##FastQC	0.11.9
>>Basic Statistics	pass
#Measure	Value
Filename	SRR19654131_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	28494240
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.42832196963316	32.0	32.0	32.0	32.0	32.0
2	31.454340245607533	32.0	32.0	32.0	32.0	32.0
3	34.74463540701559	37.0	32.0	37.0	32.0	37.0
4	35.74787693933932	37.0	37.0	37.0	32.0	37.0
5	35.90429342912813	37.0	37.0	37.0	37.0	37.0
6	39.25549812874462	41.0	41.0	41.0	37.0	41.0
7	39.52269125268826	41.0	41.0	41.0	37.0	41.0
8	39.61631245472769	41.0	41.0	41.0	37.0	41.0
9	39.4539321280371	41.0	41.0	41.0	37.0	41.0
10-11	39.48593296399553	41.0	41.0	41.0	37.0	41.0
12-13	39.60042905162587	41.0	41.0	41.0	37.0	41.0
14-15	39.63007227074665	41.0	41.0	41.0	37.0	41.0
16-17	39.41834597097518	41.0	41.0	41.0	37.0	41.0
18-19	39.49888273910798	41.0	41.0	41.0	37.0	41.0
20-21	39.52567889159353	41.0	41.0	41.0	37.0	41.0
22-23	39.34957928339201	41.0	41.0	41.0	37.0	41.0
24-25	39.34918465977685	41.0	41.0	41.0	37.0	41.0
26-27	39.314808694669516	41.0	41.0	41.0	37.0	41.0
28-29	39.12911858677403	41.0	41.0	41.0	37.0	41.0
30-31	39.30906591998944	41.0	41.0	41.0	37.0	41.0
32-33	39.4281836434311	41.0	41.0	41.0	37.0	41.0
34-35	39.31781637271252	41.0	41.0	41.0	37.0	41.0
36-37	39.084433818905154	41.0	41.0	41.0	37.0	41.0
38-39	39.09666153580513	41.0	41.0	41.0	37.0	41.0
40-41	39.059283525372145	41.0	41.0	41.0	37.0	41.0
42-43	38.94546076680761	41.0	41.0	41.0	37.0	41.0
44-45	38.97542245380119	41.0	41.0	41.0	37.0	41.0
46-47	38.949356255860835	41.0	41.0	41.0	37.0	41.0
48-49	38.98552618002796	41.0	41.0	41.0	37.0	41.0
50-51	38.894974791396436	41.0	41.0	41.0	32.0	41.0
52-53	38.97780723402344	41.0	41.0	41.0	37.0	41.0
54-55	38.85392454755768	41.0	41.0	41.0	32.0	41.0
56-57	38.839569698998815	41.0	41.0	41.0	32.0	41.0
58-59	38.83198239363465	41.0	41.0	41.0	32.0	41.0
60-61	38.856869212865476	41.0	41.0	41.0	32.0	41.0
62-63	38.86892331572977	41.0	41.0	41.0	32.0	41.0
64-65	38.8672291136735	41.0	41.0	41.0	32.0	41.0
66-67	38.81778457681271	41.0	41.0	41.0	32.0	41.0
68-69	38.775416399946096	41.0	41.0	41.0	32.0	41.0
70-71	38.73286202755364	41.0	41.0	41.0	32.0	41.0
72-73	38.68751814401788	41.0	41.0	41.0	32.0	41.0
74-75	38.66542932887489	41.0	41.0	41.0	32.0	41.0
76-77	37.839914716097006	41.0	39.0	41.0	29.5	41.0
78-79	38.39876422392736	41.0	39.0	41.0	32.0	41.0
80-81	38.81767820443711	41.0	41.0	41.0	32.0	41.0
82-83	38.858754909764215	41.0	41.0	41.0	32.0	41.0
84-85	38.73892669185071	41.0	41.0	41.0	32.0	41.0
86-87	38.77758096022214	41.0	41.0	41.0	32.0	41.0
88-89	38.65916816872463	41.0	41.0	41.0	32.0	41.0
90-91	38.56572340585325	41.0	41.0	41.0	32.0	41.0
92-93	38.550594014790356	41.0	41.0	41.0	32.0	41.0
94-95	38.37710658364638	41.0	39.0	41.0	32.0	41.0
96-97	38.34500804373094	41.0	37.0	41.0	32.0	41.0
98-99	38.27105307949957	41.0	37.0	41.0	32.0	41.0
100-101	37.182515799684424	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	26.0
13	101.0
14	328.0
15	1352.0
16	6447.0
17	17720.0
18	32033.0
19	43111.0
20	52741.0
21	62955.0
22	74600.0
23	87762.0
24	102216.0
25	118020.0
26	136005.0
27	157055.0
28	180622.0
29	209887.0
30	244703.0
31	288422.0
32	340276.0
33	408205.0
34	505323.0
35	644962.0
36	839638.0
37	1171357.0
38	1950134.0
39	4920749.0
40	1.5897489E7
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	28.10634613983417	20.599649193888606	12.664935238872596	38.62906942740463
2	27.3538395243015	25.230687892228204	31.434923876987842	15.980548706482454
3	21.0180330647717	28.284000327089043	28.29810516474303	22.399861443396222
4	24.73508651302047	30.953581864777576	23.076366958506096	21.234964663695862
5	28.507157212986666	32.2930248851006	21.740030059522347	17.459787842390384
6	22.81259955012687	37.41086792496799	21.605976118814013	18.170556406091134
7	22.538573329406255	22.1984762155586	35.29678643797873	19.96616401705641
8	22.847468915622496	24.34671274440086	28.15108458598779	24.654733753988857
9	24.028946772435294	22.96130843280392	29.84357390025846	23.166170894502326
10-11	25.209138165637697	30.2563160070852	23.348235053351253	21.186310773925847
12-13	25.859032912641215	25.185607902171515	25.751186851544123	23.204172333643147
14-15	25.01435470345444	27.08385201813907	25.672674855239887	22.229118423166604
16-17	26.12060531105451	26.49957704564501	25.054636100713722	22.32518154258675
18-19	25.640804785300965	27.06266246597998	25.108642926890223	22.18788982182883
20-21	25.928627552486518	27.049431976467442	25.07569947288645	21.9462409981596
22-23	25.77473641790176	26.837547957241153	25.230370773163507	22.157344851693576
24-25	25.13023690837073	26.988234036748793	25.684009095195155	22.197519959685327
26-27	25.49908471601663	27.022972749648353	25.50846472942101	21.969477804914007
28-29	25.6661332760546	26.657037733327684	25.359614863724794	22.317214126892914
30-31	25.319980867721313	26.88468256817234	25.537060805003524	22.258275759102816
32-33	25.533526503096738	27.076153504015256	25.28836744899848	22.10195254388953
34-35	25.654052724178484	26.785346899796824	25.28539480661964	22.275205569405053
36-37	25.373195435573383	26.902114093818398	25.531462913331772	22.19322755727645
38-39	25.572780668226546	27.074782133553597	25.275164738732688	22.07727245948717
40-41	25.739029926636288	26.67200025888203	25.264315182974773	22.324654631506913
42-43	25.330920285263304	26.82666131941729	25.617761652103503	22.224656743215903
44-45	25.51663412969577	26.895469325148795	25.49791656045976	22.089979984695677
46-47	25.714238083543496	26.701252351344863	25.42298762652339	22.161521938588255
48-49	25.283850181499538	26.778959520566815	25.688388120139223	22.24880217779442
50-51	25.68699168522367	26.8903973394934	25.275235668278757	22.14737530700418
52-53	25.838533625526104	26.656139556198443	25.29836903492257	22.20695778335288
54-55	25.386207453528943	26.887557219802627	25.54669368597613	22.1795416406923
56-57	25.40421195437572	27.08988768304181	25.470791757371952	22.035108605210514
58-59	25.6882725129074	26.62138432881054	25.484538668788204	22.205804489493854
60-61	25.48321335481514	26.73358655300969	25.56814303351847	22.2150570586567
62-63	25.615905742469153	26.828536299618467	25.49531483967459	22.06024311823779
64-65	25.75287628915929	26.634360314628157	25.480942157715198	22.131821238497356
66-67	25.42481345908981	26.864619858508966	25.686668549151914	22.023898133249315
68-69	25.70089231152202	27.010335198256385	25.42957615816959	21.85919633205201
70-71	25.952541705204684	26.779898391948148	25.187526145105153	22.08003375774201
72-73	25.393673694384013	26.96726993866376	25.591418454744506	22.047637912207716
74-75	25.510622179293797	26.966509831507135	25.47323930220291	22.049628686996158
76-77	25.758618274611106	26.737701223343024	25.432406454407197	22.071274047638674
78-79	25.41883153993783	26.869446754221737	25.778992416148185	21.93272928969225
80-81	25.695230580601507	27.005936130901713	25.451509337845497	21.84732395065128
82-83	25.85829355093964	26.724267715543586	25.470687326433794	21.946751407082985
84-85	25.556968494728604	26.889903060851907	25.703241834557787	21.849886609861706
86-87	25.628742601132803	27.040701773559224	25.571213964606237	21.75934166070174
88-89	25.754199716783976	26.871536270372253	25.425941916497674	21.948322096346097
90-91	25.459670379472087	27.01685655473664	25.71172359822553	21.81174946756574
92-93	25.798366335300077	27.099264579745373	25.389033199340034	21.713335885614516
94-95	25.991110679945873	26.78882350901336	25.39314852536574	21.826917285675034
96-97	25.64927061724796	26.89256588554173	25.704298108970892	21.753865388239415
98-99	25.814502549042786	27.191486677071495	25.41673938552542	21.5772713883603
100-101	26.110690251356022	26.862316700718313	25.434009538462128	21.592983509463537
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	67.0
1	41.5
2	15.5
3	17.5
4	22.5
5	36.5
6	44.5
7	51.0
8	58.0
9	71.0
10	100.5
11	123.0
12	171.0
13	229.0
14	265.5
15	346.0
16	461.5
17	599.5
18	783.5
19	1014.0
20	1302.5
21	1691.0
22	2286.5
23	3078.5
24	4079.0
25	5590.0
26	7534.0
27	10071.5
28	13931.5
29	18863.5
30	25363.0
31	34715.0
32	48326.5
33	67272.0
34	97306.0
35	146826.0
36	213648.5
37	301817.0
38	431462.5
39	609401.5
40	822758.0
41	1055656.5
42	1306528.0
43	1541646.0
44	1737840.0
45	1865935.5
46	1920662.0
47	1934027.0
48	1878947.5
49	1742790.5
50	1562616.0
51	1401626.0
52	1258271.5
53	1099870.5
54	945196.5
55	800741.0
56	655273.5
57	531042.0
58	431980.5
59	354445.0
60	295498.0
61	247503.0
62	204272.0
63	164307.5
64	138698.0
65	119699.0
66	103381.5
67	83137.0
68	59119.0
69	44220.5
70	40465.5
71	33900.5
72	24284.5
73	16909.5
74	9119.0
75	4332.0
76	2376.0
77	1317.5
78	816.5
79	554.0
80	354.5
81	263.0
82	216.0
83	173.0
84	150.5
85	130.5
86	110.5
87	95.0
88	91.0
89	82.0
90	70.0
91	61.0
92	54.0
93	52.0
94	50.5
95	45.0
96	48.0
97	51.5
98	66.5
99	140.5
100	609.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0075383656486363556
2	0.006102987831926733
3	0.0016494561707910088
4	1.1230339886236657E-4
5	0.014388872979240717
6	0.0015055674409986018
7	0.005250183896815637
8	0.009394881211079854
9	0.0034287631465166296
10-11	0.0066381837171302
12-13	0.004606194093964254
14-15	0.009501920388120547
16-17	1.0703917704069313E-4
18-19	0.012844701244883178
20-21	0.006325839889044242
22-23	0.008608757419043288
24-25	0.011835725395729102
26-27	0.00985988747199434
28-29	0.006975093913717299
30-31	0.010958355092116862
32-33	0.005309845077461269
34-35	0.005018558136662007
36-37	0.009124651157567285
38-39	0.010491594090595153
40-41	0.00876668407369349
42-43	0.012562188007120034
44-45	0.00804548568412423
46-47	0.004342983002880582
48-49	0.009986228795714503
50-51	0.010438951872378418
52-53	0.0073383252194127654
54-55	0.010567047936705804
56-57	0.012697303033876318
58-59	0.005874871552987551
60-61	0.0023408239700374533
62-63	0.01298332575285391
64-65	0.005244919674993963
66-67	0.006188970121680733
68-69	0.015490850080577687
70-71	0.006362689441795955
72-73	0.0059748917675993465
74-75	0.013769449544890474
76-77	0.006539918243125629
78-79	0.012769247398772523
80-81	2.281162789391821E-5
82-83	0.007196191230227582
84-85	0.01673847065231429
86-87	0.0021214813941343932
88-89	0.010226628258904255
90-91	0.003688464756385852
92-93	0.011888367613945837
94-95	0.010430178169342295
96-97	0.004244717528876011
98-99	0.009343993733470344
100-101	0.010386309654161683
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2.849424E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.47188115342792
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.035608073524955	21.099203818710887
2	15.364807015637455	10.285779887455448
3	6.919200898002324	6.947960104038768
4	3.8505847876028994	5.15545265527367
5	2.3722213976821798	3.9701356346418297
6	1.6029075018407175	3.2191397640930326
7	1.141448080184075	2.6744490137910875
8	0.8694024136613256	2.328042741166022
9	0.6820430476034223	2.0546337447813183
>10	3.7516901946590604	22.807612920242576
>50	0.2541567446288713	5.877436110290106
>100	0.14073991789523155	8.838994140668914
>500	0.011224576724842674	2.5735993908693047
>1k	0.003921099754846847	2.071703700264775
>5k	4.4250597806837356E-5	0.0958563737122196
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.8604293358938505E-5	3.5094812144489553E-6	2.4566368501142688E-5	2.1056887286693733E-5	0.0
2	4.2113774573387467E-5	3.5094812144489553E-6	3.1585330930040597E-5	2.4566368501142688E-5	0.0
3	5.264221821673433E-5	3.5094812144489553E-6	3.509481214448955E-5	2.4566368501142688E-5	1.0528443643346867E-5
4	9.826547400457075E-5	3.5094812144489553E-6	4.9132737002285375E-5	2.4566368501142688E-5	1.0528443643346867E-5
5	1.0528443643346866E-4	3.5094812144489553E-6	4.9132737002285375E-5	2.4566368501142688E-5	1.0528443643346867E-5
6	1.2634132372016239E-4	3.5094812144489553E-6	5.264221821673433E-5	2.4566368501142688E-5	1.0528443643346867E-5
7	1.333602861490603E-4	3.5094812144489553E-6	7.01896242889791E-5	2.4566368501142688E-5	1.4037924857795821E-5
8	1.649456170791009E-4	3.5094812144489553E-6	7.720858671787701E-5	2.4566368501142688E-5	1.7547406072244775E-5
9	1.965309480091415E-4	3.5094812144489553E-6	9.47559927901218E-5	2.4566368501142688E-5	1.7547406072244775E-5
10-11	2.597016098692227E-4	3.5094812144489553E-6	1.2283184250571345E-4	2.4566368501142688E-5	1.7547406072244775E-5
12-13	3.3515545597987524E-4	3.5094812144489553E-6	1.4213398918518267E-4	2.4566368501142688E-5	1.9302146679469256E-5
14-15	4.246472269483236E-4	3.5094812144489553E-6	1.5968139525742746E-4	2.4566368501142688E-5	2.1056887286693733E-5
16-17	5.264221821673433E-4	3.5094812144489553E-6	2.439089444042024E-4	2.4566368501142688E-5	2.1056887286693733E-5
18-19	6.948772804608932E-4	3.5094812144489553E-6	3.0006064383538566E-4	2.8075849715591642E-5	2.1056887286693733E-5
20-21	8.370112696460758E-4	3.5094812144489553E-6	3.509481214448955E-4	2.8075849715591642E-5	2.1056887286693733E-5
22-23	0.0010195042927974216	3.5094812144489553E-6	4.246472269483236E-4	2.983059032281612E-5	2.1056887286693733E-5
24-25	0.0013581692299917457	3.5094812144489553E-6	4.404398924133439E-4	3.1585330930040597E-5	2.1056887286693733E-5
26-27	0.0018494966000145996	5.264221821673433E-6	4.4570411423501733E-4	3.1585330930040597E-5	2.1056887286693733E-5
28-29	0.002432070481613126	1.0528443643346867E-5	4.930821106300782E-4	3.1585330930040597E-5	2.1056887286693733E-5
30-31	0.0030514939159633665	1.2283184250571344E-5	5.07120035487874E-4	3.1585330930040597E-5	2.1056887286693733E-5
32-33	0.0038393724486071572	1.9302146679469256E-5	5.123842573095474E-4	3.509481214448955E-5	2.1056887286693733E-5
34-35	0.004699195346147151	2.1056887286693733E-5	5.755549191696287E-4	3.8604293358938505E-5	2.1056887286693733E-5
36-37	0.0057853797820191025	2.281162789391821E-5	6.071402500996692E-4	3.8604293358938505E-5	2.1056887286693733E-5
38-39	0.007087397312579666	2.4566368501142688E-5	6.457445434586077E-4	3.8604293358938505E-5	2.281162789391821E-5
40-41	0.008650871193616676	2.8075849715591642E-5	6.843488368175462E-4	3.8604293358938505E-5	2.4566368501142688E-5
42-43	0.010430178169342295	2.8075849715591642E-5	7.475194986776275E-4	4.9132737002285375E-5	2.8075849715591642E-5
44-45	0.012530602676189995	2.8075849715591642E-5	7.580479423209743E-4	4.9132737002285375E-5	2.8075849715591642E-5
46-47	0.01471525473218447	3.8604293358938505E-5	7.966522356799128E-4	4.9132737002285375E-5	2.8075849715591642E-5
48-49	0.017436857413989634	3.8604293358938505E-5	8.352565290388514E-4	4.9132737002285375E-5	2.8075849715591642E-5
50-51	0.02080946886107508	3.8604293358938505E-5	8.405207508605248E-4	4.9132737002285375E-5	2.983059032281612E-5
52-53	0.024936618769267054	3.8604293358938505E-5	8.685966005761164E-4	4.9132737002285375E-5	3.509481214448955E-5
54-55	0.029551586566267427	4.2113774573387467E-5	8.896534878628102E-4	4.9132737002285375E-5	3.8604293358938505E-5
56-57	0.034566635221714985	4.562325578783642E-5	9.194840781856263E-4	4.9132737002285375E-5	3.8604293358938505E-5
58-59	0.04094336258836874	4.73779963950609E-5	9.370314842578711E-4	4.9132737002285375E-5	3.8604293358938505E-5
60-61	0.04891690390759676	5.264221821673433E-5	9.966926649035033E-4	5.264221821673433E-5	4.2113774573387467E-5
62-63	0.058706601755302124	5.264221821673433E-5	0.001138826654088686	5.439695882395881E-5	4.2113774573387467E-5
64-65	0.07079325505786432	5.264221821673433E-5	0.0012019973159487674	5.966118064563224E-5	4.2113774573387467E-5
66-67	0.08516107114981836	5.264221821673433E-5	0.001219544722021012	6.317066186008119E-5	4.2113774573387467E-5
68-69	0.10244351139037224	5.6151699431183284E-5	0.0012406016093077057	6.492540246730568E-5	4.3868515180611944E-5
70-71	0.12350566289888763	5.6151699431183284E-5	0.001252884793558277	6.668014307453015E-5	4.73779963950609E-5
72-73	0.15023562656873812	5.6151699431183284E-5	0.0012774511620594197	6.668014307453015E-5	5.439695882395881E-5
74-75	0.18455133388362	5.6151699431183284E-5	0.0012844701244883177	7.01896242889791E-5	5.966118064563224E-5
76-77	0.22657561668603898	5.6151699431183284E-5	0.0012879796057027667	7.01896242889791E-5	5.966118064563224E-5
78-79	0.2773823762276165	5.966118064563224E-5	0.001300262789953338	7.545384611065253E-5	6.317066186008119E-5
80-81	0.34104787493893507	5.966118064563224E-5	0.001310791233596685	7.720858671787701E-5	6.317066186008119E-5
82-83	0.41831787757806493	5.966118064563224E-5	0.0013195649366328072	7.720858671787701E-5	6.317066186008119E-5
84-85	0.513240570725873	6.141592125285671E-5	0.0013213196772400317	7.720858671787701E-5	6.317066186008119E-5
86-87	0.6335210203886821	6.668014307453015E-5	0.0013283386396689295	7.720858671787701E-5	6.317066186008119E-5
88-89	0.7786608802340402	7.720858671787701E-5	0.001340621823919501	7.720858671787701E-5	6.317066186008119E-5
90	0.9025332839198378	7.720858671787701E-5	0.00134413130513395	7.720858671787701E-5	6.317066186008119E-5
>>END_MODULE
