##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654132_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	30971893
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.623174469833017	32.0	32.0	32.0	32.0	32.0
2	31.68117576797776	32.0	32.0	32.0	32.0	32.0
3	35.70151640392145	37.0	37.0	37.0	32.0	37.0
4	36.345867558046905	37.0	37.0	37.0	37.0	37.0
5	36.426771524749874	37.0	37.0	37.0	37.0	37.0
6	40.073332747210515	41.0	41.0	41.0	37.0	41.0
7	40.088462497271315	41.0	41.0	41.0	37.0	41.0
8	40.22490407673822	41.0	41.0	41.0	37.0	41.0
9	40.2035555269418	41.0	41.0	41.0	37.0	41.0
10-11	40.27283522837949	41.0	41.0	41.0	41.0	41.0
12-13	40.23423272836439	41.0	41.0	41.0	41.0	41.0
14-15	40.26595817052577	41.0	41.0	41.0	41.0	41.0
16-17	40.26654318481599	41.0	41.0	41.0	41.0	41.0
18-19	40.260203775726595	41.0	41.0	41.0	41.0	41.0
20-21	40.191449130991124	41.0	41.0	41.0	41.0	41.0
22-23	40.14805020151658	41.0	41.0	41.0	39.0	41.0
24-25	40.184192761482166	41.0	41.0	41.0	41.0	41.0
26-27	40.158911452393305	41.0	41.0	41.0	41.0	41.0
28-29	40.076709405524554	41.0	41.0	41.0	39.0	41.0
30-31	40.08916997743728	41.0	41.0	41.0	37.0	41.0
32-33	40.10098891921136	41.0	41.0	41.0	37.0	41.0
34-35	40.0919777489868	41.0	41.0	41.0	37.0	41.0
36-37	40.07208535816652	41.0	41.0	41.0	37.0	41.0
38-39	40.046567189160825	41.0	41.0	41.0	37.0	41.0
40-41	40.03725560139317	41.0	41.0	41.0	37.0	41.0
42-43	39.97722820816926	41.0	41.0	41.0	37.0	41.0
44-45	39.9979283959169	41.0	41.0	41.0	37.0	41.0
46-47	39.98324902194386	41.0	41.0	41.0	37.0	41.0
48-49	39.96331213917083	41.0	41.0	41.0	37.0	41.0
50-51	39.89070727772435	41.0	41.0	41.0	37.0	41.0
52-53	39.91134691056824	41.0	41.0	41.0	37.0	41.0
54-55	39.857146494080936	41.0	41.0	41.0	37.0	41.0
56-57	39.85602134490133	41.0	41.0	41.0	37.0	41.0
58-59	39.84907451410865	41.0	41.0	41.0	37.0	41.0
60-61	39.77177307179771	41.0	41.0	41.0	37.0	41.0
62-63	39.73361164911683	41.0	41.0	41.0	37.0	41.0
64-65	39.720902819856704	41.0	41.0	41.0	37.0	41.0
66-67	39.685380273656506	41.0	41.0	41.0	37.0	41.0
68-69	39.600706324279244	41.0	41.0	41.0	37.0	41.0
70-71	39.61539632078672	41.0	41.0	41.0	37.0	41.0
72-73	39.52133214782836	41.0	41.0	41.0	37.0	41.0
74-75	39.559008776118404	41.0	41.0	41.0	37.0	41.0
76-77	38.965468336081365	41.0	39.0	41.0	34.5	41.0
78-79	39.30564591256983	41.0	41.0	41.0	37.0	41.0
80-81	39.53652053169627	41.0	41.0	41.0	37.0	41.0
82-83	39.67933419503936	41.0	41.0	41.0	37.0	41.0
84-85	39.6372325546908	41.0	41.0	41.0	37.0	41.0
86-87	39.650990657884556	41.0	41.0	41.0	37.0	41.0
88-89	39.58359658545895	41.0	41.0	41.0	37.0	41.0
90-91	39.596770885137694	41.0	41.0	41.0	37.0	41.0
92-93	39.54589114071911	41.0	41.0	41.0	37.0	41.0
94-95	39.39603452717598	41.0	41.0	41.0	37.0	41.0
96-97	39.351478677780534	41.0	41.0	41.0	37.0	41.0
98-99	39.39728388574763	41.0	41.0	41.0	37.0	41.0
100-101	38.838749459065994	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	2.0
15	3.0
16	7.0
17	33.0
18	61.0
19	192.0
20	559.0
21	1627.0
22	4129.0
23	8756.0
24	16402.0
25	28260.0
26	46136.0
27	71380.0
28	103461.0
29	142022.0
30	185161.0
31	235230.0
32	289770.0
33	355457.0
34	423385.0
35	515788.0
36	654396.0
37	891337.0
38	1405818.0
39	3203586.0
40	2.2388934E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.105143613329478	11.13787385421322	6.9437502549548284	51.81323227750247
2	21.506444568951597	13.109004993656667	35.331469729667475	30.053080707724256
3	19.92784231819476	14.52077533652851	21.13677068431045	44.41461166096629
4	23.185113256631574	21.784079158266703	20.451232941202154	34.57957464389957
5	24.61683242932552	27.144811587719225	24.22406986876779	24.014286114187467
6	20.374318095442213	31.291419610677334	25.411523925902753	22.9227383679777
7	15.75134913452013	26.00232087848166	39.35240251540324	18.89392747159497
8	18.064720809929184	24.52342192968315	32.85384913347079	24.55800812691688
9	18.317638937002844	22.465989429542113	35.13733946168344	24.0790321717716
10-11	20.680360941622304	31.29254175029981	25.510443691596148	22.516653616481744
12-13	21.286802869081114	25.31722392281558	27.682039477770115	25.713933730333196
14-15	20.8813649201229	26.09456741956328	27.473406291310642	25.55066136900318
16-17	21.509159511697412	26.365336642552524	26.782915211525737	25.342588634224327
18-19	21.226234406982645	26.53225946016315	26.66669271180086	25.574813421053342
20-21	21.551824075967225	26.534014717108178	26.878131227695924	25.03602997922867
22-23	21.379148507325656	26.785164536116667	26.896951374589857	24.93873558196782
24-25	21.317337970225747	26.435309046742944	26.784425588458955	25.462927394572354
26-27	21.20384924486211	26.55190788628903	27.049139359999725	25.195103508849137
28-29	21.326181692360695	26.717674755918036	26.80575966807504	25.150383883646228
30-31	21.315502090879622	26.336940399477683	26.888417830966937	25.459139678675758
32-33	21.44229727633193	26.370550979722022	26.915964255277107	25.27118748866894
34-35	21.549889287672176	26.503853888075728	26.622137641807235	25.324119182444853
36-37	21.396660684605745	26.267603229295844	26.569014829392607	25.766721256705804
38-39	21.468044997920202	26.463407930293766	26.886745687454454	25.18180138433158
40-41	21.59522862581709	26.625631371224102	26.69086797912196	25.088272023836854
42-43	21.61162541792328	26.226343349436217	26.641342200168395	25.52068903247212
44-45	21.6015656651212	26.25777302811973	26.890239692182895	25.250421614576172
46-47	21.615332384298895	26.420100136155984	26.66508888883866	25.29947859070646
48-49	21.53925568718369	26.181556418116557	26.694674472819518	25.584513421880235
50-51	21.615764478518646	26.208368531923988	26.844834495699388	25.33103249385798
52-53	21.960637020152433	26.360850788164612	26.501999732467112	25.176512459215843
54-55	21.80420645165415	26.12224245094427	26.501817138282817	25.57173395911876
56-57	21.7117338344302	26.098277272612876	26.90900698521149	25.28098190774543
58-59	21.723177837202375	26.2842668421648	26.72572540897442	25.2668299116584
60-61	21.60853437610317	26.053606415098297	26.700638674676146	25.63722053412239
62-63	21.792016777589378	26.04219229617376	26.91123953872672	25.254551387510148
64-65	21.91975386173159	26.222938981073067	26.626560602572518	25.230746554622822
66-67	21.759709467478665	26.032647486867344	26.632632647306053	25.575010398347942
68-69	21.808608813816942	26.08780848791984	26.815381007839896	25.288201690423325
70-71	21.94448717952237	26.282062697436935	26.606219991075335	25.16723013196536
72-73	21.914496466271654	26.109629058211535	26.50843856414759	25.467435911369225
74-75	21.87412646478864	26.11447453586388	26.813255887700983	25.1981431116465
76-77	21.868511556591002	26.24096789950811	26.648501917528904	25.24201862637198
78-79	21.785894220863096	25.984026399000165	26.70585670505339	25.524222675083358
80-81	21.88149021704043	26.053450448376026	26.8087700792597	25.256289255323843
82-83	21.96895001331283	26.28886624484511	26.518380971391593	25.223802770450465
84-85	22.023876846858816	25.978770396145272	26.50012293423269	25.497229822763224
86-87	22.013801160507736	26.002871117994747	26.753733942796732	25.22959377870079
88-89	22.121021498370325	26.200634344053743	26.62204785018234	25.056296307393595
90-91	22.051431222187254	25.952366630313524	26.587261573151842	25.408940574347376
92-93	22.072427009677533	25.9809122792018	26.708547707186092	25.238113003934576
94-95	22.265581115790965	26.154283411226274	26.407487552354482	25.17264792062828
96-97	22.176435244622915	26.086067908672494	26.277679137125997	25.459817709578598
98-99	22.207660043089543	26.209620343471336	26.537882028364912	25.04483758507421
100-101	22.227633160272838	26.365184325647185	26.401545484895838	25.00563702918414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	38.5
2	19.5
3	21.0
4	40.5
5	61.5
6	74.5
7	97.0
8	114.0
9	125.5
10	166.5
11	198.0
12	202.5
13	247.5
14	312.0
15	375.0
16	451.0
17	536.5
18	668.5
19	894.5
20	1255.0
21	1721.5
22	2412.5
23	3406.5
24	5211.0
25	7843.5
26	11272.5
27	16718.0
28	24789.0
29	36096.0
30	50927.0
31	70283.0
32	97672.0
33	134539.5
34	182711.5
35	246303.5
36	323045.0
37	413249.0
38	535816.0
39	706456.0
40	908446.5
41	1132031.0
42	1371783.5
43	1602817.0
44	1801336.0
45	1943959.5
46	2024708.0
47	2036406.5
48	1965938.5
49	1833414.5
50	1677899.5
51	1503667.5
52	1332427.5
53	1176680.5
54	1008797.0
55	842244.5
56	696328.0
57	574708.5
58	472659.5
59	385804.0
60	315458.5
61	265982.0
62	226504.0
63	186648.5
64	160766.5
65	140597.0
66	118831.0
67	98875.5
68	71074.0
69	49282.0
70	40943.5
71	32333.5
72	24833.5
73	23188.5
74	20337.5
75	14904.5
76	9167.0
77	3014.0
78	452.5
79	144.0
80	47.5
81	18.0
82	8.0
83	5.0
84	4.0
85	3.0
86	1.0
87	0.0
88	0.0
89	1.0
90	1.0
91	0.5
92	0.5
93	0.0
94	0.0
95	1.0
96	1.0
97	0.5
98	1.0
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033985652733593004
2	0.0
3	0.0
4	0.019307828552810772
5	0.0
6	0.0
7	0.0
8	0.0
9	0.002776711129668438
10-11	0.003446673408047742
12-13	0.0016579548431217943
14-15	0.0
16-17	7.635955606588206E-4
18-19	5.488847581902727E-5
20-21	3.7291876218221466E-4
22-23	0.0
24-25	1.6143669358537433E-6
26-27	0.0
28-29	2.3731193957050024E-4
30-31	0.0
32-33	0.001963070193998152
34-35	5.940870323941776E-4
36-37	4.052061008992896E-4
38-39	0.004360405093740961
40-41	6.134594356244225E-5
42-43	0.0
44-45	0.0025006543836374484
46-47	2.0341023391757165E-4
48-49	5.504991251261264E-4
50-51	2.9058604845367376E-5
52-53	0.0
54-55	8.217127703495554E-4
56-57	3.3417395572172485E-4
58-59	7.861966977607731E-4
60-61	6.941777824171096E-5
62-63	3.2771648797830986E-4
64-65	0.002205225234376213
66-67	0.0020244161375605942
68-69	0.008423766671284832
70-71	0.008615876336651427
72-73	6.909490485454021E-4
74-75	0.0012995653833622633
76-77	0.0
78-79	0.0013463820245020219
80-81	0.008672379179406308
82-83	0.005705172751307129
84-85	1.5497922584195936E-4
86-87	0.010075264046663212
88-89	1.8403783068732672E-4
90-91	0.0022794861134254854
92-93	6.505898751490585E-4
94-95	0.014174141696795867
96-97	0.0010525672421766406
98-99	3.5193199201611605E-4
100-101	0.015444648475312761
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.0971893E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.84744338809976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.85999842997402	21.276502382345857
2	15.840149490880712	10.722971263032443
3	6.765534624120484	6.869881505404408
4	3.7104671499590944	5.023593072065773
5	2.339306806723806	3.958977735399024
6	1.5422769087469685	3.132127821455191
7	1.1450416974767752	2.7129713822651165
8	0.8231977236005859	2.2290510677426743
9	0.6709873841191419	2.0440086748291626
>10	3.887310512271153	24.08806804532782
>50	0.2691604005362874	6.242526290779017
>100	0.13628213814730367	8.357538084694383
>500	0.007394626999024259	1.6838547101229344
>1k	0.0028620768254293467	1.5988973397241382
>5k	3.0029619379422354E-5	0.05903062481206172
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.84310080756123E-5	3.2287338717074866E-6	0.0	0.0	6.457467743414973E-6
2	5.488847581902727E-5	3.2287338717074866E-6	3.2287338717074866E-6	0.0	2.905860484536738E-5
3	7.426087904927219E-5	3.2287338717074866E-6	3.2287338717074866E-6	0.0	3.2287338717074866E-5
4	1.1300568550976203E-4	3.2287338717074866E-6	9.68620161512246E-6	0.0	3.551607258878235E-5
5	1.3883555648342192E-4	3.2287338717074866E-6	9.68620161512246E-6	0.0	3.551607258878235E-5
6	1.7435162907220427E-4	3.2287338717074866E-6	2.905860484536738E-5	0.0	3.551607258878235E-5
7	2.098677016609866E-4	3.2287338717074866E-6	2.905860484536738E-5	0.0	3.551607258878235E-5
8	2.4538377424976896E-4	9.68620161512246E-6	2.905860484536738E-5	0.0	3.551607258878235E-5
9	2.5829870973659893E-4	1.6143669358537433E-5	2.905860484536738E-5	0.0	3.874480646048984E-5
10-11	2.922004153895275E-4	2.5829870973659893E-5	3.067297178122112E-5	0.0	4.84310080756123E-5
12-13	3.244877541066024E-4	4.84310080756123E-5	3.2287338717074866E-5	0.0	6.296031049829599E-5
14-15	3.7937622992562965E-4	5.488847581902727E-5	3.2287338717074866E-5	0.0	6.780341130585721E-5
16-17	4.326503388088032E-4	6.134594356244224E-5	3.2287338717074866E-5	0.0	6.780341130585721E-5
18-19	5.133686856014903E-4	7.264651211341845E-5	3.2287338717074866E-5	0.0	6.780341130585721E-5
20-21	6.247600041753987E-4	7.426087904927219E-5	3.551607258878235E-5	0.0	6.780341130585721E-5
22-23	7.16778919519062E-4	7.426087904927219E-5	3.551607258878235E-5	0.0	7.10321451775647E-5
24-25	8.168696695419941E-4	8.23327137285409E-5	3.551607258878235E-5	0.0	7.10321451775647E-5
26-27	9.750776292556609E-4	1.1139131857390829E-4	3.551607258878235E-5	0.0	7.587524598512593E-5
28-29	0.0011833309639807939	1.1623441938146952E-4	4.1973540332197326E-5	0.0	7.910397985683341E-5
30-31	0.0015045899842156887	1.1623441938146952E-4	5.004537501146604E-5	0.0	8.55614476002484E-5
32-33	0.001904952984307417	1.19463153253177E-4	5.327410888317353E-5	0.0	8.717581453610213E-5
34-35	0.002413478569101346	1.19463153253177E-4	5.650284275488101E-5	0.0	8.717581453610213E-5
36-37	0.0030479247748918673	1.19463153253177E-4	5.811720969073476E-5	0.0	9.36332822795171E-5
38-39	0.003981028863815331	1.19463153253177E-4	5.811720969073476E-5	0.0	9.68620161512246E-5
40-41	0.005224091404422713	1.2592062099659197E-4	5.811720969073476E-5	0.0	9.68620161512246E-5
42-43	0.006804556634623528	1.6143669358537435E-4	5.811720969073476E-5	0.0	9.68620161512246E-5
44-45	0.008635248739881673	1.8403783068732672E-4	6.780341130585721E-5	0.0	1.049338508304933E-4
46-47	0.010685494748415927	2.0179586698171792E-4	6.780341130585721E-5	0.0	1.0654821776634705E-4
48-49	0.013505793785352415	2.0341023391757165E-4	6.780341130585721E-5	0.0	1.1784878631732325E-4
50-51	0.017105832052306265	2.0825333472513288E-4	6.780341130585721E-5	0.0	1.3237808874000696E-4
52-53	0.021642203142055283	2.2116827021196283E-4	6.780341130585721E-5	0.0	1.3560682261171443E-4
54-55	0.02690826808681019	2.243970040836703E-4	6.941777824171096E-5	0.0	1.3722118954756816E-4
56-57	0.03289111195108417	2.792854799026976E-4	8.717581453610213E-5	1.6143669358537433E-6	1.420642903551294E-4
58-59	0.04002499944062186	2.9704351619708874E-4	1.1139131857390829E-4	3.2287338717074866E-6	1.4367865729098315E-4
60-61	0.04912680022496527	3.1480155249147994E-4	1.1784878631732325E-4	3.2287338717074866E-6	1.4690739116269064E-4
62-63	0.06101984144139979	3.2287338717074864E-4	1.2269188712488448E-4	3.2287338717074866E-6	1.4852175809854437E-4
64-65	0.0752553290817581	3.2610212104245616E-4	1.2269188712488448E-4	3.2287338717074866E-6	1.5175049197025187E-4
66-67	0.09219972444047898	3.2610212104245616E-4	1.2269188712488448E-4	3.2287338717074866E-6	1.5175049197025187E-4
68-69	0.11259563630805518	3.277164879783099E-4	1.2269188712488448E-4	3.2287338717074866E-6	1.5175049197025187E-4
70-71	0.13759087957587868	3.293308549141636E-4	1.2269188712488448E-4	3.2287338717074866E-6	1.5175049197025187E-4
72-73	0.16869973042978031	3.293308549141636E-4	1.2592062099659197E-4	3.2287338717074866E-6	1.5175049197025187E-4
74-75	0.20869405689862097	3.470888912085548E-4	1.2914935486829947E-4	3.2287338717074866E-6	1.5497922584195936E-4
76-77	0.257447938361404	4.358790726805107E-4	1.2914935486829947E-4	3.2287338717074866E-6	1.6143669358537432E-4
78-79	0.31626901203617097	4.455652742956331E-4	1.2914935486829947E-4	3.2287338717074866E-6	1.7435162907220427E-4
80-81	0.3866537960724583	4.455652742956331E-4	1.3237808874000696E-4	3.2287338717074866E-6	1.7596599600805803E-4
82-83	0.47248968605180186	4.455652742956331E-4	1.339924556758607E-4	3.2287338717074866E-6	1.8403783068732672E-4
84-85	0.578971714773779	4.5686584284660933E-4	1.3560682261171443E-4	3.2287338717074866E-6	1.8888093149488795E-4
86-87	0.7109171531749771	4.7462387914100053E-4	1.420642903551294E-4	3.2287338717074866E-6	1.904952984307417E-4
88-89	0.8692316611064101	5.101399517297828E-4	1.452930242268369E-4	6.457467743414973E-6	1.9372403230244917E-4
90	1.005363152972277	5.359698227034428E-4	1.452930242268369E-4	6.457467743414973E-6	2.0018150004586416E-4
>>END_MODULE
