##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654107_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29814926
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.257335872643118	32.0	32.0	32.0	32.0	32.0
2	31.27254791777783	32.0	32.0	32.0	32.0	32.0
3	34.919792422090865	37.0	32.0	37.0	32.0	37.0
4	35.69772928498967	37.0	37.0	37.0	32.0	37.0
5	35.98445724802403	37.0	37.0	37.0	37.0	37.0
6	39.34353511392247	41.0	41.0	41.0	37.0	41.0
7	39.352920748486845	41.0	41.0	41.0	37.0	41.0
8	39.44910820841883	41.0	41.0	41.0	37.0	41.0
9	39.50834749682089	41.0	41.0	41.0	37.0	41.0
10-11	39.52939898291211	41.0	41.0	41.0	37.0	41.0
12-13	39.51277113684602	41.0	41.0	41.0	37.0	41.0
14-15	39.436738430945624	41.0	41.0	41.0	37.0	41.0
16-17	39.377773350837764	41.0	41.0	41.0	37.0	41.0
18-19	39.15862568298844	41.0	41.0	41.0	37.0	41.0
20-21	39.10399509292762	41.0	41.0	41.0	37.0	41.0
22-23	39.02420093211032	41.0	41.0	41.0	37.0	41.0
24-25	38.94241246817114	41.0	41.0	41.0	37.0	41.0
26-27	38.89592048626919	41.0	41.0	41.0	37.0	41.0
28-29	39.005264762354265	41.0	41.0	41.0	37.0	41.0
30-31	39.11175947577398	41.0	41.0	41.0	37.0	41.0
32-33	39.14630933512966	41.0	41.0	41.0	37.0	41.0
34-35	39.151947316589016	41.0	41.0	41.0	37.0	41.0
36-37	39.15347001028948	41.0	41.0	41.0	37.0	41.0
38-39	39.1283790206288	41.0	41.0	41.0	37.0	41.0
40-41	39.130282412909565	41.0	41.0	41.0	37.0	41.0
42-43	39.09905223309963	41.0	41.0	41.0	37.0	41.0
44-45	39.0903066806203	41.0	41.0	41.0	37.0	41.0
46-47	39.027667702411875	41.0	41.0	41.0	37.0	41.0
48-49	38.99454342096975	41.0	41.0	41.0	37.0	41.0
50-51	38.98688170482127	41.0	41.0	41.0	37.0	41.0
52-53	38.98497393553819	41.0	41.0	41.0	37.0	41.0
54-55	38.97586068132452	41.0	41.0	41.0	37.0	41.0
56-57	38.9187714234139	41.0	41.0	41.0	32.0	41.0
58-59	38.873980351318	41.0	41.0	41.0	32.0	41.0
60-61	38.816430971520774	41.0	41.0	41.0	32.0	41.0
62-63	38.75004132158503	41.0	41.0	41.0	32.0	41.0
64-65	38.75869103280686	41.0	41.0	41.0	32.0	41.0
66-67	38.74230284857994	41.0	41.0	41.0	32.0	41.0
68-69	38.67462052396172	41.0	41.0	41.0	32.0	41.0
70-71	38.5968797306423	41.0	41.0	41.0	32.0	41.0
72-73	38.6044526657554	41.0	41.0	41.0	32.0	41.0
74-75	38.499052253223766	41.0	41.0	41.0	32.0	41.0
76-77	37.692530496302425	41.0	39.0	41.0	29.5	41.0
78-79	38.238665442268754	41.0	37.0	41.0	32.0	41.0
80-81	38.69560972581317	41.0	41.0	41.0	32.0	41.0
82-83	38.75508450029358	41.0	41.0	41.0	32.0	41.0
84-85	38.71387963532091	41.0	41.0	41.0	32.0	41.0
86-87	38.66382225801935	41.0	41.0	41.0	32.0	41.0
88-89	38.580638301768715	41.0	41.0	41.0	32.0	41.0
90-91	38.454773592260466	41.0	41.0	41.0	32.0	41.0
92-93	38.35413797438236	41.0	37.0	41.0	32.0	41.0
94-95	38.218277566746266	41.0	37.0	41.0	32.0	41.0
96-97	38.23856529108944	41.0	37.0	41.0	32.0	41.0
98-99	38.15791979493761	41.0	37.0	41.0	32.0	41.0
100-101	37.09718397422821	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	37.0
13	252.0
14	674.0
15	1733.0
16	5423.0
17	13832.0
18	24235.0
19	34169.0
20	44947.0
21	57635.0
22	72074.0
23	89315.0
24	107945.0
25	128153.0
26	150880.0
27	178070.0
28	208739.0
29	244532.0
30	287618.0
31	340098.0
32	402066.0
33	479829.0
34	585800.0
35	734742.0
36	957504.0
37	1341690.0
38	2173883.0
39	5198074.0
40	1.5950977E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	37.705957681287344	21.685473738477228	12.193568938841295	28.414999641394132
2	30.752656425762297	23.72749102136411	29.106954444558937	16.412898108314657
3	21.97980754866407	27.120243949412075	29.046860297733605	21.85308820419024
4	24.84361173320947	32.552405770671285	22.587890952219524	20.016091543899716
5	26.800685376766374	33.7643312012185	21.181188885723426	18.25379453629169
6	22.09995014181827	36.798101867325585	21.503406137235274	19.598541853620873
7	21.594663692943595	20.360976243912194	37.30315144837187	20.741208614772347
8	23.10730841970922	24.441257071029572	25.936750177654282	26.514684331606926
9	23.153010676598697	23.860226921240724	28.519319484475663	24.46744291768492
10-11	25.50548674848296	29.92628926867033	22.475111962377504	22.09311202046921
12-13	25.71342168339849	24.552375147452942	25.84967928723087	23.8845238819177
14-15	24.43903265096147	26.973754689178165	26.361116911710596	22.226095748149767
16-17	25.333592848242258	26.647699002373017	25.669134825402075	22.349573323982657
18-19	24.92919318330691	27.25012800635494	25.737419237599312	22.083259572738836
20-21	25.0427216079595	27.30804892551712	25.58100238067763	22.068227085845752
22-23	25.156165491676308	27.173639927424944	25.587598734734385	22.082595846164367
24-25	24.785364589027314	27.06784098477945	25.901205267383	22.24558915881024
26-27	24.907387457650433	27.120899628711282	25.750441794953975	22.22127111868431
28-29	25.223246119321026	26.92139241180537	25.70551274361028	22.149848725263315
30-31	24.741077671709366	27.100619480995686	25.97495495327794	22.183347894017004
32-33	24.849206129729676	27.184525317544168	25.772962442012208	22.193306110713944
34-35	25.181449385452105	26.938597801651426	25.633524966655962	22.246427846240504
36-37	24.860360210184655	26.852292036545723	25.95507364331543	22.332274109954188
38-39	24.907964297085133	27.064294980463043	25.693556213677148	22.334184508774673
40-41	25.217755026458892	26.819387376644837	25.662525541737047	22.300332055159217
42-43	24.894667206329586	26.908822373505604	25.946545364931957	22.249965055232856
44-45	24.975229669651057	26.944464433853998	25.824470347775087	22.25583554871986
46-47	25.208019969595096	26.762467899467534	25.717689186952867	22.3118229439845
48-49	24.76672001320163	26.834034921604406	26.023189391602976	22.376055673590983
50-51	25.01012507668645	26.893691206006565	25.788066372472883	22.308117344834102
52-53	25.18889661288809	26.847863951238587	25.79788770125041	22.165351734622917
54-55	24.89550963266119	26.861555923233865	26.040197942559228	22.202736501545715
56-57	24.7491677836093	26.95978421200018	26.031294989131794	22.259753015258728
58-59	25.17580441551766	26.62655171792138	25.892612671837206	22.30503119472375
60-61	24.913939078701723	26.72304636945938	26.05230179004972	22.31071276178918
62-63	24.899778165845973	26.883581570804772	25.92014852606061	22.296491737288644
64-65	25.132693613440033	26.79012866174001	25.90514233744148	22.172035387378482
66-67	24.9028566403159	26.839082699697563	26.161616564786748	22.09644409519979
68-69	25.04241427951938	26.974014384312834	25.91920580760434	22.064365528563442
70-71	25.283323027527032	26.885999446988063	25.65133355434134	22.179343971143563
72-73	24.851768160692586	26.92548698817753	26.135575686713203	22.087169164416682
74-75	24.828276399363375	27.04638995730036	25.980612898867715	22.144720744468547
76-77	25.272097333151567	26.830190734066246	25.804168002061655	22.093543930720532
78-79	24.904984046295166	26.848736157972763	26.169135482987233	22.07714431274484
80-81	24.94892998401011	26.913750008762527	26.013428517121273	22.123891490106093
82-83	25.24481628648832	26.779875583362312	25.942092234036156	22.03321589611321
84-85	25.00710092741793	26.902876234067563	26.099561232533507	21.990461605980997
86-87	24.96773260480338	27.13446613954366	25.944910948294826	21.952890307358132
88-89	25.276178783740733	26.990803197029567	25.770939361043528	21.962078658186172
90-91	24.994413871763424	26.97744076238861	26.095962807353608	21.932182558494357
92-93	25.117172854965332	27.081410498889046	25.857134443332175	21.94428220281345
94-95	25.466948400416157	26.939315700120652	25.747140415747005	21.846595483716186
96-97	25.14599829547206	26.949650683801885	26.069852237908204	21.834498782817857
98-99	25.278416342177344	27.145801521456093	25.856318171916946	21.719463964449616
100-101	25.706250636219153	26.955943532080788	25.68220888917436	21.6555969425257
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	56.0
1	46.5
2	24.0
3	22.0
4	42.0
5	48.5
6	51.5
7	59.0
8	80.0
9	109.0
10	132.5
11	162.5
12	188.0
13	230.5
14	303.0
15	403.0
16	491.0
17	588.5
18	762.5
19	976.5
20	1276.5
21	1666.0
22	2168.0
23	2861.0
24	3829.0
25	5232.0
26	7133.5
27	9911.5
28	14085.5
29	19307.0
30	26269.0
31	36509.5
32	52112.0
33	73887.0
34	108075.5
35	164124.0
36	246895.0
37	358880.0
38	514801.0
39	721274.5
40	962369.5
41	1221352.5
42	1490140.5
43	1724360.0
44	1899072.0
45	2014229.0
46	2058168.0
47	2047685.5
48	1967040.0
49	1804883.0
50	1597567.0
51	1401580.5
52	1223585.5
53	1040672.0
54	867408.0
55	712637.0
56	572821.0
57	458604.0
58	369187.0
59	309015.5
60	268699.0
61	228393.0
62	190171.0
63	159659.5
64	141795.5
65	124573.0
66	107892.0
67	96284.5
68	81310.5
69	64187.5
70	56461.5
71	51710.5
72	43276.5
73	32689.5
74	20646.0
75	12935.5
76	7521.0
77	2980.5
78	1346.5
79	782.0
80	490.5
81	344.0
82	271.5
83	223.5
84	176.0
85	161.5
86	153.0
87	137.5
88	129.5
89	117.0
90	98.0
91	79.5
92	68.5
93	71.5
94	66.5
95	58.0
96	62.5
97	62.0
98	71.5
99	138.0
100	650.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016904284786754126
2	0.0064866838844409675
3	7.378854470408546E-4
4	0.0017071986024717956
5	2.8173807977923543E-4
6	0.001210802938098857
7	0.0
8	0.023585502107233137
9	0.0
10-11	0.0
12-13	0.003947687141668573
14-15	0.0
16-17	0.001091735059144537
18-19	0.0
20-21	3.203093645109164E-4
22-23	1.6770123796383061E-6
24-25	2.1801160935297976E-5
26-27	0.005381532726259324
28-29	9.994993782644305E-4
30-31	1.6267020082491568E-4
32-33	4.578243796412575E-4
34-35	0.0
36-37	0.0
38-39	9.173257716621533E-4
40-41	0.0
42-43	6.204945804661732E-5
44-45	0.001769248060518413
46-47	0.0
48-49	0.0014455846712482197
50-51	0.0011303063438762183
52-53	6.842210508924288E-4
54-55	7.244693480037482E-4
56-57	0.00844375733147887
58-59	6.322336671236414E-4
60-61	0.0
62-63	0.00482141059146013
64-65	6.7080495185532246E-6
66-67	0.002488686371383246
68-69	2.85092104538512E-4
70-71	6.037244566697902E-5
72-73	1.9956447317695842E-4
74-75	5.165198129285982E-4
76-77	3.001852159552568E-4
78-79	2.1633459697334147E-4
80-81	0.0015596215130636248
82-83	0.005198738376878749
84-85	5.31612924345343E-4
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	5.031037138914918E-5
96-97	0.00467215648967232
98-99	1.6770123796383061E-6
100-101	4.0583699587247007E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.9814926E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.535883506500426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.863559488315914	22.694480102872543
2	15.008293387280927	10.666659308835914
3	6.9159832235105565	7.372967224907473
4	3.834601893472034	5.450638647209127
5	2.3593799466373593	4.192132546563919
6	1.5306737619403892	3.263630669446226
7	1.1310271830664667	2.813443515409471
8	0.8420130443292007	2.3937341963389005
9	0.6374835796003201	2.0388187999785488
>10	3.518971893519397	22.617148401220895
>50	0.23356489060909852	5.682194586011331
>100	0.11436488728130231	7.472613017569915
>500	0.007428907585629641	1.7899593352029401
>1k	0.0026242808871824496	1.4780698558600496
>5k	2.9631964299413453E-5	0.0735097925729231
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3416099037106447E-5	0.0	3.354024759276612E-6	0.0	0.0
2	3.0186222833489506E-5	3.354024759276612E-6	3.354024759276612E-6	1.3416099037106447E-5	0.0
3	4.0248297111319344E-5	3.354024759276612E-6	3.354024759276612E-6	1.3416099037106447E-5	3.354024759276612E-6
4	8.38506189819153E-5	3.354024759276612E-6	1.677012379638306E-5	1.3416099037106447E-5	3.354024759276612E-6
5	9.055866850046852E-5	3.354024759276612E-6	5.031037138914918E-5	1.3416099037106447E-5	3.354024759276612E-6
6	1.3416099037106448E-4	3.354024759276612E-6	6.372647042625563E-5	1.3416099037106447E-5	3.354024759276612E-6
7	1.5428513892672415E-4	6.708049518553224E-6	7.378854470408546E-5	1.3416099037106447E-5	3.354024759276612E-6
8	1.7776331224166042E-4	6.708049518553224E-6	9.055866850046852E-5	1.677012379638306E-5	3.354024759276612E-6
9	2.0794953507514996E-4	6.708049518553224E-6	9.391269325974513E-5	2.3478173314936283E-5	3.354024759276612E-6
10-11	2.565828940846608E-4	1.0062074277829836E-5	1.0732879229685158E-4	2.3478173314936283E-5	1.3416099037106447E-5
12-13	2.9683119119598017E-4	1.0062074277829836E-5	1.4086903988961771E-4	2.3478173314936283E-5	1.677012379638306E-5
14-15	3.5552662448332085E-4	1.677012379638306E-5	1.6267020082491568E-4	3.354024759276612E-5	1.677012379638306E-5
16-17	4.377002310855979E-4	2.0124148555659672E-5	2.800610673995971E-4	3.521725997240443E-5	1.677012379638306E-5
18-19	5.064577386507685E-4	2.0124148555659672E-5	3.052162530941717E-4	3.857128473168104E-5	1.677012379638306E-5
20-21	6.121095185679817E-4	2.0124148555659672E-5	3.488185749647676E-4	4.0248297111319344E-5	1.677012379638306E-5
22-23	7.529785584575995E-4	2.3478173314936283E-5	3.9409790921500194E-4	4.360232187059596E-5	1.677012379638306E-5
24-25	0.0010112384649218985	3.0186222833489506E-5	4.1254504539102324E-4	4.695634662987257E-5	1.677012379638306E-5
26-27	0.00133657886657173	3.0186222833489506E-5	4.2093010728921475E-4	4.695634662987257E-5	2.0124148555659672E-5
28-29	0.0017071986024717956	3.0186222833489506E-5	4.3602321870595955E-4	4.8633359009510874E-5	2.6832198074212895E-5
30-31	0.002201917254465096	3.0186222833489506E-5	4.527933425023426E-4	5.53414085280641E-5	2.85092104538512E-5
32-33	0.002703343955976949	3.521725997240443E-5	4.695634662987257E-4	5.7018420907702405E-5	3.0186222833489506E-5
34-35	0.003397627081147208	4.360232187059596E-5	5.399979862435345E-4	5.7018420907702405E-5	3.0186222833489506E-5
36-37	0.00430656779091117	4.527933425023426E-5	5.718612214566624E-4	5.7018420907702405E-5	3.0186222833489506E-5
38-39	0.00544525919668558	4.695634662987257E-5	5.852773204937688E-4	5.8695433287340705E-5	3.0186222833489506E-5
40-41	0.0069378002145636716	4.695634662987257E-5	6.238486052254498E-4	6.372647042625563E-5	3.0186222833489506E-5
42-43	0.008894873661601575	5.031037138914918E-5	6.657739147164075E-4	6.372647042625563E-5	3.1863235213127814E-5
44-45	0.011279585265447246	6.204945804661732E-5	6.858980632720672E-4	6.372647042625563E-5	3.354024759276612E-5
46-47	0.0137816877358676	6.372647042625563E-5	7.513015460779611E-4	6.372647042625563E-5	3.857128473168104E-5
48-49	0.017144097557042402	6.540348280589393E-5	7.965808803281954E-4	6.372647042625563E-5	4.0248297111319344E-5
50-51	0.021673707994445464	7.546555708372377E-5	8.150280165042167E-4	6.372647042625563E-5	4.0248297111319344E-5
52-53	0.0276421950535782	7.714256946336207E-5	8.569533259951744E-4	6.372647042625563E-5	4.0248297111319344E-5
54-55	0.03437204573306672	7.881958184300038E-5	8.98878635486132E-4	6.372647042625563E-5	4.192530949095765E-5
56-57	0.041833073810077545	8.552763136155361E-5	9.542200440141961E-4	6.372647042625563E-5	4.360232187059596E-5
58-59	0.05067596008791033	8.888165612083021E-5	9.944683411255155E-4	6.372647042625563E-5	4.360232187059596E-5
60-61	0.06207125920755262	9.223568088010683E-5	0.0010565177991721329	6.708049518553224E-5	4.360232187059596E-5
62-63	0.07731194771370554	9.391269325974513E-5	0.0011655236038486227	6.875750756517055E-5	4.360232187059596E-5
64-65	0.0957590838897269	9.391269325974513E-5	0.0012275730618952398	7.043451994480886E-5	4.360232187059596E-5
66-67	0.1173506182775701	1.1571385419504312E-4	0.0012409891609323465	7.211153232444716E-5	4.360232187059596E-5
68-69	0.1423012084618288	1.2409891609323464E-4	0.0012694983713861978	7.546555708372377E-5	4.527933425023426E-5
70-71	0.17291171542736683	1.2409891609323464E-4	0.001276206420904751	8.049659422263869E-5	5.031037138914918E-5
72-73	0.21251771679728468	1.2745294085251125E-4	0.001298007581840049	8.049659422263869E-5	6.037244566697901E-5
74-75	0.26411938771875537	1.2745294085251125E-4	0.001298007581840049	8.2173606602277E-5	6.037244566697901E-5
76-77	0.32741654297582357	1.3080696561178787E-4	0.0013030386189789639	8.38506189819153E-5	6.037244566697901E-5
78-79	0.4025567596578975	1.358380027507028E-4	0.0013080696561178786	8.38506189819153E-5	6.037244566697901E-5
80-81	0.493060086749838	1.442230646488943E-4	0.0013181317303957085	8.552763136155361E-5	6.708049518553224E-5
82-83	0.6042409764827188	1.5596215130636245E-4	0.0013449639284699214	8.720464374119192E-5	6.708049518553224E-5
84-85	0.7458193926089234	1.5763916368600076E-4	0.001360057039886666	8.720464374119192E-5	6.875750756517055E-5
86-87	0.9246794709468673	1.5931617606563907E-4	0.0013684421017848578	9.055866850046852E-5	7.211153232444716E-5
88-89	1.1393588567015058	1.6267020082491568E-4	0.001370119114164496	9.391269325974513E-5	7.378854470408546E-5
90	1.3201776854988672	1.7105526272310722E-4	0.0013717961265441343	9.391269325974513E-5	7.378854470408546E-5
>>END_MODULE
