##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654129_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27646524
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29114973007095	32.0	32.0	32.0	32.0	32.0
2	31.350466626473548	32.0	32.0	32.0	32.0	32.0
3	34.699145469426824	37.0	32.0	37.0	32.0	37.0
4	35.67361064993198	37.0	37.0	37.0	32.0	37.0
5	35.81885798735494	37.0	37.0	37.0	32.0	37.0
6	39.04635374776229	41.0	41.0	41.0	37.0	41.0
7	39.43230946501629	41.0	41.0	41.0	37.0	41.0
8	39.51354687482593	41.0	41.0	41.0	37.0	41.0
9	39.35241399605969	41.0	41.0	41.0	37.0	41.0
10-11	39.393227372815474	41.0	41.0	41.0	37.0	41.0
12-13	39.468354502721574	41.0	41.0	41.0	37.0	41.0
14-15	39.49579726550795	41.0	41.0	41.0	37.0	41.0
16-17	39.286518496864204	41.0	41.0	41.0	37.0	41.0
18-19	39.365306303967905	41.0	41.0	41.0	37.0	41.0
20-21	39.36527320758299	41.0	41.0	41.0	37.0	41.0
22-23	39.2029802010553	41.0	41.0	41.0	37.0	41.0
24-25	39.19366092822374	41.0	41.0	41.0	37.0	41.0
26-27	39.1393550776944	41.0	41.0	41.0	37.0	41.0
28-29	39.01605143923337	41.0	41.0	41.0	37.0	41.0
30-31	39.16490796456003	41.0	41.0	41.0	37.0	41.0
32-33	39.268312645741645	41.0	41.0	41.0	37.0	41.0
34-35	39.15690804384667	41.0	41.0	41.0	37.0	41.0
36-37	38.93478100538064	41.0	41.0	41.0	37.0	41.0
38-39	38.94937057910065	41.0	41.0	41.0	37.0	41.0
40-41	38.90404216819445	41.0	41.0	41.0	37.0	41.0
42-43	38.7820757864533	41.0	41.0	41.0	32.0	41.0
44-45	38.80233041231513	41.0	41.0	41.0	32.0	41.0
46-47	38.77292302641735	41.0	41.0	41.0	32.0	41.0
48-49	38.80388541792813	41.0	41.0	41.0	32.0	41.0
50-51	38.70884918480168	41.0	41.0	41.0	32.0	41.0
52-53	38.78702856098654	41.0	41.0	41.0	32.0	41.0
54-55	38.66182828264414	41.0	41.0	41.0	32.0	41.0
56-57	38.64314184669291	41.0	41.0	41.0	32.0	41.0
58-59	38.62929576607895	41.0	41.0	41.0	32.0	41.0
60-61	38.64802508264692	41.0	41.0	41.0	32.0	41.0
62-63	38.658136444205425	41.0	41.0	41.0	32.0	41.0
64-65	38.649999508075595	41.0	41.0	41.0	32.0	41.0
66-67	38.5983312947407	41.0	41.0	41.0	32.0	41.0
68-69	38.551379750307845	41.0	41.0	41.0	32.0	41.0
70-71	38.5020424810005	41.0	41.0	41.0	32.0	41.0
72-73	38.456881469077274	41.0	41.0	41.0	32.0	41.0
74-75	38.428607589149365	41.0	41.0	41.0	32.0	41.0
76-77	37.60252449819731	41.0	39.0	41.0	29.5	41.0
78-79	38.16296453760336	41.0	37.0	41.0	32.0	41.0
80-81	38.59063884486889	41.0	41.0	41.0	32.0	41.0
82-83	38.62998451450895	41.0	41.0	41.0	32.0	41.0
84-85	38.505095939004846	41.0	41.0	41.0	32.0	41.0
86-87	38.542620023406926	41.0	41.0	41.0	32.0	41.0
88-89	38.42098912687902	41.0	41.0	41.0	32.0	41.0
90-91	38.321134837782864	41.0	39.0	41.0	32.0	41.0
92-93	38.2983459692799	41.0	37.0	41.0	32.0	41.0
94-95	38.12350062525039	41.0	37.0	41.0	32.0	41.0
96-97	38.08579881506984	41.0	37.0	41.0	32.0	41.0
98-99	38.00249622701212	41.0	37.0	41.0	32.0	41.0
100-101	36.89062285009139	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	25.0
13	132.0
14	369.0
15	1651.0
16	8114.0
17	22917.0
18	39711.0
19	53148.0
20	64195.0
21	74975.0
22	87206.0
23	101283.0
24	116129.0
25	133708.0
26	152447.0
27	173679.0
28	199539.0
29	228635.0
30	263684.0
31	307929.0
32	361160.0
33	426992.0
34	520998.0
35	656422.0
36	848334.0
37	1182668.0
38	1955980.0
39	4829294.0
40	1.48352E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.411764014328362	20.39224112593927	12.801346381148493	38.394648478583875
2	27.088989079653587	24.92323425955511	31.820917329934407	16.166859330856894
3	20.855049479680606	28.034580361726295	28.804967616404138	22.30540254218896
4	24.580120973831203	30.940042084931264	23.19080823142914	21.289028709808395
5	28.084564511289024	32.43464562713337	21.875665300958865	17.60512456061874
6	22.42070053878445	37.421955145172085	21.88134458356744	18.27599973247603
7	22.03375545314149	22.08264300540487	35.82605349510007	20.057548046353574
8	22.364898866257807	24.32120473135178	28.45760511915469	24.85629128323572
9	23.59631132234527	22.922203343168423	30.141898678815497	23.339586655670814
10-11	24.78839937083798	30.32763866284351	23.665323642870884	21.218638323447625
12-13	25.42699672849938	25.235835598534422	26.116624783966163	23.220542889000033
14-15	24.635970976723563	26.99707758111099	26.048211175558166	22.318740266607282
16-17	25.658566838399732	26.488807811291355	25.4353649178233	22.41726043248562
18-19	25.214915531182264	27.0332810880609	25.47866754676856	22.273135833988274
20-21	25.484382690196703	27.046876925092604	25.43581474024711	22.03292564446358
22-23	25.283206474630322	26.85195112946533	25.654232639956774	22.210609755947573
24-25	24.727952083275753	26.95450852959192	26.05369505194967	22.263844335182657
26-27	25.018296576641465	27.02255376493111	25.922279408978476	22.03687024944895
28-29	25.204105473850586	26.62721973757457	25.787939895646367	22.38073489292848
30-31	24.861533233443836	26.860623690234537	25.965901299965253	22.311941776356374
32-33	25.070476916758082	27.03788376972951	25.70873054662654	22.18290876688587
34-35	25.223257999794974	26.74098194308686	25.68408935152667	22.3516707055915
36-37	24.934191934692006	26.872259715552303	25.920131827387817	22.27341652236787
38-39	25.13249026163726	27.059380449434734	25.67042421030331	22.1377050786247
40-41	25.305380068652944	26.67317739479294	25.67506631383693	22.346376222717186
42-43	24.89329081956478	26.82400971843883	25.99596109310804	22.286738368888347
44-45	25.072373372936408	26.92211699565381	25.84495348818071	22.160556143229073
46-47	25.240440230560445	26.67423382725156	25.787420458542513	22.297905483645486
48-49	24.89254206293881	26.758153618487757	26.047580023002325	22.30172429557111
50-51	25.237917663512555	26.865559122358285	25.679375293772267	22.217147920356897
52-53	25.367882513158378	26.6838696328188	25.657448694733837	22.290799159288984
54-55	24.948719193962514	26.854605098408303	25.947001709526003	22.249673998103177
56-57	25.021025126110963	27.007284562275828	25.83340640758444	22.138283904028764
58-59	25.273972537083132	26.58799350483671	25.814436116982755	22.323597841097403
60-61	25.00250082955559	26.741723565399646	25.94480942720233	22.31096617784243
62-63	25.16314261495282	26.849349375783927	25.80832964825226	22.179178361010994
64-65	25.285778461378854	26.64618214661743	25.813188324890668	22.254851067113044
66-67	24.954452575043735	26.835859835111197	26.071106940427015	22.138580649418056
68-69	25.25405328789521	26.96443128256255	25.783161205416423	21.99835422412582
70-71	25.4850148089194	26.727453448251037	25.579839062832228	22.207692679997333
72-73	25.00451304028544	26.873165834140885	25.972838487923784	22.1494826376499
74-75	25.115145445064424	26.95880021950778	25.82962737453481	22.09642696089299
76-77	25.38026053288537	26.68631619872603	25.758871114958172	22.174552153430433
78-79	24.987920090453265	26.821584701084415	26.11477840415686	22.075716804305465
80-81	25.263037198084714	26.939165122695837	25.804501416860187	21.993296262359266
82-83	25.407456425225387	26.673029108969352	25.778909784309693	22.14060468149556
84-85	25.109094247234953	26.874119657006524	26.04332187429059	21.973464221467935
86-87	25.239619589201755	27.001294999552467	25.878608174711125	21.880477236534652
88-89	25.3605124849706	26.826967590760837	25.745740784870353	22.06677913939821
90-91	25.0405961010959	26.983499166157603	26.030284770996463	21.94561996175003
92-93	25.348499695097697	27.027327183050375	25.774500139835066	21.84967298201686
94-95	25.618931870693345	26.73103283918481	25.708534522840694	21.941500767281152
96-97	25.187083863199298	26.879051584649517	26.00923641569311	21.92462813645807
98-99	25.392574981904293	27.12282832289764	25.7494138915167	21.735182803681372
100-101	25.67334845010304	26.803459776583754	25.727576010332193	21.795615762981015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	41.0
1	29.0
2	17.5
3	22.5
4	35.5
5	58.5
6	82.5
7	90.5
8	105.0
9	137.0
10	171.0
11	212.5
12	274.0
13	341.5
14	433.0
15	592.0
16	748.5
17	908.5
18	1177.0
19	1526.0
20	1931.5
21	2539.5
22	3419.5
23	4465.5
24	5856.5
25	7866.0
26	10504.0
27	13811.5
28	18504.5
29	24893.0
30	33051.5
31	44342.5
32	60788.5
33	83772.5
34	117881.5
35	171439.5
36	246413.0
37	347268.5
38	491114.0
39	676792.0
40	891594.0
41	1120457.0
42	1354975.0
43	1561724.0
44	1717694.5
45	1809826.0
46	1838786.5
47	1825342.0
48	1749851.0
49	1612945.0
50	1443049.5
51	1284640.0
52	1139320.0
53	983048.5
54	837957.5
55	713805.5
56	585876.5
57	473641.0
58	389717.0
59	325992.5
60	278116.0
61	235510.5
62	197006.5
63	163289.5
64	142080.0
65	122885.5
66	103661.0
67	86532.0
68	67340.5
69	52103.5
70	45436.5
71	38788.5
72	29066.0
73	20646.5
74	12213.5
75	6446.5
76	3693.5
77	1918.0
78	1128.5
79	737.0
80	496.0
81	372.0
82	298.0
83	240.5
84	199.5
85	163.0
86	139.0
87	119.5
88	108.5
89	89.0
90	86.0
91	90.0
92	79.0
93	66.5
94	52.5
95	51.5
96	60.0
97	55.0
98	61.5
99	135.5
100	589.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007411419967298602
2	0.00616714057796199
3	0.0015481150541746223
4	1.7723747115550584E-4
5	0.014320064251115257
6	0.0014576877729728338
7	0.005244782309703744
8	0.00935018087626495
9	0.003447087959412185
10-11	0.006570446252121967
12-13	0.004555726426946114
14-15	0.009559972168653102
16-17	1.175554655623253E-4
18-19	0.012705033008851311
20-21	0.0063299096841252095
22-23	0.008615911352906427
24-25	0.011690438913767242
26-27	0.009735401094184571
28-29	0.006767577725141865
30-31	0.010769889191133034
32-33	0.005351486501521855
34-35	0.004984351739842592
36-37	0.009028259755186584
38-39	0.010447968070054665
40-41	0.00870453008848418
42-43	0.012301727334691334
44-45	0.00798111183886987
46-47	0.004311572767701285
48-49	0.01012966404022437
50-51	0.010397328792581664
52-53	0.0074747190641398535
54-55	0.010476904800039238
56-57	0.012327046973427836
58-59	0.005951923648701732
60-61	0.0025229211455299046
62-63	0.012775566288188706
64-65	0.005139886663509669
66-67	0.006192460216698489
68-69	0.015345509619943543
70-71	0.006141820939225488
72-73	0.005912135644972945
74-75	0.013627391277109557
76-77	0.00636788914222996
78-79	0.012728544101963776
80-81	2.351109311246506E-5
82-83	0.007246842315511346
84-85	0.016635002649881048
86-87	0.002056316374528675
88-89	0.010079024762751368
90-91	0.003660496343048406
92-93	0.01173022691749603
94-95	0.010167643498329122
96-97	0.004224762577747568
98-99	0.009178369041981552
100-101	0.010241793868914589
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.7646524E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.33208742058996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.64431182537334	24.133071003087142
2	15.080254493235174	11.259547581323998
3	6.743396017547075	7.552351489161769
4	3.642182223621013	5.438810606957535
5	2.297640096714791	4.2887850475804665
6	1.5359395220670926	3.4403897106328714
7	1.0718867302258215	2.801103838243248
8	0.7783014504993379	2.3244494231690593
9	0.581282256036137	1.9530431998540942
>10	3.3077934516246876	22.16232892604215
>50	0.20785000913363813	5.289636138361773
>100	0.10174816719946916	7.022455157970271
>500	0.00601896477659129	1.4977073354227841
>1k	0.001384656241266139	0.7986786026453828
>5k	1.0135704538901466E-5	0.037641939547437914
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.808545624035774E-5	0.0	1.808545624035774E-5	1.0851273744214643E-5	0.0
2	2.1702547488429287E-5	3.6170912480715477E-6	3.978800372878703E-5	1.808545624035774E-5	0.0
3	2.1702547488429287E-5	3.6170912480715477E-6	4.3405094976858574E-5	1.808545624035774E-5	0.0
4	6.510764246528786E-5	3.6170912480715477E-6	5.787345996914476E-5	1.808545624035774E-5	0.0
5	9.04272812017887E-5	3.6170912480715477E-6	5.787345996914476E-5	2.1702547488429287E-5	0.0
6	1.3021528493057573E-4	3.6170912480715477E-6	7.234182496143096E-5	2.5319638736500834E-5	0.0
7	1.4468364992286192E-4	3.6170912480715477E-6	7.957600745757405E-5	2.5319638736500834E-5	0.0
8	1.663861974112912E-4	3.6170912480715477E-6	9.04272812017887E-5	2.893672998457238E-5	0.0
9	1.8808874489972048E-4	7.234182496143095E-6	1.0127855494600334E-4	2.893672998457238E-5	0.0
10-11	2.2606820300447174E-4	7.234182496143095E-6	1.4287510429882613E-4	3.4362366856679706E-5	3.6170912480715477E-6
12-13	2.803245717255449E-4	7.234182496143095E-6	1.862801992756847E-4	3.617091248071548E-5	3.6170912480715477E-6
14-15	3.472407598148686E-4	1.0851273744214643E-5	2.1340838363622132E-4	3.617091248071548E-5	3.6170912480715477E-6
16-17	4.2681676727244263E-4	1.446836499228619E-5	2.984100279659027E-4	3.617091248071548E-5	7.234182496143095E-6
18-19	5.190525940982671E-4	1.446836499228619E-5	3.3277239482258235E-4	3.617091248071548E-5	7.234182496143095E-6
20-21	6.72778972141308E-4	1.446836499228619E-5	3.8341167229558406E-4	3.797945810475125E-5	7.234182496143095E-6
22-23	8.608677170410284E-4	1.446836499228619E-5	4.919244097377305E-4	3.978800372878703E-5	7.234182496143095E-6
24-25	0.0011809802924953603	1.446836499228619E-5	5.190525940982671E-4	3.978800372878703E-5	7.234182496143095E-6
26-27	0.0016331166985043039	2.1702547488429287E-5	5.317124134665175E-4	3.978800372878703E-5	7.234182496143095E-6
28-29	0.0022552563931726097	2.5319638736500834E-5	5.642662346991615E-4	3.978800372878703E-5	7.234182496143095E-6
30-31	0.002924418274065846	2.8936729984572385E-5	5.67883325947233E-4	4.15965493528228E-5	1.0851273744214643E-5
32-33	0.0036604963430484066	3.255382123264393E-5	6.004371471798769E-4	4.3405094976858574E-5	1.446836499228619E-5
34-35	0.004479767510736612	3.255382123264393E-5	6.818217002614868E-4	4.702218622493012E-5	1.446836499228619E-5
36-37	0.0054708505127082155	3.255382123264393E-5	7.179926127422022E-4	4.702218622493012E-5	2.5319638736500834E-5
38-39	0.006669916261443934	3.255382123264393E-5	7.632062533430966E-4	4.702218622493012E-5	2.5319638736500834E-5
40-41	0.008248776591227164	3.255382123264393E-5	7.975686201997763E-4	4.702218622493012E-5	2.5319638736500834E-5
42-43	0.010075407671503297	3.617091248071548E-5	8.771446276573503E-4	4.702218622493012E-5	2.7128184360536608E-5
44-45	0.01210640440729547	3.797945810475125E-5	8.970386295217439E-4	5.063927747300167E-5	3.255382123264393E-5
46-47	0.014441236807925653	3.978800372878703E-5	9.404437244986024E-4	5.787345996914477E-5	3.978800372878703E-5
48-49	0.017175757791467745	3.978800372878703E-5	9.874659107235325E-4	6.149055121721632E-5	3.978800372878703E-5
50-51	0.02053422701530218	3.978800372878703E-5	9.983171844677472E-4	6.149055121721632E-5	3.978800372878703E-5
52-53	0.024525687207549128	4.3405094976858574E-5	0.001047147916316713	6.691618808932364E-5	3.978800372878703E-5
54-55	0.029267693833770928	4.3405094976858574E-5	0.0011014042850377862	6.872473371335941E-5	3.978800372878703E-5
56-57	0.03457758378593996	4.521364060089435E-5	0.0011484264712627165	6.872473371335941E-5	4.3405094976858574E-5
58-59	0.04067961672143666	4.702218622493012E-5	0.0011737461099992172	6.872473371335941E-5	4.3405094976858574E-5
60-61	0.04834423307610027	4.8830731848965895E-5	0.0012171512049760757	6.872473371335941E-5	4.3405094976858574E-5
62-63	0.0580977196265252	5.244782309703744E-5	0.0013256639424182224	6.872473371335941E-5	4.3405094976858574E-5
64-65	0.07007571729451413	5.787345996914477E-5	0.0014124741323719393	6.872473371335941E-5	5.244782309703744E-5
66-67	0.0837284282103602	6.510764246528786E-5	0.0014251339517401898	6.872473371335941E-5	5.787345996914476E-5
68-69	0.10022598139281452	7.59589162095025E-5	0.0014323681342363328	6.872473371335941E-5	5.787345996914476E-5
70-71	0.12007657816223118	7.59589162095025E-5	0.001439602316732476	6.872473371335941E-5	6.329909684125209E-5
72-73	0.14476684302156756	7.59589162095025E-5	0.0014848159573333704	6.872473371335941E-5	6.510764246528786E-5
74-75	0.1763512837997283	7.776746183353828E-5	0.0014866245029574062	6.872473371335941E-5	6.872473371335941E-5
76-77	0.21531459072395503	7.957600745757405E-5	0.001488433048581442	6.872473371335941E-5	6.872473371335941E-5
78-79	0.26124984102883964	8.31930987056456E-5	0.0014920501398295134	6.872473371335941E-5	6.872473371335941E-5
80-81	0.31663655076493524	8.861873557775292E-5	0.0014974757767016208	6.872473371335941E-5	6.872473371335941E-5
82-83	0.3832760313737814	9.404437244986024E-5	0.0015191783241900502	6.872473371335941E-5	6.872473371335941E-5
84-85	0.4671437175971923	9.404437244986024E-5	0.0015336466891823363	6.872473371335941E-5	6.872473371335941E-5
86-87	0.5727356538565209	9.404437244986024E-5	0.001551732145422694	6.872473371335941E-5	6.872473371335941E-5
88-89	0.6996195977476228	1.0308710057003911E-4	0.0015625834191669086	7.234182496143096E-5	6.872473371335941E-5
90	0.8083330837540372	1.0489564619407488E-4	0.0015625834191669086	7.234182496143096E-5	7.234182496143096E-5
>>END_MODULE
