##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654113_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	24273431
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.138251613461648	32.0	32.0	32.0	32.0	32.0
2	31.302979418113573	32.0	32.0	32.0	32.0	32.0
3	35.14453856976379	37.0	37.0	37.0	32.0	37.0
4	35.81049489872281	37.0	37.0	37.0	32.0	37.0
5	35.92632310611549	37.0	37.0	37.0	37.0	37.0
6	39.3489589502201	41.0	41.0	41.0	37.0	41.0
7	39.423029566772	41.0	41.0	41.0	37.0	41.0
8	39.45875801406072	41.0	41.0	41.0	37.0	41.0
9	39.54757207582232	41.0	41.0	41.0	37.0	41.0
10-11	39.579596720381225	41.0	41.0	41.0	37.0	41.0
12-13	39.569032021060394	41.0	41.0	41.0	37.0	41.0
14-15	39.46462473722812	41.0	41.0	41.0	37.0	41.0
16-17	39.420338187872986	41.0	41.0	41.0	37.0	41.0
18-19	39.25072176652736	41.0	41.0	41.0	37.0	41.0
20-21	39.14912994788417	41.0	41.0	41.0	37.0	41.0
22-23	38.99096448293609	41.0	41.0	41.0	37.0	41.0
24-25	39.02272284869824	41.0	41.0	41.0	37.0	41.0
26-27	39.025741540205004	41.0	41.0	41.0	37.0	41.0
28-29	39.16252737406591	41.0	41.0	41.0	37.0	41.0
30-31	39.17128357338524	41.0	41.0	41.0	37.0	41.0
32-33	39.234904307512195	41.0	41.0	41.0	37.0	41.0
34-35	39.23954847998208	41.0	41.0	41.0	37.0	41.0
36-37	39.2467469061131	41.0	41.0	41.0	37.0	41.0
38-39	39.23050540321226	41.0	41.0	41.0	37.0	41.0
40-41	39.16625735768462	41.0	41.0	41.0	37.0	41.0
42-43	39.15725609618187	41.0	41.0	41.0	37.0	41.0
44-45	39.147583854132534	41.0	41.0	41.0	37.0	41.0
46-47	39.09574738321913	41.0	41.0	41.0	37.0	41.0
48-49	39.05431436536516	41.0	41.0	41.0	37.0	41.0
50-51	39.087813523353994	41.0	41.0	41.0	37.0	41.0
52-53	39.08110664289691	41.0	41.0	41.0	37.0	41.0
54-55	39.06798637160111	41.0	41.0	41.0	37.0	41.0
56-57	39.02545313845414	41.0	41.0	41.0	37.0	41.0
58-59	38.928826728285756	41.0	41.0	41.0	34.5	41.0
60-61	38.95769753357076	41.0	41.0	41.0	37.0	41.0
62-63	38.94192409387861	41.0	41.0	41.0	37.0	41.0
64-65	38.910364711111505	41.0	41.0	41.0	34.5	41.0
66-67	38.85953326911222	41.0	41.0	41.0	32.0	41.0
68-69	38.8124797849962	41.0	41.0	41.0	32.0	41.0
70-71	38.77177134538583	41.0	41.0	41.0	32.0	41.0
72-73	38.742942664347694	41.0	41.0	41.0	32.0	41.0
74-75	38.71425458971993	41.0	41.0	41.0	32.0	41.0
76-77	37.96825864872584	41.0	39.0	41.0	32.0	41.0
78-79	38.40616882714273	41.0	39.0	41.0	32.0	41.0
80-81	38.71562965284966	41.0	41.0	41.0	32.0	41.0
82-83	38.86355527984486	41.0	41.0	41.0	32.0	41.0
84-85	38.808157363497564	41.0	41.0	41.0	32.0	41.0
86-87	38.74362927927247	41.0	41.0	41.0	32.0	41.0
88-89	38.67900085900506	41.0	41.0	41.0	32.0	41.0
90-91	38.61613939125458	41.0	41.0	41.0	32.0	41.0
92-93	38.5412388137466	41.0	41.0	41.0	32.0	41.0
94-95	38.52471939380963	41.0	41.0	41.0	32.0	41.0
96-97	38.41756591806078	41.0	37.0	41.0	32.0	41.0
98-99	38.20487509573739	41.0	37.0	41.0	32.0	41.0
100-101	37.24242143601373	41.0	37.0	41.0	29.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	34.0
13	177.0
14	601.0
15	1560.0
16	5217.0
17	13253.0
18	21981.0
19	29991.0
20	37656.0
21	46275.0
22	57202.0
23	69400.0
24	83676.0
25	99280.0
26	117244.0
27	137736.0
28	161039.0
29	188723.0
30	226393.0
31	273695.0
32	311933.0
33	366598.0
34	446017.0
35	556326.0
36	723228.0
37	1004291.0
38	1627578.0
39	3916793.0
40	1.3749534E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	31.88249699955158	22.696360390124237	10.417260161958271	35.00388244836591
2	29.062381004152236	25.535640182057495	29.56617875734172	15.835800056448551
3	20.21201581419776	27.92866736553932	29.33362220948399	22.525694610778938
4	24.343855633758572	30.759965494783163	23.845500044884467	21.050678826573797
5	27.599726494419347	32.57909862639914	21.85811988644805	17.96305499273346
6	22.465814577263508	36.96464253446495	21.909247192949362	18.660295695322183
7	22.186274367228926	22.78135711428681	35.074625420691454	19.95774309779281
8	22.554248717455724	24.898103609662765	27.683717229756272	24.86393044312524
9	23.202793210403588	23.323250017683943	30.0200824514672	23.453874320445266
10-11	24.901359540696873	29.966869263765332	23.695835291840158	21.435935903697636
12-13	25.47191041624881	25.188142187574957	26.228654530947043	23.111292865229185
14-15	24.78605517283486	26.732918391306114	26.11244780352642	22.36857863233261
16-17	25.649889972122097	26.287419072414803	25.597548222643308	22.4651427328198
18-19	25.158882086914353	26.906913076607175	25.647842740613598	22.286362095864877
20-21	25.47486937801424	26.831004582192563	25.48157021255138	22.212555827241815
22-23	25.269314984755088	26.715998698654737	25.64220552562537	22.372480790964804
24-25	24.83409203488715	26.775446401875158	25.982228732136914	22.408232831100776
26-27	25.131647025918998	26.86870883642284	25.83297556904914	22.166668568609026
28-29	25.193132499963898	26.48532210303516	25.88352359998391	22.438021797017033
30-31	24.916920479844816	26.495634259532576	26.08666652851836	22.50077873210425
32-33	25.091156451262243	26.76490932187214	25.79637241345385	22.34756181341177
34-35	25.181431912118235	26.68561770274668	25.66642721418328	22.46652317095181
36-37	24.842847721032925	26.545017471984078	26.174828766481344	22.43730604050165
38-39	25.179688165608997	26.731570092783368	25.885240913275904	22.20350082833173
40-41	25.317152321812276	26.471399943419616	25.764095730842502	22.44735200392561
42-43	24.811361557894635	26.577309755827415	26.200499435169718	22.410829251108233
44-45	24.93631662231736	26.622789074868958	26.13799122571757	22.302903077096108
46-47	25.29924108975524	26.416279287110793	25.824937749321226	22.45954187381274
48-49	24.863808652121804	26.476871453160953	26.20462368037354	22.454696214343695
50-51	25.09694859371137	26.570703993184974	25.987086456793023	22.345260956310625
52-53	25.303931818797455	26.383299984787417	25.813583156119297	22.49918504029583
54-55	24.72530431051548	26.490415503609622	26.32250477530572	22.461775410569178
56-57	24.874253314682953	26.673025824007045	26.08459502992015	22.368125831389854
58-59	25.19906435971083	26.45037283769237	25.824837040960546	22.525725761636252
60-61	24.93072310133756	26.546170959362204	26.102177140360936	22.4209287989393
62-63	24.970798413721056	26.5577530170372	26.173165901017665	22.298282668224083
64-65	25.152023214188386	26.450028840175086	25.972657099855397	22.425290845781134
66-67	24.89097596620159	26.569336146186984	26.258678468819845	22.28100941879158
68-69	25.045917165999004	26.78592568028214	25.98713744647186	22.181019707247003
70-71	25.33887065239437	26.523843171173343	25.78293228082826	22.35435389560403
72-73	24.850370349375	26.611804486971785	26.202760540938776	22.33506462271444
74-75	24.894950747683385	26.769924793320882	26.07993218578248	22.255192273213254
76-77	25.416035647947655	26.427724161857558	25.765367967847023	22.390872222347763
78-79	24.87628757549767	26.65484125420918	26.090675026534154	22.378196143758995
80-81	25.117595730980447	26.694428172572632	25.98094873661026	22.207027359836665
82-83	25.378376110825712	26.366364729586394	25.884403286131086	22.370855873456808
84-85	25.028151395377098	26.605945826014004	26.195026605012682	22.170876173596216
86-87	25.146267950336316	26.85138330877081	25.838893562265675	22.1634551786272
88-89	25.32636526056508	26.536021001280137	25.817277310968684	22.320336427186096
90-91	24.914184430077153	26.73762122904676	26.170698679623722	22.17749566125237
92-93	25.180809510485087	26.8545782087802	25.871163732912528	22.093448547822188
94-95	25.52630899191795	26.642276075434086	25.70397032047097	22.127444612176994
96-97	25.206515690594756	26.634279456147986	26.08735569732985	22.071849155927406
98-99	25.376079506210587	26.938788550416525	25.711897388026152	21.973234555346732
100-101	25.628990742098356	26.66659141223631	25.665374473941664	22.039043371723665
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	22.0
2	21.5
3	24.0
4	34.5
5	48.0
6	61.5
7	80.5
8	92.0
9	103.0
10	119.5
11	147.0
12	192.0
13	235.0
14	287.0
15	356.5
16	433.5
17	536.5
18	684.5
19	831.5
20	1026.5
21	1296.0
22	1701.0
23	2191.0
24	2748.0
25	3656.5
26	5047.5
27	6855.0
28	9465.0
29	13318.5
30	18530.5
31	26065.0
32	38140.5
33	56896.5
34	86269.0
35	132418.5
36	200359.5
37	297847.5
38	434147.5
39	605300.0
40	796502.0
41	991138.5
42	1185257.5
43	1354534.0
44	1486880.0
45	1587604.5
46	1608663.5
47	1572283.5
48	1513095.0
49	1421668.5
50	1296412.0
51	1171052.0
52	1038370.0
53	889369.5
54	762586.0
55	654160.5
56	547627.0
57	443005.0
58	355453.0
59	281243.5
60	235067.5
61	207533.0
62	181886.0
63	152762.0
64	122650.0
65	101731.5
66	87879.0
67	74257.5
68	56038.0
69	40308.5
70	30588.5
71	24747.5
72	18979.0
73	13023.5
74	8644.5
75	5226.0
76	2855.0
77	1373.0
78	721.0
79	444.0
80	279.5
81	197.5
82	151.5
83	122.5
84	105.0
85	86.0
86	62.5
87	52.5
88	46.5
89	41.0
90	37.0
91	36.0
92	37.5
93	29.5
94	24.5
95	28.0
96	31.5
97	35.0
98	38.0
99	68.0
100	374.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04280812218099699
2	0.0
3	2.183457295344857E-4
4	0.0
5	0.007815129225036214
6	0.0
7	0.0
8	0.0
9	0.0
10-11	2.0392667192371775E-4
12-13	4.1403293996633605E-4
14-15	0.0
16-17	0.0010896687822994613
18-19	2.2658519102635303E-5
20-21	0.0013801097998877869
22-23	3.6253630564216484E-4
24-25	0.0029311884257318217
26-27	0.0
28-29	0.002152559314750354
30-31	0.0
32-33	0.004737690357823746
34-35	0.0
36-37	0.0
38-39	0.0022534927180257298
40-41	0.0
42-43	8.672033220190421E-4
44-45	9.10460494851346E-4
46-47	0.001658191625238311
48-49	0.0013244934348176818
50-51	0.0
52-53	0.003124815770790705
54-55	4.7788876652830826E-4
56-57	0.0033905384039034287
58-59	0.0
60-61	3.089798059450269E-5
62-63	0.003201030789590478
64-65	0.0
66-67	0.0023564859866740716
68-69	4.181526707122697E-4
70-71	0.0027766985227593083
72-73	0.0
74-75	2.2658519102635306E-4
76-77	0.0034667534227032014
78-79	0.0
80-81	0.004006438150420516
82-83	0.0013492118192932842
84-85	0.002700483503959535
86-87	0.0
88-89	0.0010052143020078208
90-91	3.9137442086370077E-5
92-93	3.9137442086370077E-4
94-95	0.0
96-97	0.002381204371149674
98-99	2.6778249848568995E-5
100-101	9.063407641054122E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.4273431E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.566278687823225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.21742332773529	19.955388026893893
2	14.968505570458642	9.450000367546638
3	6.727530441572981	6.370893023985215
4	3.7761553735964273	4.767966915658642
5	2.399723642019211	3.7875172628868268
6	1.642785194597956	3.111396916614539
7	1.159433421899635	2.5619298960962276
8	0.901169952360965	2.275726548905488
9	0.6882436989555938	1.9552763165733524
>10	4.01315754221273	23.226642759514814
>50	0.30544084098576757	6.649642523785807
>100	0.18371808615261875	10.950936168393287
>500	0.012554598460264103	2.6681610250554657
>1k	0.00407682671078851	1.9967849737735812
>5k	5.4321520689812275E-5	0.10642460950909477
>10k+	2.7160760344906137E-5	0.16531266480702575
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT	26714	0.11005448714687263	Illumina Single End PCR Primer 1 (100% over 50bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.119730745933692E-5	0.0	4.119730745933692E-6	0.0	0.0
2	4.9436768951204305E-5	1.6478922983734767E-5	2.4718384475602152E-5	0.0	0.0
3	5.767623044307168E-5	1.6478922983734767E-5	2.883811522153584E-5	0.0	0.0
4	1.1123273014020968E-4	2.4718384475602152E-5	3.707757671340323E-5	0.0	0.0
5	1.2771165312394444E-4	2.883811522153584E-5	3.707757671340323E-5	0.0	0.0
6	1.6066949909141398E-4	2.883811522153584E-5	5.3556499697138E-5	0.0	0.0
7	1.8126815282108245E-4	2.883811522153584E-5	5.767623044307168E-5	8.239461491867384E-6	0.0
8	2.2658519102635306E-4	2.883811522153584E-5	5.767623044307168E-5	8.239461491867384E-6	0.0
9	2.554233062478889E-4	3.2957845967469534E-5	6.591569193493907E-5	8.239461491867384E-6	0.0
10-11	3.851948247448002E-4	3.2957845967469534E-5	7.003542268087275E-5	8.239461491867384E-6	0.0
12-13	4.6758943966347406E-4	3.2957845967469534E-5	8.857421103757437E-5	1.029932686483423E-5	1.6478922983734767E-5
14-15	5.417445930902805E-4	3.2957845967469534E-5	1.0917286476724283E-4	1.2359192237801076E-5	1.6478922983734767E-5
16-17	6.241392080089544E-4	3.2957845967469534E-5	1.6684909521031451E-4	1.2359192237801076E-5	1.6478922983734767E-5
18-19	7.250726112843298E-4	4.119730745933692E-5	2.0392667192371773E-4	1.2359192237801076E-5	1.6478922983734767E-5
20-21	8.218862838137715E-4	4.9436768951204305E-5	2.513035755019552E-4	1.2359192237801076E-5	1.6478922983734767E-5
22-23	9.454782061917822E-4	5.149663432417115E-5	3.233988635557948E-4	1.2359192237801076E-5	1.6478922983734767E-5
24-25	0.0012647573390016434	6.591569193493907E-5	3.460573826584301E-4	1.2359192237801076E-5	1.6478922983734767E-5
26-27	0.0015819766064385375	7.621501879977329E-5	3.563567095232643E-4	1.2359192237801076E-5	1.8538788356701613E-5
28-29	0.0019486326428266362	8.239461491867384E-5	3.7901522862589964E-4	1.6478922983734767E-5	2.059865372966846E-5
30-31	0.0024285812747279114	1.1741232625911023E-4	4.01673747728535E-4	1.6478922983734767E-5	2.059865372966846E-5
32-33	0.002953846944834457	1.483103068536129E-4	4.202125360852366E-4	1.6478922983734767E-5	2.059865372966846E-5
34-35	0.0036006446719460466	1.483103068536129E-4	4.758289011553414E-4	1.6478922983734767E-5	2.059865372966846E-5
36-37	0.004360734994570813	1.565497683454803E-4	5.376248623443468E-4	2.059865372966846E-5	2.059865372966846E-5
38-39	0.005328871719865231	1.8126815282108245E-4	5.747024390577501E-4	2.059865372966846E-5	2.059865372966846E-5
40-41	0.006774897211687956	1.9774707580481722E-4	6.1383988114412E-4	2.059865372966846E-5	2.059865372966846E-5
42-43	0.00861435698974735	2.059865372966846E-4	6.859351691979597E-4	2.059865372966846E-5	2.4718384475602152E-5
44-45	0.010785455092854406	2.1628586416151883E-4	7.08593688300595E-4	2.059865372966846E-5	2.4718384475602152E-5
46-47	0.013005989964912666	2.368845178911873E-4	7.477311303869651E-4	2.059865372966846E-5	2.6778249848569E-5
48-49	0.015875382429455482	2.6160290236678947E-4	7.662699187436667E-4	2.059865372966846E-5	3.089798059450269E-5
50-51	0.019970394790913573	3.151594020639274E-4	7.765692456085009E-4	2.059865372966846E-5	3.2957845967469534E-5
52-53	0.024961448589612238	3.522369787773307E-4	8.054073608300368E-4	2.059865372966846E-5	3.2957845967469534E-5
54-55	0.03053338442348756	3.563567095232643E-4	8.363053414245395E-4	2.059865372966846E-5	3.501771134043638E-5
56-57	0.036677962831047656	3.5841657489623117E-4	8.733829181379426E-4	2.059865372966846E-5	3.707757671340323E-5
58-59	0.043716522810475375	3.5841657489623117E-4	8.960414372405779E-4	2.059865372966846E-5	3.707757671340323E-5
60-61	0.053045653084642215	3.5841657489623117E-4	9.495979369377159E-4	2.059865372966846E-5	3.707757671340323E-5
62-63	0.0652668343424545	3.6665603638809857E-4	0.0010319925518563898	2.2658519102635306E-5	3.707757671340323E-5
64-65	0.08041714416062568	3.893145554907339E-4	0.0010896687822994616	2.4718384475602152E-5	3.707757671340323E-5
66-67	0.09813816596425945	4.366914590689713E-4	0.0010979082437913288	2.6778249848569E-5	3.707757671340323E-5
68-69	0.11953192772789312	4.758289011553414E-4	0.0011123273014020968	2.883811522153584E-5	3.707757671340323E-5
70-71	0.14493830723806617	5.005472856309437E-4	0.0011267463590128647	2.883811522153584E-5	3.9137442086370077E-5
72-73	0.17663345573190703	5.087867471228109E-4	0.0011679436664722017	2.883811522153584E-5	4.119730745933692E-5
74-75	0.21618081102749753	5.129064778687446E-4	0.0011823627240829695	2.883811522153584E-5	4.119730745933692E-5
76-77	0.2656258194401937	5.211459393606121E-4	0.0012050212431856048	2.883811522153584E-5	4.119730745933692E-5
78-79	0.32448647247272133	5.458643238362142E-4	0.0012153205700504391	2.883811522153584E-5	4.119730745933692E-5
80-81	0.3952510874956243	5.582235160740152E-4	0.0012194403007963729	2.883811522153584E-5	4.119730745933692E-5
82-83	0.4806077888206245	5.685228429388495E-4	0.0012400389545260413	2.883811522153584E-5	4.119730745933692E-5
84-85	0.5880730251936778	5.726425736847832E-4	0.001254458012136809	2.883811522153584E-5	4.737690357823746E-5
86-87	0.7191669772600338	5.808820351766506E-4	0.0012668172043746103	2.883811522153584E-5	4.9436768951204305E-5
88-89	0.8746868129190306	5.953010927874185E-4	0.0012853559927313118	3.089798059450269E-5	4.9436768951204305E-5
90	1.0094452654838948	6.303188041278548E-4	0.0012894757234772456	3.2957845967469534E-5	4.9436768951204305E-5
>>END_MODULE
