##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654135_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29021727
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.389144863777403	32.0	32.0	32.0	32.0	32.0
2	31.416335044430678	32.0	32.0	32.0	32.0	32.0
3	35.30235499079707	37.0	37.0	37.0	32.0	37.0
4	35.92989535047311	37.0	37.0	37.0	32.0	37.0
5	36.12448618236951	37.0	37.0	37.0	37.0	37.0
6	39.35370703473298	41.0	41.0	41.0	37.0	41.0
7	39.532088631389854	41.0	41.0	41.0	37.0	41.0
8	39.63096062477605	41.0	41.0	41.0	37.0	41.0
9	39.64289440804126	41.0	41.0	41.0	37.0	41.0
10-11	39.73405187086213	41.0	41.0	41.0	37.0	41.0
12-13	39.696413983220225	41.0	41.0	41.0	37.0	41.0
14-15	39.68439958793631	41.0	41.0	41.0	37.0	41.0
16-17	39.61300840229115	41.0	41.0	41.0	37.0	41.0
18-19	39.60285898906017	41.0	41.0	41.0	37.0	41.0
20-21	39.56949381406558	41.0	41.0	41.0	37.0	41.0
22-23	39.4827178100049	41.0	41.0	41.0	37.0	41.0
24-25	39.48924440299504	41.0	41.0	41.0	37.0	41.0
26-27	39.37907695844565	41.0	41.0	41.0	37.0	41.0
28-29	39.41212464716521	41.0	41.0	41.0	37.0	41.0
30-31	39.477185833909886	41.0	41.0	41.0	37.0	41.0
32-33	39.45551208927022	41.0	41.0	41.0	37.0	41.0
34-35	39.30490843980443	41.0	41.0	41.0	37.0	41.0
36-37	39.18197039066628	41.0	41.0	41.0	37.0	41.0
38-39	39.22505924612963	41.0	41.0	41.0	37.0	41.0
40-41	39.19172957901506	41.0	41.0	41.0	37.0	41.0
42-43	39.0590881445477	41.0	41.0	41.0	37.0	41.0
44-45	39.07379659039587	41.0	41.0	41.0	37.0	41.0
46-47	39.04677361205968	41.0	41.0	41.0	37.0	41.0
48-49	39.07645327240519	41.0	41.0	41.0	37.0	41.0
50-51	38.98225414014817	41.0	41.0	41.0	37.0	41.0
52-53	39.055675115405776	41.0	41.0	41.0	37.0	41.0
54-55	38.92720057631305	41.0	41.0	41.0	34.5	41.0
56-57	38.91128034868497	41.0	41.0	41.0	34.5	41.0
58-59	38.89817614920022	41.0	41.0	41.0	32.0	41.0
60-61	38.909519702256176	41.0	41.0	41.0	34.5	41.0
62-63	38.920806659782855	41.0	41.0	41.0	37.0	41.0
64-65	38.91301422206887	41.0	41.0	41.0	37.0	41.0
66-67	38.856960011373545	41.0	41.0	41.0	32.0	41.0
68-69	38.807871823065526	41.0	41.0	41.0	32.0	41.0
70-71	38.76229838079588	41.0	41.0	41.0	32.0	41.0
72-73	38.7189706525735	41.0	41.0	41.0	32.0	41.0
74-75	38.69534504269853	41.0	41.0	41.0	32.0	41.0
76-77	37.88540320153932	41.0	39.0	41.0	32.0	41.0
78-79	38.43837734053525	41.0	39.0	41.0	32.0	41.0
80-81	38.84049650112138	41.0	41.0	41.0	32.0	41.0
82-83	38.876194169974795	41.0	41.0	41.0	32.0	41.0
84-85	38.75622043443521	41.0	41.0	41.0	32.0	41.0
86-87	38.79705795936954	41.0	41.0	41.0	32.0	41.0
88-89	38.677953538050986	41.0	41.0	41.0	32.0	41.0
90-91	38.58360124468127	41.0	41.0	41.0	32.0	41.0
92-93	38.56488630052925	41.0	41.0	41.0	32.0	41.0
94-95	38.38917823532693	41.0	39.0	41.0	32.0	41.0
96-97	38.354161539042806	41.0	37.0	41.0	32.0	41.0
98-99	38.278336985252466	41.0	37.0	41.0	32.0	41.0
100-101	37.17429963764734	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	41.0
13	149.0
14	551.0
15	2018.0
16	7914.0
17	20322.0
18	34153.0
19	45207.0
20	53779.0
21	63523.0
22	74867.0
23	87185.0
24	101519.0
25	116242.0
26	134667.0
27	154162.0
28	177363.0
29	206192.0
30	240120.0
31	282673.0
32	333230.0
33	399796.0
34	493406.0
35	627846.0
36	814849.0
37	1134155.0
38	1866405.0
39	4604210.0
40	1.6945183E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	36.29547822698535	21.98573678582015	10.255418882156185	31.46336610503831
2	29.489296468848337	25.160196057127536	29.341191249083515	16.00931622494061
3	20.871388768096217	27.456237255033034	29.523721575148702	22.148652401722046
4	24.839702616843322	31.62329932336111	23.165058237144336	20.37193982265123
5	27.329054741500563	33.26765225842579	21.316429459735165	18.08686354033848
6	22.63294739916801	36.90969978537467	21.643014876915625	18.8143379385417
7	21.926643810662195	21.681097917980477	36.29247411039407	20.09978416096326
8	22.95815087115085	24.75758060392583	26.80330736106348	25.480961163859835
9	23.397930426285544	23.3911146155757	29.270919482154135	23.940035475984622
10-11	25.23127847933921	30.047412577154354	23.22278129009812	21.498527653408324
12-13	25.532862723714818	24.99218566212088	26.28432274113086	23.190628873033436
14-15	24.72030245079635	26.637483422461923	26.3391592420604	22.30305488468133
16-17	25.57812273660681	26.33373340189814	25.70852519556751	22.37961866592754
18-19	25.070194915047324	26.811337927237073	25.911076177177854	22.207390980537753
20-21	25.224341938068306	26.78842345116924	25.776825365374595	22.21040924538786
22-23	25.271542970707216	26.579602706680316	25.85271236621427	22.296141956398195
24-25	24.83190012928042	26.6402408272155	26.207881126109495	22.319977917394585
26-27	24.982301980065806	26.82360421524225	26.010963312968705	22.18313049172324
28-29	25.202112159507216	26.5263538542576	25.920484298673824	22.351049687561357
30-31	24.798988121248804	26.531464164588954	26.28605517174193	22.383492542420313
32-33	24.8985636401942	26.795846033410008	26.090188457226603	22.215401869169185
34-35	25.193830063446004	26.69646790970145	25.760096409867504	22.34960561698504
36-37	24.85498175739661	26.584166526772794	26.271905825632334	22.28894589019826
38-39	24.98591167026475	26.801248050438097	26.065725758415287	22.147114520881864
40-41	25.206296410263356	26.524924190894712	25.960060141755225	22.308719257086707
42-43	24.6835600948758	26.667828698096102	26.37540479840409	22.27320640862401
44-45	24.863958721013315	26.672169782631343	26.19650558970771	22.26736590664763
46-47	25.21946760588462	26.51518466835945	25.98124750348513	22.2841002222708
48-49	24.7919464888904	26.517528044279853	26.344043937177062	22.34648152965269
50-51	24.999176390939585	26.601214902623564	26.06065856607526	22.338950140361593
52-53	25.23176135737002	26.55164700208492	25.986493695743384	22.230097944801678
54-55	24.72480099673719	26.594095028263258	26.388591211885654	22.2925127631139
56-57	24.794545133491386	26.73017299713985	26.21594654251774	22.259335326851023
58-59	25.208507954308473	26.4342283162272	26.037625918349537	22.31963781111479
60-61	24.882888730463442	26.53707986711628	26.30929318228622	22.270738220134056
62-63	24.918888498766048	26.60250471729966	26.296169931237852	22.182436852696448
64-65	25.118314327961127	26.492621473629484	26.17441101793042	22.21465318047897
66-67	24.819685876373438	26.539765179163876	26.456232878997017	22.18431606546567
68-69	24.866983471110693	26.763529910029632	26.234039855961804	22.13544676289787
70-71	25.273816256654364	26.562219024232025	25.989704500824217	22.174260218289394
72-73	24.827932840710858	26.563806884643643	26.489289288730493	22.118970985915006
74-75	24.794535652133217	26.794599372026497	26.331634199359282	22.079230776481005
76-77	25.207363441508196	26.51838188932646	26.119276193460678	22.154978475704663
78-79	24.81952907112386	26.639582300209145	26.4166928286162	22.124195800050796
80-81	25.05911821943436	26.69399194668724	26.28222622837863	21.964663605499762
82-83	25.281014809283814	26.421249320782348	26.179974462432725	22.117761407501114
84-85	24.994562636242105	26.573429706405793	26.44900876281442	21.98299889453768
86-87	25.035798944540606	26.80187216841866	26.172454413954643	21.989874473086086
88-89	25.30453846023294	26.586875035698952	26.060421540139235	22.048164963928873
90-91	24.915238801172226	26.666796803889536	26.406950788729777	22.01101360620846
92-93	25.105610008256367	26.80292057383363	26.14221801444173	21.949251403468267
94-95	25.459034675256863	26.706019195242863	25.971493760192637	21.86345236930763
96-97	25.16451778587026	26.594524431690854	26.365998001860376	21.874959780578514
98-99	25.28323326932338	26.87797015223686	26.025900339175212	21.81289623926455
100-101	25.65556881542552	26.59721026510706	25.939608704206645	21.80761221526078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	33.0
2	19.5
3	31.5
4	29.0
5	35.5
6	52.5
7	61.0
8	78.5
9	106.0
10	131.5
11	155.5
12	201.5
13	256.0
14	300.0
15	363.0
16	448.5
17	555.0
18	709.0
19	889.0
20	1165.0
21	1546.5
22	1975.5
23	2591.5
24	3501.5
25	4784.0
26	6416.5
27	8803.0
28	12413.5
29	17292.0
30	24152.0
31	34119.0
32	49455.5
33	72508.0
34	107955.0
35	164634.0
36	245538.5
37	357906.5
38	520709.0
39	733876.5
40	961766.5
41	1190783.0
42	1429189.0
43	1635392.0
44	1788926.5
45	1890438.0
46	1926546.0
47	1912628.0
48	1838098.5
49	1718263.5
50	1558916.5
51	1390336.5
52	1235694.0
53	1083959.5
54	941897.0
55	796473.5
56	651746.0
57	516905.5
58	407030.5
59	323993.0
60	268251.0
61	229555.0
62	194414.0
63	159592.5
64	129399.0
65	106734.0
66	90770.5
67	75176.5
68	55802.5
69	39923.5
70	30401.0
71	23140.0
72	16107.5
73	10413.5
74	6086.0
75	3278.0
76	1887.0
77	1080.5
78	682.5
79	510.5
80	411.5
81	322.0
82	261.5
83	212.5
84	171.5
85	160.5
86	139.0
87	106.5
88	92.5
89	83.0
90	72.0
91	61.5
92	58.0
93	49.5
94	43.5
95	44.5
96	44.5
97	47.5
98	71.5
99	142.5
100	607.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007515059320901199
2	0.006264272281246392
3	0.0016091392493630722
4	1.4816485593707087E-4
5	0.01459596115696354
6	0.0015712366117977747
7	0.005134084542935712
8	0.009303374675118404
9	0.0033354321057461533
10-11	0.006531313591365531
12-13	0.004665470114855673
14-15	0.009620378552937254
16-17	1.2059930134412744E-4
18-19	0.01301611030935547
20-21	0.006381425888266401
22-23	0.008831314552714247
24-25	0.011756709033890369
26-27	0.010144124090203177
28-29	0.0067156582377058405
30-31	0.011131315514062963
32-33	0.005378728839948084
34-35	0.005034159407536292
36-37	0.009322325993901053
38-39	0.010433562413429083
40-41	0.009029441976351029
42-43	0.012332139986018061
44-45	0.008304123321124204
46-47	0.004379477485953885
48-49	0.010097607216827587
50-51	0.010698880876386164
52-53	0.007468542447525608
54-55	0.010490416369777029
56-57	0.01266292664113338
58-59	0.005964496875048133
60-61	0.0024653942889063768
62-63	0.0130988069731343
64-65	0.005056556420643058
66-67	0.006253935198274037
68-69	0.015720980353788043
70-71	0.006079927634906083
72-73	0.0059800024995066625
74-75	0.014132515270369678
76-77	0.006359028875159634
78-79	0.012945473575711052
80-81	1.89513187826486E-5
82-83	0.007327268980236772
84-85	0.016911467742770785
86-87	0.002136330480953115
88-89	0.01040427401167408
90-91	0.0037144584813991258
92-93	0.012147795339677752
94-95	0.010070041662234642
96-97	0.004246818254475345
98-99	0.009654835496178432
100-101	0.010652364003010572
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.9021727E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.89434317730529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.72407357143983	20.961615447147924
2	14.719629334087234	9.683850775163902
3	6.844010410905659	6.753876814961428
4	3.7238578726131384	4.899754352209864
5	2.3770968174219096	3.909651923897825
6	1.640239993935661	3.237277035219647
7	1.155937585778422	2.6616665346698833
8	0.8612667178102702	2.2664642386273903
9	0.6674602850517802	1.976010090634375
>10	3.8555855637328698	23.063774368781594
>50	0.26389913894872835	5.958819841741064
>100	0.14958581021454792	9.396737058613224
>500	0.01299443744445393	2.8782353828129295
>1k	0.00431826066440769	2.2731215457550027
>5k	4.4199950816554324E-5	0.07914458976380886
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1011248917061345E-5	0.0	1.7228471620589636E-5	3.4456943241179274E-6	0.0
2	3.79026375652972E-5	3.445694324117927E-5	1.7228471620589636E-5	6.891388648235855E-6	0.0
3	9.99251353994199E-5	3.445694324117927E-5	1.7228471620589636E-5	6.891388648235855E-6	0.0
4	1.791761048541322E-4	3.445694324117927E-5	3.79026375652972E-5	6.891388648235855E-6	3.4456943241179274E-6
5	2.0329596512295772E-4	3.445694324117927E-5	3.79026375652972E-5	6.891388648235855E-6	3.4456943241179274E-6
6	2.549813799847266E-4	3.445694324117927E-5	4.823972053765098E-5	6.891388648235855E-6	3.4456943241179274E-6
7	3.066667948464955E-4	3.445694324117927E-5	5.168541486176891E-5	6.891388648235855E-6	3.4456943241179274E-6
8	3.411237380876748E-4	3.445694324117927E-5	7.235958080647647E-5	1.0337082972353781E-5	6.891388648235855E-6
9	3.7558068132885405E-4	5.168541486176891E-5	7.925096945471232E-5	1.0337082972353781E-5	6.891388648235855E-6
10-11	4.3932602632503577E-4	5.513110918588684E-5	8.614235810294818E-5	1.378277729647171E-5	1.5505624458530673E-5
12-13	5.030713713212174E-4	5.513110918588684E-5	1.0509367688559679E-4	1.7228471620589636E-5	1.7228471620589636E-5
14-15	5.961051180724015E-4	5.513110918588684E-5	1.137079126958916E-4	1.7228471620589636E-5	1.7228471620589636E-5
16-17	6.805246290132906E-4	5.513110918588684E-5	1.6711617471971946E-4	1.89513187826486E-5	1.89513187826486E-5
18-19	8.02846777519477E-4	6.546819215824062E-5	2.0329596512295772E-4	2.756555459294342E-5	2.0674165944707563E-5
20-21	9.803000352115502E-4	7.925096945471232E-5	2.2569297822972424E-4	2.756555459294342E-5	2.2397013106766526E-5
22-23	0.001142247668445093	7.925096945471232E-5	2.567042271467856E-4	3.445694324117927E-5	2.411986026882549E-5
24-25	0.0013627721051886403	8.441951094088921E-5	2.6359561579502146E-4	3.79026375652972E-5	2.411986026882549E-5
26-27	0.001703895843276315	9.303374675118403E-5	2.6704131011913933E-4	4.1348331889415125E-5	2.411986026882549E-5
28-29	0.0021897387429769427	9.647944107530197E-5	2.9977540619825966E-4	4.6516873375592015E-5	2.411986026882549E-5
30-31	0.00284269781739729	1.137079126958916E-4	3.0838964200855445E-4	4.996256769970995E-5	2.411986026882549E-5
32-33	0.0036162561931617643	1.1715360702000953E-4	3.1355818349473137E-4	5.168541486176891E-5	2.5842707430884456E-5
34-35	0.00457243636810449	1.1887645418206849E-4	3.3767804376355685E-4	5.168541486176891E-5	3.445694324117927E-5
36-37	0.005859403198162536	1.2404499566824538E-4	3.462922795738517E-4	5.168541486176891E-5	4.1348331889415125E-5
38-39	0.007570190430087087	1.3093638431648123E-4	3.5318366822208755E-4	5.513110918588684E-5	4.1348331889415125E-5
40-41	0.009830565906708446	1.464420087750119E-4	3.721349870047361E-4	5.513110918588684E-5	4.1348331889415125E-5
42-43	0.012828319968691042	1.516105502611888E-4	4.048690830838565E-4	5.8576803510004764E-5	4.479402621353305E-5
44-45	0.016055212703227482	1.602247860714836E-4	4.1520616605621023E-4	6.029965067206373E-5	4.6516873375592015E-5
46-47	0.019576712302476006	1.7228471620589635E-4	4.3415748483885885E-4	6.202249783412269E-5	4.823972053765098E-5
48-49	0.02418705130814579	1.843446463403091E-4	4.4449456781121263E-4	6.202249783412269E-5	4.823972053765098E-5
50-51	0.030172222349138633	2.0501881228501666E-4	4.4794026213533056E-4	6.719103932029958E-5	4.823972053765098E-5
52-53	0.037385783416679516	2.1707874241942943E-4	4.668915809179792E-4	7.235958080647647E-5	4.996256769970995E-5
54-55	0.045257472100126914	2.1707874241942943E-4	4.875657468626867E-4	7.235958080647647E-5	5.513110918588684E-5
56-57	0.05410773797162381	2.188015895814884E-4	5.134084542935712E-4	7.235958080647647E-5	5.513110918588684E-5
58-59	0.0647635476689585	2.2052443674354735E-4	5.340826202382787E-4	7.235958080647647E-5	5.513110918588684E-5
60-61	0.07769179277304897	2.2224728390560631E-4	5.68539563479458E-4	7.235958080647647E-5	5.513110918588684E-5
62-63	0.09387794186059292	2.3086151971590113E-4	6.305620613135806E-4	7.235958080647647E-5	5.513110918588684E-5
64-65	0.11270349280041123	2.3775290836413698E-4	6.684646988788779E-4	7.235958080647647E-5	5.513110918588684E-5
66-67	0.13489031855340655	2.4981283849854975E-4	6.736332403650548E-4	7.235958080647647E-5	6.029965067206373E-5
68-69	0.1606916776524016	2.704870044432573E-4	6.788017818512317E-4	7.58052751305944E-5	6.719103932029958E-5
70-71	0.19151341338163647	2.84269781739729E-4	6.874160176615265E-4	7.58052751305944E-5	7.235958080647647E-5
72-73	0.22981230579420722	2.8599262890178796E-4	7.063673364441751E-4	7.58052751305944E-5	7.58052751305944E-5
74-75	0.277841838978087	2.8599262890178796E-4	7.063673364441751E-4	7.58052751305944E-5	7.58052751305944E-5
76-77	0.33504036475844456	2.877154760638469E-4	7.09813030768293E-4	7.58052751305944E-5	7.58052751305944E-5
78-79	0.4020315538079453	2.928840175500238E-4	7.167044194165289E-4	7.58052751305944E-5	7.752812229265336E-5
80-81	0.4815788529745318	2.928840175500238E-4	7.235958080647647E-4	7.58052751305944E-5	8.097381661677129E-5
82-83	0.5780445112725373	3.032211005223776E-4	7.270415023888827E-4	7.58052751305944E-5	8.786520526500714E-5
84-85	0.6971948981533732	3.0838964200855445E-4	7.304871967130006E-4	7.58052751305944E-5	9.647944107530197E-5
86-87	0.8427944346661382	3.170038778188493E-4	7.425471268474133E-4	7.752812229265336E-5	1.0164798256147886E-4
88-89	1.014862072129615	3.170038778188493E-4	7.511613626577081E-4	7.925096945471232E-5	1.0681652404765575E-4
90	1.159958537271059	3.411237380876748E-4	7.546070569818261E-4	8.269666377883025E-5	1.0681652404765575E-4
>>END_MODULE
