##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654112_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35332753
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.68093881617433	32.0	32.0	32.0	32.0	32.0
2	31.708007043776067	32.0	32.0	32.0	32.0	32.0
3	35.85097900523064	37.0	37.0	37.0	32.0	37.0
4	36.40204517887412	37.0	37.0	37.0	37.0	37.0
5	36.572499204916184	37.0	37.0	37.0	37.0	37.0
6	40.19827277540474	41.0	41.0	41.0	37.0	41.0
7	40.23084680098378	41.0	41.0	41.0	37.0	41.0
8	40.26442185243816	41.0	41.0	41.0	41.0	41.0
9	40.30969347336167	41.0	41.0	41.0	41.0	41.0
10-11	40.339506307363024	41.0	41.0	41.0	41.0	41.0
12-13	40.31940766121451	41.0	41.0	41.0	41.0	41.0
14-15	40.31422657611763	41.0	41.0	41.0	41.0	41.0
16-17	40.31234555937377	41.0	41.0	41.0	41.0	41.0
18-19	40.31144363418271	41.0	41.0	41.0	41.0	41.0
20-21	40.280955067384646	41.0	41.0	41.0	41.0	41.0
22-23	40.2868628861159	41.0	41.0	41.0	41.0	41.0
24-25	40.27515389191439	41.0	41.0	41.0	41.0	41.0
26-27	40.196151033008945	41.0	41.0	41.0	41.0	41.0
28-29	40.20722731398824	41.0	41.0	41.0	41.0	41.0
30-31	40.11811844947378	41.0	41.0	41.0	41.0	41.0
32-33	40.143958581432926	41.0	41.0	41.0	41.0	41.0
34-35	40.160798296696555	41.0	41.0	41.0	41.0	41.0
36-37	40.146888610689345	41.0	41.0	41.0	41.0	41.0
38-39	40.07534163839427	41.0	41.0	41.0	37.0	41.0
40-41	40.09583605047702	41.0	41.0	41.0	37.0	41.0
42-43	40.083892472233906	41.0	41.0	41.0	37.0	41.0
44-45	40.07939374268402	41.0	41.0	41.0	37.0	41.0
46-47	40.068442076959016	41.0	41.0	41.0	37.0	41.0
48-49	39.97676509950979	41.0	41.0	41.0	37.0	41.0
50-51	39.93831114716705	41.0	41.0	41.0	37.0	41.0
52-53	39.982781585120186	41.0	41.0	41.0	37.0	41.0
54-55	39.95132111556663	41.0	41.0	41.0	37.0	41.0
56-57	39.94239187928549	41.0	41.0	41.0	37.0	41.0
58-59	39.94088840176139	41.0	41.0	41.0	37.0	41.0
60-61	39.92428053936244	41.0	41.0	41.0	37.0	41.0
62-63	39.87926588964069	41.0	41.0	41.0	37.0	41.0
64-65	39.82575153427756	41.0	41.0	41.0	37.0	41.0
66-67	39.80206214047345	41.0	41.0	41.0	37.0	41.0
68-69	39.77980435037145	41.0	41.0	41.0	37.0	41.0
70-71	39.7582350857291	41.0	41.0	41.0	37.0	41.0
72-73	39.735207570720576	41.0	41.0	41.0	37.0	41.0
74-75	39.713351589104875	41.0	41.0	41.0	37.0	41.0
76-77	39.180694552728454	41.0	39.0	41.0	34.5	41.0
78-79	39.50948772658615	41.0	41.0	41.0	37.0	41.0
80-81	39.690831450354295	41.0	41.0	41.0	37.0	41.0
82-83	39.76168568013933	41.0	41.0	41.0	37.0	41.0
84-85	39.79977441893645	41.0	41.0	41.0	37.0	41.0
86-87	39.806387588875396	41.0	41.0	41.0	37.0	41.0
88-89	39.68889797520165	41.0	41.0	41.0	37.0	41.0
90-91	39.71152195528042	41.0	41.0	41.0	37.0	41.0
92-93	39.664409464498846	41.0	41.0	41.0	37.0	41.0
94-95	39.65861113341494	41.0	41.0	41.0	37.0	41.0
96-97	39.62483974854719	41.0	41.0	41.0	37.0	41.0
98-99	39.583578415188875	41.0	41.0	41.0	37.0	41.0
100-101	39.061327177081274	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	2.0
17	20.0
18	69.0
19	168.0
20	484.0
21	1503.0
22	3744.0
23	8021.0
24	15373.0
25	27344.0
26	45312.0
27	71681.0
28	104119.0
29	144288.0
30	190159.0
31	239894.0
32	296415.0
33	361800.0
34	431876.0
35	530023.0
36	672322.0
37	912668.0
38	1420007.0
39	3391074.0
40	2.6464385E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.516114775733435	10.42598692901968	9.09186087956713	44.96603741567975
2	22.58696909352068	12.964625202004498	34.941322007939775	29.50708369653505
3	20.165108130355307	16.555997777018266	23.72979790632616	39.54909618630027
4	23.252880974205432	24.666263622311003	22.40522271219568	29.675632691287884
5	24.192736976934686	29.689820660167637	24.586954772530746	21.53048759036693
6	20.420998612816838	32.51413214249113	24.689913067345756	22.374956177346274
7	16.395832501362122	23.704102536250147	40.47520723901701	19.42485772337072
8	19.62068452463922	23.894885858455467	30.394280343793195	26.090149273112118
9	19.225167651102648	22.35980026804025	33.30443002842151	25.1106020524356
10-11	22.10658720799029	30.290737759466445	23.913835671126137	23.68883936141713
12-13	22.33736074853833	23.78132267247899	27.069696493788637	26.811620085194043
14-15	21.186370617653257	25.534333540327296	27.70939756661475	25.569898275404697
16-17	21.94889292350722	25.915126963468904	26.83476361460449	25.301216498419382
18-19	21.761391155302313	26.328297045128053	26.59593206710419	25.314379732465454
20-21	21.985193171899173	26.118106336067274	26.734438157139923	25.162262334893633
22-23	21.963250358668628	26.397300544341963	26.67057390065246	24.968875196336953
24-25	21.887835705348934	26.113548394364095	26.51047515752009	25.48814074276688
26-27	21.703494205503883	26.27529193663454	26.71094862039196	25.310265237469608
28-29	21.83692534259895	26.413348292495222	26.60019536044279	25.14953100446304
30-31	21.92104375974883	26.158227066816874	26.556230407432015	25.36449876600228
32-33	21.972103334263256	26.095310206934624	26.69411862698613	25.238467831815992
34-35	22.050534811142512	26.27219707448214	26.45190285625352	25.225365258121833
36-37	21.899311410850313	26.092836950645154	26.389514940847025	25.618336697657508
38-39	22.03210657092046	26.1916512771753	26.579812182754264	25.196429969149975
40-41	22.17115518850173	26.415807452082774	26.4315294084217	24.981507950993798
42-43	22.12240438779282	26.021131158390066	26.450167922097663	25.406296531719452
44-45	22.10680130133081	26.038300780015643	26.65562318339587	25.19927473525768
46-47	22.08936863268598	26.1966470838775	26.48254545625907	25.23143882717745
48-49	22.03346424774769	26.044978719886338	26.407120045245268	25.514436987120703
50-51	22.07169884491487	26.04725785410312	26.56810001731848	25.312943283663536
52-53	22.452323502034517	26.179341014185447	26.27115853394409	25.097176949835948
54-55	22.212465300963103	26.04450607061386	26.284422275275293	25.458606353147744
56-57	22.148571456712244	25.991492227123892	26.671854239863237	25.188082076300628
58-59	22.25296899888859	26.049252504075476	26.527872862026598	25.169905635009336
60-61	22.192141677032872	25.88243148939907	26.467161739731488	25.45826509383657
62-63	22.21750185892608	25.906668615255278	26.59239132565222	25.28343820016642
64-65	22.35728637731852	26.12863641859553	26.387987597424388	25.126089606661562
66-67	22.223309043306635	25.919845105353367	26.42859058338872	25.428255267951283
68-69	22.275175674501313	25.903394013973525	26.615324285401158	25.206106026124004
70-71	22.488996442560698	26.158683874537157	26.37886823374227	24.973451449159874
72-73	22.399726395506175	25.990423106854994	26.275157500464232	25.334692997174606
74-75	22.328820513929383	25.982403635516317	26.510559480038253	25.178216370516047
76-77	22.32148018582886	26.141502156313944	26.384923198611336	25.152094459245856
78-79	22.338242999016888	25.94763351578162	26.356528922513593	25.3575945626879
80-81	22.45255571508616	26.022442690755643	26.46127559770177	25.063725996456427
82-83	22.55493120762008	26.23544289990054	26.17129909551108	25.038326796968295
84-85	22.58190397706694	26.07386003697929	26.116885279256664	25.227350706697106
86-87	22.57731115372831	26.053963066360435	26.306360517372035	25.062365262539217
88-89	22.77554341718009	26.184404594796224	26.175763886838933	24.864288101184755
90-91	22.68743800771057	26.003959864728916	26.124652103764817	25.1839500237957
92-93	22.677900566598435	26.08078278421534	26.08507913111054	25.156237518075685
94-95	22.885338145035007	26.26478468858625	25.887743590203684	24.96213357617506
96-97	22.837719870252606	26.312573274989575	25.65342950725244	25.19627734750538
98-99	22.9180401432654	26.416584075872162	25.85114297947114	24.814232801391302
100-101	22.9902280753498	26.5820512202941	25.622508101760427	24.805212602595674
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	36.0
2	28.0
3	22.5
4	30.5
5	34.0
6	39.5
7	51.5
8	68.5
9	89.0
10	100.0
11	114.5
12	148.5
13	180.5
14	221.0
15	258.5
16	316.0
17	385.0
18	475.5
19	624.5
20	837.5
21	1142.0
22	1669.5
23	2515.5
24	4057.0
25	6020.0
26	8795.5
27	13170.5
28	19395.5
29	28468.0
30	41547.5
31	58370.0
32	81767.5
33	114633.0
34	157327.5
35	218265.5
36	301103.5
37	410090.5
38	560270.0
39	762710.0
40	1000523.5
41	1256947.0
42	1530181.5
43	1797267.0
44	2024411.0
45	2176139.5
46	2260749.5
47	2279027.0
48	2213387.0
49	2089843.0
50	1932769.5
51	1752334.5
52	1590399.0
53	1436746.5
54	1252328.0
55	1062725.0
56	891828.5
57	733854.0
58	591029.5
59	477891.5
60	394111.0
61	338770.0
62	290030.5
63	237528.5
64	201471.5
65	176394.0
66	149878.5
67	121489.0
68	84810.5
69	55851.5
70	41960.0
71	31344.5
72	24010.5
73	18742.5
74	11715.5
75	5322.0
76	2382.5
77	900.0
78	138.5
79	50.5
80	22.0
81	11.5
82	5.5
83	5.5
84	7.0
85	3.0
86	1.5
87	1.0
88	0.5
89	0.5
90	1.0
91	1.0
92	1.0
93	1.5
94	1.5
95	1.5
96	2.0
97	1.5
98	0.0
99	0.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.060134006540616854
2	0.0
3	8.207681977116247E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	4.910458010447134E-4
12-13	0.0
14-15	0.0
16-17	0.001368418702046795
18-19	0.0019259750294577953
20-21	0.0
22-23	0.0
24-25	1.0896405383412948E-4
26-27	0.0
28-29	3.226468087556042E-4
30-31	0.001804274917383313
32-33	0.0
34-35	0.0
36-37	1.4151175822614217E-6
38-39	0.002868443339243902
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0022429613678843535
48-49	0.0
50-51	0.0015622898108166097
52-53	4.853853307156677E-4
54-55	0.0
56-57	6.778413219032211E-4
58-59	1.4717222855518787E-4
60-61	0.0011108673020752162
62-63	0.0033892066095161056
64-65	0.0020858833162533358
66-67	9.919974251652568E-4
68-69	4.2312015709616515E-4
70-71	8.943543119892187E-4
72-73	0.0
74-75	0.0
76-77	9.948276603297796E-4
78-79	2.8302351645228435E-6
80-81	0.0017717272129913002
82-83	0.0012000197097576859
84-85	1.6981410987137063E-5
86-87	0.0010853951855945105
88-89	0.0
90-91	0.0020887135514178587
92-93	0.0011618115350366274
94-95	0.0
96-97	2.7453281095871584E-4
98-99	0.0012042650625044699
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.5332753E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.410349922990722
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.111139262984835	19.509326183659617
2	15.572790735116786	9.782936125350524
3	6.79331111414897	6.4014083769348336
4	3.798093252382926	4.7719775238999045
5	2.423832711485175	3.8066716811270385
6	1.692516819292579	3.1897527326716295
7	1.2111758219374693	2.663041946972527
8	0.901468066174458	2.2652341922353187
9	0.7149996935705406	2.0212551512893637
>10	4.293231449196969	24.770946537709413
>50	0.30857087935809874	6.647592220325999
>100	0.16436038091982882	9.525928025310483
>500	0.009965199817393989	2.1127250041414714
>1k	0.0045066207528045715	2.462692980436636
>5k	3.7992861156271744E-5	0.06851131793519612
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.50954087840254E-5	2.830235164522844E-6	0.0	2.830235164522844E-6	2.830235164522844E-6
2	1.0471870108734521E-4	2.830235164522844E-6	0.0	2.830235164522844E-6	5.660470329045688E-6
3	1.4434199339066503E-4	2.830235164522844E-6	0.0	2.830235164522844E-6	1.4151175822614218E-5
4	2.264188131618275E-4	2.830235164522844E-6	2.830235164522844E-6	2.830235164522844E-6	1.4151175822614218E-5
5	2.603816351361016E-4	2.830235164522844E-6	8.490705493568531E-6	5.660470329045688E-6	1.4151175822614218E-5
6	2.9434445711037574E-4	2.830235164522844E-6	1.6981410987137063E-5	5.660470329045688E-6	1.4151175822614218E-5
7	3.3396774941369555E-4	2.830235164522844E-6	1.6981410987137063E-5	5.660470329045688E-6	1.9811646151659905E-5
8	4.075538636912895E-4	8.490705493568531E-6	1.6981410987137063E-5	5.660470329045688E-6	1.9811646151659905E-5
9	4.443469208300865E-4	1.4151175822614218E-5	1.6981410987137063E-5	1.4151175822614218E-5	2.264188131618275E-5
10-11	5.136876823608962E-4	3.113258680975128E-5	1.6981410987137063E-5	1.4151175822614218E-5	2.5472116480705594E-5
12-13	5.759528559803987E-4	4.245352746784265E-5	1.6981410987137063E-5	1.4151175822614218E-5	2.9717469227489858E-5
14-15	7.174646142065409E-4	4.386864505010408E-5	1.6981410987137063E-5	1.4151175822614218E-5	3.113258680975128E-5
16-17	7.924658460663962E-4	4.52837626323655E-5	1.9811646151659905E-5	1.4151175822614218E-5	3.113258680975128E-5
18-19	8.886938416601728E-4	4.52837626323655E-5	1.9811646151659905E-5	1.4151175822614218E-5	3.113258680975128E-5
20-21	9.707706614313354E-4	4.52837626323655E-5	1.9811646151659905E-5	1.4151175822614218E-5	3.3962821974274125E-5
22-23	0.0010655835394428508	4.52837626323655E-5	1.9811646151659905E-5	1.6981410987137063E-5	3.3962821974274125E-5
24-25	0.0012000197097576857	6.085005603724113E-5	1.9811646151659905E-5	1.6981410987137063E-5	3.3962821974274125E-5
26-27	0.0014108722295146376	8.490705493568532E-5	2.264188131618275E-5	1.6981410987137063E-5	3.3962821974274125E-5
28-29	0.001723613215194412	8.773729010020816E-5	2.8302351645228436E-5	1.6981410987137063E-5	3.679305713879697E-5
30-31	0.002114185667898564	8.773729010020816E-5	3.113258680975128E-5	1.6981410987137063E-5	4.245352746784266E-5
32-33	0.002636364055753029	9.339776042925384E-5	3.3962821974274125E-5	1.8396528569398484E-5	4.52837626323655E-5
34-35	0.003292978613922329	1.0047334834056094E-4	3.3962821974274125E-5	1.9811646151659905E-5	4.669888021462693E-5
36-37	0.004333090036884474	1.0188846592282238E-4	3.3962821974274125E-5	1.9811646151659905E-5	4.952911537914976E-5
38-39	0.005970381079560938	1.0188846592282238E-4	3.3962821974274125E-5	1.9811646151659905E-5	5.235935054367261E-5
40-41	0.008477969435328178	1.0188846592282238E-4	3.3962821974274125E-5	1.9811646151659905E-5	5.377446812593403E-5
42-43	0.012148784443714308	1.2736058240352797E-4	3.3962821974274125E-5	1.9811646151659905E-5	5.660470329045687E-5
44-45	0.01619177537623519	1.485873461374493E-4	3.3962821974274125E-5	1.9811646151659905E-5	5.9434938454979715E-5
46-47	0.020698924875737816	1.7830481536493915E-4	3.3962821974274125E-5	1.9811646151659905E-5	6.226517361950256E-5
48-49	0.026799496772866804	1.81135050529462E-4	3.3962821974274125E-5	1.9811646151659905E-5	6.226517361950256E-5
50-51	0.03556756531255858	1.882106384407691E-4	4.103840988558123E-5	1.9811646151659905E-5	6.226517361950256E-5
52-53	0.047463043709048094	1.938711087698148E-4	4.245352746784265E-5	1.9811646151659905E-5	6.50954087840254E-5
54-55	0.06118968425698389	1.9670134393433763E-4	4.245352746784265E-5	1.9811646151659905E-5	7.217099669533252E-5
56-57	0.07615030733665162	2.504758120602717E-4	5.094423296141119E-5	1.9811646151659905E-5	7.358611427759394E-5
58-59	0.0942906996236608	2.589665175538402E-4	6.50954087840254E-5	1.9811646151659905E-5	7.500123185985537E-5
60-61	0.11726796380683951	2.71702575794193E-4	6.50954087840254E-5	1.9811646151659905E-5	7.924658460663962E-5
62-63	0.14661608734535914	2.7594792854097725E-4	6.50954087840254E-5	1.9811646151659905E-5	8.207681977116247E-5
64-65	0.18275677527873357	2.801932812877615E-4	6.50954087840254E-5	1.9811646151659905E-5	8.207681977116247E-5
66-67	0.22580040677837926	2.830235164522844E-4	6.50954087840254E-5	1.9811646151659905E-5	8.349193735342389E-5
68-69	0.27605972282997593	2.844386340345458E-4	6.792564394854825E-5	1.9811646151659905E-5	8.49070549356853E-5
70-71	0.3366465669969164	2.8585375161680723E-4	6.792564394854825E-5	2.122676373392133E-5	8.773729010020816E-5
72-73	0.4128209313324665	2.8868398678133007E-4	6.792564394854825E-5	2.264188131618275E-5	8.915240768246957E-5
74-75	0.5083583495460996	3.0566539776846714E-4	7.358611427759394E-5	2.264188131618275E-5	9.0567525264731E-5
76-77	0.6269140703527971	3.4528869007178695E-4	7.358611427759394E-5	2.264188131618275E-5	9.622799559377669E-5
78-79	0.7706235061841912	3.4670380765404837E-4	7.358611427759394E-5	2.264188131618275E-5	9.622799559377669E-5
80-81	0.9417126369971793	3.481189252363098E-4	7.358611427759394E-5	2.264188131618275E-5	1.0188846592282236E-4
82-83	1.1455546642516081	3.509491604008326E-4	7.358611427759394E-5	2.264188131618275E-5	1.1037917141639091E-4
84-85	1.3964238789997485	3.693456889702311E-4	7.500123185985537E-5	2.264188131618275E-5	1.1320940658091375E-4
86-87	1.7069332242522965	3.8491198237510676E-4	7.641634944211678E-5	2.264188131618275E-5	1.1320940658091375E-4
88-89	2.0804959636176665	4.174596867671194E-4	7.641634944211678E-5	2.264188131618275E-5	1.160396417454366E-4
90	2.3995045050692765	4.386864505010408E-4	7.924658460663962E-5	2.264188131618275E-5	1.160396417454366E-4
>>END_MODULE
