##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654135_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	29021727
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.602808440724427	32.0	32.0	32.0	32.0	32.0
2	31.682588496542607	32.0	32.0	32.0	32.0	32.0
3	35.71976691807486	37.0	37.0	37.0	32.0	37.0
4	36.344325201598096	37.0	37.0	37.0	37.0	37.0
5	36.42895817330237	37.0	37.0	37.0	37.0	37.0
6	40.08211616765605	41.0	41.0	41.0	37.0	41.0
7	40.09697358809832	41.0	41.0	41.0	37.0	41.0
8	40.23603667693518	41.0	41.0	41.0	37.0	41.0
9	40.21096187694137	41.0	41.0	41.0	41.0	41.0
10-11	40.27490395385499	41.0	41.0	41.0	41.0	41.0
12-13	40.232956519093435	41.0	41.0	41.0	41.0	41.0
14-15	40.2659060399817	41.0	41.0	41.0	41.0	41.0
16-17	40.267988497031894	41.0	41.0	41.0	41.0	41.0
18-19	40.2642838070939	41.0	41.0	41.0	41.0	41.0
20-21	40.195343147566646	41.0	41.0	41.0	41.0	41.0
22-23	40.15420893112253	41.0	41.0	41.0	39.0	41.0
24-25	40.191139762289126	41.0	41.0	41.0	41.0	41.0
26-27	40.16370733209639	41.0	41.0	41.0	41.0	41.0
28-29	40.08243022546522	41.0	41.0	41.0	39.0	41.0
30-31	40.09905142791813	41.0	41.0	41.0	37.0	41.0
32-33	40.10414285476533	41.0	41.0	41.0	37.0	41.0
34-35	40.100779529764026	41.0	41.0	41.0	37.0	41.0
36-37	40.08330014268276	41.0	41.0	41.0	37.0	41.0
38-39	40.058754945906564	41.0	41.0	41.0	37.0	41.0
40-41	40.04660306741911	41.0	41.0	41.0	37.0	41.0
42-43	39.996781290789485	41.0	41.0	41.0	37.0	41.0
44-45	40.0142032209179	41.0	41.0	41.0	37.0	41.0
46-47	40.00179556854077	41.0	41.0	41.0	37.0	41.0
48-49	39.97701203308817	41.0	41.0	41.0	37.0	41.0
50-51	39.9101461294843	41.0	41.0	41.0	37.0	41.0
52-53	39.93088584976353	41.0	41.0	41.0	37.0	41.0
54-55	39.878773306633335	41.0	41.0	41.0	37.0	41.0
56-57	39.87833978660195	41.0	41.0	41.0	37.0	41.0
58-59	39.87086023516106	41.0	41.0	41.0	37.0	41.0
60-61	39.80017383183296	41.0	41.0	41.0	37.0	41.0
62-63	39.76059531191924	41.0	41.0	41.0	37.0	41.0
64-65	39.753453886462374	41.0	41.0	41.0	37.0	41.0
66-67	39.72241042030338	41.0	41.0	41.0	37.0	41.0
68-69	39.642240122374524	41.0	41.0	41.0	37.0	41.0
70-71	39.66591919219694	41.0	41.0	41.0	37.0	41.0
72-73	39.583130614522005	41.0	41.0	41.0	37.0	41.0
74-75	39.62120998174919	41.0	41.0	41.0	37.0	41.0
76-77	39.088741376417744	41.0	39.0	41.0	34.5	41.0
78-79	39.40068492478066	41.0	41.0	41.0	37.0	41.0
80-81	39.60882830990727	41.0	41.0	41.0	37.0	41.0
82-83	39.75009319741723	41.0	41.0	41.0	37.0	41.0
84-85	39.708666906693736	41.0	41.0	41.0	37.0	41.0
86-87	39.73039126858302	41.0	41.0	41.0	37.0	41.0
88-89	39.6692846018433	41.0	41.0	41.0	37.0	41.0
90-91	39.688464766414484	41.0	41.0	41.0	37.0	41.0
92-93	39.64463550015476	41.0	41.0	41.0	37.0	41.0
94-95	39.50506239687252	41.0	41.0	41.0	37.0	41.0
96-97	39.47291170852789	41.0	41.0	41.0	37.0	41.0
98-99	39.52904565258987	41.0	41.0	41.0	37.0	41.0
100-101	38.99821264944019	41.0	39.0	41.0	34.5	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	1.0
15	3.0
16	10.0
17	20.0
18	65.0
19	167.0
20	545.0
21	1446.0
22	3676.0
23	7803.0
24	14251.0
25	24795.0
26	41129.0
27	63730.0
28	93269.0
29	128654.0
30	169505.0
31	215760.0
32	264598.0
33	320665.0
34	385543.0
35	472707.0
36	596883.0
37	808043.0
38	1273144.0
39	2863551.0
40	2.1271762E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	40.75302507044592	12.48502299171766	5.664427671213905	41.09752426662251
2	24.44635358881296	14.164339703147233	31.647337872070814	29.741968835968997
3	20.33219801151048	17.18877033058715	22.498344085450185	39.98068757245218
4	23.508883143766347	24.846234723760823	21.669982503629683	29.97489962884315
5	24.36413243085086	29.388468163869092	23.314256935846718	22.93314246943333
6	20.242534153808283	32.2052957082809	24.923375511043847	22.62879462686697
7	15.868600790021903	25.165680181610146	40.307876922693126	18.657842105674828
8	18.482015215703736	24.55752202479198	31.23066728592685	25.729795473577433
9	18.447703623659333	22.86665077009911	33.89961524333809	24.786030362903478
10-11	21.48472039601117	31.302350031468055	24.60450423717432	22.60842533534645
12-13	21.610857700035123	25.45907787044647	27.498400956058145	25.431663473460265
14-15	21.115473245268966	26.35311468542172	27.373205598688184	25.15820647062113
16-17	21.711760235867985	26.69188039751993	26.805632316205973	24.790727050406115
18-19	21.513793216502755	26.93314107267588	26.55379749054428	24.999268220277084
20-21	21.63232040280593	26.923925693530844	26.68352903887838	24.76022486478485
22-23	21.654197215761833	27.07198127802663	26.596677034416317	24.677144471795216
24-25	21.63118551889048	26.761073294519537	26.411350820220843	25.196390366369144
26-27	21.408665652460996	26.961038535025843	26.640713007878546	24.989582804634612
28-29	21.43176707109331	27.184211572721097	26.521361871005183	24.862659485180412
30-31	21.590377788337683	26.847206577334287	26.553738514596326	25.008677119731708
32-33	21.681371698388606	26.75054853132538	26.616482430802492	24.95159733948352
34-35	21.819340643914455	26.89814564122998	26.335458438691134	24.94705527616443
36-37	21.60818345757088	26.798370890419438	26.297818207635927	25.295627444373757
38-39	21.57444156167438	26.85723720422444	26.520160029603897	25.048161204497283
40-41	21.77166260114712	27.16050580201836	26.40398596760887	24.66384562922565
42-43	21.78391037859325	26.80851143007444	26.431023556937188	24.97655463439512
44-45	21.794870756720847	26.75163046874412	26.5174002896624	24.93609848487263
46-47	21.792048308172998	26.791215654590278	26.472316051524665	24.944419985712056
48-49	21.636028797680456	26.672078962224425	26.41798867315034	25.273903566944778
50-51	21.625181211277383	26.62330373796796	26.654955896390348	25.09655915436431
52-53	22.000449869850957	26.80130648324271	26.27764536548773	24.920598281418606
54-55	21.844220571440168	26.769804355317916	26.30044311859049	25.085531954651426
56-57	21.73721012833322	26.566684593521884	26.65033597692425	25.04576930122065
58-59	21.81065595686123	26.67795875296541	26.58817705332954	24.92320823684382
60-61	21.84480396001517	26.51442590885505	26.5319352166294	25.108834914500378
62-63	21.784969170503587	26.487151991780202	26.609419382024207	25.118459455691998
64-65	21.990222730618804	26.652389347835797	26.46043066234404	24.896957259201354
66-67	21.83476907462835	26.495101207123955	26.43485889194731	25.235270826300383
68-69	21.85614997261389	26.486443672440224	26.686893225420903	24.970513129524978
70-71	22.05950728084626	26.740012044099714	26.51741105716998	24.683069617884044
72-73	22.077452565539804	26.503412887050615	26.357814152786187	25.061320394623394
74-75	21.99382750910907	26.463852625448432	26.55300768775481	24.989312177687687
76-77	21.944183404385274	26.69848007322238	26.467963122938894	24.88937339945345
78-79	21.881653617933154	26.508575501437132	26.424191030495326	25.185579850134392
80-81	21.91314131774491	26.49304836322321	26.55588620709155	25.03792411194034
82-83	22.094122674406773	26.74223280898576	26.376873914836015	24.78677060177145
84-85	22.16428834470773	26.603341035906336	26.229518872739565	25.002851746646364
86-87	22.085471584058798	26.505247614771076	26.49505765331666	24.914223147853463
88-89	22.2799463297598	26.502117211035753	26.44815236338915	24.7697840958153
90-91	22.153375583848796	26.408575303928377	26.390076697801877	25.047972414420943
92-93	22.138135269191643	26.365186168324495	26.380915861286574	25.115762701197287
94-95	22.295896865615717	26.496206767224727	26.27637808878523	24.931518278374327
96-97	22.26523179669379	26.505141284315787	26.092055374913276	25.137571544077147
98-99	22.245133877610343	26.56899155074705	26.34697947071802	24.838895100924592
100-101	22.38400580042292	26.661157749071844	26.22044319274215	24.73439325776308
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	58.0
1	41.0
2	26.5
3	33.5
4	37.5
5	54.5
6	67.0
7	74.5
8	105.5
9	130.0
10	139.0
11	156.5
12	183.5
13	236.5
14	276.0
15	304.0
16	386.5
17	491.5
18	615.5
19	780.5
20	1040.5
21	1417.5
22	2072.5
23	3109.5
24	4685.5
25	7038.5
26	10352.5
27	15430.0
28	22275.5
29	32006.5
30	45877.0
31	63866.5
32	90646.0
33	125182.5
34	165674.5
35	223595.0
36	303516.5
37	407669.5
38	542871.5
39	722337.5
40	926550.5
41	1128883.0
42	1334360.0
43	1533011.0
44	1700470.5
45	1804627.0
46	1859537.0
47	1873571.5
48	1812212.0
49	1700593.5
50	1560723.5
51	1386502.5
52	1238378.0
53	1110262.5
54	963027.0
55	818133.5
56	681889.0
57	551475.0
58	435160.0
59	342240.0
60	272846.5
61	229945.5
62	196734.5
63	163786.0
64	134606.0
65	112415.5
66	96468.5
67	77321.0
68	53745.0
69	35738.5
70	25644.5
71	19124.5
72	15087.0
73	12489.5
74	9225.5
75	5518.5
76	3146.5
77	1207.5
78	143.5
79	44.0
80	19.5
81	9.5
82	5.0
83	3.0
84	0.5
85	0.5
86	1.5
87	1.5
88	1.0
89	1.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.033623085214742734
2	0.0
3	0.0
4	0.018686000319691518
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0027117614330808087
10-11	0.0035507880010035237
12-13	0.0016522104284145463
14-15	0.0
16-17	8.217980963021257E-4
18-19	5.340826202382787E-5
20-21	3.738578341667951E-4
22-23	0.0
24-25	1.7228471620589637E-6
26-27	0.0
28-29	2.2397013106766525E-4
30-31	0.0
32-33	0.0020501881228501668
34-35	6.391762971238755E-4
36-37	3.6696644551855926E-4
38-39	0.004208915616910048
40-41	5.513110918588684E-5
42-43	0.0
44-45	0.0024171545683687257
46-47	1.8606749350236809E-4
48-49	5.289140787521019E-4
50-51	3.273409607912031E-5
52-53	0.0
54-55	9.079404544050739E-4
56-57	3.1355818349473137E-4
58-59	7.666669871162387E-4
60-61	6.891388648235854E-5
62-63	3.049439476844366E-4
64-65	0.0022000758259492965
66-67	0.00206052520582252
68-69	0.00846090241287157
70-71	0.008746895041773358
72-73	6.322849084756397E-4
74-75	0.0013093638431648123
76-77	0.0
78-79	0.0013507121750542274
80-81	0.008924348299465432
82-83	0.0056216502897983986
84-85	1.3610492580265812E-4
86-87	0.01023888068411642
88-89	1.9640457647472184E-4
90-91	0.002141499022439292
92-93	6.253935198274038E-4
94-95	0.01423071755860704
96-97	9.716857994012555E-4
98-99	3.7213498700473617E-4
100-101	0.015617609524064505
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.9021727E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.858649954393332
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.60631037432219	20.264111771060282
2	15.26798353703798	9.728346860318664
3	6.50394100948552	6.216203398356683
4	3.563635914419939	4.541305166496369
5	2.352149668535827	3.7468156465112603
6	1.529947680125458	2.924524055379186
7	1.1218728934400628	2.501894906380056
8	0.8850416874537017	2.2556986652506454
9	0.6672842038951421	1.9132896484792223
>10	4.021881923283687	23.766682396271847
>50	0.3022048967830194	6.5989606832242185
>100	0.15968384604629812	9.577186568188289
>500	0.011914424698637618	2.598542637063519
>1k	0.006067939804044576	3.2018853845259403
>5k	6.857200165656052E-5	0.1276067927439592
>10k+	1.1428666942760086E-5	0.036945419749848885
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.269666377883025E-5	0.0	6.891388648235855E-6	0.0	0.0
2	1.1026221837177368E-4	0.0	6.891388648235855E-6	0.0	0.0
3	1.6194763323354257E-4	0.0	6.891388648235855E-6	0.0	2.411986026882549E-5
4	2.515356856606087E-4	3.4456943241179274E-6	1.0337082972353781E-5	0.0	2.411986026882549E-5
5	2.9977540619825966E-4	3.4456943241179274E-6	1.0337082972353781E-5	3.4456943241179274E-6	2.411986026882549E-5
6	3.445694324117927E-4	3.4456943241179274E-6	1.7228471620589636E-5	3.4456943241179274E-6	2.411986026882549E-5
7	3.721349870047361E-4	6.891388648235855E-6	1.7228471620589636E-5	3.4456943241179274E-6	2.411986026882549E-5
8	4.410488734870947E-4	6.891388648235855E-6	1.7228471620589636E-5	3.4456943241179274E-6	2.756555459294342E-5
9	4.686144280800381E-4	1.0337082972353781E-5	1.7228471620589636E-5	3.4456943241179274E-6	3.1011248917061345E-5
10-11	5.271912315900429E-4	1.5505624458530673E-5	1.89513187826486E-5	3.4456943241179274E-6	3.445694324117927E-5
12-13	5.823223407759297E-4	2.411986026882549E-5	2.0674165944707563E-5	6.891388648235855E-6	4.307117905147409E-5
14-15	6.736332403650548E-4	2.756555459294342E-5	2.0674165944707563E-5	6.891388648235855E-6	5.168541486176891E-5
16-17	7.511613626577081E-4	3.1011248917061345E-5	2.0674165944707563E-5	6.891388648235855E-6	5.168541486176891E-5
18-19	8.390265679227152E-4	3.1011248917061345E-5	2.0674165944707563E-5	6.891388648235855E-6	5.513110918588684E-5
20-21	9.337831618359583E-4	3.1011248917061345E-5	2.0674165944707563E-5	6.891388648235855E-6	6.374534499618165E-5
22-23	0.0010182026727768474	3.1011248917061345E-5	2.0674165944707563E-5	6.891388648235855E-6	6.891388648235854E-5
24-25	0.0011301877383106801	3.445694324117927E-5	2.0674165944707563E-5	6.891388648235855E-6	6.891388648235854E-5
26-27	0.0012611241226271614	3.6179790403238235E-5	2.0674165944707563E-5	6.891388648235855E-6	6.891388648235854E-5
28-29	0.0014885399480189447	3.79026375652972E-5	2.0674165944707563E-5	6.891388648235855E-6	7.063673364441751E-5
30-31	0.001843446463403091	3.79026375652972E-5	2.0674165944707563E-5	6.891388648235855E-6	7.235958080647647E-5
32-33	0.002367192000669016	4.307117905147409E-5	2.0674165944707563E-5	6.891388648235855E-6	7.235958080647647E-5
34-35	0.0031028477388681938	4.996256769970995E-5	2.0674165944707563E-5	6.891388648235855E-6	7.58052751305944E-5
36-37	0.004102099092862392	5.513110918588684E-5	2.0674165944707563E-5	6.891388648235855E-6	7.58052751305944E-5
38-39	0.005513110918588683	5.513110918588684E-5	2.0674165944707563E-5	6.891388648235855E-6	7.752812229265336E-5
40-41	0.0074737109890117845	5.513110918588684E-5	2.0674165944707563E-5	6.891388648235855E-6	7.925096945471232E-5
42-43	0.010063150273586407	6.202249783412269E-5	2.0674165944707563E-5	6.891388648235855E-6	7.925096945471232E-5
44-45	0.012857608370446045	7.752812229265336E-5	2.411986026882549E-5	6.891388648235855E-6	8.269666377883025E-5
46-47	0.016063826939037778	1.0337082972353782E-4	2.411986026882549E-5	6.891388648235855E-6	8.614235810294818E-5
48-49	0.02036233060837489	1.0337082972353782E-4	2.411986026882549E-5	6.891388648235855E-6	8.786520526500714E-5
50-51	0.026061509020465945	1.0681652404765575E-4	2.411986026882549E-5	6.891388648235855E-6	8.95880524270661E-5
52-53	0.03289948940667797	1.1198506553383264E-4	2.411986026882549E-5	6.891388648235855E-6	8.95880524270661E-5
54-55	0.040457619906630646	1.137079126958916E-4	2.5842707430884456E-5	6.891388648235855E-6	9.475659391324299E-5
56-57	0.049049458703818695	1.3093638431648123E-4	3.445694324117927E-5	6.891388648235855E-6	9.99251353994199E-5
58-59	0.05934002480279688	1.3610492580265812E-4	4.479402621353305E-5	6.891388648235855E-6	1.0164798256147886E-4
60-61	0.07193920609893409	1.4471916161295294E-4	4.479402621353305E-5	6.891388648235855E-6	1.0337082972353782E-4
62-63	0.0878462539462245	1.4471916161295294E-4	4.479402621353305E-5	6.891388648235855E-6	1.0337082972353782E-4
64-65	0.10632551260646894	1.4471916161295294E-4	4.479402621353305E-5	6.891388648235855E-6	1.0337082972353782E-4
66-67	0.12816087753840424	1.5505624458530672E-4	4.8239720537650985E-5	6.891388648235855E-6	1.0337082972353782E-4
68-69	0.15367279831417335	1.5505624458530672E-4	5.3408262023827875E-5	1.0337082972353781E-5	1.0337082972353782E-4
70-71	0.18435153772895735	1.5505624458530672E-4	5.513110918588684E-5	1.0337082972353781E-5	1.0337082972353782E-4
72-73	0.2224023401501916	1.5505624458530672E-4	5.513110918588684E-5	1.2059930134412746E-5	1.0509367688559679E-4
74-75	0.27036295944758904	1.6711617471971946E-4	6.202249783412269E-5	1.378277729647171E-5	1.0681652404765575E-4
76-77	0.3274529458567369	2.0674165944707565E-4	6.202249783412269E-5	1.378277729647171E-5	1.0681652404765575E-4
78-79	0.39482660697621474	2.0674165944707565E-4	6.202249783412269E-5	1.378277729647171E-5	1.0681652404765575E-4
80-81	0.4748011722389919	2.0674165944707565E-4	6.546819215824062E-5	1.378277729647171E-5	1.1543075985795057E-4
82-83	0.5720197147468171	2.0674165944707565E-4	6.546819215824062E-5	1.378277729647171E-5	1.2404499566824538E-4
84-85	0.6920969934008407	2.1191020093325254E-4	6.546819215824062E-5	1.378277729647171E-5	1.2404499566824538E-4
86-87	0.8393022234686447	2.2397013106766528E-4	6.546819215824062E-5	1.378277729647171E-5	1.2404499566824538E-4
88-89	1.0130892623998564	2.3086151971590113E-4	6.546819215824062E-5	1.378277729647171E-5	1.274906899923633E-4
90	1.159662207559185	2.653184629570804E-4	6.546819215824062E-5	1.378277729647171E-5	1.274906899923633E-4
>>END_MODULE
