485	41.0	37.0	41.0	32.0	41.0
100-101	37.22053247655877	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	34.0
13	138.0
14	500.0
15	2153.0
16	7282.0
17	17538.0
18	30156.0
19	41908.0
20	52805.0
21	65457.0
22	79803.0
23	95819.0
24	113902.0
25	133830.0
26	156391.0
27	182840.0
28	213669.0
29	250177.0
30	293783.0
31	348111.0
32	415662.0
33	504503.0
34	623543.0
35	792556.0
36	1054149.0
37	1529137.0
38	2649389.0
39	7133918.0
40	1.7963513E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	33.37251028315789	20.82223417286434	11.192046236897504	34.61320930708027
2	29.037224661775475	25.33451088000463	29.299110811256853	16.32915364696305
3	21.29803896641614	27.84195952404952	28.339671353714195	22.520330155820144
4	25.13985475305885	31.255353988990485	22.69356988404105	20.911221373909612
5	27.969386540391834	32.78923044425191	21.248306995079528	17.993076020276725
6	22.767498109115508	37.108803443114695	21.40341684302213	18.72028160474766
7	22.463853564500635	21.84426368900734	35.34281082205319	20.34907192443883
8	23.315623068295864	24.742391238931948	26.665408329209626	25.27657736356256
9	23.80395794670832	23.508334583597126	29.083601816332592	23.604105653361962
10-11	25.350686764578008	30.24304092238564	22.880529511030893	21.525742802005464
12-13	25.93255013692506	25.04648997605371	25.514975932488987	23.505983954532244
14-15	24.94885716105924	26.939170652404048	25.62662242948498	22.485349757051733
16-17	25.82353955498044	26.36705477775315	25.09889383556495	22.710511831701464
18-19	25.377322131199946	26.790875554698452	25.293109886878895	22.538692427222706
20-21	25.612316167174292	26.848214586951325	25.162233860222994	22.377235385651392
22-23	25.575648290920306	26.678475928702504	25.170229028907148	22.575646751470043
24-25	25.051040336897373	26.71259226325996	25.59894515126403	22.637422248578638
26-27	25.2749354209056	26.853220085858748	25.453682400435305	22.418162092800348
28-29	25.55027534647233	26.49294659046587	25.279792580098242	22.676985482963556
30-31	25.047600766009047	26.71753375978402	25.61720432248622	22.61766115172071
32-33	25.223854748988355	26.876980352047465	25.401818916546194	22.497345982417986
34-35	25.449246109636597	26.56113490688743	25.33823016628422	22.65138881719175
36-37	25.117353586628433	26.61708025508029	25.649629297504827	22.61593686078645
38-39	25.27911677213269	26.866975065035536	25.324368394069978	22.529539768761794
40-41	25.553733057486873	26.484333604794518	25.27910376717573	22.682829570542875
42-43	25.17510252962372	26.595265182595195	25.60641941465222	22.62321287312886
44-45	25.308028785404	26.71286642238807	25.445428121197384	22.533676671010543
46-47	25.475857017703333	26.513419143152934	25.37054711140722	22.640176727736513
48-49	25.100807137634956	26.55727911909854	25.702814885218178	22.63909885804833
50-51	25.359151596729845	26.66619369964768	25.423593472963056	22.551061230659418
52-53	25.558887311637708	26.482091446575673	25.32498865403538	22.63403258775124
54-55	25.15415046035656	26.572358578872656	25.698188988907532	22.575301971863247
56-57	25.138657947786115	26.742571544675137	25.568999532399122	22.549770975139626
58-59	25.510881408927876	26.319695144274625	25.471628048897514	22.697795397899988
60-61	25.150174090241016	26.4946493601383	25.656992761361096	22.69818378825958
62-63	25.255773703664346	26.64266974322237	25.484067968438012	22.617488584675268
64-65	25.457330747766672	26.433641471328293	25.46347991638224	22.645547864522793
66-67	25.10583752910736	26.563075668241865	25.814638162284425	22.516448640366345
68-69	25.35824005761646	26.76177656446382	25.50256319896001	22.377420178959717
70-71	25.680169500779687	26.518282746412737	25.243402713908736	22.558145038898843
72-73	25.139032361324336	26.60145343613827	25.759679262080184	22.499834940457205
74-75	25.230322484425493	26.777836993394626	25.52160826328627	22.470232258893606
76-77	25.583033139707368	26.467764830054296	25.412943560720834	22.536258469517506
78-79	25.134641456008307	26.5785602975365	25.833313394763696	22.4534848516915
80-81	25.338497034292367	26.725405505978824	25.529652515445335	22.406444944283475
82-83	25.60738252904306	26.428583894862673	25.469660739719057	22.49437283637521
84-85	25.281467512951206	26.6448790461762	25.750840088095643	22.322813352776947
86-87	25.37344904704577	26.844895870722553	25.571651107284833	22.210003974946844
88-89	25.5977901091099	26.653162378966837	25.387565949616643	22.361481562306615
90-91	25.263765375582985	26.75289429593689	25.692979928503902	22.29036039997622
92-93	25.528621314980555	26.839432980479828	25.42687228657508	22.205073417964538
94-95	25.860282001557007	26.561012838015643	25.30873837084918	22.269966789578174
96-97	25.421758356201842	26.660304361333974	25.68310024648046	22.234837035983723
98-99	25.62666596034195	26.914585875434504	25.416512296013664	22.04223586820988
100-101	26.037832934515485	26.608804831687138	25.268091156093426	22.085271077703954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	47.0
1	41.0
2	26.5
3	27.5
4	46.5
5	60.0
6	62.5
7	80.5
8	109.0
9	125.0
10	155.0
11	206.0
12	262.5
13	320.0
14	382.0
15	458.0
16	570.5
17	729.5
18	948.5
19	1189.5
20	1524.5
21	2000.0
22	2611.5
23	3464.5
24	4622.0
25	6345.5
26	8615.5
27	11729.5
28	16519.0
29	22594.0
30	30447.5
31	41749.0
32	58075.5
33	80201.5
34	114399.0
35	169735.0
36	248344.5
37	355964.0
38	511086.0
39	722479.0
40	979630.5
41	1263198.5
42	1566386.5
43	1847498.0
44	2075033.0
45	2232049.5
46	2307603.5
47	2322119.5
48	2257947.5
49	2107966.5
50	1905807.5
51	1713008.5
52	1532457.0
53	1335658.0
54	1149405.5
55	984971.0
56	808744.5
57	651349.5
58	538799.0
59	456527.5
60	390113.5
61	330355.5
62	277453.0
63	230459.5
64	199874.5
65	170955.0
66	143875.0
67	122364.0
68	99058.5
69	76019.0
70	65065.5
71	58921.0
72	46658.0
73	32683.0
74	20172.5
75	12401.5
76	7655.5
77	3958.0
78	2232.5
79	1431.0
80	973.5
81	681.0
82	534.0
83	420.0
84	332.0
85	297.0
86	273.5
87	230.0
88	205.0
89	180.0
90	157.5
91	134.5
92	112.5
93	105.5
94	83.0
95	75.0
96	73.0
97	64.0
98	85.0
99	163.0
100	699.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01739147149171232
2	0.007052696331268513
3	6.531872979183812E-4
4	0.001553837625004079
5	3.1364500208415667E-4
6	0.0011826430812531044
7	0.0
8	0.024196704793813518
9	0.0
10-11	0.0
12-13	0.0039061751406352535
14-15	0.0
16-17	0.001074737690627821
18-19	0.0
20-21	3.8414319062600834E-4
22-23	1.4387385416704433E-6
24-25	1.5826123958374876E-5
26-27	0.005378004668764117
28-29	0.0010344530114610488
30-31	2.1293330416722562E-4
32-33	4.7334498020957583E-4
34-35	0.0
36-37	0.0
38-39	9.222314052107542E-4
40-41	0.0
42-43	7.769188125020394E-5
44-45	0.0019523682010467915
46-47	0.0
48-49	0.0015495214093790676
50-51	0.0012056628979198315
52-53	6.387999125016769E-4
54-55	7.36634133335267E-4
56-57	0.008474170010438912
58-59	6.531872979183812E-4
60-61	0.0
62-63	0.0048442326698043825
64-65	1.4387385416704433E-6
66-67	0.002686124857298718
68-69	2.7767653854239555E-4
70-71	6.762071145851084E-5
72-73	1.5969997812541922E-4
74-75	5.668629854181546E-4
76-77	2.7623780000072515E-4
78-79	2.5753419895900933E-4
80-81	0.001694834002087782
82-83	0.005209672259388676
84-85	6.028314489599158E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	6.0427018750158614E-5
96-97	0.0047061137698040205
98-99	1.4387385416704433E-6
100-101	4.819774114595985E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.4752666E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.400367516610615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.100732754726174	20.120865643039465
2	15.185783530820421	9.840499348525485
3	7.128781567577347	6.929254282054366
4	3.9993239963916785	5.183182692043611
5	2.46739250639698	3.997221200749658
6	1.6912336463361048	3.2877955018648346
7	1.2588827098257624	2.8551783721062964
8	0.941699763398544	2.440913473953447
9	0.6913040979600126	2.0158656155679053
>10	4.083466477807575	24.09566328605375
>50	0.2883355001500546	6.415506420404543
>100	0.15023363892674155	8.919552297173771
>500	0.009505556411816997	2.1126829253435657
>1k	0.003268207722351368	1.6290072108434996
>5k	4.6704623548913396E-5	0.09825815210732888
>10k+	9.340924709782679E-6	0.05855357816856071
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.165224791674975E-5	5.754954166681773E-6	2.0142339583386206E-5	0.0	0.0
2	4.603963333345419E-5	1.4387385416704434E-5	3.165224791674975E-5	2.8774770833408867E-6	0.0
3	6.905945000018129E-5	1.4387385416704434E-5	3.165224791674975E-5	2.8774770833408867E-6	0.0
4	1.3236394583368078E-4	1.4387385416704434E-5	4.603963333345419E-5	2.8774770833408867E-6	0.0
5	1.496288083337261E-4	1.4387385416704434E-5	4.891711041679507E-5	5.754954166681773E-6	0.0
6	1.726486250004532E-4	1.4387385416704434E-5	6.905945000018129E-5	8.632431250022661E-6	0.0
7	1.8415853333381675E-4	1.4387385416704434E-5	8.920178958356749E-5	1.4387385416704434E-5	0.0
8	2.0717835000054385E-4	1.4387385416704434E-5	1.1222160625029458E-4	1.4387385416704434E-5	0.0
9	2.4746302916731625E-4	1.4387385416704434E-5	1.1797656041697636E-4	1.7264862500045322E-5	0.0
10-11	2.963801395841113E-4	1.4387385416704434E-5	1.496288083337261E-4	1.7264862500045322E-5	2.8774770833408867E-6
12-13	3.251549104175202E-4	1.5826123958374876E-5	1.726486250004532E-4	2.158107812505665E-5	2.8774770833408867E-6
14-15	3.8414319062600834E-4	2.0142339583386206E-5	1.9710718020885074E-4	3.165224791674975E-5	2.8774770833408867E-6
16-17	4.805386729179281E-4	2.158107812505665E-5	2.992576166674522E-4	3.4529725000090644E-5	5.754954166681773E-6
18-19	5.769341552098478E-4	2.4458555208397535E-5	3.654395895842926E-4	3.740720208343153E-5	5.754954166681773E-6
20-21	6.877170229184718E-4	2.589729375006798E-5	4.229891312511104E-4	3.740720208343153E-5	5.754954166681773E-6
22-23	8.574881708355843E-4	2.877477083340887E-5	4.7909993437625766E-4	4.028467916677241E-5	5.754954166681773E-6
24-25	0.0011466746177113433	3.3090986458420195E-5	4.992422739596438E-4	4.028467916677241E-5	5.754954166681773E-6
26-27	0.0014804619593788862	3.740720208343153E-5	5.121909208346779E-4	4.028467916677241E-5	7.193692708352217E-6
28-29	0.001884747489588281	4.31621562501133E-5	5.783728937515183E-4	4.028467916677241E-5	8.632431250022661E-6
30-31	0.00249908884688156	4.31621562501133E-5	5.884440635432113E-4	4.603963333345419E-5	8.632431250022661E-6
32-33	0.0032544265812585425	4.4600894791783745E-5	5.956377562515635E-4	4.603963333345419E-5	8.632431250022661E-6
34-35	0.004110476013552456	4.603963333345419E-5	7.510215187519714E-4	4.603963333345419E-5	1.0071169791693105E-5
36-37	0.005139174070846823	4.747837187512463E-5	8.157647531271415E-4	4.603963333345419E-5	1.4387385416704434E-5
38-39	0.006504536946892074	4.891711041679507E-5	8.50294478127232E-4	4.891711041679507E-5	1.5826123958374876E-5
40-41	0.008313031293771821	5.035584895846551E-5	9.049665427107089E-4	4.891711041679507E-5	1.7264862500045322E-5
42-43	0.010735866997944848	6.042701875015862E-5	9.855359010442536E-4	4.891711041679507E-5	1.8703601041715764E-5
44-45	0.013364442313576749	6.618197291684039E-5	0.0010114331947943217	5.179458750013596E-5	2.3019816666727093E-5
46-47	0.016303785154209466	6.618197291684039E-5	0.0011121448927112528	5.179458750013596E-5	2.3019816666727093E-5
48-49	0.020182624262552983	6.905945000018129E-5	0.0011625007416697182	5.179458750013596E-5	2.3019816666727093E-5
50-51	0.025032611886524045	6.905945000018129E-5	0.0011668169572947294	5.179458750013596E-5	2.3019816666727093E-5
52-53	0.031165954289665142	6.905945000018129E-5	0.0012315601916698996	5.3233326041806404E-5	2.3019816666727093E-5
54-55	0.03795680020634964	7.193692708352217E-5	0.0012775998250033537	5.4672064583476846E-5	2.589729375006798E-5
56-57	0.04560369555532805	8.056935833354482E-5	0.0013322718895868305	5.4672064583476846E-5	2.589729375006798E-5
58-59	0.05448646731160136	8.056935833354482E-5	0.00136248539896191	5.4672064583476846E-5	2.589729375006798E-5
60-61	0.06625103236684057	8.488557395855616E-5	0.001424351156253739	5.6110803125147295E-5	2.7336032291738426E-5
62-63	0.08051612500750302	8.920178958356749E-5	0.0015811736572958171	5.754954166681774E-5	2.877477083340887E-5
64-65	0.09778386498463168	9.351800520857882E-5	0.001674691662504396	6.618197291684039E-5	2.877477083340887E-5
66-67	0.11799094780239305	1.0358917500027192E-4	0.0016833240937544187	6.905945000018129E-5	2.877477083340887E-5
68-69	0.1418207742680806	1.0646665208361281E-4	0.001700588956254464	6.905945000018129E-5	2.877477083340887E-5
70-71	0.17120700898169944	1.0646665208361281E-4	0.0017077826489628163	6.905945000018129E-5	3.452972500009064E-5
72-73	0.20819122193387984	1.0646665208361281E-4	0.0017451898510462479	6.905945000018129E-5	4.028467916677241E-5
74-75	0.2553717749308787	1.1078286770862413E-4	0.0017523835437546	6.905945000018129E-5	4.028467916677241E-5
76-77	0.31345508859665616	1.1366034479196502E-4	0.0017595772364629523	6.905945000018129E-5	4.172341770844286E-5
78-79	0.3834971970207983	1.194152989586468E-4	0.0017696484062546453	7.193692708352217E-5	4.31621562501133E-5
80-81	0.4675166503772689	1.222927760419877E-4	0.0017754033604213272	7.193692708352217E-5	4.31621562501133E-5
82-83	0.5699404471587877	1.3668016145869212E-4	0.0018185655166714403	7.193692708352217E-5	4.31621562501133E-5
84-85	0.6974299468132891	1.3811890000036257E-4	0.0018444628104215084	7.481440416686306E-5	4.4600894791783745E-5
86-87	0.8566565799584986	1.4099637708370346E-4	0.0018602889343798832	7.481440416686306E-5	4.747837187512463E-5
88-89	1.0499755615871311	1.4387385416704434E-4	0.0018703601041715763	7.481440416686306E-5	4.891711041679507E-5
90	1.2142982066469374	1.5826123958374876E-4	0.0018703601041715763	7.481440416686306E-5	4.891711041679507E-5
>>END_MODULE
