##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654116_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30594634
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.603492887020646	32.0	32.0	32.0	32.0	32.0
2	31.605399757356142	32.0	32.0	32.0	32.0	32.0
3	35.68598852988403	37.0	37.0	37.0	32.0	37.0
4	36.31975620299952	37.0	37.0	37.0	37.0	37.0
5	36.51155029342727	37.0	37.0	37.0	37.0	37.0
6	40.078764857915935	41.0	41.0	41.0	37.0	41.0
7	40.1143050771583	41.0	41.0	41.0	37.0	41.0
8	40.20954887056338	41.0	41.0	41.0	37.0	41.0
9	40.2279151304768	41.0	41.0	41.0	37.0	41.0
10-11	40.28675003923891	41.0	41.0	41.0	41.0	41.0
12-13	40.260335554267456	41.0	41.0	41.0	41.0	41.0
14-15	40.21188267851153	41.0	41.0	41.0	41.0	41.0
16-17	40.23621555662342	41.0	41.0	41.0	41.0	41.0
18-19	40.23030708587656	41.0	41.0	41.0	41.0	41.0
20-21	40.21089103402904	41.0	41.0	41.0	41.0	41.0
22-23	40.201650524729274	41.0	41.0	41.0	41.0	41.0
24-25	40.185061161378826	41.0	41.0	41.0	41.0	41.0
26-27	40.13287727187715	41.0	41.0	41.0	41.0	41.0
28-29	40.10951105020573	41.0	41.0	41.0	37.0	41.0
30-31	40.086388351630546	41.0	41.0	41.0	37.0	41.0
32-33	40.08083455092158	41.0	41.0	41.0	37.0	41.0
34-35	40.0702272496543	41.0	41.0	41.0	37.0	41.0
36-37	39.9684662186186	41.0	41.0	41.0	37.0	41.0
38-39	40.0044525781874	41.0	41.0	41.0	37.0	41.0
40-41	40.01140219556149	41.0	41.0	41.0	37.0	41.0
42-43	39.997152752342124	41.0	41.0	41.0	37.0	41.0
44-45	39.97980101674038	41.0	41.0	41.0	37.0	41.0
46-47	39.957095319394895	41.0	41.0	41.0	37.0	41.0
48-49	39.93673451037198	41.0	41.0	41.0	37.0	41.0
50-51	39.87339232428798	41.0	41.0	41.0	37.0	41.0
52-53	39.87144665629927	41.0	41.0	41.0	37.0	41.0
54-55	39.832224386145626	41.0	41.0	41.0	37.0	41.0
56-57	39.818667956609644	41.0	41.0	41.0	37.0	41.0
58-59	39.78568550615771	41.0	41.0	41.0	37.0	41.0
60-61	39.78272469283339	41.0	41.0	41.0	37.0	41.0
62-63	39.73461918191275	41.0	41.0	41.0	37.0	41.0
64-65	39.73978368232808	41.0	41.0	41.0	37.0	41.0
66-67	39.68232443963866	41.0	41.0	41.0	37.0	41.0
68-69	39.65006520424464	41.0	41.0	41.0	37.0	41.0
70-71	39.648290481265434	41.0	41.0	41.0	37.0	41.0
72-73	39.619898051403396	41.0	41.0	41.0	37.0	41.0
74-75	39.5771637111266	41.0	41.0	41.0	37.0	41.0
76-77	39.020024753360346	41.0	39.0	41.0	34.5	41.0
78-79	39.38055908758379	41.0	41.0	41.0	37.0	41.0
80-81	39.66408883989264	41.0	41.0	41.0	37.0	41.0
82-83	39.68805018226399	41.0	41.0	41.0	37.0	41.0
84-85	39.70206969954273	41.0	41.0	41.0	37.0	41.0
86-87	39.68217058913011	41.0	41.0	41.0	37.0	41.0
88-89	39.63844234907337	41.0	41.0	41.0	37.0	41.0
90-91	39.60502534529421	41.0	41.0	41.0	37.0	41.0
92-93	39.555578488044674	41.0	41.0	41.0	37.0	41.0
94-95	39.5306636778201	41.0	41.0	41.0	37.0	41.0
96-97	39.51004640225472	41.0	41.0	41.0	37.0	41.0
98-99	39.46293237238922	41.0	41.0	41.0	37.0	41.0
100-101	38.93228225903928	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	2.0
16	8.0
17	22.0
18	57.0
19	197.0
20	570.0
21	1753.0
22	4353.0
23	9115.0
24	16909.0
25	28423.0
26	46612.0
27	71712.0
28	104030.0
29	143116.0
30	188787.0
31	238205.0
32	293127.0
33	357412.0
34	430095.0
35	521096.0
36	648094.0
37	858222.0
38	1288196.0
39	2881134.0
40	2.2463387E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.82214894250578	11.006890722828874	5.652780645715602	44.51817968894975
2	24.090280014462667	12.576277264830166	31.212810063359477	32.12063265734769
3	20.652904805542434	14.029181088375587	20.95184978559795	44.36606432048403
4	23.837209492357385	21.10639074812923	21.022496951589613	34.033902807923766
5	25.031945144367473	25.845931021760222	23.882946270904892	25.239177562967413
6	20.87806966411169	30.072178670285776	25.451884797837426	23.597866867765113
7	16.013870275421503	25.346052513653213	40.09038970690089	18.549687504024398
8	18.407682272668083	24.830668269052257	32.25343579396794	24.508213664311725
9	18.370757434130443	22.745799148961872	34.94555287048049	23.937890546427194
10-11	20.92251863513059	31.076653834133133	25.99143692975703	22.00939060097924
12-13	21.514763013670958	25.456472203589687	28.115639494167503	24.913125288571848
14-15	21.09222886204139	26.02192524045338	27.765168135631868	25.120677761873356
16-17	21.62128005845731	26.34746014611582	27.02345777367364	25.00780202175323
18-19	21.37721601768467	26.569592236337915	26.88638635127977	25.166805394697644
20-21	21.6249963964269	26.51496827842425	27.042346706942137	24.81768861820671
22-23	21.626781023103593	26.691525383176675	26.92262309789357	24.759070495826162
24-25	21.54785949539584	26.464428286215835	26.703231351731972	25.28448086665635
26-27	21.393142013073273	26.56880451650443	26.980685567279544	25.05736790314275
28-29	21.442439035375827	26.778774353744144	26.832043866295503	24.946742744584526
30-31	21.50184735009414	26.43606555319472	26.899076485111735	25.163010611599407
32-33	21.612209972197245	26.3919112737148	26.90144511137518	25.094433642712776
34-35	21.714832748075025	26.530017435587226	26.59710604911934	25.158043767218413
36-37	21.54085255603973	26.4172828477046	26.51109831874439	25.53076627751128
38-39	21.59273256872431	26.443924774520916	26.85750873829705	25.105833918457726
40-41	21.745888511037588	26.708145944808493	26.677465728140433	24.868499816013486
42-43	21.75862277025442	26.35059795126165	26.66382934994418	25.226949928539753
44-45	21.708161306979516	26.321887034177298	26.836205983049183	25.133745675793996
46-47	21.72184017628712	26.47208820997826	26.657637087601703	25.148434526132917
48-49	21.64208436028357	26.281602192070675	26.716163690665496	25.36014975698026
50-51	21.6025594553607	26.31060891266096	26.852754636646413	25.23407699533193
52-53	22.056923446118034	26.436613034821725	26.489720386914904	25.01674313214533
54-55	21.90262187807182	26.30929495675614	26.463174882235883	25.324908282936153
56-57	21.75844463444145	26.231683307602243	26.87372563437105	25.13614642358526
58-59	21.793275905833685	26.34525060832563	26.75900453654716	25.102468949293527
60-61	21.696427223152924	26.157675231545507	26.7527044121528	25.393193133148774
62-63	21.756666544858813	26.14587414250486	26.91895088530884	25.178508427327483
64-65	21.960306830276185	26.313573484814363	26.64168004101634	25.08443964389311
66-67	21.782682544919478	26.194498682350638	26.625322597420187	25.397496175309698
68-69	21.808571071646092	26.156220728118534	26.856176805383587	25.17903139485179
70-71	21.990609529762636	26.405967137897452	26.66858998868887	24.934833343651047
72-73	21.971252724457674	26.278348079552938	26.486327449521273	25.264071746468115
74-75	21.92460397228909	26.202736117171575	26.743821183253825	25.1288387272855
76-77	21.912921069753605	26.29589391394582	26.651794559791103	25.139390456509464
78-79	21.805248899843267	26.16379591486461	26.67684148318028	25.354113702111842
80-81	21.877995010497592	26.16748250689974	26.818345007820653	25.136177474782016
82-83	21.976714936351257	26.43056458854844	26.530106553979365	25.062613921120942
84-85	22.05556699910187	26.16247672712803	26.497932611320014	25.28402366245009
86-87	22.00829290940132	26.128749123033185	26.715787376959888	25.14717059060561
88-89	22.19156451118878	26.224604892173375	26.66372780602657	24.920102790611278
90-91	22.076176822380027	26.028830742018354	26.62994432291623	25.265048112685378
92-93	21.978798548458485	26.082801328826687	26.660494862002853	25.27790526071198
94-95	22.213433331013174	26.202805295314946	26.473278337790855	25.110483035881025
96-97	22.1593123160094	26.211578474839737	26.315792828245634	25.313316380905228
98-99	22.123219385464786	26.293632079403206	26.57314841550319	25.010000119628824
100-101	22.195903699975624	26.375733077898563	26.48539773347182	24.94296548865399
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	52.0
2	37.5
3	38.5
4	65.5
5	92.5
6	104.0
7	128.0
8	159.0
9	180.5
10	209.0
11	240.5
12	282.5
13	328.0
14	370.0
15	441.5
16	513.5
17	607.0
18	768.0
19	931.5
20	1245.0
21	1713.5
22	2405.0
23	3579.0
24	5588.5
25	8101.5
26	11376.0
27	16717.5
28	24440.0
29	35286.5
30	50371.5
31	69490.5
32	96012.0
33	131418.5
34	176613.5
35	241127.5
36	322893.0
37	421516.5
38	558010.5
39	736748.5
40	936454.0
41	1150833.0
42	1375238.5
43	1589327.5
44	1768065.5
45	1882389.5
46	1948982.5
47	1958607.5
48	1886364.0
49	1765124.0
50	1621191.0
51	1457421.5
52	1309288.0
53	1175188.0
54	1019991.5
55	865056.5
56	730428.0
57	596174.5
58	468391.0
59	377500.0
60	309799.0
61	259671.5
62	219399.0
63	179537.0
64	156918.5
65	142007.5
66	124342.0
67	105653.5
68	76108.5
69	51583.0
70	40705.5
71	32160.5
72	25292.0
73	23422.5
74	19962.5
75	13835.0
76	8418.5
77	2937.0
78	423.0
79	120.5
80	44.0
81	17.5
82	7.5
83	4.0
84	1.0
85	0.5
86	0.5
87	0.5
88	0.5
89	1.0
90	1.0
91	0.0
92	0.5
93	1.0
94	0.5
95	0.5
96	0.5
97	0.0
98	0.0
99	0.5
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.024259156033701858
2	0.0
3	0.002320668389103789
4	0.0
5	0.0
6	0.0
7	0.0
8	8.171367567266861E-4
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0024906328345029394
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	5.801670972759472E-4
26-27	0.0
28-29	6.89663422677323E-4
30-31	0.0
32-33	8.171367567266861E-6
34-35	7.681085513230849E-5
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	0.0
50-51	0.0
52-53	0.0
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0
70-71	0.0
72-73	4.249111134978768E-5
74-75	6.01412652950841E-4
76-77	0.0
78-79	0.0015378513761596232
80-81	0.0
82-83	0.0
84-85	0.0
86-87	0.0017846266766910826
88-89	3.595401729597419E-5
90-91	0.0
92-93	4.0856837836334304E-4
94-95	0.0014953602648098356
96-97	0.0
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.0594634E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.325645223831994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.95814471012664	19.408788599162698
2	15.987449965748043	10.016343713215765
3	6.709758376419783	6.3056253131208315
4	3.7842454532184213	4.741757220296782
5	2.376466508294505	3.7222173362576223
6	1.6460279828771889	3.0937733172066375
7	1.2013839255800918	2.6343888641235575
8	0.8749899197087969	2.192769903938161
9	0.6964156229295659	1.9634101859002988
>10	4.250612838952825	24.700459994113587
>50	0.33017954450918696	7.074677151333708
>100	0.17029647433197095	9.702604567121133
>500	0.009661702365318992	2.0262530121804962
>1k	0.004301311816177325	2.2970935182211543
>5k	6.566312144080766E-5	0.11983730380766139
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5759658376694424E-5	0.0	3.2685470269067444E-6	0.0	3.2685470269067444E-6
2	6.537094053813489E-5	3.2685470269067444E-6	6.537094053813489E-6	0.0	3.2685470269067444E-6
3	9.805641080720234E-5	3.2685470269067444E-6	9.805641080720233E-6	0.0	9.805641080720233E-6
4	1.53621710264617E-4	3.2685470269067444E-6	9.805641080720233E-6	0.0	9.805641080720233E-6
5	1.895757275605912E-4	6.537094053813489E-6	9.805641080720233E-6	0.0	9.805641080720233E-6
6	2.516781210718193E-4	6.537094053813489E-6	2.94169232421607E-5	0.0	1.3074188107626977E-5
7	2.8763213836779353E-4	6.537094053813489E-6	4.5759658376694424E-5	0.0	1.3074188107626977E-5
8	3.07243420529234E-4	6.537094053813489E-6	4.5759658376694424E-5	0.0	1.3074188107626977E-5
9	3.235861556637677E-4	6.537094053813489E-6	4.902820540360117E-5	0.0	1.3074188107626977E-5
10-11	3.5790589944628855E-4	1.9611282161440465E-5	5.2296752430507917E-5	0.0	1.9611282161440465E-5
12-13	4.134711989037032E-4	3.2685470269067444E-5	5.5565299457414656E-5	0.0	2.9416923242160698E-5
14-15	4.968191480898252E-4	3.758829080942756E-5	5.5565299457414656E-5	0.0	3.758829080942756E-5
16-17	5.507501740337865E-4	4.412538486324105E-5	5.5565299457414656E-5	0.0	4.5759658376694424E-5
18-19	6.046811999777478E-4	4.902820540360117E-5	5.5565299457414656E-5	0.0	4.5759658376694424E-5
20-21	7.027376107849501E-4	4.902820540360117E-5	5.5565299457414656E-5	0.0	4.902820540360117E-5
22-23	7.828170129441653E-4	4.902820540360117E-5	5.5565299457414656E-5	0.0	5.393102594396128E-5
24-25	8.90679064832088E-4	5.2296752430507917E-5	5.5565299457414656E-5	0.0	5.5565299457414656E-5
26-27	0.0010132495783410907	9.642213729374897E-5	5.5565299457414656E-5	0.0	5.88338464843214E-5
28-29	0.0011962882118478685	1.0132495783410907E-4	6.537094053813489E-5	0.0	5.88338464843214E-5
30-31	0.0015100687264309161	1.0132495783410907E-4	6.700521405158826E-5	0.0	6.210239351122814E-5
32-33	0.001905562916686632	1.0786205188792257E-4	6.863948756504164E-5	0.0	6.700521405158826E-5
34-35	0.002436701808558978	1.1113059891482931E-4	6.863948756504164E-5	0.0	7.027376107849501E-5
36-37	0.003025040273402192	1.1113059891482931E-4	6.863948756504164E-5	0.0	7.190803459194838E-5
38-39	0.003959844723097522	1.1439914594173606E-4	6.863948756504164E-5	0.0	7.681085513230849E-5
40-41	0.005270532080887125	1.1603341945518943E-4	6.863948756504164E-5	3.2685470269067444E-6	9.151931675338885E-5
42-43	0.0069260511500153915	1.2420478702245628E-4	6.863948756504164E-5	3.2685470269067444E-6	9.151931675338885E-5
44-45	0.008826711246161663	1.4381606918389674E-4	6.863948756504164E-5	3.2685470269067444E-6	9.315359026684222E-5
46-47	0.010843404761763126	1.765015394529642E-4	6.863948756504164E-5	3.2685470269067444E-6	9.969068432065571E-5
48-49	0.013623304008147312	1.7977008647987095E-4	6.863948756504164E-5	3.2685470269067444E-6	1.0622777837446919E-4
50-51	0.01748018949989727	1.7977008647987095E-4	6.863948756504164E-5	3.2685470269067444E-6	1.0786205188792257E-4
52-53	0.022250633885667663	1.830386335067777E-4	7.190803459194838E-5	3.2685470269067444E-6	1.0786205188792257E-4
54-55	0.027789186822761144	1.895757275605912E-4	7.190803459194838E-5	3.2685470269067444E-6	1.1439914594173606E-4
56-57	0.03368727993281436	2.2879829188347212E-4	8.007940215921523E-5	3.2685470269067444E-6	1.2747333404936303E-4
58-59	0.040971236982276046	2.3696965945073898E-4	1.0295923134756245E-4	3.2685470269067444E-6	1.3727897513008327E-4
60-61	0.05022939643598939	2.451410270180058E-4	1.0459350486101582E-4	3.2685470269067444E-6	1.3727897513008327E-4
62-63	0.062054999579337994	2.4840957404491257E-4	1.0459350486101582E-4	6.537094053813489E-6	1.3727897513008327E-4
64-65	0.0764464121388084	2.4840957404491257E-4	1.0459350486101582E-4	6.537094053813489E-6	1.3727897513008327E-4
66-67	0.09330721197710684	2.4840957404491257E-4	1.0459350486101582E-4	6.537094053813489E-6	1.3727897513008327E-4
68-69	0.1131570980715115	2.4840957404491257E-4	1.0459350486101582E-4	6.537094053813489E-6	1.3891324864353664E-4
70-71	0.13799642120248928	2.516781210718193E-4	1.0459350486101582E-4	6.537094053813489E-6	1.4054752215699002E-4
72-73	0.16904108086404956	2.516781210718193E-4	1.0786205188792257E-4	6.537094053813489E-6	1.4381606918389676E-4
74-75	0.2074432398831769	2.5984948863908616E-4	1.0786205188792257E-4	6.537094053813489E-6	1.4871888972425688E-4
76-77	0.2543501582663156	3.252204291772211E-4	1.0786205188792257E-4	6.537094053813489E-6	1.5035316323771026E-4
78-79	0.31150724012583386	3.3829461728484807E-4	1.0786205188792257E-4	6.537094053813489E-6	1.5198743675116363E-4
80-81	0.3795257037557632	3.4319743782520816E-4	1.0786205188792257E-4	6.537094053813489E-6	1.53621710264617E-4
82-83	0.4624274962727124	3.4319743782520816E-4	1.0786205188792257E-4	6.537094053813489E-6	1.53621710264617E-4
84-85	0.5628160153836127	3.464659848521149E-4	1.0786205188792257E-4	6.537094053813489E-6	1.5689025729152372E-4
86-87	0.686912940354181	3.4973453187902166E-4	1.0786205188792257E-4	6.537094053813489E-6	1.5689025729152372E-4
88-89	0.8359652218751825	3.7424863458082223E-4	1.0949632540137594E-4	6.537094053813489E-6	1.5689025729152372E-4
90	0.9648325912315212	3.8568854917499587E-4	1.2093623999554955E-4	6.537094053813489E-6	1.5689025729152372E-4
>>END_MODULE
