##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654108_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	35030914
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.66679573361974	32.0	32.0	32.0	32.0	32.0
2	31.70128141675093	32.0	32.0	32.0	32.0	32.0
3	35.83257356630775	37.0	37.0	37.0	32.0	37.0
4	36.392091510943736	37.0	37.0	37.0	37.0	37.0
5	36.56515790595701	37.0	37.0	37.0	37.0	37.0
6	40.19213874922019	41.0	41.0	41.0	37.0	41.0
7	40.22515119074541	41.0	41.0	41.0	37.0	41.0
8	40.26051318558231	41.0	41.0	41.0	41.0	41.0
9	40.3062259237655	41.0	41.0	41.0	41.0	41.0
10-11	40.33225470508705	41.0	41.0	41.0	41.0	41.0
12-13	40.30964313406153	41.0	41.0	41.0	41.0	41.0
14-15	40.304799497952004	41.0	41.0	41.0	41.0	41.0
16-17	40.30298591409861	41.0	41.0	41.0	41.0	41.0
18-19	40.30336173929119	41.0	41.0	41.0	41.0	41.0
20-21	40.2738822486904	41.0	41.0	41.0	41.0	41.0
22-23	40.2804839748115	41.0	41.0	41.0	41.0	41.0
24-25	40.2682976099339	41.0	41.0	41.0	41.0	41.0
26-27	40.188203710585455	41.0	41.0	41.0	41.0	41.0
28-29	40.199464592902146	41.0	41.0	41.0	41.0	41.0
30-31	40.11400333431209	41.0	41.0	41.0	41.0	41.0
32-33	40.13994023964091	41.0	41.0	41.0	41.0	41.0
34-35	40.15282814202336	41.0	41.0	41.0	41.0	41.0
36-37	40.13987232819561	41.0	41.0	41.0	41.0	41.0
38-39	40.067018319875984	41.0	41.0	41.0	37.0	41.0
40-41	40.09341038032864	41.0	41.0	41.0	37.0	41.0
42-43	40.0769327914196	41.0	41.0	41.0	37.0	41.0
44-45	40.07598557091602	41.0	41.0	41.0	37.0	41.0
46-47	40.06347347945304	41.0	41.0	41.0	37.0	41.0
48-49	39.976577131273245	41.0	41.0	41.0	37.0	41.0
50-51	39.93725235088071	41.0	41.0	41.0	37.0	41.0
52-53	39.98255165423318	41.0	41.0	41.0	37.0	41.0
54-55	39.95286109291924	41.0	41.0	41.0	37.0	41.0
56-57	39.94218477999175	41.0	41.0	41.0	37.0	41.0
58-59	39.944743833974755	41.0	41.0	41.0	37.0	41.0
60-61	39.92588022396447	41.0	41.0	41.0	37.0	41.0
62-63	39.886523029344886	41.0	41.0	41.0	37.0	41.0
64-65	39.83642951765404	41.0	41.0	41.0	37.0	41.0
66-67	39.81255419142076	41.0	41.0	41.0	37.0	41.0
68-69	39.792678104259565	41.0	41.0	41.0	37.0	41.0
70-71	39.77083023012189	41.0	41.0	41.0	37.0	41.0
72-73	39.74265020604372	41.0	41.0	41.0	37.0	41.0
74-75	39.726243939852665	41.0	41.0	41.0	37.0	41.0
76-77	39.20112522613598	41.0	39.0	41.0	34.5	41.0
78-79	39.526475615223745	41.0	41.0	41.0	37.0	41.0
80-81	39.70744364534708	41.0	41.0	41.0	37.0	41.0
82-83	39.779459108603334	41.0	41.0	41.0	37.0	41.0
84-85	39.818612825802944	41.0	41.0	41.0	37.0	41.0
86-87	39.82781593994379	41.0	41.0	41.0	37.0	41.0
88-89	39.70995568371411	41.0	41.0	41.0	37.0	41.0
90-91	39.73321735196518	41.0	41.0	41.0	37.0	41.0
92-93	39.68987980159467	41.0	41.0	41.0	37.0	41.0
94-95	39.685703361893445	41.0	41.0	41.0	37.0	41.0
96-97	39.65222003627996	41.0	41.0	41.0	37.0	41.0
98-99	39.61268437072467	41.0	41.0	41.0	37.0	41.0
100-101	39.10626534037907	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	3.0
15	3.0
16	10.0
17	23.0
18	69.0
19	188.0
20	533.0
21	1462.0
22	3603.0
23	7777.0
24	15412.0
25	27971.0
26	46340.0
27	72564.0
28	105368.0
29	145065.0
30	188668.0
31	238717.0
32	293695.0
33	356640.0
34	428780.0
35	523120.0
36	661038.0
37	893894.0
38	1382068.0
39	3282705.0
40	2.6355195E7
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.889373135476006	11.845952553876055	7.8233036725197564	40.441370638128184
2	23.424935472708476	14.652446693226445	34.8439866570424	27.07863117702267
3	20.025923971460852	19.69305497107154	24.82106796120385	35.45995309626376
4	23.057220259796818	27.41214802445634	22.916150574889368	26.61448114085747
5	23.612289990492396	31.774135268066374	23.98836638975506	20.62520835168617
6	20.0653771123414	33.61226315705037	24.60954629959127	21.712813431016958
7	15.921931126318887	23.317641669298155	41.66961216027649	19.09081504410647
8	19.44087156846664	23.64184959604537	29.944342873839947	26.972935961648048
9	19.022012956898585	22.245925413193614	33.07984484789635	25.652216782011454
10-11	22.385713254578967	30.51219407026153	23.433855677965994	23.668236997193507
12-13	22.056207268814056	24.248572275333725	27.28388559887418	26.41133485697804
14-15	21.119243420254463	26.099496005157047	27.717398409873063	25.063862164715427
16-17	21.830034771727732	26.451668320417387	27.007000562453186	24.711296345401692
18-19	21.671389448453652	26.84805814137184	26.65004472426394	24.830507685910575
20-21	21.784447302745228	26.705994596658257	26.82662090974846	24.68293719084806
22-23	21.754399556917072	26.931646716383135	26.74677714660828	24.567176580091516
24-25	21.776840775668983	26.5902238057398	26.553804500036556	25.079130918554664
26-27	21.57338515346759	26.741287709478552	26.733614486907193	24.951712650146668
28-29	21.635972033202187	26.910077053566393	26.68801405619194	24.765936857039478
30-31	21.847130996021072	26.75167461488261	26.526322517485077	24.874871871611237
32-33	21.800307579756552	26.65499107445498	26.715357755153065	24.829343590635403
34-35	21.93524981963074	26.85211696160711	26.436609675671036	24.77602354309111
36-37	21.797669909497653	26.650385142677123	26.45412277852642	25.097822169298805
38-39	21.730075002360483	26.761472824749198	26.607648509391158	24.900803663499158
40-41	21.944086300460217	27.025969119732355	26.48343260469881	24.546511975108615
42-43	21.95492387095581	26.604631269398222	26.537736069347208	24.902708790298764
44-45	21.93916778762895	26.603637861118894	26.658479421918596	24.79871492933356
46-47	21.953679102261926	26.67793525222153	26.542244913546348	24.826140731970195
48-49	21.844651270018247	26.580812307666307	26.43344675505755	25.141089667257898
50-51	21.880281846946477	26.56021519618982	26.63344025011447	24.92606270674923
52-53	22.15543646623789	26.79480672933797	26.337027577466106	24.71272922695803
54-55	22.01150675086582	26.70370376291067	26.334552675388373	24.95023681083514
56-57	21.94660742182694	26.447916760037216	26.68423821933886	24.921237598796985
58-59	22.00985736041622	26.559103755289726	26.602201446329683	24.828837437964374
60-61	22.076966251343773	26.408293130431876	26.517153869942984	24.997586748281368
62-63	21.981828992367003	26.40485555167124	26.595026719853447	25.018288736108314
64-65	22.116764151231422	26.631927925485765	26.480620112107978	24.770687811174838
66-67	22.11851175850114	26.421440814095305	26.45821438670255	25.001833040701012
68-69	22.10222000011333	26.44963309630516	26.651865210048882	24.796281693532627
70-71	22.301583123835623	26.72917465848586	26.412354633578506	24.55688758410001
72-73	22.244914877185334	26.447390153736784	26.323185572605958	24.98450939647193
74-75	22.11684085662167	26.434210366306743	26.596742522904197	24.852206254167392
76-77	22.15657404223814	26.65780431699558	26.4731200141522	24.712501626614078
78-79	22.17696521102711	26.462726985409617	26.310225966559507	25.050081837003763
80-81	22.24690242659474	26.475403139291547	26.511292045143513	24.766402388970196
82-83	22.419389723020657	26.72967055471731	26.205009734863754	24.64592998739828
84-85	22.36559386878573	26.609514754934626	26.15331047304441	24.871580903235234
86-87	22.414323822229992	26.42949160456043	26.397181250225877	24.759003322983705
88-89	22.589179374537586	26.536564247224607	26.259603160796775	24.61465321744103
90-91	22.556084936613438	26.41549722629466	26.196327873161618	24.832089963930283
92-93	22.503183687609823	26.422020132127333	26.19579029723662	24.87900588302622
94-95	22.659240349823587	26.616189631820625	26.086170632030896	24.638399386324892
96-97	22.688336641466393	26.61236374718548	25.84354966578588	24.85574994556225
98-99	22.684505870237455	26.734970429361248	25.979557828493867	24.60096587190743
100-101	22.799913242343607	26.921718342832847	25.74706158109377	24.531306833729776
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	86.0
1	61.5
2	42.0
3	41.0
4	41.0
5	59.0
6	69.0
7	78.5
8	98.5
9	124.5
10	147.0
11	156.0
12	161.5
13	201.0
14	259.5
15	300.5
16	347.0
17	437.5
18	551.5
19	715.5
20	986.5
21	1378.5
22	2010.0
23	3035.5
24	4647.5
25	6948.5
26	10528.0
27	15857.0
28	22507.0
29	32099.5
30	46067.5
31	63829.5
32	90279.5
33	127483.5
34	173352.5
35	239293.0
36	331964.5
37	456234.5
38	621627.0
39	845631.0
40	1103429.0
41	1360930.0
42	1628981.5
43	1884559.5
44	2095004.5
45	2236743.5
46	2311126.5
47	2322314.0
48	2244837.0
49	2117516.5
50	1953306.5
51	1736308.5
52	1539269.5
53	1353137.0
54	1154555.0
55	970697.5
56	800144.5
57	647290.5
58	506991.5
59	384494.5
60	301009.0
61	250228.0
62	207687.5
63	173102.5
64	143709.5
65	118762.0
66	99862.5
67	79756.0
68	57843.5
69	41328.0
70	30370.0
71	22868.0
72	17978.0
73	14162.5
74	10048.5
75	5989.5
76	3379.5
77	1228.5
78	154.5
79	51.0
80	19.5
81	7.5
82	5.5
83	3.5
84	1.5
85	1.0
86	3.0
87	2.5
88	0.0
89	0.5
90	2.5
91	2.5
92	1.0
93	0.5
94	0.5
95	1.0
96	2.0
97	1.5
98	0.0
99	1.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.059869976558419226
2	0.0
3	4.56739438771138E-5
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	5.095499363790508E-4
12-13	0.0
14-15	0.0
16-17	0.0012645973210975883
18-19	0.0020324905025315637
20-21	0.0
22-23	0.0
24-25	1.2274872416974332E-4
26-27	0.0
28-29	2.5406131281644547E-4
30-31	0.0017727199467304792
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.002901722746942886
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0022423051822170557
48-49	0.0
50-51	0.0015814603067450651
52-53	4.4960288504033893E-4
54-55	0.0
56-57	6.85109158156707E-4
58-59	9.991175223118644E-5
60-61	0.0010804742348429733
62-63	0.0032999424451214715
64-65	0.001905459846123341
66-67	9.20615431273075E-4
68-69	3.6396424027075055E-4
70-71	8.563864476958837E-4
72-73	0.0
74-75	0.0
76-77	9.263246742577141E-4
78-79	1.4273107461598062E-6
80-81	0.001684226680468571
82-83	0.0010776196133506538
84-85	1.855503970007748E-5
86-87	9.93408279327225E-4
88-89	0.0
90-91	0.0019896711801467696
92-93	0.0011090204497661695
94-95	0.0
96-97	2.2266047640092979E-4
98-99	0.0011104477605123292
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3.5030914E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.34331660149843
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.03928242544612	17.300557069126985
2	15.564026819987948	8.822722795062624
3	6.844909017165156	5.820222701458904
4	3.9615480776497063	4.491336455875325
5	2.453717791823155	3.477325011218666
6	1.7606966716980632	2.994238992308565
7	1.3029412899445194	2.5850774245843597
8	0.9513779182308163	2.157216443527241
9	0.7654500691697349	1.9525854287805
>10	4.7330359727229725	25.036301723680225
>50	0.3843397636912635	7.492295636425316
>100	0.21573834765723315	11.392984321186491
>500	0.015656791246697766	3.0195838817847305
>1k	0.007215298454136623	3.352158486856869
>5k	6.374511268692946E-5	0.10539362812322375
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.56739438771138E-5	0.0	0.0	0.0	0.0
2	7.992940178494914E-5	8.563864476958838E-6	5.709242984639225E-6	0.0	8.563864476958838E-6
3	1.4558569610830023E-4	8.563864476958838E-6	5.709242984639225E-6	0.0	8.563864476958838E-6
4	2.6833442027804355E-4	8.563864476958838E-6	5.709242984639225E-6	0.0	1.141848596927845E-5
5	3.0544449967819854E-4	8.563864476958838E-6	8.563864476958838E-6	0.0	1.141848596927845E-5
6	3.7681003698618884E-4	8.563864476958838E-6	1.141848596927845E-5	0.0	1.141848596927845E-5
7	4.224839808633026E-4	8.563864476958838E-6	1.141848596927845E-5	0.0	1.141848596927845E-5
8	4.824310322020145E-4	8.563864476958838E-6	1.4273107461598062E-5	0.0	1.7127728953917675E-5
9	5.166864901098499E-4	1.4273107461598062E-5	1.4273107461598062E-5	0.0	2.8546214923196123E-5
10-11	6.023251348794383E-4	1.9982350446237286E-5	1.4273107461598062E-5	0.0	2.8546214923196123E-5
12-13	6.736906721874286E-4	3.140083641551574E-5	1.7127728953917675E-5	2.8546214923196124E-6	2.8546214923196123E-5
14-15	8.078578823264503E-4	3.140083641551574E-5	1.7127728953917675E-5	5.709242984639225E-6	2.8546214923196123E-5
16-17	9.134788775422759E-4	3.140083641551574E-5	1.7127728953917675E-5	5.709242984639225E-6	2.8546214923196123E-5
18-19	0.0010133906297734624	3.140083641551574E-5	1.7127728953917675E-5	5.709242984639225E-6	2.8546214923196123E-5
20-21	0.0011247208679739272	3.2828147161675544E-5	1.7127728953917675E-5	5.709242984639225E-6	3.140083641551574E-5
22-23	0.0012331964846820725	3.568276865399515E-5	1.7127728953917675E-5	5.709242984639225E-6	3.711007940015496E-5
24-25	0.0013616544518364549	3.711007940015496E-5	1.7127728953917675E-5	5.709242984639225E-6	3.711007940015496E-5
26-27	0.0015029582157062757	4.56739438771138E-5	1.7127728953917675E-5	5.709242984639225E-6	3.711007940015496E-5
28-29	0.001738464488822644	4.56739438771138E-5	3.2828147161675544E-5	5.709242984639225E-6	4.2819322384794185E-5
30-31	0.0021167018365549923	4.56739438771138E-5	3.425545790783535E-5	5.709242984639225E-6	4.56739438771138E-5
32-33	0.0026419521911418015	5.423780835407263E-5	3.425545790783535E-5	5.709242984639225E-6	4.56739438771138E-5
34-35	0.0033998541973526584	6.137436208487166E-5	3.425545790783535E-5	5.709242984639225E-6	4.56739438771138E-5
36-37	0.004538848172788184	6.565629432335109E-5	3.425545790783535E-5	5.709242984639225E-6	4.56739438771138E-5
38-39	0.006225929474749075	7.707478029262953E-5	3.425545790783535E-5	5.709242984639225E-6	4.995587611559322E-5
40-41	0.008733714455751853	8.135671253110894E-5	3.425545790783535E-5	5.709242984639225E-6	5.423780835407263E-5
42-43	0.012056493872811883	9.13478877542276E-5	3.425545790783535E-5	5.709242984639225E-6	5.566511910023244E-5
44-45	0.016115765634890374	9.562981999270702E-5	3.425545790783535E-5	5.709242984639225E-6	5.7092429846392247E-5
46-47	0.020664604982901673	9.991175223118644E-5	3.425545790783535E-5	5.709242984639225E-6	5.7092429846392247E-5
48-49	0.026620772726626545	9.991175223118644E-5	3.425545790783535E-5	5.709242984639225E-6	5.7092429846392247E-5
50-51	0.0350704523438926	1.0704830596198545E-4	3.711007940015496E-5	5.709242984639225E-6	5.7092429846392247E-5
52-53	0.04609357323648478	1.1989410267742372E-4	3.8537390146314764E-5	5.709242984639225E-6	5.994705133871186E-5
54-55	0.058378436828682226	1.2560334566206295E-4	4.424663313095399E-5	5.709242984639225E-6	6.280167283103147E-5
56-57	0.07207919268107021	1.5129493909293946E-4	5.7092429846392247E-5	5.709242984639225E-6	6.280167283103147E-5
58-59	0.08781814827897438	1.5557687133141887E-4	6.280167283103147E-5	8.563864476958838E-6	6.422898357719129E-5
60-61	0.10837570495591409	1.5985880356989828E-4	6.280167283103147E-5	8.563864476958838E-6	6.708360506951089E-5
62-63	0.13511208985297957	1.5985880356989828E-4	6.280167283103147E-5	8.563864476958838E-6	6.85109158156707E-5
64-65	0.16822712647463323	1.5985880356989828E-4	6.280167283103147E-5	8.563864476958838E-6	7.13655373079903E-5
66-67	0.20674310695975562	1.612861143160581E-4	6.422898357719129E-5	8.563864476958838E-6	7.422015880030992E-5
68-69	0.25063005778267733	1.627134250622179E-4	6.565629432335109E-5	1.141848596927845E-5	7.850209103878934E-5
70-71	0.30406286287591583	1.627134250622179E-4	6.565629432335109E-5	1.141848596927845E-5	8.278402327726876E-5
72-73	0.37231258082503926	1.6414073580837773E-4	6.85109158156707E-5	1.141848596927845E-5	8.563864476958837E-5
74-75	0.4582709432017674	1.6984997879301693E-4	6.85109158156707E-5	1.141848596927845E-5	8.563864476958837E-5
76-77	0.5637820354901388	1.7413191103149634E-4	6.85109158156707E-5	1.141848596927845E-5	8.563864476958837E-5
78-79	0.6903516705273519	1.7841384326997575E-4	6.85109158156707E-5	1.141848596927845E-5	9.13478877542276E-5
80-81	0.8386578209178328	1.7984115401613557E-4	6.85109158156707E-5	1.141848596927845E-5	9.562981999270702E-5
82-83	1.0191712382954097	1.7984115401613557E-4	6.85109158156707E-5	1.141848596927845E-5	9.848444148502662E-5
84-85	1.2450274634569913	1.8840501849309442E-4	6.85109158156707E-5	1.141848596927845E-5	9.991175223118644E-5
86-87	1.5247304138281976	1.9982350446237288E-4	6.85109158156707E-5	1.141848596927845E-5	1.0276637372350604E-4
88-89	1.8550971864450925	2.0553274744701208E-4	7.422015880030992E-5	1.141848596927845E-5	1.0562099521582565E-4
90	2.1344318906437896	2.112419904316513E-4	7.707478029262953E-5	1.141848596927845E-5	1.0562099521582565E-4
>>END_MODULE
