##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654139_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	31477783
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66264174322569	32.0	32.0	32.0	32.0	32.0
2	31.69203263139593	32.0	32.0	32.0	32.0	32.0
3	35.8184638035023	37.0	37.0	37.0	32.0	37.0
4	36.379330177096655	37.0	37.0	37.0	37.0	37.0
5	36.5532364842848	37.0	37.0	37.0	37.0	37.0
6	40.157058900876216	41.0	41.0	41.0	37.0	41.0
7	40.18370648911329	41.0	41.0	41.0	37.0	41.0
8	40.230380297113044	41.0	41.0	41.0	41.0	41.0
9	40.27858791707154	41.0	41.0	41.0	41.0	41.0
10-11	40.31119016545733	41.0	41.0	41.0	41.0	41.0
12-13	40.29896109583067	41.0	41.0	41.0	41.0	41.0
14-15	40.28538266179673	41.0	41.0	41.0	41.0	41.0
16-17	40.27995276859237	41.0	41.0	41.0	41.0	41.0
18-19	40.27106060804854	41.0	41.0	41.0	41.0	41.0
20-21	40.23660062082517	41.0	41.0	41.0	41.0	41.0
22-23	40.2359466039905	41.0	41.0	41.0	41.0	41.0
24-25	40.21781147992538	41.0	41.0	41.0	41.0	41.0
26-27	40.12279368594669	41.0	41.0	41.0	41.0	41.0
28-29	40.11903968904036	41.0	41.0	41.0	37.0	41.0
30-31	40.01663431951354	41.0	41.0	41.0	37.0	41.0
32-33	40.02974022979954	41.0	41.0	41.0	37.0	41.0
34-35	40.02783526717876	41.0	41.0	41.0	37.0	41.0
36-37	39.99751718537484	41.0	41.0	41.0	37.0	41.0
38-39	39.900977381412154	41.0	41.0	41.0	37.0	41.0
40-41	39.89767895979205	41.0	41.0	41.0	37.0	41.0
42-43	39.84893361136646	41.0	41.0	41.0	37.0	41.0
44-45	39.83655645316571	41.0	41.0	41.0	37.0	41.0
46-47	39.79553907910224	41.0	41.0	41.0	37.0	41.0
48-49	39.678092736073566	41.0	41.0	41.0	37.0	41.0
50-51	39.59261848269301	41.0	41.0	41.0	37.0	41.0
52-53	39.599146610801654	41.0	41.0	41.0	37.0	41.0
54-55	39.53380746350529	41.0	41.0	41.0	37.0	41.0
56-57	39.4680685580684	41.0	41.0	41.0	37.0	41.0
58-59	39.41460828102157	41.0	41.0	41.0	37.0	41.0
60-61	39.331506589901835	41.0	41.0	41.0	37.0	41.0
62-63	39.196982249353454	41.0	41.0	41.0	37.0	41.0
64-65	39.02814407228108	41.0	41.0	41.0	37.0	41.0
66-67	38.89503223591064	41.0	41.0	41.0	32.0	41.0
68-69	38.78366341746495	41.0	41.0	41.0	32.0	41.0
70-71	38.65070605512466	41.0	41.0	41.0	32.0	41.0
72-73	38.49406924560094	41.0	37.0	41.0	32.0	41.0
74-75	38.36396308469374	41.0	37.0	41.0	32.0	41.0
76-77	36.62126594175962	39.0	34.5	41.0	27.0	41.0
78-79	37.35628169874606	41.0	37.0	41.0	29.5	41.0
80-81	38.06895866840432	41.0	37.0	41.0	32.0	41.0
82-83	38.20717886961734	41.0	37.0	41.0	32.0	41.0
84-85	38.23256471079936	41.0	37.0	41.0	32.0	41.0
86-87	38.21444823480739	41.0	37.0	41.0	32.0	41.0
88-89	38.069994748359505	41.0	37.0	41.0	32.0	41.0
90-91	38.02232650882688	41.0	37.0	41.0	32.0	41.0
92-93	37.9799080195705	41.0	37.0	41.0	32.0	41.0
94-95	37.97868771444291	41.0	37.0	41.0	32.0	41.0
96-97	37.94679194846727	41.0	37.0	41.0	32.0	41.0
98-99	37.920402462905344	41.0	37.0	41.0	32.0	41.0
100-101	37.000032705607	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	2.0
15	3.0
16	7.0
17	15.0
18	51.0
19	185.0
20	799.0
21	2522.0
22	6515.0
23	14156.0
24	27304.0
25	47956.0
26	76172.0
27	114479.0
28	159168.0
29	209907.0
30	264397.0
31	323094.0
32	391129.0
33	477330.0
34	569835.0
35	705003.0
36	937257.0
37	1387647.0
38	2575636.0
39	7219025.0
40	1.5968186E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.393072096229936	12.289587120142667	6.8471064956901655	44.470234287937224
2	23.711263274163876	13.884183012507584	32.264311625758396	30.140242087570147
3	20.36276906173173	16.272631409972632	22.00282821840571	41.361771309889924
4	23.495777958695502	23.594574624267533	21.483714402631215	31.42593301440575
5	24.3983987055251	28.227826591218317	24.02682234641493	23.346952356841648
6	20.25027302589893	31.322930207632478	25.524863043880824	22.90193372258777
7	16.04396027509307	25.454832698986458	39.5396238674115	18.961583158508972
8	18.613760060548103	24.478229613565862	31.814051199222003	25.093959126664032
9	18.487261316973942	23.0863177371799	34.10088315304798	24.325537792798176
10-11	21.393335497623088	30.736700808501688	24.905773497599515	22.96419019627571
12-13	21.60143076149931	25.09166703385686	27.48806197691877	25.818840227725058
14-15	21.144222577555734	25.899493938311984	27.449441086749975	25.506842397382307
16-17	21.671612097127095	26.33486315702728	26.848652831575897	25.14487191426972
18-19	21.422383946601514	26.554546809641078	26.75424882838814	25.26882041536927
20-21	21.68227667113659	26.54694105998507	26.842322408792256	24.92845986008608
22-23	21.623716320809507	26.788689343210738	26.743042862961474	24.844551473018285
24-25	21.584765898270927	26.408864331999364	26.620340712440445	25.386029057289267
26-27	21.375665179469596	26.572405051524754	26.827481782945135	25.224447986060518
28-29	21.540878977156186	26.675803190701146	26.77111037644615	25.012207455696515
30-31	21.53332683869268	26.485418327159003	26.730835368309265	25.25041946583905
32-33	21.679630360244875	26.35420194617899	26.799538264813634	25.166629428762505
34-35	21.785837013998094	26.460217989303757	26.560495064090123	25.193449932608026
36-37	21.57521862324294	26.437027029508396	26.487958189431577	25.499796157817084
38-39	21.643699883101604	26.488861294762494	26.760301551855093	25.107137270280806
40-41	21.86004684415111	26.649696496250968	26.608570664086646	24.88168599551128
42-43	21.803211172781765	26.276944281622377	26.57555012689426	25.344294418701597
44-45	21.762595224701816	26.260065710472684	26.74668193754306	25.23065712728244
46-47	21.852420936928418	26.43519691402326	26.581928980402754	25.130453168645566
48-49	21.730248601053002	26.326981160013712	26.5918632198462	25.35090701908708
50-51	21.794193446788015	26.26438032465113	26.699965692734036	25.24146053582682
52-53	22.141986558355008	26.392973382470252	26.423725389280932	25.041314669893804
54-55	21.990575066865414	26.21497358946785	26.417275003134748	25.37717634053199
56-57	21.844196711028847	26.1186364357801	26.85134720081897	25.185819652372082
58-59	21.858464750695447	26.296533506882458	26.670126149008595	25.1748755934135
60-61	21.788361840052207	26.130829982228853	26.607045752428416	25.473762425290523
62-63	21.891702146663793	26.094580500761932	26.882826792904936	25.13089055966934
64-65	22.139956400132668	26.292575314926758	26.506945035702078	25.060523249238496
66-67	21.874315433132004	26.176866496906133	26.5206249935569	25.428193076404966
68-69	21.86795204407698	26.198868689551364	26.717454016190217	25.215725250181436
70-71	22.023878393877226	26.474988764988726	26.532502873322763	24.96862996781129
72-73	21.987722261126205	26.316678655545722	26.445555266709857	25.250043816618216
74-75	21.94433610524604	26.252036237749017	26.69596203773309	25.107665619271852
76-77	21.972894625680777	26.408201392331716	26.539434877650404	25.079469104337104
78-79	21.89480538836576	26.19723693043616	26.562950441726652	25.345007239471435
80-81	21.93083598572632	26.218391602108902	26.726112483319692	25.124659928845087
82-83	22.06236514342359	26.458589065566134	26.407876527884767	25.07116926312551
84-85	22.09568214550873	26.22979906028617	26.401874440011635	25.272644354193464
86-87	22.083836967481098	26.20065705741052	26.639434484275853	25.07607149083253
88-89	22.2469956032164	26.38037596230967	26.514410179395416	24.85821825507851
90-91	22.17626383130566	26.208562631984655	26.454914397570505	25.16025913913918
92-93	22.12181992098686	26.19334623707981	26.551309166158987	25.13352467577435
94-95	22.31372203055088	26.33182902366409	26.352197357736408	25.002251588048622
96-97	22.24160966771986	26.32548154762825	26.187760246725766	25.245148537926127
98-99	22.274681689327565	26.393436769158747	26.416871024770007	24.91501051674368
100-101	22.27667685491065	26.64559159074195	26.261496878608	24.816234675739395
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	40.5
2	31.0
3	34.0
4	41.5
5	57.5
6	77.5
7	109.0
8	124.5
9	140.0
10	193.5
11	227.0
12	254.0
13	311.0
14	388.5
15	472.5
16	577.0
17	709.5
18	855.5
19	1075.0
20	1429.0
21	1873.0
22	2573.5
23	3690.5
24	5437.0
25	7756.0
26	10996.0
27	16001.0
28	23432.5
29	33870.0
30	48061.5
31	66885.0
32	93517.0
33	130015.0
34	177543.0
35	242763.5
36	324112.5
37	423398.5
38	559916.5
39	739002.5
40	952803.0
41	1185713.0
42	1423030.0
43	1643940.0
44	1827933.0
45	1957521.5
46	2029921.0
47	2037661.5
48	1969708.5
49	1846268.5
50	1687857.5
51	1516665.0
52	1356053.0
53	1203052.0
54	1037734.0
55	868535.0
56	715566.0
57	590628.5
58	487374.5
59	397617.0
60	325584.5
61	273851.0
62	229965.5
63	186205.5
64	158428.5
65	139442.5
66	118108.0
67	96887.5
68	68794.5
69	47539.0
70	40851.5
71	34842.5
72	28698.0
73	26343.5
74	21775.0
75	14666.5
76	8840.0
77	3009.5
78	246.5
79	52.0
80	20.0
81	7.5
82	3.5
83	5.0
84	3.0
85	0.0
86	0.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.5
97	0.5
98	0.0
99	0.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0597278404263731
2	0.0
3	5.082950092133235E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.527708225194894E-4
12-13	0.0
14-15	0.0
16-17	0.0012977406953977666
18-19	0.002017295817815378
20-21	0.0
22-23	0.0
24-25	1.1436637707299781E-4
26-27	0.0
28-29	2.9703489600903596E-4
30-31	0.0017393219846518415
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.002779738331635363
40-41	1.588421903791636E-6
42-43	0.0
44-45	0.0
46-47	0.0021681958986755835
48-49	0.0
50-51	0.0015439460904854703
52-53	5.162371187322817E-4
54-55	0.0
56-57	6.957287938607366E-4
58-59	1.540769246677887E-4
60-61	0.0011118953326541452
62-63	0.0032070238237553133
64-65	0.001979173692124379
66-67	9.737026270242729E-4
68-69	4.066360073706588E-4
70-71	9.181078603915658E-4
72-73	0.0
74-75	0.0
76-77	9.482878765636068E-4
78-79	4.765265711374908E-6
80-81	0.00173773356274805
82-83	0.001143663770729978
84-85	2.7003172364457816E-5
86-87	0.0010181784403304388
88-89	0.0
90-91	0.002030003193045711
92-93	0.0011484290364413528
94-95	0.0
96-97	2.589127703180367E-4
98-99	0.0011404869269223948
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.1477783E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.633773027880096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.73445371770388	22.073646041148848
2	15.755476688764777	10.913432071694704
3	6.683056696354635	6.943784062620018
4	3.545112851415433	4.911225354165716
5	2.1586435729977005	3.7380985777647244
6	1.511316417893548	3.1405553874380354
7	1.0962009228415421	2.6575901768252668
8	0.7978506415493649	2.2106062423655386
9	0.6204481548680794	1.9339614514141252
>10	3.6943984138014483	23.43003140297084
>50	0.2593506106479431	6.18388864556539
>100	0.13362989573888065	8.416619579864063
>500	0.007224208370983157	1.6960261384803346
>1k	0.0027889868595330058	1.5908850360959483
>5k	3.857615381282951E-5	0.07032902744647365
>10k+	9.644038453207377E-6	0.0893208041400835
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.812212569099927E-5	0.0	0.0	3.1768438075832724E-6	3.1768438075832724E-6
2	5.082950092133236E-5	0.0	1.270737523033309E-5	3.1768438075832724E-6	9.530531422749817E-6
3	7.624425138199853E-5	0.0	1.270737523033309E-5	3.1768438075832724E-6	2.8591594268249452E-5
4	1.2072006468816435E-4	0.0	2.2237906653082906E-5	3.1768438075832724E-6	2.8591594268249452E-5
5	1.3978112753366398E-4	0.0	2.8591594268249452E-5	3.1768438075832724E-6	3.176843807583272E-5
6	1.6837272180191343E-4	0.0	4.447581330616581E-5	3.1768438075832724E-6	3.4945281883415994E-5
7	1.8425694083982978E-4	0.0	4.447581330616581E-5	3.1768438075832724E-6	3.4945281883415994E-5
8	2.1284853510807923E-4	0.0	4.447581330616581E-5	3.1768438075832724E-6	3.4945281883415994E-5
9	2.4461697318391195E-4	0.0	4.447581330616581E-5	3.1768438075832724E-6	4.129896949858254E-5
10-11	2.811506769711196E-4	1.270737523033309E-5	5.2417922825124E-5	3.1768438075832724E-6	6.671371995924872E-5
12-13	3.2403806837349373E-4	2.8591594268249452E-5	5.7183188536498904E-5	3.1768438075832724E-6	9.371689232370652E-5
14-15	4.2728549211995016E-4	3.018001617204109E-5	5.7183188536498904E-5	3.1768438075832724E-6	1.0324742374645635E-4
16-17	5.19413962539865E-4	3.653370378720763E-5	5.7183188536498904E-5	3.1768438075832724E-6	1.0801268945783126E-4
18-19	5.988350577294468E-4	4.447581330616581E-5	5.7183188536498904E-5	3.1768438075832724E-6	1.0801268945783126E-4
20-21	6.861982624379868E-4	4.7652657113749086E-5	5.8771610440290544E-5	3.1768438075832724E-6	1.1118953326541453E-4
22-23	7.862688423768599E-4	4.7652657113749086E-5	6.036003234408218E-5	3.1768438075832724E-6	1.1754322088058107E-4
24-25	8.863394223157329E-4	6.353687615166546E-5	6.036003234408218E-5	3.1768438075832724E-6	1.1754322088058107E-4
26-27	0.0010102363308114805	8.259793899716508E-5	6.036003234408218E-5	3.1768438075832724E-6	1.2072006468816435E-4
28-29	0.0012103774906892268	8.577478280474835E-5	6.671371995924872E-5	3.1768438075832724E-6	1.2548533039953927E-4
30-31	0.0015915987475992196	8.577478280474835E-5	7.942109518958181E-5	3.1768438075832724E-6	1.2707375230333089E-4
32-33	0.0020442989901798356	8.895162661233163E-5	8.418636090095671E-5	3.1768438075832724E-6	1.2707375230333089E-4
34-35	0.0025923045469879503	9.371689232370652E-5	8.577478280474835E-5	3.1768438075832724E-6	1.3025059611091415E-4
36-37	0.0034516407969392253	9.848215803508144E-5	8.736320470853999E-5	4.765265711374908E-6	1.366042837260807E-4
38-39	0.00466042986572466	1.0165900184266472E-4	8.895162661233163E-5	6.353687615166545E-6	1.4136954943745562E-4
40-41	0.006285385473303504	1.1277795516920617E-4	8.895162661233163E-5	6.353687615166545E-6	1.4295797134124724E-4
42-43	0.008297916025407507	1.318390180147058E-4	8.895162661233163E-5	6.353687615166545E-6	1.445463932450389E-4
44-45	0.010774265773418668	1.8108009703224652E-4	9.212847041991489E-5	6.353687615166545E-6	1.5566534657158033E-4
46-47	0.013631836778339821	2.207906446270374E-4	9.212847041991489E-5	6.353687615166545E-6	1.6043061228295527E-4
48-49	0.017215316593293752	2.2237906653082906E-4	9.212847041991489E-5	6.353687615166545E-6	1.667842998981218E-4
50-51	0.02180426747334779	2.3349801985737053E-4	9.212847041991489E-5	6.353687615166545E-6	1.8266851893603816E-4
52-53	0.027654425345012383	2.4144012937632868E-4	9.212847041991489E-5	6.353687615166545E-6	1.9061062845499634E-4
54-55	0.034557706938890834	2.4461697318391195E-4	9.371689232370652E-5	6.353687615166545E-6	1.9061062845499634E-4
56-57	0.042051881480979776	2.811506769711196E-4	1.2389690849574762E-4	6.353687615166545E-6	1.937874722625796E-4
58-59	0.051004227330749434	2.92269630297661E-4	1.7472640941707998E-4	6.353687615166545E-6	1.937874722625796E-4
60-61	0.0627077199178862	3.097422712393691E-4	1.7472640941707998E-4	6.353687615166545E-6	1.937874722625796E-4
62-63	0.07717506661762044	3.27214912181077E-4	1.7472640941707998E-4	6.353687615166545E-6	1.937874722625796E-4
64-65	0.09410129042442411	3.303917559886603E-4	1.7472640941707998E-4	6.353687615166545E-6	2.0014115987774617E-4
66-67	0.11413287905314043	3.4468755312278503E-4	1.7472640941707998E-4	6.353687615166545E-6	2.0014115987774617E-4
68-69	0.1374413820693789	3.4945281883415997E-4	1.7631483132087163E-4	6.353687615166545E-6	2.0172958178153781E-4
70-71	0.1664427891888066	3.4945281883415997E-4	1.7790325322466325E-4	6.353687615166545E-6	2.1126011320428758E-4
72-73	0.20278429392565545	3.4945281883415997E-4	1.8425694083982978E-4	6.353687615166545E-6	2.1284853510807923E-4
74-75	0.2467391048473776	3.5739492835311815E-4	1.890222065512047E-4	6.353687615166545E-6	2.1284853510807923E-4
76-77	0.30026415773944437	3.986938978517007E-4	1.9061062845499634E-4	6.353687615166545E-6	2.192022227232458E-4
78-79	0.36460636379633216	4.19343382600992E-4	1.92199050358788E-4	6.353687615166545E-6	2.239674884346207E-4
80-81	0.4406393550651264	4.225202264085752E-4	1.9537589416637123E-4	6.353687615166545E-6	2.3032117604978724E-4
82-83	0.5341910515108386	4.225202264085752E-4	2.0331800368532943E-4	6.353687615166545E-6	2.414401293763287E-4
84-85	0.6498726419201759	4.384044454464916E-4	2.0331800368532943E-4	6.353687615166545E-6	2.4302855128012033E-4
86-87	0.7931657702831232	4.527002425806163E-4	2.0967169130049597E-4	6.353687615166545E-6	2.493822388952869E-4
88-89	0.963883002815033	4.6699603971474106E-4	2.1284853510807923E-4	6.353687615166545E-6	2.5097066079907853E-4
90	1.110205251748511	4.733497273299076E-4	2.1284853510807923E-4	6.353687615166545E-6	2.5097066079907853E-4
>>END_MODULE
