##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654132_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30971893
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.338962749225566	32.0	32.0	32.0	32.0	32.0
2	31.395863049120052	32.0	32.0	32.0	32.0	32.0
3	34.65103976692674	37.0	32.0	37.0	32.0	37.0
4	35.796696572598904	37.0	37.0	37.0	32.0	37.0
5	35.92337417025172	37.0	37.0	37.0	37.0	37.0
6	39.107805357586635	41.0	41.0	41.0	37.0	41.0
7	39.511321087154734	41.0	41.0	41.0	37.0	41.0
8	39.59979298004161	41.0	41.0	41.0	37.0	41.0
9	39.51017391801011	41.0	41.0	41.0	37.0	41.0
10-11	39.50467770891498	41.0	41.0	41.0	37.0	41.0
12-13	39.55549534863755	41.0	41.0	41.0	37.0	41.0
14-15	39.598126904932805	41.0	41.0	41.0	37.0	41.0
16-17	39.41058890071717	41.0	41.0	41.0	37.0	41.0
18-19	39.46947676075208	41.0	41.0	41.0	37.0	41.0
20-21	39.45118616094922	41.0	41.0	41.0	37.0	41.0
22-23	39.28693627154142	41.0	41.0	41.0	37.0	41.0
24-25	39.292754788995296	41.0	41.0	41.0	37.0	41.0
26-27	39.22257620804773	41.0	41.0	41.0	37.0	41.0
28-29	39.12468077104619	41.0	41.0	41.0	37.0	41.0
30-31	39.28084211707693	41.0	41.0	41.0	37.0	41.0
32-33	39.38566657840384	41.0	41.0	41.0	37.0	41.0
34-35	39.28035475584266	41.0	41.0	41.0	37.0	41.0
36-37	39.07309367561098	41.0	41.0	41.0	37.0	41.0
38-39	39.091897627955774	41.0	41.0	41.0	37.0	41.0
40-41	39.04909783848213	41.0	41.0	41.0	37.0	41.0
42-43	38.92688606408397	41.0	41.0	41.0	37.0	41.0
44-45	38.946725422950415	41.0	41.0	41.0	37.0	41.0
46-47	38.92051309230598	41.0	41.0	41.0	34.5	41.0
48-49	38.95281907696116	41.0	41.0	41.0	34.5	41.0
50-51	38.859566284824766	41.0	41.0	41.0	32.0	41.0
52-53	38.93767432943153	41.0	41.0	41.0	34.5	41.0
54-55	38.81230089810784	41.0	41.0	41.0	32.0	41.0
56-57	38.79712436369324	41.0	41.0	41.0	32.0	41.0
58-59	38.785616090692294	41.0	41.0	41.0	32.0	41.0
60-61	38.80427374910536	41.0	41.0	41.0	32.0	41.0
62-63	38.81643640574375	41.0	41.0	41.0	32.0	41.0
64-65	38.81090120968712	41.0	41.0	41.0	32.0	41.0
66-67	38.757165650158996	41.0	41.0	41.0	32.0	41.0
68-69	38.7066070840423	41.0	41.0	41.0	32.0	41.0
70-71	38.656753996276564	41.0	41.0	41.0	32.0	41.0
72-73	38.612554437663846	41.0	41.0	41.0	32.0	41.0
74-75	38.586010855067855	41.0	41.0	41.0	32.0	41.0
76-77	37.77063156908104	41.0	39.0	41.0	29.5	41.0
78-79	38.32584821341079	41.0	39.0	41.0	32.0	41.0
80-81	38.74262901851043	41.0	41.0	41.0	32.0	41.0
82-83	38.781599222882505	41.0	41.0	41.0	32.0	41.0
84-85	38.65723829021364	41.0	41.0	41.0	32.0	41.0
86-87	38.69399204950114	41.0	41.0	41.0	32.0	41.0
88-89	38.5726534216039	41.0	41.0	41.0	32.0	41.0
90-91	38.47524095475856	41.0	41.0	41.0	32.0	41.0
92-93	38.45654642420468	41.0	41.0	41.0	32.0	41.0
94-95	38.282026997833164	41.0	37.0	41.0	32.0	41.0
96-97	38.24653506003007	41.0	37.0	41.0	32.0	41.0
98-99	38.169183572344124	41.0	37.0	41.0	32.0	41.0
100-101	37.0725454688869	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	50.0
13	122.0
14	421.0
15	1547.0
16	7111.0
17	19064.0
18	33963.0
19	46733.0
20	57565.0
21	69771.0
22	83413.0
23	97935.0
24	115256.0
25	133945.0
26	154839.0
27	180563.0
28	208416.0
29	241581.0
30	282721.0
31	331872.0
32	388191.0
33	460860.0
34	564780.0
35	714022.0
36	926803.0
37	1286338.0
38	2139062.0
39	5329010.0
40	1.7095939E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	27.697349261771198	20.516013139207907	12.880728613890028	38.90590898513087
2	27.11838033540634	25.05660878390864	31.602924208921163	16.222086671763854
3	20.733802211935078	28.12313950996571	28.74455912357433	22.398499154524877
4	24.426941445348653	30.81001497005263	23.336544315181843	21.426499269416873
5	28.16860479478709	32.23057017046591	22.064060674341547	17.53676436040545
6	22.54189434834133	37.317590303654455	21.895918175662654	18.24459717234156
7	22.20698646220906	22.19829425429235	35.57304494662574	20.021674336872845
8	22.510410018545986	24.38636874044709	28.397361681283183	24.70585955972374
9	23.730860701449675	22.949829052057755	30.072853709580677	23.246456536911893
10-11	24.893325213603045	30.264683403170118	23.55611412784636	21.285877255380477
12-13	25.543980423123795	25.24768798714231	25.95196654768861	23.256365042045285
14-15	24.758060474612982	27.0275327218639	25.87788566954405	22.336521133979073
16-17	25.782109638794175	26.493946205646406	25.28054299877051	22.443401156788905
18-19	25.30625875888248	27.025538648163778	25.341898948163948	22.326303644789792
20-21	25.626221559492485	27.026930464185224	25.28849513654222	22.05835283978007
22-23	25.403717431162033	26.867938871240415	25.486306059792497	22.242037637805062
24-25	24.82216835692232	27.00405690418831	25.88383016833884	22.289944570550528
26-27	25.17170049345224	27.006908447586138	25.758789052913723	22.0626020060479
28-29	25.326008443723307	26.643455307194923	25.625449026873	22.40508722220877
30-31	24.98499076535496	26.846923760385756	25.814236751295905	22.353848722963377
32-33	25.208127480615556	27.04704479166248	25.551858837226803	22.19296889049516
34-35	25.32918376839241	26.784890628332324	25.522704564031717	22.363221039243548
36-37	25.041147947701255	26.864029679446165	25.77082503505961	22.32399733779297
38-39	25.263823747621462	27.05037736297472	25.519259965063956	22.16653892433986
40-41	25.444485044058112	26.662689798888543	25.520514063439244	22.372311093614098
42-43	25.042552828039106	26.82224286074612	25.812000893697384	22.323203417517394
44-45	25.205880622102327	26.903903432886896	25.722995249253355	22.167220695757425
46-47	25.363438442236973	26.675481610524177	25.63391668714044	22.327163260098402
48-49	25.01075883623967	26.758814023713374	25.887054039880645	22.34337310016631
50-51	25.352897477595548	26.861743882340793	25.57087453605465	22.214484104009006
52-53	25.48163479097428	26.657865978967504	25.537479968831967	22.323019261226257
54-55	25.05894474698994	26.838828678209932	25.791633179140156	22.31059339565997
56-57	25.140582910346087	27.01821049711241	25.707446658819087	22.133759933722413
58-59	25.393271625673187	26.598277811173887	25.662262523867042	22.34618803928588
60-61	25.102810195940418	26.784165263479075	25.773616736866405	22.3394078037141
62-63	25.28075451681002	26.856810235636186	25.684424833493825	22.178010414059965
64-65	25.368427889408206	26.668096747676444	25.678903029383964	22.284572333531376
66-67	25.064475861556456	26.846183523830792	25.913041083230315	22.17629953138244
68-69	25.35698792269736	26.98118229933309	25.66335262418262	21.99847715378693
70-71	25.60038416604694	26.752777366402192	25.42262147960467	22.2242169879462
72-73	25.135124121877254	26.893725110626253	25.822465734953425	22.14868503254306
74-75	25.251385627321906	26.977346489207	25.68045233912828	22.090815544342817
76-77	25.496671463687072	26.701324275339566	25.630620796909415	22.17138346406395
78-79	25.113316666377656	26.824488987180032	25.977186414186864	22.085007932255447
80-81	25.387828682914915	26.938864129072133	25.67870695526319	21.994600232749757
82-83	25.545215851085008	26.683281338821942	25.643218360964447	22.128284449128603
84-85	25.263014191648736	26.87652376740089	25.89556935123142	21.96489268971896
86-87	25.389493532162057	27.022056200128336	25.74002996198474	21.848420305724865
88-89	25.50859025497303	26.852939345872727	25.59717333656671	22.041297062587535
90-91	25.206085366787466	26.982748852008502	25.872477656033944	21.93868812517009
92-93	25.523001574619368	27.061784088339362	25.61452957475396	21.800684762287307
94-95	25.77774850086321	26.783337862106055	25.538629301143366	21.900284335887367
96-97	25.36104021803555	26.909834377957598	25.837334702419113	21.89179070158774
98-99	25.59085937683904	27.147776914510768	25.586890881589436	21.674472827060757
100-101	25.870191193973106	26.833944736506794	25.55900718190723	21.736856887612873
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	46.5
2	26.0
3	25.5
4	34.0
5	54.0
6	65.0
7	92.0
8	126.0
9	151.0
10	180.5
11	222.0
12	272.0
13	356.0
14	452.5
15	565.5
16	705.0
17	868.5
18	1109.0
19	1379.0
20	1751.5
21	2287.5
22	3037.0
23	4095.0
24	5357.0
25	7141.5
26	9501.0
27	12719.0
28	17326.5
29	23294.0
30	31276.0
31	42573.5
32	58693.5
33	80982.5
34	116229.5
35	172674.5
36	251259.5
37	356808.0
38	510116.5
39	714290.5
40	956002.5
41	1217008.0
42	1489032.0
43	1735208.0
44	1926185.5
45	2045080.0
46	2092154.5
47	2084590.5
48	2003417.0
49	1852676.5
50	1658338.5
51	1476986.5
52	1309615.5
53	1127552.5
54	960826.5
55	817822.5
56	670156.0
57	539273.0
58	441994.5
59	368998.5
60	312463.5
61	263624.0
62	219799.5
63	180222.0
64	155266.0
65	132821.5
66	110875.0
67	92115.0
68	71126.5
69	54469.5
70	47483.5
71	41012.5
72	31087.0
73	22183.0
74	13832.0
75	8163.5
76	4855.5
77	2521.0
78	1462.0
79	920.0
80	605.0
81	447.0
82	338.5
83	243.5
84	188.5
85	176.5
86	153.0
87	126.5
88	112.5
89	101.0
90	88.0
91	73.5
92	65.5
93	58.0
94	60.5
95	65.0
96	62.0
97	67.0
98	78.0
99	152.0
100	670.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007406715501696974
2	0.006057104743323245
3	0.0017758036294391176
4	1.872665645590342E-4
5	0.014432440406532463
6	0.0015239623874459336
7	0.0052499212753963735
8	0.00945373277635952
9	0.003425686637881643
10-11	0.006660877977332544
12-13	0.004768839928511958
14-15	0.009676515413507336
16-17	1.1784878631732325E-4
18-19	0.013056999777185076
20-21	0.00646876831196595
22-23	0.008841887707670953
24-25	0.011939857857574286
26-27	0.010141453091033216
28-29	0.006919176687069145
30-31	0.011166576095300342
32-33	0.005267679311690764
34-35	0.005094942049554413
36-37	0.009206734635173897
38-39	0.010658050510506412
40-41	0.00903076863916584
42-43	0.012643721841606517
44-45	0.008234885739789944
46-47	0.004455652742956331
48-49	0.010210870869274927
50-51	0.010548273558868358
52-53	0.0075665378283464955
54-55	0.010566031595162749
56-57	0.012777714297282377
58-59	0.00608939208204032
60-61	0.0023989492666786627
62-63	0.013068300345736052
64-65	0.0052289345052302745
66-67	0.006358991360327895
68-69	0.015752992560060824
70-71	0.00630087415063716
72-73	0.005969928928787143
74-75	0.013983646398365123
76-77	0.006373520662750578
78-79	0.013011797502981171
80-81	8.071834679268717E-6
82-83	0.007355055759749655
84-85	0.01700089820147577
86-87	0.0020889908149947438
88-89	0.010414281103192497
90-91	0.003782461730705321
92-93	0.012185241631824054
94-95	0.010454640276588842
96-97	0.0043507188921258376
98-99	0.00960871200220148
100-101	0.010457869010460549
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.0971893E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.657319674036984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.06009789723601	21.854939715005763
2	15.247373769941905	10.568662138688062
3	6.874915699114219	7.147984533487708
4	3.947469920908885	5.472349078103389
5	2.364290760145658	4.096999033836999
6	1.6002587804638202	3.3276408069431516
7	1.1965349927384565	2.9028087021155744
8	0.8763851418738903	2.429852801559974
9	0.6546152523500475	2.041848905777639
>10	3.8004588075444588	23.797447545602502
>50	0.2461633068671284	5.817645613344247
>100	0.12200382036833068	7.653267632942241
>500	0.007343442866421388	1.6959201230560428
>1k	0.002059132590008678	1.106281384329619
>5k	1.951666036759248E-5	0.045369203836360165
>10k+	9.75833018379624E-6	0.04098278137077991
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.520227420390481E-5	0.0	4.520227420390481E-5	3.2287338717074866E-6	0.0
2	5.1659741947319786E-5	3.2287338717074866E-6	6.457467743414973E-5	6.457467743414973E-6	0.0
3	7.10321451775647E-5	3.2287338717074866E-6	6.457467743414973E-5	6.457467743414973E-6	3.2287338717074866E-6
4	1.2269188712488448E-4	3.2287338717074866E-6	8.717581453610213E-5	6.457467743414973E-6	3.2287338717074866E-6
5	1.3560682261171443E-4	3.2287338717074866E-6	9.040454840780963E-5	9.68620161512246E-6	9.68620161512246E-6
6	1.7435162907220427E-4	3.2287338717074866E-6	1.0977695163805455E-4	9.68620161512246E-6	9.68620161512246E-6
7	1.937240323024492E-4	3.2287338717074866E-6	1.5175049197025187E-4	9.68620161512246E-6	9.68620161512246E-6
8	2.195539032761091E-4	1.2914935486829947E-5	1.6143669358537432E-4	9.68620161512246E-6	9.68620161512246E-6
9	2.38926306506354E-4	1.2914935486829947E-5	1.7435162907220427E-4	9.68620161512246E-6	9.68620161512246E-6
10-11	2.841285807102588E-4	1.2914935486829947E-5	1.904952984307417E-4	9.68620161512246E-6	1.2914935486829947E-5
12-13	3.3255958878587114E-4	1.6143669358537433E-5	2.163251694044016E-4	9.68620161512246E-6	1.7758036294391177E-5
14-15	3.9067679847660586E-4	1.6143669358537433E-5	2.469981411856227E-4	9.68620161512246E-6	1.937240323024492E-5
16-17	4.810813468844155E-4	1.7758036294391177E-5	3.5193199201611605E-4	1.1300568550976203E-5	2.2601137101952407E-5
18-19	6.360605727263748E-4	2.2601137101952407E-5	4.197354033219732E-4	1.2914935486829947E-5	2.2601137101952407E-5
20-21	8.540001090666302E-4	2.5829870973659893E-5	4.7785261301270805E-4	1.2914935486829947E-5	2.2601137101952407E-5
22-23	0.0011090700849315216	2.905860484536738E-5	5.714858952922252E-4	1.2914935486829947E-5	2.2601137101952407E-5
24-25	0.00152234802051008	3.390170565292861E-5	5.924726654583238E-4	1.6143669358537433E-5	2.2601137101952407E-5
26-27	0.002030873605304009	3.874480646048984E-5	6.199169033678374E-4	1.937240323024492E-5	2.2601137101952407E-5
28-29	0.002623346270762333	4.520227420390481E-5	6.586617098283272E-4	2.2601137101952407E-5	2.2601137101952407E-5
30-31	0.0034337584725609117	4.681664113975856E-5	6.699622783793034E-4	2.2601137101952407E-5	2.2601137101952407E-5
32-33	0.004328117755023886	5.327410888317353E-5	6.812628469302797E-4	2.2601137101952407E-5	2.2601137101952407E-5
34-35	0.0052289345052302745	5.811720969073476E-5	8.249415042212629E-4	2.2601137101952407E-5	2.2601137101952407E-5
36-37	0.006336390223225942	5.811720969073476E-5	8.701437784251676E-4	2.2601137101952407E-5	2.2601137101952407E-5
38-39	0.00781030723566041	5.811720969073476E-5	9.105029518215112E-4	2.2601137101952407E-5	2.2601137101952407E-5
40-41	0.009695887816737582	5.811720969073476E-5	9.508621252178548E-4	2.5829870973659893E-5	2.2601137101952407E-5
42-43	0.011896269950306234	5.811720969073476E-5	0.0010218942703954194	2.5829870973659893E-5	2.7444237909513636E-5
44-45	0.01442113983798149	5.811720969073476E-5	0.0010331948389463957	2.5829870973659893E-5	2.905860484536738E-5
46-47	0.017059015411166504	6.134594356244224E-5	0.0011058413510598142	2.5829870973659893E-5	3.2287338717074866E-5
48-49	0.020420127371614	6.134594356244224E-5	0.0011365143228410353	2.5829870973659893E-5	3.2287338717074866E-5
50-51	0.02461586703789788	6.296031049829599E-5	0.001157501093007134	2.5829870973659893E-5	3.2287338717074866E-5
52-53	0.029817357305218638	6.618904437000347E-5	0.0011833309639807939	2.7444237909513636E-5	3.2287338717074866E-5
54-55	0.03575015579448114	6.780341130585721E-5	0.001214003935762015	2.905860484536738E-5	3.2287338717074866E-5
56-57	0.04249013775167052	6.780341130585721E-5	0.0012559774760942124	2.905860484536738E-5	3.2287338717074866E-5
58-59	0.050450581112365334	6.780341130585721E-5	0.001276964246260311	2.905860484536738E-5	3.2287338717074866E-5
60-61	0.06014324019523121	6.780341130585721E-5	0.0013366958228868994	2.905860484536738E-5	3.2287338717074866E-5
62-63	0.07274821723037724	6.780341130585721E-5	0.0014690739116269063	3.067297178122112E-5	3.2287338717074866E-5
64-65	0.08771178435880558	7.103214517756469E-5	0.0015449491576120323	3.2287338717074866E-5	4.0359173396343586E-5
66-67	0.10549887925804212	7.910397985683341E-5	0.001553020992291301	3.2287338717074866E-5	4.520227420390481E-5
68-69	0.12665192921853372	1.0009075002293208E-4	0.0015869226979442295	3.2287338717074866E-5	4.520227420390481E-5
70-71	0.15238332380910652	1.0170511695878583E-4	0.0015982232664952059	3.2287338717074866E-5	5.327410888317353E-5
72-73	0.184310658699486	1.0331948389463957E-4	0.0016337393390839883	3.551607258878235E-5	5.650284275488101E-5
74-75	0.22534625184195234	1.049338508304933E-4	0.0016369680729556957	4.520227420390481E-5	5.811720969073476E-5
76-77	0.27501870809123613	1.0654821776634705E-4	0.0016450399076349642	4.520227420390481E-5	5.811720969073476E-5
78-79	0.33461467789521293	1.1139131857390829E-4	0.0016498830084425255	5.327410888317353E-5	5.811720969073476E-5
80-81	0.4053304071533503	1.1300568550976203E-4	0.0016498830084425255	5.811720969073476E-5	6.134594356244224E-5
82-83	0.49156020266504213	1.1300568550976203E-4	0.001672484145544478	5.811720969073476E-5	6.134594356244224E-5
84-85	0.5984538949556619	1.2269188712488448E-4	0.0016886278149030156	5.811720969073476E-5	6.134594356244224E-5
86-87	0.7307512653488761	1.3722118954756816E-4	0.0016934709157105767	5.811720969073476E-5	6.134594356244224E-5
88-89	0.8892627260464836	1.3883555648342192E-4	0.001698314016518138	6.134594356244224E-5	6.457467743414973E-5
90	1.0253845317107353	1.420642903551294E-4	0.0017015427503898454	6.134594356244224E-5	6.457467743414973E-5
>>END_MODULE
